Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,151,689 G→T 30.3% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,151,6890GT30.3% 37.2 / 6.2 39intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (15/11);  new base T (7/5);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01

AAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151484‑2151924
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
aaactgagaagaGGGCAAGGGGGGAAAAAAAAAAAAAGAAATTTTTTCAGACGGGATGTATACGCGGGGGAAAAAAATTTACGGCGGTTATCCCTTTGATCTTTTATGGCCCCCCTACCAAAAAGAAGACGCCGAGGAGCTAAGAGCAAAATTAGTTCGTTAATCACCTGTTATATTTATTTTTAAAACGGGCGGTGGATTTGTTGTCTACAACCGCCATGAGAAACTCACTCTGCCca                                                                                                                                                                                                            <  2:354739/233‑1 (MQ=255)
 agagaaaaagagggCAAGGAGGGAAAAAAAAAAAAAGAGGTATTTCAAGATGGGTTGTATGGGCGGGGGAAAAAAATTTCGGGGGGTAAAACAATTAATCTTTTATTGGCCCCTTACCAAAAAAAAAAGCCCGAGGCGTTAAGAGAAAAATTATTTCTCAAACCAGCTTTTTTTTTTTTTTTTTAAGCGGGGGGTTGATTTTTTTTCTACAACCGCCATGAGAAACTCACTCTGCCcac                                                                                                                                                                                                           <  2:153390/227‑1 (MQ=255)
              gcaagaaGTGAAAAATCAAGAAAGAAAAATTTTCAAACTGATTGTATCGGCGGGGGAATAAAATTTACGGCGTTTATACCTTTGATCCTTTATTGGTCACTTATCAAAAAGAAGAAGCGGACGAGCAAAGACAAAAATTAGTTCGCTAATCGGCCGTTATTTTTTTTGTTAAACCTGGCGGTTAAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGcc                                                                                                                                                                                               <  1:329321/232‑1 (MQ=255)
                                aaaaagaaGATTTTGAAGAGGGGATGTATACCGGAGGGAATAAATTTTACGGCGGTTACACTGTGATGCTTTTTTTGTCCACTTACCAAAAAGAAGAAGCCGACGAGCAAAGAGAAAAATTTGTTCGGAAATCAGCTTTTATTTTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCg                                                                                                                                                                            <  2:864813/237‑1 (MQ=255)
                                                        aTGTATACGCGCGGGGAAAAATTTTACGGCGTTTATCCCATTGTTCTTTTATTGTTCACTTACCAAAAAAAAGAAGCCGACGAGCAAAGAGAAAAATTGGTTCGATACTCACCCTTTATTTTTTTTTTTAAACCGGGCGTTGAATTTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTg                                                                                                                                                     <  1:1012976/239‑1 (MQ=255)
                                                                                     cgtttATACATTTGATCTTTTATTGTTACCCTATCAAAAAGAAAAAGCCGACAAATAAAGAAAAAAATAAGTCCGTTAACCACCCTTTATATTTATTGTTTAAAGTGGCGTTTAATTTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGta                                                                                                                         <  1:567816/235‑1 (MQ=255)
                                                                                          cacctaTTGATCTTTTTGTGGTCACCTAACAAAAAAAAGAACCCGAGGAGAAAAGAGAAAAATTATTTCGCTAATCACCCGTTATATTTATTTTTAAAACTGGGGGTTGATTTTTTGTCTACAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAAt                                                                                                                   <  2:704895/234‑1 (MQ=255)
                                                                                                 tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATTCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAgttg                                                                                                            >  1:905530/1‑239 (MQ=255)
                                                                                                     ccTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGGCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAAATGCTTTAACAATTTAGGTAATAAATCGAgttgtt                                                                                                          >  1:357542/1‑237 (MQ=255)
                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTCTTCAAACGCCCTGAGAAACTCACTCTTCCCACAGCCCCCGTGCCAACGTTTTTTCATCATCCGTACGCCCGTTTACCGCCCGGGTGGGCAAAAAATGCTTTAAAAATTCAAGGAATAAAACGAGTTTTTTCTGc                                                                                                     >  2:1032691/1‑239 (MQ=255)
                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGGCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGTGCTGGACAAAAACTTCTTTAACAATTCAGGTCATAAATCGCACTGTTCCTGc                                                                                                     >  1:713692/1‑239 (MQ=255)
                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCCCCCGGCGGGACAAAAAATGGTTTAACAAATCAGGGAATAAATCGAGTTGTTTCTGc                                                                                                     >  2:580730/1‑239 (MQ=255)
                                                                                                        ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCCCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTTAAAATTCAGGTAATAAATCGAgttgttt                                                                                                         >  1:704895/1‑234 (MQ=255)
                                                                                                              gTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTTTTTTCAACCGCCCTGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATTATCATCCGTACGCTCGTTTACCGCCGGGGTCGGCAAAAACTGCTTTAACACTTCAGGTAATAAATCGAGGTGTTCCTGCGGtgct                                                                                               >  2:115312/1‑239 (MQ=255)
                                                                                                               tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTGTCTTCAACCGCCATGAGAAAATCACTCTTCCCCCCACGCCCGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCGGGACAAAAAATTCTTTAACAATTTAGGGAATAAAACGAGTTTTTcccttgtttgt                                                                                               >  2:1095142/1‑229 (MQ=255)
                                                                                                               tCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCCAAGTTTTATCATCATCCGTACGCTCGTTTACCGCCggg                                                                                                                                                        >  1:811592/1‑181 (MQ=255)
                                                                                                                       tCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTTTCTTCAAACGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGGCAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAg                                                                                       >  1:198423/1‑239 (MQ=255)
                                                                                                                              gaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTTTTGTCTTCAACCCCCATGAGAAACTCACTCTGCCCACAGCGCCAGGGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGGAATAAATCGAGTTGTTCCTGCGTTTCTGCCGCCAGTCCAttt                                                                                >  2:1065740/1‑238 (MQ=255)
                                                                                                                              gaagaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAAATCACTCTGCCCACAGCGCCAGTGCCAACGCTTTACCATCATCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTTACAATTCAGGTAATAAAACGAATTGTTCCTTCGTTGCTGCCGCCCGTCCATTg                                                                                >  2:1037787/1‑239 (MQ=255)
                                                                                                                                 gaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGGTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCCCAACGCCAGTGCCAACGTTTTTTCATCATCCGTACCCTCGCTTACCGCCCGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATACATCGAGATGTTCCTCCGGTGCTGCCGCCAGTCCATTTaaa                                                                             >  2:612598/1‑239 (MQ=255)
                                                                                                                                    gCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAAACGCCATGAGAAAATCAATCTTCCCACAACCCCCATGCCCACGGTTTATCATCATCCGCACGCCCGTTTACCCGCGGGGTGGGCAAAAACCGCTTTTACCAATCAGGTAATAAATTTAGTTGTTCCTGCGTTTCTTCCGCCACTCCCTTTaaaaaa                                                                          >  2:876101/1‑238 (MQ=255)
                                                                                                                                      ccGACGAGCAAAGAGAAAAATTATCTCGCTAACCAGCCGTTATTTTTTTTTTTTAAACGGGCGGTTAATTTGTTTTCTTCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                           <  1:447540/237‑1 (MQ=255)
                                                                                                                                      cagacgaGTAAAGAAAAAAATTATTGCGAAAATCACCGTTTATATTTTTTTTTAAACGGGGGGTTGAAATTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGaaaaa                                                                           <  1:92576/235‑1 (MQ=255)
                                                                                                                                                      aaatTTAGTGCCTTACTCAGCTTTTATATTTATTTTTAAAACTGGCGGTTGATTTTTTGCCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                                         <  1:1065740/239‑1 (MQ=255)
                                                                                                                                                      aaaaTTAGTTCGATAACCAGCCTTTATATTTATTGTTAAACCTGCCGTTTGAATTTTTGTCTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGcac                                                         <  1:46992/239‑1 (MQ=255)
                                                                                                                                                        aaTTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTTCCCACAACGCCAGTGCCAACGTTTTATTATCATCCGTACGCTCGTTTAACCCCGGGCTGGACAAAAAATGCTTTAACAATTTAGGTAATAAAACGAGTTGTTCCTGCGTTGCTGCCGCCATTCCATTTAAAAACGGGGTAAAGAAAGGCACAt                                                       >  1:1045124/1‑239 (MQ=255)
                                                                                                                                                          ttATTTCGATAATCGGCTGTTTTTTTTTTTTTTAAACGGGGGTTTGAATGTGTTTTTCCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                    <  1:868818/239‑1 (MQ=255)
                                                                                                                                                           ttgTTCACTAATCACCCGTTATATTTTTTTGTAAACCGGGGGGTTAATTTTTTGTCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag                                                    <  1:454062/237‑1 (MQ=255)
                                                                                                                                                                ctctagcacgctgTTATTTTTTTTGTTAAACCGGGGGTTGAAATTGTGTTCTCAACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGaa                                               <  2:1209881/227‑1 (MQ=255)
                                                                                                                                                                     cacAGCCTTTGTTTTTATGTTTAAACCGGGGGTTTAATTTTTTTTCTCAAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATc                                          <  1:106962/237‑1 (MQ=255)
                                                                                                                                                                               atatTTATTGTTTAAACTGGCGGTTGAATTTTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGTTTACCGCCCGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATAGAGATGTTTCTGCGTTGGTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATACAGCTAAttt                                >  1:327788/1‑239 (MQ=255)
                                                                                                                                                                                    tattGTTTAAACTGGCGGTTTAATTTTTTTCTTCAACCGCCATGAGAAAATCACTCTTCCCCCAGCGCCAGTGCCAACGTTTTATTATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAATCGAGTTGTTTCTGCGTTTGTGGCGCCAGTCCCTTGAAAAAAGGGGGAAAGAAAGGGACATGAGGGGAATTATACAGCAAATTTTACg                            >  2:103514/1‑238 (MQ=255)
                                                                                                                                                                                     attGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTTCCCACAGCGCCCGTGCCAACGTTTTATCATCATTCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCCGCGTTGCTGCCGCCAGTCCATTGAAAAAAGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGc                           >  1:1133651/1‑238 (MQ=255)
                                                                                                                                                                                         tttAAACTGGCGGTTTAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTTTCATCCTCCGTACGCCCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCCGGTAATAAATCGCGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAAAGGGGTAAAGAAAGGCCCATGAGGAGAATTATCCAGCATATTTAACGCCAGt                       >  1:514188/1‑238 (MQ=255)
                                                                                                                                                                                          ttAAACTGGCGGTTGAATTGTTGTCTTTAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCCACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAAATGCTTTAAAAATTCAGGTAATAAATCGAGTTGTTCCTGCGGTGCTGCCGCCAGTCCATTGAAAAAAGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTc                      >  1:966055/1‑238 (MQ=255)
                                                                                                                                                                                           aaagcTGGCGGTTAATTTGTTTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGc                    <  1:1095142/235‑1 (MQ=255)
                                                                                                                                                                                            aaaCTGGCGGTTGAATTGTTGTCTTCAACCGCCCTGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCCTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCCGTCCATTGAAAAACGGGGGAAAGAAAGGCACCTGAGGAGAATTATCCAGCATATTTAACGCCAGTc                      >  1:265828/1‑236 (MQ=255)
                                                                                                                                                                                                gtggtgTTTAATTGTTGTTCTCCACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCACCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGTTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                <  2:905530/234‑1 (MQ=255)
                                                                                                                                                                                                 cgggtggtgaatttgtttTCTACACCCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGa                <  1:1032691/227‑1 (MQ=255)
                                                                                                                                                                                                   cGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGGAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAcc            >  1:481135/1‑239 (MQ=255)
                                                                                                                                                                                                                   gtCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  2:760261/1‑236 (MQ=255)
                                                                                                                                                                                                                   gtCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAt  >  1:637210/1‑236 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            
AAACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGTTGTCTTCAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTACCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAAT  >  NC_000913/2151484‑2151924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: