Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,542 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5420CA97.2% 207.8 / NA 74P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (34/36);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4184402‑4184682
                                                                                                                                             |                                                                                                                                            
tCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGc                                                                                                                                            >  2:470470/1‑143 (MQ=255)
      ggTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGCAAACCGCACGCCGGTAGCCATCGTACTGAACCAGCACGGCg                                                                                                                                      >  1:595790/1‑143 (MQ=255)
      ggTTAAACGCCGTATCCAGCCAGGAGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAAAGCAACCCTTTCGAAGATATGCCTTACGATGAAAACGGTACGCCAGTAGACATCGTACTGAACCAGCTGGGCg                                                                                                                                      >  1:938080/1‑143 (MQ=255)
         tAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTAc                                                                                                                                   >  1:9301/1‑143 (MQ=255)
            aCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGt                                                                                                                                >  1:131308/1‑143 (MQ=255)
               ccgtaccagtccTGGTGAAAAGATGGCAGGACGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAACACACTCTTTCAGTTGGGCGTACCGtctc                                                                                                                             <  1:706837/133‑1 (MQ=255)
               ccGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGtctc                                                                                                                             >  2:576751/1‑143 (MQ=255)
                cGTAGCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCg                                                                                                                            <  2:376254/143‑1 (MQ=255)
                  tagccAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGAGATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                          <  1:331687/140‑1 (MQ=255)
                   aactagcgtcgagACAAGATGGCAGCTGGTCACGGTAACAATGGTGTACTTTCTAAGATCAACCCGATAGAAGATATGCCTCACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAt                                                                                                                         <  1:797356/131‑1 (MQ=255)
                    tCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATg                                                                                                                        <  2:1044239/143‑1 (MQ=255)
                       aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACACCGTACTGAACCAGCGGGGCGTACCGTCTCGTATGAAc                                                                                                                     >  1:536742/1‑143 (MQ=255)
                          cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCGGATCGAAGATATGGCTTAAGATGAAAACGGTACGGCGGTAGACAGCGTACTGAAGCAGGTGGGCGTAACGTCGCGTAttagaatg                                                                                                                  >  2:92328/1‑136 (MQ=255)
                          cTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACAGCGTACTGAACCAGGTGGGCGTACCGTCTGGTATGAACAtt                                                                                                                  >  2:92329/1‑142 (MQ=255)
                           tGGTGACTATATGGCAGGTCGTCACGGTAACAAGTGTGTAATTTCTAAGATCATCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCg                                                                                                                 <  1:387283/143‑1 (MQ=255)
                           tGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGGTGACCCCGATCGAAGATATGCCTTACGAAGAAAACGGTACGCCGGTACACACCGGACTGGACCAGGTGGGCGTGCCGTCTCGCATGAACAACg                                                                                                                 >  1:933357/1‑143 (MQ=255)
                             gTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGt                                                                                                               >  1:997656/1‑143 (MQ=255)
                              tGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                              <  2:755969/143‑1 (MQ=255)
                              ggACAAGATGGGAGGTCGTTATGGGAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGGACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTc                                                                                                              <  2:605831/142‑1 (MQ=255)
                                   aGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc                                                                                                         <  1:474876/143‑1 (MQ=255)
                                   aGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATc                                                                                                         <  2:1001914/143‑1 (MQ=255)
                                     aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACAGTAGTCCGGACGAACCTCTCTTGAACCAGCTGGGCGTAACGGCTCGTAGGAACATAggggcgatccc                                                                                                       >  1:243290/1‑134 (MQ=255)
                                      tGGCAGGTCGTCACGGTATCAAGGGTGTAAGTTCTAAGATCCACCCGATCGACGGTTAACCATGGCTTTTAAACGGTATGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                      <  2:844923/143‑1 (MQ=255)
                                      tGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTc                                                                                                      <  2:9301/143‑1 (MQ=255)
                                       ggCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCg                                                                                                     >  1:1153457/1‑143 (MQ=255)
                                          gggTCGTCACGGTAACTACGGTGTAATTTCTCAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACTGTACGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                                  <  1:57033/142‑1 (MQ=255)
                                           ggTCGTCACGGGAACAAGGTTGGAGTTTTTAAGAGCAGCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGGCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAc                                                                                                 <  1:973697/143‑1 (MQ=255)
                                            gtcgtcAGGGTAACAAGGGTGTAATTTCTAAGATCAACCCTGTCGAAGATATGCCTTACGAAGGCACAATAACACCTTTAGGAATATTCGCTCAGTGGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAAcc                                                                                                <  2:1154405/143‑1 (MQ=255)
                                              cgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCa                                                                                              <  1:159950/143‑1 (MQ=255)
                                               gtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCTCGAAACCCAc                                                                                             >  1:1098282/1‑143 (MQ=255)
                                                       aaCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                     <  1:804091/143‑1 (MQ=255)
                                                       aaCAAGGGTGTAATTTCCACCATCAACCCGATGGAAGATATGCCTTACGATTAAAACGGCACGCCGGTACACATCGTACTGACCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                     <  2:94098/143‑1 (MQ=255)
                                                       aaCAAGGCCGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                                     <  2:500191/143‑1 (MQ=255)
                                                        aCAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATACTCGAAACCGACCTTGGTATg                                                                                    >  1:922168/1‑143 (MQ=255)
                                                        aCAAGGGTGTAATTACTAAGAGCCACCCGATCGCTGTTAAAATGAGCTACGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATg                                                                                    <  2:259190/143‑1 (MQ=255)
                                                         cAAGGGTGTAATTTCTAAGATCAACGCGATCGAAGATATGCCTTCCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAGACCCACCTGGGTATgg                                                                                   >  2:1041590/1‑143 (MQ=255)
                                                          aaGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTAGGTCTGGc                                                                                  >  1:799643/1‑143 (MQ=255)
                                                             ggagtACTTTCTAATACCAACCCGATCGAAGATATGCCTTACGATGAACACGGTACGCCGGTAGACATCGTACTGATCCCGCTGGTCGTACCGTCTCGTATGAACATCGGTCAGATTCTCGAAACCCACCTGGGTATGGCTGc                                                                               <  1:172240/140‑1 (MQ=255)
                                                               tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                             >  1:410846/1‑143 (MQ=255)
                                                                gtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaa                                                                            >  2:12259/1‑143 (MQ=255)
                                                                 tAATTTCTTATATCATCCCGATCTATGATATGCCTTACGACGAATACGTTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTTGAAACCCACCTGGGTATGGCTGCGaaa                                                                           <  1:622452/143‑1 (MQ=255)
                                                                 tAATTTCCAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCACCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                           <  2:809448/143‑1 (MQ=255)
                                                                 tAATCTCTAAGATCACCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                           <  2:823168/143‑1 (MQ=255)
                                                                  aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                          <  2:660268/143‑1 (MQ=255)
                                                                  aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGAATGGCTGCGAAAg                                                                          >  1:847546/1‑143 (MQ=255)
                                                                         aaGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACATGGGTGTGCCTGGCAAAGGGATCgg                                                                   >  2:493567/1‑143 (MQ=255)
                                                                           gATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCCCCTGGGTGTGGCTGCGAAAGGTCTCGGCg                                                                 >  1:537834/1‑143 (MQ=255)
                                                                            cccAACCCCATCGAAGAGATGCATTACCATGAAAACGGTACGCCGGTAGACATCGTACTGAACGCGCTGGGCATACCGTCTCGTATGCACATCGGTAAGATCCTCGCCCCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                                <  1:494901/141‑1 (MQ=255)
                                                                            aTCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGa                                                                >  2:901295/1‑143 (MQ=255)
                                                                              caccccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACa                                                              <  2:733699/140‑1 (MQ=255)
                                                                                aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                            <  2:1065600/143‑1 (MQ=255)
                                                                                aCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAg                                                            <  1:870772/143‑1 (MQ=255)
                                                                                 cccGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGa                                                           <  1:384199/143‑1 (MQ=255)
                                                                                  ccGATCGAAGATATGCCTTGCGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                          <  1:913973/143‑1 (MQ=255)
                                                                                      tctaaGATTTGCCTTACGATGAATATGGTATGTTGGTAGACATCGTACTGAACCAGCTGGGTGTACCGTCTCGTATGAACATTGGTCAGATCCTCTAAACCCACCTGGGTATGGCTGTGAAAGGTATCGGCGACAAGATCAAc                                                      <  1:52220/140‑1 (MQ=255)
                                                                                      tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                                      <  2:1056878/143‑1 (MQ=255)
                                                                                           gagatGCCTGACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTAGGAACAGCGGTCAGAGCCTCGAAACCCACCTGGGGATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCAt                                                 <  1:457155/140‑1 (MQ=255)
                                                                                                  ctcccGATGACACCGGCCCGCCGGCAGACATCGTACTGCCCCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCATATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                          <  2:9673/139‑1 (MQ=255)
                                                                                                  cTTACGATGAACACGGTACGCCGGTAGCCACCCTACCGCAGCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                          <  1:621456/143‑1 (MQ=255)
                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                          >  2:1125577/1‑143 (MQ=255)
                                                                                                  cTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGaaa                                          >  2:1107160/1‑143 (MQ=255)
                                                                                                   ttACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCAGCGCCCACATCAACGcagtggtgggac                                         >  1:393596/1‑132 (MQ=255)
                                                                                                    tACGATGAAAACGGTACTCCGGTAGACAGCGTACTGAACCAGCGGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAca                                        <  1:632714/143‑1 (MQ=255)
                                                                                                      cGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGCTCAGATCCTCGAAACCCACCTGGGTATGGCTGAGACAGGTGTCGGCGACGAGATCGAGGCCCGGCTGAAAcacc                                      >  1:923043/1‑141 (MQ=255)
                                                                                                       gATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagca                                     >  1:390272/1‑143 (MQ=255)
                                                                                                        aTGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAcagcag                                    >  2:337416/1‑143 (MQ=255)
                                                                                                           aaaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGCAAGGTATCGGCGACAAGATCAACGCCATGCTGAAAACgcagcac                                 >  1:852426/1‑142 (MQ=255)
                                                                                                            aaaCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACATCGGTCAGATCCCCGGAACCCCCCCGGGTATGGCTGCGACAGGTATCGGCGGCAAGATCGAGGCCATGCAGACACACGAGCaag                                >  1:452209/1‑143 (MQ=255)
                                                                                                                  caCGTCGGTAGACAGCGTACTGTACCAGCTGGGCGCACCGTCTCGTATGAACAGCGGTCAGACCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                          <  2:680949/142‑1 (MQ=255)
                                                                                                                      ccGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACATGGGTATGGCTGCGAAAGGTATCGGCGACAAGAGCAACGCCATGCTGAAACAGCAGCAAGAAGACGCGaa                      >  2:455521/1‑143 (MQ=255)
                                                                                                                         gTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCGTGCAGAAACAGCAGCACGGACACGCGAAACt                   >  1:877330/1‑143 (MQ=255)
                                                                                                                            gACCTCGTACTGAACCAGCTTGGCGTACCGCCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTgcg                <  1:350641/143‑1 (MQ=255)
                                                                                                                                 tCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAGGACGCGAGACGGCGCGaa            >  1:642543/1‑143 (MQ=255)
                                                                                                                                 tCGTACTCAACTGGCTGGGCCTACCGTCTCTTCTGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa            <  1:1117010/143‑1 (MQ=255)
                                                                                                                                           cccGCCGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAgc  <  2:297585/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                            
TCTGGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTCATCCAGC  >  NC_000913/4184402‑4184682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: