Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 599,612 T→G 14.2% V300G (GTG→GGG)  cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,6120TG14.2% 253.6 / 5.7 107V300G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (19/65);  new base G (14/4);  total (38/69)
Fisher's exact test for biased strand distribution p-value = 1.69e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTAT  >  NC_000913/599382‑599840
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      
aCTGGCGGAAGGGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATcgc‑cg                                                                                                                                                                                                                               <  2:1038693/239‑1 (MQ=255)
aCTGGCGGAAGCTGAATGTCTGGTGCGCGCCAGCGGCTATCTGAAAACGCTCGACGACTTTAATCACACCGTTTTAAAAGCCAGTGAAAATGGCGGGCCCGTTTACCTGCGCGATGTTGCAAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGTGGTGATCCT‑GCTATCCGGAAAAAACGCCCGAGAAGTGATcgc‑cg                                                                                                                                                                                                                               <  2:990197/239‑1 (MQ=255)
      ggaagcggaaTATATGGTGCGCGCCAGCGGGTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTg                                                                                                                                                                                                                          <  1:844022/238‑1 (MQ=255)
                     ggTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGaaa                                                                                                                                                                                                          <  1:909146/239‑1 (MQ=255)
                     ggTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGaaa                                                                                                                                                                                                          <  1:697822/239‑1 (MQ=255)
                      gTGCGCGCCAGGCGTTATCGGCAAACGCTCGACGACTTTAATCCCATCGGTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGCCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGaaa                                                                                                                                                                                                          <  2:290898/238‑1 (MQ=255)
                       tGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCT‑GCGATCCGGCAAAAAAGCCCGAGGAGAGATCGC‑CGCCGTGAAGGGCAAACTGGAAAc                                                                                                                                                                                                         >  1:244688/1‑238 (MQ=255)
                       tGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCT‑GCGGTCCCGCAAAAAAGCCCGAGAAGTGATCGC‑CGCCGGGCAGGACAAACTGGaaaag                                                                                                                                                                                                        >  1:1024399/1‑237 (MQ=255)
                        gcgcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTGCT‑GCGGGTCCACAACAAAGCCCCGGAAGCGAGCGG‑CGCCGGGAGGGCCAACATGGaaaag                                                                                                                                                                                                        >  2:684545/1‑236 (MQ=255)
                        gcgcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCT‑GCGAACCGGCCAAAACGCCCCGGGAGTGGTCGC‑CGCCGGGGAGGGACAACTGGtaaacc                                                                                                                                                                                                       >  1:26968/1‑236 (MQ=255)
                        gcgcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACg                                                                                                                                                                                                        <  2:396106/238‑1 (MQ=255)
                        gcgcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATTCT‑GCGGACCCGCCAAAAAGCCCGGGAAGAGGTCGC‑CGGCGGGCAAGGCAAACCGGgaaag                                                                                                                                                                                                        >  1:420298/1‑236 (MQ=255)
                        gcgcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGAGCCT‑GCGATCCGGCAAAAACGCCCGGGAAGGGAGCGC‑CGCCGTGAAGGACAAACTTGGAACg                                                                                                                                                                                                        >  1:607576/1‑238 (MQ=255)
                         cgcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCT‑GCGGTCCGGCAAAAACGCCCCGGGAGGGATGGC‑CCCCGGGAAGGGCACAATGGGAAAGcc                                                                                                                                                                                                      >  1:264448/1‑238 (MQ=255)
                          gcgcCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGCCC‑CCGGTCCGGCAAAAAAGGCCGGGAAGGGAGTGC‑CCGCGTGGAGGGGAAACTGGAAAAGCta                                                                                                                                                                                                     >  2:909146/1‑238 (MQ=255)
                                gCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCT‑GCGCGCCGGCCAAAACGCCCGGGGAGGGATAGC‑CGCCGCGGAGGACAAAATGGAAAAGCTGGAAaga                                                                                                                                                                                               >  1:1109940/1‑238 (MQ=255)
                                 cGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATTCTGGCGTCGAGGCAAAGAAGCCCG‑GAAG‑GAACGCGCGCCGGGGAGGGCAAAATGGGAACGATGGAAagga                                                                                                                                                                                              >  2:174893/1‑237 (MQ=255)
                                    cTATCTGCAAACGCTCGACGATTTTAATCACAGCGTTTTAAAAGCCAGTGAAAAGGGCGTGCCCGTTTATCTGCGCGATTTTGCGAAGGCCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAagtagt                                                                                                                                                                                            <  2:469732/238‑1 (MQ=255)
                                      aTCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCT‑GCGATCCGGCAAAAACGCCCCGGAAGGGATCGC‑CGCCGTGAGGGGCAAACTGGAAAAGCTGAAAAGCAGTCt                                                                                                                                                                                          >  1:34500/1‑238 (MQ=255)
                                           aaaaCGCTCAACGACTTGAATCACATAGTTTTAAAAGCCAGGGAAAAGGGCGGGCCCTCTTAGCTGCGCGAGGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATCGCCGACCTAAACGGCGAAGGCGAAGGGGCGGGCGGGGTGGTGATCCA‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa                                                                                                                                                                                    <  2:28894/238‑1 (MQ=255)
                                            aaaCGCTCGACGACTTTAAGCACATCGTTTTAAAGGCAAGGGAAAATGGCGTGCCCGTTTATCTGCGCGAGGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAGCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa                                                                                                                                                                                    <  1:422985/238‑1 (MQ=255)
                                               cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                                                                                                                                                <  1:676049/239‑1 (MQ=255)
                                                gCTCGACGGCTTTAATCTCATCGTTTTAAAAGCCAGTGAAAGTGGCGGGCCCGTGTGCCTGCGCGAGGTTGCGAAGGTCCAGATTGGCGCGTGGTTGCGTGGGTGCTTTTCCTGACTAAAGGGGGAGGGCGTCGTGGCGTGGGGGGTGGT‑TTCCTGGTCATCCGCCACAACGGCCCGTGAGTTGCCCGC‑CGCCGTGAAGGCCAAACTGCAAACGCTGAAAAGTAGTCTGCCGGAAGGCg                                                                                                                                                                               <  2:430119/239‑1 (MQ=255)
                                                  tCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTg                                                                                                                                                                             <  2:665087/239‑1 (MQ=255)
                                                    gacgacTTTAAGCACAACGTTTTAAAAGCCAGGGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGga                                                                                                                                                                           <  2:1252032/239‑1 (MQ=255)
                                                      cggcTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                                                                                                                                          <  1:802671/235‑1 (MQ=255)
                                                      cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                                                                                                                                          <  2:399970/238‑1 (MQ=255)
                                                      cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                                                                                                                                          <  1:124880/238‑1 (MQ=255)
                                                       gtcTTTAATCACATGTTTTTAAAAGCCAGTGAAAATGGCGTGCCCGGTGATCTGCGCGATGTAGCGAAGGTGCAGAATGGACTGGAGAAGCGGCGGGGCATTGCCGAACTAAACGGCTAAGGCGAAGTCGCGGGCGGAGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑AGCCGTGAAGGACAAACTGGAAACGATGAAAAGTAGTCTGCCGGAAGGCGTGGAGAt                                                                                                                                                                        <  2:471993/237‑1 (MQ=255)
                                                        acTTTAATCACCTCGTTTTAAAAGCCAGGGAAAATGGCGTGCCCGTTTAGCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGAt                                                                                                                                                                        <  1:173874/238‑1 (MQ=255)
                                                         cTTTAATCACATCGTTTTAAAGGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAg                                                                                                                                                                      <  1:956847/239‑1 (MQ=255)
                                                          tttAATCCCATCGTTTTAAATGCCAGTGAAAATGGCTTGCCCGGTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                                                                                                                                     <  2:624783/239‑1 (MQ=255)
                                                          tttAATCACATCGTGTTAAAAGCGAGTGAAAAGGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                                                                                                                                     <  2:1241950/239‑1 (MQ=255)
                                                          tgtAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCAATCCGGCAAAAACGCCCGGGAAGTTATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                                                                                                                                     <  2:169453/237‑1 (MQ=255)
                                                            tAATCATATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTGTATCTGGGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGAATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTa                                                                                                                                                                   <  2:1045404/239‑1 (MQ=255)
                                                            tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTa                                                                                                                                                                   <  1:520562/239‑1 (MQ=255)
                                                            tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTACCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTa                                                                                                                                                                   <  2:1046450/239‑1 (MQ=255)
                                                            tAATCACATCGTTTAAAAAGTCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTa                                                                                                                                                                   <  1:1224601/239‑1 (MQ=255)
                                                             aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTac                                                                                                                                                                  <  2:799325/239‑1 (MQ=255)
                                                                   aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGGTCCC‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGGGAAGGACAAACTGGAAAACCGGAAAAGGAGTCTGCCGGGAGGCGTGGGGGTAGTTAAAAAATa                                                                                                                                                            >  1:694675/1‑239 (MQ=255)
                                                                              aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcc                                                                                                                                                 <  1:116483/239‑1 (MQ=255)
                                                                              aGCCAGTGAAAATGGCGTGCCCGTTGATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGAGGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GAGATCCGGCAAAAACTCCCGAGAAGTGATCGC‑CGCAGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcc                                                                                                                                                 <  1:857588/239‑1 (MQ=255)
                                                                                      aaaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAagtagt                                                                                                                                                                                            <  1:355223/188‑1 (MQ=255)
                                                                                              gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCC‑CCCGCCCCGCCAAAACGCCCCCGAAGGGATTGC‑CGCCGGGAAGGCAAAACTGGGACACCTGAAAAGAAGGCTGCCGGGAAGCGGGGAGATAGTGTCAACACACGGACCAAGCCCACCCAATG‑ACcgcg                                                                                                                                 >  2:1141110/1‑238 (MQ=255)
                                                                                              gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCT‑GCGATCCGGCCAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGGCAAACCGGAAACGCTGAAGAGGAGTCTGCGGGAAGGCGCGGAGGCAGTTGAAACATACGAACGCCGCCAGCTCATTG‑ccccgg                                                                                                                                 >  2:185612/1‑235 (MQ=255)
                                                                                              gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTGCC‑GCGGTCACGCAAAAAAACCCGGGAAGTGGACGG‑CGCCGAGAAGGACAAACGTGAGAAGATGACAAGAAGGGTGCCGGAAGGCGCGGGGGGCGTGACAACATAAGACGGCAGACAGATCATTGTACcgc                                                                                                                                  >  2:798406/1‑238 (MQ=255)
                                                                                                          cTGCGCGATGTTGCGAAGGTCCAGTTTGGCCCGGAGATGGGCCGGGGAATTGTCGAACAAATCGGGGAAGGCGAGGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑GCCGCGCCATCGACAACCt                                                                                                                    <  2:327583/239‑1 (MQ=255)
                                                                                                          cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCt                                                                                                                    <  1:161797/239‑1 (MQ=255)
                                                                                                              gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCT‑GCCATCCGGCAAAAACGCCCGAGAAGGGATCGC‑CCCCGCGAAGGACAAACTGGAAACGCTGCAAAGAAGTCTGTCGGAAGGCGTGGAGAGAGATACAACATACGATCGCAGCCAGCTCCTTG‑ACCGCGCCATAGACAACCTCaac                                                                                                                >  2:468174/1‑237 (MQ=255)
                                                                                                                     ggCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGt                                                                                                         <  1:711316/238‑1 (MQ=255)
                                                                                                                            gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCT‑GCGATCCGGCAAAAAAGCCCGAGAAGGGAGCGC‑CGCCGGGGAGGGCAAACTGGGAAAGCTGAAAAGTAGTCTGCCGGGAGGGGGGGGGGTAGGTTCAACATACGGACGCAGCCAGCTCATTT‑ACCGCGCCCTCGCCAACCTCAGCGGCCAGTTGCTGGa                                                                                                  >  2:223064/1‑239 (MQ=255)
                                                                                                                            gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCT‑GCGGTCCGGCAAAAACGCCCGAGAAGAGATCGC‑CGCCGGGAAGGACAAACTGGAAAAGCTGAAAAGGAGTCGGCCCGCAGGCGTGGGGATAGTTTCAAAAAACGATCGCAGCCCGCTCCTTT‑ACCGCGCCCTCGACAACCTCCGCGGCACGCTGCTggg                                                                                                  >  2:1049530/1‑238 (MQ=255)
                                                                                                                            gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCT‑GCGATCCGGCAAAAACGCCCGGGGAGTGATCGC‑CGCCGGGAAGGGCAAACCGGAAAAGCTGGAAAGTAGTCTGCCGGAAGGCGGGGGGATAGGTACAAAATACGAGCGCAGCCAGCTCATTG‑ACCGCGCCATCGGCAACCTCAGAGGCCAGTTGCTGGa                                                                                                  >  2:386360/1‑239 (MQ=255)
                                                                                                                             tCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGa                                                                                                  <  2:155994/238‑1 (MQ=255)
                                                                                                                             tCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGGGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGa                                                                                                  <  2:63327/238‑1 (MQ=255)
                                                                                                                               gagagTGGCCGGGAGATGCGCCGGGGCATTGCAGAACTAAACGGCGAAGGCGAAGTGGCGGGAGGGGTGGTGTTCCT‑GCGATCCGGCAAAAACGCCCGAGAAGGGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAaga                                                                                               <  2:1171183/238‑1 (MQ=255)
                                                                                                                               cAGATGGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAaga                                                                                               <  1:405590/239‑1 (MQ=255)
                                                                                                                                      gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                                                                                                                                     >  1:998343/1‑163 (MQ=255)
                                                                                                                                      gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGGGATCGC‑CGCCGTGAAGGGCAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGGGGTAGTTACAACATACGATCGCAGCCAGCTCCTTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGGAGAGGTTAtt                                                                                        >  1:27503/1‑239 (MQ=255)
                                                                                                                                      gCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCT‑GCGATCCGGCAAAAACCCCCGAGAAGGGATCGC‑CGCCGGGAAGGAAAAACTGGAAAAGCTGAAAAGAAGGCTGCCGGAAGGCGTGGGGGTAGGTAAAACATAAGATCGCGCCCCGCTCATTT‑GCCCCGCCCCCGCAAACCCCCACGGGAAGTTTGTGGGAGAGGTtttt                                                                                        >  2:981924/1‑239 (MQ=255)
                                                                                                                                        cgggAGATGGGCCGGGGAATTGCCGAACTAAACGGGGAAGGCGAAGTGGCGGGCGGGGTGGTGAACCG‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATtgt                                                                                      <  2:607576/237‑1 (MQ=255)
                                                                                                                                          ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTgtgg                                                                                    <  1:115755/239‑1 (MQ=255)
                                                                                                                                                gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCT‑GCGATCCGGCAAAAACGCCCGGGGAGTGAACGC‑CGCCGGGAAGGACAAACTGGAAAAGCTGAAAAGGAGTCTGCCGGAAGGCGGGGGGATAGTTACAAAAAACGATCGCAGCCAGCTCATTT‑ACCGCGCCCTCGACAACCTCAGCGGCAAGGTGCTGGGAGGGGTTATTGTGGTTGCgg                                                                              >  2:1105960/1‑239 (MQ=255)
                                                                                                                                                 cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcc                                                                                                                                                 <  1:1231718/172‑1 (MQ=255)
                                                                                                                                                 cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCT‑GCGATCCGGCAAAAACGCCCGCGAAGGGATCGC‑CGCCGTGAAGGACAAACTGGGAAAGCTGGAAAGTAAGCTGCCGGAAGGCGGGGGGATAGTTACAACAAACGAACGCAGCCAGCCCATTG‑ACCGCGCCATCCACAACCCCAACGGCCAGATGTTGGGAGAGTTTTTTGTggggggggg                                                                             >  2:1192365/1‑238 (MQ=255)
                                                                                                                                                 cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATTCT‑GCTAGCCGGCAAAAACGCCCGAGAAGTGATCGC‑CCCCGTGGAGGACAAACCGGTAACGCTGCAAAGAAGTCTGCCGGACGGCGGGGAGATATTTACAACATACGATCGCAGCCCGCTCCTTG‑ACCGCGCCATCGGCAACCTCAGAGGCAAGTTTCTGGCGAAATTTATTGttgtgggcgg                                                                             >  2:270348/1‑235 (MQ=255)
                                                                                                                                                      gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAg                                                                           >  1:713397/1‑236 (MQ=255)
                                                                                                                                                          aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCACCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTgc                                                                    <  1:716350/239‑1 (MQ=255)
                                                                                                                                                           ttGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCT‑GCGATCCGGCAAAAAAGCCCGAGAAGGGATCGC‑CGCCGTGAAGGACAAACTGGGAACGCTGCAAAGAAGTCTGCCGGGAGGCGGGGGGGTAGTTACAACATACGAACGCAGCCCGCCCATTG‑a                                                                                                                                      >  2:1231718/1‑172 (MQ=255)
                                                                                                                                                            tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTgcgc                                                                  <  1:223064/239‑1 (MQ=255)
                                                                                                                                                            tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGGGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGGCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTgcgc                                                                  <  1:48660/239‑1 (MQ=255)
                                                                                                                                                               caaaCTACACGGGGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCG‑GCGATCCGGCAAAAACGCCCGAGAAGGGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGTCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTg                                                                <  2:676/236‑1 (MQ=255)
                                                                                                                                                               cGAACTAAACGGCGAAGGTGAAGGGGCGGGGGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTg                                                                <  2:611233/238‑1 (MQ=255)
                                                                                                                                                                gAACAAAACGGGGAAGGCGAAGTGGCGGGCGGGGTGGTGAACCT‑GGGAGACGGCAAAAACGCCCGAGAAGTGAGCGC‑CGACGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTAGTGTGGTGGCGGTAGTCTGTGCGCTGtt                                                              <  2:1204134/239‑1 (MQ=255)
                                                                                                                                                                      aaCGGCGAAGGTGAATGGGCGGGCGGGTTGGTGATCCT‑GCGGACCGGCAAAAACGCCCGAGAAGGTATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATTCGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTg                                                        <  2:1092799/239‑1 (MQ=255)
                                                                                                                                                                                          gggcgggGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCtg                                     <  1:909692/238‑1 (MQ=255)
                                                                                                                                                                                                          cT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGtt                    >  1:305996/1‑239 (MQ=255)
                                                                                                                                                                                                          cT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGtt                    <  2:420298/239‑1 (MQ=255)
                                                                                                                                                                                                          cT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGtt                    >  1:364302/1‑239 (MQ=255)
                                                                                                                                                                                                          cT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGtt                    <  1:674289/239‑1 (MQ=255)
                                                                                                                                                                                                          cT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGtt                    >  2:898800/1‑239 (MQ=255)
                                                                                                                                                                                                          cT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGGGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGtt                    >  1:1121845/1‑239 (MQ=255)
                                                                                                                                                                                                             gcgcTCCGTCAACAACGCGCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGATGAAAAGAAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCACGTTGCTGGAAGAGATACTTGTGGTGGCGGGAGTCTGTGCGTTGTTTCTCTGGGATGTGCGCTCGGAGCGGGTGGGGGTCATTTCGttc                   >  2:726243/1‑237 (MQ=255)
                                                                                                                                                                                                              cgacacGGCAAAAACGCCCGAGAAGTGATGGC‑CGCCGTGAAGGACAAACTGGAAACGGTGAAAAGGAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGc                  <  2:34500/233‑1 (MQ=255)
                                                                                                                                                                                                               gATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAAAATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGATTATTGTGGTGGCGGTAGCCTGTGCGCTGTTTCTCTCGAATATGCTCTCGGCGCCGGTGGCGGTTATTTCGTTGcc                 >  2:779273/1‑238 (MQ=255)
                                                                                                                                                                                                                aTCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCg                >  1:199940/1‑238 (MQ=255)
                                                                                                                                                                                                                 tCCGGCAAAAACGCCGGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGc               <  2:27503/238‑1 (MQ=255)
                                                                                                                                                                                                                 tCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGc               <  2:364302/238‑1 (MQ=255)
                                                                                                                                                                                                                 tCCGGCAAAAACGCCAGAGAAGTGATGGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGc               <  2:244688/238‑1 (MQ=255)
                                                                                                                                                                                                                 gccGGCAAAAACGCCCGAGAAGTGATCGG‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGGGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGc               <  1:886348/237‑1 (MQ=255)
                                                                                                                                                                                                                  ccGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTg             <  2:233225/239‑1 (MQ=255)
                                                                                                                                                                                                                  ccGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTg             <  1:1104793/239‑1 (MQ=255)
                                                                                                                                                                                                                  ccGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTg             <  2:713397/239‑1 (MQ=255)
                                                                                                                                                                                                                     gCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGa                                                                                                                          >  2:633317/1‑127 (MQ=255)
                                                                                                                                                                                                                     gCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGa                                                                                                                          <  1:633317/127‑1 (MQ=255)
                                                                                                                                                                                                                      cAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTgggg          <  1:1035238/238‑1 (MQ=255)
                                                                                                                                                                                                                       aaaaaCGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGtt        <  2:264448/239‑1 (MQ=255)
                                                                                                                                                                                                                             gCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTAt  <  1:185612/239‑1 (MQ=255)
                                                                                                                                                                                                                             gCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTAt  <  1:999239/239‑1 (MQ=255)
                                                                                                                                                                                                                             gCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTAt  <  2:234155/239‑1 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      
ACTGGCGGAAGCGGAATATATGGTGCGCGCCAGCGGCTATCTGCAAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCT‑GCGATCCGGCAAAAACGCCCGAGAAGTGATCGC‑CGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTG‑ACCGCGCCATCGACAACCTCAGCGGCAAGTTGCTGGAAGAGTTTATTGTGGTGGCGGTAGTCTGTGCGCTGTTTCTCTGGCATGTGCGCTCGGCGCTGGTGGCGATTATTTCGTTGCCGCTGGGGTTGTGTAT  >  NC_000913/599382‑599840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑