Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,504 C→A 15.0% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,5040CA15.0% 137.0 / 2.7 66T15K (ACA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (21/30);  new base A (3/7);  total (27/39)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAA  >  NC_000913/3206279‑3206742
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
aGCGCAGTGTATTACGCAAAACATTGCCATTCCCTGCTCTGCCGCATTATTCATTCTTATTTTCGTTTTGATTCACGAACTGTTAATTAAATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGGGGCGGGGAATTTTTCCAGACAAAGACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAGTGTCGCt                                                                                                                                                                                                                                    <  2:302195/239‑1 (MQ=255)
            ttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAATACAAAAAATGGAGTTGTCCTGATGAGAGAAAGGAAAAAACAACAAGCAGGGAATAAGTTTACAGAGATTGTTGCTAACTTTAACGc                                                                                                                                                                                                                         >  1:906499/1‑238 (MQ=255)
             gccaaaaaatttGCCATTCCATTCTGTGGCGAAAAATTCATTTTTAGTCTGGTGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCCCTATTTTAGTGAAAGTTAGTGGGGGGAAATTTTCCAATACAAATACAAAATGTGTAGTTGCGTAAATGGGCAAAAGTAATAAGAAACAGGAGGATTCTAAGTTGTGAGGATTTTTCGTTAACTTTACCGCCa                                                                                                                                                                                                                       <  2:586948/229‑1 (MQ=255)
                        tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAAATTTTTCAGAAAAAAAAAAAAAAATGAGGTTGCATGATTAGGGAAAGTAATAAACAACACACAGAGAAAAAAGTTACAGAGATTTTTTCTTACCTTTCCGCCATGAttttcta                                                                                                                                                                                                             >  2:60242/1‑234 (MQ=255)
                         gCCATCCCCTGCCCTGGCGCAATATTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGTTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAAAAAAACGGGAGTTGCAATGATGAGAGAAAGTAATAAGAAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAATTTTACCGCCATGATTTCTAccc                                                                                                                                                                                                           <  1:1142186/239‑1 (MQ=255)
                             tcccttGCTCTGGCGCATTATTCAGTCTTATTTTCGCGTTGATTCACGACCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCATATTTTTAGTGGTTTGTTTTGGCGGTGATTTTTTCAAGAAAAATACAAAACTGGGAGTTGCCATGAGGTGCAAAATTAATAAGCAACAGGAAGTGAATAAGTGGACAGAGATTTCCGATAACTTTACCGCCATGATTTCTACCCGAAt                                                                                                                                                                                                       <  2:1106716/234‑1 (MQ=255)
                               cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAAATGGAGGTGACATTATTGGCGGAAGGAATAAGGAAACAGCAGGGAATAAGTTGAGAAAGGTTGTTGCCTACTTTATCGCCCCGAttttttacccaatg                                                                                                                                                                                                      >  1:1109367/1‑227 (MQ=255)
                                ccggCTCTGGCTAAAATTTCATTCTTATTTCCGATTTAATTAACGAAGTTTTAATTAAATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTATTTTGTGGTGTTAAATTTTTCAAGACAAATAAAAAAACGGGAGTTGCCATGATGGGCTAAAGTAATAAGCAACAGCCATTGAATAAGTTAACAGGGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGcc                                                                                                                                                                                                    <  2:497370/236‑1 (MQ=255)
                                               tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                     <  1:943734/239‑1 (MQ=255)
                                                   cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAATACAAAAAAAAGAGGTGCCATGATGAGCGAAAGGAATAAAAAACAGGCAGAGAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCAATATTTCTAACCCAATGCCTGATGACGCGGGGGGTaaa                                                                                                                                                                                 >  1:719784/1‑239 (MQ=255)
                                                        ttATTTTCGCGTTGATTCACGAACTTTTAATTACATATTCACGGTGGAAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                            <  1:60242/239‑1 (MQ=255)
                                                        ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGAAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTCCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                                                                                                            <  1:353221/239‑1 (MQ=255)
                                                         tATTTTCGCGTTGATTCACGAATTTTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTTTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                           <  1:87828/239‑1 (MQ=255)
                                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGGTGGCATGATGAGCGGAAGGAATAAGCAAAAAGGAGAGAATAAGATGAAAGAGATTTTGGCTAAATTTTACGCCCTGATTTCTTACCCAAAGCCTGAGTAAGGGGGGGGAAAACTaaaa                                                                                                                                                                           >  1:964194/1‑239 (MQ=255)
                                                         tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAAGCAAAAATAAAAACTGGAGTTGCCGTGATGGGGGAAAGGAATAAACAAAAAGAAGGGAAATAAATGGAAGAAATTTTCGCTTAATTTACCCCCCTGATTTTTTCCCGGAAGACTGATTACGTTGGGGGTAAACAaaaa                                                                                                                                                                           >  1:142788/1‑239 (MQ=255)
                                                         aatttcggtcttGCTTCCCAAATTGTAAATTACATTTTCCGGGTGGAAAAAATAAAAAAACCACTTTTTTAGGGGTAGTTTGGGGCGGTGAATTTTTCCAGACAAATAAAAAACCGGGAGTTGCCATGATGAGCGAAATTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                                                                                                           <  2:780063/229‑1 (MQ=255)
                                                                    tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa                                                                                                                                                                                     <  1:57911/218‑1 (MQ=255)
                                                                    tGATTCACAAACTGTTAATTACATATTCACGGTGGAAAAAAAAAAAAAACCACATTTTTAGTGTTATTTTGTGGCGGTAATTTTTTCCAAACAAATACAAAAACTGGAGTTGCCATAATGAGCAAAAGTAATAAGCAACGGGGAGTAAATAAGTTAACAGAGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGa                                                                                                                                                                <  1:193005/239‑1 (MQ=255)
                                                                           caaaCTGTGAATTACATATTCACGGCGGAAAAAAAAAAAAAACCACATTTTTAGTGGTAGTTGGTGGCGGGGAATTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAAACAACAGGCAGTAAATAGGTCGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGaa                                                                                                                                                         <  1:859525/237‑1 (MQ=255)
                                                                              aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAATGGAGTTTCCCTTGTGGGCGGAAGAAAAAAAAAAAAAGAAAAGAATAAAATGACAGAGATTGTCGCTAACTTTTCACCCCAGATTTCTTACCGAATACCCGATGACGTGGTGGGTTAACAAAAAcctgtctcttttacaaatctg                                                                                                                                                      >  2:57911/1‑219 (MQ=255)
                                                                               cttttAATTGCATTTTCCGGGTGGAAAAAAAAAAAAACCCACATTTTGTGGGGTAGTGTGGGGGGGTGATTTTTTCCAGAAAAAAAAAAAAACGTGAGTGGCCATGAGGAGCGAAAGTAATAAGCAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgt                                                                                                                                                     <  2:906499/236‑1 (MQ=255)
                                                                                 gTTAATTACATATTCACGGGAAAAAAAAATATAAAACCACATTTTTGTGGGTAGTTGTTGGCGGTTAATTTTTCAAAACAAATACAAAAACTGGAGTTGCCAGGTGGAGCAAAAATAAAAAGCACTAGGCGTTAAATAAGTGGAAAGAGATTGTCGTTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcgtcg                                                                                                                                                   <  2:964194/239‑1 (MQ=255)
                                                                                  ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAACAAGCAGTGAATAAAGTTACAGAGATTGTCGCTAACTTTACCGCCATGATTTTTTACCGAATGCATGTTGACGTGGTGGGTAAAATAAAAAAACTAAAAGATGGCGCAAcgtcg                                                                                                                                                   >  1:925507/1‑238 (MQ=255)
                                                                                    aaatatcatttcgaggtgaaaaaaaaTATAAAACCCACTTTTAAGTGGTAGTTTGTGGTTGTGAATTTTACAAGACAAAAACAAAAACGGGTGTTGCCATGATTAGCGAAAGTAATAAGCAACAGGTAGTGAATAATTAGACAGAGTTTGTCGAAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtct                                                                                                                                                <  2:997264/219‑2 (MQ=255)
                                                                                    aaTGACATGTTCCCGGTGGAAAAAAATAAAAAACCACATTTTAGGGTGTAGTTTGGGACGGTGAATTTTCCCAAAAAAATAAAAAAACTGGAGTTCCAGTAATGCGCAAAAGTAAAAACAAACGGGCAGTAAATAATTGGACAGAGTTTGCCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                                                <  2:142788/239‑1 (MQ=255)
                                                                                      ttACATATTCGCGGGGGAAAAAAATAAAAAACCACATTTGTATTGGTGTTTTGTGGCGGTGAATTTTTCCAAAAAAAAAAAAAACCGGGAGTTTCAATGAAGAGAAAAAGTAAAAACAAAGGGGCAGTAAATAATGGGAAAGAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGtcgtcg                                                                                                                                                <  2:872854/237‑1 (MQ=255)
                                                                                          atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAATAAAAAAATGAGTTGTCATTATGGGAGAAAATATAAAAAAACAGGAAGTGAAAAAAGTGACAGAGATTGTCGCTAACTTTACACCCCAGATTTTTACCCACACACCTGTTGACATGGGGGGTGAAATCAAAAAGCTAAAAGATGCGGGAAACTCCTAGATgggg                                                                                                                                         >  2:392203/1‑239 (MQ=255)
                                                                                           tatTCAGGGTGGAAAAAAAAATAAACCCATTTTTTGAGTGGTGGTTTGGGGCGGTCAATTTTGCCAAAAAAAAAAAAAAACGGGAGTTACAATGATGGGAGAAAGTAATAAGCAAGAGGCACTGAAAGCGTGGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                        <  1:827442/239‑1 (MQ=255)
                                                                                           tatTCACGGTGGCAAAAAATATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGATTTTTACCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGAGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                        <  1:662820/239‑1 (MQ=255)
                                                                                           tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                        <  1:903447/239‑1 (MQ=255)
                                                                                            atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTCCACAACAAAAAAAAAAAATGGGTTTGCATGATGAGGAGAAAAAATAAAAAACAGGCAGTGAATAAGATGATAGAGAATGTCGCTAACTTTAACGCCATGATTTCTACCCCAATGGCTGGTGACGGGGGGGGTAAACTCAAAAAAATAAAGGGAGCCGAAAAGGAGTCGGTgggggg                                                                                                                                       >  2:183559/1‑237 (MQ=255)
                                                                                             gacACGGTGGCAAAAAAAAAAAAACAAAATTTTTATGGGTAGTGTGTGGGGGTGAATTTTTCAAGCAAAATAAAAAATCTGGAGTTGCAATGAGGAGAGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGTTTGTCGTTAATTTTTCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                                                                                                                        <  2:719784/235‑1 (MQ=255)
                                                                                               cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGAAACAGGCAAGGAATAAGATTACAGAGAATTTCGCTTACTTTACCGCCATGATTTTTACCCGAAAGCCCGATGAGGTGGGGGATAAACTAAAAAAACTAAAAGAAGCCCGAAAGTCGTCGATGGGGGAAAt                                                                                                                                    >  1:871285/1‑239 (MQ=255)
                                                                                                   tggaaaaaaaaTAAAAAAACCATTTGTTGTTGGTGGTGTGTGGGGGTAAATTTTTCGAAACAAAAACAAAAACTGGAGTTACTATGATGGGCGAAATAAATAAGCAACAGGAAGTTAATAAGTTCATACAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACTTCGTCGATGGGGAAAATTAt                                                                                                                                 <  2:174226/233‑1 (MQ=255)
                                                                                                    tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGAAAAAAAAAAAAAATTGAGTTGCCCTGATGAGCGGAAGGAATAAACAAAAGGCCGTGAATAAGTTGACAGAGATTTTCGCTAACTTTACCGCCATGATTTTTTCCCGCAAGCCTGATGAAGGGGGGGGTAAACTAAAAAAGCTAAAGGATGCCGGAAAGTCGTCGCTGGGGGAAATTATCt                                                                                                                               >  1:832264/1‑239 (MQ=255)
                                                                                                      gaaaaaaaaaaaaaCCCACATTTTTAGTGGTAGTTTGGGGCGGTGAATTTTTCAAGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTa                                                                                                                              <  1:1111938/236‑1 (MQ=255)
                                                                                                      gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAACAGGCAGTGAATAAAATTACAGAGATTGTAGCTAACTTTAACGCCATGATTTCTTCCCGCATGCCTGATGACGGGGTGGGTAAAATAAAAAAGCTAAAAGAAGGCGAAACGTCGTCGGTGGGGGAAATtatata                                                                                                                              >  1:1148268/1‑238 (MQ=255)
                                                                                                              tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                                                                                                                          <  1:691234/218‑1 (MQ=255)
                                                                                                                taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCCAAAGCCTGATGACGGGGGGGGTAAAATAAAAAAGCTAAAGGATGCCGAAACGTCGTCGATGGGGGAAAATATTTACCATACCATGt                                                                                                                   >  1:258223/1‑239 (MQ=255)
                                                                                                                        caTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGAAAAAAAAAAAAAATGGAGGTGGCATGTTGAGGGGAAGTAATAAGAAAAAAGAAGTGAAAAAAGTGAAAGAGATTGTGTGTAAATTTTACGCCATGAATTCTACCCGAATACCTTATGAAGTGGTGGGTAAAATAAAAAAGCTAAAGAAAGACGGAACGTCGTCGATGGGGGAAATTAtttttgtactttatacacatctg                                                                                                           >  2:691234/1‑217 (MQ=255)
                                                                                                                                      aGTTTGTGGGGGAGAATTTTTCCAAAAAAATAAAAAAACTGGAGTTGCAATGAGGAACGAAAGAAATAAGCAACAGGCGGTAAAGAAGTGGACAGATATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                              <  2:493407/239‑1 (MQ=255)
                                                                                                                                      aGTTGGTGGAGGTAAATTTGACAACAAAAATACAAAAACTGAAGTTGCCATGATGAGCGAAAGGAATAACAAACAGCCAAGAAATAAGTTGACTGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGa                                                                                              <  2:533339/239‑1 (MQ=255)
                                                                                                                                        tttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATa                                                                                                                         <  1:113038/210‑1 (MQ=255)
                                                                                                                                                        tttCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAAAAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                            <  1:796851/239‑1 (MQ=255)
                                                                                                                                                        gtgccAAACAAAGAAAAAAACTGGAGTTGCCATAATGAGCGAAAGAAAAAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGTAAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTcctg                                                                            <  2:363770/236‑1 (MQ=255)
                                                                                                                                                                           actgggAGTAGCAATTAGGAGCGAAAGAAATAGGCAACAGGAAGGAAATAAGTTGACAGAGATTTCCGCTAATTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                          <  2:756761/235‑1 (MQ=255)
                                                                                                                                                                           aaCGGGAGTTGCCAAGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                          <  1:733907/239‑1 (MQ=255)
                                                                                                                                                                           aaCGGGAGGTGCCATGATGAGCGAAAGTAATAATCAACGGGCAGTAAATAATTTACCAGAGTTTGTCCCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCgggg                                                          <  2:925507/239‑1 (MQ=255)
                                                                                                                                                                                              aGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGAACTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTaa                                       >  1:1074648/1‑239 (MQ=255)
                                                                                                                                                                                                        ataaGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                              >  2:531185/1‑238 (MQ=255)
                                                                                                                                                                                                        ataaGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                              >  1:795015/1‑238 (MQ=255)
                                                                                                                                                                                                        ataaGCAACAGGCAGTAAATAAGTTGACAGAGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTc                              <  1:53372/239‑1 (MQ=255)
                                                                                                                                                                                                          aaGAAACAGGCATGAAATAAGTTGACAGAGATTTTCTCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCg                           <  1:733017/239‑1 (MQ=255)
                                                                                                                                                                                                                cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTc                                                                               >  1:234848/1‑181 (MQ=255)
                                                                                                                                                                                                                cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTccc                     >  1:403995/1‑239 (MQ=255)
                                                                                                                                                                                                                cAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTcc                      >  1:560259/1‑238 (MQ=255)
                                                                                                                                                                                                                     cggtaaaTAAGTTGACAGAGATTGTCTCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCa                    <  2:258223/231‑1 (MQ=255)
                                                                                                                                                                                                                     aGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGCATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACt                  >  2:889578/1‑237 (MQ=255)
                                                                                                                                                                                                                       tGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGGTAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCtt              >  2:925485/1‑239 (MQ=255)
                                                                                                                                                                                                                       tGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCtt              >  2:1010254/1‑239 (MQ=255)
                                                                                                                                                                                                                            taattacgcAGAGATTGCCCATATGTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg          <  2:1148268/231‑1 (MQ=255)
                                                                                                                                                                                                                            tAAGTTGAAAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCg          <  1:531185/239‑1 (MQ=255)
                                                                                                                                                                                                                              aGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAg        <  1:1014962/239‑1 (MQ=255)
                                                                                                                                                                                                                              aGTTGAAAGAGATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAg        <  1:391834/239‑1 (MQ=255)
                                                                                                                                                                                                                               gTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGtt                                                >  1:661725/1‑198 (MQ=255)
                                                                                                                                                                                                                                  gACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc    >  1:251514/1‑239 (MQ=255)
                                                                                                                                                                                                                                  gACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGc    >  2:1082443/1‑239 (MQ=255)
                                                                                                                                                                                                                                    cAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGAACACCGGGGAGATTATGATCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCaa  >  2:845106/1‑239 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               
AGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATTGACCTGAATCGTCCTGCCTGTCAGGACACCGGGGAGATTATGTTCTTCGTTAAAGTCGGTTCCCGCTTCCCACTGCTTGGCGAGCTGCAA  >  NC_000913/3206279‑3206742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: