Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	530,732	A→C	19.9%	G165G (GGT→GGG)	mnmH ←	tRNA 2‑selenouridine synthase, selenophosphate‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	530,732	0	A	C	19.9%	168.4 / 4.8	85	G165G (GGT→GGG)	mnmH	tRNA 2‑selenouridine synthase, selenophosphate‑dependent
Reads supporting (aligned to +/- strand):  ref base A (31/30);  new base C (2/17);  total (33/52)
Fisher's exact test for biased strand distribution p-value = 2.65e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCAC  >  NC_000913/530495‑530969                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 aTCTTCTACCACCGAAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGACTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGGCAGCAGGTTGTCAAAAATCGCCTGGCTAAGTT‑GTTGTTGTAACGAGCGACCAAACGCCGAA‑CCGCGAGGACGAGCCAACCCTTCCAGATCAaca                                                                                                                                                                                                                                                  >  2:1039089/1‑238 (MQ=255)    ttatcgcactGCAATCGCCGCCCTGCTCACATGCTCGCGCCGGCATTCCGGCCGGTCATTCCAACCGATCATCCGGCTTTCGTCTTCCAGCACCCTCAGTCGCCAATTCTGACGGGCATCGGTTTTTTGCATTTCGGCAGCCAGCATGTTATCAAAACTCGCCTGGCCAAGTT‑CTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGTCGCGCCAACCCTTCCAGATCAACACCG‑t                                                                                                                                                                                                                                             <  2:194456/233‑1 (MQ=255)         ccaccGCATCCGCCGACTGAGACATTCGCTCGCGCAGGCAGTCCGGCAGGTGATTCTAACCGAACCACCGGCTTTCGTCTTCTCGCACCCACAGGCGCAACTTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGACAGCAGGTTTTCAAAACTCGCATGGCTAAGTT‑GTGGTTGTAACGTGCGAACAAACGCCGCA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACCCCG‑TT‑CGGc                                                                                                                                                                                                                                       <  2:1071502/239‑1 (MQ=255)             cGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  2:270583/1‑239 (MQ=255)             cGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  1:509973/1‑239 (MQ=255)             cGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  1:852776/1‑239 (MQ=255)             cGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  1:863568/1‑239 (MQ=255)             cGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  1:131271/1‑239 (MQ=255)             cGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGATTTCATCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGGCAGCAGGTCTTCAAAACTCGCCTGGATACGTT‑GCGGTTGTAACACGCGCCCAACCGCCGAA‑CCGCGATGACGTCCCAACCATTCAATATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  2:638326/1‑239 (MQ=255)              gCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCCAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTCtt                                                                                                                                                                                                                                   >  2:163188/1‑238 (MQ=255)               cAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  1:709864/1‑237 (MQ=255)               cAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  1:583651/1‑237 (MQ=255)               cAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAAATCAACCCCG‑TT‑CGGCTGtt                                                                                                                                                                                                                                   >  1:774201/1‑237 (MQ=255)                aaTCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTtgc                                                                                                                                                                                                                                 <  2:863568/238‑1 (MQ=255)                aaTCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTtgc                                                                                                                                                                                                                                 <  2:231489/238‑1 (MQ=255)                    gccgccTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACCCCG‑TT‑CGGCTGTTGCTGCAc                                                                                                                                                                                                                            >  2:2048/1‑239 (MQ=255)                       gccTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTaa                                                                                                                                                                                                                         <  2:471692/239‑1 (MQ=255)                         cTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACa                                                                                                                                                                                                                       <  1:270583/239‑1 (MQ=255)                                 ttCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑a                                                                                                                                                                                                              <  1:517328/239‑1 (MQ=255)                                   cgctcgcGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑Acc                                                                                                                                                                                                            <  1:450425/239‑1 (MQ=255)                                   cgctcgcGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑Acc                                                                                                                                                                                                            <  2:888262/239‑1 (MQ=255)                                      tcgcGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑Acc                                                                                                                                                                                                            >  2:67256/1‑236 (MQ=255)                                                       cagtggATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCCCAGGCGCAAATTCTGACGGTGGTCGGTTTTTAGCATTGCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCACCCCTTCCAGATCAACCCCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCa                                                                                                                                                                                         <  2:536350/233‑1 (MQ=255)                                                       cAGGTGATTCGACCCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCACCCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCa                                                                                                                                                                                         <  1:889289/238‑1 (MQ=255)                                                        aGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCACATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCATTAACCGCGTTTT‑ACCGCTGCCGGGACACCCGCCaa                                                                                                                                                                                        >  2:916669/1‑238 (MQ=255)                                                         gctGATTCGAACCGTTAATCCGGCTTTCGTCGTCAAGCGCCCACGATGGCAAATGCTGCCGGGCGTCGGTTGGTAGGATTCCGGCAACAGGAAGCTTTTAAAATCTCGTCGGGCTAAGTG‑GTGGTTGTAACGTGGGACCAAACGCGGAA‑CCGCGATGCCCCGCCAACCCTCCAAGATCAACACCT‑TT‑CGGCTTTGGCTGCACTAACAGCGTTTT‑ACCGCTGCCGTTCCACCCGCCAATc                                                                                                                                                                                      <  2:668744/237‑1 (MQ=255)                                                          gTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGCGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACATCGTTTT‑ACCGCTGCCGGTACAACCGCCCATCa                                                                                                                                                                                     >  2:245939/1‑239 (MQ=255)                                                            gATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCCACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGCTTT‑ACCGCTGCCGGTACAACCGCCAATCCGc                                                                                                                                                                                   >  2:248313/1‑239 (MQ=255)                                                            gATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAg                                                                                                                                                                                    <  1:918917/238‑1 (MQ=255)                                                                 gAACCGATCATCCGGCTTTCGTCTTCCGGCACCCACAGGCGCAAATTCTGCCGGGCGTCGGTTTTTAGCTTTTCGGCACCCAGCAGGTTTTCAAAACTCGCCTGTCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCACCCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTAt                                                                                                                                                                              <  2:605172/239‑1 (MQ=255)                                                                   aCCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCg                                                                                                                                                                            >  1:796622/1‑239 (MQ=255)                                                                       atcatcCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGttt                                                                                                                                                                        >  1:1065157/1‑239 (MQ=255)                                                                            ccGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTtgt                                                                                                                                                                    >  1:875172/1‑238 (MQ=255)                                                                             cgtctttctttTTCCAGCCCCCACAGGCGAAAATCCTGAGGGTCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GGGGTTGTAACGTGCGACAAAACCCCGAA‑CCGCGATGACGCGCCACCCCTTCCAGATCACCCCCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGc                                                                                                                                                                  <  2:509973/236‑1 (MQ=255)                                                                             cggtgttCGTTTGCCAGCCCGCACAGGCGCAAATCGTGACGGGCGTGTGTTTTTACCATTGCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGTTAAGTT‑GGGGTTGTAACGTGCGACCAACCGCCGAA‑CCGCGAGGACGCGCCACCCCTCCCAGATCAACACCT‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGc                                                                                                                                                                  <  2:625742/234‑1 (MQ=255)                                                                             cGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGc                                                                                                                                                                  <  1:67256/239‑1 (MQ=255)                                                                              ggCTTCCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGTTTTTCAAACCTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCACCCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGcc                                                                                                                                                                 <  1:32170/239‑1 (MQ=255)                                                                                          ccAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTc                                                                                                                                                     >  1:393666/1‑239 (MQ=255)                                                                                                    accgcccAAATGCTGGCGGGCGTCAGTTTTTAGCCTTTCGGCAGCCAGCAGGTTTTCAAACCGCGCCTGGCTAAGTT‑GTGGTTGTAACGGGCCACCAAACGCCGAA‑GCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACGGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGGCGCCTGAATcg                                                                                                                                           <  2:1022245/233‑1 (MQ=255)                                                                                                        cgcAAATTCGGGCGGGCGTCGGTTTTGAGCATTGCGGCAGCCAGCAGGTTTCCAAAACTCGCCTGGTTAAGTG‑GTGTTTGTAACGTGCGACCAAACGCCGAC‑CCGCGATGACGCCCCACCCCTTCCAGACCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑CCCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATcg                                                                                                                                           <  2:1063973/235‑1 (MQ=255)                                                                                                           aaaTTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTg                                                                                                                                    >  2:1001838/1‑239 (MQ=255)                                                                                                              ttgtGACGGGCGTCGGTTTTTAGCATTCCGGGAGCCAGCAGGTTTTCAAAACGCGCTGGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAC‑CCGCGATGACGCGCCAACCCTTCCAGATCACCCCCT‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTCCAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGgcg                                                                                                                                 <  2:416720/236‑1 (MQ=255)                                                                                                              ttCGGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCTGCAGGTTTTCAAAACCCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGAGGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGgcg                                                                                                                                 <  1:638326/239‑1 (MQ=255)                                                                                                                tggACGGGCGTCGTTTTTTAGAATTCGGGCAGCCAGCAGGTTTTCAAACCTCGCCTGGCTAAGTT‑TTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCTACCCCTTCCAGACCAACCCCG‑TT‑CGGCTGTTGCGGCACAAACAGCTTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCa                                                                                                                               <  2:1015712/237‑1 (MQ=255)                                                                                                                cTGACCGGCGTCGGTTATTAGTATTTCGGCAACCAGCCGGTTTAGAAAACTCGCCTGGCTATGTA‑GGGTTTGTAACGTGCACTCCACCCCCGCC‑CCGCGAGGACGCGCCAACCCTCCCAGATCAACCCCG‑TC‑CGGCTGTTTCTGCACTAACAGCGTTTT‑ACCGCGGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCa                                                                                                                               <  2:1096207/239‑1 (MQ=255)                                                                                                                 tgcacggcccgggTTTTGAGCATTGCGGCGGCCCGCGAGTTTCAAAAGCTCGCCAGAGTAAGTT‑TTGGTTGTAACTCGCGACCAACCGCCTAA‑CCGCGTTTACCCGCTGCCCTTTCCAGATCTCCACCG‑TT‑GGGCTGTTGTTAAACTAAAAACTTTTT‑ACCGCTTCCTGTACATCCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAg                                                                                                                              <  2:216245/228‑1 (MQ=255)                                                                                                                 tGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAg                                                                                                                              <  1:2048/239‑1 (MQ=255)                                                                                                                   aCGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGt                                                                                                                             >  1:357694/1‑238 (MQ=255)                                                                                                                        cGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGc                                                                                                                                                                  <  2:106138/196‑1 (MQ=255)                                                                                                                        cGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGc                                                                                                                                                                  >  1:106138/1‑196 (MQ=255)                                                                                                                        cGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTta                                                                                                                       >  1:220441/1‑239 (MQ=255)                                                                                                                        cGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTta                                                                                                                       >  1:467490/1‑239 (MQ=255)                                                                                                                             gTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGCACAACCGCCAATCAGCACTATTGGCTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAAc                                                                                                                   >  1:764442/1‑238 (MQ=255)                                                                                                                                      aTTTGGCCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAATGT‑GTGTTGGTAACGTGCGTCCAACCGCCGAA‑CCGCGATGCCGCGCCACCCCTTCCAGATCACCACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTccac                                                                                                         <  2:584838/239‑1 (MQ=255)                                                                                                                                            gCAGCCGGCGGTGTTCCAACACTCGCCT‑GCTAAATTCGTGGTTGTAACGTGCCCCCAACCCCCGAC‑CCGCATTGCCCCCCCACCCTTTCCAACTCCGCCCCG‑TT‑CGGCTGTTGCTGCCCTAACGGCTTTTT‑CCCGCTGCCTGTACACCCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCg                                                                                                    <  2:583651/238‑1 (MQ=255)                                                                                                                                              acccagcATGTTTCCAAACCGCGCCTGGCTAAGTT‑GGGGTTGTACCGTGCGCCCAAACGCCGAA‑CCGCGATGCCGCCCCAACCCTTCCAGATCAACCCCG‑TT‑CGGCTTTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGa                                                                                                  <  2:709864/236‑1 (MQ=255)                                                                                                                                                     agGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGAGGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCATTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatc                                                                                             <  1:916669/236‑1 (MQ=255)                                                                                                                                                     agGTTGTCAAAACGCGCTTGGCTAAGTG‑TGGGTGGTAACGTGCGACC‑AACCCCGAACCCGCGATGCCGCCCCAACCCTTCCAGACCAACCCCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaatc                                                                                             <  2:519252/236‑1 (MQ=255)                                                                                                                                                                    gCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgcatgca                                                                            >  2:179779/1‑238 (MQ=255)                                                                                                                                                                      cgggCTAATTC‑TTGGTGATAAGGTGCCACCAAAGACGGAA‑CCGCTGTGACGCTCCCACCCTCCCAGACCAACGCCG‑TT‑CGGCGGGTGCTGCACAATCAGCGTTTT‑GATGCTGCCGGTACGCCGGCCAATCAGCGCTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCTCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAAc                                                                          <  2:229/236‑1 (MQ=255)                                                                                                                                                                      cggacTAATTG‑GTGGTTGCAACGTGCGACCATAACCCGAG‑CCGCAATTGCGCTCCAAGCCTCCCACAACATCATCG‑TT‑CGGCTTGTCCTTCCCTACGGCCGTTTT‑ACCGCGGCCGGTACAGCCGCCCATCGGCACTATCGGTTTTAGTGCCGTTTCAATAGTCGCCTGAAACGCGGTCTGGCGCAGTGCCTTATATCCGCCTTGCACCAGCGGATAATCAATCCCCGCTGCATGCAAc                                                                          <  2:595327/234‑1 (MQ=255)                                                                                                                                                                      ccggCTAAGTT‑GGGGTTGTAACGTGCGACCAAACGCCGAC‑CCGCGATGACGCGCCAACCCTTCCAGATCAACCCCG‑GT‑CGGCTGTTCCGGAACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAAc                                                                          <  2:1065157/236‑1 (MQ=255)                                                                                                                                                                           tAAGTT‑GTGGTTTACACGGCCGACCAACCACGTAA‑CCGCTATGACCCGCCAGCCGCTCTAAATCAGCACCC‑TG‑CGGCTGTTGCTGCCCTAGCAGCGTTTT‑TCGGCTGCCGGTACAACCGACAATCAGCACTATCGGTTGTTGTGCCAGTTCAATAGTCGCATGAATCGCAGTCTGGCGCATTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATACCCGCTGCATGcagccagcgt                                                                    <  2:964071/239‑3 (MQ=255)                                                                                                                                                                           tAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAGCCCTTCCAGTTCAACACCG‑TT‑CGGCTGTTGCGGCACTAACAGCGTTTT‑ACCGCTCCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCtt                                                                    <  2:569029/239‑1 (MQ=255)                                                                                                                                                                           tAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTGCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCTTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCtt                                                                    <  1:1039089/239‑1 (MQ=255)                                                                                                                                                                           tAAGTT‑GTGGTTCTAACGTGCGACCAACCGCCGAC‑CCGCGATGACGCGCCACCCCTTCCAGATCAACCCCG‑TT‑CGGCTGTTGCTGCACTAACAGCTTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCt                                                                     <  1:654429/238‑1 (MQ=255)                                                                                                                                                                           tAAGTT‑GTGGGTGTAACGTGCGACCAAAGGCCGAC‑CGGCGATGACGCGCCAACCCTTCCAGATCACCACCG‑TT‑CGTCTGTTGTGGCGCAAACAGCGTTTT‑ACCGCTGCGGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCt                                                                     <  1:588664/238‑1 (MQ=255)                                                                                                                                                                                t‑gtgGTTGTACCGGGCGACCAAACGCCGAC‑CCGCGCTGCCGCGCCACCCCTCCCAGATCAACACCT‑TT‑CGGCTGTTGCTGCACTAACCGGGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                <  2:357694/238‑1 (MQ=255)                                                                                                                                                                                t‑gtgGTTGTAACGGGCGACCAATCGCCGAA‑CCGCGATTACGCGCCAACCCGTCCAGATCAACCCCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑CCCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                <  1:608167/238‑1 (MQ=255)                                                                                                                                                                                 tggggTTGTAACGCGAGCCAAAGCGCCGAA‑CCGCGTTGACGCGCCGACCCTTCCAGACCACCGCCG‑TT‑CGGCTGTTGCAGCACTAACAGCGTTTT‑ACCGCGGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCTCCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGca                                                                <  2:875172/235‑1 (MQ=255)                                                                                                                                                                                    ggtcgtgacgtacagctaaaCGTCGAC‑CCGCGATAACGCTCCAACCCCTCGAGAT‑AACCCCGTTT‑CGCCTGTTTCATCACAAACGGCGTTTT‑CCCGCTGACGGGATATCCGCCAATCAGCTCTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCTGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACaa                                                             <  2:837390/221‑1 (MQ=255)                                                                                                                                                                                      ttGTAACGAGCGAGCAAACGCCGAC‑CCGCGATGCCGCGCCAACCCTTCCAGACCAACCCCG‑TT‑CGGCTGTTGCTGCACCAACAGCGTTTT‑GCCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAata                                                           <  1:268827/238‑1 (MQ=255)                                                                                                                                                                                                cGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTg                                                >  1:576072/1‑239 (MQ=255)                                                                                                                                                                                                cGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAACGTTTT‑ACCGCTGCCGGTACAACCGCCAGTAAGCACGATCGGTTTTTGTGCCAGTTCAATAATCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCGTTCCACAATCGGAAAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAacgcgg                                                >  2:629057/1‑237 (MQ=255)                                                                                                                                                                                                       aCGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCCGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCACCTTTGCACAACCTGTGAGCGCTGACCGcc                                          >  2:707137/1‑238 (MQ=255)                                                                                                                                                                                                       aCGCCGAA‑CCGCGATGACGAGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTAGCTGCACTAAAAGCGTTTT‑ACCGCTGCCGGTACAAACGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCTGTCTCGCGCAGTGCCTTAAAACCGCCTTCCACCAGCGGATCATCAATCCCAGATGCATCCAACCAGCTTTGCACAATATGTGAGACCTGACCGca                                          >  2:766335/1‑237 (MQ=255)                                                                                                                                                                                                             aa‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGgc                                    >  2:416540/1‑238 (MQ=255)                                                                                                                                                                                                              cacccgcgctgcgccgaccccccTCCAGGACAACGCCC‑TTCCGGCGGTTCCTCCACTAACA‑CGTTTTCACCGCGGCCGGCACAACCGCCAATCAGCGCTATCGGTTTTTGTTCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCATAATATCCGCCTTCCACCAGCGGTTAATCAATCCCCGCAGCAGGCAACCAGCTTTGCACAATATGTGAGAGCTGACCGCCACGGgc                                    <  2:136908/220‑1 (MQ=255)                                                                                                                                                                                                                ccGCGATGCGCCACCACCCCACCCAGATCACCCCCT‑TT‑CGCTTGTGGCGCCACAAACATGTTTTT‑CCCCCGGCGGTTAAACCCGCCAATCAGCACTACCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCTTTCCACCAGCGGATAATCAATCCCCGCTGCAGGCAACCAGCTTTGCACAATATGTGAGCGCGGACCGCCACGGGCgca                                 <  2:131271/239‑1 (MQ=255)                                                                                                                                                                                                                ccGCGATGACGCGCCAACCCTCCCAAACCAACCCCG‑GT‑GGGCTGTGGTTGCACTAACAGCGTTTT‑CCCGCGGCCGGTACACCCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCgca                                 <  1:248313/239‑1 (MQ=255)                                                                                                                                                                                                                ccGCGATGACGCGCAAACCCTCCCAGATCAACCCCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCgca                                 <  1:376717/239‑1 (MQ=255)                                                                                                                                                                                                                 ggcgATGCCGCGCAACCCCTTCCAGATCACCGCCG‑TC‑CGGCTGTTGCTGCACTAACGGCTTTTT‑CCCGCTGCCGGTACACCCGCCAAGCAGCACTACCGGTTTTTGGGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCgcag                                <  2:576072/238‑1 (MQ=255)                                                                                                                                                                                                                 cgcaATGGCGCCCCACCCCTGCCAGAGCAACTCCC‑TC‑CGGCTTTTGCCTCTCTAACCGCTTTTT‑CCCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTGGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCTTTCCACCAGCGGATAATCAATCCCCGCGGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCgcag                                <  2:726346/239‑1 (MQ=255)                                                                                                                                                                                                                                aCCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTGGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACTTTgg                 >  1:1004217/1‑238 (MQ=255)                                                                                                                                                                                                                                          aTCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATcg                                                                                                                                           <  1:932934/107‑1 (MQ=255)                                                                                                                                                                                                                                          aTCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATcg                                                                                                                                           >  2:932934/1‑107 (MQ=255)                                                                                                                                                                                                                                               cacCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGCCCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCAc  >  1:155181/1‑239 (MQ=255)                                                                                                                                                                                                                                               cacCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCAc  >  2:150809/1‑239 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ATCTTCTACCACCGCAATCGCCGCCTGAGTCATTCGCTCGCGCAGGCATTCCGGCAGGTGATTCGAACCGATCATCCGGCTTTCGTCTTCCAGCACCCACAGGCGCAAATTCTGACGGGCGTCGGTTTTTAGCATTTCGGCAGCCAGCAGGTTTTCAAAACTCGCCTGGCTAAGTT‑GTGGTTGTAACGTGCGACCAAACGCCGAA‑CCGCGATGACGCGCCAACCCTTCCAGATCAACACCG‑TT‑CGGCTGTTGCTGCACTAACAGCGTTTT‑ACCGCTGCCGGTACAACCGCCAATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGTGAGCGCTGACCGCCACGGGCGCAGCAGAGAATACCTTGCGGATTTTGCAGGCAC  >  NC_000913/530495‑530969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑