Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,901,015 T→C 21.5% S191G (AGC→GGC)  yoaE ← putative membrane protein/conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,901,0150TC21.5% 127.7 / 5.3 62S191G (AGC→GGC) yoaEputative membrane protein/conserved protein
Reads supporting (aligned to +/- strand):  ref base T (26/20);  new base C (4/10);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATG  >  NC_000913/1900781‑1901246
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
cggcggtgtgtggAACATTGGCCTGACGTTCCCCGCCAACCAAACGCAGGATGGCATCGGCAGTACGGGCGCGCAGCGGCAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAAGCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                           <  2:174483/227‑1 (MQ=255)
cGGCATCGTGCTGAACATGGGCCTGGCGTTGCCCGCCCAACAACCGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTAGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                           <  1:380947/239‑1 (MQ=255)
cGGCAGCGTGCTGAACATGGGCCGGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcaga                                                                                                                                                                                                                                           <  1:635721/239‑1 (MQ=255)
        gtgggaccacgggCCCGGCGTTTCCCGCCCATCAAACGCAGGATGGGATCGGCATGACGCGCGCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGGGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGcac                                                                                                                                                                                                                                  <  2:550655/228‑1 (MQ=255)
             aaCATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGGGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGGTGACACCGAAACCTTCTGCCACCCACCACAAACCCATCATTAACAGGAAGCCccgacagaaccacacc                                                                                                                                                                                                                              >  2:793634/1‑230 (MQ=255)
              aCATTGGCCTGACGTTTCCCGCCCAGCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAACCCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGcaccacca                                                                                                                                                                                                                            <  1:690443/238‑1 (MQ=255)
                  tGGCCTGGCTGTTCCCGCCCAGAAAACGCAGGAGGGCATCGGCAGTACGCGCTCGCAGGGGAAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTg                                                                                                                                                                                                                       <  2:82674/239‑1 (MQ=255)
                  tGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCCTACAGGAAACCTTTCGGAATTTGGAAACCGAAACCTTCTCCCCCCAGCACCACACCCACCCTTAACAGAGAACCCACACAGAgaccaccaaccccg                                                                                                                                                                                                                      >  2:50478/1‑233 (MQ=255)
                  tGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACCCAGCCCAACCATTAACAGGAACCCCAAAAAGAACACCACCACCCTg                                                                                                                                                                                                                       >  2:1042752/1‑239 (MQ=255)
                       tgaactttCCCGCCCATCAAACGGAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGGCGACTGGGGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTg                                                                                                                                                                                                                 <  1:974699/234‑1 (MQ=255)
                       tGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCCGCCTCAGACCAATCATTAACAGGAAGCTCAGACAGGGCACCACCACCGAGGGGt                                                                                                                                                                                                                  >  1:844227/1‑238 (MQ=255)
                       tGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCCGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCCAACCCAAACCAATCATTAAAAAGAAACCCAGACAAAGCACCCCCACCGTgggggg                                                                                                                                                                                                                 >  2:1046396/1‑237 (MQ=255)
                         aCGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCCGACTCAGACCAATCATTAACAGGACGCTCAGACAGAGCACCACCACCGCGGGGt                                                                                                                                                                                                                  >  1:807064/1‑236 (MQ=255)
                         aCGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAAAAGGAAGCTCAGACAGAGACCCACCACCGGGGGGGGcg                                                                                                                                                                                                               >  1:928256/1‑238 (MQ=255)
                                   cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCACTCATTAAAAGGAAGCTCCGACAGAGCACCACCACCGTGGGGTGCTGGGTAACGa                                                                                                                                                                                                      >  1:163340/1‑238 (MQ=255)
                                   cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTcagacag                                                                                                                                                                                                                                       <  1:76467/207‑1 (MQ=255)
                                   cccATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAAGCCAATCATTAACAGGAAGCTcagacag                                                                                                                                                                                                                                       >  2:76467/1‑207 (MQ=255)
                                        cAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGGTGATCGAGAAGCCAATCGCGGCATACAGGGAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGAACCACCCCCGTGGGGTGCGGGCTAACGAACcgcg                                                                                                                                                                                               >  1:667632/1‑239 (MQ=255)
                                          aaCGAAGGATGGGATCGGCAGTTAGCGACCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATTGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTc                                                                                                                                                                                             <  1:1083115/239‑1 (MQ=255)
                                                    ggCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTgg                                                                                                                                                                                   <  1:284403/239‑1 (MQ=255)
                                                         cGGGAGTAGGCGCGCGGAGTGGGAAAGGCCGGTGGTGGCGAACAAAGTGGGGACGCGCAATCTGGTTAAACACTTCGAGGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGcc                                                                                                                                                                              <  2:446597/239‑1 (MQ=255)
                                                            cAGTACGCGCTCGCAGGGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAACCCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCTCCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAg                                                                                                                                                                            <  1:793634/238‑1 (MQ=255)
                                                                   cggtCGCAGCGGCAAAGGCGACTGGTGGCGAATAAAGTTGCGACGCGCAATTTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACAt                                                                                                                                                                      <  1:1046396/234‑1 (MQ=255)
                                                                        ggagcggaaatgtttgttgggggCGAAAAAAGTTCCGACGCCCAATCTGTGAAAACCTTTGGATGCTGACCGAGAAGCCAACCGCGCTAAACAGTGACCCTTTCGAAATGTGGAAACCGAAACCTTCTGCCACCAGACCCAGACCAATCATTAACAGGACGCTCAGACAGAGCACCACCCCCTTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATTCCAGCAACATAACCGcc                                                                                                                                                               <  2:921072/222‑1 (MQ=255)
                                                                             gggaaaGTCGACTGGGGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAAAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATc                                                                                                                                                            <  1:1084446/234‑1 (MQ=255)
                                                                                           gtggCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCACCGGTTTGGATGCCAGCACCATAACCCCCATCGCAAtacatacctcc                                                                                                                                             >  1:953057/1‑228 (MQ=255)
                                                                                              gcgactactattGCGACGCGCACTCTGAGTAAACACTTCAATGATGATCGAGACGCCAAACGCGGCATACAGGTAACCTTACGGTATGAGGCAACCGGAACCTTCTGCCACCAGACTCAGACCAATCATAAACAGGAAACTAAGACAGAGCAGCACCACGGCGGGTTGTTGATTACTGACGAGCACCAGCGGTTTGGATGCCAGCAACATAACCGCAACCGCAAACAAAAccgcgccca                                                                                                                                         >  2:125729/11‑234 (MQ=255)
                                                                                              gCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGGGCTGGTTAACGAATCGCGTCAACGGTTTGGATGCACGCAAAATAACCGCCATCGCAATCACAACCGCCCCca                                                                                                                                         >  2:533983/1‑239 (MQ=255)
                                                                                                                 gtaaTTTGGTTAACCACTTCGATGATGATGGAGAAGCCAACCGGGGCATACAGGTAACCTTTGGGAATGTGGAACCCGAAACCTCTGGCCACCAGACCAAGACCAATCATTAACAGGAAGCCCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGt                                                                                                                       <  2:933631/236‑1 (MQ=255)
                                                                                                                          ttAAACACTTCGATTATGCGCGGAAAGCCAATCGCGGCATACAGGTACCCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGCCTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTGGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATccc                                                                                                             <  1:1085250/239‑1 (MQ=255)
                                                                                                                            aaccccTTCGATGATGAACGAAAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGGGGAAACCGAACCCTTCGGCCACCAGACTCAGACCAATCATTAACAGGAAGCCCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  1:915017/234‑1 (MQ=255)
                                                                                                                            aaaccttgcggggattgaggaaaaGCCACCCGCGGCATACAGGAAACCTTTCGGAATGGGGAAACCGAAACCTTCGGCCACCAGACTCAGGCCAATCATTAACAGAAGGCACAGACAGAGCACCACCACCGGGGGGTGCTGGTAAACAAATCGCGTCAGCGGTTTGGATGCCAGCAACTTAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  2:819460/217‑1 (MQ=255)
                                                                                                                            aaaacctatggttatgcacGAAAAGCCAATCCGGCAAAAAGGGTACCCTTCAGGAATGGGAAACCCGAACCCTTCCACCCCCAACCCCAGACCAACAATTAAAAGGAACCCAAGCAAGAGCCCCACCCCCGGGGGGTGCTGGTTAACGAATCGCGTCAGCGTTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  2:760089/221‑1 (MQ=255)
                                                                                                                            aaaCACTTGGATGATTAACGAGAACCCAATCGGGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCCGCCCCCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGGGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTa                                                                                                           <  1:867782/239‑1 (MQ=255)
                                                                                                                             aaCACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCCCTACCGCCGCCATCATCACCGGCAGATGGTTAACCATcccaa                                                                                                           >  1:527950/1‑236 (MQ=255)
                                                                                                                                   tCGCTGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCCGt                                                                                                    >  1:967841/1‑239 (MQ=255)
                                                                                                                                   tCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGt                                                                                                    >  1:1006214/1‑239 (MQ=255)
                                                                                                                                          ggataggaaaGCAAATCGCGGAATACAGGTAACCTTCCGGAATGTGGAACCCGAAACCTTCTCCCCCCAGACTCAGACCAATCATTACCAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGAATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTAc                                                                                              <  1:1042752/230‑1 (MQ=255)
                                                                                                                                                 aaggacccgccggcaaaaaaGGTAACCTTCGGAAATGGGGAAACCGAAACCTTCCGCCCCCAAACCCAGCCCAATCATTAACGGAAAGCTAGGACAGAGCACCACCCCCGGGGGGTGCTGGTTAACGAAGCGCGTCAGGGGTTTGGATGCCAGCAACAAAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATcc                                                                                       <  2:834496/219‑1 (MQ=255)
                                                                                                                                                       aaTCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACCACCGCCGCCACCATCCCCGGCAGAGGGTTAACCACCCCAACCCCAGAAAATACCCACTCCAAA‑‑gaga                                                                              >  2:995618/1‑239 (MQ=255)
                                                                                                                                                         tCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCACATGGTTAACCATCCCTACTGCAGTAATTACCCCACCCAAC‑‑Gaaaaa                                                                            >  2:154342/1‑238 (MQ=255)
                                                                                                                                                           ccggctcctcatgTAACTTTCAGGAATGTGGAACCACAACCCCTCCGCCCCCGGCACCATCCCAACAATAAACAGGACGCCCAGACATGGACCCTCCTCCGTGGGTGTGGGGTTAACGACTCGCGTGAGCGGTTTGGATGCCAGCAACTTAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑Gagaaga                                                                           <  2:1052259/227‑1 (MQ=255)
                                                                                                                                                                        ttcccTTTCGGAATTGGGAAACCGAACCCCTCCCCCACACACCTCAGACAAACCTATAACGGGACTCCCAGACAGACCACCCCCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGTTTTGGATCCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGAt                                                              <  2:928256/236‑1 (MQ=255)
                                                                                                                                                                        tAACCTTCAGGAATGTGGAAACCGAACCCTTCTGCCCCCCGCCTCAGACCAATCATTAACAGGAAGCCCAGCAGGAGCACCACCCCCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAgg                                                                <  1:533983/237‑1 (MQ=255)
                                                                                                                                                                         taCCCTTCCGGATGTGGAAACCCGAAACCTCCTGCCACCAGACCAAGCCCAACCATAACCAGGAACCCAAGACGAGCCCCACCCGCCGTGGGGTGCGGTTTAACGAATCGGGCCGCGGGTTGGGATGCCACCAACATACCCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGAt                                                              <  2:809005/238‑1 (MQ=255)
                                                                                                                                                                          aCCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCCTCATCACCGGCAGATGGTTAACCATCCCTCCTGCAGTAATTACCGCATCCAAC‑‑GCGAAGCCGGctcacaggatg                                                             >  1:576117/1‑228 (MQ=255)
                                                                                                                                                                            cTTTCGGAGATGTGGAAACGAAACCCGTGTCCCACCGCACCGAGCCCAATAATGAACAGGAGACGCGGAAAGGGCCCCCCCCCGGGGGGGTGCGGGTTAACGAATCGGGTCAGCGGTTGGAATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGAc                                                           <  2:501251/239‑1 (MQ=255)
                                                                                                                                                                               tCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGCTGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAAACGACGTCAAGGAAGACaa                                                         >  1:539455/1‑237 (MQ=255)
                                                                                                                                                                               tCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGCAGGCGTCAAGGAGGACaacc                                                       >  2:691727/1‑237 (MQ=255)
                                                                                                                                                                               tCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑ACAAAGAAGGCGTCAACGATGCCaa                                                         >  1:330698/1‑237 (MQ=255)
                                                                                                                                                                               tCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCCACGTCAGAAATTACCCCCTCCAAC‑‑GAAAAAACGGCgcaaagaaagaaaaaa                                                       >  2:887703/1‑224 (MQ=255)
                                                                                                                                                                                             acccaacccctccgccccccaGACGCAAACCAAACACAAACAGGAGGCCCAGACAGGCCACCCCCCCCGTGGGGGGCGGGTTAACTAACCCCGTCAGGTGTTGGAATTCCAGCAATATACCCCCCATACCATTCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACGGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTgacgac                                            <  2:539455/220‑1 (MQ=255)
                                                                                                                                                                                               ccgaaaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGGTAACCATCCCTACTGCAGCAATTACCGCATCCAAC‑‑GAGACGACGGCGTCAAGGATGCCAATCttgttgacgccac                                          >  1:111425/1‑226 (MQ=255)
                                                                                                                                                                                                   aaccTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGCAGGCGTCAAGGTTGACAATCTGTGTGCCGACCACCCaa                                     >  1:378853/1‑238 (MQ=255)
                                                                                                                                                                                                          tGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTg                               >  1:212925/1‑238 (MQ=255)
                                                                                                                                                                                                           gccccaggACTCAGCCCAATCATTAACAGGAAGCTCAACACGGCCACCACCACCGTGGGGTGCTGGTTAACGAACCGCGTCAGCGTTTTGGATGCCAGCAAAATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGc                             <  1:691727/232‑1 (MQ=255)
                                                                                                                                                                                                                        gACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACaa                                                         >  1:885940/1‑198 (MQ=255)
                                                                                                                                                                                                                            cacaagtAACGGGAATCCCAGACGGGCCACCACCCCTGTGGGGGGTGGGTAAAGAAACGGCGTAGGCGTGTTGAAGCGCACCAAAATACCCGCCATCGCAATCACTCCCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGc            <  2:967841/233‑1 (MQ=255)
                                                                                                                                                                                                                            aaccATTAACAGGAAGCCAAGACAGGCCACCACCCCGGTGGGGTGCTGGTTACAGAACCGCGTCAGCGGTTTGGATGCCAGCAACATACCCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGc            <  1:180152/236‑1 (MQ=255)
                                                                                                                                                                                                                             aTCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCACC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCCCCCAGAAACCGGCGTACCCTTTACCTTGGcg           >  1:1054442/1‑238 (MQ=255)
                                                                                                                                                                                                                                     cAGGCAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTACTCACCGCATCCACC‑‑GAGAAGACGGCGTCAAGGATTACAATATTAGGGACGACCACCCCGAAACCGGCGTAACCTTTTACGAGGCCgggaacaa   >  2:188651/1‑233 (MQ=255)
                                                                                                                                                                                                                                        gAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAACGAGAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGCCGTAGCCTTTACCGTGGCCGGAAACATg  >  1:995959/1‑239 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                             
CGGCATCGTGCTGAACATTGGCCTGACGTTTCCCGCCCATCAAACGCAGGATGGCATCGGCAGTACGCGCTCGCAGCGGCAAAGTCGACTGGTGGCGAATAAAGTTGCGACGCGCAATCTGGTTAAACACTTCGATGATGATCGAGAAGCCAATCGCGGCATACAGGTAACCTTTCGGAATGTGGAAACCGAAACCTTCTGCCACCAGACTCAGACCAATCATTAACAGGAAGCTCAGACAGAGCACCACCACCGTGGGGTGCTGGTTAACGAATCGCGTCAGCGGTTTGGATGCCAGCAACATAACCGCCATCGCAATCACTACCGCCGCCATCATCACCGGCAGATGGTTAACCATCCCTACTGCAGTAATTACCGCATCCAAC‑‑GAGAAGACGGCGTCAAGGATGACAATCTGTGTGACGACCACCCAGAAACTGGCGTAGCCTTTACCGTGGCCGGAATCATG  >  NC_000913/1900781‑1901246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: