Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,862,669	T→A	28.3%	pseudogene (934/1617 nt)	glvC ←	pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,862,669	0	T	A	28.3%	45.1 / 9.9	57	pseudogene (934/1617 nt)	glvC	pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base T (24/10);  new base A (10/4);  total (38/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CATCATATCCGCGTGGTTGCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑GCGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAG  >  NC_000913/3862445‑3862904                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          catcatATCCGCGTGGTTGCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGTT‑TCGGG‑GAA‑C‑AGC‑AA‑ACCC‑G‑cc                                                                                                                                                                                                                                        >  2:442200/1‑238 (MQ=255)      tatCCGCGTGGTTGCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GTAAGGGT‑TGGGG‑AAA‑C‑ACC‑AACGCCC‑G‑CAC‑Cttt                                                                                                                                                                                                                                  >  2:403333/1‑239 (MQ=255)         ccggttGGTTGCTG‑ACATCCGGGATCCAGTTTGGCGAAAAAACCAAGTCAATCAGCCCCCCGCCCATGTGC‑‑CCCACCACACCAAAGAAATACTTTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTCCCGCAAAC‑AGCAACGGTAAAATGAACAGGAAGGTAAATTCC‑GGCGGTTCGGTAATCCCCACCAGCATGGC‑GT‑TTAAGGTT‑GCGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑cc                                                                                                                                                                                                                             <  2:923562/234‑1 (MQ=255)            cgTGGTTGCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGCGGG‑TTAAGG‑T‑GTGGG‑AAT‑C‑AGC‑CA‑CCCCAC‑CCC‑CTTTCA‑CCCg                                                                                                                                                                                                                           >  2:653615/1‑239 (MQ=255)              tGGTTGCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAAAGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGTT‑GCGGG‑GAC‑C‑AGC‑AA‑GCCC‑C‑CCC‑CCTTTT‑CCCGCtt                                                                                                                                                                                                                        >  1:1076523/1‑239 (MQ=255)                   gCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTTAGGTTGGCGGG‑AA‑‑C‑AGC‑‑A‑GCCC‑G‑CCACCTTTCC‑CCCGGTTTTCCg                                                                                                                                                                                                                   >  1:475769/1‑239 (MQ=255)                   gCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GTAAGGTT‑GGGGG‑GAT‑T‑AGC‑CA‑ACCC‑G‑CCA‑CCTTTA‑CACGTttttgcc                                                                                                                                                                                                                   >  1:470756/1‑236 (MQ=255)                        aCAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GTAAAGGT‑GCGGG‑GAT‑C‑AAC‑AA‑ACCC‑C‑CCA‑CTTTTA‑TCCCAtttttccgggcg                                                                                                                                                                                                              >  1:934176/1‑231 (MQ=255)                         acggcgggcacagggtgtGCGGTAAAACGAAAACAATCACACCGCC‑CCC‑TGTTGGCCCCACCACCCAAAACACAACAATTACGATCGATATGAGGGCCCCCGGCACGGCGTGCGCCGAAAAC‑AAAAGCAGTGAAATGAACGGAAAGGTAATTCCC‑AGCGGTTCGGTAATCCCCCCCACAATGGC‑GT‑TTAAGGTT‑GGGGG‑AA‑‑C‑AGA‑AA‑CCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCgg                                                                                                                                                                                                             <  2:475769/222‑1 (MQ=255)                                    cAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTTAAGGT‑GCGGG‑GAA‑T‑AGC‑AA‑ACCC‑GCCCA‑CTTTAA‑CCGGATTTCCGGGGGCgccgggaaaga                                                                                                                                                                                                    >  1:1233456/1‑229 (MQ=255)                                     aGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGGT‑GTCGG‑AAA‑C‑AAC‑AA‑ACCC‑G‑CCA‑ACTTTA‑CCCCATTTTTCGGTGCgcgactggagta                                                                                                                                                                                                   >  2:672722/1‑227 (MQ=255)                                            cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTTAAGGT‑GCGGG‑AAA‑C‑AGC‑AA‑GCCC‑C‑CCC‑CTTTTA‑CCCGCTTTTCCGGTGCGGCAGTGAAGGCCATCGCTa                                                                                                                                                                                           >  1:817127/1‑239 (MQ=255)                                            cGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGGT‑GCCGG‑GAT‑C‑AGC‑AA‑GACC‑G‑CCC‑CATTTA‑CCCCGATTTCCGGTGCGGCGGGGAAGGACATcccca                                                                                                                                                                                           >  1:1161620/1‑235 (MQ=255)                                                 aaaCCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑GCGGG‑GAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTTCGGTGCGGCAGTGGAGTACATCGCTAAAGaa                                                                                                                                                                                      >  1:337780/1‑239 (MQ=255)                                                         tCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGGT‑GGGGG‑GAA‑T‑AAC‑AA‑AGCC‑G‑CCC‑CCTTTA‑CCCGATTTTCCGGGGCGGCAGTGAAGTACAACGCTAAAAAAA‑AGCCCAc                                                                                                                                                                             >  2:654443/1‑239 (MQ=255)                                                             tCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGTT‑GGGGG‑GAT‑C‑AGC‑AA‑ACCC‑C‑CCC‑CCTTTT‑CCCCATTTTCCGGTGCGGCGGGGAAGTACAACGCCAAAAAAA‑AGCCCACGGc                                                                                                                                                                          >  1:893952/1‑238 (MQ=255)                                                                           atattG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GGTTTAAGGTT‑CGGGG‑AAT‑C‑ACA‑AA‑CCCC‑C‑CCC‑CTTTTC‑CCCG‑TTTTCCCGTGCGGCAGTGAAAGACATCGCCAAAAAAA‑AGACCCCGGCAcccacaagttttta                                                                                                                                                           >  2:572913/4‑227 (MQ=255)                                                                            tgtaaaggaaacagcacaaAAGAGAAAAATTACGGACGACATTGAGGCCGCCAGCACGGCGTGTACCCCAAAC‑AGCAACGGTGAAATGAACAGGAAGGAAAATCCC‑AGCGGTTCGGTAATCCCCACCAGCAGGGC‑GG‑TTAAGGTT‑GCGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTg                                                                                                                                                           <  2:600534/225‑1 (MQ=255)                                                                                g‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTTAAGGT‑GCGGG‑GAT‑A‑AGC‑AA‑ACCC‑G‑CCC‑CTTTTT‑CCCCCTTTTTCGGGGCGGCAGGGGAGGACATCGCCAAAGAAA‑TGACCACGGCACC‑AAAAATTTTTTAGTTa                                                                                                                                                      >  1:1183247/1‑238 (MQ=255)                                                                                      cacaacacaaagacatcaatggcGGTAGCCATGGAGGCCTCCAGCGGGGCGTGTGCCCCAAAC‑AACAAGGGGAAAAGGAAGAGGAAGATAAATCCC‑AGCGGTTGGGTAACCCCCCCCAGGAGGGG‑GG‑TTAAGTTG‑GGGGA‑AAA‑C‑AGC‑AG‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑g                                                                                                                                                <  2:1076523/217‑1 (MQ=255)                                                                                         acaccaAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑GCGGG‑GAT‑C‑AGC‑AA‑GCCC‑G‑CCC‑CCTTTATCCCCATTTT‑CTGTGCGGCCGGGGAGTACATCCCCAAAGAAA‑AACCCCCCGCCCC‑AAAGATTTTTTAGTTTCCCT‑Gccg                                                                                                                                             >  1:265052/1‑237 (MQ=255)                                                                                          caccaAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GTAAAGGT‑GCGGG‑AAT‑C‑AAC‑AA‑AACC‑C‑CCC‑CCTTTA‑CCCGCTTTTCCGCGGCGGCGGTGAGAGACAACGCCAAAAAAA‑AACCCACGGCCCC‑AAAAATTTTTTAGTTAcccggacag                                                                                                                                             >  2:996977/1‑232 (MQ=255)                                                                                                    aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGAT‑GCGGG‑AATGA‑AGC‑AG‑GCCC‑C‑CCC‑CTTTAC‑CCCGTTTTTCCGG‑GGGGCGGGGGAGAAAAAAGGCAAAAAAA‑AACCCAACGCACC‑AAAAATGTTTGAGATACCGC‑GC‑Agcggacaaacg                                                                                                                                  >  2:1237030/1‑228 (MQ=255)                                                                                                    aTACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GTTTGGGT‑GCGGG‑GAC‑A‑AGC‑AA‑ACCC‑C‑CCC‑CTTTTT‑ACCGATTTTTCTGTGCGGCGAGGAAGGAACACGCTTAAAAAA‑TTGCCACCGCCAC‑ACAGATGTTTTTGTTAACGT‑GCAAGGGCA‑AAAcc                                                                                                                                  >  2:370681/1‑238 (MQ=255)                                                                                                     tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GGTTTAAGGTT‑‑CGGG‑GAT‑C‑AGC‑CA‑ACCC‑C‑CCC‑CTTTTA‑CCCGGTTTTTCCGTGCGGCCGTGAAGTACAACGCTAAAAAAA‑AAACCACCGCCCC‑AAAAAATTTTTGGTTAACGT‑g                                                                                                                                                >  1:600534/1‑224 (MQ=255)                                                                                                     tACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGGT‑GCGGG‑GAT‑C‑AGC‑AA‑ACCC‑C‑CCC‑CTTTTA‑CCCCGTTTTCCCGGGCGGCAGGGAAGGACAACCCCAAAAAAA‑ATGCCACGGCACCAAAAGATTTTTTAG‑TACCCT‑GC‑AAGGCA‑AAAcccc                                                                                                                                >  1:884846/1‑236 (MQ=255)                                                                                                       cATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑GTAAAGGT‑GTGGG‑AAA‑A‑AGCAAA‑GCCC‑C‑CAC‑CTTTTA‑CCC‑AGTTTTCGGGGCGGGAGGGGAGGAACACGCCCAAGAAA‑AGACCACGCGCCC‑CAAGAATTTTTAGTTGACGG‑GC‑AGGGCA‑AAAcccgcc                                                                                                                              >  2:781763/1‑234 (MQ=255)                                                                                                            cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑GCGGG‑GAT‑A‑AGG‑AA‑GCCC‑G‑CCC‑CCTTTA‑CCCCGTTTTTCGGTGCGGCCGGGAAGGAAATCGCTCAAAAAA‑AACCCCCCGGACC‑CAAGAGTTTTTAGGTACCCTGGC‑CGGGAA‑AAACGCCCTTCCgg                                                                                                                         >  2:223641/1‑239 (MQ=255)                                                                                                            cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGGT‑GCGGG‑GAT‑C‑AGG‑AA‑ACCC‑G‑CCC‑CCTTTT‑CCCGCTTTTTCGGTGCGGGAGTGGAGTACATCGCTAAAGAAA‑AGCCCCCGGCACC‑CAAAAATTTTTAGTTACCGT‑GC‑AGGGCA‑AAAACGCCCTCCggg                                                                                                                        >  1:118564/1‑239 (MQ=255)                                                                                                            cGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGGT‑GCGGG‑GAT‑C‑AGC‑AA‑ACCC‑G‑CCA‑CTTTTA‑CCCCATTTTCCGGTGCGGGCGGGAAGTACATCCCTAAAGAAA‑TTCCCACCGCACC‑ACAGATTTTTTAGTTACCGT‑GC‑AGGGCA‑AAAACGCCTTCCgcg                                                                                                                        >  1:230200/1‑237 (MQ=255)                                                                                                               tCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑GCGGG‑GAT‑C‑AAC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTTAGTTACCGG‑GC‑AGGGCA‑AAACCGCCTTCCGGGgac                                                                                                                     >  1:379086/1‑239 (MQ=255)                                                                                                                  aCATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGGT‑TCGGG‑AAT‑C‑AGA‑CA‑GACC‑G‑CCC‑CATTTT‑TCCGCTTTTTCCGTGGGGCAGGGGAGGAAACTCCCCAAGAAA‑ATCCCACCGCACC‑ACAGACTTTTTTGTTTCCCG‑GC‑CGCGCA‑ACAACCCCCCCCCGGaacaac                                                                                                                  >  2:204889/1‑239 (MQ=255)                                                                                                                             gccAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑GCGGG‑GAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTT‑CCCCCTTTTTCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑AGCCCACGGCACC‑CAAGATTTTTTAGGTACCCT‑TC‑AGGGCA‑AAACCCCCTTCCCGGAACAACGATTTctacc                                                                                                        >  1:67557/1‑234 (MQ=255)                                                                                                                                    cGGGGTGTACCCCAAACAAGCAACGGAAAAAGGAACAGGAAGGTAAATCCC‑AGCGTGTCGGTACCCCCCACCACCAGGGC‑GG‑TTAGGGTT‑GCGGA‑AAC‑C‑AGC‑AA‑CCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTc                                                                                                    <  2:265052/236‑1 (MQ=255)                                                                                                                                       cttttACCGCAAAC‑AGCCACGGTAAAATGAACAGAAAGTTAAATTCC‑AGCTTTTCGTTAATCCCCCCCAGCATGGC‑GT‑TTAAGTTT‑CCGGG‑AAT‑C‑ACC‑AA‑CCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCt                                                                                              <  1:1179218/234‑1 (MQ=255)                                                                                                                                               gaaaaC‑AGAAACGGTAAAAGGAACAGGAAGGAAAATCCC‑AGGGGTTCGGTAATCCCCCCCAGCGTGGC‑GG‑TTAAGGTT‑GCGGG‑AAC‑C‑AGC‑AA‑CCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGaa                                                                                     <  1:442200/237‑1 (MQ=255)                                                                                                                                                cAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGGT‑GCGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTT‑ACCGAATTTTCGGGGGCGCAGTGGAAGACATCACTAAAAAAA‑TTCCCACCGGAAC‑CAAGAATTTTTAGTTAACCG‑GC‑AGGGCA‑AAAACGCCTTCCCGGGACAAAGAGTTCaaggggccggcgacccaaacgaa                                                                                     >  2:727922/1‑216 (MQ=255)                                                                                                                                                cAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGTT‑GCGGG‑AAT‑C‑AGC‑AA‑GACC‑G‑CCA‑CTTTTA‑CCCGCATTTCCGGTGCGGCCGGGAAGTACCTCGCTAAAACAA‑TGCCCCCGGCCCC‑CCAGTCTTTTTAGTTACCGT‑GC‑CGGGCACAAACCCCCTCCCGGGACAAACGttttaaccgctccgcgccccaaccgga                                                                                      >  2:702671/1‑215 (MQ=255)                                                                                                                                                cAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGGT‑GCGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CCTTTA‑CCCCCTTTTTCCGTGCGGCAGTGAAAGACACCGCTAAAAAAATTACCCAC‑GCCCC‑AAAGAATTTTTAGGTAGCCC‑GC‑AGGGCA‑AAACCCCCCTCCCGCGACAAAGATTTCAGCcgcgccgcgccccaaacgaa                                                                                     >  2:640172/1‑228 (MQ=255)                                                                                                                                                        cAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGGT‑TCGGG‑AAA‑C‑AGC‑AA‑GCCC‑C‑CCA‑CTTTTA‑CCCCATTTTCCGGTGCGGCAGTGAAATACATCCCTAAAGAAA‑TGCCCACGGCACC‑AAAAATTTTTTAGTTACCGT‑GC‑AGGGGA‑AAAACCCCCTCCGGGAACAACGATTTCAGCGGCCCCCCGCTCCAACTGAACCCTTTCa                                                                             >  2:1208360/1‑239 (MQ=255)                                                                                                                                                               aaagggAACGGGAGGGTAACTTCC‑AGTGGCGCGAGACTCACCACAGGCAGGGC‑GG‑TGAGGGTT‑GGGGT‑AAG‑A‑AGA‑AA‑GGCC‑G‑CCA‑CGTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGACCTCTTGCAGAtgct                                                                       <  2:1242715/233‑1 (MQ=255)                                                                                                                                                                 aaagAACAGGAA‑GTAAATTCCGGGCGGCTCGGTAACTCCCCCCAGCATGGC‑GG‑TAAAGGTG‑GCGGG‑AAT‑C‑ACC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                    <  1:573248/236‑1 (MQ=255)                                                                                                                                                                 aaTGAACAGGAAGGTAAATTCC‑AGGGGTTCGGTAATTCCCCCCAGCATGGC‑GG‑TTAAGGTT‑GGGGG‑AAA‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTgcg                                                                    <  1:572913/239‑1 (MQ=255)                                                                                                                                                                   tGAACAGGAAGATAACTTCC‑AGGGTTCCGGTAATCCCCACCAGCTTGGC‑GG‑TTAGGTTT‑GCGGA‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGcc                                                                  <  1:654443/239‑1 (MQ=255)                                                                                                                                                                      aCAGGAAGATAAATCCC‑AGCGGTTCGGTAATCCCCCCAAGCATGGC‑GG‑TTAGGTTG‑GGGGG‑AAA‑C‑AGA‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAg                                                               <  1:314736/239‑1 (MQ=255)                                                                                                                                                                        aGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGAT‑TCGGG‑AAA‑T‑AGC‑AA‑GCCC‑G‑CCC‑CTTTTA‑CCCGATTTTTCGGTGCCGCAGGGaa                                                                                                                                                                                                      >  2:298351/1‑107 (MQ=255)                                                                                                                                                                         ggagagTAAATTCG‑GGCGGTTCGATAACCCCCCCCAGCAGGGG‑GG‑TAAGGGTG‑GGGGA‑AAA‑C‑AGA‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTa                                                             <  2:1234551/233‑1 (MQ=255)                                                                                                                                                                          gAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAAGTT‑GCGGG‑AAA‑C‑AGC‑AA‑GCCC‑C‑CCA‑CTTTTA‑CCCGATTTTTCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACCGCCCC‑AAAGATTTTTTAGTTACCGC‑TG‑AGGGCA‑AAACCGCCCTCCCGGAACAACGATTTCAGCGGCTCGGCGCCCCAACTTAACTCCTGCAGAAGCTGCCCCCAgcaa                                                            >  2:807529/1‑235 (MQ=255)                                                                                                                                                                                   atccgggCGGTCAGGTACTCCCCACCGGCATGGG‑GG‑TAAAGGTT‑GC‑GG‑AAT‑CAAAC‑AC‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATg                                                  <  1:177743/233‑1 (MQ=255)                                                                                                                                                                                        aGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTTAGGTT‑GCGGG‑AAG‑A‑AGG‑AA‑GACC‑C‑CCA‑CTTTTA‑CCCGACTTTCCGGGGGGGCCGGGAAGTAGCACGCTTAACAAA‑ATCCCCCGGGCCC‑AAAGAATTTTGTGTTTCCGT‑TC‑AGGGCC‑AAACGGCCCTCCGGGAACAACATTTTCAGCGGGTCGGGGTCAAAACTCAACTCTTGCCAGTGCTCCGCCCCGCA‑CCACTGACTgcacc                                              >  2:273552/1‑235 (MQ=255)                                                                                                                                                                                         gCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑TCGGG‑AAT‑C‑AGC‑AA‑ACCC‑G‑CCC‑CTTTTT‑CCCGATTTTTCGGTGCGGCCGTGAAGTACATCGCTCAAAAAA‑TGCCCCCGGCCCC‑CAAGATTTTTGAGGTACCCT‑GC‑AGGGCA‑AAAACGCCTTCCGGGGACAAAGATTTTCGAGGCTCCGCGCCCCAAACAACCTCCTTCCGATGCTGCGCCCCATC‑CCTCTGAAAgcccc                                              >  2:229027/1‑235 (MQ=255)                                                                                                                                                                                                         caccaGCAGGGC‑GG‑TTAAGGTT‑GGGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATGCCGCCTTCAACAGCTGCCGGAc                            <  1:807529/239‑1 (MQ=255)                                                                                                                                                                                                           ccgtgcgggC‑GT‑TTAGGGTG‑GGGGG‑AAC‑C‑AGC‑AG‑CCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATGCCGCCTTCAACAGCTGCCGGACCa                          <  2:884846/232‑1 (MQ=255)                                                                                                                                                                                                            caGCATGGC‑GG‑TTTAGGTT‑GCGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCC‑CTTTTA‑CCCGATTTTTCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCCCC‑CAAGATTTTTTAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCCGCGCTCCAACTGAACTCTTTCAGATGCCGCGCCCCGTACCACCTG‑ATGGCGCCTTCCACAACTGCCCGAccca                         >  2:1046543/1‑237 (MQ=255)                                                                                                                                                                                                                           aaGGGT‑GGGGGAAAT‑C‑AGA‑AA‑CCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTc             <  1:781763/239‑1 (MQ=255)                                                                                                                                                                                                                                 ggCGGG‑AAT‑G‑CGC‑AC‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAg        <  1:572222/237‑1 (MQ=255)                                                                                                                                                                                                                                        aaT‑C‑AGC‑AA‑CCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTCTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAg  <  1:265365/238‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                          CATCATATCCGCGTGGTTGCTGAACAT‑CGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTG‑‑CCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAAC‑AGCAACGGTGAAATGAACAGGAAGGTAAATTCC‑AGCGGTTCGGTAATTCCCACCAGCATGGC‑GG‑TTAAGGTT‑GCGGG‑AAT‑C‑AGC‑AA‑GCCC‑G‑CCA‑CTTTTA‑CCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAA‑TGCCCACGGCACC‑AAAGATTTTTGAGTTACCGT‑GC‑AGGGCA‑AAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCGGCGCTCAAACTGAACTCTTGCAGATGCTGCGCCCAGTA‑CATCTGAATGCCGCCTTCAACAGCTGCCGGACCAAAGATAAACTGTCCGTAGATGAAG  >  NC_000913/3862445‑3862904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑