Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 541,102 A→T 14.5% M180L (ATG→TTG)  ybbY → putative uracil/xanthine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913541,1020AT14.5% 114.4 / 3.9 54M180L (ATG→TTG) ybbYputative uracil/xanthine transporter
Reads supporting (aligned to +/- strand):  ref base A (35/10);  new base T (4/4);  total (40/14)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

TCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGTCTGGTAAATATCAGCAATACCTA  >  NC_000913/540890‑541334
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
tctcCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGAGGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGAt                                                                                                                                                                                                                                         <  1:158171/214‑1 (MQ=255)
  tcCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGGCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGGCCCCGCGGGCGGGGGTGTGCCCGGTatcggggaggatatact                                                                                                                                                                                                               >  2:488804/1‑222 (MQ=255)
       cGTGCTGACGATGTTGATTGGTTTTAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGTTGTGCCTGGAACTGGGGTTGTTTATCTTCCTg                                                                                                                                                                                                          >  1:1072995/1‑238 (MQ=255)
            tGACGATGTTGATTGGTTTTAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCa                                                                                                                                                                                                     >  1:17854/1‑238 (MQ=255)
              aCGATGTTGATTGGTTTTAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCTGCCTCTCGGGGGCGGGGTGGTGCCTGGGTCTGGGCATGATTATCTTCCTCCCGCAACg                                                                                                                                                                                                  >  2:551813/1‑239 (MQ=255)
                              ttAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGGGATTTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCCACCTTTTTCCCGtttgggc                                                                                                                                                                                  >  2:198878/1‑234 (MQ=255)
                                     aTTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGGGATGTGCCTGGTACTTGCGGTGATTATCTTCCTGCCGCCACGTTTTGCCCGTTATGGCCGGCTgg                                                                                                                                                                           >  2:868530/1‑239 (MQ=255)
                                      ttAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGTTGTGCCTGGTACTGGCGATGGTTATCTTCCTGCCCCCACGTTTTGCCCGTTAAGGCCCGCTGGt                                                                                                                                                                          >  2:840131/1‑239 (MQ=255)
                                               cGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGTCGCTGCTTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCCGCTGGTCGGCTCCAt                                                                                                                                                                 >  1:397360/1‑239 (MQ=255)
                                                           ttATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCCCCCTAACCggttggtt                                                                                                                                                     >  1:1084296/1‑239 (MQ=255)
                                                                   gCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGttgttggtgg                                                                                                                                             >  1:127180/1‑235 (MQ=255)
                                                                   gCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTgg                                                                                                                                              >  1:896022/1‑238 (MQ=255)
                                                                   gCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGGGGCGGCGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGGTATGGCCGGCTGGTCGGCCCCCTAACCGGCTGGTGGTTGTGGt                                                                                                                                             >  2:346809/1‑239 (MQ=255)
                                                                   gCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGGGCCGGTGATGTGCCTGGTACTGGGGCTGAATATCTTCCTTGCGCAACGGTTTGCCCGTTATTGCCTCCTGGTCGGGACCCAAAACGCCGGGttgttggtgg                                                                                                                                             >  2:1018450/1‑235 (MQ=255)
                                                                     cGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGTTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGGGAGGGGCCTGGTCCTGGCGGTGATTTTCTTCCTGCCGCACGGTTTTGTCCGTTATGGCCTCTTGGTGGGCCCCCTAACCgccggggcggttgggact                                                                                                                                           >  2:1013575/1‑222 (MQ=255)
                                                                     cGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCATTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGAGCCTTGTACTGGCGCTGATTAGCTTCCTGCGGCAACGTTTTGCCCGTTATGTCCTGCTGGTCGGCACCATAACCGGCTGCTTGTTGTGGt                                                                                                                                             >  1:1029973/1‑237 (MQ=255)
                                                                     cGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGGCTGCTGGTAGGGACCATAACCGGCTGGTTGTTGTGGt                                                                                                                                             >  1:275299/1‑237 (MQ=255)
                                                                     cGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTACTTGGTAATGGCGATGATTATCTTCCTGCCGCAACCTTTTGCCCGTTATGGCCCGCTGGCCGGCACCCTAACCGGGTGgttgtttgggt                                                                                                                                             >  2:83946/1‑232 (MQ=255)
                                                                     cGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGCGTTGTGCCTGGTACTGGCCATGATTACCTTCCTGCCGCCACGGTTTGCCCGTTATGTCCTGCTGGTCGGCACCATAACCCGCTGGTTGTTTTggg                                                                                                                                             >  2:808499/1‑236 (MQ=255)
                                                                       tCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGGGGTACtt                                                                                                                                         >  1:301946/1‑239 (MQ=255)
                                                                                           ttgtgcgcgggggccgcAGCCGGACACTTTCTTTTTTAAAGGGTAGGCTGGGGTTGCGGTTGGGCATGGCCGCCCGAATTTTAAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCAc                                                                                                                      <  1:551813/225‑1 (MQ=255)
                                                                                           gTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCggca                                                                                                                      >  1:351448/1‑235 (MQ=255)
                                                                                           gTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCAc                                                                                                                      >  1:791179/1‑238 (MQ=255)
                                                                                           gTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCCCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCAc                                                                                                                      >  1:784595/1‑238 (MQ=255)
                                                                                               aTGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGc                                                                                                                  >  1:59975/1‑238 (MQ=255)
                                                                                               aTGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGc                                                                                                                  >  1:112741/1‑238 (MQ=255)
                                                                                               aTGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGGGGTACTTTTGCTTTCCTTCTTCGCACTCGc                                                                                                                  >  1:1000667/1‑238 (MQ=255)
                                                                                                tgtggggCGCGCAGTTGACCATTATCTTTTTCAAGGGTATGCTGGGGCTGCCGTTTGGCATAGCCGCCCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGct                                                                                                                 <  1:868530/234‑1 (MQ=255)
                                                                                                tGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTCCTTTTGCTTTCCTTCTTCGCACCCGct                                                                                                                 >  1:636138/1‑238 (MQ=255)
                                                                                                    gggCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCg                                                                                                            >  1:101651/1‑239 (MQ=255)
                                                                                                     ggCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCCACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTGCCACTCGCTCTCCgg                                                                                                           >  1:11696/1‑239 (MQ=255)
                                                                                                      gcgcgcAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGACGATTATCTTCCTCCTGCAACGTTTTGCCCGGTTGGGGCTGCGGGTTGGCACCCTAACCGGGTGGTTGTTGTGGGGCTTTGGTTTTCCTTCTCTCACTCGcgccgccggg                                                                                                         >  2:272589/1‑230 (MQ=255)
                                                                                                         cgcAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAAGGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAg                                                                                                       >  1:361713/1‑239 (MQ=255)
                                                                                                                tgcccattattttttCAAAGGGTATGGTCGGGCTGCGGTTTGGCATGGCCGACCAGATTTTTAAATTTCGGTTCCCGCCGTTCGCGCTCTCGGTGGCGGTGTTGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCa                                                                                                  <  1:840131/228‑1 (MQ=255)
                                                                                                                      tATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTTTGGGACTTTTGCTTTCCTTCTTGGCACCCCCCCTCCGGGGGGTTGAACgggaggg                                                                                          >  2:401240/1‑235 (MQ=255)
                                                                                                                            tttCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCCATCGCTCTCCGGTGAGTTGCCCTGGCAGTGGTTc                                                                                     >  1:680580/1‑238 (MQ=255)
                                                                                                                                aaaGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTTTGGCCTGCTGGTCGGCACCATACCCGGCCGGTTGTTGTGGTACTTTTGCTTTTCTTCTTCCCACTCGCTCTCCGGTGAGTGGCACTGGCAGGGGGTCCCGcc                                                                                >  2:513455/1‑238 (MQ=255)
                                                                                                                                        tGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGCACTTTTGCTTTCCTTTTTCGCCCTCGCCCTCCGGTGGGTTGCACTGGCAGGGGTTCCCGCCCGGCAgg                                                                         >  2:570180/1‑238 (MQ=255)
                                                                                                                                        tGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGGGGCGGTGATGTGCCTGGTACTGTCGATGGTTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTGTTTTTGGGACTTTTTCTTTGCTTCTTTCCCATCCCTCCCCGGGGAGTTGGCCTGGcgtgggtgccggcctgtccgg                                                                         >  2:499433/1‑219 (MQ=255)
                                                                                                                                               gCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCCGTGGCgggg                                                                  >  1:176633/1‑237 (MQ=255)
                                                                                                                                                          gCATAGCCGACCTAATTTTTAAAATGTGGTTACCGCCGTTCGCGCTCTCGGTGGGGGGGATGGGCCTGGTACTGGGGGTGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGcc                                                        <  1:957167/238‑1 (MQ=255)
                                                                                                                                                                ccGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACAATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCCCTCTCCGGCGAGTGGCTCTGGCAGGGGTTCCCGCTCGGCAGTGGCggggggtttgtggcccgtgct                                                 >  2:637765/1‑220 (MQ=255)
                                                                                                                                                                   aCCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGcc                                                                                                                                                                                                                                    >  1:976385/1‑57 (MQ=255)
                                                                                                                                                                   aCCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGcc                                                                                                                                                                                                                                    <  2:976385/57‑1 (MQ=255)
                                                                                                                                                                    cccGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTg                                                                                                                                                        >  1:787365/1‑132 (MQ=255)
                                                                                                                                                                     ccGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCCTAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCACTCCGGTGGGTTGCCCTGGCAGGGGTTCCCGCTCGGCGCGGGCGGGGGTTTTTTGCCgggggttttttt                                            >  2:887316/1‑230 (MQ=255)
                                                                                                                                                                             taaaaaTTCGGTTCCCGCCGTTTCCGCTTTCGGTGGCGGGGTTGTGCCTGGTACGGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGAcggcg                                     <  1:1013575/237‑1 (MQ=255)
                                                                                                                                                                               aaaaTTTGGTTCCCGCCGGTCCCGTCTCCGGTGGCGGTGTTGTGCCTGGTTCTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGt                                   <  1:401240/239‑1 (MQ=255)
                                                                                                                                                                                         tcccggcgTTCGCTCTCGCGGGGGCGGTGTTGTGGCGGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGt                         <  1:272589/237‑1 (MQ=255)
                                                                                                                                                                                                    gcgcTTTCGGTGGCGGTGATGTGCCTGGTACGGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGTCTGGTAAatat              <  1:778443/239‑1 (MQ=255)
                                                                                                                                                                                                    gcgcTCTGGGTGGCGGTGATGGTCCTGGTCTTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGTCTGGTAAatat              <  1:942646/239‑1 (MQ=255)
                                                                                                                                                                                                    cggcTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGTCTGGTAAatat              <  1:586784/237‑1 (MQ=255)
                                                                                                                                                                                                           ccGGTGGGGGGAATGGTCCTGGTACTGGCGTTGATTACCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGTCTGGTAAATATCAGCaa        <  1:808499/238‑1 (MQ=255)
                                                                                                                                                                                                                  cGGTGATGGTCCTGGTGCGGGCGGTGATTCTCTTCCTGCCGCAGCGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGGTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGTCTGGTAAATATCAGCAATACCTa  <  1:488804/238‑1 (MQ=255)
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
TCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGATTAGGCCATCGCCTGGCACGGTTATTTACGCCGTCGGTGATGGTCTTGTTTATGTTGATGCTGGGCGCGCAGCTGACCACTATCTTTTTCAAAGGTATGCTCGGGCTGCCGTTTGGCATAGCCGACCCGAATTTTAAAATTCAGTTACCGCCGTTCGCGCTCTCGGTGGCGGTGATGTGCCTGGTACTGGCGATGATTATCTTCCTGCCGCAACGTTTTGCCCGTTATGGCCTGCTGGTCGGCACCATAACCGGCTGGTTGTTGTGGTACTTTTGCTTTCCTTCTTCGCACTCGCTCTCCGGTGAGTTGCACTGGCAGTGGTTCCCGCTCGGCAGTGGCGGTGCTTTGTCGCCGGGAATTATTCTGACGGCGGTGATTACAGGTCTGGTAAATATCAGCAATACCTA  >  NC_000913/540890‑541334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: