Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,377,377 T→A 16.1% Y116F (TAT→TTT)  blc ← outer membrane lipoprotein cell division and growth lipocalin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,377,3770TA16.1% 174.9 / 4.1 73Y116F (TAT→TTT) blcouter membrane lipoprotein cell division and growth lipocalin
Reads supporting (aligned to +/- strand):  ref base T (56/4);  new base A (2/11);  total (58/15)
Fisher's exact test for biased strand distribution p-value = 2.65e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTT  >  NC_000913/4377142‑4377614
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
tCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATaa                                                                                                                                                                                                                                                >  1:970191/1‑238 (MQ=255)
    gCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGGGCAATAACGGTATAACcccc                                                                                                                                                                                                                                          >  1:1028004/1‑239 (MQ=255)
         aaTCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAACGCATGGCGGAATTCCCGTACGAGTGCAATAACGTTATAAACGCCATAgc                                                                                                                                                                                                                                     >  1:197388/1‑238 (MQ=255)
              tATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCCTAGaaact                                                                                                                                                                                                                                 >  1:833680/1‑236 (MQ=255)
              tATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGGTCGGGAGAGATTCCACAGGTAGTCCCGGTCCGGGCCGCAAACCAGCGACTGCCGGTATTTCGGCACGGGGGCAACAACGGTAAAACCgacttctttaaac                                                                                                                                                                                                                                >  2:598297/1‑227 (MQ=255)
                     gATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGGCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTGTAACCGCCATAGAATGGAACCAAGa                                                                                                                                                                                                                         >  1:434614/1‑239 (MQ=255)
                        tattGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAACTGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGGATTCCCGATCCAGGGCAATAACGTTATAACCCCCCAAGAAAGGACCaaaaaaga                                                                                                                                                                                                                      >  2:672457/1‑237 (MQ=255)
                        tattGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATg                                                                                                                                                                                                                      >  1:461609/1‑239 (MQ=255)
                             gTCTGGAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGGATTCCCGCTCGGGTGCAATAACGTTAAAACCCCCCTAGAAAAGACCaaaaaagaaaact                                                                                                                                                                                                                 >  2:780830/1‑232 (MQ=255)
                             gTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCAGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGGAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTTTAACCCCAATAGAAAGGACCAAAGCATGgcagt                                                                                                                                                                                                                 >  1:432178/1‑237 (MQ=255)
                                tGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTc                                                                                                                                                                                                              >  1:621373/1‑239 (MQ=255)
                                          cactcactAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCAGTCCGGGCCGCCAACCAGCGCATGGCGGTATTCCCGACCGAGGGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGAAACTTTaagcccagccc                                                                                                                                                                                                   >  1:532882/1‑237 (MQ=255)
                                               actaactaCCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCACCGCGAGtt                                                                                                                                                                                              >  1:266274/1‑239 (MQ=255)
                                                       ccAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAATAATGACACCTTCAGCGAAACGCGAGTTGGGGCGcc                                                                                                                                                                                     >  1:1048964/1‑239 (MQ=255)
                                                         aGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCCGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAAGAACACTTTCAGCGAAGCGCGGGTTGTGCGcccgg                                                                                                                                                                                  >  2:211455/1‑236 (MQ=255)
                                                         aGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACAATTTAAGCCCAACCCGAGTTgggggccggg                                                                                                                                                                                   >  2:226461/1‑231 (MQ=255)
                                                           gctgctGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCACTAACGTTATAACCGCCATAGAAAGGACCAAAGAATGAACCTTTAAGCGCAGCGCGAGTTGGTGGGCCGGTa                                                                                                                                                                                 >  1:322331/1‑239 (MQ=255)
                                                           gctgctGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTa                                                                                                                                                                                 >  1:318316/1‑239 (MQ=255)
                                                           gctgctGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCACCGCGAGTTGGTGCGCCGGTa                                                                                                                                                                                 >  1:212530/1‑239 (MQ=255)
                                                           gctgctGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAACGCGAGTTGGTGCGCCggga                                                                                                                                                                                 >  1:673466/1‑237 (MQ=255)
                                                           gctgctGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGCGCAATAACGTTATAACCGCCATAGAAAGGACCACAGAATGACACTTTCAGCGAAGCGCGAGTTGGTGCGCCGGTa                                                                                                                                                                                 >  1:212975/1‑239 (MQ=255)
                                                               ctgTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAga                                                                                                                                                                             >  1:352352/1‑238 (MQ=255)
                                                                tgTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCCAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTCTAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAACGCAGCGCGAGTTGGTGCGCCGGTAAAGt                                                                                                                                                                             >  1:861026/1‑238 (MQ=255)
                                                                    cccAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCt                                                                                                                                                                        >  1:800419/1‑239 (MQ=255)
                                                                         ataaatTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTc                                                                                                                                                                     >  1:199103/1‑237 (MQ=255)
                                                                                       aTCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCtgctgc                                                                                                                                                      >  1:618707/1‑238 (MQ=255)
                                                                                       aTCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCCCACCGCGAGTTGATACGCCGGTACAGTACGCTTTCCCTTCACTCTGCTGcc                                                                                                                                                     >  1:959057/1‑239 (MQ=255)
                                                                                       aTCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCAGGTGCACTAACGTTATAACAGCCATAGACAGGCACCAAGAATGATACTTTATGCGCTGCGCGGTGGTGTGCCCCGGGAAAAAACCGTTTCCCTTccccccccagcc                                                                                                                                                     >  2:27188/1‑228 (MQ=255)
                                                                                                 tCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGCTCGAGTGAAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATAACACTTTCATCGCAACGCGAGTTTGGGCGCCGGAAAAGAACGCTTTACATTCCAACCGATGCCACATTca                                                                                                                                              >  2:148553/1‑235 (MQ=255)
                                                                                                 tCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGGGCGCCGGTAAAGGACGCTTTCCCTTCACTCTGCTGCCACATTcc                                                                                                                                              >  1:36601/1‑236 (MQ=255)
                                                                                                 tCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACAATTTCAGCGCAGCGCGAGTTGGTGAGCCGGTAAAGTACGCTTTCCCTTCAATCTGCTGCCACATTcc                                                                                                                                              >  1:96750/1‑236 (MQ=255)
                                                                                                 tCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAAGACCAAAGAATGGCACTTTCAACGAAGCGCGAGTTGGTGCGCCGGTAAAGAAAGCTTTTCCTTCCCTCTGCTGCCACATTcc                                                                                                                                              >  1:533011/1‑236 (MQ=255)
                                                                                                 tCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGGATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGCGCGCCGGTAAAAAACGCTTTCCCTCCCCCCTCCCGCCACATTcc                                                                                                                                              >  2:933576/1‑236 (MQ=255)
                                                                                                                 ccAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGCGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCCGCCCCATTCCCCTGTCAGGGTTAAAGCCt                                                                                                                           >  2:8644/1‑239 (MQ=255)
                                                                                                                                tCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGGGGGCCGGTAAAGAACGCTTTCCCTTACCCCTCCTGCCCCATTCCCCTGCACGGGTTTAAGCCTTTATTAAAGAAAtta                                                                                                            >  2:145255/1‑238 (MQ=255)
                                                                                                                                   cTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTcc                                                                                                                                                                    >  1:152203/1‑180 (MQ=255)
                                                                                                                                   cTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTTTCAGGGTTTTAGc                                                                                                                             >  1:977951/1‑219 (MQ=255)
                                                                                                                                   cTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAg                                                                                                          >  1:71893/1‑238 (MQ=255)
                                                                                                                                     tCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGgc                                                                                                        >  1:556157/1‑238 (MQ=255)
                                                                                                                                                  gtgcggTGGGGGGGATTAGCCAGGGGTTGTCGCGGCCCGCCCCCAAACCCAGGGCAGGGGTGTACCCCGGATGGAATCAAATAAGTGTAAAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACg                                                                                           <  2:322331/233‑1 (MQ=255)
                                                                                                                                                   tgggggtggggAGAGATACCCAGGGGGAGGCGCGGTCCGGCCAGCAAACCAGCGGATGGCGGTATCCCCGAGTGATAAAAATATGGTTAAAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACg                                                                                           <  1:780830/230‑1 (MQ=255)
                                                                                                                                                      cgggCGGGAGAGTACCCACGGGTAGGCGCGTCCGGGCCCACAACCCGGCGCGGGGCGTTATCCCCGAGCGAGTGCAATACCGTTAAAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAgg                                                                                       <  1:8644/235‑1 (MQ=255)
                                                                                                                                                          cGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCCAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGGACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGAAATTCAGGCCGCCCTCCTCAACCAGGCGGTa                                                                                  >  2:688590/1‑239 (MQ=255)
                                                                                                                                                                agTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAACGCAGCGCGAGTTGGTGCGCCGGTAAAGTAAGCTTTCCCTTCCCTCTGCTGCCACATTCCTCTTTCAGGGGTATAGCCTTTATTAATGAACTTCAGGCCGCCGGCCTCCACCAGGCTGGttgttgg                                                                            >  2:818988/1‑238 (MQ=255)
                                                                                                                                                                   tacacagagggaggcggggccggggCCAAAACCCGGCGGAGGGCGGTATCCCCGTTCGGTGACAATAAGTTTAAAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCgg                                                                          <  1:145255/217‑1 (MQ=255)
                                                                                                                                                                    ctccagaggctgcgggggTCCGCGCCAAAAACCGGCGCATGGCGGTATCCCAGGTGGGGTTAAATGATGTAATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCgg                                                                          <  1:264620/222‑1 (MQ=255)
                                                                                                                                                                            gTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACtttt                                                                  >  1:954353/1‑238 (MQ=255)
                                                                                                                                                                            gTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGAATTCCCGATCCAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGAGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTCTAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACtttt                                                                  >  1:215100/1‑238 (MQ=255)
                                                                                                                                                                              aGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGCCCTCACGCAGGCTGTATGTTGCGGTGACTTTTTcc                                                               >  1:172461/1‑239 (MQ=255)
                                                                                                                                                                               gTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGCCATTCAgg                                                                                                         >  1:19796/1‑196 (MQ=255)
                                                                                                                                                                               gTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACCTTCAGGCCCCCGTCATCACCCAGGCTGTATGCTgtcccttaaacaaatc                                                              >  2:60084/1‑224 (MQ=255)
                                                                                                                                                                               gTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTAATCACCCGGGCCGGATGTTGCGGGGACTTTTTcaa                                                              >  2:580447/1‑237 (MQ=255)
                                                                                                                                                                               gTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGCCATCACGCAGGCTGTATGTTGGGGGGACTTTTTCCa                                                              >  2:677790/1‑239 (MQ=255)
                                                                                                                                                                                tCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTATTGACATTCAGGCCGCCGTCATCACGCAGGCTGTCTGTTGCGGGGCCTTTTTCCa                                                              >  1:428102/1‑238 (MQ=255)
                                                                                                                                                                                          gggctccacaCCAACGCAGGGGGGTAATCCCGCGTGAGGCCAATAAAGTTTAAAACCGGCATAGAAAGGACCAAAGAATGACCCTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGtt                                                     <  1:516154/230‑1 (MQ=255)
                                                                                                                                                                                                 aaaCCCGCGCGTGGGTGTATTCCCAGAGCGGAGCAAATAAGTTAAAACCCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCg                                              <  1:226461/239‑1 (MQ=255)
                                                                                                                                                                                                 aaaCCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCgg                                             >  1:649600/1‑239 (MQ=255)
                                                                                                                                                                                                        cgcATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCTGCCGTAATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCaa                                      >  1:811083/1‑239 (MQ=255)
                                                                                                                                                                                                             ggCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGGTGTATGTTTGGGGTACTTTTTCCAGTCCCACTTCAAAACGGGGGTCAAAAggg                                 >  2:439648/1‑239 (MQ=255)
                                                                                                                                                                                                                ggttttccaggcgcGAGTGAAAAACCGTAATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACggg                                <  1:818988/225‑1 (MQ=255)
                                                                                                                                                                                                                gcgatatccccggtgggttcaaaTAACGTTGAAGCCGCCATAGAAAGGACCAACGAATGACCCTTTCAGCGCAGCGCGAGTTGGTGCGCTGGTAAAGTTCGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACggg                                <  1:528512/218‑1 (MQ=255)
                                                                                                                                                                                                                ctgtctgccgggttcggcgCAAAAACGTTAACACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACggg                                <  1:933576/220‑1 (MQ=255)
                                                                                                                                                                                                                atttcccccGACGGAGTGAAATAAGTTAATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGc                               <  1:672457/232‑1 (MQ=255)
                                                                                                                                                                                                                 ggataccccagtcggggtaacaaaaCGTAAAACCCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACggg                                <  1:439648/215‑1 (MQ=255)
                                                                                                                                                                                                                  tATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAACCGGGCaa                             >  1:875937/1‑238 (MQ=255)
                                                                                                                                                                                                                  tATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGGATTTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCaa                             >  1:1039414/1‑238 (MQ=255)
                                                                                                                                                                                                                        cccgtcggtgggaaggtgggTTATATCCGGAATAGAAAAGACCAAAGAATGACACTCTCGGCGCAGGGCGAGTTGGTGGGCAGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCa                        <  2:959057/220‑1 (MQ=255)
                                                                                                                                                                                                                             cGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAg                  >  1:803052/1‑239 (MQ=255)
                                                                                                                                                                                                                               aGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTaaa                                                                                                                                                                               >  1:94758/1‑80 (MQ=255)
                                                                                                                                                                                                                                 tGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTa               >  1:37132/1‑238 (MQ=255)
                                                                                                                                                                                                                                  tcaaaTAACGTTTAACCCGACATAAGAAGGACCAAAGAATGACCCGTTCAGCGCAGCGAGAGTTGGGTCGCCGGTAAAGTCCGCTTTCCCTGCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTAc              <  2:1048964/236‑1 (MQ=255)
                                                                                                                                                                                                                                  catatccctgTTAAACCCCCCCTAGAAATGCCCAAAGAATGCCTCTTTCACGCGAGCCCGAGTTGGTGCCCCGTGAAAGTTCGCGTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTAc              <  2:557643/230‑1 (MQ=255)
                                                                                                                                                                                                                                             ttATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATa                      >  1:439582/1‑221 (MQ=255)
                                                                                                                                                                                                                                               ataACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGCATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGCGATAACGtt  >  1:817721/1‑239 (MQ=255)
                                                                                                                                                                                                                                                taACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATa                      >  1:321660/1‑218 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                
TCTGGCGTTAATCGTATTGGGGATTATTGGTCTGAAACTCAGCACTCACTAACTACCAGGCTGCTGTACCCAAATAAATTTACTGACATCAAACCCTTCCCGGGTCGCGACTGCCAGCATCTCCTGTTTCACTTCGTCAGAAATGGTTGGCGTGCGGGAGAGTATCCACAGGTAGTCGCGGTCCGGGCCGCAAACCAGCGCATGGCGGTATTCCCGATCGAGTGCAATAACGTTATAACCGCCATAGAAAGGACCAAAGAATGACACTTTCAGCGCAGCGCGAGTTGGTGCGCCGGTAAAGTACGCTTTCCCTTCACTCTGCTGCCACATTCCTCTGTCAGGGTTATAGCCTTTATTAATGACATTCAGGCCGCCGTCATCACGCAGGCTGTATGTTGCGGTGACTTTTTCCAGTCCACGTTCAAAGCGGTGATCAAAACGGGCAATCTCATACCAGGTACCGAGATAACGTT  >  NC_000913/4377142‑4377614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: