Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,481 G→A 29.6% K7K (AAG→AAA ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4810GA29.6% 68.0 / 15.2 61K7K (AAG→AAAttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (7/33);  new base A (15/2);  total (22/39)
Fisher's exact test for biased strand distribution p-value = 7.46e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCG  >  NC_000913/3206253‑3206717
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                         <  2:24839/236‑1 (MQ=255)
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                         <  2:1116517/236‑1 (MQ=255)
aTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAATTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                                                                                                                                                         <  1:9159/236‑1 (MQ=255)
 tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAATACAAAAAATGGGGTTGCCATGATTAAGGGAAGGAATAAACAAAAGGCAGt                                                                                                                                                                                                                                     >  1:184062/1‑239 (MQ=255)
 tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTAAGTTTTCTCGTCAAAAAAAAAAAATGGAGGTGGCATGAGAGGCGGAAATAATAAACaaaacagaag                                                                                                                                                                                                                                     >  2:943734/1‑231 (MQ=255)
    ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTCCGACAAAAACAAAAAATAGAGGTGCCATTGTTGGCGGAAATAAAAAACAACAAGCacggga                                                                                                                                                                                                                                  >  1:1095243/1‑234 (MQ=255)
                          aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGTGGGGAAAGAAAAAACAACAAGCAGGGAATAAGATGaaaagaaatttcgcc                                                                                                                                                                                                           >  1:363770/1‑225 (MQ=255)
                          aGCGCAGTGTATTACGCAAAACATTGCCATTCCCTGCTCTGCCGCATTATTCATTCTTATTTTCGTTTTGATTCACGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGGGAATTTTTCCAGACAAAGACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAGTGTCGCt                                                                                                                                                                                                           <  2:302195/239‑1 (MQ=255)
                                   tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                                                                                 <  1:331930/208‑1 (MQ=255)
                                      ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATACAAAAAATGGAGTTGTCCTGATGAGAGAAAGGAAAAAACAACAAGCAGGGAATAAGTTTACAGAGATTGTTGCTAACTTTAACGc                                                                                                                                                                                                >  1:906499/1‑238 (MQ=255)
                                       gccaaaaaatttGCCATTCCATTCTGTGGCGAAAAATTCATTTTTAGTCTGGTGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCCCTATTTTAGTGAAAGTTAGTGGGGGGAAATTTTCCAATACAAATACAAAATGTGTAGTTGCGTAAATGGGCAAAAGTAATAAGAAACAGGAGGATTCTAAGTTGTGAGGATTTTTCGTTAACTTTACCGCCa                                                                                                                                                                                              <  2:586948/229‑1 (MQ=255)
                                                  tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGAAAAAAAAAAAAAAATGAGGTTGCATGATTAGGGAAAGTAATAAACAACACACAGAGAAAAAAGTTACAGAGATTTTTTCTTACCTTTCCGCCATGAttttcta                                                                                                                                                                                    >  2:60242/1‑234 (MQ=255)
                                                   gCCATCCCCTGCCCTGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGTTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACGGGAGTTGCAATGATGAGAGAAAGTAATAAGAAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTAccc                                                                                                                                                                                  <  1:1142186/239‑1 (MQ=255)
                                                       tcccttGCTCTGGCGCATTATTCAGTCTTATTTTCGCGTTGATTCACGACCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCATATTTTTAGTGGTTTGTTTTGGCGGTGATTTTTTCAAGAAAAATACAAAACTGGGAGTTGCCATGAGGTGCAAAATTAATAAGCAACAGGAAGTGAATAAGTGGACAGAGATTTCCGATAACTTTACCGCCATGATTTCTACCCGAAt                                                                                                                                                                              <  2:1106716/234‑1 (MQ=255)
                                                         cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGACATTATTGGCGGAAGGAATAAGGAAACAGCAGGGAATAAGTTGAGAAAGGTTGTTGCCTACTTTATCGCCCCGAttttttacccaatg                                                                                                                                                                             >  1:1109367/1‑227 (MQ=255)
                                                          ccggCTCTGGCTAAAATTTCATTCTTATTTCCGATTTAATTAACGAAGTTTTAATTAAATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTATTTTGTGGTGTTAAATTTTTCAAGACAAATAAAAAAACGGGAGTTGCCATGATGGGCTAAAGTAATAAGCAACAGCCATTGAATAAGTTAACAGGGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGcc                                                                                                                                                                           <  2:497370/236‑1 (MQ=255)
                                                                         tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  1:943734/239‑1 (MQ=255)
                                                                             cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATACAAAAAAAAGAGGTGCCATGATGAGCGAAAGGAATAAAAAACAGGCAGAGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCAATATTTCTAACCCAATGCCTGATGACGCGGGGGGTaaa                                                                                                                                                        >  1:719784/1‑239 (MQ=255)
                                                                                  ttATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                   <  1:60242/239‑1 (MQ=255)
                                                                                  ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                   <  1:353221/239‑1 (MQ=255)
                                                                                   tATTTTCGCGTTGATTCACGAATTTTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                  <  1:87828/239‑1 (MQ=255)
                                                                                   tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGGTGGCATGATGAGCGGAAGGAATAAGCAAAAAGGAGAGAATAAGATGAAAGAGATTTTGGCTAAATTTTACGCCCTGATTTCTTACCCAAAGCCTGAGTAAGGGGGGGGAAAACTaaaa                                                                                                                                                  >  1:964194/1‑239 (MQ=255)
                                                                                   tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAAGCAAAAATAAAAACTGGAGTTGCCGTGATGGGGGAAAGGAATAAACAAAAAGAAGGGAAATAAATGGAAGAAATTTTCGCTTAATTTACCCCCCTGATTTTTTCCCGGAAGACTGATTACGTTGGGGGTAAACAaaaa                                                                                                                                                  >  1:142788/1‑239 (MQ=255)
                                                                                   aatttcggtcttGCTTCCCAAATTGTAAATTACATTTTCCGGGTGGAAAAAATAAAAAAACCACTTTTTTAGGGGTAGTTTGGGGCGGTGAATTTTTCCAGACAAATAAAAAACCGGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                  <  2:780063/229‑1 (MQ=255)
                                                                                              tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                            <  1:57911/218‑1 (MQ=255)
                                                                                              tGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGTTATTTTGTGGCGGTAATTTTTTCCAAACAAATACAAAAACTGGAGTTGCCATAATGAGCAAAAGTAATAAGCAACGGGGAGTAAATAAGTTAACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                                                                       <  1:193005/239‑1 (MQ=255)
                                                                                                     caaaCTGTGAATTACATATTCACGGCGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTGGTGGCGGGGAATTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAACAACAGGCAGTAAATAGGTCGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                <  1:859525/237‑1 (MQ=255)
                                                                                                        aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAATGGAGTTTCCCTTGTGGGCGGAAGAAAAAAAAAAAAAGAAAAGAATAAAATGACAGAGATTGTCGCTAACTTTTCACCCCAGATTTCTTACCGAATACCCGATGACGTGGTGGGTTAACAAAAAcctgtctcttttacaaatctg                                                                                                                             >  2:57911/1‑219 (MQ=255)
                                                                                                         cttttAATTGCATTTTCCGGGTGGAAAAAAAAAAAAACCCACATTTTGTGGGGTAGTGTGGGGGGGTGATTTTTTCCAGAAAAAAAAAAAAACGTGAGTGGCCATGAGGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgt                                                                                                                            <  2:906499/236‑1 (MQ=255)
                                                                                                           gTTAATTACATATTCACGGGAAAAAAAAATATAAAACCACATTTTTGTGGGTAGTTGTTGGCGGTTAATTTTTCAAAACAAATACAAAAACTGGAGTTGCCAGGTGGAGCAAAAATAAAAAGCACTAGGCGTTAAATAAGTGGAAAGAGATTGTCGTTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg                                                                                                                          <  2:964194/239‑1 (MQ=255)
                                                                                                            ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAATAAAGTTACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTTACCGAATGCATGTTGACGTGGTGGGTAAAATAAAAAAACTAAAAGATGGCGCAAcgtcg                                                                                                                          >  1:925507/1‑238 (MQ=255)
                                                                                                              aaatatcatttcgaggtgaaaaaaaaTATAAAACCCACTTTTAAGTGGTAGTTTGTGGTTGTGAATTTTACAAGACAAAAACAAAAACGGGTGTTGCCATGATTAGCGAAAGTAATAAGCAACAGGTAGTGAATAATTAGACAGAGTTTGTCGAAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtct                                                                                                                       <  2:997264/219‑2 (MQ=255)
                                                                                                              aaTGACATGTTCCCGGTGGAAAAAAATAAAAAACCACATTTTAGGGTGTAGTTTGGGACGGTGAATTTTCCCAAAAAAATAAAAAAACTGGAGTTCCAGTAATGCGCAAAAGTAAAAACAAACGGGCAGTAAATAATTGGACAGAGTTTGCCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                       <  2:142788/239‑1 (MQ=255)
                                                                                                                ttACATATTCGCGGGGGAAAAAAATAAAAAACCACATTTGTATTGGTGTTTTGTGGCGGTGAATTTTTCCAAAAAAAAAAAAAACCGGGAGTTTCAATGAAGAGAAAAAGTAAAAACAAAGGGGCAGTAAATAATGGGAAAGAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                       <  2:872854/237‑1 (MQ=255)
                                                                                                                    atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAATAAAAAAATGAGTTGTCATTATGGGAGAAAATATAAAAAAACAGGAAGTGAAAAAAGTGACAGAGATTGTCGCTAACTTTACACCCCAGATTTTTACCCACACACCTGTTGACATGGGGGGTGAAATCAAAAAGCTAAAAGATGCGGGAAACTCCTAGATgggg                                                                                                                >  2:392203/1‑239 (MQ=255)
                                                                                                                     tatTCAGGGTGGAAAAAAAAATAAACCCATTTTTTGAGTGGTGGTTTGGGGCGGTCAATTTTGCCAAAAAAAAAAAAAAACGGGAGTTACAATGATGGGAGAAAGTAATAAGCAAGAGGCACTGAAAGCGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                               <  1:827442/239‑1 (MQ=255)
                                                                                                                     tatTCACGGTGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGATTTTTACCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                               <  1:662820/239‑1 (MQ=255)
                                                                                                                     tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                               <  1:903447/239‑1 (MQ=255)
                                                                                                                      atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTCCACAACAAAAAAAAAAAATGGGTTTGCATGATGAGGAGAAAAAATAAAAAACAGGCAGTGAATAAGATGATAGAGAATGTCGCTAACTTTAACGCCATGATTTCTACCCCAATGGCTGGTGACGGGGGGGGTAAACTCAAAAAAATAAAGGGAGCCGAAAAGGAGTCGGTgggggg                                                                                                              >  2:183559/1‑237 (MQ=255)
                                                                                                                       gacACGGTGGCAAAAAAAAAAAAACAAAATTTTTATGGGTAGTGTGTGGGGGTGAATTTTTCAAGCAAAATAAAAAATCTGGAGTTGCAATGAGGAGAGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGTTAATTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                               <  2:719784/235‑1 (MQ=255)
                                                                                                                         cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAAGGAATAAGATTACAGAGAATTTCGCTTACTTTACCGCCATGATTTTTACCCGAAAGCCCGATGAGGTGGGGGATAAACTAAAAAAACTAAAAGAAGCCCGAAAGTCGTCGATGGGGGAAAt                                                                                                           >  1:871285/1‑239 (MQ=255)
                                                                                                                             tggaaaaaaaaTAAAAAAACCATTTGTTGTTGGTGGTGTGTGGGGGTAAATTTTTCGAAACAAAAACAAAAACTGGAGTTACTATGATGGGCGAAATAAATAAGCAACAGGAAGTTAATAAGTTCATACAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACTTCGTCGATGGGGAAAATTAt                                                                                                        <  2:174226/233‑1 (MQ=255)
                                                                                                                              tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAATTGAGTTGCCCTGATGAGCGGAAGGAATAAACAAAAGGCCGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTTTTCCCGCAAGCCTGATGAAGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGGAAAGTCGTCGCTGGGGGAAATTATCt                                                                                                      >  1:832264/1‑239 (MQ=255)
                                                                                                                                gaaaaaaaaaaaaaCCCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                                     <  1:1111938/236‑1 (MQ=255)
                                                                                                                                gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAACAGGCAGTGAATAAAATTACAGAGATTGTAGCTAACTTTAACGCCATGATTTCTTCCCGCATGCCTGATGACGGGGTGGGTAAAATAAAAAAGCTAAAAGAAGGCGAAACGTCGTCGGTGGGGGAAATtatata                                                                                                     >  1:1148268/1‑238 (MQ=255)
                                                                                                                                        tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                                                                                 <  1:691234/218‑1 (MQ=255)
                                                                                                                                          taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCCAAAGCCTGATGACGGGGGGGGTAAAATAAAAAAGCTAAAGGATGCCGAAACGTCGTCGATGGGGGAAAATATTTACCATACCATGt                                                                                          >  1:258223/1‑239 (MQ=255)
                                                                                                                                                  caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGAAAAAAAAAAAAAATGGAGGTGGCATGTTGAGGGGAAGTAATAAGAAAAAAGAAGTGAAAAAAGTGAAAGAGATTGTGTGTAAATTTTACGCCATGAATTCTACCCGAATACCTTATGAAGTGGTGGGTAAAATAAAAAAGCTAAAGAAAGACGGAACGTCGTCGATGGGGGAAATTAtttttgtactttatacacatctg                                                                                  >  2:691234/1‑217 (MQ=255)
                                                                                                                                                                aGTTTGTGGGGGAGAATTTTTCCAAAAAAATAAAAAAACTGGAGTTGCAATGAGGAACGAAAGAAATAAGCAACAGGCGGTAAAGAAGTGGACAGATATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                     <  2:493407/239‑1 (MQ=255)
                                                                                                                                                                aGTTGGTGGAGGTAAATTTGACAACAAAAATACAAAAACTGAAGTTGCCATGATGAGCGAAAGGAATAACAAACAGCCAAGAAATAAGTTGACTGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                     <  2:533339/239‑1 (MQ=255)
                                                                                                                                                                  tttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                                <  1:113038/210‑1 (MQ=255)
                                                                                                                                                                                  tttCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                   <  1:796851/239‑1 (MQ=255)
                                                                                                                                                                                  gtgccAAACAAAGAAAAAAACTGGAGTTGCCATAATGAGCGAAAGAAAAAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                   <  2:363770/236‑1 (MQ=255)
                                                                                                                                                                                                     actgggAGTAGCAATTAGGAGCGAAAGAAATAGGCAACAGGAAGGAAATAAGTTGACAGAGATTTCCGCTAATTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                 <  2:756761/235‑1 (MQ=255)
                                                                                                                                                                                                     aaCGGGAGTTGCCAAGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                 <  1:733907/239‑1 (MQ=255)
                                                                                                                                                                                                     aaCGGGAGGTGCCATGATGAGCGAAAGTAATAATCAACGGGCAGTAAATAATTTACCAGAGTTTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                 <  2:925507/239‑1 (MQ=255)
                                                                                                                                                                                                                        aGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAACTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaa              >  1:1074648/1‑239 (MQ=255)
                                                                                                                                                                                                                                  ataaGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc     >  2:531185/1‑238 (MQ=255)
                                                                                                                                                                                                                                  ataaGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc     >  1:795015/1‑238 (MQ=255)
                                                                                                                                                                                                                                  ataaGCAACAGGCAGTAAATAAGTTGACAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc     <  1:53372/239‑1 (MQ=255)
                                                                                                                                                                                                                                    aaGAAACAGGCATGAAATAAGTTGACAGAGATTTTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg  <  1:733017/239‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
ATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCG  >  NC_000913/3206253‑3206717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: