Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,665,654 T→A 15.6% V52V (GTA→GTT gadX ← acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,6540TA15.6% 206.9 / 3.2 76V52V (GTA→GTTgadXacid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand):  ref base T (36/26);  new base A (3/10);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 3.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTA  >  NC_000913/3665419‑3665869
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          
aGTTCTTATCCTCAAGGAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACa                                                                                                                                                                                                                         <  1:284709/239‑1 (MQ=255)
    cTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAAAAAAAAt                                                                                                                                                                                                                     >  2:741969/1‑239 (MQ=255)
    cTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGAATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGAAACGAAAAAAAAAt                                                                                                                                                                                                                     >  2:611928/1‑239 (MQ=255)
     tCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAAtt                                                                                                                                                                                                                    >  1:802651/1‑239 (MQ=255)
       aTCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACCCGAATAAAAAAtttt                                                                                                                                                                                                                  >  2:454699/1‑239 (MQ=255)
       aTCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAAtttt                                                                                                                                                                                                                  >  1:680678/1‑239 (MQ=255)
         ccTTAAAAAAAACAGATAACCACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCa                                                                                                                                                                                                               <  2:220788/239‑1 (MQ=255)
          ctaaaGAAAAACGGATTACAACGCAAAGATTAATGCTTTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  2:202328/236‑1 (MQ=255)
          cTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  2:353468/239‑1 (MQ=255)
          cTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  1:950830/239‑1 (MQ=255)
          cTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  2:994064/239‑1 (MQ=255)
          cTCAAGAAAAACAGAAAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  2:941068/239‑1 (MQ=255)
            cAAAAAAAACAGATAACAACGCAAAGATTAATGCTCGTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAaca                                                                                                                                                                                                            <  1:41471/239‑1 (MQ=255)
            aaaaaaaaaaAGAGAACAACGAAAAGATTAATGCTTTTTTACGTAGCATGTCTGCATAGCGAAGATTCTTATTCGGCGATAGTTGCGCAAATTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAaca                                                                                                                                                                                                            <  1:393799/235‑1 (MQ=255)
                    aCAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATc                                                                                                                                                                                                    <  1:366399/239‑1 (MQ=255)
                       gATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGCATACAAAATTTTCAACACACTTATCTa                                                                                                                                                                                                  >  1:862976/1‑238 (MQ=255)
                             aacGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGGGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAAt                                                                                                                                                                                           >  2:619435/1‑239 (MQ=255)
                              acGAAAAGATTTATTCTTTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAAtt                                                                                                                                                                                          <  2:17730/239‑1 (MQ=255)
                               cgaaaaGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  2:541675/215‑1 (MQ=255)
                               cGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              >  1:541675/1‑218 (MQ=255)
                               cGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAAttt                                                                                                                                                                                         >  1:834443/1‑239 (MQ=255)
                                  aaaGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTg                                                                                                                                                                                        >  1:412217/1‑237 (MQ=255)
                                         aaTGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                                                               >  1:874008/1‑238 (MQ=255)
                                            gCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGc                                                                                                                                                                           <  1:892092/239‑1 (MQ=255)
                                             cttttttTCGTTACATTTTTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                                          <  2:874008/236‑1 (MQ=255)
                                                ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACCCGAATTTGGCTTGCATCCCaaaaa                                                                                                                                                                      >  2:745493/1‑239 (MQ=255)
                                                             ttCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCa                                                                                                                                                                          <  1:933306/223‑1 (MQ=255)
                                                                    tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTaaa                                                                                                                                                  >  2:857306/1‑239 (MQ=255)
                                                                       cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATa                                                                                                                                               >  1:52086/1‑239 (MQ=255)
                                                                       cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCCCAAAAACCAGGACACCGCCCTTaaaaaa                                                                                                                                               >  2:427008/1‑237 (MQ=255)
                                                                       cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAATTTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGCCCCGAAAACAAACTTTCCAAAACAATTACCTACCCAAATTTGCCTTCCATCGCCAAAACCCAGGTccggtctcttaaaaac                                                                                                                                               >  2:933306/1‑224 (MQ=255)
                                                                          aGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGCCACGAATACAAAATTTTCAACACACTTATATACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAAAAGCg                                                                                                                                            >  2:988145/1‑239 (MQ=255)
                                                                                 tATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATATCGCTATTc                                                                                                                                      >  1:414666/1‑238 (MQ=255)
                                                                                    tCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAAAAGCGATATTCACCa                                                                                                                                  >  2:155512/1‑239 (MQ=255)
                                                                                      tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTAt                                                                                                                                                                                                     <  2:613264/172‑1 (MQ=255)
                                                                                      tGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTAt                                                                                                                                                                                                     >  1:613264/1‑172 (MQ=255)
                                                                                              ttGCGCACTTTCATAAAAACCAGTAATTTATCTACAAAAGTGAGAAACAATAAAGGAAGAAATTTTTTTATGTGCATGAAGATTTAAGGCCTCCTCCTGAACCACCGGGAAAAAAAATAAAAGCGTGTCCTTTGACACAAAAACAAAATTTTCAACACTCTTATTGACTGAATTTTGGCTGGCACCCGAAAAACCAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                                        <  1:92010/239‑1 (MQ=255)
                                                                                                gctcAATTTCCTAAAAAAGAGAAATCCCCATACTACAGTGTAGAACGATTAAAGAAGAATCTTTTTTAGGTGCAAGAATTTTCAATGCCCCCTCCTGGACCATCGGAAAAAATAATGAAGGCTTTGCCCTTGACACAAATACAAATTTTTCAACACTCTTATCTACTCGATTTTGGCTTCCTACCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                                                      <  1:611928/236‑1 (MQ=255)
                                                                                                    catctccaaaaaaacgaaaaTTTCTCTACAAGAGTGAAGAACGAGAAATGAAGAATTTTTTTTAGGTGAATAAAGATTTAATGCCCCCTCCGTGAACATGGGGAAAAATAATGAAAGGGTGTCCTTTGACAAAAAAAAAAATTTTTCAACACACTTTTCTCCTCGATTTTGGCTTGCATCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                  <  1:267698/221‑1 (MQ=255)
                                                                                                    aatttCTTGAAAAATAGGTATATTTTTTCTACAGTGATGAAGGATTAATAAAGAATCTTTTTTATGTGCATAAAGATTTAATGCCTCCTCATGGACAAGCGGAAAAAATAAGGAAGGCTTGTCCCTTGAAACAAATAAAAAATTTCAAACACACTTTCCTACTCAATTGTGTTTTGAACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                  <  2:412217/236‑1 (MQ=255)
                                                                                                      tttcataaaaaaCAGGAATATTTCAACTAAGGTGAGAAACGAGTAATGAAAAACTTTTTTATTGTCAAGTAAGTTTTAATCCCTCCACTTAGAGCATGGGAAAAAAAAATGAAGGCGTTTCCCTTGACAAAAAAAAAAATTTTTAAACCCTTTTATCTATTCAATTTTGGCTTGCCTCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAata                                                                                                                <  1:754531/232‑1 (MQ=255)
                                                                                                                    ggAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGGGAGAATATATTTATGTCTTGc                                                                                                  >  1:570064/1‑239 (MQ=255)
                                                                                                                       tttttcctctcaaggtaagaaaCGAGTAAAAAAAAACTTTGTTGTGTGGAATAAAATTTTATTCCCCCCTCCTGGAGCGCGGGAAAAAAAAATAAAGGCGGGCCCTTAGCCACAAAAACAAATTTTCAAACCCACTCTCTAACAAAATTTGGCTTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                               <  2:691667/219‑1 (MQ=255)
                                                                                                                       atctctaTACTAGAGTGAGAAACGAGAAATAAAAAAACTTTTTTAGGTGAATGAATTTTAAATGCCTCCCCCGGAAGCATGGGGAAAAATAATGAAAGCGTTCCCTTTAACAAAAAAAAAAATTTTCCAACACACTTATCTACTAGATTTTGGCTTGCATCCACAAAAACGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                               <  1:745493/236‑1 (MQ=255)
                                                                                                                             ctactaCAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAATACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAAt                                                                                         >  1:139364/1‑239 (MQ=255)
                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  2:129821/115‑1 (MQ=255)
                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              >  1:129821/1‑115 (MQ=255)
                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCAc                                                                                                                                    >  2:70631/1‑187 (MQ=255)
                                                                                                                                      tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                                         >  2:58603/1‑214 (MQ=255)
                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                       >  1:927945/1‑182 (MQ=255)
                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCCTTAACCATGGTGAGAatatat                                                                                                             >  1:519470/1‑208 (MQ=255)
                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                             >  1:586212/1‑208 (MQ=255)
                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                               >  1:277882/1‑238 (MQ=255)
                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCAAACGCAATTAAAAAATTc                                                                               >  2:764034/1‑238 (MQ=255)
                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCAATTAAAATAGCGATATTCACCa                                                                                                                                  >  1:242928/1‑187 (MQ=255)
                                                                                                                                        atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCACAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                               >  1:839073/1‑238 (MQ=255)
                                                                                                                                                      aagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              >  1:785756/1‑99 (MQ=255)
                                                                                                                                                      aagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCaa                                                                                                                                                                                                              <  2:785756/99‑1 (MQ=255)
                                                                                                                                                         aaaCTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATgaa                                                                        >  1:664166/1‑226 (MQ=255)
                                                                                                                                                           ttttttttATGTGCATAAATATTTAATCCCTCCTCGTGGAGCGTCGGAAAAAAAAAGAAAGTCGTGCCCTTTGAACCAAAAAAAAAATTTTAAACACACTTATCTACTCAATTTTGGTTGGCACCCGAAAAACCCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:973589/237‑1 (MQ=255)
                                                                                                                                                           ttttttttAGGTGCAAGAATTTTTAAGCCCTCCTCCTTGAGCACGGGAAAAAAAAATAAAAGCTTGCCCCTTGACACAAATAAAAAATTTACAACACACTTATCTACTAAATTTTGGCTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:145811/237‑1 (MQ=255)
                                                                                                                                                           ttttgtttATGTGCTTGAAGTTTTAATGCCCCGTCCTTGAGCAGCGGTAAAAATAATAAAAGCGTCGCCCTGAACACGAAATCAAATTTTACAACATACTTCTCTACACGAATTTGGCTACAATCCAAAAAACAGAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:59752/234‑1 (MQ=255)
                                                                                                                                                           aCTTTTTTATGAGAAGAAAGATTTAAGCCCTCCTCTATGAGCATGGGGAAAAAAAATGAATGCGTGTCCCTGGACACGAACAAAAAATTACCAACACACTAACCTACTAGAATTTGCTTGGCTCCCACAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                           <  1:521972/239‑1 (MQ=255)
                                                                                                                                                                       agaagatatgttttAATCCCTCCTCATTGAGCATCGGAAAAAATAAGAAATGCGTTTCCCTTGACACAAATAAAAAATTTCCAACACACTTACCTCCTCGATTTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                                                 <  2:307948/228‑1 (MQ=255)
                                                                                                                                                                         gaaTGAAGTTTAAAGCCCCCCTCCTTGATCACGGGGAAAATAAATGAAAGCGTGCCCTTTGCACCAAAAAAAAAATTTCCAACACCTTTATCTACCAGAATTTGGCTTGAATCAGAAAAACCCGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                                                <  1:857306/236‑1 (MQ=255)
                                                                                                                                                                             gaaaattaaaccccccctccgTAACCATGGGGAGAAATAATGAAAGCGGCCCTCTTGACAAGAATATAAATTCTCCAACCCCATTAGCTTCGCGAATTTCGCTTGCCCCCACAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:230515/224‑1 (MQ=255)
                                                                                                                                                                             aaatattaatcccccccccctTGAGCAGCGGAAAAAAAAAAGAAGGCTGTCCCCTTGACAAGAAAAAAAAATTCACAACACTTTTATCCACTCAATTTTGGCTTGCACCCGAAAAACCCGGTCCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:454699/221‑1 (MQ=255)
                                                                                                                                                                             aaagttttaacccccccactgtGAGCAAGGGGAAAAATAAAAAAACCGTGCCCCTTGACACAAATAAAAATTTTCCAACACTCTTATCTACTCGATTTTGGCTTCCACCCGAAAAACCCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:741969/218‑1 (MQ=255)
                                                                                                                                                                             aaaGATTTAATGCCTCCCCTTGAAGCAGCGGGAAAAATAATGAACGCGTGTCCCTTGACAAAAATACAAATTTTACAACCCATTTACTCACTCAATTTGGGCTCGCACCCACAAAACCAGGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                           <  1:764034/238‑1 (MQ=255)
                                                                                                                                                                                         cccccctcatttaagaTCGGGAAAAAATATGAAAAGGTTGTCCTTGACAAAAAAAAAAAATTTTCCACCACTTTTTTCAATTAGATTTTGTCTTCCACCAAAAAAAACAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTa                                 <  2:802651/224‑1 (MQ=255)
                                                                                                                                                                                           tcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAc                                                                                                                             >  1:487406/1‑143 (MQ=255)
                                                                                                                                                                                           tcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACCTAAAc                                       >  1:307948/1‑229 (MQ=255)
                                                                                                                                                                                           tcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAAAATTAAATAAACAAATTaattt                             >  2:358600/1‑235 (MQ=255)
                                                                                                                                                                                            cctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                             >  1:556043/1‑158 (MQ=255)
                                                                                                                                                                                                cctgaagaagcggaaaaaaaaaTAAAAGCGTCTCCTTGCACAAAAATAAAAATTTTACAACACTCTTATCTGCTAAATTTTGGCTGGCACACAAAAAACCAGGTCCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTttat                         <  1:437865/223‑1 (MQ=255)
                                                                                                                                                                                                                       gAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTa  >  1:234069/1‑239 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          
AGTGCTCATCCTCAAGAAAAACAGATAACAACGCAAAGATTAATGCTCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTA  >  NC_000913/3665419‑3665869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: