Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	836,625	T→G	14.7%	V92G (GTC→GGC)	ybiC →	putative dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	836,625	0	T	G	14.7%	162.7 / 3.4	70	V92G (GTC→GGC)	ybiC	putative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (11/45);  new base G (12/2);  total (23/47)
Fisher's exact test for biased strand distribution p-value = 7.85e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCT  >  NC_000913/836393‑836860                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             tttATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTcgc‑g                                                                                                                                                                                                                                         <  2:142233/237‑1 (MQ=255)    agtCAGTCTGTATTTCGTCAGATGGGTAGCGAGTAACAAGAAGCTAAATTAGTTGCCGATCATTTAATCGCGGCAAGCCAGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCGCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGcaca                                                                                                                                                                                                                                    <  2:49950/237‑1 (MQ=255)    aTTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGcaca                                                                                                                                                                                                                                    <  2:738949/239‑1 (MQ=255)    aTTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGcaca                                                                                                                                                                                                                                    <  2:505696/239‑1 (MQ=255)     ttCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGGCGC‑GGCACAt                                                                                                                                                                                                                                   >  1:1149796/1‑239 (MQ=255)      tCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATg                                                                                                                                                                                                                                  <  2:1149796/239‑1 (MQ=255)          gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCCCCCCCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGc                                                                                                                                                                                                                              >  1:1199808/1‑239 (MQ=255)          gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCCC‑GGCACATGAAGc                                                                                                                                                                                                                              >  1:1038776/1‑239 (MQ=255)          gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCACTTGC‑GATCGCG‑CTTTTGGTACAGTCGC‑GGGACATGaatc                                                                                                                                                                                                                              >  1:1058052/1‑237 (MQ=255)             gTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGAt                                                                                                                                                                                                                           <  2:463047/239‑1 (MQ=255)              tATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCCCATGAAGCGATg                                                                                                                                                                                                                          >  1:6982/1‑239 (MQ=255)               aTTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATg                                                                                                                                                                                                                          <  2:90531/238‑1 (MQ=255)                   cGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGTCACGCTCGCTGGC‑GAACCCG‑CCTTTTGGCAGGGCGC‑GGCACATGACGCGGTGgcgcc                                                                                                                                                                                                                     >  2:484789/1‑238 (MQ=255)                        gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGTCACGCTCGATTGG‑GATCTCG‑CATTTGGGCAGGTCGC‑GGCCCACGAAACGATGGGGCCGGGGa                                                                                                                                                                                                                >  1:1093936/1‑239 (MQ=255)                        gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGG‑GGGCCCG‑CCTTTGGTGAGGGGGC‑GGGCCATAAAGCGAGCGCGCTggggg                                                                                                                                                                                                                >  2:499975/1‑238 (MQ=255)                        gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTggggg                                                                                                                                                                                                                >  1:643172/1‑238 (MQ=255)                        gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCCCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCCCCCCCCTGGC‑GAGCGCG‑CACTTGTGCACGGCGG‑GGCCCCTGGAGCGGTGGGGCCggggg                                                                                                                                                                                                                >  2:74212/1‑238 (MQ=255)                             gTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCCCGCTCGGCGGCGGCACCCG‑C‑CTTGGGCCGGTCGCGGGGCCATGAGGCGCTGGCGCGGGGGATTgg                                                                                                                                                                                                            >  2:877542/1‑238 (MQ=255)                                  gAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAg                                                                                                                                                                                                       <  2:551010/238‑1 (MQ=255)                                   aGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCTCGATGGC‑GAACGCG‑CATTTTGGTAGGTCGC‑GGCACATGAAACGGTGGGGCTGGGGGTTggggaaag                                                                                                                                                                                                      >  1:850897/1‑231 (MQ=255)                                   aGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGC‑GATCGCG‑CCTTTTGGCAGGGCGC‑GGGACATGAAGCGAGGGGGCCGGGGGTTGAGaaaagg                                                                                                                                                                                                     >  1:699259/1‑236 (MQ=255)                                       actggagtcGAAAATAGTTGCCGATCTTTTAATCGCGGCAATCCTGTCAGCTCATGATTCACATGGTATTCGCATGATCCCAATCTATGTACAGTCCTCGAGTCAGGGGCACCTGGAAATCAACCTTCATGCGAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGGTCGATGGC‑GATCGCG‑CATTTGGTCAGGGCGC‑GGCATATGAAGCAATGGCGCTGGGGATTGAGAAAGCGCAt                                                                                                                                                                                                  <  2:1058052/230‑1 (MQ=255)                                             agCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc                                                                                                                                                                                            <  2:553288/238‑1 (MQ=255)                                             agCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg                                                                                                                                                                                           <  1:418498/239‑1 (MQ=255)                                                                       tCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAAc                                                                                                                                                                 <  1:606073/239‑1 (MQ=255)                                                                        cgcgGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACt                                                                                                                                                                <  2:1038776/239‑1 (MQ=255)                                                                                          gCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGcc                                                                                                                                                 <  1:856714/236‑1 (MQ=255)                                                                                                 tCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTa                                                                                                                                       <  2:305889/239‑1 (MQ=255)                                                                                                   acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                                                     <  1:415496/239‑1 (MQ=255)                                                                                                   acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                                                     <  1:499975/239‑1 (MQ=255)                                                                                                   acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGAAGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                                                     <  1:775059/239‑1 (MQ=255)                                                                                                         tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAAc                                                                                                                                                                 <  1:206197/205‑1 (MQ=255)                                                                                                         tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGc                                                                                                                                                             <  1:982297/209‑1 (MQ=255)                                                                                                         tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCg                                                                                                                                <  1:854607/238‑1 (MQ=255)                                                                                                         tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCg                                                                                                                                <  2:643172/238‑1 (MQ=255)                                                                                                           ttGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAg                                                                                                                             <  1:561357/239‑1 (MQ=255)                                                                                                                   aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCCCGATGGC‑GATCGCG‑CATTTGGGCCGGGGGC‑GGCACCTGAAGCGAGGGGGCCGGGGGTTTAGAAAGGGCCTCAGCACGGGATTTCCGCCGGGGCGCGACATAACTCCCACCACAACGGCCGTATCGGGTGCTTGGGGGGGCAGtgtgg                                                                                                                     >  2:58087/1‑238 (MQ=255)                                                                                                                   aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCAGCTCGATGGC‑GATCGCG‑CCTTTGGTCAGGGCGC‑GGGCCATGAAGAGGTGGCGCTGGGGATTGGGAAAGCGCATCAGCACGGTATTTGCGCCGTGGGGCTCCATTACACCCATCCTACAGGCCGTATCGGTTACTGGGGGGAGGAGggtgt                                                                                                                     >  2:764108/1‑238 (MQ=255)                                                                                                                           cTAGGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGGCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCGACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGa                                                                                                                              <  1:534212/222‑1 (MQ=255)                                                                                                                                     tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGTCACCCCCCATGGC‑GATCCCG‑CCTTTTGTCCGGTCGC‑GGCACCTGAAACCATGGGGGCGGGGGTTTAGGAAGGGCAACCACCCGGGGGTTGCGCCCGGGCGCCCCCTAACTCGCATCCTCTCGGCCGGATCGGGTTCTGGGGGGGGCCgtggggcgctgtcttttttacac                                                                                                    >  2:534212/1‑218 (MQ=255)                                                                                                                                     tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGGCACGCCCGATGGC‑GATCGCG‑CCTTTGGGCCGGGCGC‑GGGACATGAAGCGATGGCGCTGGGGGGTGAGAAAGCGCATCAGCACGGTATTGGCGCCGGGGCGCGACAATACTCGCATCCTATCGGGCGGGTCCGGTACTGGGGGGGGCCGGGGGCAGGGGCggggggtgtg                                                                                                     >  2:559497/1‑231 (MQ=255)                                                                                                                                     tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGtctc                                                                                                   <  1:722284/239‑1 (MQ=255)                                                                                                                                     tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCCGCCCCGAGGC‑GAACGCGCCTTTTGGTC‑GGGCGC‑GGGCCATGAAGCGGAGGGGCCGGGGGGTGGGAAAACGGCTCAGCACGGGGTTGGCGCCCGGGGGGTTCATAAACCGCCTCCTATCGGCCGGATCGGTTTCTGGGGGGGGCCGCGGGGAGCGggggggggtggg                                                                                                     >  2:669967/1‑231 (MQ=255)                                                                                                                                            gTCAGGTGCACCTGCAAATTAACCATCATGCCAAACCCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                             <  2:371621/238‑1 (MQ=255)                                                                                                                                            gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                             <  1:484789/238‑1 (MQ=255)                                                                                                                                            gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                             <  1:764108/238‑1 (MQ=255)                                                                                                                                               aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCGACGGC‑GAGCGCG‑CATTTTGCCAGGGCGC‑GGCACATGAAGCGAGGGCGCTGGGGATTGGGAAAGCGCCTCCGCACGGTAGTGACGCCGGGGCGCTGCCAAACTCCCATCATATCCGCCGCATCGGTTACTTGGGGGAGCCGGGGGGAGCGGCGGGGGTGGTCTCTATcacccat                                                                                         >  2:37490/1‑233 (MQ=255)                                                                                                                                               aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCAACCCCGATGGC‑GATCCCG‑CCTTTGGGCAGGGCCG‑GGCACATGAAACGATGGCGCGGGGGGGTGAGAAAACGCCGCAGCACCGGATTTCCGCCGGGGCGCTACATAAACCGCATCCAATCGGGCGGATAGGTTACGTGGCGGGGCAGAGTGGAGCGGCGGGGGTTTtctctctctcacct                                                                                         >  2:1115487/1‑233 (MQ=255)                                                                                                                                                 gggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGC‑CAACCCG‑CCTTTTGTCTGGGCCC‑GGCCCCTTAAACGGTGGGGCCGGGGGGTGAGAAAGGGCACTAGCCCCGGTTTGCCCCCGTGGCGCCACATAAATCGCAACCTCTTCGCCCGATCCGTTTCTGGGCGGGGGCGTGGGGCGCGGGGGGGGGTGtctttttttcctttct                                                                                       >  2:110097/1‑226 (MQ=255)                                                                                                                                                           aaaTTAACCATCATGCCAAAACCGTTAAAGGGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTGGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:81468/238‑1 (MQ=255)                                                                                                                                                           aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCTCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCTGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:100025/238‑1 (MQ=255)                                                                                                                                                           aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  1:767429/238‑1 (MQ=255)                                                                                                                                                           aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  1:747258/238‑1 (MQ=255)                                                                                                                                                           aaaTTAACCATCAGGCAAAAACGGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:396679/238‑1 (MQ=255)                                                                                                                                                           aaaTGAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:850897/238‑1 (MQ=255)                                                                                                                                                              ttAACCATCATGCGAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGt                                                                          <  2:851714/239‑1 (MQ=255)                                                                                                                                                                                   atAAAGAGGCGGGGGCGGCGGGCACGGTCGAGGGC‑GAGCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCACCAGTACGGTATTGCCGCCGTGGCGCTACATAACCCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGGGGCGGGGTTTGTCTCTATCCACTTTTTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTc                                                     <  2:545498/238‑1 (MQ=255)                                                                                                                                                                                     aaaGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCa                                                   <  1:662848/239‑1 (MQ=255)                                                                                                                                                                                                   ggcggTCCCGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCAGCAGCCCGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGc                                      <  2:699259/238‑1 (MQ=255)                                                                                                                                                                                                   ggcggTCACTCTGGAGGGC‑GATCGCG‑CATTTGGGCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGc                                      <  2:466746/238‑1 (MQ=255)                                                                                                                                                                                                         cACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCttt                                <  1:917839/238‑1 (MQ=255)                                                                                                                                                                                                            gCTGGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACGCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                            <  1:669967/239‑1 (MQ=255)                                                                                                                                                                                                            gCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAGGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                            <  1:877542/239‑1 (MQ=255)                                                                                                                                                                                                            gCTAGAGGGC‑GATAGCG‑CATTTGGTCGGGTCGC‑GGCACATGAAGCGATGGGGCGGGGGAGTGAGAAAGCGCATCAGCACGGTGTTGCCGCCGTGGCGGTACATAACTAGGATCATATCGGCCGTATCGGTTTCGGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTAACCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGGTCCACGGTCGCGACAGCCGCTTTGGCa                            <  2:762298/239‑1 (MQ=255)                                                                                                                                                                                                              tCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCAcc                          <  1:1088418/239‑1 (MQ=255)                                                                                                                                                                                                                  tGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCCCATGAAGCGATGGCGCTGGGGATGGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAAt                       <  2:186340/238‑1 (MQ=255)                                                                                                                                                                                                                                 tttGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTTTCTATCCACTTTGTTATCGTGGGCGGGATTCCCATGGTCGCCCCGTTTCACGGTCGCGGCAGCCGCTTTGGCACCCATCCGGTCCGTGTggg         >  2:217365/1‑238 (MQ=255)                                                                                                                                                                                                                                  ttGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGtt        <  1:74212/239‑1 (MQ=255)                                                                                                                                                                                                                                       cAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTcc    <  1:915862/238‑1 (MQ=255)                                                                                                                                                                                                                                          gTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCCCTTTGTTAGCGGGGGCGGGATTCCAATGGTCGCGCCGGTCCACCGTTGCCACACCCCCCTTTGCACCACTCCGCGCTTTGTGGGTTTCCCt  >  2:726048/1‑237 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                             TTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGC‑GATCGCG‑CATTTGGTCAGGTCGC‑GGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCT  >  NC_000913/836393‑836860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑