Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,029,277 C→T 14.7% A30A (GCG→GCA yedI ← DUF808 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,029,2770CT14.7% 171.4 / 3.7 77A30A (GCG→GCAyedIDUF808 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (8/56);  new base T (10/2);  total (18/59)
Fisher's exact test for biased strand distribution p-value = 2.39e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAAC  >  NC_000913/2029044‑2029510
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
aCTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAAAACACCGGCGGGTTTTCTTcccc                                                                                                                                                                                                                                             >  2:510411/1‑238 (MQ=255)
    tctcTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTCgccgccc                                                                                                                                                                                                                                         >  1:772421/1‑236 (MQ=255)
    tctcTACTCCATCAAAGCAAAGAAACGCCCCACCAACCAGCAACAGGGGCGTAATCGCCCACGGGATGAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCCCTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCGGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGt                                                                                                                                                                                                                                       <  2:151440/239‑1 (MQ=255)
         aCTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGGTTTTTTTgcccccccgtttc                                                                                                                                                                                                                                   >  1:604781/1‑231 (MQ=255)
         aCTCCTTCAAAGCAGAGAAACGCGCCACCAATCAGCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGcc                                                                                                                                                                                                                                 <  1:266104/239‑1 (MQ=255)
            ccTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCAAGCGGCACCAGAATCACTTTATTAATAAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGGTAAGTCATCCCCTAATACACCGGCGGTTTTTTTTCCCCCCAGATTTcccc                                                                                                                                                                                                                               >  1:730457/1‑237 (MQ=255)
               tCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGCTTTTTTTTCCCGCCGTTTGTCCCTCa                                                                                                                                                                                                                           >  1:783697/1‑238 (MQ=255)
                cAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAATCTTGTTGCGCATTGAGCGCTAAGTCATCCCCTAATACACCGGCGGTTTTTTTTTCCCCCCCTTTGTCCAtacac                                                                                                                                                                                                                          >  2:341737/1‑235 (MQ=255)
                      cgagaAACGCGCCACCAACCCTCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCCCTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGata                                                                                                                                                                                                                  <  1:454898/238‑1 (MQ=255)
                      cgaaaaaCGCCCCACCAATCACCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGata                                                                                                                                                                                                                  <  2:627825/235‑1 (MQ=255)
                      agagAAACGCGCCACCAATCACCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGata                                                                                                                                                                                                                  <  1:954704/239‑1 (MQ=255)
                       gagaAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGGTTTCTTTTCCGCCCGTGTTGccacccccgagcaa                                                                                                                                                                                                                     >  1:401108/1‑226 (MQ=255)
                       gagaAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGGTTTCTTCGCGCCCCGCTTGCCCaccccccagaaa                                                                                                                                                                                                                    >  1:978792/1‑227 (MQ=255)
                        agaAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGGTTTTTTTCCCCGCCCGttgtcccccccacgaaaaaa                                                                                                                                                                                                                   >  2:65732/1‑221 (MQ=255)
                         gaAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGata                                                                                                                                                                                                                  <  1:199258/236‑1 (MQ=255)
                         aaaaCGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGata                                                                                                                                                                                                                  <  1:65732/235‑1 (MQ=255)
                           aaCGCGCCACCAATCACCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGACCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTc                                                                                                                                                                                                             <  1:629115/239‑1 (MQ=255)
                           aaCGCCCCCCCAATCTACAACAGAGGCGTAACCGCCCACGGGATAAACGCACGGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTCCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTc                                                                                                                                                                                                             <  2:899975/239‑1 (MQ=255)
                             cgcgCCACCAATCATCAACAGAGGCGTAATCGGCCACGGGATAAACGCCCTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCa                                                                                                                                                                                                           <  1:623694/239‑1 (MQ=255)
                              ccccccccaATCCTCAAACGGGGCGTAATCGCCCCCGGGAAAACCGCACTGATGATCAGCGCCAGCGGCCCCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCag                                                                                                                                                                                                          <  2:467571/233‑1 (MQ=255)
                                ccccacaATCAACAAACGTGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCagta                                                                                                                                                                                                        <  1:239108/234‑1 (MQ=255)
                                  caccaATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCagtagt                                                                                                                                                                                                      <  2:171333/239‑1 (MQ=255)
                                    ccaACCATCACCGGGGGCGTAAACCCCCCCTGGATAAACGCCCTGAAGAGCAGCGCCAGCGGCACCAGAGTCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAgtgt                                                                                                                                                                                                    <  2:978792/239‑1 (MQ=255)
                                               cAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTTCTCGCCAGTTTTCCCCACACGGAGTAAATCTCCAGTAGGGTgggcgtatctc                                                                                                                                                                                         >  1:312049/1‑230 (MQ=255)
                                                  agGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTTGCCCCCCGGTTGCCCATCCCCGGAGAATCGTCCCGTAATGGTGCGGTATCCTCgg                                                                                                                                                                                      >  1:144947/1‑238 (MQ=255)
                                                  agGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTTTCCGCCAGTTTTCCCCCCACGCAGAGATCGTCCCCAGGGGTGGCGGTATCATCgg                                                                                                                                                                                      >  1:24949/1‑238 (MQ=255)
                                                       tAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTCGCCCCCCGGTTGCCCCTCACCGAGATATCGTCCCGTAGGGTGGCGATattatcccagca                                                                                                                                                                                  >  1:621661/1‑232 (MQ=255)
                                                        aaTCGCCCCCGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAAc                                                                                                                                                                                <  1:510411/238‑1 (MQ=255)
                                                         acccccccCGGGAAAACCGCACTGGGGACCAGCGCCAGCGGCCCCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGt                                                                                                                                                                              <  2:978011/235‑1 (MQ=255)
                                                         aacccccACGGGAAAAACCCCCTTAAGAACCGCCCCAGCGGCCCCAGAATCCCTTTATTAATCAGCGACCCTTTCGCCACGCCCCAGCCCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGt                                                                                                                                                                              <  2:604781/235‑1 (MQ=255)
                                                         aTCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGGTTTTTTTGCTCCCCGTTTGCCCCTCACCCACATATTATCCCGTCGTGTGGGGGTTTCATCGAGCACCg                                                                                                                                                                               >  2:621972/1‑238 (MQ=255)
                                                           cGCCTATGGGATAAACGCAGCTATGTTCCGGCCCCGCGGCAACAGAAGCCCCTTAACAGACAGCCTTCCTTTCGCCACGCCCCAGGCCACGGGAAGTTCCCGGTGGGCCCGCACGGCGGAACCTTGTTGCGCCTTGAGAGATAAGTCATCCCCTAATACACCGGCGGTTTGCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTATTGTGGCGATATCATCGAGCAACGTCa                                                                                                                                                                            <  2:730457/239‑1 (MQ=255)
                                                            cccccggggATAAACCCCCTGATGGTCCGCGCCGGCGGCACCAGAATCCCTTTATTAATCGGCGCGCCGTCGCCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAg                                                                                                                                                                           <  2:382792/233‑1 (MQ=255)
                                                                       aaccgcccggattatCAGCGCCAGCGGCACCAGAATCCCTTTATTAATCAGCGACCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTc                                                                                                                                                                 <  2:56581/229‑1 (MQ=255)
                                                                               ggtttacccgcgcCAGCGGCCCCAAAATCACTTTATTAATCAGCGATCCCTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCaaa                                                                                                                                                       <  2:807538/230‑1 (MQ=255)
                                                                                    atCAGCCCCCGCGGCACCATAACTCTTTTATTAATCCGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCAt                                                                                                                                                  <  1:355025/239‑1 (MQ=255)
                                                                                         cgcCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTTCCCCTCACCGGGATATCGTCCAGTCGTGTGGGGGGATCATCGAGCAACGTCCGTAAAAACCTCCCCGCCcaaaaaatttcc                                                                                                                                              >  1:847166/1‑230 (MQ=255)
                                                                                          gcCAGCGGCACCAGAATCACTTTATTAAGCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGGGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCct                                                                                                                                             <  1:1066159/238‑1 (MQ=255)
                                                                                          gcCAGCGGAACCAGAATCACTTTATTAATCAGCGATCATTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCct                                                                                                                                             <  1:621972/238‑1 (MQ=255)
                                                                                          ccccgCGGCACCAGAATTCTTTTATTAATCAGCGATCCTCTCGCCACGCCCCAGACCCCGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGAAAAGTCATCCCCGAATACACCGGCGGTTTTCTTCGCCGCCAGGTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTcc                                                                                                                                               <  2:277416/232‑1 (MQ=255)
                                                                                           ccAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTCGCCTCCAGATTTCCCATCACGGAGATATCGTCCAGTATTGTGGGCATATCATCGTGCAACGCCACGAAACTAATCCCCGCCCAAAGAAATCCatc                                                                                                                                            >  2:125614/1‑238 (MQ=255)
                                                                                                   cccaacaTCACGTTATTAATCACGGATCCGTTCGCCCACCCCCAGACCCCGGGAATTTCCCGGTTGGCCCGCACGCCTGAACCTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTa                                                                                                                                   <  2:844720/233‑1 (MQ=255)
                                                                                                   aCCAGAATCCCTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTa                                                                                                                                   <  1:745866/239‑1 (MQ=255)
                                                                                                          tcccTTTATTAATCACCGTTCCTTTCCCCACGCCCCAGACCCCGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCGAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGa                                                                                                                            <  2:504417/236‑1 (MQ=255)
                                                                                                           tccTTTTTTAATCCGCCATCCTTTCCCCACGCCCCAGACCCCGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  2:847166/237‑1 (MQ=255)
                                                                                                           ctcTTTTTTAATCAGCGGTCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  2:144947/237‑1 (MQ=255)
                                                                                                           ctcTTTTTTAACCCGCGTCCCTTTCCCCCCCCCCCAGACCCCGGGAAGTGCCCGGTTGGCCCGCACCCCTGAAACTTGTTGCGCATTGAGCCATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  2:117558/237‑1 (MQ=255)
                                                                                                           ccttttATTAACCAGGGACCCTTCCGCCCCGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  2:569806/236‑1 (MQ=255)
                                                                                                           cccTTTTTTAATCAGCGGTCCTTTCGCCCCGCCCCAGACCCCGGGAAGTTCCCGGTTGGCCCGCACGCCGGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  2:947390/237‑1 (MQ=255)
                                                                                                           cccTTTAATAACGAGCGATCCCTTCGCCACGCCCCAGACCCCGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATCCACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAACAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  1:1088704/237‑1 (MQ=255)
                                                                                                           ccatttATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  1:967185/236‑1 (MQ=255)
                                                                                                           cACTTTTTTAATCCGCGCTCCTTTCGCCCCGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                                                                           <  2:24949/239‑1 (MQ=255)
                                                                                                            aCTTTATTAATCAGGCATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAg                                                                                                                          <  1:1009095/239‑1 (MQ=255)
                                                                                                                  ttaATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTTTCCGCCCGTTTGCCCATCACGGAGATATCGCGCAGTAGTATGGCGCTATCATCGCGCAACACGCGTAAAATTATCCCCGCCAAAAAAAATCCCCCCCCCTTTTTTTTTTTGAAggggta                                                                                                                     >  2:373367/1‑236 (MQ=255)
                                                                                                                        aGCGATCCTTTCGCCAAGCCCTAGACCTCGGGAAGTTACCGGTTGGCCCGCACGCCTGAAACGTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCTGCGGTTTTCTTAGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCct                                                                                                                                             <  1:41727/208‑1 (MQ=255)
                                                                                                                                    cccccccccccaccccccggAAGTTCCCGTTTCGCCCCCCCGCCTGAAACTTGTTGCCAATTGAGCGATAAGTCATCCCCTAATCACCCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACgg                                                                                                  <  2:773085/221‑1 (MQ=255)
                                                                                                                                     cccccccccCGACCCCGGGAAGTTCCCTGTTGGCCCGCACGCCGTAAACGTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACCCCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGc                                                                                                 <  2:542246/234‑1 (MQ=255)
                                                                                                                                      caggCCCCAGACCACGGGAAGGTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACgg                                                                                                  <  1:38726/234‑1 (MQ=255)
                                                                                                                                         gCCCCAGGCCACGGGAAGTTCCCGGTTGCCCCGGATCCCTGAAACTTGGTGCGCATTGAGCGATAAGTCATCCCCTAATGCTCCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCTCGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGct                                                                                             <  1:280987/239‑1 (MQ=255)
                                                                                                                                                    cGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTCGGCGCCCGCTTGGCCATCCCCGAGGCATTGTCCAGTAGTGTGGCGGTATCATCCGGCAACCCCCACAAAACACTCCCCCCCACCAACATTTCCCCCCCTTTTTTTTTTTTTAGGGGGTAAGGATGAAGCAAAAACACCAGCACTgtcaaatt                                                                                   >  2:38726/1‑231 (MQ=255)
                                                                                                                                                     gggAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTCTCCGCCCGGTTGCCCATCCCCGGGATATCGTCCCCGAGGGTGGGGAGATCATCCCGCAAACCGCGGAAAACAATCCCCGCCCAAAGAATTCCCCTCCATTTTTTTTTTTTTAGGGGGTGAGGATAAAGAAAAAAAAGCAGCTCTGAttaatttt                                                                                 >  2:275357/1‑232 (MQ=255)
                                                                                                                                                         aGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATTCACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTca                                                                              <  1:837068/238‑1 (MQ=255)
                                                                                                                                                                           tggtcTGAACTTTGTTGCGCAATGAGCGTTAAGTCATCCCCTAATACCCCGGCGGTTTTCGTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTaa                                                           <  1:327615/235‑1 (MQ=255)
                                                                                                                                                                           aCGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTaa                                                           <  1:373367/239‑1 (MQ=255)
                                                                                                                                                                                tGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATc                                                      <  1:275357/239‑1 (MQ=255)
                                                                                                                                                                                   atcTTGTTGCCCATTGAGCGATAAGTCATCCCCTAATACCCCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAg                                                   <  1:341737/237‑1 (MQ=255)
                                                                                                                                                                                    aCTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCAtttt                                                                                                                                     <  1:685693/156‑1 (MQ=255)
                                                                                                                                                                                     ttttttgGGCATTGGGGGTTAAGTCACCCCCGAAAACCCCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAATGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTg                                                 <  2:493176/235‑1 (MQ=255)
                                                                                                                                                                                     ctgtttgCGCATTGGGGGATAAGTCTCCCCCAAAGACCCCGGCGTTTTTCTTCGCCGCCAGTTGGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTg                                                 <  2:783697/235‑1 (MQ=255)
                                                                                                                                                                                                          agtcccccccTAATTCCCCGGCGTTTTTTTTTGCCGCCCGTTTGCCCATCACGGAGATTACGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCcagca                            <  2:379924/233‑1 (MQ=255)
                                                                                                                                                                                                                  ccTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGgtgt                                                                                                                     <  1:266407/142‑1 (MQ=255)
                                                                                                                                                                                                                    tAATCCCCCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGAtttt                  <  2:772421/239‑1 (MQ=255)
                                                                                                                                                                                                                         cacCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGt             <  1:775924/239‑1 (MQ=255)
                                                                                                                                                                                                                          cccGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCCCGGAGAATTCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGtt            <  2:401108/238‑1 (MQ=255)
                                                                                                                                                                                                                           ccGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAg                                                   <  1:229069/199‑1 (MQ=255)
                                                                                                                                                                                                                            cggcggTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTa          <  1:125614/239‑1 (MQ=255)
                                                                                                                                                                                                                               ggggTTTTCTTTGGCGCCAGTTTGCCCATCCCGGAGATATCGTCCAGTAGGGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACt        <  2:621661/237‑1 (MQ=255)
                                                                                                                                                                                                                                     tttCCTCGCCGCCAGTTTGCCCATCCCGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGaa   <  1:96335/239‑1 (MQ=255)
                                                                                                                                                                                                                                      ttcttcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAac  <  1:332489/239‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              
ACTTTCTCTACTCCTTCAAAGCAGAGAAACGCGCCACCAATCATCAACAGAGGCGTAATCGCCCACGGGATAAACGCACTGATGATCAGCGCCAGCGGCACCAGAATCACTTTATTAATCAGCGATCCTTTCGCCACGCCCCAGACCACGGGAAGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCAATTATATCTCTCGCCAGCAGAATGATTTTCACGTTTACTATGAAC  >  NC_000913/2029044‑2029510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: