Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,467 A→G 16.5% S3G (AGC→GGC)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4670AG16.5% 110.8 / 4.3 63S3G (AGC→GGC) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (12/38);  new base G (6/4);  total (19/44)
Fisher's exact test for biased strand distribution p-value = 5.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAA  >  NC_000913/3206233‑3206705
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
cTAAAGGCCAGCTATTCAACATCGTTAATTATCCGCAGTTGTGATAAGCGCTGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                                                                                                                                                              <  2:863546/238‑1 (MQ=255)
 tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                             <  1:1050312/238‑1 (MQ=255)
 tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                             <  2:129307/238‑1 (MQ=255)
 tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                             <  2:312911/238‑1 (MQ=255)
 tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                             <  1:457267/238‑1 (MQ=255)
 tAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAGTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                                                                                                                                             <  1:256212/238‑1 (MQ=255)
        cAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                                                                                                                                                      <  1:468824/238‑1 (MQ=255)
                    aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                            <  2:1116517/236‑1 (MQ=255)
                    aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                            <  2:24839/236‑1 (MQ=255)
                    aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAATTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                            <  1:9159/236‑1 (MQ=255)
                     tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATACAAAAAATGGGGTTGCCATGATTAAGGGAAGGAATAAACAAAAGGCAGt                                                                                                                                                                                                                        >  1:184062/1‑239 (MQ=255)
                     tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTAAGTTTTCTCGTCAAAAAAAAAAAATGGAGGTGGCATGAGAGGCGGAAATAATAAACaaaacagaag                                                                                                                                                                                                                        >  2:943734/1‑231 (MQ=255)
                        ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCCGACAAAAACAAAAAATAGAGGTGCCATTGTTGGCGGAAATAAAAAACAACAAGCacggga                                                                                                                                                                                                                     >  1:1095243/1‑234 (MQ=255)
                                              aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGTGGGGAAAGAAAAAACAACAAGCAGGGAATAAGATGaaaagaaatttcgcc                                                                                                                                                                                              >  1:363770/1‑225 (MQ=255)
                                              aGCGCAGTGTATTACGCAAAACATTGCCATTCCCTGCTCTGCCGCATTATTCATTCTTATTTTCGTTTTGATTCACGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGGGAATTTTTCCAGACAAAGACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAGTGTCGCt                                                                                                                                                                                              <  2:302195/239‑1 (MQ=255)
                                                       tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                    <  1:331930/208‑1 (MQ=255)
                                                          ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATACAAAAAATGGAGTTGTCCTGATGAGAGAAAGGAAAAAACAACAAGCAGGGAATAAGTTTACAGAGATTGTTGCTAACTTTAACGc                                                                                                                                                                                   >  1:906499/1‑238 (MQ=255)
                                                           gccaaaaaatttGCCATTCCATTCTGTGGCGAAAAATTCATTTTTAGTCTGGTGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCCCTATTTTAGTGAAAGTTAGTGGGGGGAAATTTTCCAATACAAATACAAAATGTGTAGTTGCGTAAATGGGCAAAAGTAATAAGAAACAGGAGGATTCTAAGTTGTGAGGATTTTTCGTTAACTTTACCGCCa                                                                                                                                                                                 <  2:586948/229‑1 (MQ=255)
                                                                      tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGAAAAAAAAAAAAAAATGAGGTTGCATGATTAGGGAAAGTAATAAACAACACACAGAGAAAAAAGTTACAGAGATTTTTTCTTACCTTTCCGCCATGAttttcta                                                                                                                                                                       >  2:60242/1‑234 (MQ=255)
                                                                       gCCATCCCCTGCCCTGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGTTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACGGGAGTTGCAATGATGAGAGAAAGTAATAAGAAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTAccc                                                                                                                                                                     <  1:1142186/239‑1 (MQ=255)
                                                                           tcccttGCTCTGGCGCATTATTCAGTCTTATTTTCGCGTTGATTCACGACCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCATATTTTTAGTGGTTTGTTTTGGCGGTGATTTTTTCAAGAAAAATACAAAACTGGGAGTTGCCATGAGGTGCAAAATTAATAAGCAACAGGAAGTGAATAAGTGGACAGAGATTTCCGATAACTTTACCGCCATGATTTCTACCCGAAt                                                                                                                                                                 <  2:1106716/234‑1 (MQ=255)
                                                                             cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGACATTATTGGCGGAAGGAATAAGGAAACAGCAGGGAATAAGTTGAGAAAGGTTGTTGCCTACTTTATCGCCCCGAttttttacccaatg                                                                                                                                                                >  1:1109367/1‑227 (MQ=255)
                                                                              ccggCTCTGGCTAAAATTTCATTCTTATTTCCGATTTAATTAACGAAGTTTTAATTAAATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTATTTTGTGGTGTTAAATTTTTCAAGACAAATAAAAAAACGGGAGTTGCCATGATGGGCTAAAGTAATAAGCAACAGCCATTGAATAAGTTAACAGGGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGcc                                                                                                                                                              <  2:497370/236‑1 (MQ=255)
                                                                                             tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                               <  1:943734/239‑1 (MQ=255)
                                                                                                 cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATACAAAAAAAAGAGGTGCCATGATGAGCGAAAGGAATAAAAAACAGGCAGAGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCAATATTTCTAACCCAATGCCTGATGACGCGGGGGGTaaa                                                                                                                                           >  1:719784/1‑239 (MQ=255)
                                                                                                      ttATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                      <  1:60242/239‑1 (MQ=255)
                                                                                                      ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                      <  1:353221/239‑1 (MQ=255)
                                                                                                       tATTTTCGCGTTGATTCACGAATTTTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                     <  1:87828/239‑1 (MQ=255)
                                                                                                       tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGGTGGCATGATGAGCGGAAGGAATAAGCAAAAAGGAGAGAATAAGATGAAAGAGATTTTGGCTAAATTTTACGCCCTGATTTCTTACCCAAAGCCTGAGTAAGGGGGGGGAAAACTaaaa                                                                                                                                     >  1:964194/1‑239 (MQ=255)
                                                                                                       tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAAGCAAAAATAAAAACTGGAGTTGCCGTGATGGGGGAAAGGAATAAACAAAAAGAAGGGAAATAAATGGAAGAAATTTTCGCTTAATTTACCCCCCTGATTTTTTCCCGGAAGACTGATTACGTTGGGGGTAAACAaaaa                                                                                                                                     >  1:142788/1‑239 (MQ=255)
                                                                                                       aatttcggtcttGCTTCCCAAATTGTAAATTACATTTTCCGGGTGGAAAAAATAAAAAAACCACTTTTTTAGGGGTAGTTTGGGGCGGTGAATTTTTCCAGACAAATAAAAAACCGGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                     <  2:780063/229‑1 (MQ=255)
                                                                                                                  tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                               <  1:57911/218‑1 (MQ=255)
                                                                                                                  tGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGTTATTTTGTGGCGGTAATTTTTTCCAAACAAATACAAAAACTGGAGTTGCCATAATGAGCAAAAGTAATAAGCAACGGGGAGTAAATAAGTTAACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                                                          <  1:193005/239‑1 (MQ=255)
                                                                                                                         caaaCTGTGAATTACATATTCACGGCGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTGGTGGCGGGGAATTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAACAACAGGCAGTAAATAGGTCGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                   <  1:859525/237‑1 (MQ=255)
                                                                                                                            aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAATGGAGTTTCCCTTGTGGGCGGAAGAAAAAAAAAAAAAGAAAAGAATAAAATGACAGAGATTGTCGCTAACTTTTCACCCCAGATTTCTTACCGAATACCCGATGACGTGGTGGGTTAACAAAAAcctgtctcttttacaaatctg                                                                                                                >  2:57911/1‑219 (MQ=255)
                                                                                                                             cttttAATTGCATTTTCCGGGTGGAAAAAAAAAAAAACCCACATTTTGTGGGGTAGTGTGGGGGGGTGATTTTTTCCAGAAAAAAAAAAAAACGTGAGTGGCCATGAGGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgt                                                                                                               <  2:906499/236‑1 (MQ=255)
                                                                                                                               gTTAATTACATATTCACGGGAAAAAAAAATATAAAACCACATTTTTGTGGGTAGTTGTTGGCGGTTAATTTTTCAAAACAAATACAAAAACTGGAGTTGCCAGGTGGAGCAAAAATAAAAAGCACTAGGCGTTAAATAAGTGGAAAGAGATTGTCGTTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg                                                                                                             <  2:964194/239‑1 (MQ=255)
                                                                                                                                ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAATAAAGTTACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTTACCGAATGCATGTTGACGTGGTGGGTAAAATAAAAAAACTAAAAGATGGCGCAAcgtcg                                                                                                             >  1:925507/1‑238 (MQ=255)
                                                                                                                                  aaatatcatttcgaggtgaaaaaaaaTATAAAACCCACTTTTAAGTGGTAGTTTGTGGTTGTGAATTTTACAAGACAAAAACAAAAACGGGTGTTGCCATGATTAGCGAAAGTAATAAGCAACAGGTAGTGAATAATTAGACAGAGTTTGTCGAAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtct                                                                                                          <  2:997264/219‑2 (MQ=255)
                                                                                                                                  aaTGACATGTTCCCGGTGGAAAAAAATAAAAAACCACATTTTAGGGTGTAGTTTGGGACGGTGAATTTTCCCAAAAAAATAAAAAAACTGGAGTTCCAGTAATGCGCAAAAGTAAAAACAAACGGGCAGTAAATAATTGGACAGAGTTTGCCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                          <  2:142788/239‑1 (MQ=255)
                                                                                                                                    ttACATATTCGCGGGGGAAAAAAATAAAAAACCACATTTGTATTGGTGTTTTGTGGCGGTGAATTTTTCCAAAAAAAAAAAAAACCGGGAGTTTCAATGAAGAGAAAAAGTAAAAACAAAGGGGCAGTAAATAATGGGAAAGAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                          <  2:872854/237‑1 (MQ=255)
                                                                                                                                        atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAATAAAAAAATGAGTTGTCATTATGGGAGAAAATATAAAAAAACAGGAAGTGAAAAAAGTGACAGAGATTGTCGCTAACTTTACACCCCAGATTTTTACCCACACACCTGTTGACATGGGGGGTGAAATCAAAAAGCTAAAAGATGCGGGAAACTCCTAGATgggg                                                                                                    >  2:392203/1‑239 (MQ=255)
                                                                                                                                         tatTCAGGGTGGAAAAAAAAATAAACCCATTTTTTGAGTGGTGGTTTGGGGCGGTCAATTTTGCCAAAAAAAAAAAAAAACGGGAGTTACAATGATGGGAGAAAGTAATAAGCAAGAGGCACTGAAAGCGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                   <  1:827442/239‑1 (MQ=255)
                                                                                                                                         tatTCACGGTGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGATTTTTACCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                   <  1:662820/239‑1 (MQ=255)
                                                                                                                                         tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                   <  1:903447/239‑1 (MQ=255)
                                                                                                                                          atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTCCACAACAAAAAAAAAAAATGGGTTTGCATGATGAGGAGAAAAAATAAAAAACAGGCAGTGAATAAGATGATAGAGAATGTCGCTAACTTTAACGCCATGATTTCTACCCCAATGGCTGGTGACGGGGGGGGTAAACTCAAAAAAATAAAGGGAGCCGAAAAGGAGTCGGTgggggg                                                                                                  >  2:183559/1‑237 (MQ=255)
                                                                                                                                           gacACGGTGGCAAAAAAAAAAAAACAAAATTTTTATGGGTAGTGTGTGGGGGTGAATTTTTCAAGCAAAATAAAAAATCTGGAGTTGCAATGAGGAGAGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGTTAATTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                   <  2:719784/235‑1 (MQ=255)
                                                                                                                                             cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAAGGAATAAGATTACAGAGAATTTCGCTTACTTTACCGCCATGATTTTTACCCGAAAGCCCGATGAGGTGGGGGATAAACTAAAAAAACTAAAAGAAGCCCGAAAGTCGTCGATGGGGGAAAt                                                                                               >  1:871285/1‑239 (MQ=255)
                                                                                                                                                 tggaaaaaaaaTAAAAAAACCATTTGTTGTTGGTGGTGTGTGGGGGTAAATTTTTCGAAACAAAAACAAAAACTGGAGTTACTATGATGGGCGAAATAAATAAGCAACAGGAAGTTAATAAGTTCATACAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACTTCGTCGATGGGGAAAATTAt                                                                                            <  2:174226/233‑1 (MQ=255)
                                                                                                                                                  tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAATTGAGTTGCCCTGATGAGCGGAAGGAATAAACAAAAGGCCGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTTTTCCCGCAAGCCTGATGAAGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGGAAAGTCGTCGCTGGGGGAAATTATCt                                                                                          >  1:832264/1‑239 (MQ=255)
                                                                                                                                                    gaaaaaaaaaaaaaCCCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                         <  1:1111938/236‑1 (MQ=255)
                                                                                                                                                    gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAACAGGCAGTGAATAAAATTACAGAGATTGTAGCTAACTTTAACGCCATGATTTCTTCCCGCATGCCTGATGACGGGGTGGGTAAAATAAAAAAGCTAAAAGAAGGCGAAACGTCGTCGGTGGGGGAAATtatata                                                                                         >  1:1148268/1‑238 (MQ=255)
                                                                                                                                                            tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                                                                     <  1:691234/218‑1 (MQ=255)
                                                                                                                                                              taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCCAAAGCCTGATGACGGGGGGGGTAAAATAAAAAAGCTAAAGGATGCCGAAACGTCGTCGATGGGGGAAAATATTTACCATACCATGt                                                                              >  1:258223/1‑239 (MQ=255)
                                                                                                                                                                      caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGAAAAAAAAAAAAAATGGAGGTGGCATGTTGAGGGGAAGTAATAAGAAAAAAGAAGTGAAAAAAGTGAAAGAGATTGTGTGTAAATTTTACGCCATGAATTCTACCCGAATACCTTATGAAGTGGTGGGTAAAATAAAAAAGCTAAAGAAAGACGGAACGTCGTCGATGGGGGAAATTAtttttgtactttatacacatctg                                                                      >  2:691234/1‑217 (MQ=255)
                                                                                                                                                                                    aGTTTGTGGGGGAGAATTTTTCCAAAAAAATAAAAAAACTGGAGTTGCAATGAGGAACGAAAGAAATAAGCAACAGGCGGTAAAGAAGTGGACAGATATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                         <  2:493407/239‑1 (MQ=255)
                                                                                                                                                                                    aGTTGGTGGAGGTAAATTTGACAACAAAAATACAAAAACTGAAGTTGCCATGATGAGCGAAAGGAATAACAAACAGCCAAGAAATAAGTTGACTGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                         <  2:533339/239‑1 (MQ=255)
                                                                                                                                                                                      tttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                    <  1:113038/210‑1 (MQ=255)
                                                                                                                                                                                                      tttCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                       <  1:796851/239‑1 (MQ=255)
                                                                                                                                                                                                      gtgccAAACAAAGAAAAAAACTGGAGTTGCCATAATGAGCGAAAGAAAAAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                       <  2:363770/236‑1 (MQ=255)
                                                                                                                                                                                                                         actgggAGTAGCAATTAGGAGCGAAAGAAATAGGCAACAGGAAGGAAATAAGTTGACAGAGATTTCCGCTAATTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                     <  2:756761/235‑1 (MQ=255)
                                                                                                                                                                                                                         aaCGGGAGTTGCCAAGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                     <  1:733907/239‑1 (MQ=255)
                                                                                                                                                                                                                         aaCGGGAGGTGCCATGATGAGCGAAAGTAATAATCAACGGGCAGTAAATAATTTACCAGAGTTTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                     <  2:925507/239‑1 (MQ=255)
                                                                                                                                                                                                                                            aGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAACTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaa  >  1:1074648/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
CTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAA  >  NC_000913/3206233‑3206705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: