Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,890,491	A→C	19.4%	G14G (GGT→GGG)	yeaY ←	Slp family lipoprotein, RpoE‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,890,491	0	A	C	19.4%	116.6 / 3.6	61	G14G (GGT→GGG)	yeaY	Slp family lipoprotein, RpoE‑regulated
Reads supporting (aligned to +/- strand):  ref base A (32/15);  new base C (2/12);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 5.26e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGGCAGTAACACTTCGCCATCGCGC  >  NC_000913/1890254‑1890725                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           agaAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACCCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTa                                                                                                                                                                                                                                            <  1:284693/238‑1 (MQ=255)         ctcCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGt                                                                                                                                                                                                                                   <  1:710751/239‑1 (MQ=255)         ctcCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGt                                                                                                                                                                                                                                   <  1:413443/239‑1 (MQ=255)                cGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACCCAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCtt                                                                                                                                                                                                                            >  1:510228/1‑239 (MQ=255)                cGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGAGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCAGGCAC‑AGGGACACAACCGCTCAGACTTACCGCAAACGTACCTGCCAGTCTGCCtt                                                                                                                                                                                                                            >  2:718547/1‑239 (MQ=255)                 gTCGGTCTGGCTCCGCGGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACACCCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGcc                                                                                                                                                                                                                              <  1:991280/236‑1 (MQ=255)                  tCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTCCCTGCCAGTATGCCTTTg                                                                                                                                                                                                                          >  1:1069218/1‑239 (MQ=255)                        tGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGTACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACa                                                                                                                                                                                                                    <  1:14219/239‑1 (MQ=255)                        tGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACa                                                                                                                                                                                                                    <  1:181292/239‑1 (MQ=255)                                cTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGGCTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTg                                                                                                                                                                                                            >  1:373210/1‑239 (MQ=255)                                  gccaaGCGGTACGTGACAAATTTCAGGCCGTTTTTTCCTTTGTTGTTTTTCACCCGCAACCACTTTCCCCCCAAAGCGTGCCT‑CCTGAC‑CAGCGTACGGCTGCGCCCAACTCAGCCCCCGACCAAAATCTGGTGGGGGCGGGGACCGGTTGCTTTAAAGGGCTTCCGGCAA‑AGTGACACAACCGCTCAGAATTAGCGAAACCGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGaa                                                                                                                                                                                                          <  2:658731/235‑1 (MQ=255)                                                aactattctcaggccGAGTTTTCCTTGGCTGTTTTTGT‑CCCCAACCCCTGTCACCCGCAACCCTGCCC‑TCTGCCACACCGGACCGCTGCCGCCCACTACCCCCCCAAATAAAATTTTGTTGCGGCGTGGGACTGCGGCCTTAAATGGGGTCC‑GCACAAGTGACAAACCCCCTCAGCATTGGCGCAAACGTCCCTCCCAGTATGCCTTGGATAACATTCTTTTGAACCGCCATGGTGCGa                                                                                                                                                                                            <  2:761263/225‑1 (MQ=255)                                                     aTTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGCACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGAACAAACCGCCCACCATTAGAGCAAACGCACCTGCCAGTATGCCCTTGATAACATTCTTTTGCACCGCCATGGAGCGACTCCt                                                                                                                                                                                       >  2:947053/1‑239 (MQ=255)                                                               gtgTTTCCCCTTGTGGGTTTTATCCCGCAACCTCTTTGCCCCCAACGCG‑GCCTCCCGGCC‑CAACGTACCGCTGCGGCCCACTCACCCCCCGAACTAAATCTTTTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTtctc                                                                                                                                                                              <  2:308256/236‑1 (MQ=255)                                                               gcgTTTTCCCTTGCTGTTTTTGTACCGCACCCACTTCGCCCCCAAAGTGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCCCTCACCCCCCGAGCTAATTTTTGTTGCGGCGTGGGACTGCGGCTTTTAATGGCGCCCGCCAC‑AGTGACCCAACCGCTCAGCATTAGCGAAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTtctc                                                                                                                                                                              <  2:948585/236‑1 (MQ=255)                                                                    ttCCCTTGCTGTTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCCCCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCa                                                                                                                                                                        <  1:836769/239‑1 (MQ=255)                                                                    ttCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCCCTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGAAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCa                                                                                                                                                                        <  1:513404/239‑1 (MQ=255)                                                                           gCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGAAACAACCGCTCAGCATTAGCGCAACCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGTCATACTCtt                                                                                                                                                                 >  1:199519/1‑239 (MQ=255)                                                                                         gCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCa                                                                                                                                                                        <  1:982765/218‑1 (MQ=255)                                                                                         gCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCa                                                                                                                                                                        >  2:982765/1‑218 (MQ=255)                                                                                          cAACCACCTTGCCACCAAAGCGTGCCT‑TCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGAATGCTGCCTTTAACGGCGTGCGGCAC‑AGTGAGACAACTGCCAAGCTTTAGTGCAACCGTCCCTGCTAGTATGCCTTTGATAACATACTTTTGAACAAACATGGTGCGACTCCCTTTTTATTCAGCGCATACCATTAACAATCAATCttt                                                                                                                                                   >  2:591655/1‑238 (MQ=255)                                                                                              cACTTGCCCCCCAAAGCGTGCCC‑CCTGCC‑CAGCGTGCGGCTCCGGCGCCCTCACCACCCGAACTAAATCTTTTTCGGGCTTGGGACGGCTGCCTTTAATGGGGTCCGGCAC‑AGGGACACAACCGCTCAGCATTAGCGCAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCg                                                                                                                                              <  2:593984/239‑1 (MQ=255)                                                                                                     ccaccaAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGCGCATACTCTTAAGATTCATTCTTTGCCCGGAAGttt                                                                                                                                       >  1:793618/1‑239 (MQ=255)                                                                                                       accaAAGCGTGCCT‑CCGGAC‑CACCGTCCAGCTGCGGCGCCCTCATCACCCGAACTAAATCTTGTTGCGGCGGGGGACTGCTGCCTTTAAGGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGAAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTttct                                                                                                                                     <  1:682334/239‑1 (MQ=255)                                                                                                                                cGTACAGCTGCGGCGCCCTCTCCCCCCGACCAAAATCTTGTGGCGGCGGGGGCTTGCTCCCTTTAATGGCGTCCGGCAC‑AGTGACACACCCGCTCAGCATTAGCGAAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATg                                                                                                                               <  2:584837/222‑1 (MQ=255)                                                                                                                                cGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGtaaa                                                                                                              >  1:906107/1‑239 (MQ=255)                                                                                                                                     cgCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACACCCGCTAAGCATTAGCGCACACCTACCTGCCAGTATGCCTTTGATCAAATTCTTTTGAACCGCCATGGGGAGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCCTTGCCCGGCAGTGTCTTCCATCCGAAGTTGTTACGcaattaaa                                                                                                          >  2:236519/2‑238 (MQ=255)                                                                                                                                     atttGCGCCCCACGCATCACCCAAACAAAAGCTGGTTGCGGATTGGTACTGCTTCCTTTATTGGCTGCCGGCAC‑AGTGACCAAACCGCTCGGCATTAGCCCAAACCTACCGGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGtaaataaa                                                                                                          <  2:991696/235‑1 (MQ=255)                                                                                                                                      gcgtatgtgtaaaagagacgggAACTAAATCGTGTTGCGGCGTGGGACTGTGGCCTTTAGTGCCGCCCGGCAC‑ATTGCCACAACCGCTCAGAATTAGCGCAAACTTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAAc                                                                                                         <  2:702722/217‑1 (MQ=255)                                                                                                                                         gcggcgCACTCATCCCCCAACCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGCCACAACCGCTCAGCATTAGCGCAAACTTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGt                                                                                                     <  1:427915/239‑1 (MQ=255)                                                                                                                                          cggcgCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACgttgtt                                                                                                                     >  1:584837/1‑222 (MQ=255)                                                                                                                                          cggcgCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGt                                                                                                     >  1:282600/1‑238 (MQ=255)                                                                                                                                              gcACTCATCACCCAAACAAAATCTGTTTGCGGCGTGGGACTGCTGCCTGTAATGGCTTCCGGCAA‑GGGAACCCAACCGCTAAGCATTAGCGCAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGc                                                                                                <  2:282600/239‑1 (MQ=255)                                                                                                                                                            aaCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCCTGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGTCGTTGTTACGTAAATAAACAGGCTCGGCATGTTacaccgcc                                                                                   >  2:533825/1‑238 (MQ=255)                                                                                                                                                            aaCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTccaccgcc                                                                                   >  1:539411/1‑238 (MQ=255)                                                                                                                                                            aaCTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGGTCGGCATGTTccaccgcc                                                                                   >  1:659754/1‑238 (MQ=255)                                                                                                                                                            aaCAAAATCTTGTTGCGGCGTGGGACTGCTGCTTTTAATGGCGTCCGGCCC‑GGTGCCACAACCTCTCAGCATTAGCGCAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTccaccgcca                                                                                  <  1:588021/239‑1 (MQ=255)                                                                                                                                                             acaaaaTCTTGTGGCGGCGTGGGATTGCTGCCTTTAATGGCGTCCGCCAC‑AGGGACACAACCGTTCAGCATTACCCCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTccaccgcca                                                                                  <  1:947053/235‑1 (MQ=255)                                                                                                                                                                      tgttgCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCa                                                                                               >  1:702722/1‑216 (MQ=255)                                                                                                                                                                             gcgTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTttctt                                                                                                                                    >  1:28388/1‑172 (MQ=255)                                                                                                                                                                                       gctgcCTTTAAGGGCTTCCGGCAC‑ATTGCCACAACCGCTCAGCATAAGCGCAAACGTCCCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGac                                                       <  1:796282/239‑1 (MQ=255)                                                                                                                                                                                        ctgcCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGCGCGACACCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTGTCTTCCATGCGTCGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTATACTCAGCAAACATCTgcc                                                       >  2:1098817/1‑236 (MQ=255)                                                                                                                                                                                             tttAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCaa                                                 >  1:55624/1‑239 (MQ=255)                                                                                                                                                                                                       tCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCat                                       >  1:725477/1‑239 (MQ=255)                                                                                                                                                                                                       tCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGAGCGACTCCTTTTTTTCTCAGGACATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGGTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCat                                       >  2:1096870/1‑239 (MQ=255)                                                                                                                                                                                                       tCCAGCAC‑AGTGACACAACCGCTCAGCATTACCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTCCCACTCCTTTTTTTCTCAGACCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCCTGCGCCGTTGTTACGTAAATAAACCGGCTCGGCATGTTCCACCGCCACCGCTTTACCATCAGCAAACATCTGACAAGCAATCGGCACCat                                       >  2:100743/1‑239 (MQ=255)                                                                                                                                                                                                                    acacAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTcagcagc                           >  2:256986/1‑239 (MQ=255)                                                                                                                                                                                                                     caataCCGCTCTTTATTAGCTCACACGTCCCTTCCAGTATGCCTTTGACAACTTTTTTTTGAACCGCCATGGTGCGCCTCCTTGTTTTCTCATGGCATACTCTTAAGATTCATTCTTTGCTCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTcagcagca                          <  2:659754/235‑1 (MQ=255)                                                                                                                                                                                                                       aatccgccctgcttaaccacAAACTTCCCTGCCAGTATGCTTTTGATAACATTCTTTTGAACCGCCATGATGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAgcagg                        <  2:640032/220‑1 (MQ=255)                                                                                                                                                                                                                          ccGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAgcaggcag                     >  1:1079572/1‑239 (MQ=255)                                                                                                                                                                                                                          ccGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATAATCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCCCCGCCACCGTTTTACCCTCCGAAAACATCTGACACGCAATCGGCAGCCTATCTTAAGCCgcaggcag                     >  2:237725/1‑239 (MQ=255)                                                                                                                                                                                                                            gCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAAGCGCCATGGTGCGACTCCTTTTTTTCTCAGGGGATACTCTTTAGCTTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGTCATGTTCCCCCGCCACCGTTTTACCCTCAGCAAACATCTACCACGCAATCCGCAGCATTCCTTAACCATCAGgtagta                   >  1:961013/1‑239 (MQ=255)                                                                                                                                                                                                                             cTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACGGCCATGGTGCGACTCCTTTTTTTCTCACGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAACAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGCCAGTa                   >  2:663522/1‑238 (MQ=255)                                                                                                                                                                                                                             cTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAAACGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTTTTCCATGCGACGCTGTTACGTAAATAACCCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTAAGCAAACATTTGACACCAAATCGGCAGCATATCTTCAACAgcagccagca                   >  2:455240/1‑236 (MQ=255)                                                                                                                                                                                                                                aGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGGCAGTAac                 >  1:574325/1‑237 (MQ=255)                                                                                                                                                                                                                                        ctcAAACGTACCTTCAAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTATGTAATTAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCACACATCTGACACGCAATCGGCAGCATATCTTTCTCAGCAGGCCGCAACACCTCCCCa        >  2:290786/3‑238 (MQ=255)                                                                                                                                                                                                                                            aaCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTcc                                                                                                                                                                                        <  2:447323/58‑1 (MQ=255)                                                                                                                                                                                                                                            aaCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTcc                                                                                                                                                                                        >  1:447323/1‑58 (MQ=255)                                                                                                                                                                                                                                            aaCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGGCAGTAaca                >  1:739952/1‑226 (MQ=255)                                                                                                                                                                                                                                            aaCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGGCAGTAACACTTCGCCATcgcg   <  1:521086/239‑1 (MQ=255)                                                                                                                                                                                                                                            aaCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGGCAGTAACACTTCGCCATcgc    >  2:521086/1‑238 (MQ=255)                                                                                                                                                                                                                                             aCGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGGCAGTAACACTTCGCCATCgcgc  <  1:520515/239‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                           AGAAGGTTCTCCCAGCGTCGGTCTGGCTCCGCTGTCCAGCGGTACGGTAGCAATTTCCAGGCGAGTTTTCCCTTGCTGGTTTTGTACCGCAACCACTTTGCCACCAAAGCGTGCCT‑CCTGAC‑CAACGTACAGCTGCGGCGCACTCATCACCCGAACTAAATCTTGTTGCGGCGTGGGACTGCTGCCTTTAATGGCGTCCGGCAC‑AGTGACACAACCGCTCAGCATTAGCGCAAACGTACCTGCCAGTATGCCTTTGATAACATTCTTTTGAACCGCCATGGTGCGACTCCTTTTTTTCTCAGGGCATACTCTTAAGATTCATTCTTTGCCCGGAAGTTTCTTCCATGCGACGTTGTTACGTAAATAAACCGGTTCGGCATGTTCCACCGCCACCGTTTTACCCTCAGCAAACATCTGACACGCAATCGGCAGCATATCTTCAGCAGCAGGCAGTAACACTTCGCCATCGCGC  >  NC_000913/1890254‑1890725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑