Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,608,229 A→G 14.9% M439V (ATG→GTG)  hyfF → hydrogenase 4, membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,608,2290AG14.9% 236.9 / 8.9 120M439V (ATG→GTG) hyfFhydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (57/42);  new base G (17/3);  total (75/45)
Fisher's exact test for biased strand distribution p-value = 2.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

AATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCA  >  NC_000913/2607992‑2608465
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
aatctccTGCTCAAGTCCGTCCCGCGTGATCTCACCTTCGTTTGGAGGATGCTCAAAATCAGGCC‑TTTTTCCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGCCCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑t                                                                                                                                                                                                                                                  <  2:949621/233‑1 (MQ=255)
       ttcTCAAATACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTCCCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTgcg                                                                                                                                                                                                                                           <  1:1200046/237‑1 (MQ=255)
       tGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTgcg                                                                                                                                                                                                                                           <  1:675793/239‑1 (MQ=255)
       tGCTCAAGTACGGCACGCGCGACCTTAACGTGGTCTGTGGGATGCTCAAAATCATGCC‑ATGTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTgcg                                                                                                                                                                                                                                           <  1:747651/239‑1 (MQ=255)
         cTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TTGCTGcgcg                                                                                                                                                                                                                                         >  1:427375/1‑239 (MQ=255)
         cTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGcgcg                                                                                                                                                                                                                                         >  1:477811/1‑239 (MQ=255)
         cTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCCGGCAGGGATGCCG‑CCCATCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGCTTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGTCCTGGTTCGGG‑Tgggtcggcg                                                                                                                                                                                                                                         >  2:250545/1‑232 (MQ=255)
            aaGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGG‑TGGCTgcggcggg                                                                                                                                                                                                                                      >  1:633815/1‑234 (MQ=255)
              gTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGgg‑gt                                                                                                                                                                                                                                   >  1:986979/1‑239 (MQ=255)
                   gCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTCGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCCCCTGCTGATTATCGTCCCGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGTTACGGG‑GGGCTGCTCGGGT‑GGTTATg                                                                                                                                                                                                                              >  2:576122/1‑239 (MQ=255)
                   gCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATg                                                                                                                                                                                                                              >  1:1086747/1‑239 (MQ=255)
                   gCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGAAATCACCTGCTGATTATCGTCATGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCCGGTAAGGA‑GGgcggggcggggggtcatg                                                                                                                                                                                                                               >  1:245421/1‑231 (MQ=255)
                      cgcgcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCg                                                                                                                                                                                                                           >  1:819233/1‑239 (MQ=255)
                        cgcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGTCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGGTCT‑GGCGGGCCTGGTACGGG‑TGGCTGCGTGGGT‑GTTAATGGCGaa                                                                                                                                                                                                                         >  1:300529/1‑239 (MQ=255)
                                   cgtcgtCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGGTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTATGGA‑CAGGTCCGCGGGT‑GTTAATTGCGAAACCGCCG‑CA‑g                                                                                                                                                                                                             >  1:1143597/1‑238 (MQ=255)
                                     tcgtcTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGGGAAACCGCCG‑CA‑gggc                                                                                                                                                                                                          >  1:233469/1‑237 (MQ=255)
                                       gtcTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGGGGGG‑GGTAATTGCGGAACCGCCG‑CC‑GG‑GCGt                                                                                                                                                                                                       >  2:1211754/1‑239 (MQ=255)
                                       gtcTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGGACGGG‑TGGCTGGGCGGGT‑GTTTATGGCGAAACCGCCG‑CC‑GG‑GCgg                                                                                                                                                                                                       >  2:1242820/1‑238 (MQ=255)
                                       gtcTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGGACGGG‑AGGCTGCCGGGGG‑GTTAATGGGGGAAACGCCG‑CC‑GG‑GCgg                                                                                                                                                                                                       >  2:288091/1‑238 (MQ=255)
                                       gtcTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCGTGGACGGA‑TGGCTTCGCGGGG‑GTTAATGGCCAAACCGCCC‑CA‑GG‑CCGt                                                                                                                                                                                                       >  1:336791/1‑239 (MQ=255)
                                       gtcTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCCGGGACGGT‑TGGCTGGGCGGGG‑GTGTAAGGGGGAACCGCCC‑CG‑GG‑CCgg                                                                                                                                                                                                       >  2:71382/1‑238 (MQ=255)
                                       gtcTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGCTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGGCTGGTACGGG‑TGGGTTGGGGGGG‑GGTAATTGGGAAACCGCCG‑GA‑GG‑GCgg                                                                                                                                                                                                       >  2:240058/1‑238 (MQ=255)
                                             ggggTGGTCAAAAACCTGCCGTTTTCCCGCCGGGTTTTTTGGGGGCGGTGCGTTGGCGCGGGAGGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑ATTTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTAGCGTCCTGCGGTTATTGC‑TG‑TTAACGCTGGTGCG‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAAGGGAGAAACCGCCG‑CA‑GG‑CCGTTAAc                                                                                                                                                                                                   <  2:278945/238‑1 (MQ=255)
                                                atccccAAAATCATGCC‑TTTCGCCCCGGTGTGTTTTGGCGGAGGTGCGCTGGCGTTGGCGGGGA‑GCCGTCCTTTCAGTATTTTTCTAAGCG‑ATTTTATGCCCATT‑CCCGCGGGCCTGG‑CACGTAATCCCCTGCTGATTATCGTCCTGCTGTTATTGC‑TT‑TTACCGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCGGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCggg                                                                                                                                                                                               <  2:406010/233‑1 (MQ=255)
                                                 tgcctaaaatccgtcccttttCCCCCCGTGTTGTTGGGCGGCGGTGCGCGGGCGCGGGAGGGGAGGCCG‑CCTTCAAACTTTTTTTTTAGGG‑AATTTATGCCCTTT‑ACCGCCGGCCTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCG‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCggg                                                                                                                                                                                               <  2:754436/220‑1 (MQ=255)
                                                 tGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACCTTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCCGGTACGGG‑TGGCCGCGCGGGG‑GTTTATGGGGGAACCGCCGCCG‑GG‑CCGTTAGCCgggt                                                                                                                                                                                              >  2:1198116/1‑238 (MQ=255)
                                                  gCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGGGCGGGG‑GGTAATGGCGAAACCGCCG‑CC‑GGCCCGTAAACCggggg                                                                                                                                                                                             >  1:1002876/1‑238 (MQ=255)
                                                   ctaaaatTCATGCC‑TTTTCCCGCCGTGCTGTTTGGCGGGGGTGGGTTGGGGCTGGCAGGGGTGCCG‑CCCTCCAACTTTTTTCTTAGCG‑AATTTATAACCTTT‑CCCGCCGGCCTGG‑CACGTAACCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCGGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCGGCGGGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGa                                                                                                                                                                                           <  2:734721/236‑1 (MQ=255)
                                                   cTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGGCCGGTACGGA‑GGGGGGCGGGGGG‑GGTAATGGGGGAACCGCCGCCG‑GG‑CGGTGAACCgggggg                                                                                                                                                                                            >  2:181882/1‑237 (MQ=255)
                                                             tGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGGCTGGTAGGGG‑TGGGTGCGGGGGG‑GGTTATGGGGGAACCGCCC‑CC‑GG‑CCGGTAAACGGGGGGGTCTCGGCTgg                                                                                                                                                                                 >  2:473709/1‑239 (MQ=255)
                                                             tGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCCGGTACGGA‑TGGGTGCGCGGGG‑GTTAATTGCGGAACCGCCG‑CC‑GG‑CCGTTAACCGGGGGGATCTCGGCTgg                                                                                                                                                                                 >  1:871848/1‑239 (MQ=255)
                                                              gCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGTGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGt                                                                                                                                                                                >  1:973106/1‑239 (MQ=255)
                                                                   tttACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGGCCGGGACGGG‑TGGGGGCGCGGGT‑GTTAAAGGGGGAAACGCCG‑CAGGG‑CGGTTAACGgggggggctcgggggggttg                                                                                                                                                                              >  2:1034322/1‑222 (MQ=255)
                                                                   tttACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGGACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑ACGTTAACCGGGGGGATCTCGGCTGGGTGa                                                                                                                                                                             >  1:14398/1‑238 (MQ=255)
                                                                           cgTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑t                                                                                                                                                                                                                                                  <  2:768188/165‑1 (MQ=255)
                                                                           cgTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑t                                                                                                                                                                                                                                                  >  1:768188/1‑165 (MQ=255)
                                                                             tggtGTTTGGCGGCGGGGCGGTGGCGCTGGCGGGGATGCCG‑CCCTTCAACATTTTTCTTGGCA‑AATTTATGCCCATT‑CCCGCCGGACTGG‑CACGTAATCCCCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGcc                                                                                                                                                                   <  2:819233/235‑1 (MQ=255)
                                                                             tGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGGTAACCGGGGGGATCTCGGGTGGGTGACCACCTCGCCa                                                                                                                                                                  >  1:71053/1‑239 (MQ=255)
                                                                                                      gttggcGGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGCCCATT‑CCCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCatgatg                                                                                                                                          <  1:288091/236‑1 (MQ=255)
                                                                                                      gctggcAGGGATGCCG‑CCCTTAAATTTTTTTCATGGCG‑AATTTAGGCCCATT‑CCCGCCGGGCTGG‑CCCGAAACCCCCTGCTGATTTTCGTCCTGCTGTTATTGC‑TG‑TAAACGTTGGTGCG‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAAGGGCGAACCCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCatgatg                                                                                                                                          <  2:1086747/238‑1 (MQ=255)
                                                                                                       ctggcAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGGTGACCACCTCGCCAATGGTGATTCTGCTGGTCatgat                                                                                                                                           >  1:107026/1‑236 (MQ=255)
                                                                                                         ggcAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTg                                                                                                                                       >  1:1141717/1‑238 (MQ=255)
                                                                                                                  tgcgccccTCCAACATTTTCCTTAGCA‑ATTTTAGGACCAGT‑CCCGCCGGACGGG‑CACGTAATCCCCTGCTGATTATCGCCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCGGCGCGGGT‑GTTAATGGCGAACCCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATggg                                                                                                                               <  2:23312/233‑1 (MQ=255)
                                                                                                                   ccg‑ccCTTCAACATGTTTCTTAGCG‑AATTT‑GGAGCATTGCCCGCCGGCCTGG‑CCCGTAATCACCGTCTGATAATCGTCCTGCTGTTGTGTC‑TT‑TTAGCCCTGGGTCT‑GGCGGGCCTGGTCCGGA‑TGGCGCGGCGGTT‑GTTAATGGCAAACCCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATggg                                                                                                                               <  2:551107/236‑1 (MQ=255)
                                                                                                                    cg‑ccCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGG‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAAACGGGGGGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGGGATGGGAAc                                                                                                                            >  1:785071/1‑238 (MQ=255)
                                                                                                                       cctttaaaattttttCTTAGCG‑AGTTTTGGCCCATT‑ACCGCCGGACGGG‑CACGTAATCCCCGGCTGATGTTCGTCTTGCT‑TTTTTGG‑TGTTTAACGCTGGTGGT‑GGCGGGCTGGTTTCGGA‑TGGCTGCGCGGTT‑TTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCa                                                                                                                         <  2:336791/229‑1 (MQ=255)
                                                                                                                       cccTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGGTGACCACCTCGCCAATg                                                                                                                                                               >  1:652802/1‑201 (MQ=255)
                                                                                                                         cTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCata                                                                                                                       >  1:741607/1‑239 (MQ=255)
                                                                                                                         cTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTTCTGGTCATGATGCTGGCGGTGGGAACGCata                                                                                                                       >  1:23312/1‑239 (MQ=255)
                                                                                                                         cTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGGACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGGAACCGCCG‑CA‑GG‑CCGTTAACCCGGGTGATTTCGGCTGGTTGACCACCCCGCACATAGTGGTTCTGCTGGTCATGGTGCTGGCGACGGGGACGCa                                                                                                                         >  2:90959/1‑237 (MQ=255)
                                                                                                                               cATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCcac                                                                                                                 >  1:780198/1‑239 (MQ=255)
                                                                                                                                cattttCTTAGCGAAAGTTAGCACCATT‑CCGGCCGG‑CTGGCCACGTATCCCCCTGCTGTTTACCGTCCTGCTGTTATTGC‑GG‑TTAAGGTGGGGGCG‑GGGGGCCCGGGTACGGA‑TGCCTGCGGGGGT‑GTTAATGGCAAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCACGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCcac                                                                                                                 <  2:667756/237‑1 (MQ=255)
                                                                                                                                   tttCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CC‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGGTGGTCATGATGCTGGCGATGGGAACGCATATTTCACAAc                                                                                                              >  1:632646/1‑238 (MQ=255)
                                                                                                                                    ttCTTAGCG‑AATTTATGCCCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCGGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCt                                                                                                            <  1:1242820/239‑1 (MQ=255)
                                                                                                                                      cTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CC‑GG‑CCGTTAACCGGGGGGATCTGGGTGGGTTGCCCCCCCCCCCCATGGGGGTTCTGCTGGTCATGATGCTGGCGATGGGGACgacacttccacca                                                                                                               >  2:1151808/1‑222 (MQ=255)
                                                                                                                                                        ccttgcccgccgGACTGG‑CCCGTAATCTCCTGCTGATTCGCGTCCGGTTGTTAGTGC‑TG‑TGAACGCTGGTGCT‑GGCGGGCCTGGTCCGGG‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTggcg                                                                                          <  2:871848/233‑1 (MQ=255)
                                                                                                                                                          ttccccgcggGACTGG‑CCCGTATTCACCTGTGGCTTATCGTCCTGCTGTTTTTGT‑TG‑TTACCGCGGGTGCT‑GGGGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTggcggg                                                                                        <  2:985922/235‑1 (MQ=255)
                                                                                                                                                           ttcccgccgGGCTGG‑CGCGTAACCACCTGCTGATTATCGTCCGTGTTTTATTGC‑TG‑TTAACGCTGGGGCT‑GGCGGGCCGGGTCCGGA‑TGGCTGCGGGGGT‑GTAAAGGGCAAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTggcgggc                                                                                       <  2:1104614/236‑1 (MQ=255)
                                                                                                                                                           t‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTggcgggc                                                                                       >  1:507330/1‑238 (MQ=255)
                                                                                                                                                                  tgatcTGG‑AACGTAATCACCTGCTGTTTATCGTCTGGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCTTGTGCCGGA‑TGGCTGGGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCa                                                                               <  1:1112574/235‑1 (MQ=255)
                                                                                                                                                                    gACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGTCCACCTCGCCCATGGTGATTCTGCTGGTCCTGCTTCTGGCGGTGGGGACCCACATTCCACAACCTGTCATCCGGATCCTGGCGGGCGGCTTccc                                                                               >  2:1229263/1‑237 (MQ=255)
                                                                                                                                                                       gtg‑CACGTTATCACTTGCTGATTATCGTCGTGGTTTTATTGCATG‑AT‑GCGCGGGGGTT‑GGCGGGCCTGGT‑CGGA‑AGGGGTGGAGGGTGGTTAAGGGCAAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGG‑TCCTGGCGGGC‑TCTTCCACTATAg                                                                         <  2:812943/237‑1 (MQ=255)
                                                                                                                                                                       cgtgcgctttaccccatgcgtcttATCGTCGTGCTTTTATTGC‑TG‑TGAACGC‑GGTGCTGGGGGGGCCGGGTCCGGA‑GGGCTGCGGGGGT‑GTTAATGGCAAAGCCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACta                                                                            <  2:625722/216‑1 (MQ=255)
                                                                                                                                                                           cccttAATCACCTGCTGATTATGCCCCGTCTGTTTGTGC‑TG‑TTAACGTTGGGCGT‑GGCGGGCCTGGTACGGA‑GGGCTGCGCGGGT‑GTTAAGGCCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGt                                                                        <  2:507330/234‑1 (MQ=255)
                                                                                                                                                                           acgttAATCCCCGACTGTTTGTCGTCCTGCGGTTATGGC‑TG‑TTAACGCGGGGGCT‑GGCGGGCTGGGTACGGA‑TGGCTGCGGGGGT‑GTTAAGGGGAAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGt                                                                        <  2:633815/234‑1 (MQ=255)
                                                                                                                                                                                  tCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAg                                                                >  1:1088660/1‑239 (MQ=255)
                                                                                                                                                                                   cACCTGCTGTTTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCG‑GGCGGGCCTGGTACGGA‑TGGCGGCGCGGGT‑GTTAAGGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAgg                                                               <  1:130074/239‑1 (MQ=255)
                                                                                                                                                                                    aCCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGGTAACCGGGGGGATCTCGGCTGGTTGGCCACCCCGCCCATGGGGATTCTGCTGGTTATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGTGGGG‑GGTTCACCTATAGCCCCCTCAggg                                                              >  2:28727/1‑239 (MQ=255)
                                                                                                                                                                                        gctgATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGGTGGGTGTCCACCCCGCCCATGGGGGTTCTGCTGGTCATGTTGCTGGCGATGGGACCGCATATTCCTCAACCCGTCATCAGGAGCCTGGCGGGC‑GCCTCCCCTATAGTCCCCTCAcgggcc                                                           >  2:231551/1‑235 (MQ=255)
                                                                                                                                                                                        gctgATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGGTAACCGGGGTGGTCTCGGCTGGTTTACCACCCCCCCAATGGTGATTCTGCTGGGCATGATGCTGGCGGTGGGAACCCATATTTCACAACCTTTTCTCAGGATCCTGGCGGGG‑GGTTTCACTATAGTCCTCTCCGGGGCg                                                           >  2:556967/1‑238 (MQ=255)
                                                                                                                                                                                         ctgATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATTCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTTCTCTTAGGGACGc                                                          >  1:851776/1‑238 (MQ=255)
                                                                                                                                                                                            attacggcccTGTTGTTATTGC‑TG‑TTACCGCTGGTGTT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAAGGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACg                                                       <  1:282950/230‑1 (MQ=255)
                                                                                                                                                                                                tCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATct                                                   >  1:1071370/1‑238 (MQ=255)
                                                                                                                                                                                                tCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCCTGATGCTGGCGATGGGAACGCATATTCCACAACCTTTCATCAGGATCCTGGCGGGG‑GCTTCCACTATAGTCCTCTCAGGGACGCACCATcc                                                   >  2:393868/1‑237 (MQ=255)
                                                                                                                                                                                                tCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGGACGGG‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGTTGACCACCTCGCCACTTGGGATTCTGCTGGGCATGATGCTGGCGGTGGGGACGCCTATTTCACAACCTGTCATCCGGATCCTGGCGGGC‑GCGTCCACTATAGACCTCCCCAGGCCGCACgccct                                                   >  2:80228/1‑234 (MQ=255)
                                                                                                                                                                                                  gTCCTGCTTTTATTGC‑TG‑TTAACGCTGGGGCG‑GGCGGGCCGGGTACGGA‑TGGCTGGGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATctgc                                                 <  2:107026/238‑1 (MQ=255)
                                                                                                                                                                                                   tCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCCCTATAGTCCTCTCAGGGACGCCCGCTctg                                                  >  1:582995/1‑236 (MQ=255)
                                                                                                                                                                                                   tCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGTTGACCACCCCGCCCATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGGACGCCTATTCCCCAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACCATAGTCCTCTCACGGACGCACCAGctgcg                                                >  2:758994/1‑237 (MQ=255)
                                                                                                                                                                                                   tCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGGTGACCACCCCGCCAATGGGGATTCTGCTGGGCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCCTCCGGATCCTGGCGGGG‑GCTTCCCCTATAACCCTCTCAGGGCCGCCCGCTatgcct                                               >  2:957810/1‑239 (MQ=255)
                                                                                                                                                                                                   tCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGTTGACCACCCCGCCAATGGGGATTCTGCTGGTCATGATGCTGGCGGTGGGAACCCATATTCCACAACCCGTCCTCAGGATCCTGGGGGGG‑GCTTCCACCATAGTCCTCTCAGGGACGCACGACctgccc                                               >  2:940700/1‑238 (MQ=255)
                                                                                                                                                                                                      tgttgTTATTGC‑TT‑TAACCGCTGGTGCT‑GGCGGGCCTGGTGCGGG‑TGGCTGGGGGGGT‑GTTAATGGCAAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGca                                            <  2:751009/236‑1 (MQ=255)
                                                                                                                                                                                                      tgcttTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑TTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGca                                            <  1:957810/239‑1 (MQ=255)
                                                                                                                                                                                                      tgctgTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGG‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGGTAAACGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGGGATTCTGCTGGGCCTGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCGTCCACCATAGTCCTCTCAGGGACGCACGAGctgcctgc                                             >  2:648969/1‑238 (MQ=255)
                                                                                                                                                                                                      tgctgTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑Ca                                                                                                                                                                                                               >  2:80657/1‑79 (MQ=255)
                                                                                                                                                                                                      tgctgTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑Ca                                                                                                                                                                                                               <  1:80657/79‑1 (MQ=255)
                                                                                                                                                                                                      tgctgTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGca                                            <  1:473709/239‑1 (MQ=255)
                                                                                                                                                                                                      tgctgTTATTGC‑TG‑TTAACGCGGGTGCG‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAAGGGCGAACCCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGca                                            <  1:192108/239‑1 (MQ=255)
                                                                                                                                                                                                       gctgTTATGTC‑TG‑TTAGCGCTGGTGCG‑GGCGGGCCGGGTACGGA‑GGGCTGCGCGGGT‑GTTAAGGGGGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGca                                            <  2:427375/238‑1 (MQ=255)
                                                                                                                                                                                                        ctgTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGGCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGcaca                                          >  1:789368/1‑239 (MQ=255)
                                                                                                                                                                                                        ctgTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGGTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACCACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCAcc                                                                             >  1:889427/1‑203 (MQ=255)
                                                                                                                                                                                                        ctgTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACCGA‑TGGCCGTGCGGGT‑GTTAATGGCGGAACCGCCG‑CC‑GG‑CCGTTCACCGGGGTGATCTTGGCCGGTTGGCCCCCCCGCCAATGGGGATTCCTCTTGGCACGAAGCTGGCGATGGGCACGCAAATTTCACAACTCCTCATTACGAGCGTGGCGGGC‑CCTCCACCCATAGTCCTCTTCGGTGCGCACGTTCTGCTTCcaca                                          >  2:916746/1‑239 (MQ=255)
                                                                                                                                                                                                             aTTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑GGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTa                                     <  1:181882/239‑1 (MQ=255)
                                                                                                                                                                                                                   tG‑TTAACGCTGGTGCT‑GGCGGGCCTGGCACGGA‑TGGCTGCGCGGGG‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAAACGGGGTGATCTCGGTTGGTTGGCCACCTCGCCCATGGTGATTCTGTCGGTCATGATGCGGGCGATGGGAACGCATATTCCCCAACCCGTCATTACGAACCTGGCGGGA‑GCTTGCACTGTTGTCCCCTCAGGGACCGACGACctcgcggcaaacccacgcacc                                >  2:911797/1‑220 (MQ=255)
                                                                                                                                                                                                                    tgtaacggCTGGTGCT‑GGGGGGCGGGGT‑CGGAGTGGCTGGGGGGTT‑TTAAAGGGCGAACCCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCAcc                                <  2:1002876/232‑1 (MQ=255)
                                                                                                                                                                                                                        aaCGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGGGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTgg                             >  1:770113/1‑238 (MQ=255)
                                                                                                                                                                                                                              ggTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGAtt                      >  1:227244/1‑239 (MQ=255)
                                                                                                                                                                                                                                            gtGGGACGGA‑GGGCTGCGCGGGG‑GTAAAGGGGGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAgg         <  2:812636/238‑1 (MQ=255)
                                                                                                                                                                                                                                                     a‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCa  >  1:494897/1‑237 (MQ=255)
                                                                                                                                                                                                                                                     a‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCa  >  2:123243/1‑237 (MQ=255)
                                                                                                                                                                                                                                                     a‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCa  >  1:812636/1‑237 (MQ=255)
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AATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCC‑ATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCG‑CCCTTCAACATTTTTCTTAGCG‑AATTTATGACCATT‑ACCGCCGGACTGG‑CACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGC‑TG‑TTAACGCTGGTGCT‑GGCGGGCCTGGTACGGA‑TGGCTGCGCGGGT‑GTTAATGGCGAAACCGCCG‑CA‑GG‑CCGTTAACCGGGGTGATCTCGGCTGGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGGATCCTGGCGGGC‑GCTTCCACTATAGTCCTCTCAGGGACGCACGATCTGCCTGCACAACGTAGCACCTGGCATGATTTTTTGCCTTCAGGCACCGCA  >  NC_000913/2607992‑2608465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑