Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 520,534 A→G 24.8% E40G (GAG→GGG)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913520,5340AG24.8% 101.1 / 5.3 56E40G (GAG→GGG) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (8/32);  new base G (13/2);  total (22/34)
Fisher's exact test for biased strand distribution p-value = 9.93e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCT  >  NC_000913/520298‑520771
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              
tgTTTTCCCTCAACCGTGAACATGGCCCCCGGTTGATTATGGTGACCCACGACCTGCAACGGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGgaga                                                                                                                                                                                                                                              <  1:78032/239‑1 (MQ=255)
      ccccCAAACGTGACCATGGCACCCCGTTGATTATGGTGACCCACCGCCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTTCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCt                                                                                                                                                                                                                                        <  1:602222/239‑1 (MQ=255)
         tCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCCCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGGGCTGGCGCTGGGCAATATCAGCGATCGCATGGGGACGGGCttt                                                                                                                                                                                                                                      >  1:659607/1‑237 (MQ=255)
          cAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAAAGGGCAGTTGCAGGAGGAAGCATGATGGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATAAATTTTCTGGCTGGCCCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCAAGCACAAGGGGAAGGGCTAAAg                                                                                                                                                                                                                                    >  2:145554/1‑239 (MQ=255)
                tGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGGTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGGCTGCGTGCTTGGACTGGGGAAATACAGCGATCGCATGGGGAAGGGGTTAAGCCAgac                                                                                                                                                                                                                              >  2:684134/1‑237 (MQ=255)
                 gAACATGGCACCACGTTGATTATGGTTACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCa                                                                                                                                                                                                                              <  1:768049/238‑1 (MQ=255)
                       ggCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTTTCTGGCTGGCGCTAAGCCTGGCGGGGGCCTGCGTGCTGGGGCTGGGCCATATCCGCGATCGCATGGGGAAGGGCTTAAGCCAGCaaaagcgg                                                                                                                                                                                                                       >  2:96699/1‑234 (MQ=255)
                       ggCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGGGCTGGCGCTGGGCAATATCAGGGATCGCATGGCGAAGGGGTTAAGCCCGCAAAGCCGt                                                                                                                                                                                                                       >  1:480769/1‑239 (MQ=255)
                         ccccccGTTGATTATGGTGACCCCCGGCCTGCAACTGGCGGCCCGCTGCGACCGCTGCTTACGGCTGGGGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCg                                                                                                                                                                                                                        <  2:688409/231‑1 (MQ=255)
                              cGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGGGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTa                                                                                                                                                                                                                >  1:894890/1‑239 (MQ=255)
                                 tGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGCACGGGCAGTTGCAGGGGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCCTTCGCCGTCGCTATTAATTTTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGGGCTGGCCGTGGGCGATATCAGCGCTCGCAGGGGGAAGGGGTTAAGCCCGCaaaagccggggtttaagg                                                                                                                                                                                                             >  2:788725/1‑224 (MQ=255)
                                  gATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATggc                                                                                                                                                                                                            <  1:276603/239‑1 (MQ=255)
                                   aTTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGGGCTGGGCAATATCAGCGATCGCATGGGGAAGGGCTTAAGCCAGCCAAGCCGGGAGTTTATg                                                                                                                                                                                                              >  1:688409/1‑236 (MQ=255)
                                   aTTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATTGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATggcg                                                                                                                                                                                                           >  1:851442/1‑239 (MQ=255)
                                   aTTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGc                                                                                                                                                                                                                                   <  1:231610/215‑1 (MQ=255)
                                    ttttggtccccccCGCCCTGCAACGGGCGGGCCCCTGCGGCCGCTGCTGACGGGTGGGGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCGGGCGCGAATGGCTTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATggcg                                                                                                                                                                                                           <  2:737008/229‑1 (MQ=255)
                                       tGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGGGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAACCTGGCGGGGGCCGTCGGTGTTGCCCTGGGCAAAATGCGCGATCGCCTGGGGAAGGGGGTAAGCCCGCAAACACGGGGGTTTagtggggggg                                                                                                                                                                                                       >  2:276603/1‑230 (MQ=255)
                                             cccACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTCCGTGCTGGCGCTGGGCACAATACGCGATTCCATGGGGAAGGGCTTTAGCCACCAAAACCCGGGGTTTTTgggggggggggggg                                                                                                                                                                                                 >  2:911200/1‑231 (MQ=255)
                                             cccACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGGGGGCTCCGTGATGGCGCTGGGCAAAAATAGCGGTCCCAGTGGAAAGGGCTTAACCCAGCAAAACCGTGGGGTTCTggggcgggggcagg                                                                                                                                                                                                 >  2:748768/1‑227 (MQ=255)
                                             cccACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGGGGCCTGCGGGCGGGCGCTGGGCAATATCCGCGATCGCATGGGGAAGGGCTTAAGCCAGCAAAACCGGGGGTTTATggggggggggcggg                                                                                                                                                                                                 >  2:847089/1‑231 (MQ=255)
                                             cccACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGGTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTCAGACTGGCGGTGGCCTGCGTGCTGGCGCTGGGCCATATCAGCGAACGCATGGAGAAGGGCTTAAACCAGCACAACCgccggcccttatacccaactcac                                                                                                                                                                                                  >  2:231610/1‑216 (MQ=255)
                                                     cTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTgcg                                                                                                                                                                                          <  1:781401/238‑1 (MQ=255)
                                                              gcggcACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGGGAAGGGCTTAAGCCAGCAAAGCCGTGGGTTTATggcgggcggcaggggggtggcccgtttccc                                                                                                                                                                                 >  1:453973/1‑216 (MQ=255)
                                                                             cGCTTCTTCCGGGTGGGGAACGGGCAGTTGCAGGAGGAAGTATTATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                                 <  2:1044867/239‑1 (MQ=255)
                                                                             cGCTGTTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACTTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                                 <  2:833376/239‑1 (MQ=255)
                                                                             cGCTGGTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                                 <  1:96699/239‑1 (MQ=255)
                                                                               ctgctTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGGGCTGGCGCTGGGCAATGTCCGCGAGCGCATGGGGAAGGGCTTAAGCCACACAGGCCGGGGGTTGTTGGCGGGCGGTCGGGGGGGGCGCGGCTGACGCGGAGGGGGGCAAggggg                                                                                                                                                               >  2:1078776/1‑235 (MQ=255)
                                                                                 ggtttgggCTGGTGACGGGGCAGGTGCAGGAGGAGGCTTGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGc                                                                                                                                                             <  2:851442/233‑1 (MQ=255)
                                                                                        gCTGGTGAACGGGCAGTTGGAGGAGGAAGCATGAGTGCACGGTGGTTCTGGCGCGAATGGCGGTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTggagg                                                                                                                                                       <  1:374173/238‑1 (MQ=255)
                                                                                                     cAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGGCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAgcg                                                                                                                                          <  1:263997/238‑1 (MQ=255)
                                                                                                      cgTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAgcgc                                                                                                                                         <  1:939930/237‑1 (MQ=255)
                                                                                                                   aGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGGGATCGGGGGGTGGGCAGGTTACGCGGAAGGCCGCCAGCGTGGCTGGGGGGAAGGCACAAACGCGGCCCGaaaagcg                                                                                                                            >  1:214455/1‑234 (MQ=255)
                                                                                                                      attgttGCCCGTTGGTTCGGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCa                                                                                                                         <  2:894890/234‑1 (MQ=255)
                                                                                                                         aTTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGGGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGGGATCGGGGGGGTGGCGGGTCCCCCGAAGGGGCGCAAGCGGGGGTGGGGGAAGGGCAAAAACGCGGGCCGAAAAAAGGCAagag                                                                                                                     >  2:133670/1‑237 (MQ=255)
                                                                                                                           tGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCg                                                                                                                                                                 <  1:705632/193‑1 (MQ=255)
                                                                                                                             cACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACCCGAAGTGCCGCCAGCGTGGCTGGAGGAAGCGCCAAAACGCCGCCCGAAAATCGGCCagcagca                                                                                                                  >  1:571963/1‑237 (MQ=255)
                                                                                                                                         ggCGCATATTGCGTTGGCCGTCTCTATTTATTGTCTGGCTGGCGTTTAGCCTGGGGGTGGCCTGGCTTGTGGCGCTGGGCAATTTCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCCCGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCcaca                                                                                                    <  2:458471/239‑1 (MQ=255)
                                                                                                                                                          ccGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCCGTTCACACGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAACGCGGCCTGAAAGTCGGCAAGCCACTGACTTTTGCCACACAGACCctttaccgcg                                                                                    >  1:254531/1‑231 (MQ=255)
                                                                                                                                                                  tataaggggctggcgtgggcTAAGCGTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCg                                                                            <  1:448791/220‑1 (MQ=255)
                                                                                                                                                                   ttAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGGTCACGCGAAGTGCCGCAAGCGTGGCTGGGGGAAGCGCAAAAACGCGGCCTGAAAGTCGGCAAGCAGCTGGCTTTTGCCCACAAGACCTTTTCAGGGGACACAccccc                                                                          >  1:788139/1‑236 (MQ=255)
                                                                                                                                                                           ctggctggCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCg                                                                   <  1:684134/238‑1 (MQ=255)
                                                                                                                                                                            tggcgggcGCTAAGGCTTGGGGGGGCCTGCGGGCCGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGaa                                                                 <  2:214455/239‑1 (MQ=255)
                                                                                                                                                                                           cTGGCGTTGGCCTGCGTGCTGGCGCTGGGCAATGTCATCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGTCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGga                                                  <  1:119671/239‑1 (MQ=255)
                                                                                                                                                                                            tGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgat                                                 <  1:145554/239‑1 (MQ=255)
                                                                                                                                                                                            gggggggggCCGGCGGGCGGGCGCGGGGAAATAACCGCGCTCGCAGGGGGAGGGGCGTAAGCCAGAAAAGCCGTGAGTTTATGGGGGGCGTTCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgat                                                 <  2:991178/232‑1 (MQ=255)
                                                                                                                                                                                                ggTGGCCTGCGTGCTGGCGCTGGGCAATTTCAGCGAGCGCAGGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGgatgat                                              <  2:76837/238‑1 (MQ=255)
                                                                                                                                                                                                  tGGCCTGGGTGGTGGCGCTGGGCAATATTAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGatat                                            <  1:335079/238‑1 (MQ=255)
                                                                                                                                                                                                  tGGCCTGCGGGGTGGCGCTGGGCAATTTCAGCAGTCGAATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGatat                                            <  1:692058/238‑1 (MQ=255)
                                                                                                                                                                                                      cTGCGTGCTGGCGCTGGGCAATTTCAGCGATCGGATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAc                                        <  2:453973/238‑1 (MQ=255)
                                                                                                                                                                                                       tGCGTGCTGGCGATGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAcc                                       <  1:178464/238‑1 (MQ=255)
                                                                                                                                                                                                       ggCGTGCTGGCGCTGGGCAATAGCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTAcc                                       <  1:302949/237‑1 (MQ=255)
                                                                                                                                                                                                        gggTGCTGGGGCTGGGCAATATCAGCGGTCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCg                                     <  2:788139/237‑1 (MQ=255)
                                                                                                                                                                                                                  gcTGGGCAATGTCAGCGATCGCAGGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGc                            <  1:747847/238‑1 (MQ=255)
                                                                                                                                                                                                                          ataaccgCGGTCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCa                    <  1:976841/232‑1 (MQ=255)
                                                                                                                                                                                                                           tatCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGGGCCGCAAGCGTGGCTGGAGGAAGCGCACAAGCGCGGCCTGCAAGCCGGCCAACAGCGGAGTTTCGCCCCAATGACCCTTTCAGGCGGCACACCGCCGCTGGGGACCGCCAAAACGGGGGGGGGTATCTTCCCCATGGATGGGGAtgcgacaa                  >  2:128659/1‑232 (MQ=255)
                                                                                                                                                                                                                               cggtgtCGCGTGGGGGAGGGCTTAAGCCTGCAAAGACTTGGGTTTATGGCTGGCGGTCGGGCGTTGCGCATTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTaa              <  2:411039/234‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCATGGAGAAGGGCTTAAGCCAGGAAAGCCGGGAGTTTATGGGGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATccccc        <  2:254531/239‑1 (MQ=255)
                                                                                                                                                                                                                                            gagaAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCCCGCGAAGTGCCGCAAACGGGGCTGGGGGAAACGCCAAAACGCGGCCCGAAAGTCGGCAAGCAGCTTGCTTTCGCCCCCAAGACCTTTGCAGGCGACCAACCGCCGCCGGCGACCGCCACAACGGGGGAGGTTAACTACCCGATGAAGGGCGCGCTGccaccaacacacccccgccc  >  2:993010/1‑220 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              
TGTTTTCCCTCAACCGTGAACATGGCACCACGTTGATTATGGTGACCCACGACCTGCAACTGGCGGCACGCTGCGACCGCTGCTTACGGCTGGTGAACGGGCAGTTGCAGGAGGAAGCATGATTGCACGTTGGTTCTGGCGCGAATGGCGTTCGCCGTCGCTATTAATTGTCTGGCTGGCGCTAAGCCTGGCGGTGGCCTGCGTGCTGGCGCTGGGCAATATCAGCGATCGCATGGAGAAGGGCTTAAGCCAGCAAAGCCGTGAGTTTATGGCGGGCGATCGGGCGTTGCGCAGTTCACGCGAAGTGCCGCAAGCGTGGCTGGAGGAAGCGCAAAAGCGCGGCCTGAAAGTCGGCAAGCAGCTGACTTTCGCCACAATGACCTTTGCAGGCGACACACCGCAGCTGGCGAACGTCAAAGCGGTGGATGATATCTACCCGATGTATGGCGATCTGCAAACTAATCCCCCTGGCCT  >  NC_000913/520298‑520771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: