Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,625,882 | T→G | 5.7% | intergenic (+18/‑31) | serB → / → radA | 3‑phosphoserine phosphatase/DNA repair protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,625,882 | 0 | T | G | 5.7% | 236.0 / 4.7 | 70 | intergenic (+18/‑31) | serB/radA | 3‑phosphoserine phosphatase/DNA repair protein |
Reads supporting (aligned to +/- strand): ref base T (32/33); new base G (2/3); total (34/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 |
TTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTT > NC_000913/4625599‑4626163 | ttACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTgcggg > 1:121247‑M2/1‑284 (MQ=255) ggCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca < 2:276615/289‑1 (MQ=255) tgtgATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCACCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGcaaacca > 1:499514/1‑284 (MQ=255) gtgATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCAt < 1:46312/289‑1 (MQ=255) gttCGCCGACGTCGTTGACCCGCAGTACAAAGCGAAAACCCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTa < 1:87204/287‑1 (MQ=255) cATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTac > 1:155267/1‑289 (MQ=255) gTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGc > 2:326382/1‑288 (MQ=255) gAAAACGCTGACTCGCCTGGCGCAGGATCATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGCGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAAcg < 1:270536/288‑1 (MQ=255) aaaCTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGggcggcc > 1:307978/1‑245 (MQ=255) aaaCTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca < 2:307978/246‑1 (MQ=255) aaaCTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAAcgcg > 1:24806/1‑288 (MQ=255) aaaCTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAAcgcg > 2:228229/1‑288 (MQ=255) aaaCTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAAcgcg > 1:198682/1‑288 (MQ=255) gACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCtttgtt < 2:155267/289‑1 (MQ=255) ctcgcctcgcGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCtttgttt < 2:137071/288‑1 (MQ=255) cgcggcgcgcgGAGGAGTATGAAATCCCGCTGGCGCAGACCGGGGCGATTGGCGATGGAGCCAATGAACTGCCGATGATCAAAGCGGCAGGGCTGGGGAGTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCtttgttt < 2:108898/281‑1 (MQ=255) aaTCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTAt < 2:24806/289‑1 (MQ=255) tCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATcc > 2:266260/1‑289 (MQ=255) cccGCTGGCGCGGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCcg < 1:337212/289‑1 (MQ=255) cccGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCcg < 1:73235/289‑1 (MQ=255) cGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCcgc > 2:412391/1‑288 (MQ=255) cgcAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGc < 1:326382/288‑1 (MQ=255) ggagaCCGTGGCGATTGGCGAGAGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCAGGATGgcgggcgagcaattacttctgattcaggctgcctgagaggatgcagaatacccccatcaggtcagcgtgacggatggtgacttccgccttttcat > 1:500131‑M2/3‑193 (MQ=255) ccgggggcgattggcgatGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAgggg < 1:159020/283‑1 (MQ=255) ccGTGGCGATGGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAgggg < 2:201853/287‑1 (MQ=255) ggcagttggcgagGGAGCCAATTACCTGCCGATGATCAAAGGGGCAGGGGTGGGGATTGCCTACCAAGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCCCCATCCGTCACGCTGACCTGATGGCGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGCGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGgcagtg < 2:499514/283‑1 (MQ=255) ttggcgatGGAGCCAATGACCTGCGGAGGATCAAGGCAGCAGGGCTTGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGGTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCagtgcagtgc < 2:288392/289‑1 (MQ=255) aCCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAacac < 2:198682/289‑1 (MQ=255) gCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATc > 2:43672/1‑289 (MQ=255) cGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCAc < 1:442233/289‑1 (MQ=255) gatCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAg > 2:193646/1‑289 (MQ=255) aaGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGTACCCCCACACCGAggg > 1:53540/1‑285 (MQ=255) aaGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCg > 2:290934/1‑289 (MQ=255) cAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTtg > 1:144771/1‑288 (MQ=255) cAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCAACGAGGTGCGTCtt > 1:44415/1‑287 (MQ=255) cTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCg < 1:228229/288‑1 (MQ=255) cTGGGGATTGCCTACCATGCCAAGCCAAAAGGGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCg < 1:78713/288‑1 (MQ=255) tGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAAtg > 2:357197/1‑289 (MQ=255) gCCTACTATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAAtgg > 2:351956/1‑289 (MQ=255) gCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGGCGCCCAtgg > 2:393510/1‑289 (MQ=255) cTACCATGGCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCGGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAAtggt < 1:298169/288‑1 (MQ=255) tACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAAtggtgg < 1:43672/289‑1 (MQ=255) aTGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGcgcg > 1:480149/1‑289 (MQ=255) ccaagccaaAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAAGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCCCCGTGGTGCGTCTTGCTGCCTCGCCAATGGTTGCGCGTac > 1:345508/1‑288 (MQ=255) aatgaaAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGGGCCTGTCATGCCTGGAACACCATCACCGAGGTCCGTCTCGCTGCGTCGCCAATGGTGGCGCGTAACGCGCGGCTCAGCg > 2:15115/1‑289 (MQ=255) aCCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCg > 1:403027/1‑289 (MQ=255) tCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCa > 1:311826/1‑289 (MQ=255) tGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca > 1:149606/1‑80 (MQ=255) tGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGca < 2:149606/80‑1 (MQ=255) aCCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCa > 2:314958/1‑289 (MQ=255) aTGGGGGTAATGTGCATCCTGTCAGGCAGCCTAGATCAGAAGTAATTGCCCGCACTCCATCCTTGGGGCGGCACAGCATTAACGGGGTACACGGTGGCAAAAGCTCCAGAACGCGCCTTGTTTTGGAATGAGTGCGGGGCCGATTATCCGCGCTGGCAGGGGAAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGaaa < 1:351956/288‑1 (MQ=255) gggTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAActct < 1:266260/288‑1 (MQ=255) ggTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCt > 2:479749/1‑113 (MQ=255) ggTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCt < 1:479749/113‑1 (MQ=255) tCTGCATGCCCTCAGGCAGCCGGAATCAGAAGTAATTGTTGGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGatat < 1:197007/289‑1 (MQ=255) tCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGatat < 1:193646/289‑1 (MQ=255) tCTGCATCCTCGCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGatat < 2:53540/289‑1 (MQ=255) cATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCAGGATGgctggcgagcaattacttctgattcaggctgcctgagaggatgcagaatacccccatcaggtcagcgtgacggatggtgacttccgccttttcattcacttttggcttggcatggtaggcaatccc > 2:28392‑M2/1‑49 (MQ=255) cATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCAGGATGgcgggcgagcaattacttctgattcaggctgcctgagaggatgcagaatacccccatcaggtcagcgtgacggatggtgacttccgccttttcattcacttttggcttggcatggtaggcaatccc < 1:28392‑M2/175‑127 (MQ=255) aTCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGcc < 2:403027/289‑1 (MQ=255) cAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTGCAGAAAATCCCCGATATCAGCCTTGAaga > 2:284532/1‑289 (MQ=255) ccTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAgggg < 2:422675/133‑1 (MQ=255) ccTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAgggg > 1:422675/1‑133 (MQ=255) ccTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGcc > 2:374100/1‑288 (MQ=255) aaGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCGGGGGTGGCAAACGTACAGAAACTCACCGATATCAGCCTTGAAGAGCGGCCGCGTTTTTcc > 1:14627/1‑288 (MQ=255) aGTAATTGCTGGCCCGCCATCCGGCGGGCGGAACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCAc < 1:162262/289‑1 (MQ=255) aGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCAc < 1:357197/289‑1 (MQ=255) aGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCAc < 2:44415/289‑1 (MQ=255) cccgccATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCCCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTGGAACAGCGGCCGCGTGTTTCTCCCGGACTTAAaga > 1:301519/1‑289 (MQ=255) gccATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGtt < 2:144771/289‑1 (MQ=255) | TTACCGGCAATGTGATCGGCGACATCGTAGACGCGCAGTACAAAGCGAAAACTCTGACTCGCCTCGCGCAGGAGTATGAAATCCCGCTGGCGCAGACCGTGGCGATTGGCGATGGAGCCAATGACCTGCCGATGATCAAAGCGGCAGGGCTGGGGATTGCCTACCATGCCAAGCCAAAAGTGAATGAAAAGGCGGAAGTCACCATCCGTCACGCTGACCTGATGGGGGTATTCTGCATCCTCTCAGGCAGCCTGAATCAGAAGTAATTGCTCGCCCGCCATCCTGCGGGCGGCACAGCATTAACGAGGTACACCGTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGCCTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCAATGGTGGCGCGTAACGAGCGTCTCAGCGGCTATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGATTTAAAGAGTT > NC_000913/4625599‑4626163 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |