Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 989,959 | A→C | 17.6% | V133G (GTA→GGA) | pncB ← | nicotinate phosphoribosyltransferase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 989,959 | 0 | A | C | 17.6% | 128.5 / 4.1 | 75 | V133G (GTA→GGA) | pncB | nicotinate phosphoribosyltransferase |
Reads supporting (aligned to +/- strand): ref base A (34/24); new base C (2/12); total (36/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.86e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCACGAA > NC_000913/989723‑990196 | gggAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTAc < 1:992390/238‑1 (MQ=255) aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACCAATTTGC‑TTTCCAGCGTGTCGGAGGGCTGGGCC‑AAC‑TC‑GGCCTGCGGTGAGCGCTAGCGCTGTACCActt > 2:897698/1‑238 (MQ=255) aGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTAGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGGTTGCGC‑CAAGTC‑GGCCTGCGGGGAGCGAGAGCGATGTAcccattt > 1:148373/1‑234 (MQ=255) tCGTATTTGCTGGTGCCCACAAACCAGGATTCCTGTGGCAGA‑CGTTTAACTATGGTTTCTTTTACTACGAGAAAAAAGCGGCGACGGGTGCCAAAACCCATCAG‑ATGGAAGCGCGACATATCAAGCCCGGGGGTTAACGCCGAGAGGGCGACTAATTTGC‑TTCCCAGCGTGTC‑GAGGGTTGGCGC‑AACGCC‑GGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTG‑ATAACCG‑cc < 2:270343/237‑1 (MQ=255) gcggtggCCCACAAACCAGGATTCCGGTTGCAGA‑CGTTTAACGATGTTTTCTTTTACTTCGCGAAAAAAAGGGCGACGGGTCCCAAAATCCACCAG‑ATGGAAGCCCGACATACAAAGCCCGGCGTTTAACGCCGAGAAGTCGACTAATTTGC‑TTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGaa < 2:148373/233‑1 (MQ=255) cTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCCGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGGGATGGACCATTTTACCG‑ATAACCG‑CCAGCAAAAGa > 1:1209956/1‑237 (MQ=255) tGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GGGGGCTTGCGC‑CACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTTACTG‑ATAACCC‑CCCGCACAAGAACt > 2:428772/1‑239 (MQ=255) ggTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAAATTGC‑TTTCCAGCGTGTC‑GGGGGGGTGCGC‑AAACTC‑GGCCTGCGGGGGGCGATAGCGCTGTCGCAATTTACTG‑ATAAACGCCCACCAAGGGAACt > 2:44251/1‑239 (MQ=255) gCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGGACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTTACGCCGACAAATCGACTAATTTTC‑TTTCCCGCCTGGT‑GAGGGCTTAAGC‑AAAGTC‑CGCCTGCGGTGAGCGAAAAGGGTTGACCCTTTTCCTG‑AGACACG‑CCAGCAAAGGGAATTcgc > 2:649585/1‑237 (MQ=255) gCCCACAAACCAGGATTCCTGTGGCAGA‑CGCTTAACGATGTTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAAGCCATCAG‑ATGGAAGCGCGACATATCAAGCCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTcc < 1:1212552/238‑1 (MQ=255) caAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGC‑TTTTCAACGGGGC‑GAGGGCCTGCGC‑AACGCC‑GGCCCGCGGTGAGCGATAACGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCCGAgg > 1:136552/1‑239 (MQ=255) aaCCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCAAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTACCGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGAt < 1:428772/239‑1 (MQ=255) aCCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAAGTGACTAATTTGC‑TTTTCAACCGGTC‑GAGGGGCTGCGCAAACGTG‑GGCC‑GCGGGGGGTGGAAGAGGTGTACCATTTAACTG‑ATAACCC‑CCAGGAAAGAAACATCCCAGAGGaag > 2:934169/1‑237 (MQ=255) gATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTTCAACGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGGCCGCGGGGGGCGATAGCGGTTTACCATTTCACCG‑ATAAACG‑CCCGCAAAGGAAATTTCCCGAGGGTGACtt > 2:519363/1‑238 (MQ=255) aTTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTc < 1:484931/238‑1 (MQ=255) tttccgTTGCAGGCCGCTTAAGGATGGTTTCTTCTACGTCGCGAAAAAACCGGCGACGGGTGCAAAAAGCCATAAG‑ATGGAAGGCCGAAATATCAAGACCGGGGTTTAACGCCGAAAAGTCGATTAATTTGT‑TTCCCAGCGTGTC‑GAGGGCTTGCGC‑ACCGTC‑GGCCTGCGGTGGGCGATAGCGATGTCCCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTc < 2:784158/233‑1 (MQ=255) cTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTTC‑TTTTCAGCGGGTC‑GAGGGCTTGCGC‑AACGGC‑GGCCTGCGGTGAGCGGTAACGATGTAACATTTTACTT‑ATAAACG‑CCCGCAAAGGAACTTCCCCGAGGGTGACTTTACGc > 1:532900/1‑238 (MQ=255) cTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCCAGAAGTCGACTCATTTTC‑TTTCCCGCGGGTC‑GGGGGGTTGCGC‑AACGCC‑CGCCCGCGGGGGGGGGTAGCGGTGTACCATTTTCATG‑ATAAACC‑CCCGCAAAAGGACATCCCAGGGGGAGACtttccgc > 2:182688/1‑233 (MQ=255) ttttCTTGTACTGCGGAAGAAAAAGGGCGAGGGGTGCCAAAATCCA‑CAGAAGGGAAGCGCGACATATCAAGACGGGCGTTTAACGCGGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGA‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAatat < 1:967773/237‑1 (MQ=255) ttgtgcggcgaaagaAAAAGGGGGAGGGGTCCCAAAACCAAGAAA‑GGGGAGGCGCGAAATATCAAGACGGGCGGTAAACACCGAGAGGCCGAC‑AATTTGCTTTTCGAGCGTGTC‑GAGGGCTTGCAA‑AACGGC‑GGCCGGGGGTGAGCGATAGCGATGAACTATTCCACAG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCt < 2:1185405/227‑1 (MQ=255) tGTCCTTGGGGAAAAAAGCGGGGACGGGGCCAAAAACCCATCAG‑ATGGAAGCCGGACATATCAAGCCCGGCGGTTAACGCGGAGAAGTCGACTAATTGGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGA‑ACCGTC‑GGCCTGCGGGGAGCGATAGCGATGTCCCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCtt < 2:961553/239‑1 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTTAAGCCGAGAAGTCGACTAATTTGC‑TTTTCAGCGGGTC‑GAGGGGTTGCGC‑AACGGC‑GGCCCGCGGGGAGCGATAGCGGTGTACCATTTCACCG‑ATAACCC‑CCAGCAAAGGAACTTCCCAGAGGGTGACTTTACGCCCCGGGCCGCCTAAAcagaaatacagatttccctta > 2:1117046/1‑219 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGGGTC‑GGGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCATTTCAATG‑ATAACCG‑CCCGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGc > 1:1235500/1‑210 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCCGCGGTGGGCGATAGCGATGTAACATTTCACTG‑CTAAACG‑CCAGCAAAAGAACTTCCCCCAGGATGACTTCACGCCACCGGCCGCTTAAACGCATATCCAACTTTCCCttt > 1:1001106/1‑238 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGGACAACAAATTGC‑TTTACAGAGGGTCGGAGGGC‑TGCGC‑GAAATC‑GGGCTTCAGGGGGCGATAGACATTTCACATTTCCTCG‑CAAAACG‑CCCGCCAAGGGACTTACCAGAAGAAGGGTTCCAGCCACCGGGCGCCTAAACCGATATTCCACTTGCCACTa > 2:210005/1‑239 (MQ=255) aaccGGCGAGGGGGGCAAAAACCCATCAG‑ATGGAAGCGCGACATACCAAGACCGGCGGTAAACGCCGAGAAGTCGACTAATTTGC‑TTCCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGGGGTGAGCGATAGCGATGTCCCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGa < 1:296234/236‑1 (MQ=255) cgcgggcggtccccAAAACCCATCAG‑AGGGAACCGCAACATAGCAAGCCCGGCTGTTAACGCAGAAAGGTCGATTATTTTGT‑TTCCCAGCGGGTC‑GAGGCCTTGCGC‑AACGTC‑GGCCTGGGGTGAGCGATAGCGATGAACCATTTCACTG‑ATACCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGacac < 2:672391/227‑1 (MQ=255) gcaaaaacacATCAG‑ATGGAAGCGCGACATATCAAGCCCGGCGTTTAACGCCGAGAAGTCGACTAATTTGC‑TTCCCGGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCGGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 2:532900/230‑1 (MQ=255) gacAAAACCCATAAA‑GTGGAAGGCCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTCT‑TTTCCAGCGTGTC‑GGGGGTTGGCGC‑ACCGCC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCCCTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 2:849192/237‑1 (MQ=255) ccAAAATCCATCAG‑AGAAAAGCGGAAAATATAAGGACCGGCTGTTAACGCGAATAAGTCGACTATTTGGC‑TTCCCGGCGTGTG‑TGGGGCTTGCGC‑ACCGTG‑CGCCGGCGGTGGTCGAGAGCGAGTTCCCATTTCACTG‑ATACCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 2:943213/238‑1 (MQ=255) ccAAAACCCATCAG‑ATGGAAGCGCGACATATCAAGGCCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑tt < 1:519363/238‑1 (MQ=255) tCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCCTTTCACTG‑ATAACCG‑CCCGCCAAGGAACTTCCCAAGAGATGACTTCACGCCAACGGCCGCTTAAACGAAAATCCAGCTTGCCATTATCATTGGACACAGGGGCTTG‑TTCCGGGGTaaa > 2:572585/1‑238 (MQ=255) ggAAGCGCGAAAGATCAAGACCGGGGGTTAACGCCGAGAAGCCGACTAATTTGC‑TTCCCAGCGTGTC‑GAGGTCTTGCCC‑AACGTC‑GGCCGGGGGTGAGCAATAGCGATGTACCTTTCCCCTG‑AAAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGaa < 2:1209956/238‑1 (MQ=255) aaCATATCAAGCCCGGGGGTTAACGCCGGGAGGTCGACTATTTTGT‑TTCCCAGCGTGTC‑GGGGGTTTGCGC‑AACGGC‑GGCCTGCGGGGGGCGATAGCGATGTCCCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTaa < 2:289057/238‑1 (MQ=255) aCATATCAAGCCCGGCGGTTAACGCGGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCCCTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTaa < 1:44251/238‑1 (MQ=255) tatCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAAATTTC‑TTTCCACCGTGTC‑GAGGGCTTGCGC‑CACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCCTTTCACTG‑ATAACCG‑CCCGCAAACGCACTTCCCCGAGGATGACTTCCCCCCACCGGCCGCTTAAACGAATATCCCGCCTGCCCTTATTCTTGTACACGGTGGCTTGTTTCCGGGTTAAA‑CGGACAACGCGGAACCac > 2:689178/1‑238 (MQ=255) cAAGCCCGGGGTTTAACGTCGAAACGTCGGCTAGTTGGC‑TTCCCAGGGTGTG‑GAGGGTTTGCCC‑AAGGTC‑GGCGCGGGGGGACGGATAGCGTGGTCCCATTTCTCGA‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTa < 2:1226803/239‑1 (MQ=255) gggggTAAACGCCGGGAAGGCGAATAATTTGC‑TTTCCAGCTGGTC‑GAGGGCATGCCA‑ACCGTC‑GGCTGGCGGTGACGAATAGCGATGTACAATTGCCCTG‑AGAACCG‑CCAGCAAAGGAACTGCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCGTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 2:157560/236‑1 (MQ=255) gcggTTTAAGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGGGTC‑GGGGGCTTGCGC‑AACGTC‑GGCCTGCGGGGAGCGATAGCGATGTACCCTTTTACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCCCGGGCCGCTTAAACGAAAATCCAGCTTGCCATTATCGTTGGACACGGGGGATTG‑TTCCGGGGTAAAGCGGAACTCCGGGAAACAGTTaaaaaaaac > 2:686493/1‑232 (MQ=255) gcggTTAACGCCGAGAAGTCGACTAATTTTC‑TTTCCAGCGTGTC‑GGGGGCTTGCGC‑AACGGC‑GGCCTGCGGGGAGCGAGAGCGGTGTACCATTTTACTG‑ATAAACG‑CCCGCCAAGGAACTTCCCCGAGGGTGACTTTCCCCCACCGGCCGCTTAAACGAAAATACACCTTGCCCTTATCGTTTGACACGGTGACCTG‑TTTCGGGTTCAAGCGGAACTCCCGTAAACCGTTACGATAGt > 2:631283/1‑238 (MQ=255) cggTTACCGCCGAGAAGTCGACTAATTTGC‑TTCCCAGGGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGAGATTTCCCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc < 1:740722/238‑1 (MQ=255) cggTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCGGCGTTGC‑GTGTGCTTGCGA‑ACCGGC‑TGCGTGCGGGGATCGATAGCGATGTCCCATTTCCCTA‑AAAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc < 2:136552/238‑1 (MQ=255) agaAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaa > 2:379034/1‑239 (MQ=255) agaAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaa > 2:116645/1‑239 (MQ=255) aaGTCGACTAATTGGC‑TTTCCAGCGTGTC‑GGGGGCTTGCCA‑ACCGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg < 1:901034/239‑1 (MQ=255) agtcatagaaTTTGT‑TTCCCACTGTGCC‑GAGGGTTTGCGC‑CCCGTG‑GGCCTGCGGGGAGAGTTAGTGATGTACCATTGCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg < 1:1158402/230‑1 (MQ=255) gTCGGCTAATTTGC‑TTTCGTTGGTGGG‑GAGGTCTTGCGC‑ATGGCC‑GCCCTGCGTGGAGGGAGAGCGATGTCCTTTCTCATTG‑ATACCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggc < 2:379445/238‑1 (MQ=255) tCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcag > 2:1218794/1‑239 (MQ=255) aCTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggcagggc > 1:204556/1‑239 (MQ=255) ttttctttccaggcttcggggggTTTGCGC‑ACCGTC‑GCCCTGCGGTGCGCGATATCAATGGACCATTCCACTG‑AAAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGaaa < 1:1023389/219‑1 (MQ=255) tgcttccgggCGTGTG‑GGGGGCTTGCG‑‑AACGGCGGGCGAGCGGTGGGGGATGGCGGTGTTCCATTTCGCTA‑ATACCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGc < 2:20286/230‑1 (MQ=255) ttCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGcc > 1:707889/1‑236 (MQ=255) cAGTGTGTC‑GGGGGTTTCGGC‑AATCGC‑GGCCTGCGTGGGCCAATAGCTATGTACCATTTCACTT‑ATATCCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGat < 1:865714/239‑1 (MQ=255) gtgtC‑GAGGGTTTGCGC‑AACGCC‑GGCCGGCGGGGAGCGATAGCGATGTACAATTTCACAG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATAtt < 1:416752/238‑1 (MQ=255) gtggC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCCCTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATAtt < 1:1218794/238‑1 (MQ=255) tgtC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtca > 1:819315/1‑239 (MQ=255) tGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg > 1:731040/1‑239 (MQ=255) gcgc‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg > 1:1091819/1‑238 (MQ=255) gcgc‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg > 2:249316/1‑238 (MQ=255) gCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgct > 2:334793/1‑238 (MQ=255) gggcgtgaggggatagcgatGT‑CCCTTTCACCGAAAAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:548900/228‑1 (MQ=255) cggCGGTGAGCGATAGCGATGTCCCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:1152536/237‑1 (MQ=255) cgcgggggAGCGGAAGCGATTTAACTTTCCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:600998/232‑1 (MQ=255) cTGGGGTGAGCGATACCGTTGTGCCATTTCACTA‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:680435/239‑1 (MQ=255) cTGCGGTGGGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:182688/239‑1 (MQ=255) cTGCGGTGAGGGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgctgc < 1:1117046/238‑1 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc > 1:467246/1‑238 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc > 2:678394/1‑238 (MQ=255) agcgatagcgatGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaa > 1:490444/1‑175 (MQ=255) tagcgatGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGaa > 1:988364/1‑235 (MQ=255) cgatGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTc > 2:1020312/1‑239 (MQ=255) gttGTACCATTTCCCTG‑ACAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTc < 2:386943/236‑1 (MQ=255) atGTACCTTTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt < 1:747319/161‑1 (MQ=255) atGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCt > 2:747319/1‑161 (MQ=255) tACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCACGaa > 1:290137/1‑239 (MQ=255) aCCATTTCACTG‑AAAACCG‑CCATCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCACGaa < 2:669835/238‑1 (MQ=255) | GGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGA‑CGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAG‑ATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGC‑TTTCCAGCGTGTC‑GAGGGCTTGCGC‑AACGTC‑GGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTG‑ATAACCG‑CCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGCTTGCCATTATCGTTGGACACGGTGACTTG‑TTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTGAACCTGTTCACGAA > NC_000913/989723‑990196 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |