Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,065,603 T→A 26.8% I55F (ATC→TTC)  yihO ← putative sulphoquinovose importer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,065,6030TA26.8% 156.9 / 22.4 101I55F (ATC→TTC) yihOputative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (49/22);  new base A (10/18);  total (59/42)
Fisher's exact test for biased strand distribution p-value = 3.18e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

CAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCT  >  NC_000913/4065370‑4065832
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     
caGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGA‑GCCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGGCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGGTAATc                                                                                                                                                                                                                                   >  1:68681/1‑238 (MQ=255)
   aCTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGAGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATACAGGGCCGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCAGGTAG‑AGTAAAAAT‑CCGGTGAG‑CCTATC‑GGTAA‑ACGCGGTAAAAAAAT‑TCGCCAAC‑AA‑AAAAAAAAACCCGc                                                                                                                                                                                                                               >  2:876112/1‑239 (MQ=255)
     tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGGGTCG‑AGTAAAAAT‑CCGGTGGG‑CATATC‑GGTAA‑ACGCGTAAAAAAACT‑TCGCCACC‑AA‑AAAGAAAATCCGGCCa                                                                                                                                                                                                                             >  2:316137/1‑239 (MQ=255)
     tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCCCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GTTAA‑ACGCGGTAAAAAACT‑TCGCCCCC‑AG‑AACGCAAATccgccca                                                                                                                                                                                                                             >  1:512234/1‑234 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAGGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATa                                                                                                                                                                                                                           >  1:131818/1‑239 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCTCGATGCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AT‑AAAGATAATCCCGCCATa                                                                                                                                                                                                                           >  1:567319/1‑239 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATa                                                                                                                                                                                                                           >  1:733376/1‑239 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAAAT‑TCGCCACC‑AG‑AAAGAAAAACCCGCCaaa                                                                                                                                                                                                                           >  2:1083069/1‑237 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CAGATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑GG‑AAAGTAAATCCCGCCATa                                                                                                                                                                                                                           >  1:499058/1‑239 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGGGGG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGAAAATcccccaaaa                                                                                                                                                                                                                           >  2:608746/1‑233 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAA‑CCGGGAAG‑CATAAC‑GGTAA‑ACCCGGTAAAAAAAT‑TCGCCAAC‑AA‑AAAAAAAAACCCGCCaaa                                                                                                                                                                                                                           >  2:486743/1‑237 (MQ=255)
       tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCAAGTCG‑AGTAAAAAT‑CCGGTAAG‑CATATC‑GGTAA‑ACTCGGTAAAAAACT‑TACCCACC‑CG‑AAAGAAAATCCCGCCATa                                                                                                                                                                                                                           >  1:1016194/1‑239 (MQ=255)
             aGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAAATGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGGAAAAAACT‑TCGCCACA‑GA‑AAAGATAATCCCGCCATAgaacga                                                                                                                                                                                                                     >  1:119575/1‑234 (MQ=255)
               tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑CG‑AAAGAAAATCCCGCCATAGTAAGCAg                                                                                                                                                                                                                   >  1:225100/1‑239 (MQ=255)
               tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCa                                                                                                                                                                                                                    >  1:485383/1‑238 (MQ=255)
                 cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATATTAAGCAGGc                                                                                                                                                                                                                 >  1:1013372/1‑239 (MQ=255)
                 cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACACGGTAAAAAACT‑TCGCCACC‑AA‑AAAGAAAAACCCGCCAAAGaaaaaagga                                                                                                                                                                                                                 >  2:640419/1‑233 (MQ=255)
                 cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATACC‑GGTAA‑ACGCGGTACAAAACT‑TCACCACC‑AG‑AAAGATAAGCCCGCCATAGTAAACAGGc                                                                                                                                                                                                                 >  1:232166/1‑239 (MQ=255)
                 cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCAAATATTTT‑TCCACCAGTAG‑ACACAAAAT‑CCGGTAAG‑AATAAC‑GGAAA‑AACCGGTAAAAACCT‑TTGCCCCA‑CA‑AAAGATAAACCCGCCaaaaaaaaaagaa                                                                                                                                                                                                                 >  2:568381/1‑227 (MQ=255)
                 cAAACATCAAAAACCGCGGGGTGCCAGTCGTCTTTTTACCCGAAAAAAAAAG‑GGGGGT‑GGCAATAAACGGGA‑GTGT‑TGCAATTACCGCCCCCGAAC‑CCGCTG‑CATAAAAAAAAAGGGGAAAAAATTTGC‑TTTTAGGCCAAATTTTTT‑CCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑CCGCGGAAAAAAACT‑TCGCCCCC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                                 <  2:1009450/239‑1 (MQ=255)
                        catAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAAATAATCCCGCCATAGTAAGCAGGCATCCCTa                                                                                                                                                                                                          >  1:811516/1‑239 (MQ=255)
                         atAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAAAT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAACAAGGCATCCCTaa                                                                                                                                                                                                         >  1:144992/1‑239 (MQ=255)
                               ggccctgttggaatttgttttttAACCGGAAAAACAAA‑GGGGGG‑GGCTAAAAACAGGG‑GGCT‑TTCAAATAACCCCGCCCGAACCCGTGG‑AATAAAAAAAAAGGGGACAAAATGTCC‑TTTTAGCCCAAATATTTT‑CCCGCGAGTTG‑AATAAAAAT‑CCGGTGAA‑CATATC‑GGAAA‑ACGCGTAAAAAAATT‑TCGCCACC‑AA‑AAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCAt                                                                                                                                                                                                    <  2:901148/220‑1 (MQ=255)
                                gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                                  >  1:278920/1‑239 (MQ=255)
                                gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                                  >  1:127562/1‑239 (MQ=255)
                                gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCACCCCTAATTCaaca                                                                                                                                                                                                  >  2:184025/1‑236 (MQ=255)
                                gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGGGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAAACCCGCCAAAGAAAACAGACAACCCTAAtaacacg                                                                                                                                                                                                  >  2:303947/1‑233 (MQ=255)
                                      tgcaatttgtgtttttAAGCGTAAAAAAAAG‑GGGGGG‑GGGAAAAAAGTGGA‑GTGT‑TAAAATTACCGCGGCCGGAC‑CCACTG‑CTTAAAAAAGAAGGGGAAGAAATTTGC‑TTTTAGGCCCATTTTTGT‑GCCGCGAGTCA‑AATAAAAAT‑CTGGTGAC‑CATAGC‑GGTAA‑ACGAGGTAAAAATCT‑TCGCCACC‑AG‑AAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata                                                                                                                                                                                             <  2:232166/226‑1 (MQ=255)
                                           aTCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAttt                                                                                                                                                                                       >  1:130719/1‑239 (MQ=255)
                                             tcgttttttAAACCGCAAAAAAAAGGGTGGG‑GGTAATAAAGGGGG‑GCGT‑TCAAATTAGCCCCGCGGGAC‑CCTCTT‑AAAAAAAAAAAAAGGGAAGAAATTTGC‑CTTGAGGCCAAATTTTTT‑CCCGCGAGTGA‑AGAAAAAAT‑CCGGGGAG‑CATATC‑GGAAA‑ACGCGGAAAAAAACT‑CCGCCACC‑AA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                                      <  2:701723/234‑1 (MQ=255)
                                              tttttttaaccccgaaaaaaaaG‑GGGGGT‑GGATAAAATCTGGG‑GCTT‑TAAAATAACCGCCCCCGGAA‑CCGTTG‑AATAAAAAAAAAAGGGAAGATATTTCT‑CTTTCGCCCAATTTTTTT‑TCCGCGAGTCG‑AATAAAAAC‑CCGGTGAC‑CATATC‑GGTAA‑CCGGGGAAAAAATCC‑TCGCCCCA‑AG‑AAATAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                                    <  2:560912/224‑1 (MQ=255)
                                               tttttttAACCCGAAAAAAAAA‑GGGGGTGGGAAAAAAACGGGG‑GCTT‑TGAAATTACCCCGGCGGGAA‑CGGTTG‑AATAAAAAAAAAAGGAACAAAATTTGC‑TTTTAGGCCCAATATTTT‑TCCGGGAGTAG‑AGAAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGAAAAAACCT‑TCGCCACA‑AG‑AAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                                    <  2:270596/236‑1 (MQ=255)
                                                cgtTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGAATCCCTAATTCATCGGTATAAAATTTgagga                                                                                                                                                                                  >  1:441236/1‑239 (MQ=255)
                                                  ttttTACCCGCAAAAAAAAGGGGGGT‑GGCAAAAAATTGG‑‑GCTTGTGAAATCACCCCCGCCGCAC‑CCGCAA‑TAAAAAAAAAAAAGGGAAAAAATTTGC‑CTTTGGGCCAATTTTTTT‑CCCGCAAGTGG‑AGAAAAAAC‑CCGTTGAG‑CATAGC‑GGAAA‑ACGCGGTAAAAAACT‑CCGCCCCA‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgaggag                                                                                                                                                                                 <  2:733376/239‑1 (MQ=255)
                                                     tAACCAGAAAAAAAAA‑GGGGGG‑GGGAAAAAAAGAGA‑GGGT‑TGAAAATGACCGCGCCGGAA‑CCCGTGTTATAAAAAAAAAGGGGACAAAATTCGG‑TTTTGGCACCAGTATTTT‑TCGGAGATCCG‑AAAAAAACT‑CCGG‑GAGTCATATG‑GGAAA‑ACGGGGAAAAAACCT‑CCCCCACA‑AA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATa                                                                                                                                                                              <  2:1016194/239‑1 (MQ=255)
                                                       cccgaaaaacaaaG‑GGTGTG‑GGAAATAAA‑TGGAGGCGT‑TGCATTCACCGCCGCGGAAC‑CCGCTC‑AAAAAAAAAAAAAGGAACAAAATTTGT‑CTTTGGGCCCAATTTTTT‑GCAGGGAGACG‑AGTAAAAAT‑CGGGTAAG‑CATATC‑GGTAA‑ACGCGTAAAAAAACT‑TCGCCACA‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                                           <  2:398146/233‑1 (MQ=255)
                                                                 aaaa‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCACGCCATAGTAAGCAGGGATCCCTAATTCACCGGGATAAAATTTGAGGAGATACAGAGTACCGagg                                                                                                                                                                 >  1:504776/1‑237 (MQ=255)
                                                                          gt‑ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCAt                                                                                                                                                         >  1:772754/1‑239 (MQ=255)
                                                                          gt‑ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAGGTACCGATGCAAAGcct                                                                                                                                                         >  1:389985/1‑237 (MQ=255)
                                                                             ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGGAGTTACAGAGTACCGATGCAGAGCATTa                                                                                                                                                       >  1:338563/1‑239 (MQ=255)
                                                                             ggCGATAAACTGGA‑GCGT‑TGCAATTAACGCAGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGCGGAGATACAGAGAACCGCTGCAGAGCATTa                                                                                                                                                       >  1:187535/1‑239 (MQ=255)
                                                                                        ggA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGTAACAGGGAACCGAGGCAGAGCATTAAATTCGAACCg                                                                                                                                            >  1:337540/1‑239 (MQ=255)
                                                                                        ggA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCAGCCATAGTAAGCAGGCACCCCTAATTCATCGGTATAAAATTTGGGGGGAAAAAAATAACCGATCCAGAGCATTAAATTCAAACCg                                                                                                                                            >  2:648874/1‑239 (MQ=255)
                                                                                             gtt‑tGCACTTACCGCCGTCGCAC‑CCTCT‑‑TATAAAAAAAAAAGGGGAAAAATTTGCGCTTGAGG‑CCAATAATTT‑TCCCCGAAGCGAAAAAAAAAG‑CCGGCGAC‑TATATA‑GATAA‑CCGCGATAAAACTCT‑TCGCAACC‑AG‑AAAGAGAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACg                                                                                                                                         <  2:68681/236‑1 (MQ=255)
                                                                                               t‑tGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTc                                                                                                                                       >  1:436422/1‑238 (MQ=255)
                                                                                                  gCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGAAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAAGCATTAAATTCAAACCGACGTccc                                                                                                                                     >  2:114075/1‑236 (MQ=255)
                                                                                                     aTTAACGCCGCCGGAA‑CCGCTG‑CATAAAAAAAAAAGGGACGAAATTTGC‑TTTTAGGCCCAATTTTTT‑CCCGCGAGGCG‑AATAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑CCGCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCccat                                                                                                                                 <  1:387569/239‑1 (MQ=255)
                                                                                                      ttAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGGCGCccat                                                                                                                                 >  1:1001162/1‑238 (MQ=255)
                                                                                                             cgccgGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTTAGGAGATACAGAGTACCGATGCAGAGCATTa                                                                                                                                                       >  1:748694/1‑209 (MQ=255)
                                                                                                              gccgGAA‑CCGCTG‑CATATAAAATAAAGGGACGAATTTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGAGGAGATAAAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGc                                                                                                                        >  1:943050/1‑239 (MQ=255)
                                                                                                                          tG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTTTAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCccatacctggctcttatacacat                                                                                                              >  1:60853/1‑222 (MQ=255)
                                                                                                                            caataaAAAAAAAAGGGAAGAAATTTGC‑TTTTAGGCCAATTATTTT‑CCCGCGAGTCG‑AGTAAAAAC‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGAAAAAAACT‑TCGCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCg                                                                                                            <  1:1045845/237‑1 (MQ=255)
                                                                                                                                  aaaaaaaaGGGAAAAAATTTGC‑CTTAAGGCCAAATTTTTT‑CCCGGGAGCAG‑AGAAAAAAT‑CCGGTGAG‑CATAGC‑GGTAA‑ACGCGGAAAAAAACT‑TCGCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                      <  1:114075/239‑1 (MQ=255)
                                                                                                                                   aaaaaaaaGGACCAAATTGTC‑CTTTGAGTCCAATTTTTTGCCCGAGAGAAG‑ATAAAAAAT‑CCGGTAAG‑CA‑AGC‑GGAAACACGCGGAAAAAAACT‑TCGCC‑CC‑AGAAAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                      <  2:504776/239‑1 (MQ=255)
                                                                                                                                     ataaaGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAg                                                                                                   >  1:638569/1‑239 (MQ=255)
                                                                                                                                             aaaaaaTTTGC‑CTTGAGCCCAAATATTCT‑GCCGCGAGTAG‑AAAAAAACT‑CCGGTGAG‑CATTCG‑GGTAA‑CCGCGTTAAAAAACT‑TCGCCCCA‑AA‑AAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAt                                                                                            <  1:640419/235‑1 (MQ=255)
                                                                                                                                              cGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAg                                                                                                                                                                                                                      >  1:901088/1‑115 (MQ=255)
                                                                                                                                              cGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCAAACCAAAGGCGATTTTTTCCCCCAGATTCAGTTTTAAtta                                                                                          >  2:680391/1‑237 (MQ=255)
                                                                                                                                                     tGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGCTTTTTTCCCGCAAATTCAGTTTTAATTTCAGTGGa                                                                                   >  2:567156/1‑239 (MQ=255)
                                                                                                                                                            tAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGCCGCCCATACCATAGGCGATTTTTTCCCCAAAATTCAGTTTTAATGTAAGTGGATTATgg                                                                             >  2:514077/1‑239 (MQ=255)
                                                                                                                                                             agcccaaaTTTTTT‑CCCGCGACTCG‑AGAAAAAAG‑CCGGTAAG‑TAT‑TG‑GGGAA‑AATGGGAAAAAAACTCCCCCCACA‑AA‑AAAAATAACTCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  2:144992/233‑1 (MQ=255)
                                                                                                                                                             agccacAATATTTT‑TCCGCGAGTCA‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑CCGCGGTAAAAAACT‑TCGCCACA‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  1:569923/234‑1 (MQ=255)
                                                                                                                                                                   aaTATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTTTGTTCAGACa                                                                      >  1:77030/1‑239 (MQ=255)
                                                                                                                                                                   aaTATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACa                                                                      >  1:558506/1‑239 (MQ=255)
                                                                                                                                                                    atatttttttccgaggtgtaagaaaaaaT‑GCGGTGAA‑CATACC‑GGAAA‑AGGGGGAAAAAACCT‑CCCCCCAA‑AA‑AAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGAc                                                                       <  2:131818/216‑1 (MQ=255)
                                                                                                                                                                       tttt‑cCCGGGAGACG‑AGAAAAAAC‑CCGGTGAG‑CATCTA‑GGAAA‑CCGCGG‑AAAAAACTCTCCCCACC‑AG‑AAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAAt                                                                  <  1:996985/239‑1 (MQ=255)
                                                                                                                                                                          ttcccgaaattcg‑agaAAAAAC‑CCGGTGAG‑CATCTC‑GGTAA‑CCGCGATAAAACACT‑CCGCCCCC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  1:486743/229‑1 (MQ=255)
                                                                                                                                                                          ttcccGCGAGTCT‑AAAAAAAAC‑CCGATGAA‑CATATG‑GGAAA‑CAGGGGAAAAAACCT‑TCCACACC‑AA‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  1:567156/236‑1 (MQ=255)
                                                                                                                                                                          gtcgcgcgAGTAG‑AGTAAAAAT‑CCGGGTAG‑AATATC‑GGTAA‑ACGAGATAAAAACCT‑TCGCCACC‑AG‑AAAGAAAATGCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACTTAATCa                                                                <  1:568381/235‑1 (MQ=255)
                                                                                                                                                                           ttccggggggagagatAAAAAC‑CCGGTGA‑‑AAAATCGGGAAA‑ACGGGTAAAAAAACT‑TCGCCACA‑AA‑AAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  2:130719/224‑1 (MQ=255)
                                                                                                                                                                           ttcccggggttcaaatAAAAAC‑CGGTTAAG‑CATATA‑GGAAA‑CCGCGGAAAAAAACT‑CCCCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  1:608746/224‑1 (MQ=255)
                                                                                                                                                                            ttcccggaatgcagtAAAAAA‑CCCGGTAA‑CAAATC‑CGGAA‑ACCGGGTAAAAAACC‑TCCCCACCAAA‑AAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  1:514077/227‑1 (MQ=255)
                                                                                                                                                                            tCCGGGAGT‑G‑AATAAAAATCCCGGTGAG‑AAATTG‑GGAAA‑ACGCGGTAAAAACCT‑GCGCCCCA‑AA‑AAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                                              <  1:1083069/239‑1 (MQ=255)
                                                                                                                                                                              cgcgAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCAGATTCAGTTTTAATGTCATTGGATTATGGTCAGACaaaacaacc                                                              >  2:285819/1‑232 (MQ=255)
                                                                                                                                                                                cgagtcg‑agtAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGCGCATTAAATTCGAACCAACGTCGCCCATATCATAGCCGATTTTTTCCCGCAGATTCAGTTTTAATACCATTGGATTATGGTCAGACTTAATCACTCCtt                                                          >  1:1055582/1‑239 (MQ=255)
                                                                                                                                                                                cgagtcg‑agtAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGGGATTTTTTCCCGCAGATTCAGTTTTAATGTACATGGATTATGGTTAGAACAAAACACTCCtt                                                          >  2:729659/1‑239 (MQ=255)
                                                                                                                                                                                cgagtcg‑agtAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGGCAGAACTAATAACTCCtt                                                          >  1:668856/1‑239 (MQ=255)
                                                                                                                                                                                               aaaacccggtgacaattgggtaaa‑aCGCGAAAAAAACCC‑TCCCCACC‑AG‑AAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCg                                               <  2:943050/217‑1 (MQ=255)
                                                                                                                                                                                                  cgggggAG‑CTTATG‑GGAAA‑ACGCGGAAAAAACCT‑CCGCCCCA‑AA‑AAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAggg                                          <  2:127562/234‑1 (MQ=255)
                                                                                                                                                                                                  accgcgtgtgcataatgcgaa‑aCGGGGAAAAAAACC‑TTCCCCCC‑AGAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCa                                             <  2:772754/220‑1 (MQ=255)
                                                                                                                                                                                                   gggTGAG‑AATAGG‑GGAAA‑ACG‑GGGAAAAAAAT‑TCCCCACCAAA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGc                                         <  1:285819/238‑1 (MQ=255)
                                                                                                                                                                                                     ggagg‑CATAGC‑GTAAA‑ACGGGGAAAAAAACT‑TCGCCCAA‑AG‑AAAGAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                                        <  1:729659/234‑1 (MQ=255)
                                                                                                                                                                                                         g‑CATATC‑GGAAA‑ACGTGAAAAAAACTT‑TCCCCACC‑AG‑AAATATATCCCCGCCATAGTAAGCAGGCATCCCTAATTCAGCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcgtcgt                                    <  1:648874/238‑1 (MQ=255)
                                                                                                                                                                                                             ttgc‑GGTAA‑ACGCGAAAAAAACCT‑ACACAACA‑AA‑AGATACACTCCCGCCATAGTAAGCAGGCATCCCTAATTCAACGGTATAAAATTGCAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCTAACCGACGTCGCCCATACCATAGGCGATTTTTGCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  1:54610/236‑1 (MQ=255)
                                                                                                                                                                                                              tttgggaca‑aCGCGGAAAAAAATC‑TCCCCAAA‑AA‑AAAAATAACCCCGGCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  2:1023656/231‑1 (MQ=255)
                                                                                                                                                                                                              tttatgaaa‑aCGGGGAAAAAACCC‑TCCCCAAC‑AG‑AAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  2:697068/232‑1 (MQ=255)
                                                                                                                                                                                                                 ccgaaaaccgggaaaaaaatCC‑TCCCCACA‑AA‑AAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttatta                              <  2:558506/225‑1 (MQ=255)
                                                                                                                                                                                                                      a‑aCGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTTTAACGCCCCCCAGGGCGCCGTttattattttt                         >  1:9150/1‑238 (MQ=255)
                                                                                                                                                                                                                      a‑aCGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttattattatt                         >  1:756622/1‑238 (MQ=255)
                                                                                                                                                                                                                           ggatAAAAAACT‑TCCCCACA‑AG‑AAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACg                      <  1:595647/234‑1 (MQ=255)
                                                                                                                                                                                                                             gTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGCCGTttattattattaacccgg                  >  2:234842/1‑233 (MQ=255)
                                                                                                                                                                                                                                  aactt‑tCGCCACA‑AA‑AAAGAAAACCACGCCAAAGGAAACAGGTATCCCTAATTCATCGGTAAAAAAATTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCggg             <  2:44886/235‑3 (MQ=255)
                                                                                                                                                                                                                                         tccccccaaaaaaaGATAATCCCGGCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACg         <  1:680391/228‑1 (MQ=255)
                                                                                                                                                                                                                                         cGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAAt                                                                  >  1:991918/1‑180 (MQ=255)
                                                                                                                                                                                                                                         cGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTTTAACGCCCCGCAGGGCGTCGTTTATTATTATTAACCCGTGCGTTTACgg        >  1:455110/1‑238 (MQ=255)
                                                                                                                                                                                                                                         cGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACgg        >  1:184476/1‑238 (MQ=255)
                                                                                                                                                                                                                                         cGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCCCAGGGcg                                        >  2:266482/1‑206 (MQ=255)
                                                                                                                                                                                                                                         cGCCACC‑AA‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                                        <  1:266482/206‑1 (MQ=255)
                                                                                                                                                                                                                                                caaaaaaaATAATCCCGCCATAGTAAGCAGGCATCCCTAAATCATCGGTATAAAATTTGAGGAGATACAGAGTTCCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCt  <  2:512234/235‑1 (MQ=255)
                                                                                                                                                                                                                                                caaaaaaGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCt  <  1:316137/235‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                     
CAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAA‑GGTGGT‑GGCGATAAACTGGA‑GCGT‑TGCAATTAACGCCGCCGGAA‑CCGCTG‑CATATAAAATAAAGGGACGAAATTTGC‑CTTTAGGCCCAATATTTT‑TCCGCGAGTCG‑AGTAAAAAT‑CCGGTGAG‑CATATC‑GGTAA‑ACGCGGTAAAAAACT‑TCGCCACC‑AG‑AAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCT  >  NC_000913/4065370‑4065832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑