Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,459,579:1 +G 6.2% coding (889/1311 nt) fadI ← beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,459,5771.G 6.2% 258.0 / 11.9 64coding (891/1311 nt)fadIbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base . (31/29);  new base G (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

TCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCA  >  NC_000913/2459306‑2459859
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                          
tCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAc                                                                                                                                                                                                                                                                                       >  2:181202/1‑277 (MQ=255)
tCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAc                                                                                                                                                                                                                                                                                       <  1:181202/277‑1 (MQ=255)
tCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAAt                                                                                                                                                                                                                                                                             >  1:93263/1‑287 (MQ=255)
        ccAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGccc                                                                                                                                                                                                                                                                   <  2:273721/289‑1 (MQ=255)
          aaGCACGTTAAATTTGCTAGCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCGCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGAACAGGCTGGACCGAGCAACATGTCCCGCCAGACATCACTCGCAGGAAATGCGTAGCTGCGCAGATACCCCAGAGGCACCAGCCCTAATTATTTCGCCggg                                                                                                                                                                                                                                                                 >  2:259503/1‑289 (MQ=255)
              aCGTTAAAGTTGCTATCGTCCAGTTCGCCAGTGGCATGGGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGAtt                                                                                                                                                                                                                                                             <  1:291995/289‑1 (MQ=255)
                  tAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCCCCAGCCCTAATTCTTTCCCCCGGGATTCCGt                                                                                                                                                                                                                                                         >  1:494691/1‑289 (MQ=255)
                  tAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAcc                                                                                                                                                                                                                                                                                      >  1:71718/1‑260 (MQ=255)
                  tAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAcc                                                                                                                                                                                                                                                                                      <  2:71718/260‑1 (MQ=255)
                  tAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAg                                                                                                                                                                                                                                                          >  1:146226/1‑288 (MQ=255)
                     aTTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGCCATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGtca                                                                                                                                                                                                                                                       >  2:512140/1‑288 (MQ=255)
                                    cTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGc                                                                                                                                                                                                                                       <  2:146226/289‑1 (MQ=255)
                                                 aTGTGCACGCCCACGTGATTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGt                                                                                                                                                                                                                           <  2:490938/288‑1 (MQ=255)
                                                         gCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGt                                                                                                                                                                                                                  >  1:51250/1‑289 (MQ=255)
                                                                     cACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACCTGTCCTGCCAGACATCAATCGCCGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACAAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCCGGATCACCGCTGCCGCGCCATCGGCCAGCGCCGTACTGTTTgccg                                                                                                                                                                                                       >  1:283805/1‑288 (MQ=255)
                                                                     cACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTgcc                                                                                                                                                                                                        >  1:389595/1‑288 (MQ=255)
                                                                     cACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAcc                                                                                                                                                                                                                                                                                      <  2:25314/209‑1 (MQ=255)
                                                                     cACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAcc                                                                                                                                                                                                                                                                                      >  1:25314/1‑209 (MQ=255)
                                                                     cACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTgccg                                                                                                                                                                                                       >  1:164950/1‑288 (MQ=255)
                                                                     cACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCCCGCCATCGGTCAGCTGCGTACTGTTTgccg                                                                                                                                                                                                       >  1:507247/1‑288 (MQ=255)
                                                                     cACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCCTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCTGATACCCCAGCGGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTgcc                                                                                                                                                                                                        >  1:474710/1‑288 (MQ=255)
                                                                                               aaCTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTTCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGAt                                                                                                                                                                               <  2:47460/286‑1 (MQ=255)
                                                                                                         tCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGgcg                                                                                                                                                                  <  2:93263/289‑1 (MQ=255)
                                                                                                         tCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGgcg                                                                                                                                                                  <  2:51250/289‑1 (MQ=255)
                                                                                                           gCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCAAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCa                                                                                                                                                                >  2:239379/1‑289 (MQ=255)
                                                                                                              aGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCt                                                                                                                                                             >  1:522526/1‑289 (MQ=255)
                                                                                                              aGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCt                                                                                                                                                             >  1:85120/1‑289 (MQ=255)
                                                                                                                             aaGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCaa                                                                                                                                              >  1:99383/1‑289 (MQ=255)
                                                                                                                                   tCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAgg                                                                                                                                        >  2:290511/1‑289 (MQ=255)
                                                                                                                                     gTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAcc                                                                                                                                                                                                                                                                                      <  2:110900/145‑1 (MQ=255)
                                                                                                                                     gTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCAcc                                                                                                                                                                                                                                                                                      >  1:110900/1‑145 (MQ=255)
                                                                                                                                          tatCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTAcc                                                                                                                                 <  2:522526/289‑1 (MQ=255)
                                                                                                                                          tatCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTAcc                                                                                                                                 <  2:85120/289‑1 (MQ=255)
                                                                                                                                          tatCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTAcc                                                                                                                                 >  1:435435/1‑289 (MQ=255)
                                                                                                                                            tCGATCAATGTCAGATCGCTCATCTTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACcgc                                                                                                                               <  2:283805/289‑1 (MQ=255)
                                                                                                                                                      ccAGAGCGCGCAGCGTCAAACCGGGACGGTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATAttgt                                                                                                                      <  1:438573/287‑1 (MQ=255)
                                                                                                                                                              cTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGGCAGGAATTACCGCGAATTTTTTTTTCttgc                                                                                                              >  1:517450/1‑286 (MQ=255)
                                                                                                                                                              cTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGGGGAATTACCGCGACTATTGTTGTCTTCg                                                                                                              >  1:463527/1‑288 (MQ=255)
                                                                                                                                                                cATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACa                                                                                                           <  2:99383/289‑1 (MQ=255)
                                                                                                                                                                  tCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTCGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAg                                                                                                         <  2:278793/289‑1 (MQ=255)
                                                                                                                                                                   cGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTTTTTGACAAGc                                                                                                        >  2:432956/1‑289 (MQ=255)
                                                                                                                                                                      cAAACCGGCACGCGCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGAAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCgg                                                                                                      <  2:494691/288‑1 (MQ=255)
                                                                                                                                                                      aaaaCCGGCACGCCCCCGCGCCGGCGGTGTTTACCAGGCGGGACCGAGCAACAGGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGgt                                                                                                     <  1:424658/288‑1 (MQ=255)
                                                                                                                                                                        aaCCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGgttg                                                                                                   >  1:363262/1‑289 (MQ=255)
                                                                                                                                                                             gCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTtat                                                                                              >  1:85257/1‑289 (MQ=255)
                                                                                                                                                                              cACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTtat                                                                                              <  2:164950/288‑1 (MQ=255)
                                                                                                                                                                                 gcgccagcgccagcgGGGTTGAACAGGCTGGACCGAGCAACAGGTCCTGCCAGAAATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGAACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                                                                                          <  2:385150/286‑1 (MQ=255)
                                                                                                                                                                                 gCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                                                                                          <  1:133241/289‑1 (MQ=255)
                                                                                                                                                                                   tCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGGGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagga                                                                                         <  1:259503/288‑1 (MQ=255)
                                                                                                                                                                                               ggTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAgg                                                                               <  2:363262/286‑1 (MQ=255)
                                                                                                                                                                                                        cAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCAcc                                                                    >  2:5137/1‑288 (MQ=255)
                                                                                                                                                                                                           gCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACctct                                                                 >  2:276189/1‑288 (MQ=255)
                                                                                                                                                                                                                    aGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                                                                                          <  2:494096/254‑1 (MQ=255)
                                                                                                                                                                                                                    aGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAagg                                                                                          >  1:494096/1‑254 (MQ=255)
                                                                                                                                                                                                                    aGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATACGGAGGGATAAAGGCCGTCATCACCTCTTCTTTGAttt                                                       >  1:140113/1‑289 (MQ=255)
                                                                                                                                                                                                                                 gCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGAcaa                                          >  1:376532/1‑287 (MQ=255)
                                                                                                                                                                                                                                                cAGGAAATGCGTAGCTGGGCAGATACCCCAGCGGCACCGGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCGGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACg                           <  2:517450/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            aGCTGCGCTGATACCCCAGCGGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGaa                 <  2:474710/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            aGCTGCGCAGATACCCCAGCGGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGaa                 <  2:389595/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            aGCTGCGCAGATACCCCAGCGGCCCCAGCGCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACg               <  2:142779/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACgg              >  1:50041/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        aCCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACCCTGATGCGAACGGTGCGCTAATg    >  1:70395/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                         ccccAGCGGCACCGGCCCTAATCCTTTCGCCCGGGATTCGGTCATCAGGATCACCGCTGCCGCGCCACCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCa  <  1:5137/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ccccAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCa  <  2:435435/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                          
TCGAACCGCCAAGCACGTTAAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAGATCGCTCATCGTCAAACCGGCACGCTCCAGCGCCAGCGGTGTTGACCAGGCTGGACCGAGCAACATGTCCTGCCAGACATCAATCGCAGTAAATGCGTAGCTGCGCAGATACCCCAGCGGCACCAGCCCTAATTCTTTCGCCCGGGATTCAGTCATCAGGATCACCGCTGCCGCGCCATCGGTCAGCGGCGTACTGTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCA  >  NC_000913/2459306‑2459859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: