Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,782,912 A→C 15.3% V250G (GTG→GGG)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,782,9120AC15.3% 129.4 / 2.5 77V250G (GTG→GGG) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base A (43/20);  new base C (2/11);  total (46/31)
Fisher's exact test for biased strand distribution p-value = 1.07e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782678‑3783149
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
aGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGCACGGTTGTCGGGACAGGTGAGCAccca                                                                                                                                                                                                                                             >  2:387423/1‑237 (MQ=255)
aGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAg                                                                                                                                                                                                                                             <  2:667302/239‑1 (MQ=255)
       tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTACCGACTGGGTGTCGGTGCAGGGAAGCACCaaaaagcca                                                                                                                                                                                                                                      >  2:530998/1‑231 (MQ=255)
       tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTGGTTGTTGGTACAGGGAAGCACCAAATCCCCa                                                                                                                                                                                                                                      >  1:433923/1‑239 (MQ=255)
       tCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGCTAAGCGACCGGGTGTTGGGACAGGTAAGCACCAAAACGCCa                                                                                                                                                                                                                                      >  2:1206744/1‑239 (MQ=255)
           tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTTGGGACCGGCAAGCACCAGAACGCCaaac                                                                                                                                                                                                                                   >  1:82946/1‑236 (MQ=255)
           tgctgcCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTCCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGcc                                                                                                                                                                                                                                  <  1:30353/239‑1 (MQ=255)
                ccTCGCGCGCTTTTTTTCCGAAATATAATTCTTGATAAATTTCCTCGTTTATTGGCTTTTCACCGCCGAAGCGATGCGCCATTTCGCGTACTTCTTTCGTATTGCGGTAGCCTTCCCCCACCTGCCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGcc                                                                                                                                                                                                                             <  2:966700/239‑1 (MQ=255)
                ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGACTGGTTGTCCGTACAGGGAAGAACCAGAACGCCCAAcccccc                                                                                                                                                                                                                              >  1:501866/1‑236 (MQ=255)
                ccTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTGTCACGCCTCGCTGTCGGCTCACGGCAGCACCTAACAGCCAAGcccccc                                                                                                                                                                                                                              >  2:273988/1‑236 (MQ=255)
                  ccgggcgcgTTTTTTCCGCAATATAATACTTGAGAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGCCTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCAt                                                                                                                                                                                                                           <  1:767203/235‑1 (MQ=255)
                     cgcgcgTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCCGAACGCCAAGCCCCGCCATga                                                                                                                                                                                                                         >  1:97778/1‑237 (MQ=255)
                                               ttGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCTACTGGTTGTTGGTACAGGTAAGCCCCAAAACGCCCAGCCCCGCCCTGCCCATAAAGGTGGCACGGTCGGCAcca                                                                                                                                                                                               >  1:557134/1‑237 (MQ=255)
                                               ttGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCCGCTGGTTGTCGGTACAGGGAAGAACCAGATCGCCCAGCCCCGCCATGCCCCTAAAGGTGGCAGGGGTGGCAccc                                                                                                                                                                                               >  1:985111/1‑238 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGGCTGGTTGTCGGGAAAGGGAAGGACCAAATCGCCAAACCCCGCCATGCCCACAAAGGGGGGAGGGTCGGCCCCCCgc                                                                                                                                                                                            >  1:186201/1‑239 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTGGTTGTCGGGACAGGGAAGAACCAGATCGCCAAGCCCCGCCCTGCCCATAAAAGTGGCAGGGTGGGCACCCCgc                                                                                                                                                                                            >  1:1161013/1‑239 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCACAACGCCCAGCCCCCCCATGCCCCCAAAGGTGGCAGGGTCGGGACCCac                                                                                                                                                                                             >  2:637378/1‑237 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAGATCCCCCAGCCCCCCCCTGCCCATAAAGGGGGCCGGGGCGGGCCCCAgc                                                                                                                                                                                            >  1:882197/1‑239 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAACACCAAAACCCCAAGCCCCCCCCTGCCCATAAAAGAGGGAGGGGCGGGACCCAg                                                                                                                                                                                             >  2:682614/1‑238 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTAAAGGTAAGCACCAAAACGCCAAACCCCGCCATGCCCCTAAAAGTGGCCGGGGCGGCACCCac                                                                                                                                                                                             >  1:743100/1‑237 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACCGGTTGTCGGTACAAGTAAGCCCCAAATCCCCCAGCCCCCCCCCGCCCCTAAAAGTGGCAGGGGCGGCccccct                                                                                                                                                                                             >  1:281495/1‑236 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCCACTGGTTGTCGGTTCAGGTAAGGACCAAATCGCCAAACCCCCCCCTGCCCCTAAAGGGGGCAGGGGCGGCACCCAg                                                                                                                                                                                             >  2:732277/1‑238 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGACTGGTTGTCGGTACAGGTAAGCACCAGAACGCCCAACCCCGCCATTCCCCTAAAGGGGGGAGGGTCGGCATCCac                                                                                                                                                                                             >  1:711061/1‑237 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCCGCTGGTTGTCGGGAAAGGTCAACACCAAAACCGCCAGCCCCCCCCAGCCCCTAAAGGTGGGCGGGGCGGCACCCac                                                                                                                                                                                             >  1:526344/1‑237 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCCACTGGTTGTGTGTAAAAGGCAGAGCCAGAAAGCTAACCCCCCCCCAGCCCCTAAACGTCGTATGGCAGGCACCCaa                                                                                                                                                                                             >  2:1125273/1‑237 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACACGACTTGTTTTCGGGACAGGGAAGCACCAAAACGCCAAACCCCCCCATGGCCCTAAAGGGGGCAGGGGCGGGACCCAgc                                                                                                                                                                                            >  1:427033/1‑239 (MQ=255)
                                                 gATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAACAGACTGGTTGTCGGTTAAGGGAAGCACCAGAACGCCAAACCCCCCCCAGCCCCTAAAGgggggcggcggcaccccgac                                                                                                                                                                                             >  2:244001/1‑222 (MQ=255)
                                                   tAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCCGCTGGTTGTCGGTACAGGTAAACACCAGAACGCCCAACCCCGCCCTGCCCATAAAGGGGGCAGGGGCGGCACCCAccg                                                                                                                                                                                           >  1:588266/1‑238 (MQ=255)
                                                   gaaaTTCCCGCGGTTAGTGCCTTTCAAACCCGAAAGTGAGGCCCCTGTTCGCTGACTTTTTTCTTGTTGCGGTCGCCCCCCCCCTCCTGCCCAGTCTGCTCCGGCGGGTTTTGTTCTCCCGTCCCTGGCCCAGGCCTCACGACAAACCGGCGTTTCCGCGCCGGGGTGCGGGTACAGGTAACCCCAAAATCACCACGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAgcgc                                                                                                                                                                                          <  2:248860/238‑1 (MQ=255)
                                                                ttATTGGCATTTCACCGCCGAAGCGATGCGCCAGTCCGCGGACTTCTTTCGTATGGCGGTAGCCTTCCACCACCGGACCAATCTCCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCAcc                                                                                                                                                                                                <  2:214218/220‑1 (MQ=255)
                                                                          ttCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGGAAGCACCAGATCGCCAAACCCCCCCCCGCCCCTAAAGGTGGCAGGGGCGGGACCCAGcgccccccctggctcttttacacat                                                                                                                                                                    >  1:214218/1‑217 (MQ=255)
                                                                           tCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTcagc                                                                                                                                                                 <  1:1103245/239‑1 (MQ=255)
                                                                                  cGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGGCCGGGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCCCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCa                                                                                                                                                          <  1:1125273/239‑1 (MQ=255)
                                                                                  cGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCa                                                                                                                                                          <  1:530998/239‑1 (MQ=255)
                                                                                             cgcCAGTTCGCGGACTTCTTTCGTATGGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCAGGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCa                                                                                                                                                <  1:1224288/239‑1 (MQ=255)
                                                                                                   ttCGCGGACTTTTTTCTTATTGCGGTAGCCTCCCCCCCCCTCACCATTCTTCTCCTGCGCTCTTTGTCCATCCTTGCCCTGCCCGAGCATCATGCCAAACCGGCGGTTACGCGCCTGGTTGTGGGTACAGGTAAGCCCCAGAGCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCg                                                                                                                                          <  2:108712/239‑1 (MQ=255)
                                                                                                     cgcgGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGCAAGCACCAAAACGCCAAACCCCCCCCCGCCCCTAAAGGGGGCAGGGGCGGGCCCCCACCCCGCCCCCAGACGCCGCATTTTCGCCAGCCCCCGGGGGGTCAGCCCCgg                                                                                                                                         >  2:419411/1‑237 (MQ=255)
                                                                                                             tcttTCGTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTCCGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                <  1:1206744/239‑1 (MQ=255)
                                                                                                             tcttTCGTATTGCGGTAGCCTTCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                <  1:732277/239‑1 (MQ=255)
                                                                                                             tcttTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAACCGAACGCAGGGCCGGACAGGCGAGCACCAGAACGGCAATCCCCGCCCTGCCCCCAACGGGGGCCGGGTCGCCATCCACCCCCCCCCCCACACGCGGCACATCTGCCAGCCCACCGGCGATCCGCGCCGCACGCGGAt                                                                                                                                 >  2:241186/1‑238 (MQ=255)
                                                                                                             tcttCCGTATTGCGGTAGCCTTCCCCCACCTGACCAATCTTCTCCGCCGCGCTTTGTCCATCCATGCCCTGCCCGACCATCATGCCAAAACGGCGGTTCCGCGACTGGTGGTCGGTACAGGTAAGCCCCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCAtt                                                                                                                                <  2:82946/239‑1 (MQ=255)
                                                                                                                   gTATGGCGGTAGCCTCCCACCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACACCCAGGCCCTGACCGAGCATCATGCAAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACAAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCAcc                                                                                                                          <  2:1161013/239‑1 (MQ=255)
                                                                                                                    tATTGCGGTAGCCTTCCCCCCCCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAgcg                                                                                                                                             <  1:735643/219‑1 (MQ=255)
                                                                                                                    tATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCCACCGGGTTTCGGTACAGGTAAACACCCAATCGCCAAGCCCCGCCCTGCCCCTAAAGGGGGCAGGGGCGGCACCCACCGCCCCCCCCCCACGCCACATTTCCGCCCGCCCACGGGGGCTCAGCGCCGCACGCGCCTTCGcccc                                                                                                                          >  2:110445/1‑238 (MQ=255)
                                                                                                                      ttGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTACCGGCTTGGTGTCGGGACAGGGGAGCACCAAATCACCAAGCCCCCCCATGCCCCTAAAGGTGGCAGGGGCGGCCCCCCGCGCCCCCCCCACACGCCACATTTCAGCCCACCCCCCGGGGGTCCGCGCCCCACCCGCCTTCGCCCCcaa                                                                                                                       >  2:920192/1‑239 (MQ=255)
                                                                                                                         cGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACCCGCCTGGTTGTCGGTACAGGTAAGCACCAAAACGCCCAGACCCCCCCCGCCCCTAAAAGGGGCAGGGGCGGGCCCCACCGCCGCCCCCAAACCCGGCCATTTAGCCCGCCCCCGCGGGCGCAGCGCCgccaccgccttcccccccaaac                                                                                                                     >  2:579834/1‑217 (MQ=255)
                                                                                                                            gcgCCTTCCCCCCCCTGACCACTCTCCTACTGCGCGCTTTGGACATCCGTGCCCTGCCCGAGCATCATGCCAAAACGGCGGTTTCGCGATTGTTGGCCGGTACGGAGAAGCCCCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGTTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGa                                                                                                                  <  2:172381/236‑1 (MQ=255)
                                                                                                                              gCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGCCTGGGTGTCGGTAAAGGTAAGAACCAAAACGCCAAACCCCCCCCTGCCCATAAAGGTGGGCGGGGGGGCCCCCCACGCCGCCCCCAGAACCGGCACTTCCATCAGCCCCCGGGGGATACGCGCCGGCCgcgccctgcctcttataaacacc                                                                                                                >  2:735643/1‑219 (MQ=255)
                                                                                                                              gCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTTGTTTTCGGTACAGGTAAGCACCACATCGCCCAGACCCCCCCAGCCCATTAAAGGGGCAGGGGGCGCCCCCCGCCCCGCCCCCAGCAGCGCCACTTCCGCCCAGCCCCGGGGGGTCAGCCCCGGACacgccactcccccccaacccaca                                                                                                                >  2:688544/1‑219 (MQ=255)
                                                                                                                              gCCTCCCACCCCCTGACCAATCTTCCCCTGCGCGCTGTGTACATCCATGCCCTGACCGAGCACCAGGCAAAACCGGCGGTTCCGCGACTGGTTGTCGTTCCAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATAc                                                                                                               <  2:400869/239‑1 (MQ=255)
                                                                                                                                     ccccccTGCCCAACCTTCTCCTGCGCGCTTTGTACACCCATGCCCTGCCCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGa                                                                                                       <  2:97778/235‑1 (MQ=255)
                                                                                                                                           ggACCAATCTTCCCCCGCGCGCTTGGTACACCCATCCCCGGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATccc                                                                                                 <  2:588266/238‑1 (MQ=255)
                                                                                                                                             ccaaaTCTTCCCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAACCGGCGGTTCCGCGACTGTTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCg                                                                                               <  1:682614/236‑1 (MQ=255)
                                                                                                                                             aCCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTTCGCGCCTGGTTTGCGGTACAGGGAAGCACCCGACCGCCAAGCCCCCCCCTGCCCCTAAAGGGGGCAGGGTCGGCACCCCGCGCCGCCCCCAGCCCCGCCATTTCCTCCCGCCCCCGGGCGGTCCCCGCCGCACGCGCATTCGCCCCAACACCGACACTGCCGGACCTcccc                                                                                                >  2:466317/1‑238 (MQ=255)
                                                                                                                                                    ttctCCTGCGCGCTTTGCACACCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAAt                                                                                        <  1:449295/239‑1 (MQ=255)
                                                                                                                                                         ctccgcgtTTTGTACACCCATCCCCTAACCGAGCATCATGCCAAAACGGCGGTTACGCGACGGGTTGTCGGTGCAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCaa                                                                                   <  2:957481/236‑1 (MQ=255)
                                                                                                                                                            cgcgcTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTAAGCGACCGGTTGTCGGTACAGGGAAACACCAAAACGCCAAACCCCCCCCCGCCCCTAAAAGGGGGAGGGGCGGCACCCAGCGCCGCACCCAAACGCCACACTTCAGCCAGCCCCCCGGGGGTCAGCGCCGCGCGCGCATTCGCACCACAACCCACACCCTCCGGCAACCCCCCACCCACGGCAaaaa                                                                                >  2:438514/1‑239 (MQ=255)
                                                                                                                                                                        acccttccccttcccGAGCACCATGCCAAAACGGCGTTTCCGCGACGGTTTGTCGGCACGGGTAAGCACCACTCCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACcg                                                                    <  2:882197/226‑1 (MQ=255)
                                                                                                                                                                             tGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACTCGCCTTGTTGTCGGGACAAGGAAGCACCAAAACGCCAAGCCCCCCCCCGCCCATAAAGGTGGCAGGGGCGGCCCCCCCCGCCGCACCCACACGCGCCCTTTCAGACAGCCCCCGGGGGAACCACGCCCCACTCCCCCTCTCACCAACACCGCCCCCCCCCGACATCCCCCCCCCCACCGCAATAACCGTTTTCACCCcgcc                                                                >  2:474131/1‑238 (MQ=255)
                                                                                                                                                                              gCCCTGACCGAGCATCATGCCAAACCGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCaa                                                                                   <  1:1186152/218‑1 (MQ=255)
                                                                                                                                                                                ccTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCA‑‑GTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAg                                                          <  1:244001/239‑1 (MQ=255)
                                                                                                                                                                                    aCCGAGCATCATGCCAAAACGGCGGTTACGCGGCTGGGTGTCGGTACAGGTAAGGACCAGATCGCCAAGCCCCGCCCACCCCCTAAAAGGGGCAGGGGCGGGACCCCCCGCCGCACCAAAACGCGCCATTTCCGCCACCCCCCCGGGGGTAACAGCCGTCAGCGCCTTCGCACCCAAACCCATAACGCCGGGCCACCCCCCCCCCACCAGCATAACGCTTTTCTCCCCgccgcccacc                                                         >  2:645411/1‑234 (MQ=255)
                                                                                                                                                                                       cagggacagtGCCAAAACGGCAGGTAGGCGAGTGGGTGGCGGTACGGGCAAGCACCAGCCCGCAAACCCCCGCCATGCCCATAAAGGTGGCAGGGGCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTg                                                       <  2:1066083/229‑1 (MQ=255)
                                                                                                                                                                                        aGCATCATGCCAAAACGGCGGTTACGCGACCGGTTGTCGGTACAGGGAAACACCAAATCGCCCAGCCCCCCCCTGCCCCCAAAGGGGGCCGGGGCGGCACCCCGCCCCCCACCCAAACGCCACATTTTAGCCCGCCCCCCGGGGATCAGCGCCCTACCCGCCTTCCCCCCCAAACCCAAACCCGCGGGCATCCCCCCCCCCACGGCCAAAACGTTTTTcctttttctttttcacctctc                                                    >  2:1186152/1‑219 (MQ=255)
                                                                                                                                                                                                    aaacCGGCGGTTACGCGACTGGTTGCCGGTACAGGTAAGCCCCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAAt                                         <  1:637378/239‑1 (MQ=255)
                                                                                                                                                                                                          gcggTTCCGCGACGGGTTGTCGGTACAGGGAAGCACCAGACCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGa                                    <  2:557134/238‑1 (MQ=255)
                                                                                                                                                                                                           gggTGACGCGACGGGTGGGCGGAACAGGTAACCCCCAGATCGCCAACCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGAt                                   <  1:466317/237‑1 (MQ=255)
                                                                                                                                                                                                           cgtttACGCGACTGGTTGTCGGTAAAGGTAAGCACCAGACCGCCAGGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGAt                                   <  1:115899/235‑1 (MQ=255)
                                                                                                                                                                                                                     aCTGGGTGTCGGGACAGGGAAGCACCAGATCGCCAAGCCCCCCCATGCCCATAAAAGGGGGAGGGGCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGACAGCCCCCGGGTGATCAGCGCCGTACGCGCACTCGCACCAAAACCCAAACCGTCGGACAACCCCCCCCCCATGGCAATAACGCTTTTCACCGCCCCGCCAAGCTGCACCCCCAGTAAATCCGGATTGCTGGAAAc                         >  2:1000458/1‑238 (MQ=255)
                                                                                                                                                                                                                              cGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACtt               >  1:692224/1‑239 (MQ=255)
                                                                                                                                                                                                                               ggTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACttt              >  1:573915/1‑239 (MQ=255)
                                                                                                                                                                                                                               ggTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACttt              >  1:663065/1‑239 (MQ=255)
                                                                                                                                                                                                                                   cAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc          >  2:133958/1‑239 (MQ=255)
                                                                                                                                                                                                                                    aGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGcc          >  2:937412/1‑238 (MQ=255)
                                                                                                                                                                                                                                           cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:865866/1‑239 (MQ=255)
                                                                                                                                                                                                                                           cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  1:176448/1‑239 (MQ=255)
                                                                                                                                                                                                                                           cACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgca  >  2:348616/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               
AGTAAAGTCAATGCTGCCTCGCGCGCGTTTTTTCCGCAATATAATACTTGATAAATTTCCTCGGTTATTGGCATTTCAACGCCGAAGCGATGCGCCAGTTCGCGGACTTCTTTCGTATTGCGGTAGCCTTCCACCACCTGACCAATCTTCTCCTGCGCGCTTTGTACATCCATGCCCTGACCGAGCATCATGCCAAAACGGCGGTTACGCGACTGGTTGTCGGTACAGGTAAGCACCAGATCGCCAAGCCCCGCCATGCCCATAAAGGTGGCAGGGTCGGCACCCAGCGCCGCACCAAGACGCGACATTTCAGCCAGCCCACGGGTGATCAGCGCCGTACGCGCATTCGCACCAAAACCGATACCGTCGGACATCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCA  >  NC_000913/3782678‑3783149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: