Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,293,729 | C→G | 13.6% | A111G (GCG→GGG) | lpoA → | OM lipoprotein stimulator of MrcA transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,293,729 | 0 | C | G | 13.6% | 236.3 / 4.2 | 113 | A111G (GCG→GGG) | lpoA | OM lipoprotein stimulator of MrcA transpeptidase |
Reads supporting (aligned to +/- strand): ref base C (36/57); new base G (14/4); total (51/62) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.60e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 |
Only 100 of 116 total aligned reads displayed.
TCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAA > NC_000913/3293500‑3293966 | tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCCACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGGAGAGA‑TTAAACA‑TGCGCA‑G‑aaaaa > 1:62910/1‑237 (MQ=255) tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑CAGAACTTAACGATGCTCAACGTCGC‑GAG‑AAAACAATG‑CCGGC‑GGGAGAGA‑TTAAACT‑GGCGCA‑G‑aaaaa > 1:195385/1‑237 (MQ=255) tCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAAAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAAAACTG‑CTTGC‑GGTAGAGA‑TTTAAAT‑GGGGCA‑G‑AAAGa > 1:829315/1‑239 (MQ=255) cACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACCGAACGATGCTCAGCGGCGC‑GAG‑AAAAAACTG‑CCGGC‑GGGAAAGA‑ATAAAAA‑GGGGCA‑G‑aaaaaaa > 2:265064/1‑235 (MQ=255) tGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGCGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCGCAGCGACGC‑GGG‑AAAAACCTG‑CTGGC‑GGGAGAAG‑TTAAACC‑GGCGGA‑G‑AAAAA‑TATTg > 1:467890/1‑239 (MQ=255) tGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAAACAACGC‑AAGAACTGGACGATGCTCAGCGGCGC‑GAG‑GAAACACCG‑CCGGCGGGTAGGGA‑TAAAACG‑GGGCGA‑G‑AAAAA‑tttt > 2:115751/1‑239 (MQ=255) cTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTACAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGGCGC‑CAG‑CAAACACTG‑CTGGC‑GGTAGAGC‑TTAACAT‑GGAGCA‑G‑AAAAA‑TTTTGCt > 1:401924/1‑239 (MQ=255) atatGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGCTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GGG‑AAAACACTG‑CTGGC‑GGTAGAAG‑TTAAACA‑GGGGCA‑C‑AAAAA‑ATTTATTG‑gc > 1:512942/1‑239 (MQ=255) ttcAGGGCCCGGCGCAGGCTGTTTTTGCCTTTTATCTTCAGCAGTTGCAGCAAAGCTTTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CGGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑gcgcg < 2:455572/237‑1 (MQ=255) gCAGGGCACGGGCCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATTCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑gcgcgc < 1:461883/239‑1 (MQ=255) gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGGTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAACCAACTACCCC‑AAGAACTGAACGGTGCTCCGCGTCCC‑GCG‑AAAAAACTG‑CTGGC‑GGGAGGGG‑TTAAACC‑GGGGCC‑G‑AAAAA‑atttttcggccgccaaa > 2:623796/1‑226 (MQ=255) gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTTAGTTGTTTAACCCACTACCGA‑CAGAACTGAACGATGGCCCGCTTCGG‑GAGCAAAACA‑TG‑CTGGC‑GGT‑GAGAGTTAAACA‑GGGACACG‑AAAAA‑ATTTGCTC‑GCGGGC‑ac > 2:573234/1‑238 (MQ=255) gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GGG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCC‑G‑AAAGA‑TTTTGCTG‑gccggccaa > 1:795727/1‑232 (MQ=255) gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTAACGC‑AAGAAATGAACGACGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAAAGA‑TTAAAAC‑GGCGCA‑A‑AAAGA‑GTTTGCtaggggcccaa > 2:88965/1‑229 (MQ=255) gggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGGTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GGG‑AAAACAATG‑CTGGC‑GGTAGGGG‑TTAAAAC‑GGCGCC‑G‑AAAGA‑ATTTTCTG‑gtgccgaaa > 1:98071/1‑234 (MQ=255) ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGCGTTGTTTAACCAACTACCGC‑CAAAACTGAAAGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGG‑GGGAGGAA‑ATAAAAT‑TGCGCC‑G‑AAAAA‑ttttttctgcgggccaaa > 1:764984/1‑225 (MQ=255) ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTTGTTAACCAACTACCCC‑AACAACTTAACCATGCTCCGCGTCCG‑GGG‑GAAAAACAC‑CCGGC‑GGGAGAGA‑ATAAAAA‑GGGGCA‑G‑AAAGA‑TTTTgtctagccgcaaaa > 2:214790/1‑226 (MQ=255) ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTCCCGC‑AGAAACTGAACGATGCTCAAGGTCCC‑GAG‑AAAACACTG‑CTTGC‑GGAAGTAG‑TGTAACT‑GGGGCG‑T‑GAAGG‑TTTTGCTG‑GGGCGC‑aaaa > 1:449168/1‑239 (MQ=255) ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑AGCGCA‑G‑AAAGA‑ATTTGCTG‑GGGGGC‑caaa > 1:709097/1‑239 (MQ=255) ggCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAAAACTG‑CTTGC‑GGTAGGGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑aaa > 1:208543/1‑238 (MQ=255) cGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGGTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTg < 1:623796/239‑1 (MQ=255) cgcAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGGAGGGA‑TTAAACT‑GGGGCC‑GAAAAGA‑TTTT‑CTG‑GCGCGC‑aaaaactttcg > 1:136246/1‑232 (MQ=255) ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCCC‑GGG‑GAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCC‑G‑AAAGA‑TTTTGCTGTGCG‑GC‑AAAA‑C‑TTTCTGGCGAAA‑ATCACAcc > 1:824564/1‑238 (MQ=255) tttttttttagagagatgagcaaaaGGTCTGATGTTACCAGGATCAACGGGAAATTACCGGCAATTCGGGCCCTGGTAAAAGAAGGTAAAACGGGGAAGGCGGTTGAGTTGTTTAACAAACTACCGA‑AAGAACTGAACGAGGCTCAGCGCGGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGc < 2:395778/217‑1 (MQ=255) tttttacTTCAGCAGATGCAGCAAACCTCTGATGATACCAGGATCAACTGGCAATTCCTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGc < 1:429913/233‑1 (MQ=255) cTTCCGCAGATGCAGGAAAGCTCTGATGAATCCAGGATCAACTGGCAATTACCCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGAttt < 1:214790/239‑1 (MQ=255) tCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAACAACTACCGC‑AAGAACTGAACGATGCCCAGCGTCGC‑GAG‑AAAAAACTGCCGGGG‑GGTAGAG‑‑TTTAACT‑TGGGCA‑C‑AAAAATTTTTTTTG‑GCGCGC‑AAAA‑C‑TTTGTGGGAAAA‑AACAccccccccgttata > 2:683997/1‑229 (MQ=255) aTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTTTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGG‑TTAAAAT‑GGCGCG‑G‑AAAGA‑TTTTTCAG‑GCGCGC‑CAAA‑C‑TTGCTGGCGCAA‑ATCACAACTGCCGATTTTG‑Aaaaaaa > 1:437955/1‑239 (MQ=255) gcagcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAACAACTACCGC‑AAGAACTGAAAGGTGCCCAGCGGCCG‑GGG‑AAAACAACG‑CTTGC‑GGTAGAGA‑TTAAACA‑GGGGCC‑GTAAAGA‑TTTT‑TTT‑GCGCGC‑CAAA‑C‑ATTCTGGCGGAA‑ATCACACCCCCCCCATTAG‑Aaaaaaaaa > 2:718365/1‑237 (MQ=255) aagccttgatgaTACCAGGATCAACTGGCAATTACTCGCAATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAg < 1:227046/233‑1 (MQ=255) aactctgtgtatcccAGGATCAATGTGCAATTGCCCGCCATTTGGGCCGTGGGAAAAGAAGGTAAAACCGGGCTGGGGGTTGAGTTGTTAAACCAACTACCGA‑AAAAACTGAACGATGCTAAGCGTGGG‑GAG‑AAAACACGG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGT‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAgc < 2:627968/226‑1 (MQ=255) tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAAAACTG‑CTGGC‑GGTAGAGA‑TTAAAAC‑TGCGCA‑G‑AAAAA‑TTTTTCTG‑GGGCCC‑AAAA‑A‑CTTCTGGCGAAA‑AACAAACCACCCGATTTTa‑aaaaaaaaaacaaaaaacgta > 2:507881/1‑225 (MQ=255) tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGGCGC‑GAG‑AAAACACTT‑CTGGC‑GGGAGAGA‑TTAAACT‑GGGGCC‑G‑AAAGA‑ATTTTCTG‑GGGCGC‑GAAA‑CTTTCCGGGGGAAA‑AACCACCCTCCCGATTTAA‑AAAAAAAcaaaaaaaggggt > 2:307294/1‑227 (MQ=255) atgatACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGCTGAGTTGTTTAACCCACTACCGC‑AAGAACTGAACGATCGCCAGCGGCCC‑GGG‑AAAACACTG‑CTTGA‑CGTAGAGA‑TTTAAAT‑GGCGCA‑T‑AAAAA‑TTTTCCTG‑GCGGGC‑AAAA‑C‑CTTCCGGGGCAA‑AACAAAACCGCACATTTAT‑AACAAAAA‑Caaccaaccctcatc > 2:902999/1‑226 (MQ=255) atcccgggttCAACTGGCATTTCCCCGTCTTTCGTGCACTGGTGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTACCCAGCTCCCGC‑AAGAACTGAACGGTGCTCAGCGCCGG‑GAG‑AAAACACTG‑CTGGC‑GGTGGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTgg < 2:941356/230‑1 (MQ=255) atACAAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGGTAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACCCTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTgg < 1:517086/239‑1 (MQ=255) cAATTTCTCGCCATTCGGGCACGGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATg < 1:546459/238‑1 (MQ=255) cAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTAGC‑GAG‑AAAAAACTG‑CTGGC‑GGGAGAGG‑ATAAAAC‑GGGGCC‑G‑AAAAA‑ATTTTCTG‑GGCCGC‑CAAA‑A‑TTTCCGGTGAAA‑ATAACCACACCCGCTTTTGAAACAAAAC‑AAACAGCGGGGTTACTGC‑GAGC‑Caaaaaaacaga > 2:2477/1‑233 (MQ=255) cctttCGGGCACTGGTGAAAGAAGTTAAAACCGGGCAGGCGGTTGAGTTTTTGAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAgggg < 1:889768/236‑1 (MQ=255) cATTCGGGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAgggg < 1:507881/238‑1 (MQ=255) aTTCGGGCACTGGGGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCg < 1:632276/239‑1 (MQ=255) cgctggcaaaaGAAGGGAAACCCGGTCCGGGTGTGGAGTGTTTTAACCAACGAACGA‑GAGAACTGAACGATGCTAAGCGTCGC‑GAG‑AAAACACGG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGGGGA‑A‑AAAGT‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGAGAAA‑ACCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTc < 2:666760/230‑1 (MQ=255) cACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTTTTTAACCAACTACCGC‑AAGAACTGAACGATGCTAAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTc < 1:2477/238‑1 (MQ=255) ccTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACTCTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa < 1:490844/238‑1 (MQ=255) aCTGGTGAAAGAAGGTAAAACGGGGAGGGCGGTTGGGTTGTTTAACCAAC‑GCCGCGAAGAACTGAACGAGGGTCAGGGTCGG‑GAA‑AAAACACTG‑CTGGG‑GGTAGGGA‑TTAAACT‑GGCGCA‑G‑AAAGG‑TTTTGCTG‑GCGCGA‑AAAA‑C‑TTGCTGGAGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa < 2:903650/239‑1 (MQ=255) gtgtgaaaaGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACCCTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑TTCACCCCTCCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa < 1:117892/232‑1 (MQ=255) cTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGGAA‑ATCCCACCTGCCGATTTTG‑AACAAAAC‑CAGCAAACGCGGTACTGG‑CAGG‑GAAAAAATCATGC‑CAGCCCAGGGGAG‑‑CCTTcc > 1:279691/1‑237 (MQ=255) cTGGGGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa < 1:718365/238‑1 (MQ=255) gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAACGCGTTACTGG‑CAGG‑CAAAAAACCACGC‑CAG‑CCCGGGGCG‑GCCTTCCATTgg > 1:93622/1‑238 (MQ=255) gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAACAACTACCGC‑CAGAACTGAACGATGCTCAGCGTCCC‑GAG‑AAAACACTG‑CTGGC‑GGCAGAGA‑TTAAACA‑GGCGCA‑G‑AAAGA‑TTTTTCTG‑GCCCGG‑CAAA‑C‑TTGCTTGGGAAA‑AACACACCCGCCGATTTAT‑AACAAAACAAAGCAA‑CGCGTTACTGGTCGGG‑CAAAAAACCATGC‑CAC‑CCAGAGGGG‑CCCTCCCAtat > 2:810673/1‑237 (MQ=255) aggaagGTAAAGCCGGCCAGGCGGTTGAGTTGGTTAACTAACTACCGC‑AAGAACTGAACGATGCTCAGCTACGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGATA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTa < 1:442636/237‑1 (MQ=255) aagGTAAACCCGGGGCGGGGGTTGGTTTGTTAAACCAACTACCGA‑AAAAATGGAAGGATCGCCAGGGGCGG‑GAG‑AAAACCTTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTg < 2:279691/239‑1 (MQ=255) ggTAAACCGGGGCGGGGGGTTGAGTTGTTTAACCAACGACCGA‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CGGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTg < 2:449168/237‑1 (MQ=255) tAAACCCGGGGAGGCGTTTGGTTTTTTTAACCAACTCCCGA‑AAGAACTGAACGGTGCCAAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTgcg < 2:136246/237‑1 (MQ=255) tAAACCCGGGCAGGGGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACGGAACGATGCCCAGCGTCGG‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTgcg < 1:683997/237‑1 (MQ=255) cGGGAAGGGGGTTGATTTTTTTAACCAACTACCGG‑AAGAACTGAACGATGCGCAGGGTCGC‑GAG‑AAAACACTG‑CGGGG‑GGTAGAGA‑TTAAACT‑GGCCAA‑G‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTaa < 2:208543/239‑1 (MQ=255) gggCAGGCCGGTGAGTTGGTTAACCAACGACCGA‑AAGAACTGAACGATGCTCAGCTACGC‑GAG‑AAAACACTG‑CTGGG‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGGGAAA‑ATCACCCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAAt < 1:573234/239‑1 (MQ=255) gggAAGGGGGTTGAGTTGTTTAACCAACTCCCGA‑AAGAACTGAACGATGCCCAGCGTCGG‑GAG‑AAAACCCTG‑CCGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACCCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAAt < 1:932090/239‑1 (MQ=255) gcggCGTTTGGTTTTTTTAACCAACTACCGA‑AAGAATTGAACGATTCTCAG‑GTTGCGGGG‑AAAACCCTG‑GTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTg < 2:769692/236‑1 (MQ=255) ggggggTTGAGTTGTTTACCCAACTCCCGC‑AATAAATGAACGATGCTCAGGGTCGC‑GAG‑AAACCACTG‑CTGGG‑GGTAGAGA‑TTAAACT‑GGCGAA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCt < 2:795727/235‑1 (MQ=255) cGGTTGAGTTTTTTAACCAACTACCGC‑AAGAACGGAACGATGCTTAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCa < 1:115751/238‑1 (MQ=255) gTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAgg < 1:661281/238‑1 (MQ=255) aGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAA‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCg < 1:350233/239‑1 (MQ=255) ttAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑CAAA‑C‑TTGCTGGCGAAA‑ATCCCACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑ACGG‑CCAAAAACGATGC‑CCA‑CCAAGGGCG‑CCCCTCCCTTGGTTTA‑CTGCCCGCGTTAAATTCTCAGGAAACgcagcat > 2:483104/1‑237 (MQ=255) tAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑GCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCCGGAACCGCGGCttt > 1:135195/1‑238 (MQ=255) aCTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTCCTGG‑CAGG‑CAAAAAACGATGCACAC‑CCAGGGG‑G‑GCCTTCCATTGGTTTA‑CTGGGCCCGTTAATTTCCCAGGAACCGCCGCTTGGGGCga > 2:865454/1‑239 (MQ=255) cTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CCG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTTCCCCGGAACCGCTGCTTGGGGCgaa > 1:547100/1‑239 (MQ=255) tACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATTC‑CAG‑CCAGGGGCG‑TCCTTCCa > 2:96670/1‑189 (MQ=255) tACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa < 1:96670/189‑1 (MQ=255) tACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAAACGATGC‑CAG‑CCAGGGGGG‑GCCTTCCATTGATTTA‑ATTGGCGCGGTAAATGCCCCGGAAACGCCGCTTGGGGCgaaa > 2:751801/1‑239 (MQ=255) gC‑AAGAACTGAACGATGCTCGGCGAGGG‑GAA‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCAC‑AAAA‑C‑TTGCTGGCGAAA‑ACCCACCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaagaaa < 2:93622/239‑1 (MQ=255) gC‑AAGAACTGAACGATGCCCAGCGTCGC‑GAG‑AAAACACCG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaagaaa < 1:186998/239‑1 (MQ=255) c‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCG‑AA‑ATCACACCTGCCGCTTTAA‑AACAAACA‑CAGCAAGCCCGTTACTGG‑CCGG‑CCAAAATCCATTC‑CCA‑CCAGGGGGG‑GCCTTCCATTCATTTA‑CCGCGCGCGGTTAATGCCCAGGGAACCCCGCTTTGGGCGAaaaaaaaa > 2:562027/1‑239 (MQ=255) aattgaacatgggcacgggTCGC‑GAA‑AAACCCCTT‑CGGGC‑GGTAAAGA‑TTAACCT‑GGCGCA‑G‑AAAGA‑TTTGGCTG‑CACCGC‑AAAC‑C‑TGTCTGGGGAAATACCAC‑CCTGCCGATGTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag < 2:467890/221‑1 (MQ=255) aaCTGAACGTTGCCCAGGGTCGG‑GAG‑AAACCCCTG‑CTGGC‑GGTAGAAA‑TTAAACT‑GGCGCA‑G‑AAAGT‑TTTTGCGG‑GCGCGA‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaag < 2:136147/231‑1 (MQ=255) aCTGAACGATGCTCAGCGTCGC‑GAG‑AAAACCCTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag < 1:638924/238‑1 (MQ=255) aCTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACCCCTGCCGGTTTAT‑AACAAAAC‑CACCAAGCGAGTTACTGG‑CAGG‑CAAAAAACCAGGC‑CCA‑CACGGGGCG‑CCCTTCCCTTGATTTAGCTGC‑CGCGATACTTGCCCAGGCACCCCCGGTTCGCCGGAaaaaaaaaaacg > 2:745209/1‑235 (MQ=255) tGAACGATGCTCAGGGTCGC‑GAG‑AAAACCCTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGAGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag < 1:975119/239‑1 (MQ=255) tGAACGATGCTCAGCGTCGC‑GAG‑AAAACCCTG‑GTGGC‑GGAAAAGA‑TTAAACG‑GGCGCA‑A‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag < 1:640064/239‑1 (MQ=255) tGAACGATGCTCAGCGTCGC‑GAG‑AAAACACGG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag < 1:265064/239‑1 (MQ=255) aaCGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCCGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGaa > 1:281803/1‑239 (MQ=255) gttggcaagggTCGG‑GAG‑AAAACCCTG‑CGGGG‑GGTAGGGA‑TTAAACG‑GGCGCA‑G‑AAAGT‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑AGCACCCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAatat < 2:829315/229‑1 (MQ=255) tGCTCAGCGTCGC‑GAG‑AAAACCCTG‑CTGGC‑GGTAGAAA‑TTAAACT‑GGCGAA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa < 1:397352/226‑1 (MQ=255) tGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGGCCAAAAACAGAT‑C‑CAA‑CCCGGGGCGCCCCTCCCATTG‑TTTT‑CCGCCCGGGTTAATTGGTCAGGAAACCCCCCTTGGCGCGGaaaaaaaaaaacaaaaaatat > 2:524285/1‑226 (MQ=255) tGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCCGGCGTTAATTGCTCAGGAACCGCTGCTTGGGCCGAAAGAAAAAaagcagctggt > 1:136147/1‑232 (MQ=255) tGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑GCCTTCCATTGGTTTA‑CTGCGCGCGTTAATTGCCCAGGAACCGCTGCTTTGCGCGAAAGAAAAACAACAGAATAttt > 1:745517/1‑238 (MQ=255) cAGCGTCGC‑GAG‑AAACCCCTG‑CGGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGc < 1:865454/239‑1 (MQ=255) cgc‑gAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAAG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCCTTGCTTTA‑CTGCCCCCGGTAATTTCTCCGGAACCGCTCCTTGGCGCGCAAGAAAAACACCaaaaaaacttgcccttata > 2:397352/1‑224 (MQ=255) gtccggaaaaaccccggtggggggTAGAAG‑TTAAACG‑GGGCAA‑A‑AAAGA‑TTTTGCTG‑GCCCGC‑AAAA‑C‑TTGATGGGAAAA‑ATCCCCCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCAc < 2:547100/216‑1 (MQ=255) aaaaaCCCTG‑CTGGC‑GGTAGAGA‑TTAAACG‑GGCGGA‑G‑AAAGT‑TTTTGGTG‑GCGCGA‑AAAC‑C‑TTGCTGGAGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAg < 1:51615/237‑1 (MQ=255) aacccgggtgggC‑GGTAGAGA‑TTAACTG‑GGCGCA‑G‑AAAGT‑TTTTGCTG‑GCGCGA‑AAAG‑C‑TTGCGGGAGAAA‑TCCACCCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGgcg < 2:62910/229‑1 (MQ=255) actctg‑ctgGC‑GGTGAAGA‑TTAAATT‑GGGCAA‑A‑AAAAA‑TTTTGCGG‑GC‑CGC‑AAAACC‑TTGGCGGCAAAA‑ACCACCCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGct < 2:709097/236‑1 (MQ=255) accctgtgggC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCTCGC‑AAAA‑C‑TTGCTGGGGAAA‑ATCACCCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGct < 1:1012454/231‑1 (MQ=255) cccttgtgGG‑GGTAGGGA‑TTAAACC‑GGGCAA‑A‑AAAGT‑TTTTGCTG‑G‑GCACAAAAA‑C‑TTTCTGGGGAAA‑ATCCACCCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTCAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc < 2:437955/233‑1 (MQ=255) gggaaacga‑TTAAATT‑GGACCA‑G‑AAGAA‑GTGTGAGG‑GCGCGA‑AAAA‑C‑TTGCTGGCGAAA‑ATCCCACCGGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACGGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAt < 1:1002598/231‑1 (MQ=255) gcgggagga‑TTAAACTCGGCCCA‑A‑AAAGA‑TGTTGCGG‑GCCCGA‑AAAA‑C‑TTGCCGGAGAAA‑ATCACACCGGACGAGTTAG‑AACAAAAC‑CAGCAAGCGCGTTACGGG‑CAGG‑CAAAAATCGATGC‑CAG‑CAAGGGGCG‑TCCTTCAATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAt < 2:195385/232‑1 (MQ=255) ttAAACT‑GGCGGA‑G‑AAATT‑TTTTGCTG‑CCGCAC‑AAAA‑C‑TGGCGGGAGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAAGCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGaa < 1:921737/239‑1 (MQ=255) gcgcA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa > 1:918409/1‑124 (MQ=255) gcgcA‑A‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa < 2:918409/124‑1 (MQ=255) cgcA‑A‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAACC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCACGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaa < 1:88965/238‑1 (MQ=255) | TCCACTGCTTATATGCAGGGCACGGCGCAGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGC‑GAG‑AAAACACTG‑CTGGC‑GGTAGAGA‑TTAAACT‑GGCGCA‑G‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑C‑TTGCTGGCGAAA‑ATCACACCTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAA > NC_000913/3293500‑3293966 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |