Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,158,048 A→C 24.8% P60P (CCA→CCC ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,158,0480AC24.8% 98.0 / 4.7 73P60P (CCA→CCCptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (19/32);  new base C (15/5);  total (35/38)
Fisher's exact test for biased strand distribution p-value = 7.44e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAA  >  NC_000913/1157816‑1158284
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
tGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGGAAACATGCCCCTGGtt                                                                                                                                                                                                                                             >  1:628599/1‑239 (MQ=255)
  ccACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGCCTTTGTAAACATGGCACTGAtttt                                                                                                                                                                                                                                           >  1:237197/1‑239 (MQ=255)
       cgTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTTCGCCGTTGCAAACATGCCCCTGtttttttgga                                                                                                                                                                                                                                      >  2:890663/1‑235 (MQ=255)
              aCGTAAAAAAACCCCCAATACCAAGGAGCACTCTCAATTATGTTTAAGATTGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGgtgt                                                                                                                                                                                                                               <  1:933759/239‑1 (MQ=255)
                       aaGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATTGCAGAAGCAGGCGGTTCCGCCTTTTCCAAAAATCCCACTATTTTTTgtaacgggggggcccccgg                                                                                                                                                                                                                       >  2:497926/1‑221 (MQ=255)
                            cccATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCCCTGATTTTTTCGATCCGGGTTGCCCTCGGCttt                                                                                                                                                                                                                 >  1:727630/1‑238 (MQ=255)
                                 aagttttgttaaaggaacgggtGTTTAAGAATGTGTTTGCTACCCAGAAAAGGGTGGGAAAATCGTGGTTGCGGCGGGTTCCCTTCGTGCCTTCGGGGGGTATTTGGGGGGGGGTCGGTTCCGGAAATTTCGGGTGGCGGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCaa                                                                                                                                                                                                            <  2:19811/217‑1 (MQ=255)
                                       gAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTTCGCCTTTTCAAACAAGCTCACGGTTTTTTGCGCCGGGTGTCCCCCCGGGCTTTGCAAAAAAcat                                                                                                                                                                                                      >  2:613376/1‑237 (MQ=255)
                                       gAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGGCTTTTCAAACATGCCACCGATTTTTTCGATCGGTGTCGCCCCCGGCTTTTCCACTAACAAt                                                                                                                                                                                                     >  1:808518/1‑239 (MQ=255)
                                          cACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAACTCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCCCTGATTTTTGTGATCGGGTTCCGCCCCGGCGTTACCAATaaagcgtgg                                                                                                                                                                                                  >  1:855484/1‑232 (MQ=255)
                                          cACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAAAATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCACTAACCATGGc                                                                                                                                                                                                  >  1:297269/1‑239 (MQ=255)
                                          cACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGCCCTTTCAAACATGCCCCCTGTTTTTTGAGCTGGGTGTGCCCCCTGCCTTTCCAAAAACCAAGGc                                                                                                                                                                                                  >  2:699957/1‑239 (MQ=255)
                                          cACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCCTCTTTGCTACCATTCCACTCATTTTTTAGATTGGGGTCGCCCCCGCCTTTTCCAAAAAACATgg                                                                                                                                                                                                   >  1:40964/1‑238 (MQ=255)
                                            ctctCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGCTGTATCGCATGTTATGGCAGAAGCAGGCGGGTCCGCCCTTTCGACCATCGCCCTGCTTTTTGCCATCGGGGGCTCCCCCCGCCTTTCCCAAAAAGATGGCgg                                                                                                                                                                                                >  2:908427/1‑238 (MQ=255)
                                             tctcAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGGTTCGGCTTTTTCAAAAAGGCCCTGATTTTTTCGGACGGGGTGGCCCTCGGCTTTTCCAaaaaaagaggggga                                                                                                                                                                                               >  2:1054994/1‑229 (MQ=255)
                                                   tttttttttacattggtttttCTAACCTAGAAAAGGGAGTAAACGCGCTGCGGCTGCCGTTTCCCGACCCGCCTATCGCGGTTTTTCTTCGGCGTGTCGGTCCCGAGAATTTCGGGTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCcgcg                                                                                                                                                                                         <  2:1054522/222‑1 (MQ=255)
                                                   ttttttttaagattctttttGCTAACCTCAAAAAGGGCGTAAAAGCCTGGTTGCCGCCGGTTCCTGTCCTGCCTATCGCTGGTATTCTGCGGGGCGTGGGTTCCGGGAATTCCGGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCcgcg                                                                                                                                                                                         <  2:261652/222‑1 (MQ=255)
                                                     aTGTTTAAGAATGCATTGGCTACCCTGCAAAAGGTCGGTAAATCGGTGATGCTGCCGGTATCCGTACTGCATACCGAAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCt                                                                                                                                                                                       <  1:66245/239‑1 (MQ=255)
                                                         ttAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGGCCTTGCAAACAGGCCCATTCTTTTTTGCTCCGGGGTCGCCCTCGCGTTTACCAATAACAAATGCGTAAccccccgggg                                                                                                                                                                                    >  2:1060390/1‑230 (MQ=255)
                                                         ttAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCCGCTTTTCAAACATGCCCATGATTTTTTCGAATGGGGGTGCCCCCGGCTTTTCCCAAAACCATGGGGTAACCGCGCGGGcc                                                                                                                                                                                   >  2:624157/1‑239 (MQ=255)
                                                         ttAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCCGCCTTTCAAACCAGCCACTGATTTTTTCGGCCGGTGTGTCCCCCCGCTTTTACCAAAACCAAGGGGGATCCGCGCTGGcc                                                                                                                                                                                   >  2:951915/1‑239 (MQ=255)
                                                         ttAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGGTCCGTCTTTGGCAACATGGCACTAATTTTTGTCATCGGGGGCGCCCTCGGCTTTACCCATAACAATGGGGGATCCGCGCGGGcc                                                                                                                                                                                   >  2:668476/1‑239 (MQ=255)
                                                          tAAGATTGCATTTGCTACCCTGCAAAAGGTCGGTAAATCGCTGAGTCTGCGGGTATCCGTACTGCCTATCGAAGTTATTCTGGTGGGAGTCGGTTCCGGGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCg                                                                                                                                                                                  <  1:890663/239‑1 (MQ=255)
                                                           aaGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCCGCTTTGCCAACATTCCACTGGTTTTTGTGGTTGCTGTCGGCCTCGCGTTTTCCCATAACGTTgcggtactccccctcgccg                                                                                                                                                                                  >  1:1054672/1‑220 (MQ=255)
                                                             aaaTGCATTTGCTAACCTGCAAAAGGTCGGAAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTGCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAg                                                                                                                                                                               <  1:804726/238‑1 (MQ=255)
                                                                tGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCCCTGGTTTTTGCGATCGGGGTTGCCCCCGGCCTTACCAATAACCATggggctgtcccttttacacatctc                                                                                                                                                                             >  1:19811/1‑216 (MQ=255)
                                                                  cATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGGCTTTGCAAACATGCCCCTGAGTTTTGCGATCGGTGGTCCCCTCGGCTTTACCAAAAAACATGGGGTTTTCGCCCCGGCCGCCgttgtt                                                                                                                                                                          >  1:670235/1‑239 (MQ=255)
                                                                  cATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCCGCTTTGCCAAAATGCCCCTGTATTTTGCGGTCGGGGTTGCCCTCGGCGTTTCCAATAACGATGGCGTACCCGCCCTGGCCCCCgctgtt                                                                                                                                                                          >  1:492844/1‑239 (MQ=255)
                                                                       gttAACCTGAAAAGGTTCGGTAAAGCGCTGATGCGCCCGGTACCCGTCCTGCCTATCGGAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTa                                                                                                                                                                     <  1:624157/237‑1 (MQ=255)
                                                                        cTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACAAGCCCCTGATTTTTGCGATCGGGGTCGCCCTCGGGTTTACCAATAACGATGGCCGATCCCCCCTGGCCGCAGTTGTTTcc                                                                                                                                                                       >  1:523356/1‑236 (MQ=255)
                                                                              tgaaaagGGTCGGTAAATGGGTGAGTCTGCCGGTGCCCGTTCTGCCTGTGGCAGGTTTTCTGGGGGGCGTCGGTTCCGGGAATTTCAGCTGGCGTCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGcatc                                                                                                                                                              <  1:951915/236‑1 (MQ=255)
                                                                              gccaaggtggtggTAAATCGCGGGTGCGCGCGGTATCTGTACTCCCTATCGGTGGTATTGTGGTGGGTTTCGGTGCGGGGAATTCCGGTTGGCTGCCCGACGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGcatc                                                                                                                                                              <  2:310699/228‑1 (MQ=255)
                                                                                       ttggtaaaccgtttaggtggcCGGTTTCCGTACTGCCTATCGCAGGTTTTTCGCTGGGGTTCGGTTCCGCAAATTTCAGCTGGCTTCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTaa                                                                                                                                                      <  1:893838/219‑1 (MQ=255)
                                                                                         ggTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTTCAAACATGCCCCTGATTTTTTCGATCGGTGTCGCCCCCGGCTTTTCCAATAACGCAGGCGTATCCCCGCGTGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAAc                                                                                                                                                   >  1:367679/1‑239 (MQ=255)
                                                                                               tccttgtgtctgcCGGTTTCCGTTCTGCCTTCCGCGGGTTTTTTGGGGGGGGTCGTTCCCGGGATTTTCAGCTGGCGCCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGc                                                                                                                                             <  2:911480/230‑1 (MQ=255)
                                                                                                 gttGATGGTGCCGTTACCCGTCCTGCCTATCGAAGGTTTTCTGCTGGGCGTTGGTTCCGCAAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGc                                                                                                                                             <  1:946220/235‑1 (MQ=255)
                                                                                                                  ccGTTCCGCCTACCGCGGGTTTTGTGCTGGGGGTCGGTACCGCGAATTTCAGCTGGTTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTa                                                                                                                        <  1:758121/239‑1 (MQ=255)
                                                                                                                   cgttcgcgcctttgCAGGTTTTCTGGTGGGGCGTCGGTTCCGGAAATTTCAGTTGGCTCCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTa                                                                                                                        <  1:55316/226‑1 (MQ=255)
                                                                                                                            tATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTTCCAAAACGCCACCTATTTTTGCGACCGGTGGCGCCCTCGGCTTTTCCAATAAAGATTGCGCATCCCCCCTTGCCCCCCGTGTTTCCCATTGCACCCTGGTTAAAACCCTGGCCGCGGGTGCCGCCCCCGTATTGCAtttt                                                                                                               >  2:74207/1‑237 (MQ=255)
                                                                                                                              tcccAGGTTGTCTGGGGGGCGTGGGTTCCGGCAATTTCATCGGGATGCCCGCCTTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACct                                                                                                            <  1:917888/236‑1 (MQ=255)
                                                                                                                               cGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGGGTCGCCCTCGGCTTTACCAAAAACGATGGCCTATCCCCGCGTGCCGCAGTTGTTTCCTCTGGCACAACGGTTAAAACCAATGCCGGGGTTGCGCCCCCGGGACTCCAATTAAct                                                                                                            >  2:837694/1‑238 (MQ=255)
                                                                                                                                 tcagtttttcgcggtgtagccGGTTCCGGTAATTACAGGTGGCGGCCCGCCGTTGTATCGCAGGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACctg                                                                                                           <  1:246407/218‑1 (MQ=255)
                                                                                                                                       ttCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTTCGCCCTTCTAAACATGCCACCGCCTTTTTCGCTCGGTGTTGCCCTCGGCTTTTCCAATAAAGATGGCCTTTCCGCGCTGGCCCCGGTTGTTGCCCATTGCATCATGGTTAAAACCCTTGCCGGGGTTGGCGCCCTGGCACTCCCTTTAACCGGTgaaaaa                                                                                                 >  2:969512/1‑239 (MQ=255)
                                                                                                                                           gctgGGCGTCGGTTCGGCGGTTTTCAGGTGGCTGCCCGCCGTTTTAACGCATGTTATGGCAGAAGCAGGGGGTTCCGGCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCg                                                                                             <  1:699957/239‑1 (MQ=255)
                                                                                                                                             cgggggctgtggtCCGCGAATTTTCAGCTGGCTGCCCGCCTTTGTTTCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCCCTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCg                                                                                             <  1:1054994/227‑1 (MQ=255)
                                                                                                                                                ggCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGCAGCAGGAGGTTCCATCCTTGCAAAACAGCAACAGGTTTTTGCGGTCGGTGACGCGCGCGGCTTTACCACTAACGATGGCGTCTCCGCGCTGGCCCGAGTTAGTGTCCATGAGCTCCTGGGTAAAAACATGGAAGTGGATTAGACACTCGTACTGAGTTTACGCTCTGAAAAAAtacccgt                                                                                         >  2:809471/1‑233 (MQ=255)
                                                                                                                                                ggCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCCCGCGTGCCGCCGGTGTTGCCCTTGGCATCCAGGTTTAAACCAAGGCCCTGGTTGCGCCGCCGGTCACTGCTTTAAccgcttaaaaaaaaccccc                                                                                            >  2:392622/1‑222 (MQ=255)
                                                                                                                                                ggCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGCCCTTTCCAACAAGCCCCTGATTTTTGCGAGCCGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTCGCCGCAGTTGTTGCCTATGGCCTCATGGTTAAAACCACGGCCGGGGGTGCGCCCCCGGTACCGCATTTACACGCTGaaaaaaaacacc                                                                                          >  2:449906/1‑232 (MQ=255)
                                                                                                                                                        ttCCGCGCATTTCAGGTGGCTGCCCGCCTTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTTGTTGCAAAGATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAacac                                                                                  <  1:502478/238‑1 (MQ=255)
                                                                                                                                                        tcccGCAAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAacac                                                                                  <  1:635462/236‑1 (MQ=255)
                                                                                                                                                                      gggtggctgCCCCGCGTTTTTTCGCATGTTTGGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCCCTGATTTTTGCGATCGGGGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTgg                                                                    <  2:523356/236‑1 (MQ=255)
                                                                                                                                                                          ggctcCCCGCCTTTTTAGCCCCTGTTTGGGCAGAAGCGGGCGGTTCCGTCTTTGCAAACATGCCCCTGATTTTTGCGAGCGGGGTCCGCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTa                                                                <  2:628599/239‑1 (MQ=255)
                                                                                                                                                                          gcgtcccagccgTTGTGTCGCATTTTATGGCAGAAGCTGGCGGTCCCTTGTTTGCAAACATGCCTGTGAATTTTGCGATCGGTTTCGGCCTCGTCTTTACCAATAACGAGTGCGTCTCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGACGCTAAACACCGGGCGGATACTGGCGTa                                                                <  2:6696/231‑1 (MQ=255)
                                                                                                                                                                                ccgccgTTGTATCGCATGTTAGGGCAGAAGCAGGGGGTTCCGTTTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGGTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGa                                                          <  1:497926/239‑1 (MQ=255)
                                                                                                                                                                                ccctcgTTGTAGCGCGTGTAATGGCAGAAGCGGGCGGTTCCGTCTTGGCAAACATGCCACGGATTTTTGCGATCGGTGTCGCCCTCGGCGTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGa                                                          <  1:908427/235‑1 (MQ=255)
                                                                                                                                                                                cccgtgttgttttccttttttgggCAGAAGCGGGGGTGTCCGTCTTTGAAAACATGCCACGGATTTTGGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGa                                                          <  2:492844/217‑1 (MQ=255)
                                                                                                                                                                                     tttttttCGCTTGTTATGGCAGAAGCGGGCGGTTCCGTCTTTGCAACCATGCCACTGATTTTTGCGGTCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGAt                                                     <  2:959321/233‑1 (MQ=255)
                                                                                                                                                                                            cGCATGTTGTGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTcc                                              <  1:1060390/239‑1 (MQ=255)
                                                                                                                                                                                              cATGTTATGGCAGAAGCAGGCGGTTTCGTCTTTGCAAACATGCCACTGATTTTTTCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCCCGCTGGCCg                                                                                                                                                                                  >  2:671172/1‑109 (MQ=255)
                                                                                                                                                                                              cATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCg                                                                                                                                                                                  <  1:671172/109‑1 (MQ=255)
                                                                                                                                                                                               aTGTTTGGGCAGAAGCAGGCGGTTCCGTCTTTGCGAAAATGCCACTGATTGTGGCGATCGGTTTCGCCCGCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGTAGTTGTGGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACCCCTGGCGGATACTGGCGTACTCGGAGGGATTATCGCCGGt                                           <  2:627584/239‑1 (MQ=255)
                                                                                                                                                                                                   tATGGCAGAAGCAGGCGGTTCCGTCTTTTCAAACATGCCACTGATTTTTTCGATCGGGGTCGCCCCCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGGGGTTTCGCCACCGGTACTGCATTTACCTCCTGAAAGAAATGCCCCTTAACACCCGGCGGGTACTTGGGTACTCCGAGGGAGTAttttccgcgcgg                                       >  1:542456/1‑228 (MQ=255)
                                                                                                                                                                                                    aTGGCAGAAGGAGGGTGTTCCGTTTTTGCAAACATGCCACTGATTTTGGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGAt                                      <  2:42957/239‑1 (MQ=255)
                                                                                                                                                                                                     tGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAAAGATGGCGTATCCGCGCTTGCCGCAGTTGTTGCCTATGGCATCATGGGTAAAACCATGGCCGTGGTTTCGCCCCTGGTACTGCATTTACCTGCTGAAGAAATCCCCTCTAAAAACCTGGCGGTAACTTGGGTACTCCGAGGGATTATTTCCGGGGCGAt                                      >  1:262212/1‑238 (MQ=255)
                                                                                                                                                                                                         aGAAGCAGGCGGTTCCGTCTTTGCAAACGTGCCACTGATTTTTGCGATCGGTGTCGCCCTGGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCa                                  <  1:74207/238‑1 (MQ=255)
                                                                                                                                                                                                           aaGCAGGCGGTTCCGTCTTTGCAAACATGCCCCTGATTTTTTCGATCGGGGTCGCCCTCGGCTTTACCAATAAAGATTGCGGATCCGCGCTGGCCGCAGTTGTTGCCCATGGCATCATGGGTTAAAACATGGCCGGGGGTTGGCCCCTGGGTATGCGTTTTCCTGCCGAAGAAAACGCCCCTTAACACCTGGGGGGTACTGGCGTGCTCGGGGGGGGTATCCTCCGTGGGATCGccggg                               >  2:234605/1‑235 (MQ=255)
                                                                                                                                                                                                             gctggggggTTCCTTTTTTGCAAACATGCCCCTGGTTTTTGCGATCGGTTTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGt                              <  2:262212/232‑1 (MQ=255)
                                                                                                                                                                                                               gggCGGTTCCGTCTTCTCAAACTTGCCACTGATTTTTGCGATCGGTGCCGCCCTCGGCTTTACCAAGAACGATGGCTTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTAc                            <  2:237197/237‑1 (MQ=255)
                                                                                                                                                                                                                 gCGGTTTCGTCTTTGCAAACATGCCCCTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTTTCCGCGCTGGCCGCCGTTGTTGCCTATGGCATCATGGTTAAAACCCAGGCCGGGGGTGCGCCCCTGGGACTGCCTTTTCCTGCCTGAAAAATCGCCTCTCAAAACCCGGCGGGTACTGGGGGACCTCGAGGGATTAATTCCgcggggacacccgcgggcaag                         >  2:182836/1‑219 (MQ=255)
                                                                                                                                                                                                                       tCCGTCTTTGAAAAAATGCCCATGATTTTTGCGATCGGTGTCGCCCTCGGTTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTa                     <  1:670566/238‑1 (MQ=255)
                                                                                                                                                                                                                            tttttGCAAACCTGCCACTGGTTTTTGGCTTCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTTCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGt                <  1:532776/237‑1 (MQ=255)
                                                                                                                                                                                                                                 ggaaaCATGACAGTGATTTTTGCGGTCGGTGTCGCCCTAGGCTTGACCAATAACGATGGCGTAGCCGCGCTGGCCGCAGTTGGTGCCTATGGCATCATGGTGAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTGACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTa           <  1:868029/237‑1 (MQ=255)
                                                                                                                                                                                                                                           ccACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGAGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATAGCACCGTACAGGTTTAACCGGTTCTACCGTATTaa  >  1:1046108/1‑239 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 
TGCCACGCGTGAGAACGTAAAAAAAGCACCCATACTCAGGAGCACTCTCAATTATGTTTAAGAATGCATTTGCTAACCTGCAAAAGGTCGGTAAATCGCTGATGCTGCCGGTATCCGTACTGCCTATCGCAGGTATTCTGCTGGGCGTCGGTTCCGCGAATTTCAGCTGGCTGCCCGCCGTTGTATCGCATGTTATGGCAGAAGCAGGCGGTTCCGTCTTTGCAAACATGCCACTGATTTTTGCGATCGGTGTCGCCCTCGGCTTTACCAATAACGATGGCGTATCCGCGCTGGCCGCAGTTGTTGCCTATGGCATCATGGTTAAAACCATGGCCGTGGTTGCGCCACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAA  >  NC_000913/1157816‑1158284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: