Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,455,879 T→C 17.3% L239P (CTC→CCC)  paaC → ring 1,2‑phenylacetyl‑CoA epoxidase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,455,8790TC17.3% 174.8 / 2.7 77L239P (CTC→CCC) paaCring 1,2‑phenylacetyl‑CoA epoxidase subunit
Reads supporting (aligned to +/- strand):  ref base T (36/24);  new base C (4/11);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 4.04e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACA  >  NC_000913/1455655‑1456115
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
ttttCCCGCCGAACTTTTCGATGCCGACGGGATTGATATTGCCCTGGGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGc                                                                                                                                                                                                                                     <  2:850589/239‑1 (MQ=255)
     ccgccgAACCTGTCGAGTGCGACGAAGATGATAATGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGt                                                                                                                                                                                                                                <  1:445935/239‑1 (MQ=255)
         cgAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCAGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGc                                                                                                                                                                                                                           <  1:1002925/239‑1 (MQ=255)
          gAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGAATCGCACTGGCGGGAAAAAAGGACTGCAAACCGACAACCCTGGACCCAATCTGGGAGAAAGAAAGTATCCTCAGCTGGTACTGCCCCGTCacaa                                                                                                                                                                                                                           >  2:636483/1‑236 (MQ=255)
             cTGTTCGATGCCGACGAGATTGAAATTGCACTGAGTGAAGAGGTTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAAt                                                                                                                                                                                                                       <  1:352904/238‑1 (MQ=255)
                gtCGATGGCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGAACCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTa                                                                                                                                                                                                                   <  1:284367/238‑1 (MQ=255)
                 tCGATGCCGACGAGATTGATATTGCAATGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTa                                                                                                                                                                                                                   <  1:305938/238‑1 (MQ=255)
                   gATGCCGAGGAGGTTGATTTTGCCCTGAGGGAAGGGGGTATTGGGGTTGATCCCCGCACTTTACGCGCAGCGGGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACa                                                                                                                                                                                                                <  2:462508/239‑1 (MQ=255)
                     tGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTTGACCCATGCTGGCAGGAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACaag                                                                                                                                                                                                              >  1:900653/1‑237 (MQ=255)
                      gCCGACGAGATTGATTTTGCACTGAGTGAAGAGGGTTGTGCGGTTGATCCACGCCCTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACagag                                                                                                                                                                                                             <  2:389242/239‑1 (MQ=255)
                           cgaGATTGATATTGCAATGAGGGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGGGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGgaga                                                                                                                                                                                                         <  1:536650/238‑1 (MQ=255)
                           cgaAATTGAAATTGCACTGAGTGAAGAGGGTATTGCGGTTTATCCCCGCAGTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCTCATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAGGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGgaga                                                                                                                                                                                                         <  1:256904/238‑1 (MQ=255)
                               aTTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGAACTGCATACCGACCACCTTTGACCACATCTGGCGGAAATGCAGTAACTCCAGCGTGTCTTTCCCGGTCAACacagggaaaaaagggagggggg                                                                                                                                                                                                     >  2:840492/1‑218 (MQ=255)
                                   atatTGCACTGAGGGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGc                                                                                                                                                                                                <  1:880047/239‑1 (MQ=255)
                                    tatTGCACTGGGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCa                                                                                                                                                                                               <  1:926910/239‑1 (MQ=255)
                                    tatTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGgaga                                                                                                                                                                                                         <  1:777136/229‑1 (MQ=255)
                                        gCACTGAGTGAAGAGGGTAGTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGt                                                                                                                                                                                          <  1:311209/239‑1 (MQ=255)
                                         cACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATTTCCAGCGTGTCTTGCCCGGTCAGCACTGGTAACAGAGGAGATGGGGATGCAACgcc                                                                                                                                                                                         >  1:59741/1‑237 (MQ=255)
                                              gtggAAGGGGGTTTTGGGGTTTATCCCCGCATTTTCCGCCGAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGc                                                                                                                                                                                     <  2:905341/235‑1 (MQ=255)
                                              aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTCAAAAAGGACTGCATACCCAAAACCTTTGACCCCTACGGGCTGAAATCGAGAACTCCCACCGTGGCTTTCCCCGGCAGCAAAGGGAAAAGGgagaatggggtggcaagtgcgggc                                                                                                                                                                                     >  2:1032414/1‑218 (MQ=255)
                                              aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTTCATACCGAACACCCTGGACCCATGCTGGCAGAAATGCAGAATCTACACCGGGACTTGGCCGGGCCCCAAGCGGAACAAAGGAGAAggggctgaaaatggctctt                                                                                                                                                                                     >  2:777136/1‑223 (MQ=255)
                                              aGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAAAACCTTTGACCCCAGCTGGCAGAAATACAGAATCTCCACCGGGTCTTTCCCCGTCAGCAATGGTAAAAAaggggagggggaacgcaagctgggcc                                                                                                                                                                                     >  2:836354/1‑223 (MQ=255)
                                                   agagGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCCATGGTAACAGAGGGGATGGGGATTCACCGTCTGGCCACCAt                                                                                                                                                                               >  1:421553/1‑239 (MQ=255)
                                                    gggggTATTGGGGTTGTCCCACGCACTTTGCGGGGGGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAGCGAAGCCACGTTGAACGTACCGCAAGAGCAGGGGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTGGGGCCCATGCTGGCAGAAATGCAGTATCTCCGGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTGTGCAACGTCTGGccaccgt                                                                                                                                                                               <  2:253983/236‑3 (MQ=255)
                                                        gTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGGGGCTTGCCCGGGCAGCAATGGTAAAAAAGGAGATGGGGATGCAAAGACTGGCCACCATTgaa                                                                                                                                                                           >  1:905341/1‑236 (MQ=255)
                                                         tATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACa                                                                                                                                                                                                                <  1:288500/201‑1 (MQ=255)
                                                              cgtttgtcccccgccgtttACGCCGGGCGGGGGAGGCCGAAGTTTTGGCCGGTAACAAGGAACCCCAATTGAACGTCCCGCAAGAGCGGGGGTATCGTCCGGGGTGTAAAAAAGGACTGCATACCGAACCCCTTGGACCCATGCTGGCAGAAATGCAGTATCCCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCcac                                                                                                                                                                    <  2:1067767/223‑1 (MQ=255)
                                                                 ttGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGCCTGCATACCGAACACCTTGGACCCATGCGGGCCGAAATGCAGGATCCCCAACCTGTCTTGCCCGGGCAGCACTGGTAACAAAGGGAGTGGGGATTGAACGGTCGGGCCCCCATGCACCCCCACCgg                                                                                                                                                                 >  2:1060332/1‑239 (MQ=255)
                                                                            ctttcccgcgcgGGGGGGGAAGCCAAAGTTTTGGCCGGGATCAACGAAGCCACATTGAACGTCCCGCAAGAGCAGGCGTACCGCACTGGCGGTAAAAAAGGACTGCATACCGAACCCCTTGGCCCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGata                                                                                                                                                     <  1:197721/233‑1 (MQ=255)
                                                                             ttttCCGCGCGGGGGGGGAAGCCAAGTTTTGGGGGGGGAACAAGGAAGCAACATTGACCGTACCGAAAGAGCAGGCGTAGCGCATGGGGGGAAAAAAAGGCCGGAATACCGAACCCCTGGGACCCATGCGGGAAGAAAGGCAGTATCCCCAGGGGGTCGTGCCCGGGCAGAAAGGGTAACAGAGGAGATGGGTAGGAAACGTCGGGCCACCATTGCACCGCCACAGGTTCATGAGatat                                                                                                                                                    <  2:421553/238‑1 (MQ=255)
                                                                                tACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCCCATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTGAAAAAAGACGGGATACCCACAAACACGGACCCAAGCTGGCAGAGATGCGGTATCTACAGCCAGTCTTTCCCGGTCAGACATGGGGAAAGGAGAGATGGGGAAGGCAAGCCTGGCCACCACAGCCCCACcacaaatgacagagaaggggg                                                                                                                                                  >  2:926143/1‑222 (MQ=255)
                                                                                tACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCACCCGGTTCATGAGaaattg                                                                                                                                                  >  1:264494/1‑236 (MQ=255)
                                                                                tACGCGCAGCATGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGGATGCAACGTCTGGCCACCCTTGCACCCCCACAGGTGCATggagtaaggt                                                                                                                                                 >  1:1065245/1‑230 (MQ=255)
                                                                                      cAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTa                                                                                                                                            >  1:148103/1‑238 (MQ=255)
                                                                                        gCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTTCCCGGTCAGCAATGGGAACAGAGGAGATGGGTATGCAACGGCTGGCCACCATTGCACCGCCACCGGGTCATGAGATATGGGCGCTACTc                                                                                                                                         >  1:1073451/1‑239 (MQ=255)
                                                                                        gCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGGAAAAAAGGACTGCACACCCAAAACCTTGGACCCCTGCCGGCAGAAATGGCGTATCGCCAACCTGTCTTGCCCGGTCTGCAAAGGGTACAGAGGAGCTGGGCATGAAACGCATGGGCACCATTTCCCCGCCACAGGTTACCGAGAAAAGGGCGTGACTc                                                                                                                                         >  2:630191/1‑239 (MQ=255)
                                                                                                             tccggggacacaagagcccACATGAACGTTACCGAAAGGGCGGGCGTATCCCCTGGGCGGAAAAAAGGGACTGCACACGAACCCCTTGGGACCCATGCGGGAAAAAAGGCAGTATCCCAGGGGTTTCTTGCCCGGCAAGCAAGGGTAAAAGAGGAGAGGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATcccggacccgga                                                                                                                    <  2:1065245/222‑1 (MQ=255)
                                                                                                                   gATCAACGAACCCCAATTGAACGTACCGCAAGGGCAGGCGTTTGCCATGGGAGGTAAAAAAGGACTGCATCCCGAACCCCTTGGCCCATTGCTGGCAGAAATGAAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGGGGAGATGGGTATGCACCGTCTGGCCCCCATGGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATcc                                                                                                               <  2:148103/238‑1 (MQ=255)
                                                                                                                    aTCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCCTGGACCCATGCTGGCAGAAATGCAGTATCCCCAGCGGGGCTTTCCCGGTCAGCAATGGTAACAGAGGAAATGGGTTTGCCACGTCTGGCCCCCATTGCACCGCCACAGGTTCATGAGATATGGGCGCCACTCAGCCAGAACCCGGACCCGGAGAACCCg                                                                                                             >  2:180326/1‑239 (MQ=255)
                                                                                                                           aaGCCAATTTGACCGTACCGCAAGGGCAGGCGTATCGCACTGGCGGTAAAAAAGGCCTGAATACCGAACACCTTGGACCCATGCTGGAAGAAAGGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCTTTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCt                                                                                                        <  1:755723/237‑1 (MQ=255)
                                                                                                                              ccACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGGATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGGATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGGGATCCCGGCGCTGCCCa                                                                                                   >  1:579212/1‑239 (MQ=255)
                                                                                                                                 caTGGAACGTGCCGAAAGAGCAGGCGTTTCGCACGGGAGGAAAAAAAGGACTGCAACCCGAACTCTTGGGACCCATCCTGGCAAAAATGCATTATCTCCAGCTTCTTTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTAGGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTa                                                                                                <  1:33728/239‑1 (MQ=255)
                                                                                                                                 aaTTGACCGGCCCGCAGGAGCGGGCGTGTCCCCGGGGCGAAAAAAAAGGTCTGCATACCGAACCCCTGGGACCCATGCTGGAAGAAATGAAGTATCCCCAGGTTGTCTGTCCCGGTAAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTa                                                                                                <  2:579212/238‑1 (MQ=255)
                                                                                                                                            aCCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGGATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGGGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAg                                                                                       >  1:1064928/1‑238 (MQ=255)
                                                                                                                                            aCCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGGAACAGAGGAGATGGGTATGCAACGTCTGGCCACCCTTTCACCGCCACAGGTTCATGAGATATGGGCGCTAATCAGCCAGATCCCGGACCCGGGGATCCCGGGGCTGACCCTTACTGGTTTAg                                                                                       >  1:166701/1‑238 (MQ=255)
                                                                                                                                            aCCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTCCATACCGAACACCCTGGACCCATGCTCGGCGAAAAGCAGGATCTCCAGCGTGTCTTGCCCGGGCAGTCATGGTAACAGACGACATGGGGCTGCAACGTCCGGCCACCCTTTCAACCCCCAAGGTTCCATGAATTACGGCGCTCCCCCGCCACACCCCCGGCCCGGCGATTCCCGTGCTACACAATACTGAattcag                                                                                      >  2:204680/1‑236 (MQ=255)
                                                                                                                                             ccGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAACGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGGTCATGAGATATGGGGGCTACTCAGCCCGATCCCGGACCCGGAGATCCCGGGGCTGGCCATTACTGGTTTCGGc                                                                                     >  1:315602/1‑239 (MQ=255)
                                                                                                                                                 aaGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCCGCGTGTCTTTCCCCGTCAGCAATGGTAACAAAGGAGATGGGGATGCACCGTCTGGCCACCATTCCACCGCCCACGGGTCCTGAGATATTGGCGCTAATCCGCCAGATCCCGGACCCGGAGATCCCAGGGCGGACCCTTACTGATTTGGGCAAgg                                                                                 >  2:867610/1‑239 (MQ=255)
                                                                                                                                                      caggtctacccccgtGGCGGAAAAAAAGGACTTCATCCCAAACCCCTTGGACCCATGCTGGAAGAAATGCAGTATCCCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCg                                                                             <  1:180326/224‑1 (MQ=255)
                                                                                                                                                       gggcttcccGCCCTGGGGATAAAAAAGGACTCCATCCCCAACCCTTTGGCCCCTGGCTGGCAGAAATGCATTACCCCCAGCGTGTCTGGCCCGGTAAGCAATGGCAACAGAGGAGATGGGTATGCAACGCCTGCCCCCCATTCCCCCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTa                                                                           <  2:166701/231‑1 (MQ=255)
                                                                                                                                                        ggCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATAAGGGCGCTACCCAGCCAGATCCCGGACCCGGCGATCCCGGGTCTGCCCATTTCTGATTTAGGCATGGGGCGCaa                                                                          >  1:986610/1‑239 (MQ=255)
                                                                                                                                                         gCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCCTGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGc                                                                                                                                                                                                                           >  1:958855/1‑97 (MQ=255)
                                                                                                                                                         gCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGc                                                                                                                                                                                                                           <  2:958855/97‑1 (MQ=255)
                                                                                                                                                               cGCACTGGCGGTAAAAAAGGACGGCATCCCGAACCCCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCGGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGaca                                                                   <  1:836354/239‑1 (MQ=255)
                                                                                                                                                                    cggagatgggtataagaaccaggccGAACCCGTTGTACCCATGCTGGCAGAAATGCAGTATCGCCATCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGa                                                               <  1:838663/215‑1 (MQ=255)
                                                                                                                                                                      gCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTTCCCGGTCAACCATGGGAACAGAGGAGGTGGGGATGCAACGTCTGGGCCCCATTGCACCCCCACCGGGTCATGGGGTATGGGCGCTACTCAACCAACTCCCGGCACCCGGGATCCCGGGGCTGAACCTTACCCATTTAGACATgggggggaaagggaaaaagaggg                                                            >  2:575055/1‑218 (MQ=255)
                                                                                                                                                                          tAAAAAGGGACCGAATACCGAACCCCTTGGACCCATGCGGGCAAAAATGCAGTTTCCCCAGGGTTTCTGGCCCGGTCAGCAATGGTAACAGAGGAGAGGGGTAGGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGgag                                                         <  1:702831/238‑1 (MQ=255)
                                                                                                                                                                          tAAAAAAGGCTTGCCTACCGAACCCGTGGCACCCTGGGTGGAAGAAAGCCAGTTTCCCCAGGGTGTCTGGCCGGTCAGAAAAGGGTAACAGAGGGGAGGGGTAGCCAACGTCGGGCCCCCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGgag                                                         <  2:59741/238‑1 (MQ=255)
                                                                                                                                                                               aaGGCCTGCAAACCGAACACCTTGGACCCATGCGGGAAAAAAGTCAGTATCCCCAGCGTGTCTTGCCCGGTCAGAAATGGAAACAGAGGGGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGAt                                                   <  1:908162/239‑1 (MQ=255)
                                                                                                                                                                                    cTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGc                                                                                                                                                                                                <  1:44762/96‑1 (MQ=255)
                                                                                                                                                                                    cTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGc                                                                                                                                                                                                >  2:44762/1‑96 (MQ=255)
                                                                                                                                                                                            cGAACCCCTTGGCCCCTGCGTGGCAAAAATGCAGTATCCCCAGCGTGTCTTGCCCGGGCAGCAATGGTACCAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCtt                                      <  1:371540/239‑1 (MQ=255)
                                                                                                                                                                                              aaCACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATggg                                                                                                                                                 >  1:797279/1‑131 (MQ=255)
                                                                                                                                                                                                 acCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTTGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGCCCATTACTGATTTAGGCATGGTGCGTAATGGGCACCAGATGGGAGAAGGATGGGAGATCGGCtttcag                                  >  1:431848/1‑235 (MQ=255)
                                                                                                                                                                                                 acCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACCCAGATGGGGGAAGGGAGGGGGATGGGCTTTACGc                                 >  2:665923/1‑239 (MQ=255)
                                                                                                                                                                                                     tGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGCGATCCCGGTGCTGACCTTTACTACTTTGGGCATGGTGCGTAATGTGACACAGCTGGGAGAgggcctgtctcttatcaccatctgcc                              >  2:838663/1‑215 (MQ=255)
                                                                                                                                                                                                       tgggccccgtggggggaaaaaTGGATTAGCCCCAGCGTGTCTGCCCGGGACGAAATTGGGAAGAGAGGAGAGGGTTATCAAACTTCTGGCCCCAATTGGCCCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGa                              <  2:986610/221‑1 (MQ=255)
                                                                                                                                                                                                       ggACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACa                            >  1:114041/1‑239 (MQ=255)
                                                                                                                                                                                                              aTGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTcc                      >  1:889600/1‑239 (MQ=255)
                                                                                                                                                                                                               tGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCACTGGCAACAGAGGAGATGGGGATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGGAATGTGCCACAGATGGGGGAAGGATGGGGGATCGGCTTTCCGCCGACATTTTCCg                     >  1:1001305/1‑239 (MQ=255)
                                                                                                                                                                                                               tGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCCTTACTGATTTAGGCATGGTGCGTAATGTGACACCGATGGTCGAAGGATGGGTGACCGGGTTTCCCCCCCCACATTCCg                     >  2:956922/1‑239 (MQ=255)
                                                                                                                                                                                                               tGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGGGATCGGCTTTACGCCGACATATTCCg                     >  1:803852/1‑239 (MQ=255)
                                                                                                                                                                                                               tGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGGAATGTGACACCGATGGGAGAAGGATTGGTGATCGGCTTTAGGCCGACGAATTCCg                     >  2:220319/1‑239 (MQ=255)
                                                                                                                                                                                                               tGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGGGCGTAATGGGACACAGATGGGGGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCg                     >  1:440048/1‑239 (MQ=255)
                                                                                                                                                                                                               tGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGGGCTGACCATTACTGATTTAGGCATGGGGCGTAATGTGACACAGATGGGAGAAGGGTGGGGGATCGGCTTTACGCCGACATATTcgg                     >  1:974264/1‑237 (MQ=255)
                                                                                                                                                                                                               tGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGAATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGACGGATGGGTGAACGGCTTTACGCAGACATATTACg                     >  1:10008/1‑239 (MQ=255)
                                                                                                                                                                                                                            tGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAAt                                                                         >  1:748712/1‑175 (MQ=255)
                                                                                                                                                                                                                                   tctcCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACa  >  1:465263/1‑239 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
TTTTACCGCCGAACTGTTCGATGCCGACGAGATTGATATTGCACTGAGTGAAGAGGGTATTGCGGTTGATCCACGCACTTTACGCGCAGCGTGGGAAGCCGAAGTTTTTGCCGGGATCAACGAAGCCACATTGAACGTACCGCAAGAGCAGGCGTATCGCACTGGCGGTAAAAAAGGACTGCATACCGAACACCTTGGACCCATGCTGGCAGAAATGCAGTATCTCCAGCGTGTCTTGCCCGGTCAGCAATGGTAACAGAGGAGATGGGTATGCAACGTCTGGCCACCATTGCACCGCCACAGGTTCATGAGATATGGGCGCTACTCAGCCAGATCCCGGACCCGGAGATCCCGGTGCTGACCATTACTGATTTAGGCATGGTGCGTAATGTGACACAGATGGGAGAAGGATGGGTGATCGGCTTTACGCCGACATATTCCGGTTGTCCGGCAACGGAACA  >  NC_000913/1455655‑1456115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: