| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,782,907 | T→G | 27.1% | T252P (ACC→CCC) | gpsA ← | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,782,907 | 0 | T | G | 27.1% | 84.8 / 8.9 | 74 | T252P (ACC→CCC) | gpsA | glycerol‑3‑phosphate dehydrogenase (NAD+) |
| Reads supporting (aligned to +/- strand): ref base T (21/28); new base G (19/2); total (43/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCC > NC_000913/3782669‑3783141 | gCACGACCTAGTAAAGTCAATTCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGt < 2:23438/239‑1 (MQ=255)gCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGt < 1:983328/239‑1 (MQ=255)gCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGt < 1:387423/239‑1 (MQ=255) aGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTTCGCGCACGGTTGTCGGGACAGGTGAGCAccca > 2:387423/1‑237 (MQ=255) aGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAg < 2:667302/239‑1 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTTACCGACTGGGTGTCGGTGCAGGGAAGCACCaaaaagcca > 2:530998/1‑231 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGCCTGGTTGTTGGTACAGGGAAGCACCAAATCCCCa > 1:433923/1‑239 (MQ=255) tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGCTAAGCGACCGGGTGTTGGGACAGGTAAGCACCAAAACGCCa > 2:1206744/1‑239 (MQ=255) tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTTGGGACCGGCAAGCACCAGAACGCCaaac > 1:82946/1‑236 (MQ=255) tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGcc < 1:30353/239‑1 (MQ=255) ccTCGCGCGCTTTTTTTCCGAAATATAATTCTTGATAAATTTCCTCGTTTATTGGCTTTTCACCGCCG‑AAGCGATGCGCCATTTCGCGTACTTCTTTCGTATTGCGGTAGCCTTCCCCCACCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGcc < 2:966700/239‑1 (MQ=255) ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTTCGCGACTGGTTGTCCGTACAGGGAAGAACCAGAACGCCCAAcccccc > 1:501866/1‑236 (MQ=255) ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTGTCACGCCTCGCTGTCGGCTCACGGCAGCACCTAACAGCCAAGcccccc > 2:273988/1‑236 (MQ=255) ccgggcgcgTTTTTTCCGCAATATAATACTTGAGAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCAt < 1:767203/235‑1 (MQ=255) cgcgcgTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCCGAACGCCAAGCCCCGCCATga > 1:97778/1‑237 (MQ=255) ttGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCTACTGGTTGTTGGTACAGGTAAGCCCCAAAACGCCCAGCCCCGCCCTGCCCATAAAGGTGGCACGGTCGGCAcca > 1:557134/1‑237 (MQ=255) ttGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCCGCTGGTTGTCGGTACAGGGAAGAACCAGATCGCCCAGCCCCGCCATGCCCCTAAAGGTGGCAGGGGTGGCAccc > 1:985111/1‑238 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTTCGCGGCTGGTTGTCGGGAAAGGGAAGGACCAAATCGCCAAACCCCGCCATGCCCACAAAGGGGGGAGGGTCGGCCCCCCgc > 1:186201/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGCCTGGTTGTCGGGACAGGGAAGAACCAGATCGCCAAGCCCCGCCCTGCCCATAAAAGTGGCAGGGTGGGCACCCCgc > 1:1161013/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCACAACGCCCAGCCCCCCCATGCCCCCAAAGGTGGCAGGGTCGGGACCCac > 2:637378/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAGATCCCCCAGCCCCCCCCTGCCCATAAAGGGGGCCGGGGCGGGCCCCAgc > 1:882197/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAACACCAAAACCCCAAGCCCCCCCCTGCCCATAAAAGAGGGAGGGGCGGGACCCAg > 2:682614/1‑238 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTAAAGGTAAGCACCAAAACGCCAAACCCCGCCATGCCCCTAAAAGTGGCCGGGGCGGCACCCac > 1:743100/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACCGGTTGTCGGTACAAGTAAGCCCCAAATCCCCCAGCCCCCCCCCGCCCCTAAAAGTGGCAGGGGCGGCccccct > 1:281495/1‑236 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCCACTGGTTGTCGGTTCAGGTAAGGACCAAATCGCCAAACCCCCCCCTGCCCCTAAAGGGGGCAGGGGCGGCACCCAg > 2:732277/1‑238 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACCCGACTGGTTGTCGGTACAGGTAAGCACCAGAACGCCCAACCCCGCCATTCCCCTAAAGGGGGGAGGGTCGGCATCCac > 1:711061/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACCCCGCTGGTTGTCGGGAAAGGTCAACACCAAAACCGCCAGCCCCCCCCAGCCCCTAAAGGTGGGCGGGGCGGCACCCac > 1:526344/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACCCCACTGGTTGTGTGTAAAAGGCAGAGCCAGAAAGCTAACCCCCCCCCAGCCCCTAAACGTCGTATGGCAGGCACCCaa > 2:1125273/1‑237 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACACGACTTGTTTTCGGGACAGGGAAGCACCAAAACGCCAAACCCCCCCATGGCCCTAAAGGGGGCAGGGGCGGGACCCAgc > 1:427033/1‑239 (MQ=255) gATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTAACAGACTGGTTGTCGGTTAAGGGAAGCACCAGAACGCCAAACCCCCCCCAGCCCCTAAAGgggggcggcggcaccccgac > 2:244001/1‑222 (MQ=255) tAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCCGCTGGTTGTCGGTACAGGTAAACACCAGAACGCCCAACCCCGCCCTGCCCATAAAGGGGGCAGGGGCGGCACCCAccg > 1:588266/1‑238 (MQ=255) gaaaTTCCCGCGGTTAGT‑GCCTTTCAAACCCGAAAGTGAGGCCCCTGTTCGCTGACTTTTTTCTTGTTGCGGTCGCCCCCCCCCTCCTGCCCAGTCTGCTCCGGCGGGTTTTGTTC‑TCCCGTCCCTGGCCCAGGCCTCACGACAAACCGGCGTTTCCGCGCCGGGGTGCGGGTACAGGTAACCCCAAAATCACCACGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAgcgc < 2:248860/238‑1 (MQ=255) ttATTGGCATTTCACCGCCG‑AAGCGATGCGCCAGTCCGCGGACTTCTTTCGTATGGCGGTAGCCTTCCACCACCGGACCAATCTCCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCAcc < 2:214218/220‑1 (MQ=255) ttCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAGATCGCCAAACCCCCCCCCGCCCCTAAAGGTGGCAGGGGCGGGACCCAGcgccccccctggctcttttacacat > 1:214218/1‑217 (MQ=255) tCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcag‑c < 1:1103245/239‑1 (MQ=255) cG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGGCCGG‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCa < 1:1125273/239‑1 (MQ=255) cG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGCCCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCa < 1:530998/239‑1 (MQ=255) cgcCAGTTCGCGGACTTCTTTCGTATGGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCAGGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCa < 1:1224288/239‑1 (MQ=255) ttCGCGGACTTTTTTCTTATTGCGGTAGCCTCCCCCCCCCTCACCATTCTTCTCCTGCGCTCTTTGTCCATCCTTGCCCTGCCCGA‑GCATCATGCCAAACCGGCGGTTACGCGCCTGGTTGTGGGTACAGGTAAGCCCCAGAGCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCg < 2:108712/239‑1 (MQ=255) cgcgGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGCAAGCACCAAAACGCCAAACCCCCCCCCGCCCCTAAAGGGGGCAGGGGCGGGCCCCCACCCCGCCCCCAGACGCCGCATTTTCG‑CCAGCCCCCGGGGGGTCAGCCCCgg > 2:419411/1‑237 (MQ=255) tcttTCGTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTCCGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCAtt < 1:1206744/239‑1 (MQ=255) tcttTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCAtt < 1:732277/239‑1 (MQ=255) tcttTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTAACCGAACGCAGGGCCGGACAGGCGAGCACCAGAACGGCAATCCCCGCCCTGCCCCCAACGGGGGCCGGGTCGCCATCCACCCCCCCCCCCACACGCGGCACATCTG‑CCAGCCCACCGGCGATCCGCGCCGCACGCGGAt > 2:241186/1‑238 (MQ=255) tcttCCGTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCGCCGCGCTTTGTCCATCCATGCCCTGCCCGA‑CCATCATGCCAAAACGGCGGTTCCGCGACTGGTGGTCGGTACAGGTAAGCCCCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCAtt < 2:82946/239‑1 (MQ=255) gTATGGCGGTAGCCTCCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACACCCAGGCCCTGACCGA‑GCATCATGCAAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACAAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc < 2:1161013/239‑1 (MQ=255) tATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAACCGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAgcg < 1:735643/219‑1 (MQ=255) tATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCCACCGGGTTTCGGTACAGGTAAACACCCAATCGCCAAGCCCCGCCCTGCCCCTAAAGGGGGCAGGGGCGGCACCCACCGCCCCCCCCCCACGCCACATTTCCG‑CCCGCCCACGGGGGCTCAGCGCCGCACGCGCCTTCGcccc > 2:110445/1‑238 (MQ=255) ttGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTTACCGGCTTGGTGTCGGGACAGGGGAGCACCAAATCACCAAGCCCCCCCATGCCCCTAAAGGTGGCAGGGGCGGCCCCCCGCGCCCCCCCCACACGCCACATTTCAG‑CCCACCCCCCGGGGGTCCGCGCCCCACCCGCCTTCGCCCCcaa > 2:920192/1‑239 (MQ=255) cGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACCCGCCTGGTTGTCGGTACAGGTAAGCACCAAAACGCCCAGACCCCCCCCGCCCCTAAAAGGGGCAGGGGCGGGCCCCACCGCCGCCCCCAAACCCGGCCATTTAG‑CCCGCCCCCGCGGGCGCAGCGCCgccaccgccttcccccccaaac > 2:579834/1‑217 (MQ=255) gcgCCTTCCCCCCCCTGACCACTCTCCTACTGCGCGCTTTGGACATCCGTGCCCTGCCCGA‑GCATCATGCCAAAACGGCGGTTTCGCGATTGTTGGCCGGTACGGAGAAGCCCCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGTTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa < 2:172381/236‑1 (MQ=255) gCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGCCTGGGTGTCGGTAAAGGTAAGAACCAAAACGCCAAACCCCCCCCTGCCCATAAAGGTGGGCGGGGGGGCCCCCCACGCCGCCCCCAGAACCGGCACTTCCA‑TCAGCCCCCGGGGGATACGCGCCGGCCgcgccctgcctcttataaacacc > 2:735643/1‑219 (MQ=255) gCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTTGTTTTCGGTACAGGTAAGCACCACATCGCCCAGACCCCCCCAGCCCATTAAAGGGGCAGGGGGCGCCCCCCGCCCCGCCCCCAGCAGCGCCACTTCCGCCCAGCCC‑CGGGGGGTCAGCCCCGGACacgccactcccccccaacccaca > 2:688544/1‑219 (MQ=255) gCCTCCCACCCCCTGACCAATCTTCCCCTGCGCGCTGTGTACATCCATGCCCTGACCGA‑GCACCAGGCAAAACCGGCGGTTCCGCGACTGGTTGTCGTTCCAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATAc < 2:400869/239‑1 (MQ=255) ccccccTGCCCAACCTTCTCCTGCGCGCTTTGTACACCCATGCCCTGCCCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGa < 2:97778/235‑1 (MQ=255) ggACCAATCTTCCCCCGCGCGCTTGGTACACCCATCCCCGGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATccc < 2:588266/238‑1 (MQ=255) ccaaaTCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAACCGGCGGTTCCGCGACTGTTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCg < 1:682614/236‑1 (MQ=255) aCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTTCGCGCCTGGTTTGCGGTACAGGGAAGCACCCGACCGCCAAGCCCCCCCCTGCCCCTAAAGGGGGCAGGGTCGGCACCCCGCGCCGCCCCCAGCCCCGCCATTTCCT‑CCCGCCCCCGGGCGGTCCCCGCCGCACGCGCATTCGCCCCAACACCGACACTGCC‑GGACCTcccc > 2:466317/1‑238 (MQ=255) ttctCCTGCGCGCTTTGCACACCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAAt < 1:449295/239‑1 (MQ=255) ctccgcgtTTTGTACACCCATCCCCTAACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACGGGTTGTCGGTGCAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCaa < 2:957481/236‑1 (MQ=255) cgcgcTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTAAGCGACCGGTTGTCGGTACAGGGAAACACCAAAACGCCAAACCCCCCCCCGCCCCTAAAAGGGGGAGGGGCGGCACCCAGCGCCGCACCCAAACGCCACACTTCAG‑CCAGCCCCCCGGGGGTCAGCGCCGCGCGCGCATTCGCACCACAACCCACACCCTC‑CGGCAACCCCCCACCCACGGCAaaaa > 2:438514/1‑239 (MQ=255) acccttccccttcccGA‑GCACCATGCCAAAACGGCGTTTCCGCGACGGTTTGTCGGCACGGGTAAGCACCACTCCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg < 2:882197/226‑1 (MQ=255) tGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACTCGCCTTGTTGTCGGGACAAGGAAGCACCAAAACGCCAAGCCCCCCCCCGCCCATAAAGGTGGCAGGGGCGGCCCCCCCCGCCGCACCCACACGCGCCCTTTCAG‑ACAGCCCCCGGGGGAACCACGCCCCACTCCCCCTCTCACCAACACCGCCCCCCCC‑CGACATCCCCCCCCCCACCGCAATAACCGTTTTCACCCcgcc > 2:474131/1‑238 (MQ=255) gCCCTGACCGA‑GCATCATGCCAAACCGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCaa < 1:1186152/218‑1 (MQ=255) ccTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCA‑‑GTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAg < 1:244001/239‑1 (MQ=255) aCCGA‑GCATCATGCCAAAACGGCGGTTACGCGGCTGGGTGTCGGTACAGGTAAGGACCAGATCGCCAAGCCCCGCCCACCCCCTAAAAGGGGCAGGGGCGGGACCCCCCGCCGCACCAAAACGCGCCATTTCCG‑CCACCCCCCCGGGGGTAACAGCCGTCAGCGCCTTCGCACCCAAACCCATAACGCCGGGCCACCCCCCCCCCACCAGC‑ATAACGCTTTTCTCCCCgccgcccacc > 2:645411/1‑234 (MQ=255) cagggacagtGCCAAAACGGCAGGTAGGCGAGTGGGTGGCGGTACGGGCAAGCACCAGCCCGCAAACCCCCGCCATGCCCATAAAGGTGGCAGGGGCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg < 2:1066083/229‑1 (MQ=255) a‑GCATCATGCCAAAACGGCGGTTACGCGACCGGTTGTCGGTACAGGGAAACACCAAATCGCCCAGCCCCCCCCTGCCCCCAAAGGGGGCCGGGGCGGCACCCCGCCCCCCACCCAAACGCCACATTTTAG‑CCCGCCCCCCGGGGATCAGCGCCCTACCCGCCTTCCCCCCCAAACCCAAACCCGC‑GGGCATCCCCCCCCCCACGGCCAAAACGTTTTTcctttttctttttcacctctc > 2:1186152/1‑219 (MQ=255) aaacCGGCGGTTACGCGACTGGTTGCCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAAt < 1:637378/239‑1 (MQ=255) gcggTTCCGCGACGGGTTGTCGGTACAGGGAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa < 2:557134/238‑1 (MQ=255) gggTGACGCGACGGGTGGGCGGAACAGGTAACCCCCAGATCGCCAACCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGAt < 1:466317/237‑1 (MQ=255) cgtttACGCGACTGGTTGTCGGTAAAGGTAAGCACCAGACCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGAt < 1:115899/235‑1 (MQ=255) aCTGGGTGTCGGGACAGGGAAGCACCAGATCGCCAAGCCCCCCCATGCCCATAAAAGGGGGAGGGGCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑ACAGCCCCCGGGTGATCAGCGCCGTACGCGCACTCGCACCAAAACCCAAACCGTC‑GGACAACCCCCCCCCCATGGCAATAACGCTTTTCACCGCCCCGCCAAGCTGCACCCCCAGTAAATCCGGATTGCTGGAAAc > 2:1000458/1‑238 (MQ=255) cGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACtt > 1:692224/1‑239 (MQ=255) ggTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACttt > 1:573915/1‑239 (MQ=255) ggTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACttt > 1:663065/1‑239 (MQ=255) cAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc > 2:133958/1‑239 (MQ=255) aGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc > 2:937412/1‑238 (MQ=255) | GCACGACCTAGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCG‑AAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGA‑GCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAG‑CCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTC‑GGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCC > NC_000913/3782669‑3783141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |