BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,156,160	A→G	14.0%	E50G (GAG→GGG)	argB →	acetylglutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,160	0	A	G	14.0%	234.7 / 2.2	109	E50G (GAG→GGG)	argB	acetylglutamate kinase
Reads supporting (aligned to +/- strand): ref base A (40/48); new base G (7/12); total (47/62)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGG  >  NC_000913/4155923‑4156384

cTTACGGAAAGGCGCGGCGGCACAAGGGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAg                                                                                                                                                                                                                                        <  1:354505/239‑1 (MQ=255)

   aCTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCCCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGACTTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCCCGGCGGCGGTTGCGGGGTGGATGa                                                                                                                                                                                                                                         >  1:263859/1‑235 (MQ=255)

            cgcgGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCCCGGCGGCGGGTGCGGGGGGGGCGAGCGGGGGAAAggg                                                                                                                                                                                                                            >  2:1060903/1‑239 (MQ=255)

             gcggggGCACAAGCGGTTCAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAggg                                                                                                                                                                                                                            <  2:143354/238‑1 (MQ=255)

               ggcggcACAAGCGGTACAGTGCGCCAATTTTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCt                                                                                                                                                                                                                          <  1:571933/238‑1 (MQ=255)

                                 gTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGGCACATCAGCGTCCGCTGGGGAT‑‑TGTGCCCGGCGGCGGTTGCGGGGGGGGTGGGCTGTTGAAAGGGCTGGATCTGCCGGGgaaaaag                                                                                                                                                                                                       >  2:1087448/1‑239 (MQ=255)

                                  tGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGGGGGGGATGGGCTGATGAAAGGGCGGAAACTGCGGGGgaaaaaga                                                                                                                                                                                                      >  2:492162/1‑239 (MQ=255)

                                    cgcCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGGGCT‑‑TGTGCACGGCGGCGGTTGCGGGGTGGATTAGCTGATGAAAGGGCTGAACTTTCCGGTgaaaaagaaa                                                                                                                                                                                                    >  1:1035054/1‑239 (MQ=255)

                                    cgcCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTACCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGGGGT‑‑TGTGGCCGGCGGCGGTTGCGTGGGGGATGGGCTGCTGAAAGGGCGGAATCTGCCGGGggaaaggaaa                                                                                                                                                                                                    >  1:479414/1‑239 (MQ=255)

                                       cAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGTCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGGGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGCAAGGGCTGAATCTGCCGGTgcaaaggaaaacc                                                                                                                                                                                                 >  1:884084/1‑237 (MQ=255)

                                       cAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGGGGTGGATGGGCTGATGAAAGGGCGGAATCTGCCGGTGGAAAAGAAAAAc                                                                                                                                                                                                 >  1:472541/1‑239 (MQ=255)

                                       cAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGGGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGTTGAAAAGGCTGAATCTGCCGGTggaaaagaaaaag                                                                                                                                                                                                 >  1:353856/1‑238 (MQ=255)

                                         atatTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGGGAAAAAGAAAAACg                                                                                                                                                                                                >  1:378609/1‑238 (MQ=255)

                                          tatTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACgg                                                                                                                                                                                               >  1:415762/1‑238 (MQ=255)

                                            ttCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGCGGGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGGACGGCGGCGGTTTGTGGGTGGTGGGGCTGTAGAAAGGGCGGAAATTCCCGGGGGAAAAGAAAAACGGccg                                                                                                                                                                                            >  2:583076/1‑238 (MQ=255)

                                             ttgTTTCGGCTTGTCGGAAACGCAGTTCTTTATTTAAGGGTGCAATGATGAAACCATTAATTATCAAACTGGGCGGGGTACTGCTGGATAGTGAAGGGGCGCTGGAACGTCTGGTTAGCTCACTGGGGAATTAGCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTg                                                                                                                                                                                           <  2:270183/237‑1 (MQ=255)

                                                  ttggCTTTGCGGAACCGCGGTTTCTTTTTTAAGGGTGCAATGATGAATCCATTAATTATCAAACGGGGCGGCGTACTGCTGGATAGGGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGt                                                                                                                                                                                      <  2:1003671/237‑1 (MQ=255)

                                                      cTATGCGGAAACGGCGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGAc                                                                                                                                                                                   <  1:51625/238‑1 (MQ=255)

                                                      cTATGCGGAAACGCAGTCTGTTAGTGAAGGGTTAAATGATGAACCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGAc                                                                                                                                                                                   <  1:994984/238‑1 (MQ=255)

                                                       tATGCTGAAACGCAGTCTTTGATTTAAGGGTGCAATGAAGAATCCATTAATTAACAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGAGGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGc                                                                                                                                                                                 <  2:905050/239‑1 (MQ=255)

                                                           cGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGGGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGGGAAAAAGAAAAAAGGGCTGCGGGGGagacccgc                                                                                                                                                                             >  2:442440/1‑232 (MQ=255)

                                                              aaaccgtttcttTTTTTTAAGGGGGTAAGGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCctgctg                                                                                                                                                                           <  2:378609/230‑1 (MQ=255)

                                                               aaCGTAGTTTTTTATTTAAGGTTGCAGTTGTGAATCCTTTAATTATCAACCGGGGCGGCGGACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCCCGGGTTAATTATCGTGAGTCACACCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGAt                                                                                                                                                                         <  2:63407/239‑1 (MQ=255)

                                                               aaCGCAGTCTTTTATTTTAGGGTACAATGAGTAATCCATTTAGTAGCAAACTGGGCGGCGTGCTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGAt                                                                                                                                                                         <  2:665644/239‑1 (MQ=255)

                                                               aaCGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATGAGCAAACGGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGAt                                                                                                                                                                         <  1:367874/239‑1 (MQ=255)

                                                                aCGCAGTCGTGTATTGAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTTCTGCTGGAGAGTGAAGGGGCGCTGGAACTTCTGGTTAGGGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGAt                                                                                                                                                                         <  1:508948/238‑1 (MQ=255)

                                                                    aGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGa                                                                                                                                                                     <  1:583076/238‑1 (MQ=255)

                                                                      gtttttttttAAGGGTGCAATTATGAAACCATTAATTATCAAACTGGGCGGGTGACTGGTGGATAGGGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAg                                                                                                                                                                  <  2:352863/232‑1 (MQ=255)

                                                                             tttAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTTAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATa                                                                                                                                                                   <  1:126360/231‑1 (MQ=255)

                                                                                aaGGGTGCAATGAGGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCAATGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCAc                                                                                                                                                        <  1:171674/239‑1 (MQ=255)

                                                                                  gggTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGCTGAAAGGGCTGAATCTGCCGGGGAGAAAGAAAAACGGCCTGCGGGGGAAGCCGGCTGATCAGTTAGGAATTAACACCg                                                                                                                                                      >  2:171674/1‑239 (MQ=255)

                                                                                  gggTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGGGACGCCTGCTGATCAGATAGACATTATCAc                                                                                                                                                        >  1:352863/1‑237 (MQ=255)

                                                                                  gggTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCGGCGGGGGAGGCCTGCTGATAAAGAAGACAATATCACCg                                                                                                                                                      >  2:983263/1‑239 (MQ=255)

                                                                                    gTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGGAAAAGAAAAACGGCCTGCGGGTGCAGCCTGCGGGTGGGCTAGACATTATCACCggc                                                                                                                                                    >  1:241573/1‑238 (MQ=255)

                                                                                    gTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGGGACGCCTGCTGATACGATATACATTATCACCGGa                                                                                                                                                    >  1:199064/1‑239 (MQ=255)

                                                                                     tGCAATTATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGa                                                                                                                                                                     <  1:1060526/221‑1 (MQ=255)

                                                                                      ttaaggttgaAACCATTAATTTTAAAATTGGGGGGCGTACTGCTGGATAGTGAAGGGGCGTTGGAACGTCTTGTTAGCGCACTGGTGAATTATCGGGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAg                                                                                                                                                   <  2:479414/231‑1 (MQ=255)

                                                                                       cactgatgaATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAATGAAGAGGCGCTGGAACGTCTGTTTAGCGCCCTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGGGGATGAGCTGGTGAAAGGGCGGAACATGCCGGTGGAAAAGAAAAACGGCCTGCGGGGGGTGCCTGCAGATCAGaaaaaaaatataaacagggg                                                                                                                                                  >  2:500440/4‑219 (MQ=255)

                                                                                       cAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCCCGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAAGGGCCTGCGGGGGAGGCCTGCTGATCAGAAAAACATTAACAcccgtc                                                                                                                                                   >  2:126360/1‑233 (MQ=255)

                                                                                       cAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGGGGAAACGAAAAAAGGCCTCGGGGTGACGCCTGCGGATCCGATGAAAATTAACACCGGAga                                                                                                                                                  >  2:877596/1‑237 (MQ=255)

                                                                                       cAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGGGAAAAAGAAAAACGGCCTGCGGGTGCCGCCCGCTGATCAAAAAGaaaaaaaaacaagggaa                                                                                                                                                 >  2:101927/1‑223 (MQ=255)

                                                                                        aatgtggaAACCTTTAATTGTCAAACTGGGGGGTGTTCTGGTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCCCTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCa                                                                                                                                                 <  2:472541/232‑1 (MQ=255)

                                                                                           gatgaACCCTTTTATTATTAATCGGGGGGGCGTACTGTTGTGTGTGGAAGAGGCGTTGGAACGTTTGTGTATCGCACTGGTGAATTATCGTGAGTCACAGCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTg                                                                                                                                              <  2:242984/238‑1 (MQ=255)

                                                                                               aaTCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGATTGTGTGCACGGCGGCGGTTGCGTGGTGGATGAGCGGATGAAAGGGCTGAATCTGCCGGGGAAAAAGAAAAACGGCCTGCGGGTGAGCCCTGCTGATCAGATAAAAAATAACACCGGAGAACTgggg                                                                                                                                           >  2:333846/1‑237 (MQ=255)

                                                                                               aaTCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATTTGCCGGGGAAAAAAGAAAAAGGCCTCCGGGGGACGCCTGCTGATCAGATAGAAAATATAAACGGGGACcgggc‑ggg                                                                                                                                        >  2:477041/1‑239 (MQ=255)

                                                                                               aaTCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGGGGATGAGCTGATGAAAGGGCTGAATCTGCCGGGGAAAAAGAAAAAAGGCCGGCGGGGGAAGCCTGCTGGTCAGATAGAAAtaaaccgggttgttaaaaaaa                                                                                                                                          >  2:1060526/1‑218 (MQ=255)

                                                                                                   cATTAATTATGAAACGGGGCGGGGTACGTCGGGATAGTGAAGAGGCGTTGGAACGTCTGTTTAGCGGACTGGTGAATTACCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑g                                                                                                                                  <  1:716916/239‑1 (MQ=255)

                                                                                                   cATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGGCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑g                                                                                                                                  <  1:751936/239‑1 (MQ=255)

                                                                                                    attaattaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGGGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGACCAGATAGACATTATCACCGGAGAACTGGC‑GGG‑GAC‑g                                                                                                                                  >  1:984964/1‑238 (MQ=255)

                                                                                                     tgaagtaTCAAACTGGGCGGGTGTGTGCGGGATAGTGAAGAGGCGCTGGAACGTCTGTGTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑Gc                                                                                                                               <  1:655548/234‑1 (MQ=255)

                                                                                                      taattaTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTTTAACCGGCGCACTGGC‑GGG‑aagcgga                                                                                                                                >  1:1060853/1‑234 (MQ=255)

                                                                                                           aTCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCCCTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGGGGCTGGGCTGATGAAAGGGCTGAACCTGCCGGGGAACAAGAAAACCGGCCTGCGGGGGACGCACGCTGATCCAATGGGCATTATAACCGGGGCCCTGGC‑GGG‑GAC‑G‑GCC‑Aaaaa                                                                                                                        >  2:655548/1‑239 (MQ=255)

                                                                                                            tCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGGGACGCCTGCTGATCAGATAGACATTATAACCGGAGCACTGGC‑GGG‑AAC‑G‑GCC‑aaaaaa                                                                                                                       >  1:771071/1‑239 (MQ=255)

                                                                                                             cAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATaaaa                                                                                                                      <  2:144306/239‑1 (MQ=255)

                                                                                                             cAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATaaaa                                                                                                                      <  1:877596/239‑1 (MQ=255)

                                                                                                             cAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCGGGAACGTCTTGTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATaaaa                                                                                                                      <  1:255965/239‑1 (MQ=255)

                                                                                                                    ggcggcGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATAACAGGAGCACTGGC‑GGG‑GAC‑G‑GCC‑AATAAAA‑ACCCGtt                                                                                                              >  1:783057/1‑239 (MQ=255)

                                                                                                                         cgTCCTTCTGGATAGGGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCa                                                                                                          <  1:346786/238‑1 (MQ=255)

                                                                                                                               ggtgGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCga                                                                                                   <  1:706920/237‑1 (MQ=255)

                                                                                                                                  ggATAGTGAAGGGGCGGTGGAACGTCTGGTTAGCGCACTGGTTATTTATCGTGAGGCACATCAGCGTCAGCGTGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGGGCTGAGGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCgaag                                                                                                 <  1:508234/238‑1 (MQ=255)

                                                                                                                                  ggAAAGGGAAGGGGCGTTGGAACGTCTGTTCAGCGACATGGGGAATTATCGTGAGTCAACGCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCAGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCgaag                                                                                                 <  1:387806/238‑1 (MQ=255)

                                                                                                                                     tAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACCTTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCG‑AATAAAA‑accatgtggccagggggggaaaaa                                                                                             >  1:126389/1‑218 (MQ=255)

                                                                                                                                        tGAAGGGGCGGTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑ggg                                                                                                                                        <  1:724629/198‑1 (MQ=255)

                                                                                                                                        tGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGCAAAACGGCCTGCGGGGGACGCCTGCTGATCAGATAGACATTATCACCGGAGCCATGGC‑GGG‑AACAG‑GCACAAAAAAA‑CCTTTTTGGGGGGGGGGAAAAAACa                                                                                            >  2:361183/1‑239 (MQ=255)

                                                                                                                                          aaGAGGCGCTGGAACGTCTGCTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGGGGTGGATGAGCTGATGAAAGGGCTGAATCCGCCGGGGAAAAAGAAAAACGGCCTGCGGGGGACGCCTGCTGGTCAGATAGACAATAACACCGCAGCCCGGGC‑GGGTAAC‑G‑CCA‑AA‑ACAA‑ACCAGTTGGCCTgggtgcacacaacacca                                                                                        >  2:21506/1‑225 (MQ=255)

                                                                                                                                             agGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGCGGGG‑AAG‑G‑GCA‑ATTAAAA‑CAC‑GTTGGCATggggggcagaaaacgccgtt                                                                                     >  1:843362/1‑222 (MQ=255)

                                                                                                                                             agGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCC‑AATAAAA‑ACCCGTTGGCCTGGGCGGAGAAAAAACAG‑aat                                                                                    >  1:140659/1‑237 (MQ=255)

                                                                                                                                                              tttAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑g                                                                                                                                  <  1:613965/180‑1 (MQ=255)

                                                                                                                                                              tttAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGGCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGt                                                                            <  1:363392/230‑1 (MQ=255)

                                                                                                                                                                   cgcACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGCCGCCGGCTGATCAGATAGACATTATCACCGGAGCACGGGC‑GGG‑AAA‑GCCCA‑AAAAAAC‑CCCCGTGTGCAGGGGCG‑AGGAACACCAGAATTCGGGCCGAAGGGGTGTTtctc                                                               >  2:630827/1‑239 (MQ=255)

                                                                                                                                                                    ggcagggTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCg                                                              <  1:1001367/233‑1 (MQ=255)

                                                                                                                                                                        tGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑GAC‑G‑GCC‑AATAAAACCCCTGTT‑GCATTGGCGCAGAAACATAAG‑ATTTCGGGCGGAGGGTTGTTTTTCGGTggt                                                         >  1:915622/1‑237 (MQ=255)

                                                                                                                                                                        tGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCC‑AATAAAA‑CCCTGTTTGCCTGGGGGAGGAAAAATCCA‑ATTGGGGGCGGAGGGTTTTTTTTCGGGGag                                                         >  1:121222/1‑238 (MQ=255)

                                                                                                                                                                              aTTATCGGGAGTCACTTCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGa                                                    <  1:253969/238‑1 (MQ=255)

                                                                                                                                                                                    gTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGa                                                                                                                                                                     <  1:659337/126‑1 (MQ=255)

                                                                                                                                                                                    gTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGa                                                                                                                                                                     >  2:659337/1‑126 (MQ=255)

                                                                                                                                                                                    gTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATAACCGGAGCACTGGC‑GGG‑AAG‑G‑GGA‑AAAAAAA‑ACCCGTTGGGCGGGGGGGAGAAAAAACAG‑AATGTGGCCGGAGggttgttttgtggggggggcggccaggca                                             >  2:1001367/1‑218 (MQ=255)

                                                                                                                                                                                         tCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCt                                                                                                                                                                                            <  2:418650/98‑1 (MQ=255)

                                                                                                                                                                                             ataggggTCCTCTGGTGAT‑‑TGTGCACGGGGGGGGTTGCGTGGTGGAGGGGGGGATGAAAGGGCTGAATCTGACGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AAAAAAA‑CCCTGTTGGCATGGGCGAAGAAACAGCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGAcc                                    <  2:238654/233‑1 (MQ=255)

                                                                                                                                                                                               ccgggTCCGGTGGTGTT‑‑TGTGCCCGGCGGGGGGTGCGTGGGGGATGGGGTGATGAAAGGGCTGAATCTGCCGGGGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCa                                  <  2:1035054/235‑1 (MQ=255)

                                                                                                                                                                                                   gTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGc                                <  1:235854/237‑1 (MQ=255)

                                                                                                                                                                                                    tCCGTTGGGGGT‑‑TGTGCCCGGGGGGGGTTGCGGGGGGGTTGAGGTGAGGAAAGGGCTAAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCtt                              <  2:1075188/238‑1 (MQ=255)

                                                                                                                                                                                                      cGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑AGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGGCAAAGGAACCCAGCTtgtt                           >  1:372266/1‑237 (MQ=255)

                                                                                                                                                                                                       ggtGGTGTT‑‑TGGGCACGGCGGGGGTGGGGTGGGGGATGGGCTGTTGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTtgatg                          <  2:771071/237‑1 (MQ=255)

                                                                                                                                                                                                       ggtGGGGAT‑‑TGTGCAAGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTtgatg                          <  1:1087448/237‑1 (MQ=255)

                                                                                                                                                                                                       gCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTtgatg                          <  1:696711/239‑1 (MQ=255)

                                                                                                                                                                                                       gCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTtgatg                          <  1:934433/239‑1 (MQ=255)

                                                                                                                                                                                                       gCTGGTGAT‑‑TGGTCCCGGGGGCGGTTGCGGGGGGGATGGGAGTAGGAAAGGGCTGAAATTGCCGGTGAAAAAAAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTtgatg                          <  2:783057/239‑1 (MQ=255)

                                                                                                                                                                                                       gCTGGGTAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTtgatg                          <  1:515188/239‑1 (MQ=255)

                                                                                                                                                                                                        cTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGTCATGGGGGAAGAAACATCAG‑ATTGCGGCCGTAGGGTTGTTTCTCGGTGACGGCGGCAGCGTCAAAGGGACCCCGCTtgatga                         >  1:1004600/1‑239 (MQ=255)

                                                                                                                                                                                                          ggtgattgtgtgCACGGCGGCGGTTGCGTGGTGGATGGGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTtgatg                          <  1:333846/230‑1 (MQ=255)

                                                                                                                                                                                                                atttggccaggcggcggTGGGGTGGTGGATGGGATGAGGAAAGGGCTGAAGCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCAGGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGt                    <  2:702536/229‑1 (MQ=255)

                                                                                                                                                                                                                   tggCACGGCGGAGTTTGCGTGGTGTATGGGCTGATGAAAGGGCTGAAGCTGCCGGTGAAAAAGAAAAACGGCCTGGGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAgg                <  2:241573/237‑1 (MQ=255)

                                                                                                                                                                                                                   gtgCTCGGGGGGGGGTTCCTGGTGGGTTGGCTGATGAAAGGGCTGAATTTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCAGCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTTGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTTACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAgg                <  1:387901/239‑1 (MQ=255)

                                                                                                                                                                                                                   gtgCCCGGGGGGGGTTGGGTGGTGGGGGGGGTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑AGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAgg                <  2:372266/239‑1 (MQ=255)

                                                                                                                                                                                                                   gtgCACGGCGGGGGTTGGGGGGTGGATGCGGTGGTGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCGGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAgg                <  2:121222/239‑1 (MQ=255)

                                                                                                                                                                                                                   gtgCACGCCGGGGGTTGCGTGGTGGATGGGTGGATAAAAGGGCTAAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAgg                <  1:477041/239‑1 (MQ=255)

                                                                                                                                                                                                                   gggcaggggggggtttGGGTGGGGGATGAGTGGAGGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAgg                <  2:140659/225‑1 (MQ=255)

                                                                                                                                                                                                                               ggTTGCGTGGTGGATGGGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGAc     <  1:293376/238‑1 (MQ=255)

                                                                                                                                                                                                                                 ttGCGTGGTGGATGTGCTTGTGAAAGGGTTGATTCTGACGGTGAAAAAGAAAACCGGCCTGCGGGTGACTACTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTgg  <  2:894196/239‑1 (MQ=255)

CTTACTGAAAGGCGCGGCGGCACAAGCGGTACAGTGCGCCAATATTCGTTTCGGCTATGCGGAAACGCAGTCTCTTATTTAAGGGTGCAATGATGAATCCATTAATTATCAAACTGGGCGGCGTACTGCTGGATAGTGAAGAGGCGCTGGAACGTCTGTTTAGCGCACTGGTGAATTATCGTGAGTCACATCAGCGTCCGCTGGTGAT‑‑TGTGCACGGCGGCGGTTGCGTGGTGGATGAGCTGATGAAAGGGCTGAATCTGCCGGTGAAAAAGAAAAACGGCCTGCGGGTGACGCCTGCTGATCAGATAGACATTATCACCGGAGCACTGGC‑GGG‑AAC‑G‑GCA‑AATAAAA‑CCCTGTTGGCATGGGCGAAGAAACATCAG‑ATTGCGGCCGTAGGTTTGTTTCTCGGTGACGGCGACAGCGTCAAAGTGACCCAGCTTGATGAAGAGTTAGGTCATGTTGGACTGG  >  NC_000913/4155923‑4156384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`