| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 489,597 | T→G | 13.2% | V1021G (GTG→GGG) | mscK → | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 489,597 | 0 | T | G | 13.2% | 269.0 / 5.4 | 118 | V1021G (GTG→GGG) | mscK | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Reads supporting (aligned to +/- strand): ref base T (43/57); new base G (14/4); total (57/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 118 total aligned reads displayed.
TTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAA > NC_000913/489378‑489831 | ggCGATACGGTAACCATTGGTAGCTTCGCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa < 1:584079/239‑1 (MQ=255) ggttttcGGTAACCTTTGGTGCCTTCCCGGGGAACGTAAGGTAGATCCTGTTTCGGGCGACAACGATTACCGATTTTGATCGCAAAGAAGTGATGATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAg < 2:754428/233‑1 (MQ=255) gggATGCGGTAACCATTTGGAGCTTCTCGGAGGCGGTAAGTTAGATCCGTAGTCGTGCGAAAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCATCTGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGGCTGAg < 2:361841/237‑1 (MQ=255) gCGATACGGGAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTAATCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCAACCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa < 1:61737/238‑1 (MQ=255) tgtatggTACCCTTTGGTGTCTTCTCGGGGGCGGTAAGGAAGATCCGTATTCGGTCGACAACGCTTACCGATTTCGATCGCCAAGAAGTGAACATCCCGAACAAAGCC‑TTTGTTGCCGAGCGTCTGATCATCTGGTCGTTGACTGACATTACTACGCGTCTGGTGATCCGTTTCGGCGTGGCCTATGGCCCCGATCTGGAAAAAGTGCGTAAAGTGTTACTTAAGGCGGCGACTGAGCa < 2:901228/234‑1 (MQ=255) aCGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTAAAGGCGGCGACTggggcccc > 1:1021285/1‑232 (MQ=255) ggTAACCATTGGTAGCTGCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑aa < 1:721457/239‑1 (MQ=255) aaCCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAggg > 1:765442/1‑239 (MQ=255) aCCATTGGTAGCTTCTCGGTGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑ATTGTTACCTAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACCCGTCTGGCGCTCAGTCTCGGCGTGGCCATCGGGCCCGTCATGGGACAAGAGCGGAAACGTCTACTGAAGGCGGGACCTGAGCAccccacggg > 2:219839/1‑233 (MQ=255) ccATTGGGAGCTTCGCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTg < 1:212182/239‑1 (MQ=255) tGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCTTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGCCTGAGAACCC‑AAGGGAGGTGc > 1:178074/1‑239 (MQ=255) tGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCCCCGATCTGGGAAAAGGGGGTAAAGGGTTACTGAAGGCGGGGACGGGGGACCC‑AAggggagggc > 2:442352/1‑233 (MQ=255) ggTAGCTTTCCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCa < 1:977838/239‑1 (MQ=255) ggTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCCCGGCGGGGCCTATGGCCCCGCTCTGGTAAAAGGGGGTAAATGGTTACCGAAGGGGGGCCATGAACACCCAAAGGGTgtgac > 2:507669/1‑235 (MQ=255) ttCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTCTGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGAAcc > 1:874145/1‑239 (MQ=255) ttCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAAcc > 1:314996/1‑239 (MQ=255) tccGGGGACGGTAAATAAGATCCGTATTCGTGCGAAAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCaa < 2:314996/237‑1 (MQ=255) aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGGGTTACTGAAGGCGGGGCCTGAGCACCC‑AAGGAGGATGCCAGAAACAAAGGCGGAAGGCTTCTTTAc > 2:504411/1‑239 (MQ=255) aTCCGTATTCGTGCGACAACGATCACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATTTGGAAAAAGGGCGCAAAGTGTTAATGAAGGGGGCGACTGAGCACCC‑AAGGAGGAGTCACGAAACAAGGCCGGAAgcaaacatgaa > 2:891520/1‑229 (MQ=255) tCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGACCCAATGCCGGAACTCTTCTTTACg > 1:915508/1‑239 (MQ=255) ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGACAAGATGCGTAAAGGGTTACTGAAGGCGGCGACTGAGAACCC‑AAGGGGGAGGAAGGAACCACTGCCGGAAGTCTTCTTTACgg > 1:574181/1‑239 (MQ=255) ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGGGCCTGAGCACCC‑AAGGGGGATGCAAGAACCAATGACGGaaagattttttaggg > 2:389561/1‑226 (MQ=255) ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGCTTTCTTTACg > 1:421261/1‑238 (MQ=255) ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGGGTTACTTAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCAAGAACCAAGACAGGAAGACTTCTTTAAgg > 2:342279/1‑239 (MQ=255) tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCAt > 1:52222/1‑239 (MQ=255) tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGACGTTGACTGACACTACTACGCGTCTGGAGATCCGTCTCGGCTTGGCCTAGCGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTTGGCACCC‑AAGGGTGATCCAAGGACCAAGTCCGGGATGCTTCTTTACGGCAt > 1:487894/1‑239 (MQ=255) tttgggCGACAACGGTTTCCGATTTCGTACGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCAttt < 2:1068396/234‑1 (MQ=255) gTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGCAACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGCTCTGGAAAAAGTGCGTAAAGGGTTACTGACGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGCACCAAAGCCGGAGACCTTCTTTCAGGCCTTTGGt > 2:652544/1‑239 (MQ=255) gTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCCCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTggg > 1:1068396/1‑238 (MQ=255) gTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGGCTCGGCGTGGCCTATGGCTCCGTCCTGGGAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGGGCACCC‑AAGGGGGAGGCCCAAACCAAAGCCGGAAGTCTTCTTTACGGCATTTgg > 2:424761/1‑238 (MQ=255) tGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGGAAAGTGTTACTGAAGGCGGCGACTGGGCACCC‑CAGGGTGATGCACGAACCAATGCCGGAAGTCTTTTTTACGGCATTTggg > 2:753128/1‑237 (MQ=255) cGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑CAGGGGGATGCCGGAACCAAGGCGGAAAGCCTTCTTTCCGGCATTTGGTGcc > 1:65269/1‑239 (MQ=255) aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑CAGGGGGAGGCACGAACAAATGCCGGAAGGCTTTTTTACGGCATTTgtggcaac > 2:456265/1‑232 (MQ=255) aaaCGATTACCGATTTCGATCGGAAAGAAGTGATCATCCCGAACAAAGCG‑GTGGTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGAAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg < 1:629581/237‑1 (MQ=255) ttttccGATTTCGATCGCAAAGAAGTGATCCTCCCGAACAAAGGC‑TTTGTGACCGGGCGTCTGATCCACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt < 2:968809/235‑1 (MQ=255) ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt < 1:208660/238‑1 (MQ=255) ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt < 1:1014053/238‑1 (MQ=255) aCCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGAACCAATGCCGGAAGCCTTCTTTACGGCATTTGGTGCCAGCCCGTTggg > 1:832164/1‑238 (MQ=255) gATCCCAAAGAAGTGTTCCTCCCGAAAAAAGCT‑TTTTTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt < 2:421261/239‑1 (MQ=255) aTCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCTTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGCCGACTGAGCACCC‑AAGGGTGATGCAAGAACCAATACCGGAAGGATTCTTTCCGGCATTTGGGGCCGGCagttttgacactaagcag > 2:706520/1‑222 (MQ=255) tcccAAAGAAGTTGTCATCCCGAACAAAGCT‑TTTGTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGc < 2:178074/236‑1 (MQ=255) agtgtctctcCCG‑ACAAAGGGTTTTGTTGCCTGGGGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGAGCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa < 2:487894/229‑1 (MQ=255) agTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCGGATCAACTGGTCGTTGACTGACGCTACTACGCGTCTGGTGATCCGTCGCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACGC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa < 1:1054756/237‑1 (MQ=255) cccGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCCCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGTGAATGCCCGAACCAATGCCGGAAGTCTTCTTTTCGGCCTTTGGTGCCAGCCAGTTGGACCATTAGCTGCCTCTGTATTTGCGTGAAc > 2:277739/1‑239 (MQ=255) gAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTCCGTCTGTATGTGCGGGAACGGc > 1:671485/1‑239 (MQ=255) aaaaaGCG‑TTTGTTACCGCGCGTTTGATCAACTGGTCGTTGACTGACACTACTACGCGGTTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGt < 1:706520/237‑1 (MQ=255) aaaaaGCG‑GTTGTTACCGAGGGTTGTGTCAACTGGTCGTTGACTGACAGTTCTACGCGTCTGGTGAGCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCTGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACGGCGt < 1:826059/237‑1 (MQ=255) aGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGCGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGCCTTCTTTACGGCATTTGGGGCCAGCACGTTGGCTCATGAGCTGTGTCTGTATGGGCCGGAACTGCGTGccc > 1:36757/1‑239 (MQ=255) gCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATAAGCCGCGTCTGTATGTTCGTGAAATGCGTGACCg > 1:103791/1‑239 (MQ=255) ttGTTACCGAGCGTCTGCTCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGCTCTGGCAAAAGGGCGTAAAGTGTTACTCAAGGCGGCGACTGCGCACCC‑AAgg > 1:212387/1‑137 (MQ=255) aCCGGGCGTCTGACCAACTGGTCGTTGACTGACACTACTACGCGTCGGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc < 1:219839/239‑1 (MQ=255) cGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCga > 1:62623/1‑234 (MQ=255) cGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGt > 1:976821/1‑235 (MQ=255) aGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTGGGATAATGAGCTCGGTCTGTATGTGCGTGAACTGCGGGACCGTAGGCGTAGTgcg > 2:106745/1‑237 (MQ=255) cGTCTGATCAGCTGGTCGGTGACTGACCCTACTGCGCCTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGGTACTGAAGGCGGCGACTGAGCTCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg < 1:832152/238‑1 (MQ=255) ttttCAACTGGGGGTGGACTGACACTACTACGGGGTTGGTGAGCCGTCGCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg < 2:573417/236‑1 (MQ=255) tGATCAACTGGTCGTTGACTGACACTACTACGGGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg < 1:456265/239‑1 (MQ=255) ataaccTGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCt < 2:52222/234‑1 (MQ=255) cacttGGTCGTTGACTGACACTGCTTCGCGGCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCACC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTGTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTCCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑g < 2:162215/234‑1 (MQ=255) aaCTGGTCGTTGACTGACGTTACTACGGGTCTGGTGATCCGTCACGGCGTGGCCTATGGCACCGATCTGGAAAAAGTGCGTAAAGTGTTAATGAAGGCGGCGACTGAGCACCG‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa < 1:507669/239‑1 (MQ=255) aaCTGGTCGGTGACTGACCATACTACGTGTCCGGTGATCCGTCTCGGCGTGGCCTGTGGCTCCGATCGGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa < 1:442352/239‑1 (MQ=255) aaCTGGGCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa < 1:446964/239‑1 (MQ=255) cGTTGACTGACCCTACTACGGGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTAc < 2:874145/238‑1 (MQ=255) cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATCTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGAGACCGTAGTCGGACTGTCGATGAGCT‑GAACCGTACt > 1:63016/1‑239 (MQ=255) cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGTTGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTCCGTCTTTAGTGGCGTGAACTGCGTGACCGTGTTCGTACT‑TCGGTGAGCTGGAACCGAACt > 2:640001/1‑239 (MQ=255) gactgacCCTTCTACGGTTCTGGTGCTCCGTCTGGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCg < 2:671485/239‑1 (MQ=255) gctgacACTCCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTa < 1:102516/231‑1 (MQ=255) actgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATTTTCGTGAACTGCGTGACCGTAGTCGTACTTTCGATGAGCT‑GCACCGGACTATCGa > 1:545388/1‑239 (MQ=255) gactctcctcgggttgggggATCCGTTCCGGCTGGGCCTAGGGCCCCGAATTGGAAAAAGGGCGTAAAATGTTACTGAAGGCGGCGACTGAGCCCCC‑AAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAg < 2:719445/220‑1 (MQ=255) ctacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGGGCCAGCACGTTGGATCATGAGCTGCGTCTTTTTGGGCGTGAACTGCGTGACCGAAGTCGGCCTGTCGGTGAGCT‑GAACCGAACAATAGATAAGAtggc > 2:102516/1‑236 (MQ=255) cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑aa < 2:491509/90‑1 (MQ=255) cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑aa > 1:491509/1‑90 (MQ=255) gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGCAGTCTTCTTTACGGCATTTGGGGCCAGCACGTTGGATCATGAGCTGCGTCTGTCTGTGCGTAAACTGCTGGCCCGTAGACGGACTGTGGAGGGGCT‑GAACCGGCCTATCGATTACCTGGTCCGTg > 2:771480/1‑239 (MQ=255) gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc > 1:378610/1‑198 (MQ=255) gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc < 2:378610/198‑1 (MQ=255) gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa < 2:483454/138‑1 (MQ=255) gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa > 1:483454/1‑138 (MQ=255) ttgtgtttCCGTGTCGGGGGGGCCTTGGGCTCCGATCTGGAAAAAGTGCGTAAAGGTTTTCTGAAGGCGGTGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaaa < 2:569309/232‑1 (MQ=255) cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGCCTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTTGATCATGAGCTGCGTCTGTATGTGCGTGAACTGAGGGACCGTAGGCGTACTGTCGTTTGCTT‑CAACCGCACTATCCTTCAGCtttctgcgaaaaa > 2:686720/1‑227 (MQ=255) cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaaa > 1:498267/1‑239 (MQ=255) cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTTGGATCATGAGCTGCGTCGGTATGTGCGTGAACTGCGGGACCGTAGGCGTACTGTCGATGCGCT‑GAACCGGACTATCGaccctgtctctttaaaaa > 2:137030/1‑221 (MQ=255) cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa < 1:680088/209‑1 (MQ=255) cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGGTCAGCTGTGCCGTGaaaa > 1:146325/1‑239 (MQ=255) cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGGGaaa > 1:569309/1‑238 (MQ=255) atGGTGATCCGGTTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaa < 1:377748/237‑1 (MQ=255) ctgTGATCCGTCTCGGCGGGGCCTATGGCTTCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaaa < 1:931275/236‑1 (MQ=255) ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGTTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGGGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGGGCT‑GGACCGGAATATCGATCAGCTTTGCCGGGAAAAcc > 2:643996/1‑238 (MQ=255) ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg < 2:476255/135‑1 (MQ=255) ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg > 1:476255/1‑135 (MQ=255) ccGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGACCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGCCCGGAGTCGACCTTGCGATGAGCT‑GAACCGAACTATACATCAGCTGGGCCGGGAAAACgcaataa > 2:1031670/1‑233 (MQ=255) ttctgCGGGGCCTTTGGCTCCGGTCTTGAAAAAGTGCTGAAAGTGTTATGGAAGGCGTGGACTTAGCCCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGGCGTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑TAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACAt < 2:608442/235‑1 (MQ=255) ctgggCGGGGGCTTTGGACCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACa < 1:344215/235‑1 (MQ=255) tcGGGGTGGCCTGTGGCGCCGATCGGGAAAAAGGGGGTAAAGGGGTACGGAAGGCGGCGACTGAGCACCA‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTGACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGGGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACAt < 2:875019/238‑1 (MQ=255) cGGCGTGGGCTTAGGCTTCGGTCGGGAAAAAGTGCGTAAAGTTTTAATGAAGGCGGGGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGGCGGAAGGTTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTg < 1:413450/239‑1 (MQ=255) cGATCTGGAAAAAGTGGGTAAAGGGTTAATGAAGGCGGCGACTGAGCGCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAg < 1:640001/239‑1 (MQ=255) cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑ACGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACTTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTCACCGCAGTCGAACTTTCGGTGACCT‑GAACGGTCCTACCGGTAAGCCTTGCCGGGAAAACCACAATCAA‑ACTTCCTTTAACCAGCTTTAAg > 2:644682/1‑239 (MQ=255) aaGGGCGGAAAGTTTTACTGAAGGCGGGGACTGAGCACCC‑AAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGcac < 2:482498/239‑1 (MQ=255) aGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATTAGCT‑GAACCGAACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAAccctgccccttataaacatctcc > 1:653688/1‑218 (MQ=255) gTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTAACAGTAGTCGTACTGTCGATGAGCT‑GCACCGTACTATCGATCAGCTGGGCCGGGAAAACGAC‑ATCAACAAGGCCTTAAACCAACTTGAAGGGctccggaacaagaaa > 2:179439/1‑225 (MQ=255) aaaGTGTTACTGAAGGCGGCGACTGAGCCCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGaa < 1:404661/239‑1 (MQ=255) | TTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAA > NC_000913/489378‑489831 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |