Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,043,021	Δ1 bp	8.3%	intergenic (+123/+447)	yodB → / ← serU	cytochrome b561 homolog/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,043,020	0	A	.	8.3%	178.7 / 7.6	48	intergenic (+122/+448)	yodB/serU	cytochrome b561 homolog/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (19/25);  new base . (2/2);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-02

GAAACCTGGGCAAATATAGGCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTCCCACCACCAACTTTGCGGCAGTTTCATAGGATGA  >  NC_000913/2042782‑2043254                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           gAAACCTGGGCAAATATAGGCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACa                                                                                                                                                                                                                                            <  2:1185582/239‑1 (MQ=255)      cTGGGCAAATATAGGCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCATTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGt                                                                                                                                                                                                                                       <  2:216245/239‑1 (MQ=255)             aaTATAGGCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACtt                                                                                                                                                                                                                                <  1:589271/239‑1 (MQ=255)             aaTATAGGCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACtt                                                                                                                                                                                                                                <  1:93337/239‑1 (MQ=255)             aaTATAGGCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACtt                                                                                                                                                                                                                                <  2:1085052/239‑1 (MQ=255)                    gCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCaaaa                                                                                                                                                                                                                         >  1:403480/1‑239 (MQ=255)                       aCTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATa                                                                                                                                                                                                                      <  1:762269/239‑1 (MQ=255)                       aCTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATa                                                                                                                                                                                                                      <  1:1171290/239‑1 (MQ=255)                       aCTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATa                                                                                                                                                                                                                      <  1:13577/239‑1 (MQ=255)                       aCTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCAACACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATa                                                                                                                                                                                                                      <  2:435206/239‑1 (MQ=255)                                             tGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTaa                                                                                                                                                                                                 <  1:478423/238‑1 (MQ=255)                                                           ttCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGt                                                                                                                                                                                  <  1:755795/239‑1 (MQ=255)                                                                caCTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTa                                                                                                                                                                             <  1:1040890/239‑1 (MQ=255)                                                                caCTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTa                                                                                                                                                                             <  2:1178870/239‑1 (MQ=255)                                                                  cTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTAt                                                                                                                                                                            <  1:1028859/238‑1 (MQ=255)                                                                  cTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTAt                                                                                                                                                                            >  2:1028859/1‑238 (MQ=255)                                                                                         tGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTg                                                                                                                                                                                                         <  2:1203757/186‑1 (MQ=255)                                                                                         tGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTg                                                                                                                                                                                                         >  1:1203757/1‑186 (MQ=255)                                                                                         tGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACaa                                                                                                                                                                     <  2:1182352/222‑1 (MQ=255)                                                                                         tGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACaa                                                                                                                                                                     >  1:1182352/1‑222 (MQ=255)                                                                                         tGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCaaaaaa                                                                                                                                                       >  1:833239/1‑236 (MQ=255)                                                                                         tGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCaaaaaa                                                                                                                                                       >  2:882414/1‑236 (MQ=255)                                                                                          gTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCt                                                                                                                                                    >  2:840434/1‑238 (MQ=255)                                                                                          gTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCt                                                                                                                                                    >  1:198083/1‑238 (MQ=255)                                                                                              cGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAACTACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGaa                                                                                                                                               >  1:531129/1‑239 (MQ=255)                                                                                               gAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGaaa                                                                                                                                              <  1:840434/239‑1 (MQ=255)                                                                                                             cgcAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTG‑TAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTgg                                                                                                                               <  1:941150/239‑1 (MQ=255)                                                                                                              gcAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTgg                                                                                                                               <  1:377310/239‑1 (MQ=255)                                                                                                                          aTCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATa                                                                                                                                                                                                                      >  2:22923/1‑140 (MQ=255)                                                                                                                          aTCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATa                                                                                                                                                                                                                      <  1:22923/140‑1 (MQ=255)                                                                                                                                  aCAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCcg                                                                                                           <  2:403480/239‑1 (MQ=255)                                                                                                                                         aaTGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTAC‑ATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTa                                                                                                                                                                                       >  2:480726/1‑155 (MQ=255)                                                                                                                                         aaTGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTAC‑ATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTa                                                                                                                                                                                       <  1:480726/155‑1 (MQ=255)                                                                                                                                         aaTGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACACTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTAC‑ATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTa                                                                                                                                                                                       <  1:1023489/155‑1 (MQ=255)                                                                                                                                         aaTGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACACTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTAC‑ATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTa                                                                                                                                                                                       >  2:1023489/1‑155 (MQ=255)                                                                                                                                             tgAACCATGGACATCAACGCGTCATATATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTTGAAATTATGGACTTTGTCCCGCGGCTTTaa                                                                                                  >  1:754753/1‑237 (MQ=255)                                                                                                                                                                cgTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTa                                                                                                                                                                             >  1:369964/1‑143 (MQ=255)                                                                                                                                                                cgTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTa                                                                                                                                                                             <  2:369964/143‑1 (MQ=255)                                                                                                                                                                             tACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAattat                                                                >  2:139751/1‑239 (MQ=255)                                                                                                                                                                                            tGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAATAAAGCTGCGAAATTCAACAACTCTTGGAATTTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTAc                                                 >  1:859453/1‑239 (MQ=255)                                                                                                                                                                                                 ccTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCa                                              >  1:828525/1‑237 (MQ=255)                                                                                                                                                                                                         cTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTc                                    <  1:882414/239‑1 (MQ=255)                                                                                                                                                                                                           aTCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTcc                                   >  2:1122737/1‑238 (MQ=255)                                                                                                                                                                                                                          cAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTCCCACCACCAACTTTGCg                   >  1:1036073/1‑239 (MQ=255)                                                                                                                                                                                                                               aCTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCtt                                                                                                                                 >  2:1110078/1‑124 (MQ=255)                                                                                                                                                                                                                               aCTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCtt                                                                                                                                 <  1:1110078/124‑1 (MQ=255)                                                                                                                                                                                                                                        aTCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTCCCACCACCAACTTTGCGGCAGTTTCATAGGa     >  2:296240/1‑239 (MQ=255)                                                                                                                                                                                                                                           gTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTCCCACCACCAACTTTGCGGCAGTTTCATAGGATGa  <  1:139751/239‑1 (MQ=255)                                                                                                                                                                                                                                           gTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTCCCACCACCAACTTTGCGGCAGTTTCATAGGATGa  <  2:833239/239‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           GAAACCTGGGCAAATATAGGCTACTTTTTAATCGCAGCTCACGCTGGCGCAGCACTCTTTCATCACTACATTCAGAAAGATAATACTCTGTTACGAATGATGCCTCGCCGCAAATAAGTCGATCCCCTTACAAGGAAATGTGAACCATGGACATCAACGCGTCATAAATGTTTACGCAAGTGCACAATGCACCCTTTCCACTATCAACAAGGAGGAGCAGAAACTGTGTAAATCGTACAATGGTAAGACTTATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGACGGCCGCGTTCCTTTTTTGACTCAATTATTGAACCCTTCTCTACGCAACTTCAGTTCCCACCACCAACTTTGCGGCAGTTTCATAGGATGA  >  NC_000913/2042782‑2043254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑