Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 353,610 C→G 18.7% A302G (GCG→GGG)  prpE → propionate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913353,6100CG18.7% 142.7 / 9.3 71A302G (GCG→GGG) prpEpropionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (25/32);  new base G (12/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 6.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATAC  >  NC_000913/353373‑353835
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    
gggTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGc                                                                                                                                                                                                                                      <  2:418146/238‑1 (MQ=255)
    tccGGGGGCGTGGCTGGAATCCAACGAAACCGCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCGGCTGGCGGGGATGGCGACt                                                                                                                                                                                                                                  <  2:1230757/237‑1 (MQ=255)
         tggcgtggcTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGt                                                                                                                                                                                                                           <  2:591367/238‑1 (MQ=255)
          ggcgtggcTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGGGGCGGCCCTCGttt                                                                                                                                                                                                                         >  2:1171926/1‑239 (MQ=255)
            cgtggcTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGAATATCGGTTAc                                                                                                                                                                                                                       >  1:1217163/1‑239 (MQ=255)
            cgtggcTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGGCCATCGGTTAc                                                                                                                                                                                                                       >  1:127673/1‑239 (MQ=255)
                         cAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGa                                                                                                                                                                                                          <  1:28679/239‑1 (MQ=255)
                               aaCCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTgg                                                                                                                                                                                                     <  1:1035599/238‑1 (MQ=255)
                                     ctGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCCATTCGTTTGCAAGGGTGTCCGACCTGGCCgggcg                                                                                                                                                                                              >  2:268894/1‑236 (MQ=255)
                                          ttCTCTCCACTCCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTgcggc                                                                                                                                                                                         <  2:1030778/239‑1 (MQ=255)
                                            ctctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACCAAGGATTGCCGACCTGGCCGGACTGCGGCgt                                                                                                                                                                                       >  1:250877/1‑239 (MQ=255)
                                             tctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTATCGTTTAAGCAGGATTGCCGACCTGGCCGGACTGCGGCgtg                                                                                                                                                                                      >  1:1053242/1‑239 (MQ=255)
                                             tctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTTCGAAGGATTGCCGACCTGGCCGGACTgcggcggg                                                                                                                                                                                      >  1:727930/1‑237 (MQ=255)
                                             tctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATTGCGACTATCGTTTTCGGAGGATTGCCGACCTGGCCGGGCTgcggcggg                                                                                                                                                                                      >  1:525130/1‑237 (MQ=255)
                                             tctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCgtg                                                                                                                                                                                      <  1:1170925/239‑1 (MQ=255)
                                             tctACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCgtg                                                                                                                                                                                      <  1:263084/239‑1 (MQ=255)
                                               tACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGAc                                                                                                                                                                                                                                   <  2:43658/194‑1 (MQ=255)
                                               tACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGAc                                                                                                                                                                                                                                   >  1:43658/1‑194 (MQ=255)
                                                     tCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCTATCGTTTCCGAAGGGTTGGCGGCCTGGCCGGACGTCGGCGTGTGGGGGAc                                                                                                                                                                              >  1:1126449/1‑239 (MQ=255)
                                                      ccGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGGTTTCCCACCCGGCCCGACTGGGGCGTGTGGTGGGCa                                                                                                                                                                             >  1:311622/1‑239 (MQ=255)
                                                       cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGACTACGGTTTCGGAGGGATGCCCACccggccgggcgggcgggggtggggggaaa                                                                                                                                                                            >  2:263084/1‑218 (MQ=255)
                                                       cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGGCTTCGTTTACTAAGGATTGCGAACCCGGGCCGACTgcggcggttgtggcggac                                                                                                                                                                            >  2:791545/1‑227 (MQ=255)
                                                       cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGGCTATCGTTTACGCAGGATTGCCGACCCGGCCGGGCTGCGGCGTGTGGTGGTCaa                                                                                                                                                                            >  1:975555/1‑239 (MQ=255)
                                                       cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGGGGGGCCTAACGGTTACCAAGGAGTGTCGCGCTTGGCGGGCTgcggcggggtgggggaaa                                                                                                                                                                            >  2:329412/1‑227 (MQ=255)
                                                       cGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGGTTACGAAGGATTGCCGACCTGGGCGGACTGCGGCGTGTGGTGGGCaa                                                                                                                                                                            >  1:942808/1‑239 (MQ=255)
                                                         gCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGGGTTCTTTTGCGCATCGGATATCGGCTGGGCGGTGGGGCATTTGTGTACCGTTTAGGCGCCGCTACTGGCGGGGGTGGGGGCTTTATGTTTGTGATGGTTGCCGACCTGGCCGGGAGgcggcggggggggggacatc                                                                                                                                                                          >  2:757896/1‑225 (MQ=255)
                                                               ccGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCga                                                                                                                                                                     <  2:489527/238‑1 (MQ=255)
                                                                cGGCAAACCTAAAGGCGGGCAGCGGGACGTCCGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCgaga                                                                                                                                                                   <  2:311622/239‑1 (MQ=255)
                                                                        cTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAAtcgt                                                                                                                                                                        <  1:211812/226‑1 (MQ=255)
                                                                         tAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAgg                                                                                                                                                          <  2:457148/239‑1 (MQ=255)
                                                                                 tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGGGGCTATCGTTTACGAAGGGTTGCCGACCTGGCCGGGCTGCGGCGTGTTGGGGGCAATCGTCGAGAAATATCCAGTTAGCTgg                                                                                                                                                  >  1:160641/1‑239 (MQ=255)
                                                                                 tGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGGGGGGGGCAATCGTCGAGAAATATCAGGTTAGcccg                                                                                                                                                  >  1:101088/1‑237 (MQ=255)
                                                                                  gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGGCTATCGTTTTCGTAGGATTGCCGCCCCCGCCGGGCTGGGGGGGGTGGGGGGCAAatgtcggggaaaaatctgtttcttat                                                                                                                                                  >  2:211812/1‑218 (MQ=255)
                                                                                  gCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGGGGCGGCTAACGGTTTCGAAGGATTGCCGACCCGGCCGGGGTTCGGCGGGGGGGGGGCAATCCGCGGGAAATAGCAGGttgaccgcg                                                                                                                                                 >  2:829248/1‑232 (MQ=255)
                                                                                        tGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGGGACTATCGTTTACGAAGGATTGCCGGCCTGGCCGGGCTGCGGGGGGGGGGGGGCAATTGTCGGGAAATAACAGGTTAGCCGGGTGGtct                                                                                                                                           >  1:45609/1‑239 (MQ=255)
                                                                                                              gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGGCCGCCATTcgctg                                                                                                                     >  1:1040967/1‑237 (MQ=255)
                                                                                                              gcgcTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACCATCGTTTACGCAGGGTTGCCGCCCTGGCCCGACTGCGGGGTGTGGGGGAGAATCGTGGGGAAATATCTGGTTAGGCGGATGTTCTCAGCGCCGACCGcccattgcgt                                                                                                                     >  2:1142402/1‑231 (MQ=255)
                                                                                                                        ccTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGGATATCGTTTACGGAGGGTTGCCCGCCTGGCCGGACTGCGGCGGGGGGTGGGCAATCTGCGGGAAATATCAGGGTTGCCCGGTGGTCTCATCGCCAGCCGCCCCTCGCGGGCTGagaaaa                                                                                                           >  2:983912/1‑239 (MQ=255)
                                                                                                                              tGGACACCATTTTGGGCGGCAAAGCGGGCAGCGTGTTCTTTGGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTccc                                                                                                      <  1:599680/238‑1 (MQ=255)
                                                                                                                                             gcggcAAGGCGGGCGGCGTGTTCTTTTGCGCATCGGATAGCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCg                                                                                       <  2:727930/238‑1 (MQ=255)
                                                                                                                                              cggcAAAGCGGGCGGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTCCGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGc                                                                                      <  1:2449/238‑1 (MQ=255)
                                                                                                                                               ggcAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTTCGGCGTGTGGGGGACAATCGTCGAGAAATATCAGGGTAGCCGGGTGTTCTCAGCGCCGACCGCCATTCGCGTGCTGCAAAAATTCCCTACCGCTGAAATTCGCaa                                                                                    >  1:901869/1‑239 (MQ=255)
                                                                                                                                               ggaaaaGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCa                                                                                     <  1:268894/235‑1 (MQ=255)
                                                                                                                                                           aGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGCGACTATCGTTTACGAAGGATTTCCGAACTGGGCGGGCTGCGGCGCGTGGGGGGCAATCGTCGGGAAATATCAGGTTAGCCGGGTGTTTTCTGCGCCGGCCGCCATTCGCGTGCTGAAAAAAATCCCTACCGCTGTAATTCGCAAACACGAtctct                                                                         >  2:448096/1‑238 (MQ=255)
                                                                                                                                                              gtgtTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGAtctctc                                                                        <  1:993544/236‑1 (MQ=255)
                                                                                                                                                                gtTCTTTTGCGCATCGGATGTCGGCGGGGTGGTGGTGCATTCGTATACCGTTTCCGCGCCGCTGCTGGCGGAGAGGGCGACTATCGTTTACGAAGGATTGCCGACCGGGCCGGACGGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATGCGCAAACACGATCTCTCGTCGc                                                                   <  2:1042108/239‑1 (MQ=255)
                                                                                                                                                                     tttGCGCATCGGTTATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTCCGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGaa                                                              <  2:1245928/239‑1 (MQ=255)
                                                                                                                                                                        gcgcATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTg                                                          <  1:1142402/239‑1 (MQ=255)
                                                                                                                                                                                 atagCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATct                                                  <  1:1171926/238‑1 (MQ=255)
                                                                                                                                                                                 atagCGGCTGGGTGGGGGGGCATTGGTATATCGTTTACGCGCCGCGGCGGGCGGGGAGGGCGACTATCGTTTACGAAGGATGGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTg                                                          <  1:866357/230‑1 (MQ=255)
                                                                                                                                                                                   atCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATctg                                                 >  1:942857/1‑237 (MQ=255)
                                                                                                                                                                                         tGGGTGGTGGGGCATTCGTATATCGTTTCCGCGCCGCTGCGGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGCCCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGgag                                          <  1:580851/238‑1 (MQ=255)
                                                                                                                                                                                          gggTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGGGGCGTGTGGGGGACAAACGTCGAGAAATATTAGGGTAACCGGGTGGTCTCAGCGCCGACCGCCATTCGCGTGCCGAAAAAATTTCCCAACCCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGGTGCCTATCTGGATGGGGaa                                        >  2:1248934/1‑239 (MQ=255)
                                                                                                                                                                                           ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGGGGGGTGGGCAAACGTCGAGAAATATCAGGGTAGCCGGGTGTTCTCAGCGCCGACCGCCATTCGCGGGGTGAAAAAATTCCCTACCCCTGAAATTTCCAAAAACGAACTCTCGTCGCTGGGAGGGGTCCAActggcctgtttta                                       >  2:866357/1‑231 (MQ=255)
                                                                                                                                                                                           ggtggtGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGGCCATCGTTTACGAAGGATTGCCGACCCGGCCGGACTGCGGCGTGGGGGGGGCAATCGTCGCGGAATATCAGGGTTGCCGGGTGGTCTCAGCGCCGACCCCCCTTTCCGTGCCGGAAAAATTTCCTACCGCTGAAATTCGCAAACACGGTCTCTCCGCGCTGGGCGTGCTCTCTCTGGGTGGACaa                                        >  2:813514/1‑238 (MQ=255)
                                                                                                                                                                                                tggGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGGGGGGGGCCATCCGCGAGAAATATCAGGGTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCCGAAATTCGCAAACACGATCTCTCGCCCCGGGGAGTGCTCTATCTGGCTGGGGAACCGccg                                  >  2:19622/1‑237 (MQ=255)
                                                                                                                                                                                                  gggCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGGTGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTgg                                 >  1:11297/1‑238 (MQ=255)
                                                                                                                                                                                                   ggCATTCGTATATCGTTTACGCGCCGCTGTGGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGa                                <  1:754165/238‑1 (MQ=255)
                                                                                                                                                                                                     cATTCGTTTATCGTTTACGCGCCGCTGCTGGGGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACg                              <  1:312168/238‑1 (MQ=255)
                                                                                                                                                                                                       ttCGTATATCGTTTACGCGCCGCTGCTGGCGGGGGTGGGGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGGGGTGGGCAATCGTCGAGAAATATCAGGGTAGCCGGATGTTCTCAGCGCCGACCGCCCTTTGCGTGCTGAAAAAATTCCCCACCGCTGAAATTCGCCAACACGAACTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAg                            >  2:877305/1‑238 (MQ=255)
                                                                                                                                                                                                          gTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTAt                                                    <  1:931127/211‑1 (MQ=255)
                                                                                                                                                                                                            atacCGTTGCCGCGCCGCTGCGGGGGGGTGGGGCGACGTTGGTTTACGAAGGATGGCCGCCCGGGCGGGCCGGCGGCGGGTGGGGGACAATCGCCGAGAAATACCGGGTTAGCCGGATGTTCCCGGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTCCCGCTGAAATTCGCAAACCCGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGAc                       <  2:45609/238‑1 (MQ=255)
                                                                                                                                                                                                                  tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCaa                                                                                    <  2:882167/172‑1 (MQ=255)
                                                                                                                                                                                                                  tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCaa                                                                                    >  1:882167/1‑172 (MQ=255)
                                                                                                                                                                                                                  tttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAAGATTGCCGACCTGGCCGGACCGCGGCGCGTGGTGGGCAATCGTCGGGAAAAATCAGGTTAGCCGGATGTTTTCAGCGCCGGCCGCCATTCCCGTGCTGGAAAAATTCCCCACCGCTGAAATTTGCCAACACCATCTCTCGTCCCTGGAAGTGCCCTTTATGGTTGGAGAACCGCGGGAGGAGGCGACCGCCCGt                >  2:596298/1‑239 (MQ=255)
                                                                                                                                                                                                                   ttACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGCCTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGt                <  1:1016906/238‑1 (MQ=255)
                                                                                                                                                                                                                     tcggggcgcTGGTGGCGGGGATGGCGACTATCGTTTACGAGGTATGCCCGGCCTGGCCGGACGGCGGCGTGTGGGGGACAATCGGCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg             <  2:901869/233‑1 (MQ=255)
                                                                                                                                                                                                                     aCGCGCCGCTGTGGGGGGGGAGGGGGACTTTCGTTTACGAAGGGTTGCCGACCGGGGCGGCCGGGGGCGTGGGGGGGACAATCGCCGAAAAAGAGCAGGTGAGCGGGATGTCCTCAGCGCCGCCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTgg             <  2:425855/239‑1 (MQ=255)
                                                                                                                                                                                                                      cgcgCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGGCGGGAAATATCCGGTTAGCCGGCTGTTCTCAGCGCCCACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAACCACCATCTCTCGTCGCTGGAAGTGCTCTATCTGGCCGGGGAACCCCCGGGCGGGCCGGCcccccggttgg            >  2:34401/1‑232 (MQ=255)
                                                                                                                                                                                                                            gcggctgGCGGGGAGGGCGACTAGCGTTTACGAAGGATTGCCGACCGGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCa      <  1:676130/236‑1 (MQ=255)
                                                                                                                                                                                                                                 tgGCGGGGAGGGGGACTACCGTTTACGAAGGTTTGCCGACTGGGCCGGATTGGGGCGGGTGGTGGACAACCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGCCCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATAc  <  2:942857/238‑1 (MQ=255)
                                                                                                                                                                                                                                 tgGCGGGGAGGGCGACTATCTTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATAc  <  1:1116787/238‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                    
GGGTACCGGTGGCGTGGCTGGAATCCAACGAAACCTCCTGCATTCTCTACACTTCCGGCACGACCGGCAAACCTAAAGGCGTGCAGCGTGACGTCGGCGGATATGCGGTGGCGCTGGCGACCTCGATGGACACCATTTTTGGCGGCAAAGCGGGCAGCGTGTTCTTTTGCGCATCGGATATCGGCTGGGTGGTGGGGCATTCGTATATCGTTTACGCGCCGCTGCTGGCGGGGATGGCGACTATCGTTTACGAAGGATTGCCGACCTGGCCGGACTGCGGCGTGTGGTGGACAATCGTCGAGAAATATCAGGTTAGCCGGATGTTCTCAGCGCCGACCGCCATTCGCGTGCTGAAAAAATTCCCTACCGCTGAAATTCGCAAACACGATCTCTCGTCGCTGGAAGTGCTCTATCTGGCTGGAGAACCGCTGGACGAGCCGACCGCCAGTTGGGTGAGCAATAC  >  NC_000913/353373‑353835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: