Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,617,326 A→C 16.2% T105P (ACC→CCC)  nikD → nickel ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,617,3260AC16.2% 166.7 / 4.1 70T105P (ACC→CCC) nikDnickel ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (45/9);  new base C (2/13);  total (47/23)
Fisher's exact test for biased strand distribution p-value = 9.75e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGA  >  NC_000913/3617088‑3617564
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                
ccTGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGaaa                                                                                                                                                                                                                                                  <  2:535753/239‑1 (MQ=255)
ccTGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGaaa                                                                                                                                                                                                                                                  <  1:1091971/239‑1 (MQ=255)
  tGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCTGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCACCGCCCTTAATCCACTGCACACCATGCACACCCACCCGCGGGAAAc                                                                                                                                                                                                                                                 >  2:650057/1‑238 (MQ=255)
  tGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCCTGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCAAACCCCCGCGCGGAAAAc                                                                                                                                                                                                                                                 >  2:181880/1‑238 (MQ=255)
  tGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCCCGCGCGGGAAAc                                                                                                                                                                                                                                                 >  2:498429/1‑238 (MQ=255)
  tGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCATTTAATCCACTGCACACCATGCACACCCACGCCCGGGaaa                                                                                                                                                                                                                                                  >  1:1058007/1‑237 (MQ=255)
   gCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAAcc                                                                                                                                                                                                                                                >  1:1023834/1‑238 (MQ=255)
    cAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAAcct                                                                                                                                                                                                                                               >  1:47754/1‑238 (MQ=255)
    cAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAAcct                                                                                                                                                                                                                                               >  1:376108/1‑238 (MQ=255)
      aCGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAAcctgcc                                                                                                                                                                                                                                            <  2:141445/239‑1 (MQ=255)
          gggCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGc                                                                                                                                                                                                                                        >  1:1125799/1‑239 (MQ=255)
            gcgcGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCg                                                                                                                                                                                                                                       <  1:684914/238‑1 (MQ=255)
            gcgcGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCg                                                                                                                                                                                                                                       <  1:294728/238‑1 (MQ=255)
               cgTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAg                                                                                                                                                                                                                                   >  1:977216/1‑239 (MQ=255)
                                 cggTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGCTGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCCCACCCACGCGCGGGAAACCTGCCAGGGGTTAGGGAAACCCGCCGATGac                                                                                                                                                                                                                >  2:806514/1‑238 (MQ=255)
                                 cggTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCCGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGCAACCTGCCTGGCGTTAGGGAAACCCGCCGATGac                                                                                                                                                                                                                >  2:745023/1‑238 (MQ=255)
                                                          aCCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCCACGCTTACCGCTGCCATAGAAGc                                                                                                                                                                                      >  2:1198542/1‑239 (MQ=255)
                                                            cggcgcCGAAGCGGGGGGCTTGTGGCCGGCGGGGGTGCGCCGGGGGGGGGGGAAATTTTTGGCGGAGGGCAACCCGGTTCCGCCTGCCGCCCGGGGGGGCAAAAAAATTGCCACAATAAGGCAGACCCCGCGCCGCGCTTTAAACCCCCGGCACCCCAGGCCCCCCCCCGCGGGGGAACCCGGCGGGGGGTAGGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCgg                                                                                                                                                                                    <  2:35570/237‑1 (MQ=255)
                                                               cgccgcAACGCTGGGCCTTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAACTTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCGGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCAACGCGCGGGAACCCTGCCTGGAGTTAGGCAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGCTGCGGTgg                                                                                                                                                                                 >  2:828238/1‑239 (MQ=255)
                                                               cgccgcAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGCCGGCGGGGGAAATTTTAGCCGATGGCAAACCGGGTTAGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATACACTGCACACCATGCACACCCACACGCGGGAAACCTGCCTTGCATTAGGGAAGCCCGCCGGTTACGCGACGCATACCGGTGCCATAGACGCGGTgg                                                                                                                                                                                 >  2:346975/1‑239 (MQ=255)
                                                               cgccgcAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTgg                                                                                                                                                                                 >  1:310564/1‑239 (MQ=255)
                                                               cgccgcAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTgg                                                                                                                                                                                 >  1:115118/1‑239 (MQ=255)
                                                               cgccgcAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTgg                                                                                                                                                                                 >  1:278428/1‑239 (MQ=255)
                                                               cgccgcAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTgg                                                                                                                                                                                 >  1:1009965/1‑239 (MQ=255)
                                                               cgccgcAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGATAGGGAAACCCGCCGCTGACGCTACGCTTACCGCTGCCATAGAAGCGGTgg                                                                                                                                                                                 >  2:155341/1‑239 (MQ=255)
                                                                gccgcAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTggg                                                                                                                                                                                >  1:455831/1‑239 (MQ=255)
                                                                 ccgcAAGGGTGGGCAGTCGCCCCGGTGTGGTTCCCCAGGCGGCGGGAAAATGTGTACCCGAGGCCAAACCGTTTTCGCCTGGCTCCCTCCGCGGCATAAAAGCCGCCACCATCGTGCCACGCGCGCGAACCGCTTTTACCCCACGGCACCCCCGCCCCCCCCCCGCGCGGGAACCGCGCTGGGCTTAAGGGCACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTgggg                                                                                                                                                                               <  2:387780/239‑1 (MQ=255)
                                                                      aCGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGa                                                                                                                                                                          >  1:488725/1‑239 (MQ=255)
                                                                                tgggccccgcgggccgccGCCGGAGGGGGGGAAAAATTGAAGCAGATGACAACCGGGTTGCGCCTCGCCCCCGGCGGGGCACAAAATCTCCCCCCATCAAGAAGACCCCCGGCGGCGCTTTAACCCCCCTCCCCCCACCCCCCCCCCCCGCGGGGAAACCTGGCGGGGGTTAGGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCcgcg                                                                                                                                                                <  2:435691/222‑1 (MQ=255)
                                                                                  gtgccggtggtggcgcgcagggcggcggGGGAACTCTACGCCTAGGCCAACCCTGTTGCGCCCTGCCCCGGGGTGGCATTCAACCATGCCACGATCAGGCACCGCCCGGGCAGCGCGTTTACCCCCCCGCCACCCATCCCCCCCCACCGGCGGAAGACCTCCGGGGGGTTGGGGACGCCCGCCGATGACGCTACGCTTACCGCTGCCATTGAAGCGGTGGGGCTGGAAAACGCcgcgcg                                                                                                                                                              <  2:800484/217‑1 (MQ=255)
                                                                                         tgggCGTCCCCGAGCCGGGGGGGGAATTTTAAGCCGAGGGAACCCCGTTGCCGCCTTCCGCCCGGCCGGACAAAAAACTTCCCCCCACCAGGCAGACCCCGCGCGGCGCCTTTAACCCCCTCACCCCCATCCCCACCCCCGCGGGGGAACCCGGGCGGGTGTGAGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCt                                                                                                                                                        <  2:773118/236‑1 (MQ=255)
                                                                                         gtggtctccgcacggcgggggGGGAATTTTGAGCCGAGGCAAAACCGGTTCCCCCTTCGCCCCTGCGCGCCAAAAAATTTCCCACAATCCGGCAAACCCGCCGCAGCCCTTTTAACCCCCCGCACCCCACCCACCCCCCGGCGGGGAAAACCTGCCGGGCGTGGGGGAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCt                                                                                                                                                        <  2:1190146/223‑1 (MQ=255)
                                                                                         gggggctgcGCCAGGGGGGGGGGGAAATTTTGCCGGAGGCCACGCCGTTTCCCCCTGCCGCCCTGGGCGGCAAAAAAACCCCCACCACCAAGAAACCCCCGCGCAGCGCTTTTACCCCCCCGCCCCCCACCCACCCCCCCGGGGGGAAACCCTGCGGGGCTTGAGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCt                                                                                                                                                        <  2:1205254/230‑1 (MQ=255)
                                                                                              agtccgccggcgggggGGGAAAATTTTGGCGGAGGCAAACCCGGTTCGCCTTGCCGCCCGGCGCGCCACCAAAATCCCCCCCACCATCCAGACCCCGGCCGCCGCTTTTACCCCCCTCCCCCCCACCCCCCCCCCCGCGGGGAAACCCGGCGGGGGGTAGGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAg                                                                                                                                                    <  2:579496/228‑1 (MQ=255)
                                                                                                       aCGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAACCGCCGCGCGCGTGCGGAAGCTGTACCCGt                                                                                                                                          >  2:495435/1‑238 (MQ=255)
                                                                                                        cggcggGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTc                                                                                                                                        >  1:640049/1‑239 (MQ=255)
                                                                                                           cggGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCggg                                                                                                                                     >  2:208513/1‑237 (MQ=255)
                                                                                                                                 caatcgcgTTTCCCCGCGCCCCCTGCGGGCCACAAAAATGCCCACCATCATACAGACCCCGCGCCGCCCTTTTAACCCACTACACACCACCCACCCCCCCGCGCGGGAACCCGGCGTGGGGTTGGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAg                                                                                                                <  2:1125799/231‑1 (MQ=255)
                                                                                                                                    tgtgcagtggattaaaggcgcTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAgcgc                                                                                                             >  1:868018/21‑239 (MQ=255)
                                                                                                                                               cctccccccgtGCGGGACAACAATATCGCCAACACCGTGAAGACCCCGCGCCCCCCTTTACACCCCCCGCACACCACCCCCCCCCACGCGGGGGAACCCTGCCGGGTGTGAGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGc                                                                                                  <  2:959593/229‑1 (MQ=255)
                                                                                                                                                      cccTCCGCGACAAAAAAACTCCCCCCACCATGCCGAACCCGCGCAGCGCCTTTCACCCACTCCCCCCCATGCCCCCCCCCGCGCGGAAACCCTGCCTGGGTTGAGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCg                                                                                           <  2:242118/239‑1 (MQ=255)
                                                                                                                                                        cTGCGCCGCATCAAAATTGCGACCATCATCCAGAACCCGCTCAGCGTCTTTAATCCAGTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAACCGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGATGCAGCGCATGATGATTGCGATGGCGGt                                                                                         >  2:516712/1‑239 (MQ=255)
                                                                                                                                                         tGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGCTGAGCGGCGGCATGTTGCAGCGCCTGATGATTGCGATGGCggg                                                                                         >  2:81153/1‑237 (MQ=255)
                                                                                                                                                         tGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGt                                                                                         >  1:339949/1‑238 (MQ=255)
                                                                                                                                                          gcgcGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGc                                                                                       >  2:807719/1‑239 (MQ=255)
                                                                                                                                                               ggtataaaaaTACCCTCCATAACGCAGAACGCGCGCAGCGCCTTTACTCCACCGCCACCCACGCCCCCCCGCGGGGGGAAACCCTGCCGGGCGGTGGGAAACACCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCtgt                                                                                    <  2:636385/232‑1 (MQ=255)
                                                                                                                                                                    cAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGaa                                                                               >  1:398779/1‑238 (MQ=255)
                                                                                                                                                                     caaactccccaccaTCGTGCAGACCCCGCGCAGCCCTTTTACCCCCCCCCCCCCCCCCCCCGCCCCCGCGGGGAAACCCTGCCTGGGGTAAGGGAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAAt                                                                              <  2:1223167/231‑1 (MQ=255)
                                                                                                                                                                            gCCACCATCATGCAGAACTCCCGCAGCGCCTTTAATCCACTGCCCACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCACAGCAGCGGTGGGGCCGGAAAAAGCCGCGCGCGTGCTGATGCCGTACCAGTTCGAGCTGTGCTGCGGCATGTTGAAGCGCATGATCATTGTGATGGCGGTGCTGTGTGAATCTcctgt                                                                       >  2:766341/1‑236 (MQ=255)
                                                                                                                                                                                             cccGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGGTGATTGCGATGTCGGTGCTGTGGGAATTCCCGTTTATCATCCCCGATTaa                                                       >  2:249302/1‑238 (MQ=255)
                                                                                                                                                                                              ccGCCCAGCGCCTTAAACCCCCCGCCCCCCTACCACCCCCCCGCGGGGGAACCCGGCCGGGGGTTGGGAAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAacc                                                     <  1:503346/239‑1 (MQ=255)
                                                                                                                                                                                                gcgcAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCAGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccg                                                    >  1:1209450/1‑238 (MQ=255)
                                                                                                                                                                                                  cgcAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccga                                                   >  2:1047640/1‑238 (MQ=255)
                                                                                                                                                                                                  cgcAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccga                                                   >  2:653467/1‑238 (MQ=255)
                                                                                                                                                                                                     gccgcttaaaaTCCCCTGCACACCAGTCCCCCCCCCGCGGGGAAAGCCTGCCGGGCGTTAGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccac                                               <  1:806514/230‑1 (MQ=255)
                                                                                                                                                                                                     gccgcCTTTACTCCCTGGCACACCATGCACACCCACGCGCGGGAACCCGGCAGGGTGTTAGGAAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAaccgaccac                                               <  1:960655/237‑1 (MQ=255)
                                                                                                                                                                                                         ccTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGGTGATTGCGATGGCGGTGCTGTGGGAATCACCGTTTACCATGGCCGATGAaccgaccaccga                                            >  2:100968/1‑238 (MQ=255)
                                                                                                                                                                                                                     cTGCACACCATGCACACCGACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGGGCTGAAGCTGAACACGTTCGAGATGAGCGGCGGCATGTTGCAGGGCATGATGATTGCGATGGCGGTGCTGAGTGGAGCACCGTTAATCACCTCCGATGGaccgaccaccggcatttgcggcgt                                >  2:842906/1‑226 (MQ=255)
                                                                                                                                                                                                                     cTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGCGTGAATCACCGTTTATCATCCCCGTTGAGCCGACCACCCCCCCTGGCGTGGt                                >  2:947106/1‑239 (MQ=255)
                                                                                                                                                                                                                       gCACCCCATCCCCCCCCAGGCGGGGAACCCCTGCCTGGCGTTGGGGAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAg                              <  1:199221/239‑1 (MQ=255)
                                                                                                                                                                                                                       gCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTa                               >  2:712454/1‑238 (MQ=255)
                                                                                                                                                                                                                           accccaccccccccgccggggggAAACCGGGCGGGGGGTGGGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGca                            <  2:977216/219‑1 (MQ=255)
                                                                                                                                                                                                                           acCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGcaca                          >  1:901436/1‑239 (MQ=255)
                                                                                                                                                                                                                               tGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGCACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGAGGAACCGCCCACCGACCTTGACGGGGTAGCACGGgc                       >  2:137208/1‑238 (MQ=255)
                                                                                                                                                                                                                                gCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTa                               >  1:1081045/1‑229 (MQ=255)
                                                                                                                                                                                                                                 ccccccccCGCGCGGGAACCCGGCGGGGGGTAAGGAAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGgcgcg                    <  1:650057/235‑1 (MQ=255)
                                                                                                                                                                                                                                    acCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCa                  >  2:280613/1‑238 (MQ=255)
                                                                                                                                                                                                                                    acCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGAGGAACCGACCACCGACCTTGCCGTGGTAGGACAGgcgccgc                  >  2:1203635/1‑235 (MQ=255)
                                                                                                                                                                                                                                     ccccccggcggggAACCCTGCGGGGCGTGGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCAt                 <  1:1039830/229‑1 (MQ=255)
                                                                                                                                                                                                                                     cccACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCAt                 >  1:663764/1‑238 (MQ=255)
                                                                                                                                                                                                                                       cACGCGCGGGAACCCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGCAGCTGTACCCGTTCGGGATGAGCGGCGGCATGTTGCCGCGCATGTTGATTGCGTTGGCGGTGCTGTGTGAGTCACCGTTTCCCATCGCCGATGAACCGACCACCGACCTTGCCGTTGTAgtctcggcccccatcc               >  2:566885/1‑223 (MQ=255)
                                                                                                                                                                                                                                       cACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGCTGGCGGTGCTGTGTGAATCACCGTTTATCATCGCAGATGAACCGACCACCGGCCTTGACGTGGTAGCACCGGCTCGCAGcc               >  2:357007/1‑238 (MQ=255)
                                                                                                                                                                                                                                         cgcgcgGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCg            >  1:422992/1‑239 (MQ=255)
                                                                                                                                                                                                                                             ggggaacaCCTGCCTGGCGTTGGGGAACCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATc         <  1:498429/232‑1 (MQ=255)
                                                                                                                                                                                                                                                    aCCTGCGGGGCGTGAGGGACCCCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGa  <  1:1096417/239‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                
CCTGCAACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCAACGCTGGGCATTTTGCCCGCTGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCGCCTTGCGCCCTGCGCGGCATCAAAATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCACTGCACACCATGCACACCCACGCGCGGGAAACCTGCCTGGCGTTAGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCGCGCGTGCTGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCGTTTATCATCGCCGATGAACCGACCACCGACCTTGACGTGGTAGCACAGGCGCGCATCCTCGATCTGCTGGA  >  NC_000913/3617088‑3617564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: