BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,665,701	C→A	100%	A37S (GCG→TCG)	gadX ←	acid resistance regulon transcriptional activator, autoactivator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,701	0	C	A	49.9%	123.8 / 221.4	128	A37S (GCG→TCG)	gadX	acid resistance regulon transcriptional activator, autoactivator
Reads supporting (aligned to +/- strand): ref base C (53/9); new base A (1/62); total (54/74)
Fisher's exact test for biased strand distribution p-value = 1.36e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-16
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 128 total aligned reads displayed.

TCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGAT  >  NC_000913/3665465‑3665917

tctTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCa                                                                                                                                                                                                                           <  1:97789/238‑1 (MQ=255)

  ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑TATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCaata                                                                                                                                                                                                                        >  2:253806/1‑237 (MQ=255)

  ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCaaaa                                                                                                                                                                                                                        >  2:529742/1‑239 (MQ=255)

  ttttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCaaaa                                                                                                                                                                                                                        >  1:1220592/1‑239 (MQ=255)

    ttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCaaaaa                                                                                                                                                                                                                       >  2:818498/1‑238 (MQ=255)

          aGCATTTCTGCATAGCGGAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGCAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGACAC‑TTTTTT‑ATGTGCATGTAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGAATCCGCAAAAACC‑A‑GGt                                                                                                                                                                                                               >  1:736662/1‑238 (MQ=255)

          aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTc                                                                                                                                                                                                              >  2:855653/1‑239 (MQ=255)

          aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTc                                                                                                                                                                                                              >  1:640439/1‑239 (MQ=255)

          aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTc                                                                                                                                                                                                              >  2:753591/1‑239 (MQ=255)

            cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCa                                                                                                                                                                                                             >  1:61589/1‑238 (MQ=255)

            cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCa                                                                                                                                                                                                             >  1:177852/1‑238 (MQ=255)

            cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCa                                                                                                                                                                                                             >  1:287511/1‑238 (MQ=255)

              tttCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑AAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCAcc                                                                                                                                                                                                           >  2:596471/1‑238 (MQ=255)

                tCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGAAAAAACC‑A‑GGTCACCGcc                                                                                                                                                                                                        >  1:232399/1‑239 (MQ=255)

                    cATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTa                                                                                                                                                                                                    >  1:682208/1‑239 (MQ=255)

                      tAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTaaa                                                                                                                                                                                                  >  2:655353/1‑239 (MQ=255)

                         cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAAc                                                                                                                                                                                                                      >  1:346165/1‑218 (MQ=255)

                         cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATa                                                                                                                                                                                               >  2:873661/1‑239 (MQ=255)

                         cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATa                                                                                                                                                                                               >  1:190135/1‑239 (MQ=255)

                           aaGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGc                                                                                                                                                                                             >  1:350961/1‑239 (MQ=255)

                            aaatctttATTCGGCAATTGTGGCGAAACTTCCTAAAAAAC‑AGGAATACTCCTACTAAATGGATGACCGATAAATA‑AAAAAAT‑TTTTTT‑AGGTGCATGAAGTTTT‑AATGCCCCCT‑CCTTGAGCATCGGGAGAAATAATGAAG‑GCGTGTCCC‑TTAACACA‑AATAAAAAATTTC‑CAACCCACTTATCTACTAGAT‑TTTGG‑CTGGCATCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCg                                                                                                                                                                                            <  1:870919/233‑1 (MQ=255)

                              aTTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGat                                                                                                                                                                                          >  2:344323/1‑239 (MQ=255)

                                 tttATTCTGCGATAGTGGCACAA‑TTTCTAAAAAACAAGAAATATCTCTAAAAAAGTGATAAACGAGTAATA‑AAAAATT‑TTTTTT‑ATGTGCAAGAAGTTTT‑AATCCCTCCC‑CTTTGAGCATCGGAAAAAAAAATGAAG‑GCGTCTCCT‑TTGACACA‑AATAAAAAATTTC‑CAACACACTTTTCTCCTCGAT‑TTTGG‑CTTCCACCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                       <  2:1075989/238‑1 (MQ=255)

                                 tttATGCTGCGATGGTTGCACAACTTCCTAAAAAAC‑AGGAATATCTCTACTACAGTGAAGAACGAGTAATG‑AAAAAAT‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCAGGGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATAAAAAATTTT‑CAACCCACTTATCTCCTCGAA‑TTTGG‑CTTGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                       <  1:243369/238‑1 (MQ=255)

                                 cTTATTCAGCGATAGTTGCGCAACTTCCTAAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAATT‑TTTTTT‑ATGTGAATAAAGATTT‑AATGCCTCCT‑CCTGGAGCATCGGGAAAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTA‑CAACACATTTATCTACTCGAA‑TTTGG‑CTTGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATAtt                                                                                                                                                                                       <  1:41701/239‑1 (MQ=255)

                                        tGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                >  2:1157458/1‑239 (MQ=255)

                                        tGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                                                                                                >  2:1019604/1‑239 (MQ=255)

                                        tGCGATAGTTGCGCAACTTCCTAAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAAAAAC‑TTTTTT‑ATGTGCATGAAGTTTT‑AATCCCTCCT‑CCTTGAGCAGCGGGAAAAATAATAAAA‑GCGTGTCCC‑TGGACACA‑AATAAAAAATTTT‑CAACACCTTTATCTCCTAGAA‑TTTGG‑TTTGCACCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCAt                                                                                                                                                                                 <  1:78611/238‑1 (MQ=255)

                                          cGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTaaaa                                                                                                                                                                                                 >  1:488129/1‑220 (MQ=255)

                                          cGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                               >  2:403168/1‑238 (MQ=255)

                                          cGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTa                                                                                                                                                                               >  1:76088/1‑238 (MQ=255)

                                            atgtttGCACAACTTAATAAAAAAC‑AGGAATATCTTAACAAAAGTGAAGAACGATTAATG‑AAAAAAT‑TTTTTT‑ATGTACAAAAATATTT‑AATCCCCCCT‑CCTTGAACATCGGAAAAAAAAAGAAAA‑GCGTCTCCC‑TTGACACA‑AAAAAAAAATTTA‑CAACACTCTTTTCTACAAAAT‑TTGGG‑TTTCCACCCAAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                                                                                            <  2:350961/235‑1 (MQ=255)

                                            atggttcagcAACTCCCTAAAAAAC‑AGGAATATCTCTGCTACAGTGATGAAGAAGTAATG‑AAGAAAC‑TTTTTT‑ATGAGCATGAAGATTT‑AATCCCTCCT‑CCTTGAAAATGGGGAAAAAAAAGAAAG‑GCTTGCCCT‑TGGACACA‑AAAAAAAAATTTA‑AAACACACTTATCTTCTCAAA‑TTTGC‑CTTGCA‑CCAAAAAAACCGA‑GGTAACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                                                                                            <  2:76088/231‑1 (MQ=255)

                                             tAGTCGCGCAACTTCCAGAAAAAC‑AGGAATATCTCTACGACAATGATGAACGATAAATA‑AAGAAGT‑TTTTTT‑ATGCGCATGAAGATTT‑ATTCCCTCCT‑CTTTGAGCATCGGGAAAAATAATGAAG‑GCTTGTCCC‑TGAAAACG‑AATAAAAAATTAT‑CAACCCACTTATCTACATAAT‑TTTGC‑TTTGCACCCGCAAAAACC‑G‑GGAACCCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                                                                                                            <  2:657967/238‑1 (MQ=255)

                                               tttGAGCAACTGCATGAAAAAA‑AGAAATATCTCAAATACAGTAATGAACGATAAATG‑AAAAAAT‑TTTTTT‑ATGTGCATAAATATTT‑AATCCCCCCC‑CCTAGAGCATGGGGAAAAAAAATAAAG‑GCGTGCCCC‑TTGACAAA‑AAAAAAAAATTTT‑CACCACCCTTATCTCCCAAAT‑TTTGG‑CTTCCACCCAAAAAAACC‑G‑GGCCACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                                                                                                          <  2:876402/237‑1 (MQ=255)

                                                ttGCGCAACTTCCTGAACACC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                                                                                                        >  1:1183310/1‑239 (MQ=255)

                                                 tgcacaattaccaaaaaaaC‑ATAAATATCTCTACTATAGTGATGAACGAGTAATG‑AAAAAAC‑TTTTTT‑ATGGACATGAAGATTT‑AATCCCCCCT‑CTTTGACAATGGGGAGAAAAAATGAAG‑GCGTGTCCC‑TGGACACG‑AAAAAAAAATTTA‑CAACCAACTTATCTACCAGAA‑TTTGG‑TTTGCATCCACAAAAACA‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                       <  2:640439/225‑1 (MQ=255)

                                                 tGCGCAAATTCCAAAAAAAC‑AAGAATATCTCTACTACAGAGATGAATGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAAAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTGCTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GATCTCCACCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                       <  1:231471/239‑1 (MQ=255)

                                                  gcgcAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                                                                                                                                                                       >  2:78611/1‑238 (MQ=255)

                                                    gcaactctcaaaaaaaa‑AGAAATATCTTTAATAAAGTGATGAACGAGTAATA‑AAAAATT‑TTTTTT‑ATGGGAATGAAGATTT‑AACCCCCCCT‑CTTGGAGCATCGGAAAAAAAAATGAAA‑GCGTCTCCC‑TTGACACA‑AAAAAAAAATTTC‑CAACCCCTTTATCTACACAAT‑TTTGG‑TTTCCACCCAAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTga                                                                                                                                                                     <  2:181295/227‑1 (MQ=255)

                                                      aatctcataaaaaaa‑AGAAAGATCTATAAGAAAGGGATGAAAAAGTAATG‑AAGAACC‑TTTTTA‑AGGTGAATGAATATTT‑‑ATGGCTCCTGCCTGGAGAAAGGGGAAAAATAATGAAA‑GCGACGCCC‑TTAACAAG‑AAAAAAAAATTTA‑CAAAACACTTACCACCAGGAA‑GTGGG‑CTGGCACCCG‑AAAAACA‑AGGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGaa                                                                                                                                                                  <  2:1183310/230‑1 (MQ=255)

                                                          tCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                                                                                              >  1:1152614/1‑239 (MQ=255)

                                                             taaaaaaC‑AGAAATATCTCTACTAGAGGTAGAAACGAGAAAAG‑AAAAAAC‑TTTTTT‑ATGTGAATGAAGTTTT‑AATGCCTCCC‑CCTTAAGAAGCGGAAAAAAAAATGAAA‑GCGTGCCCC‑TGGCAACA‑AAAAAAAAATTTC‑CAACACCCTTATCTACAAAAT‑TTGGG‑CTTACATCCGAAAAAACG‑G‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                                                                                                           <  2:1006326/237‑1 (MQ=255)

                                                                 aaaC‑AGGAATATCTTTTCCACAGTAATAAAGGAGTAAGA‑AAGAAAC‑TTTTTT‑ATGTTGTTGAAAATTT‑AACCCCTCTT‑CCTTGAGCATGGGGAAAAAAAATGAAA‑GCGTGTCCT‑TTTACACG‑AAAACAAAATTTT‑CAACACACTTACCTACTCAAA‑TTGGG‑CTTGCACCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                                                                                                       <  1:930727/239‑1 (MQ=255)

                                                                          ttatCTCTAGTACAGTGATGACCG‑GTAAAGAAAAAAAC‑TTTTTT‑AGGTGAATAAATATTT‑AACCCCTCCG‑CTTAGGGAATGGAGAAAAATAATAAAA‑CCGTGCCCC‑TTGACAC‑‑AAAACAAAATTTTCCAACACACTTATCTACTCAAA‑TTTGC‑CTTGCATCCGAAAAACCA‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  1:908627/238‑1 (MQ=255)

                                                                          atatCTCTACTACAGTTAAGAACGAGAAATG‑AAGAAAT‑TTTTTT‑AGGTGCATGAAGATTT‑AAGGCCTCCT‑CCTTGAGCAGCGGAAAAAAAAATAAAA‑GCGTGTCCC‑TTGACAGG‑AATACAAAATTTT‑CAACACACTTATCTACTCAAT‑TTTGG‑CTTGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  1:529742/239‑1 (MQ=255)

                                                                          atatCTCTACTACAGTAATAAACGAGTAAGG‑AAGAACT‑TTTTGT‑ATGTGTATAAAAATTT‑AATGCCTCCC‑CTGTGACCATGGGGAAAAAAAATGAAG‑GCGTGTCCT‑TTGACACG‑AATACAAAATTTA‑CAACACCCTTATCTACTGGAT‑TTTGG‑CTTGCACCCGAAAAAACC‑A‑GGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  1:753591/239‑1 (MQ=255)

                                                                          atatCTCTAATAAGGGAATGAACGAGTAAAG‑AAGAAGT‑TTTTTT‑AGTGGAAAAAATATTT‑ACTGACCCCC‑CCTTGGGCAGGGGAAAAAATAATGAAG‑GCGGGTCCC‑TTGAAACA‑AAAAAAAATTTTA‑AAACACACTTACTTACTC‑AA‑TTTGGTCTTCCATCCAAAAAAACG‑G‑TTCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  2:1208848/239‑1 (MQ=255)

                                                                          atagCTATACTACAGTGAAACACGTGAAATG‑AAAAATA‑TTTGTT‑ATGTAGATAAAGATTA‑AACGCGCCCG‑CCTTTGGAATGGGGAGAAAAAATAAAA‑GTGGTCCCC‑TGGACTGA‑AAAACAAAATTTC‑CAACCCACTTATC‑AATCAAATTTTGG‑TTTACACCCCAAAAACCC‑A‑TGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  2:472084/239‑1 (MQ=255)

                                                                          atactcctcctgcAGTGATGAACGAGTAATG‑AA‑AAAC‑TTTTTTGATGTGAATGAAGTTTT‑AATGCCTCCT‑CCTGGAGCATCGGGAAAAATAATGAAA‑GCGTGTCCC‑TGAACACA‑AATACAAAATTTT‑CAACACACTTATCTCCTCAAT‑TTTGG‑CTTGCATCCGCAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  1:398437/227‑1 (MQ=255)

                                                                          atacctttataaagttgatgaACGATTAAAG‑AAGAAAC‑TTTTTT‑ATGTGAAGAAATATTT‑AATGCCTCCT‑CCTTGACAATCGGAAAAAATAATAAAA‑GCGGGTCCC‑TTGACACA‑AATACAAAATTTT‑CAACCCATTTATCTACACAAA‑TTTGG‑ATTACAACCAAAAAAACG‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  2:71335/224‑1 (MQ=255)

                                                                          atacatctaaaacAGTGAAAAACGAGAAAAA‑AAAAAAT‑TTTTTT‑ATGTGCAAGAATATTT‑AACGCCCCCC‑CCTTGAGCAGCGGGAAAAAAAATGAAA‑GCGTGCCCC‑TTAACACA‑AATAAAAAATTTT‑AAACACACTTATCTACACGAA‑TTTGG‑TTTGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                                                                                               <  2:682208/228‑1 (MQ=255)

                                                                                   ctacAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCAtt                                                                                                                                                                                                     >  1:522291/1‑176 (MQ=255)

                                                                                     tacggaaagaaagtaaaaag‑aaaaaACTTTTTTT‑ATGAGTAAGAAAATTT‑AATGCCCCCT‑CCAGGAGAACGGGAAAAAATAATAAAG‑GCGTGCCCC‑TGGACAAA‑AAAAAAAAATTTC‑CAACCCCTTTATCACCGAAAT‑TTTGT‑TTTGCACCCGAAAAAACC‑G‑GTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGa                                                                                                                                     <  2:127976/220‑1 (MQ=255)

                                                                                     aaaGTGATGAGCGAGTAATA‑AAGAATT‑TTTTTT‑ATGTGAATGAATATTT‑AATCCCTCCT‑CCTGGAGCATGGAGAAAAATAAGGAAG‑GGTTGCCCT‑TTGA‑ACGAAAAAAAAAATTAC‑CAAACCCCTTTTCTCCACGAA‑TTTGG‑TTTGCACCCAAAAAACCA‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                                                                                                    <  2:412950/237‑1 (MQ=255)

                                                                                       atgtataaaCGAGTAATA‑AAGAACT‑TTTTTT‑ATGTGCATAAATATTT‑AATGCCTCCT‑CCTTAAGAAGAGGGAAAAATAATAAAG‑GTGTGTCCT‑TTGACAAG‑AATAAAAAATTTT‑CAACCCACTTATCTACTCAAT‑TTTGG‑CTTGCACCCGAAAAACCC‑A‑GTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                  <  1:559710/232‑1 (MQ=255)

                                                                                       aGTGATAAACGATTAATA‑AAGAATT‑TTTTTT‑AGGTGAATGAATATTT‑AATGCCTCCC‑CCTTGAGCAGCGGGAAAAAAAATGAAG‑GCGTGTCCC‑TTAACACG‑AATAAAAAATTTT‑CAACACACTTATCTACTAAAT‑TTTGG‑CTTGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                  <  1:818498/239‑1 (MQ=255)

                                                                                       aGTAATAAACTAGTAATG‑AAAAAAC‑TTTTTG‑ATGTGAATAAAATTTT‑ATCGCCCCCC‑CTGTTATAAGCGGAAAAAATAATAAAA‑TGGTTCCCC‑TTAACACA‑AAAACAAAATTTC‑CAACACCATTATCTCTTCAAT‑TTTGG‑CTTGCCCCCGAAAAAACC‑A‑GGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                  <  2:684181/239‑1 (MQ=255)

                                                                                        aggaatgaaggataaagaaagaaAT‑TTTTTT‑ATGTGCAAGAATATTT‑AATCCCTCCT‑CCTTGAGCAGGGGAAAAAAAAATGAAA‑GGGTTTCCC‑TTGACCCA‑AATAAAAAATTTC‑CAACACATTTATCTACTCAAA‑TTGGG‑CTGGCAACCGAAAAAACG‑A‑GTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                                                                                                  <  1:1029667/221‑1 (MQ=255)

                                                                                         tgatgaACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatatat                                                                                                                                                             >  1:19961/1‑210 (MQ=255)

                                                                                         tgatgaACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                 >  1:412950/1‑238 (MQ=255)

                                                                                         tgatgaACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAt                                                                                                                                 >  1:453972/1‑238 (MQ=255)

                                                                                          gagaaaagAGTAATG‑AAGAAAT‑TTTTTT‑ATGTGCATGAAGATTT‑AATCCCTCCT‑CCTTGAGCATCGGGAAAAAAAAGGAAA‑GCGTTTCCC‑TTGACACG‑AATAAAAAATTTT‑CAACCCTCTTATCTACTCGAA‑TTTGG‑TTTGCACCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAAtt                                                                                                                                <  1:740875/231‑1 (MQ=255)

                                                                                           atgaACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTc                                                                                                                               >  1:287978/1‑238 (MQ=255)

                                                                                            aaaaCGAGTAAGA‑AAAAATT‑TTTTTT‑AGGTGCATAAAGATTT‑AATGCCCCCT‑CCTGGAGCATGGGGAAAAATAATAAAA‑GCGTGCCCC‑TTAAAAAA‑AAAAAAAAATTTA‑CAACACTTTTATCTACTCGAA‑TTTGG‑CTTGCACCCAAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                                                                                             <  1:1157458/237‑1 (MQ=255)

                                                                                             taaaggagaaagaaaaaaaC‑TTTTTT‑TTGTACAAGAAAATTT‑AATGCCCCCT‑CCTAGAGCATCGGGAAAAAAAAGAAAG‑GCGTGTCCC‑TGGACAAG‑AAAAAAAAATTTT‑AAACCCCCTTATCTACTGAAA‑TTTGG‑CTTGACCCCGCAAAAACA‑G‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTccc                                                                                                                             <  1:655353/223‑1 (MQ=255)

                                                                                                cgataaaTG‑AAGAAAT‑TTTTTT‑ATGTGCATGAAGATTT‑AATCCCCCCT‑CCTTGAGCATGGGGAAAAATAATAAAA‑GCGTGTCCC‑TTGACACA‑AATACAAAATTTT‑CAACACACTTATCTACTCAAT‑TTTGG‑CTTGCACCCAAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                         <  1:170393/234‑1 (MQ=255)

                                                                                                  ataaagg‑aaaaaTC‑TTTTTT‑ATGAGGATAAATATTT‑AGTGCCTCCT‑CCTGTAGCATCGGAAAAAATAATGAAA‑GCGTCCCCT‑TTAAAAAA‑AATAAAAATTTTT‑CAACCCTCTTATCTACTCAAT‑TTTGG‑CTTGCATCCAAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                                                                                                         <  2:460463/231‑1 (MQ=255)

                                                                                                   gaaaTA‑AAGAAAG‑TTTTTT‑ATTGGCAGGAAGTTTT‑AATGCCTCCT‑CGTGAAACATCGGAAAAAAAAATAAAAGGCGT‑CCCC‑TTAACAAG‑AATACAAAATTTT‑CAACCCACTTATCTACACTAT‑TTTGG‑TTTCCCTCCGAAAAACCC‑A‑GATCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                                                                                                       <  2:363214/236‑1 (MQ=255)

                                                                                                     aaTG‑AAAAACT‑TTTTTT‑ATGTGAATAAAGATTT‑AATGCCCCCT‑CCTTGAGCATCGGGAAAAATAATGAAA‑GCGTGTCCC‑TTGCAACA‑AATAAAAAATTTA‑CAACACCCTTATCTACTCAAT‑TTTGT‑CTTGCATCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                                                                                    <  2:1220592/239‑1 (MQ=255)

                                                                                                     aaTA‑AAAAAAT‑TTTTTT‑ATGAGAAAGATGATTT‑AACGCCTCCC‑CGTTGAGCTAGGGGAAAAAAAAAGAAA‑GCGTGTCCC‑TTGACAGA‑AATACAAAATTTA‑AAACACTTTTATCTACTCGAT‑TTTGG‑TTTCCGCCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                                                                                    <  1:253806/239‑1 (MQ=255)

                                                                                                     aaTA‑AAAAAAT‑TTTTTT‑ATG‑GCAATAAAATTTAAATCCCCCCT‑CTTGGAGAATGGGAAAAAAAAATGAAG‑GCGGGCCCC‑TTAACACA‑AATAAAAAATTTT‑CACCACCTTTATCTACTCGAT‑TTTGG‑CTTGCACCCGAAAAAACA‑A‑GGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGa                                                                                                                    <  1:873661/239‑1 (MQ=255)

                                                                                                       tG‑AAGAAAT‑TTTTTT‑ATGTGCATGAAAATTT‑AATTCCTCCT‑CTTTAAACAAGGGGAAAAATAATGAAA‑GTGTTTCAC‑TTGACAAA‑AATAAAAAATTTT‑CAACACACTTATCTACTCAAA‑TTTGG‑TTTCCACCCAAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAtt                                                                                                                  <  2:527989/239‑1 (MQ=255)

                                                                                                       tG‑AAGAAAC‑TTTTTT‑ATGCGCATGAAGATTT‑AACCCCCCCC‑CTTTGAGCATGGGGAAAAAAAAAAAAA‑GCGTGTCCC‑TTGACACA‑AAAAAAAAATTTT‑CAACACCTTTATCTACTCAAT‑TTTGG‑CTTGCACCCAAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAtt                                                                                                                  <  1:906208/239‑1 (MQ=255)

                                                                                                        g‑aagaaAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGAtt                                                                                                                  >  1:460463/1‑238 (MQ=255)

                                                                                                          aagaaAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTAt                                                                                                                                                         >  1:830301/1‑198 (MQ=255)

                                                                                                               tc‑TTTTTT‑ATGTGCATGAATTTTT‑AATCCCTCCT‑CTGTGAGCATGGGGAAAAATAATAAAA‑GGGGTTCCC‑TGAACAAG‑AATAAAAATTTTT‑CAACACTCTTATCTACTCGAT‑TTTGG‑CTGGCACCCAAAAAAACC‑G‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                           <  1:403168/238‑1 (MQ=255)

                                                                                                               tc‑TTTTTT‑ATGAAGATGAAGTTTT‑AACGCCCCCC‑CCTAGAACATGGGAAAAAATAAAAAAA‑GCGTTCCCC‑TTGACCAA‑AAAAAAAAATTTA‑CAACCCACTTATCTATAAAAT‑TTTGG‑CTTCCACCAGAAAAAACG‑G‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                           <  2:42587/238‑1 (MQ=255)

                                                                                                                cttttttt‑AGGGGAAGGAATATTT‑AATGCCCCCT‑CTTTGAGCATGGGAAAAAATAATAAAG‑GCGTGTCCC‑TTGAAACA‑AAAACAAAATTTC‑CACACCATTTATCTCCCAAAT‑TTTGG‑CTTCCACCCAAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                           <  1:1019604/237‑1 (MQ=255)

                                                                                                                attttttt‑AGGTGCATGAATATTT‑AATCCCTCCT‑CCTTGAGCATCGGGAAAAATAATAAAG‑GCGTGTCCC‑TTGACACA‑AATAAAAAATTTT‑CAACACACTTATCTACTAGAA‑TTTGG‑CTTGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                           <  1:850570/237‑1 (MQ=255)

                                                                                                                  tttttt‑ATGTGCTAAAATATTT‑AATGCCTCCT‑CCTTGAGCATAGAGAGAAATAATGAGC‑AC‑TGCCCCTTTGAACAA‑AAAAAAAAATTTA‑AAACACTCTTATCCTCTAGAA‑TTTGG‑CTTGAATCCGAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                                                                                                           <  1:1115466/237‑1 (MQ=255)

                                                                                                                     ttt‑ATTTGCATGAAGATTT‑AATGCCCCCT‑ACTTGATCACCGGAAAAAATAAGAAAA‑GCGTGCCCC‑TTGACCCG‑AATAAAAAATTTA‑CAACACACTTATCTCCTCGAT‑TTTGG‑CTTGCACCCGAAAAACCT‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCCTAGTTGACt                                                                                                      <  1:734428/239‑1 (MQ=255)

                                                                                                                     ttt‑ATGTGAATAAATATTT‑ACTCCCCCCT‑CATGAAGAATGGGAAAAAAAAATGAAG‑GCGTTTCCT‑TTAACACA‑AATAAAAAATTTT‑CAACCCATTTATCTCCTAAAT‑TTTGT‑TTTCCACCAGAAAAAACG‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                      <  2:453972/239‑1 (MQ=255)

                                                                                                                     ttt‑ATGGGCATGAAGATTT‑AATGCCCCCT‑CCTTGACCATCGGGAAAAATAATGAAA‑GCTTTTCCT‑TTGACACA‑AATAAAAAATTTT‑CAACACACTTATCTACTCAAA‑TTTGG‑TTTGCACCCGAAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                      <  2:177852/239‑1 (MQ=255)

                                                                                                                      tttattataataaaGATTT‑AATCCCCCCT‑CCTTGAGCATCGGGAGAAAAAATGAAA‑GCGTGTCCC‑TTGACAAG‑AATAAAAAATTTT‑AAACACACTTACTTCCTGGAA‑TTTGG‑CTTGCATCCGAAAAAACC‑A‑GTTCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                                                                                                      <  1:344323/227‑1 (MQ=255)

                                                                                                                             gaagaaaGATTT‑AATGCATCTC‑GCTTGAGCAGCGGAAAAAAAAATGAAG‑TGTTCTCCC‑TAGAAACA‑AAAAAAAAATTTC‑CAACAGTCTTATCTAGAAAAT‑GTTGG‑TTTGCAGCATCAAACACC‑A‑GCTAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                                                                                                <  2:333533/233‑1 (MQ=255)

                                                                                                                                 gAAGTTTT‑AACGCTTCCG‑CTTTGAGCATCTGCAGAAAAAATGAAT‑GCGTGTCAC‑TTGACACA‑AAAAAAAAATTTT‑CACCACATTTATCTACTACAA‑TTGCG‑TTTGCAGCCACAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATTGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                           <  2:549189/239‑1 (MQ=255)

                                                                                                                                   ataTTT‑AATGCCCACG‑CCGAGAGCATGGTGAAAAATAATGAAG‑GCGGCTCCC‑TTCAACAG‑AATAAAAAATTTA‑CAACCCACTTATCTACTCTAC‑TTTGT‑CTCGCAT‑CGTAAAAACCGG‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATaa                                                                                         <  2:1250082/237‑1 (MQ=255)

                                                                                                                                   aGATTT‑AATGCCCCCC‑CCTTGACAATGGGGAAAAATAATAAAA‑GCGTGTCCT‑TTGACACA‑AAAAAAAAATTTT‑AAACACACTTTCCAACACGAA‑TTTGG‑TTTGCACCCAAAAAAACG‑G‑GGAAACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                           <  2:287978/237‑1 (MQ=255)

                                                                                                                                   aGATTT‑AATCCCTCCT‑CCTTAAGAATGGGAAAAAATAAGAAAA‑GCTTGCCCC‑TTGACACA‑AATAAAAAATTTC‑CACCACACTTATCTCCTAGAT‑TTTGT‑CTTCCACCCAAAAAAACA‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt                                                                                           <  2:120643/237‑1 (MQ=255)

                                                                                                                                                 cct‑cttTGAACATCGGGAAAAATATGAAAA‑GCTTGTCCC‑TTGACAAA‑AATACAAAATTTA‑CAACACACTTATCTACTCGAT‑TTTGG‑CTTGCACCCAAAAAACCC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGtt                                                                            <  1:1205789/239‑1 (MQ=255)

                                                                                                                                                  ct‑cctTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACa                                                                                            >  1:591489/1‑222 (MQ=255)

                                                                                                                                                            cATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTg                                                                                                                 >  1:27756/1‑192 (MQ=255)

                                                                                                                                                               cGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACa                                                                                      >  1:1044842/1‑216 (MQ=255)

                                                                                                                                                                                             gACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTaa                                                                      >  1:803911/1‑204 (MQ=255)

                                                                                                                                                                                             gACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAAttgattga                                   >  1:786471/1‑239 (MQ=255)

                                                                                                                                                                                             gACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAAttgattga                                   >  2:259445/1‑239 (MQ=255)

                                                                                                                                                                                                                    acacacTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCg              >  1:104393/1‑239 (MQ=255)

                                                                                                                                                                                                                                aCTCGAA‑TTTGG‑CTTGCACCCGAAAAACCC‑A‑GGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGgat  <  1:855653/239‑1 (MQ=255)

                                                                                                                                                                                                                                  tCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc                                                                                       >  1:1140905/1‑152 (MQ=255)

TCTTTTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAAC‑AGGAATATCTCTACTACAGTGATGAACGAGTAATG‑AAGAAAC‑TTTTTT‑ATGTGCATGAAGATTT‑AATGCCTCCT‑CCTTGAGCATCGGGAGAAATAATGAAA‑GCGTGTCCC‑TTGACACG‑AATACAAAATTTT‑CAACACACTTATCTACTCGAA‑TTTGG‑CTTGCATCCGCAAAAACC‑A‑GGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTACTGTTTATTAATGTAGCACGCCGCCCTCTTAAGTCAAATTGATTGATAAATAAATTTAAACGAAGCGGGAGAACAGGAT  >  NC_000913/3665465‑3665917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`