Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 836,625 T→G 14.7% V92G (GTC→GGC)  ybiC → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913836,6250TG14.7% 162.7 / 3.4 70V92G (GTC→GGC) ybiCputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (11/45);  new base G (12/2);  total (23/47)
Fisher's exact test for biased strand distribution p-value = 7.85e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCT  >  NC_000913/836393‑836860
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            
tttATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTcgcg                                                                                                                                                                                                                                         <  2:142233/237‑1 (MQ=255)
   agtCAGTCTGTATTTCGTCAGATGGGTAGCGAGTAACAAGAAGCTAAATTAGTTGCCGATCATTTAATCGCGGCAAGCCAGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCGCGATGGCGATCGCGCATTTGGTCAGGTCGCGGcaca                                                                                                                                                                                                                                    <  2:49950/237‑1 (MQ=255)
   aTTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGcaca                                                                                                                                                                                                                                    <  2:738949/239‑1 (MQ=255)
   aTTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGcaca                                                                                                                                                                                                                                    <  2:505696/239‑1 (MQ=255)
    ttCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGGCGCGGCACAt                                                                                                                                                                                                                                   >  1:1149796/1‑239 (MQ=255)
     tCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATg                                                                                                                                                                                                                                  <  2:1149796/239‑1 (MQ=255)
         gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCCCCCCCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGc                                                                                                                                                                                                                              >  1:1199808/1‑239 (MQ=255)
         gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCCCGGCACATGAAGc                                                                                                                                                                                                                              >  1:1038776/1‑239 (MQ=255)
         gCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCACTTGCGATCGCGCTTTTGGTACAGTCGCGGGACATGaatc                                                                                                                                                                                                                              >  1:1058052/1‑237 (MQ=255)
            gTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGAt                                                                                                                                                                                                                           <  2:463047/239‑1 (MQ=255)
             tATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCCCATGAAGCGATg                                                                                                                                                                                                                          >  1:6982/1‑239 (MQ=255)
              aTTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATg                                                                                                                                                                                                                          <  2:90531/238‑1 (MQ=255)
                  cGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGTCACGCTCGCTGGCGAACCCGCCTTTTGGCAGGGCGCGGCACATGACGCGGTGgcgcc                                                                                                                                                                                                                     >  2:484789/1‑238 (MQ=255)
                       gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGTCACGCTCGATTGGGATCTCGCATTTGGGCAGGTCGCGGCCCACGAAACGATGGGGCCGGGGa                                                                                                                                                                                                                >  1:1093936/1‑239 (MQ=255)
                       gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGGGGGCCCGCCTTTGGTGAGGGGGCGGGCCATAAAGCGAGCGCGCTggggg                                                                                                                                                                                                                >  2:499975/1‑238 (MQ=255)
                       gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTggggg                                                                                                                                                                                                                >  1:643172/1‑238 (MQ=255)
                       gATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCCCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCCCCCCCCTGGCGAGCGCGCACTTGTGCACGGCGGGGCCCCTGGAGCGGTGGGGCCggggg                                                                                                                                                                                                                >  2:74212/1‑238 (MQ=255)
                            gTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCCCGCTCGGCGGCGGCACCCGCCTTGGGCCGGTCGCGGGGCCATGAGGCGCTGGCGCGGGGGATTgg                                                                                                                                                                                                            >  2:877542/1‑238 (MQ=255)
                                 gAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAg                                                                                                                                                                                                       <  2:551010/238‑1 (MQ=255)
                                  aGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCTCGATGGCGAACGCGCATTTTGGTAGGTCGCGGCACATGAAACGGTGGGGCTGGGGGTTggggaaag                                                                                                                                                                                                      >  1:850897/1‑231 (MQ=255)
                                  aGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCGCCTTTTGGCAGGGCGCGGGACATGAAGCGAGGGGGCCGGGGGTTGAGaaaagg                                                                                                                                                                                                     >  1:699259/1‑236 (MQ=255)
                                      actggagtcGAAAATAGTTGCCGATCTTTTAATCGCGGCAATCCTGTCAGCTCATGATTCACATGGTATTCGCATGATCCCAATCTATGTACAGTCCTCGAGTCAGGGGCACCTGGAAATCAACCTTCATGCGAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGGTCGATGGCGATCGCGCATTTGGTCAGGGCGCGGCATATGAAGCAATGGCGCTGGGGATTGAGAAAGCGCAt                                                                                                                                                                                                  <  2:1058052/230‑1 (MQ=255)
                                            agCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc                                                                                                                                                                                            <  2:553288/238‑1 (MQ=255)
                                            agCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg                                                                                                                                                                                           <  1:418498/239‑1 (MQ=255)
                                                                      tCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAAc                                                                                                                                                                 <  1:606073/239‑1 (MQ=255)
                                                                       cgcgGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACt                                                                                                                                                                <  2:1038776/239‑1 (MQ=255)
                                                                                         gCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGcc                                                                                                                                                 <  1:856714/236‑1 (MQ=255)
                                                                                                tCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTa                                                                                                                                       <  2:305889/239‑1 (MQ=255)
                                                                                                  acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                                                     <  1:415496/239‑1 (MQ=255)
                                                                                                  acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                                                     <  1:499975/239‑1 (MQ=255)
                                                                                                  acaTGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGAAGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACt                                                                                                                                     <  1:775059/239‑1 (MQ=255)
                                                                                                        tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAAc                                                                                                                                                                 <  1:206197/205‑1 (MQ=255)
                                                                                                        tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGc                                                                                                                                                             <  1:982297/209‑1 (MQ=255)
                                                                                                        tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCg                                                                                                                                <  1:854607/238‑1 (MQ=255)
                                                                                                        tATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCg                                                                                                                                <  2:643172/238‑1 (MQ=255)
                                                                                                          ttGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAg                                                                                                                             <  1:561357/239‑1 (MQ=255)
                                                                                                                  aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCCCGATGGCGATCGCGCATTTGGGCCGGGGGCGGCACCTGAAGCGAGGGGGCCGGGGGTTTAGAAAGGGCCTCAGCACGGGATTTCCGCCGGGGCGCGACATAACTCCCACCACAACGGCCGTATCGGGTGCTTGGGGGGGCAGtgtgg                                                                                                                     >  2:58087/1‑238 (MQ=255)
                                                                                                                  aTCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCAGCTCGATGGCGATCGCGCCTTTGGTCAGGGCGCGGGCCATGAAGAGGTGGCGCTGGGGATTGGGAAAGCGCATCAGCACGGTATTTGCGCCGTGGGGCTCCATTACACCCATCCTACAGGCCGTATCGGTTACTGGGGGGAGGAGggtgt                                                                                                                     >  2:764108/1‑238 (MQ=255)
                                                                                                                          cTAGGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGGCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCGACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGa                                                                                                                              <  1:534212/222‑1 (MQ=255)
                                                                                                                                    tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGTCACCCCCCATGGCGATCCCGCCTTTTGTCCGGTCGCGGCACCTGAAACCATGGGGGCGGGGGTTTAGGAAGGGCAACCACCCGGGGGTTGCGCCCGGGCGCCCCCTAACTCGCATCCTCTCGGCCGGATCGGGTTCTGGGGGGGGCCgtggggcgctgtcttttttacac                                                                                                    >  2:534212/1‑218 (MQ=255)
                                                                                                                                    tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGGGGGCACGCCCGATGGCGATCGCGCCTTTGGGCCGGGCGCGGGACATGAAGCGATGGCGCTGGGGGGTGAGAAAGCGCATCAGCACGGTATTGGCGCCGGGGCGCGACAATACTCGCATCCTATCGGGCGGGTCCGGTACTGGGGGGGGCCGGGGGCAGGGGCggggggtgtg                                                                                                     >  2:559497/1‑231 (MQ=255)
                                                                                                                                    tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGtctc                                                                                                   <  1:722284/239‑1 (MQ=255)
                                                                                                                                    tCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCCCGCCCCGAGGCGAACGCGCCTTTTGGTCGGGCGCGGGCCATGAAGCGGAGGGGCCGGGGGGTGGGAAAACGGCTCAGCACGGGGTTGGCGCCCGGGGGGTTCATAAACCGCCTCCTATCGGCCGGATCGGTTTCTGGGGGGGGCCGCGGGGAGCGggggggggtggg                                                                                                     >  2:669967/1‑231 (MQ=255)
                                                                                                                                           gTCAGGTGCACCTGCAAATTAACCATCATGCCAAACCCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                             <  2:371621/238‑1 (MQ=255)
                                                                                                                                           gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                             <  1:484789/238‑1 (MQ=255)
                                                                                                                                           gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCa                                                                                             <  1:764108/238‑1 (MQ=255)
                                                                                                                                              aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCGACGGCGAGCGCGCATTTTGCCAGGGCGCGGCACATGAAGCGAGGGCGCTGGGGATTGGGAAAGCGCCTCCGCACGGTAGTGACGCCGGGGCGCTGCCAAACTCCCATCATATCCGCCGCATCGGTTACTTGGGGGAGCCGGGGGGAGCGGCGGGGGTGGTCTCTATcacccat                                                                                         >  2:37490/1‑233 (MQ=255)
                                                                                                                                              aGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCAACCCCGATGGCGATCCCGCCTTTGGGCAGGGCCGGGCACATGAAACGATGGCGCGGGGGGGTGAGAAAACGCCGCAGCACCGGATTTCCGCCGGGGCGCTACATAAACCGCATCCAATCGGGCGGATAGGTTACGTGGCGGGGCAGAGTGGAGCGGCGGGGGTTTtctctctctcacct                                                                                         >  2:1115487/1‑233 (MQ=255)
                                                                                                                                                gggCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCCAACCCGCCTTTTGTCTGGGCCCGGCCCCTTAAACGGTGGGGCCGGGGGGTGAGAAAGGGCACTAGCCCCGGTTTGCCCCCGTGGCGCCACATAAATCGCAACCTCTTCGCCCGATCCGTTTCTGGGCGGGGGCGTGGGGCGCGGGGGGGGGTGtctttttttcctttct                                                                                       >  2:110097/1‑226 (MQ=255)
                                                                                                                                                          aaaTTAACCATCATGCCAAAACCGTTAAAGGGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTGGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:81468/238‑1 (MQ=255)
                                                                                                                                                          aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCTCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCTGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:100025/238‑1 (MQ=255)
                                                                                                                                                          aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  1:767429/238‑1 (MQ=255)
                                                                                                                                                          aaaTTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  1:747258/238‑1 (MQ=255)
                                                                                                                                                          aaaTTAACCATCAGGCAAAAACGGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:396679/238‑1 (MQ=255)
                                                                                                                                                          aaaTGAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGt                                                                              <  2:850897/238‑1 (MQ=255)
                                                                                                                                                             ttAACCATCATGCGAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGt                                                                          <  2:851714/239‑1 (MQ=255)
                                                                                                                                                                                  atAAAGAGGCGGGGGCGGCGGGCACGGTCGAGGGCGAGCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCACCAGTACGGTATTGCCGCCGTGGCGCTACATAACCCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGGGGCGGGGTTTGTCTCTATCCACTTTTTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTc                                                     <  2:545498/238‑1 (MQ=255)
                                                                                                                                                                                    aaaGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCa                                                   <  1:662848/239‑1 (MQ=255)
                                                                                                                                                                                                  ggcggTCCCGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCAGCAGCCCGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGc                                      <  2:699259/238‑1 (MQ=255)
                                                                                                                                                                                                  ggcggTCACTCTGGAGGGCGATCGCGCATTTGGGCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGc                                      <  2:466746/238‑1 (MQ=255)
                                                                                                                                                                                                        cACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCttt                                <  1:917839/238‑1 (MQ=255)
                                                                                                                                                                                                           gCTGGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACGCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                            <  1:669967/239‑1 (MQ=255)
                                                                                                                                                                                                           gCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAGGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCa                            <  1:877542/239‑1 (MQ=255)
                                                                                                                                                                                                           gCTAGAGGGCGATAGCGCATTTGGTCGGGTCGCGGCACATGAAGCGATGGGGCGGGGGAGTGAGAAAGCGCATCAGCACGGTGTTGCCGCCGTGGCGGTACATAACTAGGATCATATCGGCCGTATCGGTTTCGGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTAACCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGGTCCACGGTCGCGACAGCCGCTTTGGCa                            <  2:762298/239‑1 (MQ=255)
                                                                                                                                                                                                             tCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCAcc                          <  1:1088418/239‑1 (MQ=255)
                                                                                                                                                                                                                 tGGCGATCGCGCATTTGGTCAGGTCGCGGCCCATGAAGCGATGGCGCTGGGGATGGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAAt                       <  2:186340/238‑1 (MQ=255)
                                                                                                                                                                                                                                tttGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTTTCTATCCACTTTGTTATCGTGGGCGGGATTCCCATGGTCGCCCCGTTTCACGGTCGCGGCAGCCGCTTTGGCACCCATCCGGTCCGTGTggg         >  2:217365/1‑238 (MQ=255)
                                                                                                                                                                                                                                 ttGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGtt        <  1:74212/239‑1 (MQ=255)
                                                                                                                                                                                                                                      cAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTcc    <  1:915862/238‑1 (MQ=255)
                                                                                                                                                                                                                                         gTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCCCTTTGTTAGCGGGGGCGGGATTCCAATGGTCGCGCCGGTCCACCGTTGCCACACCCCCCTTTGCACCACTCCGCGCTTTGTGGGTTTCCCt  >  2:726048/1‑237 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            
TTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGAACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACGGTATTGCCGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCCGTTCTGTGTGGTTTTCCCT  >  NC_000913/836393‑836860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: