Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,242 C→T 6.8% intergenic (‑500/+158) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,2420CT 6.8% 161.8 / 2.3 57intergenic (‑500/+158)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base C (32/21);  new base T (2/2);  total (34/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAG  >  NC_000913/4325978‑4326529
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                
cGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTa                                                                                                                                                                                                                                                                          <  1:476196/289‑1 (MQ=38)
                 gggAATGAGTAGGCCTGCTAAGAAGGACAGGCGGCGCATCAGGGAGTCGGCACCGTGGCCGGATGCGGCGTAAACGCCTTAGCCGGCGTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGAAGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTACACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                                                                                                                                                                                                                                          <  1:178669/288‑1 (MQ=11)
                      tttGTGGGCCTGAGAAGACGGGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGAGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTGGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa                                                                                                                                                                                                                                                    <  1:504278/289‑1 (MQ=255)
                        tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATCTGTAGGCCTGATAAGACCCGCAAGGCGCGCATCAGGGCGTCGGCCCCGTTTCCGGACGTGGCTTACACCGCTTATCCCGCCTAAggcgc                                                                                                                                                                                                                                                  >  1:229401/1‑286 (MQ=11)
                                                   cGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAg                                                                                                                                                                                                                        >  2:108312/1‑288 (MQ=37)
                                                               tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCACTCGGCACGGTTTCCGGATGGGGCGTGACCGCCTCATCCGCACTACGGAGGGTGCGGGAATTTGTAGGCCTGAACAGACGCGCAAGCGtg                                                                                                                                                                                                           >  1:506326/1‑288 (MQ=11)
                                                               tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGt                                                                                                                                                                                                            <  1:288926/288‑1 (MQ=21)
                                                                       gTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGCATTTGTATGCCTGATAAGACGCGCAAGCGTCGCATCAgg                                                                                                                                                                                                   >  2:76888/1‑289 (MQ=25)
                                                                         tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGGTAAGACGCGCAAGCGTCGCATCAGGc                                                                                                                                                                                                  >  2:288926/1‑288 (MQ=25)
                                                                         tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACGGAGGGTGCGGGAATTTGTAGGCCTGATTAGAcgcccgcgcggcccatcctgc                                                                                                                                                                                                  >  1:409660/1‑270 (MQ=255)
                                                                                      ggaaaCGCCGTGTCCGGCCTAGGGAGGGTGCGGGAATTTGTAGGCCAGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGTGACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGtt                                                                                                                                                                                     <  2:318702/286‑1 (MQ=255)
                                                                                               ttATCCGGCCTACGGATGGTGCGGGAATTGGTAGGCCTGATAAGACGCGCAAGCGTCGAATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATgcg                                                                                                                                                                           <  2:295175/289‑1 (MQ=255)
                                                                                                                    cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGc                                                                                                                                                       <  1:108312/288‑1 (MQ=255)
                                                                                                                      ggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGCCGCGCAAGCGTCCCATCAGGCATCAGGCCCCGTTGTCGGACGCGGCGCGAGCGCCTTATCCGGCtt                                                                                                                                                     >  1:29656/1‑288 (MQ=255)
                                                                                                                           ttGTATGCCTGTGAAGACGTGAAAGCGTGGCATCAGGAGGTTGGGACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGATGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGGACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGAACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGAt                                                                                                                                               <  2:181700/289‑1 (MQ=14)
                                                                                                                           ttGTAGTCCTGATAAGACGCGCAAGCGTCGCATCATGCAGTTGGCACTGTTGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGAt                                                                                                                                               <  2:427960/289‑1 (MQ=255)
                                                                                                                           ttGTAGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTAGCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGAt                                                                                                                                               <  2:17715/289‑1 (MQ=255)
                                                                                                                           tggTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGAt                                                                                                                                               <  2:75070/287‑1 (MQ=255)
                                                                                                                            tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTCGGCCTGATAAGACGCGCAAGCGGCGCATTAGGCATCTGGCACCGTTGCCGGATGCGGCGCGGGCGCCTTATCCGCCTTACGGag                                                                                                                                               >  1:427960/1‑287 (MQ=255)
                                                                                                                             ggcggCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGACGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATc                                                                                                                                              <  2:116646/285‑1 (MQ=255)
                                                                                                                                 gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTa                                                                                                                                                                                                                                                                          <  1:237660/160‑1 (MQ=35)
                                                                                                                                   cTGATAAGACGCGCAAGCGTCGCATCAGGCAGCTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCCTCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTAt                                                                                                                                                            >  1:424707/1‑268 (MQ=255)
                                                                                                                                   cTGATAAGACGCGCAAGCGTCGCATCAGGCAGCTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTAt                                                                                                                                                            <  2:424707/268‑1 (MQ=255)
                                                                                                                                    tGATAAGACGCGCAAGCGGCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCGTAGCCGGCCTACGGGGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCacaataca                                                                                                                                      <  2:17889/289‑1 (MQ=255)
                                                                                                                                     gATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCCCCGTTGCCGGATGCGGCGTCAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATTTGTACGCCTGATAAGCAGCTCACGCGTCCCCACAGGCATCTGGCACCCTTGCCTGCTGCGCCCCGAGCCCCTTATCCGGGCTTCCGCTCacaatccac                                                                                                                                     >  1:385598/1‑288 (MQ=14)
                                                                                                                                      aTAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGtt                                                                                                                                                                                     <  1:226041/240‑1 (MQ=38)
                                                                                                                                      aTAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGtt                                                                                                                                                                                     >  2:226041/1‑240 (MQ=38)
                                                                                                                                           aCGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACCATACAATCAGt                                                                                                                                >  1:217833/1‑288 (MQ=255)
                                                                                                                                                         cATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGTTACCGATc                                                                                                                                              <  2:64029/259‑1 (MQ=255)
                                                                                                                                                         cATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGTTACCGATc                                                                                                                                              >  1:64029/1‑259 (MQ=255)
                                                                                                                                                              ggCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGCGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGTCACCCTTTCCGGCTGCGGCTCGATCCCCTTATCCGGCTTACCGATCACAATACACTCAGTTCTTTCTCACTAACTCatct                                                                                                            >  1:305378/1‑286 (MQ=17)
                                                                                                                                                                   ttGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCCCCGTTGCCGGATCCGGCGCGAGCGCCTTATCCGGCTTTCCCATCACTATACTATCAGTTTTTTTTTCCATACTCAGAATTc                                                                                                         >  1:222264/1‑287 (MQ=14)
                                                                                                                                                                           gTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGc                                                                                                                                                                                                                                >  1:84970/1‑160 (MQ=14)
                                                                                                                                                                             tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGCTAAGACGCGCACGCGTCGCTTCAGGCATCTGGCCCCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGATTACCGATCACACTACACTCAGTTCTTTTTCACCAACTTAGCTTTCAGTTTCCTCgta                                                                                            >  1:490959/1‑287 (MQ=17)
                                                                                                                                                                                                   tgagccGGCCTACGGGGGGTGCGGGAAGTGGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAAt                                                                      <  2:229401/285‑1 (MQ=255)
                                                                                                                                                                                                           ccTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTACCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTa                                                              >  1:110146/1‑289 (MQ=255)
                                                                                                                                                                                                               cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGGGGGAATTTGTAGGCCTGAAAAGACGCGCAAGCGTCGCAACAGGCATCTGGCCCCGTTGCCGGATGCGGGCGGGGCGCCTTATCCGGCTTCCCGATCACAATCATAACAGTTCTTTTTCCCAAACTCAAATTTCAGTTTCATCCGCACCAAAACGTCCATTTTGCAATCgcattttaggt                                                          >  1:456931/1‑279 (MQ=17)
                                                                                                                                                                                                                 gAGGGTGCTGTGATGTGTAGTACTGATAAGACGCCCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGt                                                          <  2:506326/287‑1 (MQ=255)
                                                                                                                                                                                                                    ggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCACCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCCCAATACAATCAGTTGTTTTTCACAAAGTCAGATTTCAGTTTCATCTGACCAAAACCGTCGCTTTTGCAATCGATGTGATGGTGGCCt                                                     >  1:331347/1‑289 (MQ=255)
                                                                                                                                                                                                                         gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACCATACAATCAGTTCTTTTTCACAAACTCAGCTTTCAGTTTCATCGGACCAAACCCTGCGATTTTGCAATCGATGTTATGGTCGCCTTCaa                                                 >  2:74983/1‑288 (MQ=255)
                                                                                                                                                                                                                         gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGCCCAAAACCGTCGAATTTGCAATCGATGTTATGGTCGCCTTCaa                                                 >  2:155057/1‑288 (MQ=255)
                                                                                                                                                                                                                         gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCaa                                                 >  2:436885/1‑288 (MQ=255)
                                                                                                                                                                                                                            aaTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGt                                                                                                                                                                                                            <  1:151250/131‑1 (MQ=32)
                                                                                                                                                                                                                                     gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTAcc                                                                                                                                                  >  1:391846/1‑180 (MQ=255)
                                                                                                                                                                                                                                     gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTAcc                                                                                                                                                  <  2:391846/180‑1 (MQ=255)
                                                                                                                                                                                                                                     gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGCTCACAATACAATCAGTTCTTTTTCACAAAATCCGATTTCAGTTTCATCGGACCAAAACCGACGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGCTGtt                                    >  2:354787/1‑289 (MQ=255)
                                                                                                                                                                                                                                     gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAAACAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCCATCGAATTTATGGTCGCCTTCAACCAGGCGGATGtt                                    >  1:95222/1‑289 (MQ=255)
                                                                                                                                                                                                                                      ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGtt                                    <  2:29656/288‑1 (MQ=255)
                                                                                                                                                                                                                                         gATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTc                                >  2:87035/1‑289 (MQ=255)
                                                                                                                                                                                                                                          aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCCGGCATCTGGCACCGTTGCCGGATGCGGCGCGCGCGCCTTATCCGGCTTACCGCTCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCCGTTTCATCGTACCAAAACCGGCGATTTTGCAATCGTTCTCAGGGTCCCCTTCAACACGTCGGATGtttttt                                >  1:293491/1‑287 (MQ=21)
                                                                                                                                                                                                                                               aCGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTTCTATTGATGTTATGGTCGCCTTCCACCCGGCGGATGTTTTTACttttg                         >  1:218263/1‑287 (MQ=255)
                                                                                                                                                                                                                                               aCGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTg                          >  1:265327/1‑289 (MQ=255)
                                                                                                                                                                                                                                               aCGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTg                          >  1:333244/1‑289 (MQ=255)
                                                                                                                                                                                                                                                 gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGt                        >  2:189276/1‑289 (MQ=255)
                                                                                                                                                                                                                                                  cgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCCGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGt                        >  1:128121/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    cAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGcc                     >  2:252411/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cAGTTGGCACTGTTGCCGGATGCGGCGGAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCg       <  2:456931/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAagag  <  1:436885/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                
CGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTATCCGGCTTACCGATCACAATACAATCAGTTCTTTTTCACAAACTCAGATTTCAGTTTCATCGGACCAAAACCGTCGATTTTGCAATCGATGTTATGGTCGCCTTCAACCAGGCGGATGTTTTTCACTTTGGTGCCAATTTTCAGCATCGAAGAG  >  NC_000913/4325978‑4326529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: