Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 421,546 G→T 18.7% L187L (CTG→CTT proY → proline‑specific permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913421,5460GT18.7% 112.7 / 3.0 64L187L (CTG→CTTproYproline‑specific permease
Reads supporting (aligned to +/- strand):  ref base G (23/28);  new base T (10/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 6.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01

TATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCG  >  NC_000913/421322‑421784
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               
tataTGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCCGTATTCATAAACTTGTGTGCAAcggcg                                                                                                                                                                                                                                    >  1:6960/1‑238 (MQ=255)
     gggTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGCCATCATCATCTGGGGGATTGGCAACGGCGGGCCACCCACCGGGATTCATAACCCTTGGCGCAAagggggggttt                                                                                                                                                                                                                              >  2:586928/1‑234 (MQ=255)
        tgtCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGTGCAACCGCCCGGTGTTCCTAACCTTTGGGGCAAtcgcgggctctct                                                                                                                                                                                                                            >  1:304079/1‑231 (MQ=255)
        tgtCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCTTAACCTGTGGGGCAACGGCGGCTTCTTCa                                                                                                                                                                                                                           >  1:726923/1‑239 (MQ=255)
        tgtCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCATAACCTGTGGGGCAACGGCGGCttcttcg                                                                                                                                                                                                                           >  1:92261/1‑238 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGCTTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCTAAACCTGTGGGGAAACGGGGGgttcttacat                                                                                                                                                                                                                         >  1:1044978/1‑235 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGGAACCGGCCGGAATTCATAACCCGGGGGGGCACGGGGGGTTTTTCAga                                                                                                                                                                                                                         >  2:378681/1‑238 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGGAACCGACCGGGATTCAAAACCTTTGGGGGCACGGCGGCTTCTTCCGt                                                                                                                                                                                                                         >  1:667727/1‑239 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGGGCAACGGCGGCTTCTTCAGt                                                                                                                                                                                                                         >  1:354837/1‑239 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAAACTTTGGAGGGACGGCGGCTTCTCCAgg                                                                                                                                                                                                                         >  1:350650/1‑238 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCTTAACCTGTGGAGCAAAGGCGGGCTCTCCAgg                                                                                                                                                                                                                         >  1:323468/1‑238 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCATAACCTGTGGAGCAACGGCGGGCTCTTCCGt                                                                                                                                                                                                                         >  1:30950/1‑239 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGCCAACGGCGGGAAACCGACCGGTATTCAGAACCTTTGGTGAACCGGCGCGTTCTTCCGt                                                                                                                                                                                                                         >  1:706530/1‑239 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGCTTCGGCTTCCTCATCTGGGGGGTTGGCAACGGCGGGGCACCGACCGGGATTCCTAACCCGTGGAGTAAcaggaggtttttcaca                                                                                                                                                                                                                         >  2:458941/1‑224 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCCCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCATAACCTGTGGAGCAAAGGGGGCTTCTTCAga                                                                                                                                                                                                                         >  2:543935/1‑238 (MQ=255)
          tCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCACCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCCACCGCCCGGTATTCATAACCTGGTGGGCAAAGGGGGGTTCTTCCGt                                                                                                                                                                                                                         >  1:89494/1‑239 (MQ=255)
          tCTGGTTCCCGACGGGGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGCAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGCACACGCGTGGCAACCTCCCGGTATTCCAACACTTTGGCGCAACGGCGGGTTCTTCAGt                                                                                                                                                                                                                         >  1:1006224/1‑239 (MQ=255)
            tGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGCCGGGCAACCGCCCGGCATTCATAACCTGTgggggaaacggggcgcttttgcgaa                                                                                                                                                                                                                       >  2:806031/1‑216 (MQ=255)
                   cGACGGTTCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACggctgg                                                                                                                                                                                                                <  1:586928/239‑1 (MQ=255)
                         ggCCGCACTGGATTTGGGTACTGGGCGTGGGGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTgg                                                                                                                                                                                                           <  2:726923/237‑1 (MQ=255)
                           ccGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCTCCCGGGATTTCAAACCCTTGGTGGACCGCGGGCCCCTTCAGGAAAGGCCGGCtttg                                                                                                                                                                                                           >  1:538232/1‑234 (MQ=255)
                           ccGCACGGGATTTGGGTACTGAGCGGGGTGCTGATCAGTTGCGCCGTAAACCTGATGAGCGGGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGGCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCa                                                                                                                                                                                                         <  1:268314/238‑1 (MQ=255)
                                 tGGATTTGGGTGCTGAGCGTGGGGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTaa                                                                                                                                                                                                   <  1:355854/238‑1 (MQ=255)
                                   gATGTGGGTACTGGGCGTGGGGCTGATTATTTGCGCCGTAAACCTTATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTgg                                                                                                                                                                                                           <  1:776560/228‑1 (MQ=255)
                                           tACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCa                                                                                                                                                                                        <  1:541945/238‑1 (MQ=255)
                                             cTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCCTAACCTTTGGGGCAACGGCGGCTTCTTCAGTAACGGCGGGCTTgcccggggatagtgcttgcgaaa                                                                                                                                                                                      >  1:126207/1‑217 (MQ=255)
                                             cTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGGAATGTCGTTGCAAAt                                                                                                                                                                                     >  1:866138/1‑239 (MQ=255)
                                                tgCGTGTGGCTTATCATTTGGGCTGTAAACCTGATGGGCGTGAAGGTAGTCGGGGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGGTTGTCCCCGGTGTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGt                                                                                                                                                                                  <  2:212111/238‑1 (MQ=255)
                                                aGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGGATTCTAAACCTTTGGGGCAACGGCGGCGTCTTACGTAACCGTGGGcctggcctggggagtacggcaccaaaggg                                                                                                                                                                                   >  2:128581/1‑220 (MQ=255)
                                                aGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGCATTCCTAACCTGGGGAGCAACGGGGGCGTCTTCAGGAACGGGCGGCTTTGccagggtggttcggtgcaccagtg                                                                                                                                                                                   >  2:840213/1‑216 (MQ=255)
                                                     gttgcgtatctttttggcCGTAAACCTGATTAGTGGGAATGTTTTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATTATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGt                                                                                                                                                                             <  2:856716/223‑1 (MQ=255)
                                                      gttcTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGt                                                                                                                                                                             <  1:614910/235‑1 (MQ=255)
                                                          ttaTCATTTGCTCGGTAAACCTGATTAGCGTGAAGGTATTCGGTTAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCt                                                                                                                                                                        <  1:993660/237‑1 (MQ=255)
                                                            aTCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGc                                                                                                                                                                                         <  1:346652/220‑1 (MQ=255)
                                                              cATTTGGGCCGGAAACCCGGGGGGCGGGGAGGGATTCGGGTGGTTGGAATTTGGGTTTTCGTTCCTTAAAGTCGCCACCTTAAGCAGCATGATTGTCGCCGTTTTCGGCATAATCAGCTGGGGGATTGGCAACGGGGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACg                                                                                                                                                                    <  2:538232/239‑1 (MQ=255)
                                                                 ttGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGGGCAACGGCGGCTTCTTCAGTAACGGCGGGCTTGGCATGGGAATGGCGTTGCAAATGGGGAGGGTTGCTTTCGGTg                                                                                                                                                                 >  1:791431/1‑239 (MQ=255)
                                                                 ttGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGGAAAGGCCGGCATGGGATGGTAAATTCGGTTGCAATGGGGAGGTTTGCTTAAgggg                                                                                                                                                                 >  1:547400/1‑237 (MQ=255)
                                                                 ttGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCCGTATTCATAACCTGTGGGGCAACGGCGGGTTCTTCCGGAACAGCGGGGTTGGCAGTGTGATGTCGTTGGAAAAgggggggtgtgttttcgtgg                                                                                                                                                                 >  2:1027721/1‑221 (MQ=255)
                                                                                      cGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGGGCAACGGCGGCGTCTTTAGTAAAGGGCGGCTTGGCAGTGGAATGTTGTTGCAAATGGTGATGTTTGTTTACgggggggaggaaaaaaatggggtga                                                                                                                                            >  2:1041507/1‑220 (MQ=255)
                                                                                              tttgcggggtgCTGGATTTTTGGTTCTTTTTTTTTAATGTCGCCACCATCATCATCATGATTGGCGCCGGTTTCGGCATAATCATCTGGGGGATTGGCAAGGGCGGGCAACCGACCGGTATTCATAGCCTGTGGAGCAACTGCGGCTTCTTCAGTAACGGCTGGTTTGGCATGGTAATGTCGTTGGAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGt                                                                                                                                    <  2:1007069/229‑1 (MQ=255)
                                                                                                       gttgggATTTTTGGTCCTCGTTCTTTAAAGTCGCCACAATCATCACCATGATTGTCGCCGGTTGCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTTTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGaa                                                                                                                            <  1:378681/234‑1 (MQ=255)
                                                                                                               ttttgtttCTCTTTTTTTAAAGTCCCCACCGGCATCATCTGGATTGTGCCCGGTTTGGCCATCATCATCTGGGGTATTGGCAAGGCGGGGCAGCCGCCGGGTATTCAAAACCTGGGGAGCACCGGAGGTTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTga                                                                                                                   <  2:193706/233‑1 (MQ=255)
                                                                                                               ttttGGTTTTCTTTTTTTAAAGCCCCCCCCACTCTCATCAGGATGGTCGCCGGGTTCGGCATCACAATCGGGGGGATGGGCACCGGGGGGCAACCGCCCGGTATTCATAACCTGTGGAGCAACGGCGGTTTCTCCAGTAACGGTTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTga                                                                                                                   <  2:30950/236‑1 (MQ=255)
                                                                                                                               ttaaagcgcccacctCCATCATCATGATGGCGGGCGGTTCGGGAAGCATCATCGGGGGGATTGGGAGGGGGGGGCAACCGACCGGTTTTCAAACCTTGTGGAGCAACGGCGGTTTCTACAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGAAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGt                                                                                                   <  2:354837/230‑1 (MQ=255)
                                                                                                                                        ccaccaTCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGcttctt                                                                                                                                                                                                                             <  2:867176/109‑1 (MQ=255)
                                                                                                                                        ccaccaTCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGcttctt                                                                                                                                                                                                                             >  1:867176/1‑109 (MQ=255)
                                                                                                                                                      gtttttGTCGCCGTTTCCGGGTTCCTCATCGGGGGGATTGGAAGGGGCGGGCCACCGACGGGTATTCATACCCTTGGGAGCAAGGGCGGTTTCTTCAGTAACGGGGGGCTTGGCATGGTAATGGCGTGGCAAATGGTGATGTTTGCTTACGGGGGGATGGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCg                                                                            <  2:547400/235‑1 (MQ=255)
                                                                                                                                                          ttGCCGCCGGTTGCGGCATCATCATCTGGGGGATTGGAAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTAtt                                                                        <  1:135127/239‑1 (MQ=255)
                                                                                                                                                              ggtgggtgggcgctctatattgggggggATGGGCAGGGGCGGACACGCGACGGGTATTAATACCCGTGGGAGCACCGGGGGCTTTGTCGGAAAGGTCTGGTTTGGAATGGTAATGTCGTTGCAAATGGGGATGTTTGCTTAGGGGGGGTGGGAAATTATCGGGATTTCCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTg                                                                     <  2:922769/215‑1 (MQ=255)
                                                                                                                                                                    tttgggtctcattctgggggggATTGGAAGCGGCGGGCAACCGACCGGTATTCATAACCTGGGGAGCAACGGCGGCTTCTTCAGTAAGGGGTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTc                                                               <  2:125623/221‑1 (MQ=255)
                                                                                                                                                                     ttgggctttcacttcTGGGGGGTTGCAAAGGGGGGGCAACGACCCGGTTTTCAAACCTTGTGGAGCAAGGGGGATTTTTACAGTAACGGCTGGTTGGGCATGGTAAGGTCGTTGAAAATGGGGATTTTTGCTTAGGGGGGAATCGAAATTATCGGGTTGACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTa                                                             <  2:742626/226‑1 (MQ=255)
                                                                                                                                                                     ttCGGAATCATCCTCGGGGGGGTTGGCACCGGCGGGAACCCGACCGTTTTTATTACCCTGTGGAGCAACGGCGTCTTCTTCAGGAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTCCTGGCGGGGGGATCGAAATTATCGGGATTACCGGCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTa                                                             <  2:1044978/239‑1 (MQ=255)
                                                                                                                                                                          catcttcatCTGGGGGATTGGCAACGGCGGGCAACCGTCCGGTATTCATAACCTGTGGAGCAACGGCGGTTTCTTCAGTAACGGCTGGCTTGGCATGGTAATTTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGt                                                                                                   <  1:420441/196‑1 (MQ=255)
                                                                                                                                                                             tatagtcggggggATTGGCAACGGCGGGCAACCGACCGGTTTGTATAACCTTTGGAGCAACGGCGGTTTCTTCAGTAACGGCGGGCTTGGAATGGTAATGTCGTTGAAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTa                                                     <  1:1027721/231‑1 (MQ=255)
                                                                                                                                                                             actcatcTGGGGGATTGGCAACGGCGGGCAACCGACCGGTTTTCATAACCTGTGGAGCAACGGCGGTTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTa                                                     <  1:840213/237‑1 (MQ=255)
                                                                                                                                                                                    tGGGGGATTGGCAACGGCGGGCAACCGGCCGGTTTTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGTTGGCTTGGCATGGTAATGTCGTTGAAAATGGTGATTTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGtt                                              <  1:851391/239‑1 (MQ=255)
                                                                                                                                                                                             ggcacgggcggGCACCCGTCTGGTTTTCATACCCGGGGGAGCAACGGCGGTTTTTTCATTAAGGGCTGGCTTGGCATGGTAATGTCGTTGCAAAGGGTGAGTGTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAGGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTAt                                     <  2:350650/233‑1 (MQ=255)
                                                                                                                                                                                                         tataagggacagtttctaacccTGGGGGGCAGCGGGGGTTTCTTCAGTAACGGTGGGCTTGGAATGGTAATGTCGTGGAAAATGGTGTTGTTTGCTTCGGGTGGGACCGAATTTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTAccc                         <  2:888100/219‑1 (MQ=255)
                                                                                                                                                                                                          aaCCGACCGGTATTCATAACCTGGGGAGCAACGGGGTCTTCTTCAGTAACGGCTGGCTGGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTAGCGGGATTCCCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTAccc                         <  1:233658/238‑1 (MQ=255)
                                                                                                                                                                                                            ccgaccgGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGGGAAATCGATACCGCGTGCGATTAACTCCGTGCCGGTGCGTATTCTg                                                                     >  1:194268/1‑192 (MQ=255)
                                                                                                                                                                                                                   gTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGCTTAACTCCGGGGCGGTGCGGAATCTGGGGGTCTACGTCGGGACGCTGTTCGTCCTTATGTCTATTTACCCGGGGAATCAg               >  2:316307/1‑239 (MQ=255)
                                                                                                                                                                                                                       tCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGGTg           >  1:927111/1‑239 (MQ=255)
                                                                                                                                                                                                                             ccTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACt      >  1:527116/1‑238 (MQ=255)
                                                                                                                                                                                                                              cTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGGATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTg     >  1:969974/1‑238 (MQ=255)
                                                                                                                                                                                                                                gtgGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGGATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGCTgctccttat   >  1:888100/1‑230 (MQ=255)
                                                                                                                                                                                                                                gtgGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGGGCCGATGCGTATTCTGGTGTTCTACGTCGGGACGCCGTTCGTCATTATGTCTTTCTACCCGTGGAATCAGGTTGGCATTGCCg  >  1:618876/1‑239 (MQ=255)
                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               
TATATGGGTGTCTGGTTCCCGACGGTGCCGCACTGGATTTGGGTACTGAGCGTGGTGCTGATCATTTGCGCCGTAAACCTGATGAGCGTGAAGGTATTCGGTGAGCTGGAATTCTGGTTCTCGTTCTTTAAAGTCGCCACCATCATCATCATGATTGTCGCCGGTTTCGGCATCATCATCTGGGGGATTGGCAACGGCGGGCAACCGACCGGTATTCATAACCTGTGGAGCAACGGCGGCTTCTTCAGTAACGGCTGGCTTGGCATGGTAATGTCGTTGCAAATGGTGATGTTTGCTTACGGTGGGATCGAAATTATCGGGATTACCGCCGGTGAAGCGAAAGATCCTGAGAAATCGATACCGCGTGCGATTAACTCCGTGCCGATGCGTATTCTGGTGTTCTACGTCGGTACGCTGTTCGTCATTATGTCTATCTACCCGTGGAATCAGGTTGGCACTGCCG  >  NC_000913/421322‑421784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: