| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,727,771 | C→G | 16.1% | intergenic (+15/‑66) | nemA → / → gloA | chromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme/glyoxalase I, Ni‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,727,771 | 0 | C | G | 16.1% | 152.2 / 3.8 | 76 | intergenic (+15/‑66) | nemA/gloA | chromate reductase, quinone reductase, FMN‑linked, N‑Ethylmaleimide reductase, old yellow enzyme/glyoxalase I, Ni‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (44/16); new base G (5/10); total (50/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGAAC > NC_000913/1727537‑1728005 | tttCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCC‑GCAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAgcggcg > 1:653500/1‑239 (MQ=255)tttCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGACGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTAAGCTTAAACACCAGCGTGCC‑GAAAGTTTCTACGGTGTCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTACTCCAACATTGCCGgcggcg > 1:495624/1‑239 (MQ=255) cgttccgttttggggggagggggCATACAAAGTAGAAAAATGGGAAACGCTGGTGGGCAAAGGG‑TTAATTGGTGGGGTGGCATTTGGTCGTGACTGGATTGCGAACCGGGATCGGGTCGCCCGCTTGCAGCGCAAAGCTGGGCTTACCCCACAGCGTGCC‑GAAAGTTTCTAGGGTGGCGGCGCGGAAGGTTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAgc < 2:609697/217‑1 (MQ=255) tCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGGGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CACTTACCAGACGTTGTAATCAAACATTGCGACCGGCGTAAAgccg > 1:230109/1‑238 (MQ=255) cGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAgcgccc > 1:149530/1‑234 (MQ=255) ggCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAACGTTTTTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGCTTACCCCACGTTTTAATCCAACATTGCGAGGGGCGGaaaacgccgcccatacta > 2:654757/1‑224 (MQ=255) agcaGAAAACGCTGAAACGATGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGGGACTGGATTTGGAACACGGATCTGGTCGCCCGGTTGCAGCGCAACGCTGAGCTTATCGCCCAGCGTGAC‑GAAAGTTTCAACAGTGGCGGCGCGGAAGGCGATC‑‑C‑‑CGATTACCCCACGTTCTAAACCACACTTTCGAGTGGGGTCACGGCGCCGCAAGCAAAGAACAACATTTTGAAt > 2:713507/4‑238 (MQ=255) aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTCATCCAACATTGCGAGCGGGGGAAACCCGCCCCTCTACTAAAAAAAAATTTTaaa > 2:757579/1‑237 (MQ=255) aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGaa > 1:13508/1‑237 (MQ=255) aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGAGCGGACGGCTATA‑‑CCACGATTACCCGACGTTGTAATACAACATTGCGACGGGCGTAAAACCGCCGCCATAATaaaaaacaaatttaaa > 2:347407/1‑227 (MQ=255) aGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑GTAATTGATGCGGTGGCATTTGGTCGTGACTGGCTGGCGAACCCGGATCTCGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGACGCGGAATGCCATA‑‑C‑‑CGCGTACCCGAGGCTTCACTACAAAATTGCGGCGGGGGTCAAGCCCCCGGTCTACCAAATCAAAATATTTaaca > 2:154984/1‑237 (MQ=255) gTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACCGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTCTA‑‑C‑‑CGATTACCCGCCGTTGTAATACAACATTGGGAGGGGCGTAAAGCCCCGCCTTTAGTAAAAAAAAATTTTGaa > 2:637775/1‑236 (MQ=255) gTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAAAATTTTGAATCt > 1:466496/1‑239 (MQ=255) tAGAAAACGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCACAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGATCCTACGGTGCCGGCCCGGAAGGCAATA‑‑C‑‑AGTTTACCCGACGTTGTATTCCACCAATTCGAGGGGCGTAACGCCCCCCCCA‑ACATAAACAACAattttacatact > 2:157639/1‑231 (MQ=255) tAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGaa > 1:259358/1‑235 (MQ=255) tAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGACGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGCCGTTGGTATCCAACATTGCGGGCGGCGTAAAACCCCCCCAATACAAAAAAAAAATTTTaaacagt > 2:369271/1‑235 (MQ=255) aaaaaaaCGTGAATCGCTGATCGGCAAAGGG‑TTAATTGATGGGGTGGCATTTGGTCGGGACTGGTTTGCGAACCGGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGt < 1:1032039/237‑1 (MQ=255) aaaGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAg > 1:192535/1‑238 (MQ=255) aaGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTTTAATCCAAAATGTGGAGCGGCGTAAAGCCCCCCCTTTACTAAAAAAAAATTTTAAAATTGTTAGcc > 2:843146/1‑239 (MQ=255) tGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGTAAGGCTATA‑‑C‑‑CGATTACCCGCCGTGGTAATCCAACACTTCGAGCGCCGTAAAGCCGCCGCAATACTAAAACACCATTTTTAATcagataacaatat > 2:20583/1‑227 (MQ=255) tGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACCTTTTGAATCTGTTAGCCAttt > 1:463855/1‑239 (MQ=255) gaacccgttgtgggaaaGGGTTTAATTGGTGCGGGGGCTTTTGTGCGTGAGTGGTTGCCGAACCCGGATCGGGCCCCCCGCTGGCAGGCAAAAGCTAGGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGGGGGGGCGCGGAGGGCTATA‑‑C‑‑CGATTCCCCGACGTTGTAATCCAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCAtttt < 2:610005/225‑1 (MQ=255) aaaCGCTGATCGGAAAAGGG‑TTAATTGATGCGGTGGCATTTGGGCGGAGCTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCAtttt < 1:349873/238‑1 (MQ=255) aaCGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAAAAACATTTTGAATCTGTTAGCCATTTTGa > 1:356459/1‑239 (MQ=255) aTCGGCAAAGGG‑GTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCAGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GCAAGTTTCTACGGTGGGGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGCCGTTGTAATCCAACATTGCGGGCGGCGTAACGCCGCCGCCATACTAAAACAACATTTTTAAACTGTAAGACATTATGGGGAaaaa > 2:109790/1‑239 (MQ=255) g‑TTAATTGGGGCGGTGGCATTTGGTCGTGCCTGGATTGCGAACCCGGATCTGGTCCCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCAAAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATC‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt < 1:654757/237‑1 (MQ=255) g‑TTAATTGATGCGGGGGCATTTGGTCGTGACTGGATGGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCGCAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt < 1:709118/237‑1 (MQ=255) ttttgttgtggCGGTGGCATTTGGTCGTGAGTGGAGTGCGCACCTGGATCTGGGCCCCCGTGTGCGGGGCAAAGCTGAGTTTAACCCACAGCTGGCA‑GAATGTTGTGACGGTGGCGGGGGGGAAGGTTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGtc < 2:168603/228‑1 (MQ=255) atttgttgcgtgtgcttttGGTGGTGAGTGGGTTGCGACCCCGGATCTGGTCCCCCGCTTGCAGCGCAAAGCTGGGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGtcttct < 2:259358/223‑1 (MQ=255) tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGGGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTCGGGATAAAAAGATCCGGATTCTTCaaa > 2:166816/1‑236 (MQ=255) tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATa > 1:762829/1‑238 (MQ=255) tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAAAATTTTGAATCAGTTTGCCATTTTGAGGATAAAAAAATGCGGctttttacaac > 2:761231/1‑234 (MQ=255) tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCCTTACTAAAAAAACATTTTAAATCTGTTAGCCCTTTTGGGGATaaaaaaaggggcctctttcaaac > 2:867569/1‑221 (MQ=255) tGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGGAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTAGATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTTTTCAAAc > 1:674119/1‑239 (MQ=255) gCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATAcc > 1:26671/1‑239 (MQ=255) ggCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACACCATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATActctgcg > 1:729194/1‑233 (MQ=255) gggtgttGACTGGATTGCGAACCCGAATCTGGTCGCCCGCTTGCCGCGCAAAGCTGAGCTTAACCCACAGGTTGCC‑GAAATTTTCTACGGTGGCGGCGGGGAAGGCTATA‑‑C‑‑CGATTACCCGACTTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGt < 1:843146/233‑1 (MQ=255) gtgacgtgttgcggccccggtttCTGGTGCCCCGTTTGCGGCGCAAGGCGGAGCTAAACCACCAGCGTCCC‑GAAGGTTTCGAGGGGGCGGGCGGGGAAGGCTATC‑‑C‑‑CGATTACCCGACTTTGTAATCCAACATGGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCg < 2:13508/217‑1 (MQ=255) tGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACCTTTTGAATCTGTTACCCCTTTTAGGGGTAAAAAAATGCGTCTTCTTCAAACAATCA‑TGCGCGTTGGCGa > 2:144461/1‑239 (MQ=255) tGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAAAAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGCCTTCTTCTTCCCATGC‑TCCGCGTTGGCGa > 1:924733/1‑239 (MQ=255) aTTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGACTCTGTTAGCCATTTTGTGGATAAAAAAATGCGTCTTCTTCATACCAAGC‑TGCCGGTTTGCGATTTGCaa > 2:30694/1‑239 (MQ=255) aTTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAAAATTTTGAATCTGTTAGCCATTTTGAGGTTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTCGGCGATTTGCa > 1:648123/1‑238 (MQ=255) ccGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTTCAACGCTCCATc > 1:737176/1‑239 (MQ=255) cagtctttggccccccGCTTGAGCCGCAAAGGTAGGCTTACCCCCCCG‑GTGCCGGAAAGTTTGTGGCGTGGGGGCGCGAAAGGTTATT‑‑C‑‑CGTTTCCCCGACTTTGTAATCCACAATTGCGGGGGGCTTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATc < 2:332645/226‑1 (MQ=255) tttggcccccccCTTGCAGCGAAAAGCGGGGTTAACCCCCCGGCGGGCC‑AAAAGTTTCTCCGGGGGCGGGCGGGAAGGCTATA‑‑C‑‑CGATTCCCCGACGTTGTAACCAAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGAttt < 2:924733/231‑1 (MQ=255) gTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GACAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTAGAGGATAAAAAGGTACGTCTTCTTCAAACCATGC‑TGCGCGTTGGCGATTTGCACAGATCCATCGGTTTTtat > 2:479834/1‑239 (MQ=255) gTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGTTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCCCGTTGGCGATTTGCAACGccttctccttttaacc > 1:818029/1‑224 (MQ=255) tCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAaaca < 1:854751/141‑1 (MQ=255) tCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAaaca > 2:854751/1‑141 (MQ=255) ccGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCGACGCTCCATCGATTTTTATACCaa > 1:820562/1‑239 (MQ=255) cTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTTCGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTCAAGCCGCCGCTATACTAAAACAACCTTTTGAATCTGTTAGCCATTTTGCGGATAAAAAGATGCGACTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGGTTTTTATACCAAAGt > 1:285886/1‑239 (MQ=255) ggcgcgaAAAGGTGGGTTTACCCCACAGGGGGCC‑AAAGGTTTCGACGGGGGCGGGCGGGAAGGTTAAC‑‑C‑‑CGATTCCCCGACTTTTAAATCCAACATTGCGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCt < 2:703272/236‑1 (MQ=255) gaaaggcggggCTTAACCCCAGGGGTGCC‑AAAAGTTCTAAGGGTGGCGGGCGGGAAGGTTATC‑‑C‑‑CGATTCCCCGACGTTGTAACCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCa < 2:653500/229‑1 (MQ=255) aaCCCACAGCGCGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGCTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGTATGAAATGGCTGCGt > 1:312690/1‑239 (MQ=255) acaGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGCTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGCGGATAAAACGATGCGTCTCCTTC‑TTCCATTCATGCGCGTTGCCGGTTTCCACCCCTCCCTCGTTTTTTTTCCCAAAGGACTGGGGATGAAActcccactaacaag > 2:293566/1‑227 (MQ=255) acaGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAGACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGCTTTGCAACCCCCCATCCATTTTTTTACCAAAGTGCTGGGCATGAAACTACTGCGTACCac > 2:212298/1‑238 (MQ=255) acaGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCa > 1:128133/1‑238 (MQ=255) tGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTTTACCAAAGTGCTGGGCATGAAAAGGCTGGGGACCAACGaaaa > 2:215964/1‑239 (MQ=255) aaattttttcggggggggggggggAAGGTTTTA‑‑C‑‑CGTTTCCCCGAGTTTGAAATCCAACATTGGGAGGGGCGAAAGGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCgg < 2:463855/217‑1 (MQ=255) aaaGTTTCTCGTGTGGGGGCGCGAAGGGATATC‑‑C‑‑CGATTCCCAGACGTTGTAATCCAAAAGTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCgg < 1:582943/239‑1 (MQ=255) atttttcagcgggggcgcgggggAAGGTTATC‑‑C‑‑CGTTTCCCCGACGTGTTAATCCAACATTGAGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCgg < 1:43638/217‑1 (MQ=255) gtttttcgggggggggggcgggAGGGTTATA‑‑C‑‑CAATTCCCGGACGTTTAAATCCAAGATGGCGAGGGCGGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGaa < 2:149530/219‑1 (MQ=255) tttggtcgggggggggggggAAGGCTTAC‑‑C‑‑CGTTTCCCCGACGTTGAAAACCAACATGGGGAGGGGCGTAAAGCCGCCGGTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGaa < 2:128133/219‑1 (MQ=255) cTACGGTGCCGGCCCGGAAGGCTATACCC‑‑CAATTCCCCGACGTTTTAACCCAAATTGGCGGGCGGCGTAAACCCGCCGATATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATAc < 1:347407/239‑1 (MQ=255) tGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGCTAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCCTGAAAATGCTGCGTACCAGGGAAAACCCGGAATACAAAAACTc > 2:832500/1‑239 (MQ=255) ggcgGGTAAGGCTATC‑‑C‑‑CTTTTCCCCGACTTTGTTATCCAATCTTGGGAGGGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTg < 2:356459/239‑1 (MQ=255) gcgcGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt < 1:426194/121‑1 (MQ=255) gcgcGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt > 2:426194/1‑121 (MQ=255) aTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGcc > 1:495802/1‑239 (MQ=255) aTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAAAACTCACTGGCGTTTGTTGGCTACGGcc > 2:604423/1‑239 (MQ=255) tACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCg > 1:490713/1‑239 (MQ=255) aCGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAAcc > 1:1034825/1‑239 (MQ=255) atcagtaaTTGCGGGCGGCGTAAAGCCGCCGTTATACTAAACCAACATTCGGAATCTGTTAGCGATTTTGAGGATAAAAAGATGCGTCTTCGTCATACCATGC‑TGCGCGGTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAAGACAAATACTCACTGGCGTGTGTTGGCTACGGCCCGGAAACCGAAGAAGc < 1:215964/232‑1 (MQ=255) aCATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGGGa > 2:510359/1‑239 (MQ=255) cATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGAt > 1:310391/1‑239 (MQ=255) cATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAAAAACGGGGaa > 2:185367/1‑238 (MQ=255) gCGAGCGGGGTAAAGCCGCCGGTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGaa < 1:791857/239‑1 (MQ=255) gAGGGGCGAAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGAAc < 1:637775/238‑1 (MQ=255) | TTTCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGG‑TTAATTGATGCGGTGGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCC‑GAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATA‑‑C‑‑CGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGC‑TGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTGCGTACCAGCGAAAACCCGGAATACAAATACTCACTGGCGTTTGTTGGCTACGGCCCGGAAACCGAAGAAGCGGTGATTGAAC > NC_000913/1727537‑1728005 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |