Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,293,775 A→C 14.1% T126T (ACA→ACC)  lpoA → OM lipoprotein stimulator of MrcA transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,293,7750AC14.1% 212.9 / 3.4 108T126T (ACA→ACC) lpoAOM lipoprotein stimulator of MrcA transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (26/59);  new base C (3/19);  total (29/79)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTCTGCAAGG  >  NC_000913/3293540‑3294005
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCCCGGGGAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCCG‑AAAGA‑TTTTGCTGTGCG‑GC‑AAAA‑CTTTCTGGCGAAA‑ATCA‑C‑Ac‑c                                                                                                                                                                                                                                              >  1:824564/1‑238 (MQ=255)
  tttttttttagagagatgagcaaaaGGTCTGATGTTACCAGGATCAACGGGAAATTACCGGCAATTCGGGCCCTGGTAAAAGAAGGTAAAACGGGGAAGGCGGTTGAGTTGTTTAACAAACTACCGA‑AAGAACTGAACGAGGCTCAGCGCGGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGc                                                                                                                                                                                                                                           <  2:395778/217‑1 (MQ=255)
  tttttacTTCAGCAGATGCAGCAAACCTCTGATGATACCAGGATCAACTGGCAATTCCTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGc                                                                                                                                                                                                                                           <  1:429913/233‑1 (MQ=255)
  ttttATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGc                                                                                                                                                                                                                                           <  1:551584/239‑1 (MQ=255)
        cTTCCGCAGATGCAGGAAAGCTCTGATGAATCCAGGATCAACTGGCAATTACCCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGAttt                                                                                                                                                                                                                                     <  1:214790/239‑1 (MQ=255)
          tCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAACAACTACCGC‑AAGAACTGAACGATGCCCAGCGTCGCGAGAAAAAACTGCCGGGGGGTAGAG‑TTTAACT‑TGGGCAC‑AAAAATTTTTTTTG‑GCGCGC‑AAAA‑CTTTGTGGGAAAA‑AACA‑c‑cc‑cccccgttata                                                                                                                                                                                                                                    >  2:683997/1‑229 (MQ=255)
                 aTGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTTTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGGTTAAAAT‑GGCGCGG‑AAAGA‑TTTTTCAG‑GCGCGC‑CAAA‑CTTGCTGGCGCAA‑ATCA‑C‑AA‑CTGCCGATTTTG‑Aaaaaaa                                                                                                                                                                                                                           >  1:437955/1‑239 (MQ=255)
                   gcagcaAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAACAACTACCGC‑AAGAACTGAAAGGTGCCCAGCGGCCGGGGAAAACAACG‑CTTGCGGTAGAGATTAAACA‑GGGGCCGTAAAGA‑TTTT‑TTT‑GCGCGC‑CAAA‑CATTCTGGCGGAA‑ATCA‑C‑AC‑CCCCCCCATTAG‑Aaaaaaaaa                                                                                                                                                                                                                         >  2:718365/1‑237 (MQ=255)
                         aagttttgatagtcccgggATGAAGTGGCAATTACGCGCCATTCGTTCACTGGTAAAAGAAGGTAAACCCGGGCAGGCGGTGGGGTTGTTTAACCAACTCCCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAg                                                                                                                                                                                                                  <  2:764984/222‑1 (MQ=255)
                         aagccttgatgaTACCAGGATCAACTGGCAATTACTCGCAATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAg                                                                                                                                                                                                                  <  1:227046/233‑1 (MQ=255)
                               tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTTTTTAACCAACTACCGC‑AAGAACTGAACCATGCTCAGCGTCGCGGGGAAACACTG‑CTGGCGGAAGAGATTTAACT‑TGCGCAG‑AAAAG‑ATTTGCGG‑GCGCGC‑CAAA‑CCTGTTGGGAAAA‑ATAA‑C‑AC‑CCGCCGATTTAG‑AAACAAAA‑CAACAAgaggagt                                                                                                                                                                                                            >  2:640064/1‑233 (MQ=255)
                               tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAAAACTG‑CTGGCGGTAGAGATTAAAAC‑TGCGCAG‑AAAAA‑TTTTTCTG‑GGGCCC‑AAAA‑ACTTCTGGCGAAA‑AACA‑A‑AC‑CACCCGATTTTa‑aaaaaaaaaacaaaaaacgta                                                                                                                                                                                                             >  2:507881/1‑225 (MQ=255)
                                 atgatACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGCTGAGTTGTTTAACCCACTACCGC‑AAGAACTGAACGATCGCCAGCGGCCCGGGAAAACACTG‑CTTGACGTAGAGATTTAAAT‑GGCGCAT‑AAAAA‑TTTTCCTG‑GCGGGC‑AAAA‑CCTTCCGGGGCAA‑AACA‑A‑AA‑CCGCACATTTAT‑AACAAAAA‑Caaccaaccctcatc                                                                                                                                                                                                          >  2:902999/1‑226 (MQ=255)
                                    atcccgggttCAACTGGCATTTCCCCGTCTTTCGTGCACTGGTGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTACCCAGCTCCCGC‑AAGAACTGAACGGTGCTCAGCGCCGGGAGAAAACACTG‑CTGGCGGTGGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTgg                                                                                                                                                                                                       <  2:941356/230‑1 (MQ=255)
                                    atACAAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGGTAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGGTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTgg                                                                                                                                                                                                       <  1:517086/239‑1 (MQ=255)
                                                    gCAATTACGGGCCTTTAGGGCACTGGGGAAAAAAGGTAAAACCGGGCAGGCGGTTAAGGTGTTAAACCAACAACCGA‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGGAGAGATTAAACG‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ACCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATg                                                                                                                                                                                     <  1:485199/239‑1 (MQ=255)
                                                     cAATTTCTCGCCATTCGGGCACGGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATg                                                                                                                                                                                     <  1:546459/238‑1 (MQ=255)
                                                     cAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTAGCGAGAAAAAACTG‑CTGGCGGGAGAGGATAAAAC‑GGGGCCG‑AAAAA‑ATTTTCTG‑GGCCGC‑CAAA‑ATTTCCGGTGAAA‑ATAA‑C‑CA‑CACCCGCTTTTGAAACAAAAC‑AAACAGCGGGGTTACTGC‑GAGC‑Caaaaaaacaga                                                                                                                                                                                     >  2:2477/1‑233 (MQ=255)
                                                          tctgcccattgtggCACTGGTGAAAGAAGGTAAACCCGGGCAGGGGGTTGTGTTGTTTAACCAACTACCGC‑AAGAACGAAACGATGCTCGGGGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTGGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATgc‑cag‑cc                                                                                                                                                                             <  2:512942/226‑1 (MQ=255)
                                                               cctttCGGGCACTGGTGAAAGAAGTTAAAACCGGGCAGGCGGTTGAGTTTTTGAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAgggg                                                                                                                                                                        <  1:889768/236‑1 (MQ=255)
                                                                cATTCGGGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAgggg                                                                                                                                                                        <  1:507881/238‑1 (MQ=255)
                                                                 aTTCGGGCACTGGGGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCg                                                                                                                                                                      <  1:632276/239‑1 (MQ=255)
                                                                        cgctggcaaaaGAAGGGAAACCCGGTCCGGGTGTGGAGTGTTTTAACCAACGAACGA‑GAGAACTGAACGATGCTAAGCGTCGCGAGAAAACACGG‑CTGGCGGTAGAGATTAAACT‑GGGGGAA‑AAAGT‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGAGAAA‑ACCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTc                                                                                                                                                               <  2:666760/230‑1 (MQ=255)
                                                                         ccTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACTCTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             <  1:490844/238‑1 (MQ=255)
                                                                         aCTGGTGAAAGAAGGTAAAACGGGGAGGGCGGTTGGGTTGTTTAACCAAC‑GCCGCGAAGAACTGAACGAGGGTCAGGGTCGGGAAAAAACACTG‑CTGGGGGTAGGGATTAAACT‑GGCGCAG‑AAAGG‑TTTTGCTG‑GCGCGA‑AAAA‑CTTGCTGGAGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             <  2:903650/239‑1 (MQ=255)
                                                                         aCTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAAAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             <  1:905569/239‑1 (MQ=255)
                                                                          gtgtgaaaaGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACCCTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑TTCA‑C‑CC‑CTCCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             <  1:117892/232‑1 (MQ=255)
                                                                          cTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGGAA‑ATCC‑C‑AC‑CTGCCGATTTTG‑AACAAAAC‑CAGCAAACGCGGTACTGG‑CAGG‑GAAAAAATCATGC‑CAGCCCAGGGGAG‑‑CCTTcc                                                                                                                                                              >  1:279691/1‑237 (MQ=255)
                                                                          cTGGGGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             <  1:718365/238‑1 (MQ=255)
                                                                             gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAACGCGTTACTGG‑CAGG‑CAAAAAACCACGC‑CAG‑CCCGGGGCG‑GCCTTCCATTgg                                                                                                                                                         >  1:93622/1‑238 (MQ=255)
                                                                             gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAAACAACTACCGC‑CAGAACTGAACGATGCTCAGCGTCCCGAGAAAACACTG‑CTGGCGGCAGAGATTAAACA‑GGCGCAG‑AAAGA‑TTTTTCTG‑GCCCGG‑CAAA‑CTTGCTTGGGAAA‑AACA‑C‑AC‑CCGCCGATTTAT‑AACAAAACAAAGCAA‑CGCGTTACTGGTCGGG‑CAAAAAACCATGC‑CAC‑CCAGAGGGG‑CCCTCCCAtat                                                                                                                                                          >  2:810673/1‑237 (MQ=255)
                                                                                 aggaagGTAAAGCCGGCCAGGCGGTTGAGTTGGTTAACTAACTACCGC‑AAGAACTGAACGATGCTCAGCTACGCGAGAAAACACTG‑CTGGCGGTAGATATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTa                                                                                                                                                     <  1:442636/237‑1 (MQ=255)
                                                                                    aagGTAAACCCGGGGCGGGGGTTGGTTTGTTAAACCAACTACCGA‑AAAAATGGAAGGATCGCCAGGGGCGGGAGAAAACCTTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTg                                                                                                                                                 <  2:279691/239‑1 (MQ=255)
                                                                                      ggTAAACCGGGGCGGGGGGTTGAGTTGTTTAACCAACGACCGA‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CGGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTg                                                                                                                                                 <  2:449168/237‑1 (MQ=255)
                                                                                        tAAAGCCGGGAAGGCGGTGGAGTTGTTTAACCAACGACCGG‑AAGAACTGAACGATGCTCAGCGTTGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTGTGCTG‑GCGCGA‑AAAC‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTgcg                                                                                                                                               <  1:249360/237‑1 (MQ=255)
                                                                                        tAAACCCGGGGAGGCGTTTGGTTTTTTTAACCAACTCCCGA‑AAGAACTGAACGGTGCCAAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTgcg                                                                                                                                               <  2:136246/237‑1 (MQ=255)
                                                                                        tAAACCCGGGCAGGGGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACGGAACGATGCCCAGCGTCGGGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTgcg                                                                                                                                               <  1:683997/237‑1 (MQ=255)
                                                                                              cGGGAAGGGGGTTGATTTTTTTAACCAACTACCGG‑AAGAACTGAACGATGCGCAGGGTCGCGAGAAAACACTG‑CGGGGGGTAGAGATTAAACT‑GGCCAAG‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTaa                                                                                                                                       <  2:208543/239‑1 (MQ=255)
                                                                                               gggAAGGGGGTTGAGTTGTTTAACCAACTCCCGA‑AAGAACTGAACGATGCCCAGCGTCGGGAGAAAACCCTG‑CCGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAAt                                                                                                                                      <  1:932090/239‑1 (MQ=255)
                                                                                                ggCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGGAGAGATTAAACT‑GGCGCCG‑GAAAA‑TTTTTCTG‑GCACGC‑AAAA‑CCTGCTGGCGAAA‑ATCA‑C‑ACACTCCCGTTTTGA‑AAAAAACC‑CCACAACGCCGTTACTGG‑AAGG‑AAAAAATCTGTG‑‑CAG‑CCAGGGGCG‑CCCTCCCACTTATTTA‑TCGcgggcggtgaag                                                                                                                                     >  2:899821/1‑233 (MQ=255)
                                                                                                   ggggggTTGAGTTGTTTACCCAACTCCCGC‑AATAAATGAACGATGCTCAGGGTCGCGAGAAACCACTG‑CTGGGGGTAGAGATTAAACT‑GGCGAAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCt                                                                                                                                  <  2:795727/235‑1 (MQ=255)
                                                                                                      cGGTTGAGTTTTTTAACCAACTACCGC‑AAGAACGGAACGATGCTTAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCa                                                                                                                                <  1:115751/238‑1 (MQ=255)
                                                                                                        gTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAgg                                                                                                                              <  1:661281/238‑1 (MQ=255)
                                                                                                            aGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAAAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCg                                                                                                                         <  1:350233/239‑1 (MQ=255)
                                                                                                                  ttAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑CAAA‑CTTGCTGGCGAAA‑ATCC‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑ACGG‑CCAAAAACGATGC‑CCA‑CCAAGGGCG‑CCCCTCCCTTGGTTTA‑CTGCCCGCGTTAAATTCTCAGGAAACgcagcat                                                                                                                   >  2:483104/1‑237 (MQ=255)
                                                                                                                   tAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑GCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCCGGAACCGCGGCttt                                                                                                                  >  1:135195/1‑238 (MQ=255)
                                                                                                                         aCTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTCCTGG‑CAGG‑CAAAAAACGATGCACAC‑CCAGGGG‑G‑GCCTTCCATTGGTTTA‑CTGGGCCCGTTAATTTCCCAGGAACCGCCGCTTGGGGCga                                                                                                            >  2:865454/1‑239 (MQ=255)
                                                                                                                          cTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CCG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTTCCCCGGAACCGCTGCTTGGGGCgaa                                                                                                           >  1:547100/1‑239 (MQ=255)
                                                                                                                           tACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATTC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             >  2:96670/1‑189 (MQ=255)
                                                                                                                           tACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             <  1:96670/189‑1 (MQ=255)
                                                                                                                           tACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAC‑CCCGGGGCG‑CCCTTCCCTTGATTTA‑TTGCGCGCGGTAAATGCCCAGGAACCGCGGCTTGgcggggaa                                                                                                          >  2:1004039/1‑234 (MQ=255)
                                                                                                                           tACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAAACGATGC‑CAG‑CCAGGGGGG‑GCCTTCCATTGATTTA‑ATTGGCGCGGTAAATGCCCCGGAAACGCCGCTTGGGGCgaaa                                                                                                          >  2:751801/1‑239 (MQ=255)
                                                                                                                               gC‑AAGAACTGAACGATGCTCGGCGAGGGGAAAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCAC‑AAAA‑CTTGCTGGCGAAA‑ACCC‑A‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaagaaa                                                                                                      <  2:93622/239‑1 (MQ=255)
                                                                                                                               gC‑AAGAACTGAACGATGCCCAGCGTCGCGAGAAAACACCG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaagaaa                                                                                                      <  1:186998/239‑1 (MQ=255)
                                                                                                                                c‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCG‑AA‑ATCA‑C‑AC‑CTGCCGCTTTAA‑AACAAACA‑CAGCAAGCCCGTTACTGG‑CCGG‑CCAAAATCCATTC‑CCA‑CCAGGGGGG‑GCCTTCCATTCATTTA‑CCGCGCGCGGTTAATGCCCAGGGAACCCCGCTTTGGGCGAaaaaaaaa                                                                                                    >  2:562027/1‑239 (MQ=255)
                                                                                                                                     aattgaacatgggcacgggTCGCGAAAAACCCCTT‑CGGGCGGTAAAGATTAACCT‑GGCGCAG‑AAAGA‑TTTGGCTG‑CACCGC‑AAAC‑CTGTCTGGGGAAATACCA‑C‑‑C‑CTGCCGATGTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag                                                                                                 <  2:467890/221‑1 (MQ=255)
                                                                                                                                     aaCTGAACGTTGCCCAGGGTCGGGAGAAACCCCTG‑CTGGCGGTAGAAATTAAACT‑GGCGCAG‑AAAGT‑TTTTGCGG‑GCGCGA‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaaag                                                                                                         <  2:136147/231‑1 (MQ=255)
                                                                                                                                      aCTGAACGATGCTCAGCGTCGCGAGAAAACCCTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag                                                                                                 <  1:638924/238‑1 (MQ=255)
                                                                                                                                      aCTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑CC‑CTGCCGGTTTAT‑AACAAAAC‑CACCAAGCGAGTTACTGG‑CAGG‑CAAAAAACCAGGC‑CCA‑CACGGGGCG‑CCCTTCCCTTGATTTAGCTGC‑CGCGATACTTGCCCAGGCACCCCCGGTTCGCCGGAaaaaaaaaaacg                                                                                                >  2:745209/1‑235 (MQ=255)
                                                                                                                                        tGAACGATGCTCAGCGTCGCGAGAAAACCCTG‑GTGGCGGAAAAGATTAAACG‑GGCGCAA‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag                                                                                              <  1:640064/239‑1 (MQ=255)
                                                                                                                                        tGAACGATGCTCAGCGTCGCGAGAAAACACGG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcagcag                                                                                              <  1:265064/239‑1 (MQ=255)
                                                                                                                                          aaCGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCCGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGaa                                                                                            >  1:281803/1‑239 (MQ=255)
                                                                                                                                             gttggcaagggTCGGGAGAAAACCCTG‑CGGGGGGTAGGGATTAAACG‑GGCGCAG‑AAAGT‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑AGCA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAatat                                                                                         <  2:829315/229‑1 (MQ=255)
                                                                                                                                               tGCTCAGCGTCGCGAGAAAACCCTG‑CTGGCGGTAGAAATTAAACT‑GGCGAAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa                                                                                                    <  1:397352/226‑1 (MQ=255)
                                                                                                                                               tGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGGCCAAAAACAGAT‑C‑CAA‑CCCGGGGCGCCCCTCCCATTG‑TTTT‑CCGCCCGGGTTAATTGGTCAGGAAACCCCCCTTGGCGCGGaaaaaaaaaaacaaaaaatat                                                                                       >  2:524285/1‑226 (MQ=255)
                                                                                                                                               tGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCCGGCGTTAATTGCTCAGGAACCGCTGCTTGGGCCGAAAGAAAAAaagcagctggt                                                                                         >  1:136147/1‑232 (MQ=255)
                                                                                                                                               tGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑GCCTTCCATTGGTTTA‑CTGCGCGCGTTAATTGCCCAGGAACCGCTGCTTTGCGCGAAAGAAAAACAACAGAATAttt                                                                                       >  1:745517/1‑238 (MQ=255)
                                                                                                                                                   cAGCGTCGCGAGAAACCCCTG‑CGGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGc                                                                                   <  1:865454/239‑1 (MQ=255)
                                                                                                                                                        gtccggaaaaaccccggtggggggTAGAAGTTAAACG‑GGGCAAA‑AAAGA‑TTTTGCTG‑GCCCGC‑AAAA‑CTTGATGGGAAAA‑ATCC‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCAc                                                                                <  2:547100/216‑1 (MQ=255)
                                                                                                                                                         cgcgAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAAG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCCTTGCTTTA‑CTGCCCCCGGTAATTTCTCCGGAACCGCTCCTTGGCGCGCAAGAAAAACACCaaaaaaacttgcccttata                                                                             >  2:397352/1‑224 (MQ=255)
                                                                                                                                                              aaaaaCCCTG‑CTGGCGGTAGAGATTAAACG‑GGCGGAG‑AAAGT‑TTTTGGTG‑GCGCGA‑AAAC‑CTTGCTGGAGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAg                                                                         <  1:51615/237‑1 (MQ=255)
                                                                                                                                                                 aacccgggtgggCGGTAGAGATTAACTG‑GGCGCAG‑AAAGT‑TTTTGCTG‑GCGCGA‑AAAG‑CTTGCGGGAGAAA‑TCCA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGgcg                                                                      <  2:62910/229‑1 (MQ=255)
                                                                                                                                                                  actctg‑ctgGCGGTGAAGATTAAATT‑GGGCAAA‑AAAAA‑TTTTGCGG‑GC‑CGC‑AAAACCTTGGCGGCAAAA‑ACCA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGct                                                                    <  2:709097/236‑1 (MQ=255)
                                                                                                                                                                   accctgtgggCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCTCGC‑AAAA‑CTTGCTGGGGAAA‑ATCA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGct                                                                    <  1:1012454/231‑1 (MQ=255)
                                                                                                                                                                    cccttgtgGGGGTAGGGATTAAACC‑GGGCAAA‑AAAGT‑TTTTGCTG‑G‑GCACAAAAA‑CTTTCTGGGGAAA‑ATCC‑A‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTCAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc                                                                   <  2:437955/233‑1 (MQ=255)
                                                                                                                                                                    cccgtctgGCGGTAAGAATTAAACT‑GGCCAAG‑AAAGT‑TTTTGCTG‑GGGCGA‑AAAA‑CTTTGTGGGGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc                                                                   <  1:42616/234‑1 (MQ=255)
                                                                                                                                                                             gggaaacgaTTAAATT‑GGACCAG‑AAGAA‑GTGTGAGG‑GCGCGA‑AAAA‑CTTGCTGGCGAAA‑ATCC‑C‑AC‑CGGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACGGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAt                                                           <  1:1002598/231‑1 (MQ=255)
                                                                                                                                                                             gcgggaggaTTAAACTCGGCCCAA‑AAAGA‑TGTTGCGG‑GCCCGA‑AAAA‑CTTGCCGGAGAAA‑ATCA‑C‑AC‑CGGACGAGTTAG‑AACAAAAC‑CAGCAAGCGCGTTACGGG‑CAGG‑CAAAAATCGATGC‑CAG‑CAAGGGGCG‑TCCTTCAATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAt                                                           <  2:195385/232‑1 (MQ=255)
                                                                                                                                                                                      ttAAACT‑GGCGGAG‑AAATT‑TTTTGCTG‑CCGCAC‑AAAA‑CTGGCGGGAGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAAGCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGaa                                                 <  1:921737/239‑1 (MQ=255)
                                                                                                                                                                                       ttaaccgggccaaG‑AAAGT‑TTTTGCGG‑GCCCGC‑AAAA‑CTTGCTGGCGAAA‑ACCA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGaa                                                 <  1:473199/227‑1 (MQ=255)
                                                                                                                                                                                             tgggggaaaagggA‑TTTTATTG‑GCG‑GC‑AAAATCTTGCTGGGAAAA‑ATCG‑C‑CC‑CTGCCTGTTGCA‑ATCAAACT‑CAGGAGGGGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCAACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGc                                            <  2:660959/227‑1 (MQ=255)
                                                                                                                                                                                               gcgcAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             >  1:918409/1‑124 (MQ=255)
                                                                                                                                                                                               gcgcAA‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCa                                                                                                                                                             <  2:918409/124‑1 (MQ=255)
                                                                                                                                                                                                cgcAA‑AAAGA‑TTTTGCTG‑GCGCGA‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAACC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCACGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaa                                         <  1:88965/238‑1 (MQ=255)
                                                                                                                                                                                                  gataatata‑TTTTGCGG‑GCGCAA‑AAAA‑CTTGCGTTGAAAA‑ATCA‑C‑CC‑CTGCAGATTTAG‑AACAAAAC‑CGGCAAGCGGGTGACGGG‑CAGG‑CAAAAATCGAGGA‑CAG‑CCAGGGGCT‑TCCCTCCATGGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGAGGCCGCCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATa                                       <  2:184260/231‑1 (MQ=255)
                                                                                                                                                                                                   tacaaaGT‑TTTTCCTG‑GCTCGA‑AAAA‑TGTG‑TGGTGAAA‑AACA‑CAAC‑CTGCTGATTTAG‑AACAAAAC‑CAGTAAGGGGCTTCCTGG‑CCGG‑CAAAAATTGATGG‑CAG‑GC‑GGGGCGTTCCTGCCATGGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCAACCTGGCAGGCGTTCTCCTCCATGACTCAGGATCAGGCGAATa                                       <  2:767420/235‑1 (MQ=255)
                                                                                                                                                                                                    aaaaaGA‑GTTTGGGG‑GCGGGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑CC‑CTGCCGATTTAG‑ACCAAAAC‑CAGCAAGCGCGGTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCTCCTGGCAGGCGCTCTCCTCCATGACGCAGGAACAGGCGAATACg                                     <  1:1062241/237‑1 (MQ=255)
                                                                                                                                                                                                     caaaattttttGCGG‑GCCCGA‑AAAA‑CTTGCGGGGAAAA‑ATCC‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACg                                     <  2:135195/232‑1 (MQ=255)
                                                                                                                                                                                                      aaaattttttGCGG‑GCCCGA‑AAAA‑CTTGGCGGGGAAA‑CCCC‑C‑AC‑CTGGCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGc                                    <  2:281803/233‑1 (MQ=255)
                                                                                                                                                                                                      aaaGA‑TTTTGCGG‑GCGCGC‑AAAA‑CTTGCTGGCAAAA‑ATCC‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑C‑AAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTg                                  <  1:307294/239‑1 (MQ=255)
                                                                                                                                                                                                       aaGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCt                                   <  1:786410/238‑1 (MQ=255)
                                                                                                                                                                                                       aaGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCAAAA‑ATCA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACg                                     <  1:524285/236‑1 (MQ=255)
                                                                                                                                                                                                         aagttttTCTG‑GAGCCG‑AAAT‑CTTGGGGGCGAAA‑AACAGC‑AC‑CGTCCGATTAAG‑CACAAAAC‑CAGGAAGCGCGTCCCTTG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑GCCTTCCATTGATTTA‑CTGCGCGAGTGAATTGCGCAGGACCCGCTGCTTGGCGCGAAAGAAAAACAGCAGAAGATTGATGCCACCGGGCAGGCGCTCTCCTCCATGACTCAGGAACAGTCGAATACGCTg                                  <  2:174852/236‑1 (MQ=255)
                                                                                                                                                                                                                       gggc‑AAAA‑CTTGCTGGCGAAA‑A‑CA‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCcga                    <  1:562027/236‑1 (MQ=255)
                                                                                                                                                                                                                       gcga‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCcga                    <  1:1004039/239‑1 (MQ=255)
                                                                                                                                                                                                                         cgaaaaa‑CTTGCTGGCGAAA‑ATCC‑C‑CC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCcga                    <  1:595353/235‑1 (MQ=255)
                                                                                                                                                                                                                                       gggaaaa‑aACC‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTCTg       <  1:902999/234‑1 (MQ=255)
                                                                                                                                                                                                                                         cGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGa                                                         >  1:659806/1‑186 (MQ=255)
                                                                                                                                                                                                                                            aa‑aTCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTCTGCAAgg  <  1:226055/238‑1 (MQ=255)
                                                                                                                                                                                                                                            aa‑aTCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTCTGCAAgg  <  1:751801/238‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
CCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAACCAACTACCGC‑AAGAACTGAACGATGCTCAGCGTCGCGAGAAAACACTG‑CTGGCGGTAGAGATTAAACT‑GGCGCAG‑AAAGA‑TTTTGCTG‑GCGCGC‑AAAA‑CTTGCTGGCGAAA‑ATCA‑C‑AC‑CTGCCGATTTAG‑AACAAAAC‑CAGCAAGCGCGTTACTGG‑CAGG‑CAAAAATCGATGC‑CAG‑CCAGGGGCG‑TCCTTCCATTGATTTA‑CTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTCTGCAAGG  >  NC_000913/3293540‑3294005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑