Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 129.2 / NA 47I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/25);  total (22/25)

GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132654‑2132922
                                                                                                                                     |                                                                                                                                       
gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                >  1:1096736/1‑143 (MQ=255)
           aCAGCTGAGATTTTTTGCCGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt                                                                                                                     <  1:335658/143‑1 (MQ=255)
                tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                >  1:247903/1‑143 (MQ=255)
                 gATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCAc                                                                                                               >  1:541587/1‑143 (MQ=255)
                  atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                              <  1:766736/143‑1 (MQ=255)
                  atatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                              >  2:454947/1‑143 (MQ=255)
                       ttttGCTCGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGACTTTCTTCGGTGTCTATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCg                                                                                                         <  1:447953/143‑1 (MQ=255)
                          tGCTGGCGCGGTCTCATTTGTCTTTGTCCTTGCGGTACACGTCCAAGTACACGTAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                      <  1:679462/143‑1 (MQ=255)
                             tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                   >  1:80087/1‑143 (MQ=255)
                                          tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCCTACTCCACCCCCAGcc                                                                                      >  2:171744/1‑143 (MQ=255)
                                              tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                  >  1:854798/1‑143 (MQ=255)
                                              tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                  <  1:570958/143‑1 (MQ=255)
                                                tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTGCACGGTTCGCCGTAATCCACCACCATCCGCCggc                                                                                >  2:870952/1‑142 (MQ=255)
                                                tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGa                                                                                >  1:1032957/1‑143 (MQ=255)
                                                 gtgtgCTGGCGGGAAACGGGGAAGTACCCGGAGAATTTTTTCGGTGACGTGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                               <  2:936109/142‑1 (MQ=255)
                                                       ttacGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                         <  1:751864/140‑1 (MQ=255)
                                                           ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTaa                                                                     >  1:776303/1‑143 (MQ=255)
                                                           ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAACAACAGCAAGAAAATGTCATaa                                                                     >  2:960789/1‑143 (MQ=255)
                                                             tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTAATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCATCGACCACCATCCACAGGAAGACAGCGTAATc                                                                   >  2:1042043/1‑143 (MQ=255)
                                                              aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGACGTACTCCACCGCCATCCGCAGGAAGATGTCGTAATcc                                                                  <  2:660145/143‑1 (MQ=255)
                                                               aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTTTTCCt                                                                 >  2:126621/1‑143 (MQ=255)
                                                                 aggggAAGTGCGCGGGGAAGTTTGTGGGTGACGGGGTGATCTGCAGTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                               <  2:698884/140‑1 (MQ=255)
                                                                 aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                               >  1:785903/1‑143 (MQ=255)
                                                                  gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                              >  1:917232/1‑143 (MQ=255)
                                                                             cGGCGAATTTTGTCGGTGACGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                   <  2:529138/143‑1 (MQ=255)
                                                                             cGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTTCAGt                                                   >  1:572873/1‑143 (MQ=255)
                                                                                    ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGCCTCCGCGTCGCCTCTTCTACTTTCCACGGTTCGCCACACTACCCCACCATCCACAGGCAGATGTCGTAATCCTGTGCCGCCTCCAggccgctat                                            >  2:12083/1‑135 (MQ=255)
                                                                                         tCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaac                                       <  1:197966/143‑1 (MQ=255)
                                                                                           ggTGGCGCGGTGATCTGCATTGCACCGCGATTGAGGTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                     <  2:755784/143‑1 (MQ=255)
                                                                                                 gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                               <  1:27944/143‑1 (MQ=255)
                                                                                                 gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCTTCAGTTCGGCACCGAACAAacaccc                               >  2:846886/1‑141 (MQ=255)
                                                                                                    gggATGTGCATGTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGGCGCTTTCAGTTCGGTATCGAACAAACACTCttt                            <  2:121827/141‑1 (MQ=255)
                                                                                                      gATCTGCGTTGCTCTGCGATTGATGTGCAGGCTGTGCGTGGCGCCTTCTACTTTCCAGGGTTCGCCGGCCTCCACCACCTGCCGCGGGACGATGTCGTAGTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                          <  2:527603/143‑1 (MQ=255)
                                                                                                      gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                          <  2:803793/143‑1 (MQ=255)
                                                                                                           gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAGAAACACTCTTTGAAACGc                     >  1:375316/1‑143 (MQ=255)
                                                                                                            cATTTCACCGTGATTGATCGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc                    <  2:630430/143‑1 (MQ=255)
                                                                                                             ttttCCCCGTGATTGATCGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCTCGGTTCGCCGTACTCCACCTCCATCCGCTGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                   <  2:50361/142‑1 (MQ=255)
                                                                                                             atgtctccGTGAGTGGTATGGAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                   <  2:633880/137‑1 (MQ=255)
                                                                                                             aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCa                   >  1:506810/1‑143 (MQ=255)
                                                                                                              ttgcACCGTGGTGGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCGTTCTCCACCTCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt                  <  2:452136/140‑1 (MQ=255)
                                                                                                                tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTGAAACGCCATGc                >  1:154020/1‑143 (MQ=255)
                                                                                                                    cGTGATTGATATGCAGGCTGTGCGTCGCCGCTTCGACTTTCCAGGGTCCGCCGTGCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg            <  2:825136/143‑1 (MQ=255)
                                                                                                                    cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGCTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTACAGCTCGGTATCGAACAGACACGCTTTGAAACGGCATGccgcg            >  2:737415/1‑140 (MQ=255)
                                                                                                                        attgatCTGCAGGCTCTGCGTCGCCTCTCCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaa        <  1:285940/143‑1 (MQ=255)
                                                                                                                            atctGCAGGCTGTGCGCCCCCTCTTCCGCTTTCCACTGTTCGCCGTACTGCAACACCATCCGCAGGAAGATGTCGTATTTCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTTTTTGAAACGCTATGCCCAGGTAAAGAc    <  2:959251/140‑1 (MQ=255)
                                                                                                                             tgtGCAGGCTTTGCGTCGCCGCGTCTACTTTCCGCGGTTCGCCGTACGCCACCACCAGCCGCAGGAAGATGGCGTAATCCGGGGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAcc   <  2:539453/141‑1 (MQ=255)
                                                                                                                              atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt  <  1:241650/143‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCT  >  NC_000913/2132654‑2132922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: