Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,336,882 T→A 13.3% I51F (ATC→TTC)  adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,8820TA13.3% 187.8 / 3.5 76I51F (ATC→TTC) adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base T (48/17);  new base A (2/9);  total (50/26)
Fisher's exact test for biased strand distribution p-value = 7.16e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

ATGGTTAATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAC  >  NC_000913/4336653‑4337117
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
aTGGTTAATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCac                                                                                                                                                                                                                                     <  2:466752/239‑1 (MQ=255)
  ggTTAATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCacca                                                                                                                                                                                                                                   <  1:985701/239‑1 (MQ=255)
  ggTTAATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCacca                                                                                                                                                                                                                                   >  1:367107/1‑239 (MQ=255)
       aTACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCaccaacca                                                                                                                                                                                                                               >  1:85428/1‑238 (MQ=255)
       aTACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAAccat                                                                                                                                                                                                                              <  1:812714/239‑1 (MQ=255)
            aaTGGATCTTTCAGAATCGGGACGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCCGCCAGCAGACGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCCTAAGCGTAAGAACCACCACGCCTTGGGTCGGGGAACCACATTTTCGGGTTTACAAAAGAGACCCCCAGCGCACCGaaaagcctcccccacaattccta                                                                                                                                                                                                                         >  2:82503/1‑220 (MQ=255)
              tGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGCAAGCCCCAGTGCACCGATAATCGTCACCAAACATCCATaaa                                                                                                                                                                                                                       >  1:771709/1‑239 (MQ=255)
              tGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATaaa                                                                                                                                                                                                                       >  1:587356/1‑239 (MQ=255)
              tGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATaaa                                                                                                                                                                                                                       >  1:302950/1‑239 (MQ=255)
              tGGATCTTTCAGAATCGGGAACAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTACCGATCCAGCAGGCCAGCCAGTAGAGGACGTTAGTTTGATAACCGAGAAACGGGCCATAGCAGCGGCGGGCGTTAACGTAAAAAACACCAACGCTTGGGGCCAAGCACAGCAATTTGCCGTAATACAATCAGAGCCCCAGTGGCCCGGTAATCGGAaacaaccaatcaataa                                                                                                                                                                                                                       >  2:297415/1‑231 (MQ=255)
                     ttCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCaa                                                                                                                                                                                                                 >  1:646462/1‑238 (MQ=255)
                          aaTCGGGAAGAAGTAACATAAAGATCCTCCGCAAAGGACCACGATGGGGATCAGGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTGGTTTTGATAACCGAAAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGAAAAACCCACCAGGACTGGGGTCGAGGACCGAATTTTTGGCGTATACCATCGAGCCCCCCAGTGCACCAATAATCGTCACCACCCACCCATAAATGGCAATCCCGc                                                                                                                                                                                                          <  2:367107/239‑1 (MQ=255)
                                    aagTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAAACGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAgg                                                                                                                                                                                                 >  2:685488/1‑238 (MQ=255)
                                         aCTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGt                                                                                                                                                                                           >  1:381832/1‑239 (MQ=255)
                                          cTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGGATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGtt                                                                                                                                                                                          >  1:656048/1‑239 (MQ=255)
                                              aaTATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCa                                                                                                                                                                                      >  1:272150/1‑239 (MQ=255)
                                              aaTATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGCAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCCGGCCTTTGGTCGAGGAACCACATTTTGGCGGATACCATCCAGAGCCCCAGTGCACCGATCATCGTCACCACCCATCCCTAAATGGCAATCCCGCCAGTAACGACCATGGTTGCa                                                                                                                                                                                      >  2:1031455/1‑239 (MQ=255)
                                                     tACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCa                                                                                                                                                                                      >  1:376209/1‑232 (MQ=255)
                                                     tACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACa                                                                                                                                                                               >  1:616477/1‑239 (MQ=255)
                                                     tACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACa                                                                                                                                                                               >  1:3991/1‑239 (MQ=255)
                                                     tACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACa                                                                                                                                                                               >  1:163052/1‑239 (MQ=255)
                                                       cGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGACCAGGTTTGCAGGTAACAGa                                                                                                                                                                             >  1:809139/1‑239 (MQ=255)
                                                       cGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAAACCCGCCCGGAGAGGCACGGGTTGAAGGTAACAGa                                                                                                                                                                             >  2:363110/1‑239 (MQ=255)
                                                       cGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAAACATCCATAAATGGCAATCCCGCCAGTAAAGGGCACGTTTGCAGGGAACAGa                                                                                                                                                                             >  2:282772/1‑239 (MQ=255)
                                                       cGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCAACAACCATCCATAAATGGCAATCCCGCCAGTAAGGGCCAGGTTTGCAGGTAACAGa                                                                                                                                                                             >  1:691482/1‑239 (MQ=255)
                                                       cGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCAAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGAAATTTTTGCGTATACCATCGAGAGCCCCAGTGCACCGGTAATCGTCATTAACCATCCATAAATGGCAATCCCGCCAGTAGCAGCCAGGTTTCAACGTAACAGa                                                                                                                                                                             >  2:43177/1‑239 (MQ=255)
                                                            aTGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCCGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGGAACAGaaaaaa                                                                                                                                                                        >  2:396617/1‑238 (MQ=255)
                                                            aTGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAac                                                                                                                                                                        >  1:318591/1‑239 (MQ=255)
                                                            aTGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAAACACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCAACGAGAGCCCCAAGGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGCCCAACATTGCAGGGAAccacaaata                                                                                                                                                                        >  2:915069/1‑231 (MQ=255)
                                                                       ggCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCa                                                                                                                                                             >  1:989347/1‑239 (MQ=255)
                                                                        ggtatatCGCCGGTCCAGCAGGCCAGCCAGTAGAGGCCGTTGGTTTGAAACCCGAGAAACGGCCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCTTATCCCAGCGAGAGCCCCAGTGCACCGAAAATCGTCACCAACCACCCATAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGAccc                                                                                                                                                               <  1:1094381/233‑1 (MQ=255)
                                                                              ttGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTTGGACGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATGGTCACCAACCCTCCATAAATGCCAATCCCGCCAGTAGAGGCACGGTCTGCCAGTAACAGAAAAACACCTGCACCCCTAatat                                                                                                                                                       >  2:597601/1‑238 (MQ=255)
                                                                                               cagccagAAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGAGTAAGCGTAAGAACAGCCAGGTCGTGGGACGAGGAAAGACGTTTTGGCGCATACCACCGAGAGCCCCCGGGCACCGGAAATAGTCACGCGCCAACCATTAAAGGCAATCCCGCCAGTAAAGGCCAGGTTGTCAAGGAACAAAAAAACAACCCACCCCACAAGATTACCCGACACCacctgtg                                                                                                                                     >  2:368597/1‑233 (MQ=255)
                                                                                                           gACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGGAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTa                                                                                                                         >  1:588020/1‑239 (MQ=255)
                                                                                                              gTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                                                                                       >  1:361375/1‑238 (MQ=255)
                                                                                                               ttGGTTTGATACCCGAGAAACGGCCGAAACCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGCCTGGGCTCGAGAAACGACATTTGGCCGAATACCACGGAGAGCCCCAGGGCACCGAAAATCGTCACCACCAACCCAAAAATGTCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGcc                                                                                                                     <  2:302950/239‑1 (MQ=255)
                                                                                                                tGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGccc                                                                                                                    >  1:663446/1‑239 (MQ=255)
                                                                                                                tGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGGAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCCAGAGCCCCAGCGCACCGATAAACGTCACCAACCATACATAAATGGCAAACCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGTAGGGGAATTAGccc                                                                                                                    >  2:809281/1‑239 (MQ=255)
                                                                                                                       aTAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgt                                                                                                             >  2:281210/1‑239 (MQ=255)
                                                                                                                                 aaCGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagc                                                                                                   >  1:913600/1‑239 (MQ=255)
                                                                                                                                          aaGCGGCGGGGGGCGTAAGCGTAAGAACCCCAAGGACTTGGGTGGAGGAACGACATTTGGGCGTATCCCACCGAGAGCCCCAGTGCACGGAAAATCGTCACCAACCATCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGa                                                                                          <  1:685488/239‑1 (MQ=255)
                                                                                                                                              agcGGCGCGCGAAAGCTAAACAACCACAGGCACTGGGGCCGAGGAACAACTATGTGCCATAACCCATCGAAACCCCCGCTGCACCGAAAATCGTCACAACCAACCCAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACAt                                                                                       <  2:618999/238‑1 (MQ=255)
                                                                                                                                                cggcggGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAt                                                                                    >  1:468697/1‑239 (MQ=255)
                                                                                                                                                cggcggGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAt                                                                                    >  1:79805/1‑239 (MQ=255)
                                                                                                                                                cggcggGCGTAAGCGTAAGAACAACCAGGACTTGGGTCGAGGAACGACATTTTGGCGGATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAAAAGCAATCCCGCCAGTAGAGGCCAGGTATGACGGTAACAGAAAAACACCTGACCCCATAATAATACCCGAACACATCAGGGCGACGGGGATTAAGCCCACTTTTTGAGCCACAGACTCCGCAGACATAAt                                                                                    >  2:682460/1‑239 (MQ=255)
                                                                                                                                                   cgggcgTAAGCGTAAGAACCACCAGGTCTTGGGTCGAGAAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCACCCACCCATAAATGCCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa                                                                                  <  1:363110/238‑1 (MQ=255)
                                                                                                                                                      gcgtaagcgtaaGAACCCCCAGGACTTGGGTCGAGGAACGACTTTTGGGCGTATCCCATGGAGAGCCCCAGTGCACCGATAATCGTCACCAACCACCAAAAAAGGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAAc                                                                                                                                                                                <  1:398525/141‑1 (MQ=255)
                                                                                                                                                       cgataacgtaaagcgccacaagtcTTGGGTGGAGGATACGACATTAGGCCCTATACCTGCGAGGGCCCCAGGGCACCGAAAAACGCCCCAACCCACCCAAAAATGGCATGCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAAc                                                                               <  2:303792/215‑1 (MQ=255)
                                                                                                                                                                aagAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAacac                                                                                                                                                                      >  2:398525/1‑141 (MQ=255)
                                                                                                                                                                        ccaGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAACCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGCCATCATTAAACTCCTGCGAAGGCGAGCTTc                                                            >  1:388373/1‑239 (MQ=255)
                                                                                                                                                                        ccaGGACTTGGGTAGCGGAACGACATTTTGGCGTATACCACCGCGAGACCCAGTGCACCGAAAATCGTCACCAACCATCAATAAATGGCAATCCCGCCAGTAGATGCCAGGGGTGCAGGAAACAGAAAAGCACCTGACCCCATAATATTCCCCGACACCATCAGGGCGACGGGGATTAAGCCAACTTTGTGAGCATCAGCAACCGAAGACGTAATTAAACACCTGCGCAGTCGTGCtac                                                            >  2:284116/1‑237 (MQ=255)
                                                                                                                                                                         caGGACTTGGGTCGAGGCACGCCATTTTGGCGTATACCATCGAGAGCCCCCGTGCACCGATAATCGTCACCACCCATCCATAAATGGCAATCCCGCCAGTAGAGGCCGGGTTTGCAGGTAACAGAAAAACCCCTGACCCCCTAATATTCCCCGACACCATCAGGGTGCCGGGGCTTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACCTAATTAAACTCCTGCGACGGCGCGCtta                                                            >  2:554764/1‑237 (MQ=255)
                                                                                                                                                                                 gggTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATa                                                   >  1:589199/1‑239 (MQ=255)
                                                                                                                                                                                       aGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                             >  1:422417/1‑239 (MQ=255)
                                                                                                                                                                                          aaCGACATTTTGGCTAATACCACCAAGAGCCCCAGTGCACCGAAAACCGCCACAACCCATCAATAAATGGAAATACCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGt                                           <  1:282772/238‑1 (MQ=255)
                                                                                                                                                                                            aCGACATTTTGGGGTATCCCAGCAGGAGCCCCAGTGCCCCGATAACCGTCCCCAACCACCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtca                                         <  1:1031455/239‑1 (MQ=255)
                                                                                                                                                                                            aCGAAATTTTGGCGTATGCCATCGAGAACCCAATTGACCCGATAAGCCTCACCAACCATCCATAAATGCCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtca                                         <  1:152884/239‑1 (MQ=255)
                                                                                                                                                                                                cATTTTGGCGTATCCCAGCGAGAGCCCAAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatat                                      <  1:43177/238‑1 (MQ=255)
                                                                                                                                                                                                       gCGTATCCCATCGAGACCCCCAGTGCACCGATAATCGCCACCAACCATCAAAAAATGGCACTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGc                               <  2:163052/238‑1 (MQ=255)
                                                                                                                                                                                                         cctatcccaccggaaggcccAGTGCCCCCTAAATCCACCCCAACCAACCAAAAAAGGCAACCCCCGCCAGTAGAGGCCAGGTTTGCAGTTAACAGAAAAACCCCTGCCCCCATAATATTCCCCAACACCATCAGGGTGCCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGc                               <  1:460973/221‑1 (MQ=255)
                                                                                                                                                                                                          tataCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGc                           >  1:904189/1‑239 (MQ=255)
                                                                                                                                                                                                             taCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAt                         >  2:277742/1‑239 (MQ=255)
                                                                                                                                                                                                                 atggagaGCCCCTGTGCCCCGAAAACTCCCACCAACAATCAAAAAATGGCAACTCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                     <  1:1108221/236‑1 (MQ=255)
                                                                                                                                                                                                                 aTCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                     <  1:396617/239‑1 (MQ=255)
                                                                                                                                                                                                                  tCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAt                    >  2:567466/1‑239 (MQ=255)
                                                                                                                                                                                                                  tCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAt                    >  1:529753/1‑239 (MQ=255)
                                                                                                                                                                                                                    taggtccccgttcaaCCGATAACCGTCACAAACCATCGAAAAAGGTCAACTCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                  <  1:158752/225‑1 (MQ=255)
                                                                                                                                                                                                                         ccccAGTGCCCCGAAAATCGGCAACAACCACACAAAAATGGAAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGaa             <  2:904189/239‑1 (MQ=255)
                                                                                                                                                                                                                          cccAGTGCACGGATAATCGCCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCt                                                                                                                                                                    <  1:932025/87‑1 (MQ=255)
                                                                                                                                                                                                                          cccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCt                                                                                                                                                                    >  2:932025/1‑87 (MQ=255)
                                                                                                                                                                                                                          cccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAat            >  2:695104/1‑239 (MQ=255)
                                                                                                                                                                                                                             aGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGa                     >  1:470078/1‑227 (MQ=255)
                                                                                                                                                                                                                               cgccacaaTAATCACCACCAACAATCAAAAAACGACAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattatt        <  2:910914/231‑1 (MQ=255)
                                                                                                                                                                                                                                  aCCGATAATCCTCACCACCCACCCAAAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATc     <  1:597601/238‑1 (MQ=255)
                                                                                                                                                                                                                                    gtaTAATCGCCCCAAACCAACCATAAGGGGAATCCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAc  <  2:529753/237‑1 (MQ=255)
                                                                                                                                                                                                                                    cGATAACCGTCACCAACCAACAAAAAAGGGCAACCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGa                                                                                          <  2:910858/151‑1 (MQ=255)
                                                                                                                                                                                                                                      aTAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatcata                                  >  1:13687/1‑205 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
ATGGTTAATACCAATGGATCTTTCAGAATCGGGAAGAAGTAACTTAAATATCCTACGCCAATGACCACCATGGCGATATTGCCGATCCAGCAGGCCAGCCAGTAGAGGACGTTGGTTTGATAACCGAGAAACGGGCCAAAGCAGCGGCGGGCGTAAGCGTAAGAACCACCAGGACTTGGGTCGAGGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGAC  >  NC_000913/4336653‑4337117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: