BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,451	C→T	5.1%	intergenic (‑179/+20)	argY ← / ← argV	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,451	0	C	T	5.1%	300.9 / 4.7	108	intergenic (‑179/+20)	argY/argV	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base C (43/58); new base T (4/2); total (47/61)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 108 total aligned reads displayed.

ACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCA  >  NC_000913/2818218‑2818686

aCCTCCGGCCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTg                                                                                                                                                                                                                                              <  1:108720/238‑1 (MQ=255)

  cTCCGACCGCTCGGTTCGTAGCCGAGTTCTCTATCCAGCTGGGCTACGGATGCATCGGGAAACTTTCTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaa‑a                                                                                                                                                                                                                                          <  1:1145759/239‑1 (MQ=255)

  cTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGaa‑a                                                                                                                                                                                                                                          <  2:953434/239‑1 (MQ=255)

    ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGt                                                                                                                                                                                                                                        >  1:338447/1‑239 (MQ=255)

    ccGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGt                                                                                                                                                                                                                                        <  1:502930/239‑1 (MQ=255)

      ggcggcgcGGTTCGGAGCAGAGTACACTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTAc                                                                                                                                                                                                                                      <  2:1233739/232‑1 (MQ=255)

      gACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTAc                                                                                                                                                                                                                                      <  2:338447/239‑1 (MQ=255)

         cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTa                                                                                                                                                                                                                                   <  1:587717/239‑1 (MQ=255)

              ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCCAAGTTATCCTCCCTGGGGCCTGCGGTGATTACCGCCCCATCTTCCATACCTTCATTGCTGAA‑AGTTCGTaaaa                                                                                                                                                                                                                                >  2:1215168/1‑237 (MQ=17)

              ggTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAata                                                                                                                                                                                                                              <  2:849490/239‑1 (MQ=255)

                  cGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGt                                                                                                                                                                                                                          <  1:163593/239‑1 (MQ=255)

                     aGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGCTTACTCGCCTTGCGGCTCGCCCTTCTGGCCGTTGCTAAAGCAACGTTTTCCTCCCTGGTGCCTTCGGTTGTTACCGCCACCTCTTCCATACCTTCATCGCTGCC‑AGTACGTAGAATATGGTG‑c                                                                                                                                                                                                                       >  2:836834/1‑238 (MQ=255)

                          aGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTTTCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCCCTTGA‑AGTAAGTAGGATTTGGTG‑CATCCgg                                                                                                                                                                                                                 >  1:483818/1‑239 (MQ=255)

                                        cTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCCGGCCGCTGCTCAAGCAACGTTATTCTCCCTGGGGCCTGCGGTTATTACCGCCCCCTCTTCCCTACCCTCATTGCTGCA‑AGGTCGTAGAATATGGTG‑TATCCCGCAGGGTTCGAAcc                                                                                                                                                                                                    >  2:594645/1‑238 (MQ=18)

                                           aGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTcc                                                                                                                                                                                                 >  2:234676/1‑238 (MQ=255)

                                           aGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCCAAAGCAACGTTTTTCTCCCTGGTACCCGCGGCTATTACCGCCCCCTCTTCCATACCTTCTTCGCTGAA‑AGTACGTAGAATCAGTGG‑CATACGGGCGGATTCTCACCTcc                                                                                                                                                                                                 >  2:100328/1‑238 (MQ=18)

                                            gCTACGGATGCACCGGGAAACTTAATTTGCTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCGCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCATGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCg                                                                                                                                                                                                <  2:420935/238‑1 (MQ=255)

                                             cTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCgc                                                                                                                                                                                               >  1:930927/1‑237 (MQ=255)

                                                 ggATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCGCCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                          <  1:940399/239‑1 (MQ=255)

                                                 ggATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                          <  1:1239816/239‑1 (MQ=255)

                                                 ggATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                          <  1:1189721/239‑1 (MQ=255)

                                                 ggATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                          <  1:49159/239‑1 (MQ=255)

                                                 ggATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                          <  1:99502/239‑1 (MQ=255)

                                                  gATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAGGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCt                                                                                                                                                                                          <  2:969702/238‑1 (MQ=255)

                                                       aTCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGGA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCGCGGt                                                                                                                                                                                      >  1:1188826/1‑237 (MQ=255)

                                                       aTCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGtt                                                                                                                                                                                     <  1:1184220/238‑1 (MQ=255)

                                                       aTCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGCGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGAACGTCGAATATTGTG‑CATCCCGGAGGATTCCAACCTCCGACCGCTCGGTTc                                                                                                                                                                                    >  1:1044478/1‑239 (MQ=255)

                                                          ggtcaattttctttactCCAGATTCTTTGATACCGATACTAAAGCCTTATCAAGTAAGAGATGGTGCATACGGTAGGATTACTCGCCTTGCGGCTCGCCCGTCGCGACGTTTCTACAGCCCCGTTCGCCGGCAGGGTGCGAGGGGTAATAGCCGCAGCTCCTTCCATCCCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                                                                                 <  2:682193/229‑1 (MQ=12)

                                                          gggAAACTGACGTTACTGCAGTGTGTTTGATACCGATACTAAAGACGTATCAAGGAAGAGATGGCGCATCAGGGAGGATTACTCGCCTTGCGGCTCGGCCGTCGGGCCGTTGCTAAAGCAGGGTTATCCTCCCTGGGGCCTGCGGTTATTACCGCCACCTCTCCCATCCCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                                                                                 <  2:445543/239‑1 (MQ=17)

                                                              aaCTTATTTTACTTTCGATTTTTTGATACCGCTGCTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGTTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCGCCTTTTCCATACCTTCATCGCTCAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCg                                                                                                                                                                             <  2:352490/239‑1 (MQ=17)

                                                               tttttctttccaGCAGATTCGTTGATTCCGCTATGAAAGCCGTAGCAAGGAAGAGATGGTGCATCCGGGACGATTCTTCGCCCTGCGGCTCGACCGTCGGATGGTTATTAAAGAAACTTTACACTCCTTGTGGCTGGCGGTTATTACCGCCCCTTCTGGCATCCCTTCATCCCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                                                                                                            <  2:964441/234‑1 (MQ=12)

                                                               acttacttTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGAGAGGATTACTCGCCTTGCGGCTCGCCTTTCGGTCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                                                                                                                                                                            <  2:590705/239‑1 (MQ=255)

                                                                  tatttttccgCAGATTTTTTGATACCGCTAATAAAGCCGTATCAAGTAAGAGATGGTGCAGCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑Ta                                                                                                                                                                        <  1:817375/230‑1 (MQ=255)

                                                                    cttttctgtaatttttttttTACCGCTACTAAAGCCGTATCAAGTAATGGATGGTCCATCCGGGTGGATTACTGCCCTTGCGGCTTGCCCTTCGGGCAGTTGCTAAAGCAACGTTATCTTCCCTGGTGCATGCTTTTATTACCGCTACCTTTGCCATACCTTCAACGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TAct                                                                                                                                                                      <  2:1113042/227‑1 (MQ=14)

                                                                    ctttactGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCATTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCGACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TAct                                                                                                                                                                      <  1:672992/239‑1 (MQ=255)

                                                                            cAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCa                                                                                                                                                               >  1:241790/1‑238 (MQ=255)

                                                                             agtttttttGATTCCTCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGATGATTTCTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTACCTTGGCTGGTGCAGGCGGGTATTAGCGACACCTCTGCCATACCTTCAACGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAg                                                                                                                                                              <  2:268657/235‑1 (MQ=17)

                                                                                   tttGATACCGCTACTAAAGCCGTATCAAGTAAGCGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTTGGGCCGTTCAGTAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTTATCCCGGTA‑AGTACGGAGAATATGGTG‑CATCCGGGAGGGTTCGAACCTCCCACTCCTCGGGTACTAGCGGCG‑TCTTCTAGCCAGCTGTGc                                                                                                                                                        >  2:118752/1‑238 (MQ=14)

                                                                                    ttGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTa                                                                                                                                                      <  2:930927/239‑1 (MQ=255)

                                                                                         aCCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTGTCCAGCTGAGCTACGGAt                                                                                                                                                 >  1:1144035/1‑239 (MQ=255)

                                                                                         aCCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCCGCTGAGCTACGGAt                                                                                                                                                 >  1:242672/1‑239 (MQ=255)

                                                                                           cGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGc                                                                                                                                               >  1:189904/1‑239 (MQ=255)

                                                                                             ctactaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGGG‑TACTCTATCCAGCTGAGCTACGGATGCaa                                                                                                                                             >  1:1098936/1‑239 (MQ=255)

                                                                                              tactaAAGCCTTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGGCTGCGGTTATTACCGCCTCCGCGTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCaaa                                                                                                                                            <  2:168204/239‑1 (MQ=255)

                                                                                                   aaGCCGTATCAAATAAGAGAAGGTGCAGCCGGGAGGATTACGCGCCTTGCGGATCGCCCTTCGGGCCGTTGTTAAAGTAACGTTATCCAGCAGGTGGCATACGGTTATTACCGCCACCTCGTCCATACGTTCATCTGTAAA‑AGTAAGAAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCg                                                                                                                                       <  1:200883/239‑1 (MQ=17)

                                                                                                        gTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGGTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGCTTCTAACCTCCGACCGCTCGGTTCGTAGGCGCG‑TACTCTATCCAGCTTCGCCACGGATTCACATGGCgggggg                                                                                                                                  >  2:711001/1‑235 (MQ=17)

                                                                                                          aTCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCCGGGGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGGA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGGTGCAAATGGCGGTGGGGc                                                                                                                                >  2:479079/1‑239 (MQ=255)

                                                                                                          aTCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTCGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCCAATGGCGGTGGGGc                                                                                                                                >  1:136431/1‑239 (MQ=255)

                                                                                                             aaGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCggg                                                                                                                             <  1:280902/239‑1 (MQ=255)

                                                                                                              aGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCCCCTCTTCCATCCCTTCATCGCTTAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCCAACCTCCGCCCGCCCGGCTCGTAGCCGGG‑GACTCTACCCAGCTGAGCCACGGATGCAAAACGCggggggggcggg                                                                                                                            >  2:178935/1‑229 (MQ=17)

                                                                                                               gTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCggggg                                                                                                                           >  1:333667/1‑238 (MQ=255)

                                                                                                                   gagaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTa                                                                                                                                                                                 <  1:977674/182‑1 (MQ=255)

                                                                                                                     gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGa                                                                                                                      <  1:1215168/238‑1 (MQ=255)

                                                                                                                       tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAA‑AGTACCTAGAATATGGTGCCTTCCGGGGGGTTTGAAACCCCCGA‑CGCCCGGTTCGTAGCCGAGTTACTC‑AACCCGCTGAACTACGGGTGCAAATGgggggggagggggggtttttat                                                                                                                    >  2:94621/1‑220 (MQ=2)

                                                                                                                        ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGGCGTTGCTAAAGCAACGTTATCCTCCCCGGGGCCTGCGGTTATTACCGCCCCCTCTTTCCTATCCTCATCCCTGAA‑AGTCCCTAGCATATGGTG‑CATTCGGGAGGCTTCGAACCTCCGACCGCTCGGCTCGTAGCCCAG‑TACTCTTTCCACCTCAGCTACGGATGCCAATGGGCGTGGGGCggggggtgtaggc                                                                                                                   >  2:205481/1‑229 (MQ=17)

                                                                                                                        ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAAcc                                                                                                                   <  2:977110/239‑1 (MQ=11)

                                                                                                                        ggTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGGTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGGTATTACCGCCCCCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCCGCTGAGCTACGGATGCAAATGGCGGTGAGGGGGGGGGTTGAAcc                                                                                                                   >  2:428221/1‑238 (MQ=17)

                                                                                                                             aTCAGGGATGATTACTGGCCTGCCGGCGCGTCCTTCCGGCCTTTCCTAAAGCACGGTTGTACTCCCTGG‑GTATGAGGTTATTCCCGCGCCCTGTCCAATCCCTTCATCGCTGAA‑ATTACGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATg                                                                                                            <  2:963302/239‑1 (MQ=17)

                                                                                                                              tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATg                                                                                                            <  1:546450/239‑1 (MQ=255)

                                                                                                                                    aggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACg                                                                                                                                                                                                                                     <  2:550792/114‑1 (MQ=255)

                                                                                                                                    aggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGGTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACg                                                                                                                                                                                                                                     >  1:550792/1‑114 (MQ=255)

                                                                                                                                       aTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCttt                                                                                                     <  1:1105306/237‑1 (MQ=255)

                                                                                                                                         tACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑Ac                                                                                                <  1:94621/239‑1 (MQ=255)

                                                                                                                                              gCCTTGCGGCTCGCCCTTCGTGCCGCTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCAACCGCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCAt                                                                                           <  1:594645/239‑1 (MQ=255)

                                                                                                                                              gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCAt                                                                                           <  1:234676/239‑1 (MQ=255)

                                                                                                                                              gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCAt                                                                                           <  1:1213870/239‑1 (MQ=255)

                                                                                                                                              gCCTGGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCAt                                                                                           <  1:946527/239‑1 (MQ=255)

                                                                                                                                               gcTTGCGGCTCGCCATCCGGGCCGTTGCTCAAGCAACGTTATCCTCCCTGGTGGTTGCGGTTATTACCGCCACCACTACCATCCCTTCAACGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATa                                                                                          <  1:1145201/238‑1 (MQ=21)

                                                                                                                                                cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCCCCTCTTCCCTACCTTCCTCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGCCCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGGGAGGCGGGGGTTCGAACACCCGATGCAGCttttttacgccta                                                                                           >  1:1239402/1‑229 (MQ=18)

                                                                                                                                                cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTGTCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATa                                                                                          <  2:1144035/238‑1 (MQ=255)

                                                                                                                                                 tgggggCACGCCTTTCGGGCCGTGGCTAAAGGAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACt                                                                                        <  2:483818/235‑1 (MQ=255)

                                                                                                                                                               tCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGa                                                                          <  2:333667/239‑1 (MQ=255)

                                                                                                                                                                 gggCTGTTGCTTAAGCAACGTTATCCTCCCTGTGGCCTGCGGTTATTACCGCAACTTCTTCCATCCCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAg                                                                         <  2:381350/238‑1 (MQ=255)

                                                                                                                                                                      gTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGTTTGCACCCCCGGCGGCGCCTTTG‑ACCCCCTACTCCCCTAGCCGGGGGGCGCCt                                                                    >  2:965016/1‑238 (MQ=18)

                                                                                                                                                                        tGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGTTTGAACCCCCGGGGGCGGTCTTG‑ACCGCATACCCCCCTAACGAGGGAGCGCCTTcg                                                                 >  2:858886/1‑238 (MQ=18)

                                                                                                                                                                                  aCGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTT‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCgg                                                       >  1:446905/1‑238 (MQ=255)

                                                                                                                                                                                  aCGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAAACCCCGAGTCAGCTTTTG‑ACCGCCTACTCCCTTAGCAGGGGAGGGGCTTCA‑GCCTCTCgg                                                       >  1:364414/1‑238 (MQ=255)

                                                                                                                                                                                    gTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAACCTTTG‑ACCCCATACCCCCCTAGAAGGGGGgcggcctccggctcaccggc                                                      >  2:567706/1‑221 (MQ=21)

                                                                                                                                                                                       aTCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGGACCCCCGATGCAGCTTTTG‑ACCCCCTACTCCCTTCACAGGGGGGCGCCCTCC‑ACCTCTCCGCCAcc                                                  >  2:589091/1‑238 (MQ=21)

                                                                                                                                                                                        tcctccCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGTGGGGGTTCGAGCCCCGGATGCAGCGTTTT‑ACCGCATACTCCCCTTGCAGGGGGGCGCCTTTA‑GCCTCTTGGCCACCt                                                 >  2:146740/1‑238 (MQ=21)

                                                                                                                                                                                        tcctccCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGTTCGAACCCCCGATGCAGCTTTTG‑ACCGCATACCCCCCTAGCAGGGGGGCGGCCTCA‑GCCCCCCgggccccc                                                 >  2:332379/1‑232 (MQ=21)

                                                                                                                                                                                            cccTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTcacca                                            <  2:189904/239‑1 (MQ=255)

                                                                                                                                                                                             ccTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGCGGCGGGGGTTCGAACCCCGGATGCCGCCTTTG‑ACACCACACTCCCCTAGCCGGGGCGCGCCCTCC‑GTCTCTCGGCCCCCTcccca                                            >  2:559061/1‑238 (MQ=21)

                                                                                                                                                                                             ccTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGTTCGAACCCCCGGTGCAGCTTTTT‑GCCGCCTTCCCCCTTTGCCGGGGAGCGCCTTCT‑GGCTCCTGGCCCCCCcaccc                                            >  2:498478/1‑237 (MQ=18)

                                                                                                                                                                                             ccTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGAGTCGAACCCCGGATGCCGCCTTTG‑ACCGCACACTCCCCTTGCAGGGGGGCGCCTTCA‑GCCTCTCCGGCCCCTccccc                                            >  2:743870/1‑237 (MQ=21)

                                                                                                                                                                                              ctgatgctggCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACcaca                                          <  1:984968/230‑1 (MQ=255)

                                                                                                                                                                                              cTGGTGCCTGCGGTTATTACCGCCGCCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACcaca                                          <  1:1128369/239‑1 (MQ=255)

                                                                                                                                                                                              cTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACcaca                                          <  1:332128/239‑1 (MQ=255)

                                                                                                                                                                                              cTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGAGTTTAACCCCCGATGCCGCTTCTT‑GCCGCCTACTCCCTCAGCAGGGGAGCGCCCTCA‑GCCCCTCGGCGATCTcccccc                                           >  2:209205/1‑236 (MQ=21)

                                                                                                                                                                                               tGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTTGCACGGGAGCGCCTTCA‑GCCTCTCGGCCAccccacccca                                          >  1:9066/1‑238 (MQ=255)

                                                                                                                                                                                                ggtgcgtacgcgtattaCCGCCGACTCTTCCAGACCCTCATATCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACCACACg                                        <  1:1085895/227‑1 (MQ=37)

                                                                                                                                                                                                ggTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACCACACg                                        <  1:858886/239‑1 (MQ=255)

                                                                                                                                                                                                  tGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATg                                                                                                                                                                                                                            >  1:491574/1‑61 (MQ=255)

                                                                                                                                                                                                  tGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATg                                                                                                                                                                                                                            <  2:491574/61‑1 (MQ=255)

                                                                                                                                                                                                       gCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGTTTGAACCCCGGCTGCAGCTTTTG‑ACCGCATACTCCCCTAGCAGGGGGGCGCCTTCA‑GCCCCTCGGCCACCCCACCCCACCCCTCtt                                  >  2:38640/1‑238 (MQ=25)

                                                                                                                                                                                                       gCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGGTTCGAACCCCGGATGCCGCCTTTGCACCCCTTAC‑CCCCTAGGATGGGAGAGCCATCACGCCTCTCGCCCCCCTC‑CCACCCGccccct                                  >  2:787588/1‑234 (MQ=18)

                                                                                                                                                                                                           ttattaCCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACCACACGCCTCTTACGa                              <  2:1239402/238‑1 (MQ=255)

                                                                                                                                                                                                                     cACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTg                                                                                                                                    <  2:303399/128‑1 (MQ=255)

                                                                                                                                                                                                                               aTACCTTCATTGCTGAAGATTTCGGAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGGAGAACTTGttt          >  1:1174604/1‑239 (MQ=255)

                                                                                                                                                                                                                                   cTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCCGCTTTTG‑ACCGCCAACTCCCCTAGCAGGGGGGCGCCCTCC‑GCCCCTCGGCCCCCTCACCACCCGCCTCTTTACAGGGCTTCGCAGAACTTGTTTTTcc      >  2:393125/1‑237 (MQ=25)

ACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTACTTTACTGCAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGTTATTACCGCCACCTCTTCCATACCTTCATCGCTGAA‑AGTACGTAGAATATGGTG‑CATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAG‑TACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTG‑ACCGCATACTCCCTTAGCAGGGGAGCGCCTTCA‑GCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCA  >  NC_000913/2818218‑2818686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`