Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 429,854 T→G 15.0% V117G (GTG→GGG)  secF → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,8540TG15.0% 302.4 / 6.9 114V117G (GTG→GGG) secFSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base T (18/74);  new base G (19/3);  total (37/77)
Fisher's exact test for biased strand distribution p-value = 7.37e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAT  >  NC_000913/429618‑430088
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
gtttttttgggggtgtggggtttttAACTGGGGGCGGGAGTTCCCCGGGTGTAAGTTTATTAAATTTCCGCTCGAAAACCCGGCTGAAATTGCCGTAATGTGTGATTCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGa                                                                                                                                                                                                                                          <  2:508538/217‑1 (MQ=255)
   tattatGGGCGGTCGCGGCTTTAACGGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTa                                                                                                                                                                                                                                       <  1:829016/239‑1 (MQ=255)
      tatGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGCGGAAACAGGCGGACAGGTGTTGGGGCGCCAGGTTCTGAAGGGGGTTacag                                                                                                                                                                                                                                    >  1:408160/1‑236 (MQ=255)
          ggCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAACACGGCGGGCACGGGTGGGGGACCACGGTCTTCAAGGGGATTAAAGaaac                                                                                                                                                                                                                                >  2:371150/1‑237 (MQ=255)
          ggCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCCGGGGGTGGGCGACCCGGGTCTGGAAGGGGATAAACaaa                                                                                                                                                                                                                                  >  2:280224/1‑238 (MQ=255)
           gCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGGTGGGCGGCCAGGTTCTGAAGGGGGTTAACGaaaac                                                                                                                                                                                                                               >  2:1087967/1‑236 (MQ=255)
            cGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGGGAAACCGGCGGCCAGGGGGTTGGGCGCCAGGGTTTTAAGGGGATTAACAAAccc                                                                                                                                                                                                                               >  2:1069892/1‑238 (MQ=255)
             gTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAATTTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTGTAGGCAGCCGGGTTCTGAAGGGGCTTAACCAATccc                                                                                                                                                                                                                              >  1:292729/1‑237 (MQ=255)
             gTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATcca                                                                                                                                                                                                                              <  1:300407/238‑1 (MQ=255)
               gcgaGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGCAATCATGGTCCGTATGCCGCCTGTTGAAGGCGAAACCGGCGGCCAGGGGTTGGGGAGCCCGGTTGTAAAGGGTATTACAGAaccaacca                                                                                                                                                                                                                           >  2:150054/1‑239 (MQ=255)
                 ccgGCTTTAACGGGGGGCTGGATTTCACCGGTGGTACGTTTATTGAAATTACGGCTGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAt                                                                                                                                                                                                                         <  2:343781/238‑1 (MQ=255)
                      tttAACTGGGGGCTGGATTGCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGaa                                                                                                                                                                                                                    <  1:899233/239‑1 (MQ=255)
                        tAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATg                                                                                                                                                                                                                  <  1:212173/239‑1 (MQ=255)
                           cTGGGGGTTGGATTTCCCCGGGGGTACGGTTATTTAAAGTTCCCTCGAAAACCCGGCTGAAATTGAAGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGGCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcag                                                                                                                                                                                                               <  2:782466/239‑1 (MQ=255)
                           cTGGGGGCTGGATTTCACCGGTGGTACGGTTAATGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACAGCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATgcag                                                                                                                                                                                                               <  1:536200/239‑1 (MQ=255)
                                ggttGGTTTTCCCCGGGGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTGGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                           <  2:286486/235‑1 (MQ=255)
                                ggCTGGATTTCCCCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                           <  1:102958/238‑1 (MQ=255)
                                ggCTGGATTTCACCGGTGGGACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGt                                                                                                                                                                                                           <  1:603471/238‑1 (MQ=255)
                                  cTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTTAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGa                                                                                                                                                                                                         <  1:168980/238‑1 (MQ=255)
                                       tttCAACGGTGTGACGGTTAGTGAAATTACTCGCGAAAAACCGGCGGAAATTGACGTAATGCGTGATGCATGGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTa                                                                                                                                                                                                   <  1:983154/239‑1 (MQ=255)
                                          cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCCGGTGTTGGGCAGCCCGGGTCTGAAGGTGATTAACGGATCCACCAATCAGAATGAAGCAGGGAAGCGTATTg                                                                                                                                                                                                >  1:1057614/1‑239 (MQ=255)
                                          cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGATCTGACGGGGATTa                                                                                                                                                                                                                                       >  1:1072141/1‑200 (MQ=255)
                                          cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGAGTTGGGCAGCCAGGGTCTGAAGGGGCTTATAGCACCACCCAATCAGAAGACAGCAGCGAAgacgattg                                                                                                                                                                                                >  1:509255/1‑232 (MQ=255)
                                          cACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGGGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGTCGGTCCGGTGTTGGGGAGCCAGGTTCTGAAGGGGCGTAACGAATCCACCAATCCGAATGCCGTAGGGaacggatttg                                                                                                                                                                                                >  1:1029138/1‑231 (MQ=255)
                                                 tgtACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  1:371150/238‑1 (MQ=255)
                                                 ggtTCGGTTTTTGAAATTTCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  2:880481/239‑1 (MQ=255)
                                                 ggtTCGGTTTTTGAAATTCCCCACGAAAAACCGTGTAAAATTGACGGAAAGCGTGTCGCATTGCAAAAAGCCTGTTTGGAAGAGCCTAGGCTGGAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTCAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  2:176857/239‑1 (MQ=255)
                                                  gtACGGTTAGTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGGATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTtcgt                                                                                                                                                                                         <  1:354068/238‑1 (MQ=255)
                                                        ttatgaaaaTTCCGCCCGAAAACCCGGTGGAAATGGACGTAATGGTGGATTCATTGCAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCg                                                                                                                                                                                  <  2:1077596/233‑1 (MQ=255)
                                                          aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTTAAGGGGGTTAACGAATCCCCCAATCAGAATGCCGCAGGGGAGCGGATTGGGTTCGGCGGGcccga                                                                                                                                                                                >  2:18078/1‑236 (MQ=255)
                                                          aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCAGGTTCTGAAGGGGATTAACGGATCCCCCAAACAGAAAGCAGAAGGGAAACGTGTTGAGtttcggggcccgag                                                                                                                                                                                >  2:484586/1‑227 (MQ=255)
                                                          aTTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTAGGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTTGGCAGCCAGGTTCTGAAGGGGATTAACGAATCCACCAATCAGAAAGCAGCAGTGAAGCGAATTGAAGTCCTCGGGccca                                                                                                                                                                                 >  1:513458/1‑236 (MQ=255)
                                                            ttaatttccgcgcgAAAACCCCGCTGAAATTGACGAAATGCGTGATCATTTGCAAAAGGCCGGTTTGGAGGGGCCGATCCTGCAAAACTTTGGTAGCAGCGATGACAGCAAGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTCCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  2:408160/231‑1 (MQ=255)
                                                            ttaaaTTTCCCTGGAAAAACCGGTTTAAATTGACGTAATGGGGGAGGCATTTAAAAAAGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  2:749494/237‑1 (MQ=255)
                                                            ttaaaTTCCGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  1:1000940/237‑1 (MQ=255)
                                                                  tACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCCGAATGCAGCAGTGAAGCGTATTGGGTTCGTCGGTCCGCGCgggggg                                                                                                                                                                         >  1:598081/1‑238 (MQ=255)
                                                                        cGAAAACCCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGAcc                                                                                                                                                                  <  1:594187/239‑1 (MQ=255)
                                                                               ccGGCTGAAATTGACGTAATGCGTGATGAATTGCAAAAATCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCa                                                                                                                                                           <  1:252054/239‑1 (MQ=255)
                                                                               ccGGCTGAAATTGACGTAAGGCGTGATGCATTGCAAGAAGCAGGTTTGTAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCa                                                                                                                                                           <  1:499070/239‑1 (MQ=255)
                                                                                          tgtggtaattgttgttgtcttGGAAAAAGCTGGTTTGGAAGAGCCGATGCTGAAAAACTTGGGTAGCAGCCATGGCATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGa                                                                                                                                                <  2:317995/220‑1 (MQ=255)
                                                                                           gagttaagggggtaTGTATTGCAAAAAGCCGGTTGTGAAGAGCCGATTCTTCAAAACTTTTGGAGCAGCCATGACATCATGGTCCGTAGGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGAt                                                                                                                                               <  2:287121/226‑1 (MQ=255)
                                                                                             cGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGCGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATg                                                                                                                                              <  1:282941/238‑1 (MQ=255)
                                                                                                atggggttaTGCATTGCAAAAAGCCGGTTTTTAAGGGCCGGTGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCg                                                                                                                                           <  2:504182/230‑1 (MQ=255)
                                                                                                   gCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGATTAACAAAACCCCCAATCAGAATGCAGCAGGGAAGGGGTTTTGGTTCGTGGGTCCGGGCCGGGGGGCCGCCCTTGGGCAAACCGGGGGGGTGGGGTTgg                                                                                                                                       >  2:1042559/1‑238 (MQ=255)
                                                                                                      tGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATg                                                                                                                                     >  1:253310/1‑238 (MQ=255)
                                                                                                       gtggtatggCAAAAAGCGGGTTTGGAGGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGGCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGGTGGGCAGCTAGGTTGTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATgg                                                                                                                                    <  2:619917/230‑1 (MQ=255)
                                                                                                        tttctttGCAAAAAGCCGGTTTTGAAGGGCCGAGGCTTGAAAATTTGGGGAGCAGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCa                                                                                                                                  <  2:902026/234‑1 (MQ=255)
                                                                                                         ttcttttaaaaaaGCCGGTTTTGAAGGGCCGATCCGGCAAAACTTTGGTAGCAGCCATGATATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGCTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                                                 <  2:295436/231‑1 (MQ=255)
                                                                                                         tGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAATCTTTGGTAGCAGCCATGACATCAGGGGCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                                                 <  1:1042559/239‑1 (MQ=255)
                                                                                                         tGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGGCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGATgtgggcg                                                                                                                                  >  1:1001531/1‑232 (MQ=255)
                                                                                                         agCATTACAAAAGGCCGTTTTTGAAGAGCCGATGCTGCAAAACTTTGGTCGCCGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAg                                                                                                                                 <  1:969914/238‑1 (MQ=255)
                                                                                                             tttaaaaaaGCCGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGGAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgct                                                                                                                             <  2:677775/235‑1 (MQ=255)
                                                                                                             ttGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGGTCTGAAGGTGATTAACCAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGGCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCggggtgggagggggg                                                                                                                             >  2:922948/1‑225 (MQ=255)
                                                                                                              ttgaaaaaGCCCGTTTTGAAGGGCCGATTGTGCAAAACTTTGGGAGCGGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  2:1001531/236‑1 (MQ=255)
                                                                                                              tgaaaaaaGCTGGTTTTGAAGAGCCGATTCTGAAAAACTTTGGTAGCAGCCATGACATCAGGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCgctg                                                                                                                            <  2:1008275/236‑1 (MQ=255)
                                                                                                              tgaaaaaaGCCGGTGTGGGACAGCCGTTGCTGCAAAACTTTGGTAGCGGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCTGCgctg                                                                                                                            <  2:975733/236‑1 (MQ=255)
                                                                                                                  aaaaGCCGTGTTTGAAGAGCCGATTGTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTTCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGt                                                                                                                        <  1:511158/239‑1 (MQ=255)
                                                                                                                   aaaGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGGTCTGAAGGGGATTAACGAATCCACCAATCAGGATTCAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGAGGGAGGGGGCAGACGTTGCGCAAACCGGGGCGCTGGGGTtggtggggggggtgggggg                                                                                                                       >  2:929139/1‑226 (MQ=255)
                                                                                                                       ccGTTTTGGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                                                   <  1:362847/239‑1 (MQ=255)
                                                                                                                        cGGTTTTGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                                                   <  1:18078/238‑1 (MQ=255)
                                                                                                                        cGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATc                                                                                                                   <  1:950961/238‑1 (MQ=255)
                                                                                                                          gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGCGTTGATGGAAGCGCTGCTGGCTTTcccc                                                                                                                >  1:123954/1‑237 (MQ=255)
                                                                                                                          gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                                                >  1:221041/1‑239 (MQ=255)
                                                                                                                          gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGGCCGGTCGTGGGGGCGAGCCTTGCGCAAACCGGTGCGGTGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                                                >  1:920161/1‑239 (MQ=255)
                                                                                                                          gTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGGTTGGGCAGCCCGGTTCTTAAGGGGATTAACGAATCCACCAATCAGAATGCAGCAGGGAAGCGTATTGGGTTCGTCGGTCCGCGCGGGGGGGCAGACCCTGCGCAAACCGGTGGGAGGGGGGTGAGGGCGGCGGTGCTGTCTCTcccc                                                                                                                >  2:950961/1‑237 (MQ=255)
                                                                                                                           ttttGAAGGGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTc                                                                                                                <  1:845072/238‑1 (MQ=255)
                                                                                                                           ttttGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCg                                                                                                                                                                              <  1:186548/176‑1 (MQ=255)
                                                                                                                                     ccGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGGGGTTAACGAATCCCCCAATCAGAATGCAGCAGGGAAGCGGTTTGGGGTCCGCGGTCCTAGCGGGGGGGCAGa                                                                                                                                                                    >  2:186548/1‑176 (MQ=255)
                                                                                                                                                      tGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGAGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGc                                                                                    >  1:540392/1‑239 (MQ=255)
                                                                                                                                                             cgcCTTGACATCAGGGTCCGTATGCCCCCTGCTGAAGGCAAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggc                                                                             <  2:123954/238‑1 (MQ=255)
                                                                                                                                                                cATGACATCGTGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGGTTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAAGCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTggcgg                                                                           <  1:598961/238‑1 (MQ=255)
                                                                                                                                                                 aTGACATCAGGGTCCGTATGCCGGCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGTAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCa                                                                         <  1:86540/239‑1 (MQ=255)
                                                                                                                                                                      ctcctggggcGTATGCCGCCTGCGGAAGGCGAAACCGGGGGTCAGGGGGGGGGCAGCCAGGGGCGGAAGGTTATGAACGAATCCACCAAGCAGAATGCAGCAGGGAAGCGTATGGAGGGCGGCGGTCCGAGCGGGGGGGCAGACCGGGCGCAAACCGGGGCGAGGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGggt                                                                    <  2:598081/230‑1 (MQ=255)
                                                                                                                                                                      agctttgTCCGGATGCCGCCTGGTGAAGGCGAAACCGGGGGGCAGGTGTTGGGGCGCCGGGTTCTGAAGGGGATTAACGAATCCACCAATGAGAATGCAGCAGGGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAgggg                                                                     <  2:292729/232‑1 (MQ=255)
                                                                                                                                                                          tGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGGTGGGGTTGATGGCAGCGCTGCTGTCTATCCCCGTGTACGTAGGTTTCCCCTTTTGGGGGGGACTGGCGgggggggggggg                                                                >  2:1084638/1‑237 (MQ=255)
                                                                                                                                                                             tCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGGTGGGCAGCCAGGTTCTTAAGGGGCTTAACCAATTCACCAATCAGAATGCAGCAGTGAAGCGTATTGTGTTCGTCGGACCAGGGGTGGGGGCAGACCTTGCGCAAACCGGGTGGATGGGGTGGATGGCAACGCTGCTGTCTATCCTCGTGTTCTGAGGTTTCCGCTTTGAGTGGGGCCTGGCGGCAGGggtggtagtt                                                             >  2:232291/1‑235 (MQ=255)
                                                                                                                                                                               cGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTTTTg                                                            >  1:908349/1‑238 (MQ=255)
                                                                                                                                                                                gttttccgccTGCCGAAGGCGAAACCGGCGGTTAGGTGTTGGGCAGCCTGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcg                                                          <  2:279719/234‑1 (MQ=255)
                                                                                                                                                                                gTATGCCGGCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcg                                                          <  1:41214/239‑1 (MQ=255)
                                                                                                                                                                                  aTGCCGCCTGCTGAAGGCTGAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTgcgc                                                         <  1:180094/238‑1 (MQ=255)
                                                                                                                                                                                      cgccTTCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGgcg                                                    <  2:221041/239‑1 (MQ=255)
                                                                                                                                                                                       gccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                                              >  1:438094/1‑164 (MQ=255)
                                                                                                                                                                                       gccTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGGATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAgcgc                                                                                                                              <  2:438094/164‑1 (MQ=255)
                                                                                                                                                                                         cttgtgAAGGGGAAACCGGGGGGTAGGTTTTGGGGCGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCac                                                 <  2:920161/235‑1 (MQ=255)
                                                                                                                                                                                              gAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGGCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                                            <  1:23597/239‑1 (MQ=255)
                                                                                                                                                                                              gAAGGCGAAACCGGCGGTCAGGTGGTGGGCAGCCAGGTTCGGAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                                            <  1:870024/239‑1 (MQ=255)
                                                                                                                                                                                              gAAGGCCAACCCGGCGGTCAGGTGTTGGGCAGACAGGTTCTGAAGGTGATTAACGAATCCCCCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGt                                            <  2:1080635/239‑1 (MQ=255)
                                                                                                                                                                                                          gggggTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATGAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt                                <  1:667440/236‑1 (MQ=255)
                                                                                                                                                                                                          ggcggTCAGGTGTGGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt                                <  1:560468/239‑1 (MQ=255)
                                                                                                                                                                                                          ggcggTCAGGGTTTGGGCTGCCGGGTTCTGGAGGGGATTCACGGATCCCCCAATCAGAATGCAGCAGTGAAGCGTAATGAGTTCGTCGGTTCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCt                                <  1:433533/239‑1 (MQ=255)
                                                                                                                                                                                                                     tttggggAAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAAGCAGAATGCAGGAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGGGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTAtttt                       <  2:1059146/234‑1 (MQ=255)
                                                                                                                                                                                                                        tGGGGAGCCGGGTTGTGAAGGTGATTAACGAATCCACCAATCAGAATGCGGCAGTGAACCGTATTGAGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATTGCGTTGATGGCAGCGCTGCTGTCTAGCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCg                   <  2:540392/239‑1 (MQ=255)
                                                                                                                                                                                                                        gtgtcAGCCGGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGGTCGGCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGAGGGCAGCGCTGCTGGCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCg                   <  1:25368/235‑1 (MQ=255)
                                                                                                                                                                                                                          ggCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCt                                                        >  1:696428/1‑200 (MQ=255)
                                                                                                                                                                                                                          ggCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCt                                                        <  2:696428/200‑1 (MQ=255)
                                                                                                                                                                                                                               ccAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGGACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGCGGTTATTGCGCTGGCGCACGACGTTATCATTAGGCGGGGGGTTTTGTGGTGTTTcc            >  2:1003313/1‑239 (MQ=255)
                                                                                                                                                                                                                                    ttCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGGGGTTATTGGGCGGGCGCACGACGTTATACTTACGCTGGGGATTTTGTctgttttcccaatc       >  2:191776/1‑226 (MQ=255)
                                                                                                                                                                                                                                    ttCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTGCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCatat        <  1:958420/238‑1 (MQ=255)
                                                                                                                                                                                                                                       tGAAGGTTATTAACGAATCCACCAATCAGAATGCAGGAGTGAAGCGTATTGGGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCgag    <  1:1084638/239‑1 (MQ=255)
                                                                                                                                                                                                                                          aaGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGCGGGTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAt  >  1:672951/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                          
CGTTATTATGGGCGTGCGCGGCTTTAACTGGGGGCTGGATTTCACCGGTGGTACGGTTATTGAAATTACGCTCGAAAAACCGGCTGAAATTGACGTAATGCGTGATGCATTGCAAAAAGCCGGTTTTGAAGAGCCGATGCTGCAAAACTTTGGTAGCAGCCATGACATCATGGTCCGTATGCCGCCTGCTGAAGGCGAAACCGGCGGTCAGGTGTTGGGCAGCCAGGTTCTGAAGGTGATTAACGAATCCACCAATCAGAATGCAGCAGTGAAGCGTATTGAGTTCGTCGGTCCGAGCGTGGGGGCAGACCTTGCGCAAACCGGTGCGATGGCGTTGATGGCAGCGCTGCTGTCTATCCTCGTGTACGTAGGTTTCCGCTTTGAGTGGCGACTGGCGGCAGGGGTGGTTATTGCGCTGGCGCACGACGTTATCATTACGCTGGGTATTTTGTCGTTATTCCATATCGAGAT  >  NC_000913/429618‑430088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: