Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,151,689	G→T	30.3%	intergenic (+43/+22)	yegJ → / ← yegK	uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,689	0	G	T	30.3%	37.2 / 6.2	39	intergenic (+43/+22)	yegJ/yegK	uncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base T (7/5);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

AAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTC‑AGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151484‑2151924                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             aaactgagaagaGGGCAAGGGGGGAAAAAAAAAAAAAGAAATTTTTTC‑AGACGGGATGTATACGCGGGGGAAAAAAATTTACGGCGGTTATCCCTTTGATCTTTTATGGCC‑CCCCTACCAAAAAGAAGACG‑CCGAGGAGCTAAGAGCAAAATTAGTTCGTTAATCACCTGTTATATTTATTTTTAAAACGGGCGGTGG‑AT‑T‑TGTTG‑TCTACAACCGCCATGAGAAACTCACTCTGCCca                                                                                                                                                                                                            <  2:354739/233‑1 (MQ=255)  agagaaaaagagggCAAGGAG‑GGAAAAAAAAAAAAAGAGGTATTTCAAGATGGGTTGTATGGGCGGGGGAAAAAAATTTCGGGGGGTAAAACAATTAATCTTTTATTGG‑‑CCCCTTACCAAAAAAAAAAGCCCGAGGCGTTAAGAGAAAAATTATTTCTCAAACCAGCTTTTTTTTTTTTTTTTTAAGCGGGGGGTTG‑AT‑T‑TTTTT‑TCTACAACCGCCATGAGAAACTCACTCTGCCcac                                                                                                                                                                                                           <  2:153390/227‑1 (MQ=255)               gcaagaaGTGAAAAATCAAGAAAGAAAAATTTTC‑AAACTGATTGTATCGGCGGGGGAATAAAATTTACGGCGTTTATACCTTTGATCCTTTATTGGT‑CACTTATCAAAAAGAAGAAG‑CGGACGAGCAAAGACAAAAATTAGTTCGCTAATCGGCCGTTATTTTTTTTGTTAAACCTGGCGGTTA‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc                                                                                                                                                                                               <  1:329321/232‑1 (MQ=255)                                 aaaaagaaGATTTTGA‑AGAGGGGATGTATACCGGAGGGAATAAATTTTACGGCGGTTACA‑CTGTGATGCTTTTTTTGTCCACTTACCAAAAAGAAGAAG‑CCGACGAGCAAAGAGAAAAATTTGTTCGGAAATCAGCTTTTATTTTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                                                                                                            <  2:864813/237‑1 (MQ=255)                                                         aTGTATACGCGCGGGGAAAAATTTTACGGCGTTTATCCCATTGTTCTTTTATTGTT‑CACTTACCAAAAAAAAGAAG‑CCGACGAGCAAAGAGAAAAATTGGTTCGATACTCACCCTTTAT‑TTTTTTTTTTAAACCGGGCGTTG‑AATT‑TTTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTg                                                                                                                                                     <  1:1012976/239‑1 (MQ=255)                                                                                      cgtttATACATTTGATCTTTTATTGTT‑ACCCTATCAAAAAGAAAAAG‑CCGACAAATAAAGAAAAAAATAAGTCCGTTAACCACCCTTTATATTTATTGTTTAAAGTGGCGTTTA‑AT‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta                                                                                                                         <  1:567816/235‑1 (MQ=255)                                                                                           cacctaTTGATCTTTTTGTGGT‑CACCTAACAAAAAAAAGAAC‑CCGAGGAGAAAAGAGAAAAATTATTTCGCTAATCACCCGTTATATTTATTTTTAAAACTGGGGGTTG‑AT‑T‑TTTTG‑TCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAt                                                                                                                   <  2:704895/234‑1 (MQ=255)                                                                                                  tGATCCTTTATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTT‑TCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATTCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttg                                                                                                            >  1:905530/1‑239 (MQ=255)                                                                                                      ccTTTATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTT‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGGCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTAACAATTTAGGTAATAAATCGAgttgtt                                                                                                          >  1:357542/1‑237 (MQ=255)                                                                                                         ttATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TTTTT‑TCTTCAAACGCCCTGAGAAACTCACTCTTCCCACAGCCCCCGTGCCAACGTTTTTTCATCATCCGTACGCCCGTTTACCGCCCGGGTGGGCAAAAAATGCTTTAAAAATTCAAGGAATAAAACGAGTTTTTTCTGc                                                                                                     >  2:1032691/1‑239 (MQ=255)                                                                                                         ttATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTT‑TCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGGCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGTGCTGGACAAAAACTTCTTTAACAATTCAGGTCATAAATCGCACTGTTCCTGc                                                                                                     >  1:713692/1‑239 (MQ=255)                                                                                                         ttATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCCGGCGGGACAAAAAATGGTTTAACAAATCAGGGAATAAATCGAGTTGTTTCTGc                                                                                                     >  2:580730/1‑239 (MQ=255)                                                                                                         ttATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTTAAAATTCAGGTAATAAATCGAgttgttt                                                                                                         >  1:704895/1‑234 (MQ=255)                                                                                                               gT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TTTTT‑TTTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGTACGCTCGTTTACCGCCGGGGTCGGCAAAAACTGCTTTAACACTTCAGGTAATAAATCGAGGTGTTCCTGCGGtgct                                                                                               >  2:115312/1‑239 (MQ=255)                                                                                                                t‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TTTTG‑TCTTCAACCGCCATGAGAAAATCACTCTTCCCCCCACGCCCGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCGGGACAAAAAATTCTTTAACAATTTAGGGAATAAAACGAGTTTTTcccttgtttgt                                                                                               >  2:1095142/1‑229 (MQ=255)                                                                                                                t‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTTAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCggg                                                                                                                                                        >  1:811592/1‑181 (MQ=255)                                                                                                                        tCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTT‑TCTTCAAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAg                                                                                       >  1:198423/1‑239 (MQ=255)                                                                                                                               gaagaag‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TTTTG‑TCTTCAACCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCAGTCCAttt                                                                                >  2:1065740/1‑238 (MQ=255)                                                                                                                               gaagaag‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGCTTTACCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAAACGAATTGTTCCTTCGTTGCTGCCGCCCGTCCATTg                                                                                >  2:1037787/1‑239 (MQ=255)                                                                                                                                  gaag‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGGTG‑TCTTTAACCGCCATGAGAAACTCACTCTGCCCCCAACGCCAGTGCCAACGTTTTTTCATCATCCGTACCCTCGCTTACCGCCCGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATACATCGAGATGTTCCTCCGGTGCTGCCGCCAGTCCATTTaaa                                                                             >  2:612598/1‑239 (MQ=255)                                                                                                                                     g‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAAACGCCATGAGAAAATCAATCTTCCCACAACCCCCATGCCCACGGTTTATCATCATCCGCACGCCCGTTTACCCGCGGGGTGGGCAAAAACCGCTTTTACCAATCAGGTAATAAATTTAGTTGTTCCTGCGTTTCTTCCGCCACTCCCTTTaaaaaa                                                                          >  2:876101/1‑238 (MQ=255)                                                                                                                                       ccGACGAGCAAAGAGAAAAATTATCTCGCTAACCAGCCGTTATTTTTTTTTTTTAAACGGGCGGTTA‑AT‑T‑TGTTT‑TCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                           <  1:447540/237‑1 (MQ=255)                                                                                                                                       cagacgaGTAAAGAAAAAAATTATTGCGAAAATCACCGTTTATATTT‑TTTTTTAAACGGGGGGTTGAAA‑T‑TTTTG‑TCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                           <  1:92576/235‑1 (MQ=255)                                                                                                                                                       aaatTTAGTGCCTTACTCAGCTTTTATATTTATTTTTAAAACTGGCGGTTG‑AT‑T‑TTTTG‑CCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                                         <  1:1065740/239‑1 (MQ=255)                                                                                                                                                       aaaaTTAGTTCGATAACCAGCCTTTATATTTATTGTTAAACCTGCCGTTTG‑AA‑T‑TTTTG‑TCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                                         <  1:46992/239‑1 (MQ=255)                                                                                                                                                         aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTTCCCACAACGCCAGTGCCAACGTTTTATTATCATCCGTACGCTCGTTTAACCCCGGGCTGGACAAAAAATGCTTTAACAATTTAGGTAATAAAACGAGTTGTTCCTGCGTTGCTGCCGCCATTCCATTTAAAAACGGGGTAAAGAAAGGCACAt                                                       >  1:1045124/1‑239 (MQ=255)                                                                                                                                                           ttATTTCGATAATCGGCTGTT‑TTTTTTTTTTTTAAACGGGGGTTTG‑AA‑TGTGTT‑‑TTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                    <  1:868818/239‑1 (MQ=255)                                                                                                                                                            ttgTTCACTAATCACCCGTTATATTTTTTTGTAAACCGGGGGGTTA‑AT‑T‑TTTTG‑TCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                    <  1:454062/237‑1 (MQ=255)                                                                                                                                                                 ctctagcacgctgTTATTTTTTTTG‑TTAAACCGGGGGTTGAAA‑T‑TGTGT‑TCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                                               <  2:1209881/227‑1 (MQ=255)                                                                                                                                                                      cacAGCCTTTGTTTTTA‑TGTTTAAACCGGGGGTTT‑AATT‑TTTTT‑TCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                                          <  1:106962/237‑1 (MQ=255)                                                                                                                                                                                atatTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TTTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCCGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATAGAGATGTTTCTGCGTTGGTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATACAGCTAAttt                                >  1:327788/1‑239 (MQ=255)                                                                                                                                                                                     tattGTTTAAACTGGCGGTTT‑AA‑T‑TTTTT‑TCTTCAACCGCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCAACGTTTTATTATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAATCGAGTTGTTTCTGCGTTTGTGGCGCCAGTCCCTTGAAAAAAGGGGGAAAGAAAGGGACATGAGGGGAATTATACAGCAAATTTTACg                            >  2:103514/1‑238 (MQ=255)                                                                                                                                                                                      attGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCCGTGCCAACGTTTTATCATCATTCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCCGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGc                           >  1:1133651/1‑238 (MQ=255)                                                                                                                                                                                          tttAAACTGGCGGTTT‑AA‑T‑TGTTG‑TCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCCTCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAATCGCGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAAAGGGGTAAAGAAAGGCCCATGAGGAGAATTATCCAGCATATTTAACGCCAGt                       >  1:514188/1‑238 (MQ=255)                                                                                                                                                                                           ttAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAAAAATTCAGGTAATAAATCGAGTTGTTCCTGCGGTGCTGCCGCCAGTCCATTGAAAAAAGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTc                      >  1:966055/1‑238 (MQ=255)                                                                                                                                                                                            aaagcTGGCGGTTA‑AT‑T‑TGTTT‑TCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGc                    <  1:1095142/235‑1 (MQ=255)                                                                                                                                                                                             aaaCTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAGGCACCTGAGGAGAATTATCCAGCATATTTAACGCCAGTc                      >  1:265828/1‑236 (MQ=255)                                                                                                                                                                                                 gtggtgTTT‑AA‑T‑TGTTGTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCACCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGTTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                <  2:905530/234‑1 (MQ=255)                                                                                                                                                                                                  cgggtggtgaatt‑tgttt‑TCTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                <  1:1032691/227‑1 (MQ=255)                                                                                                                                                                                                    cGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc            >  1:481135/1‑239 (MQ=255)                                                                                                                                                                                                                    g‑tCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  2:760261/1‑236 (MQ=255)                                                                                                                                                                                                                    g‑tCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  1:637210/1‑236 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             AAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTC‑AGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGT‑CACCTATCCAAAAGAAGAAG‑CCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG‑AA‑T‑TGTTG‑TCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151484‑2151924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑