BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,864,958	T→C	18.4%	I439V (ATC→GTC)	yidE ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,864,958	0	T	C	18.4%	269.8 / 12.3	112	I439V (ATC→GTC)	yidE	putative transporter
Reads supporting (aligned to +/- strand): ref base T (51/37); new base C (2/22); total (53/59)
Fisher's exact test for biased strand distribution p-value = 1.42e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TTAGCAAACGCCAGCGCCGGAGGATCGGTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGTTGCAGTTTTTGCTGCGCATTCCCC  >  NC_000913/3864732‑3865196

ttaGCAAACGCCAGCGCCGGAGGATCGGTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAACTTAGCCCTTCGCCCTTGACCAGGGGATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGCGAGGAACA‑GCACGATCCCCAGCcccc                                                                                                                                                                                                                                      >  2:21329/1‑238 (MQ=255)

   gCAAACGCCAGCGCCGGAGGATCGGTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAAACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCa                                                                                                                                                                                                                                   >  1:290016/1‑238 (MQ=255)

           cAGCGCCGGAGGATCGGTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGt                                                                                                                                                                                                                          <  1:269467/239‑1 (MQ=255)

                       aTCGGTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTgg                                                                                                                                                                                                               <  2:973389/237‑1 (MQ=255)

                       aTCGGTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTgg                                                                                                                                                                                                               <  1:377812/237‑1 (MQ=255)

                          gttCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCGGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCGTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCa                                                                                                                                                                                                          <  2:908348/237‑1 (MQ=255)

                          ggTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCa                                                                                                                                                                                                          <  2:234426/239‑1 (MQ=255)

                          ggTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCa                                                                                                                                                                                                          <  2:216622/239‑1 (MQ=255)

                          ggTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCa                                                                                                                                                                                                          <  2:1195194/239‑1 (MQ=255)

                          ggTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCa                                                                                                                                                                                                          <  1:268123/239‑1 (MQ=255)

                             cATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCAGTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATaa                                                                                                                                                                                                       <  2:1050106/239‑1 (MQ=255)

                                      tGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCCGGGCACCATAACCAAT‑CCAGCTTAACCCTTCGCCATTGACCAGGGGATTC‑CCAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCCCCCGCAGCGCCAGGTTGGCG‑CCTGGCGGCCTAAACCaacac                                                                                                                                                                                               >  2:464679/1‑234 (MQ=255)

                                       gCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAg                                                                                                                                                                                             <  1:148806/239‑1 (MQ=255)

                                              tCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACTAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTtgccg                                                                                                                                                                                      <  1:687880/239‑1 (MQ=255)

                                               cccGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGGACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCCAGGGATTC‑ACACAAAACCCACCAGCTTTCAGACCAACGACCGAGAGGAACA‑GCACCACCCCCCGCTCCCCCAGCGCGAGGGTGGCG‑CCTGGCGGCATAAACCAGTACAGCTtgcc                                                                                                                                                                                       >  2:774430/1‑237 (MQ=255)

                                               cccGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTtgccga                                                                                                                                                                                     <  2:789387/239‑1 (MQ=255)

                                                ccGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGGATTC‑ACAAAATCCCCACCCGATTTCAGACCAACGACCGAGAGGAACA‑GCCCGATCCCCAGCTCCCGCAGCGCGAGGGTGGCG‑GGTGGCGGCATAAACCAGGAAAGATtgccgat                                                                                                                                                                                    >  2:64042/1‑239 (MQ=255)

                                                        ggTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATcc                                                                                                                                                                                                                                               <  1:183850/173‑1 (MQ=255)

                                                        ggTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTTGCG‑CTTGGCGGCATAAACCAGTACAGCTtgccgatactgccga                                                                                                                                                                            >  1:1144532/1‑239 (MQ=255)

                                                         gTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTACAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGAACAGCTtgccgatactgccga                                                                                                                                                                            <  1:1019282/238‑1 (MQ=255)

                                                               tAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCCGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑CCAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGc                                                                                                                                                                       <  1:647498/237‑1 (MQ=255)

                                                                aattaaggttGGGTAACATCCGCGCCAGAATGCCAACAGTAATGAGCAG‑AACGGCGTTAATCAGGGCAACATAACCAAA‑ACAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACAGAGAGGATCA‑GCACGGTCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CATGGCTGCATAAACCAGTACAGCTTGCCGATACTGCCGATGCGCCCg                                                                                                                                                                    <  2:873046/231‑1 (MQ=255)

                                                                aaTTCATTTTGGCTAACATCCGCGCCGGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCg                                                                                                                                                                    <  2:355147/239‑1 (MQ=255)

                                                                  ttCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCACCTTAACCCTTCGGCATTGACCCGGGGATTC‑AAAAAATCCCCACCAATTTTCCGGCCCAAGGCCGAGAGAAGCA‑GCACGATCCCCAGCCCCCGCCGGCCGCGGTTGGCG‑CTTGGGGGCATAAACCCATACAGCGTGCCGACTCTCCCGGTCCGCCCggg                                                                                                                                                                  >  2:196595/1‑237 (MQ=255)

                                                                  ttCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAA‑CCAGCTTAGCCCTTCGCCATTGACCAGGGGATTC‑CCCAAATCCCCACCCGATTTCAGACCAACGACCGAGCGGAAAA‑GCACGACCCCCAACCCCCg                                                                                                                                                                                                                                     >  2:183850/1‑173 (MQ=255)

                                                                     aTTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGac                                                                                                                                                               <  1:357864/239‑2 (MQ=255)

                                                                       tttGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCa                                                                                                                                                             <  1:1147717/239‑1 (MQ=255)

                                                                         tGGCTAACATCCGCGCCAGAATGCCAACAGTAACCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTA‑ACAAAATCCCCACCAGATTCCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAAc                                                                                                                                                           <  1:1038194/239‑1 (MQ=255)

                                                                                cATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATaa                                                                                                                                                    >  1:531445/1‑239 (MQ=255)

                                                                                   ccGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCa                                                                                                                                                 <  1:64042/239‑1 (MQ=255)

                                                                                     gcgcCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGAGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGGATTC‑ACAAAAACCCCACCAGATTTCAGACCAACGAACGAGAGGAAAA‑GCACGATCCCCAGCTCCCGCAACGCGAGGGTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGACACGCCCGAGGAGCAAAGCCATAATCAGc                                                                                                                                               >  2:201510/1‑239 (MQ=255)

                                                                                                  aacgagaaacAGCGGAAAGGGGGGTGATCGGAGCCCAATACCAAAG‑CCAGCTTAGCCCTCCGCCATTGACCGGGGTATCC‑AAAAAACCCCCCCAAGATTGCAGACCAACGACCGAGAGGAACA‑GCACGACCCCGAGCCCCCGCGGCGCGAGGTGGGCG‑CTGGGCGGAATAAACCAGTCCAGCTTGCCGATACTGCCGATCCGCCCGAGGACCAACGCAAAAATCAGCGGTCcgtccgcc                                                                                                                                   <  2:866629/230‑1 (MQ=255)

                                                                                                        aagcAGCGG‑ACCGGCGGTGAGAGGGGCA‑CAAAACCAATCCCAGCTAGGCCCTTCGCAATGGCCCAGGGTATTC‑AAAAAACCCCCCCCAGATTCAAGACCAACGCCGGAGAGAAACA‑GCACGACCCCCAGCTCCCGCAGGGCGGGGTGGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGccc                                                                                                                              <  2:1189807/234‑1 (MQ=255)

                                                                                                              cGG‑AACGGCGGTGGTCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCCTTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGAACAACGACCGAGAGGGACA‑GAACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGAACAGCTTGCCGATACTGCCGAAACGCCCGAGGATCAACCCACTCAACAGCGGTCCGCCCCCCCGCCCCCGATTCa                                                                                                                      >  2:905967/1‑239 (MQ=255)

                                                                                                              cGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCa                                                                                                                                                                                                          >  1:1164148/1‑155 (MQ=255)

                                                                                                              cGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTGCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCa                                                                                                                                                                                                          <  2:1164148/155‑1 (MQ=255)

                                                                                                                         gTGATCCGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGCGGATCAACGCCCTAATCAGCGGTCCGCCCGCCAGCCCCCGTTTCAACGCGGCCgg                                                                                                            >  1:618786/1‑239 (MQ=255)

                                                                                                                                 ggCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCCGTTTCAACGCGGCCGGGAATCCTg                                                                                                    >  1:631823/1‑239 (MQ=255)

                                                                                                                                 ggCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTg                                                                                                    >  2:86158/1‑239 (MQ=255)

                                                                                                                                 ggCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTg                                                                                                    >  1:888023/1‑239 (MQ=255)

                                                                                                                                   cACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTgg                                                                                                   >  2:31329/1‑238 (MQ=255)

                                                                                                                                    aCCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGccc                                                                                                                              <  1:331822/210‑1 (MQ=255)

                                                                                                                                    aCCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGccc                                                                                                                              >  2:331822/1‑210 (MQ=255)

                                                                                                                                      cATAAGCAAT‑CCAGCTTAACCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGCGAGGAACA‑GCGCGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTCCAGCTTGCCGCTACTGCCGATACGCCCCAGGATCAACGCCATAATCAGCGGTCCGCCCGCCGGCCCCACTTTCAACGACGCCCGGAATCCGGGcaca                                                                                               >  2:114662/1‑239 (MQ=255)

                                                                                                                                      cATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGcaca                                                                                               >  1:1202847/1‑239 (MQ=255)

                                                                                                                                      cATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGACGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCACCCCCAGTTTCAACGCGGCCGGGAATCCTGGcaca                                                                                               >  2:493360/1‑239 (MQ=255)

                                                                                                                                      cATAACCAAT‑CCAGCTTAGCCCTCCGCCATTGACCAGAGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGCGGAACA‑CCACGATCCCCAGCTCCCGCAGCGCGAGTTTGGAG‑CTTGGCGGCATAAACCAGTACAGATTGCCGATACTGCCGATACGCCCGAGGAGCAACGCCATAATCAGCGGCCCGCCCGCCAGCCCCAGCTTCAATGCGTCCGGGGATCCCTGcaca                                                                                               >  2:939277/1‑239 (MQ=255)

                                                                                                                                      aaTACCCACT‑CCGGCTGAGCCCTCCCCCTTGGACGAGGGAATTC‑ACAAACTCCCCACAAGATTCAAGACCAACGACCGAGAGGAACG‑GCACGACCCCCAGCTCCCGCAGGGCGGGGTGGTCG‑CTTGGGGGCATAAACCAGTCCAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGcaca                                                                                               <  2:232846/238‑1 (MQ=255)

                                                                                                                                          acaaaT‑CCAGCTTAGCCCTTCGCCATTGACCGGGGTATTC‑ACAAACTCCCCCCCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTGGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGG‑TCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGAc                                                                                          <  1:1148047/236‑1 (MQ=255)

                                                                                                                                           ccAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGCGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGAc                                                                                          >  1:162316/1‑239 (MQ=255)

                                                                                                                                           ccAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGAc                                                                                          <  1:86158/239‑1 (MQ=255)

                                                                                                                                           ccAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGAc                                                                                          >  2:194959/1‑239 (MQ=255)

                                                                                                                                               t‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGa                                                                                      >  2:957444/1‑239 (MQ=255)

                                                                                                                                                 ccggCTAAGCCTTTGCCCATGGCCCAGGGTTTCC‑CAAAAACCCCCACCAGATTCCAGACAAACGACCGAAAGGAACA‑GCACGCCCCCCTCCTCCCGAGGCGGGAGGTTGGCG‑CGTGGGGGCATAACCCAGTACAGCTTGCCGATCCTGCCGATCCCCCTGAGGATCAAGGCCATAATCAGCGGCCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGaa                                                                                     <  2:22041/236‑1 (MQ=255)

                                                                                                                                                 ccAGGTTCGCCCTTCCGCATTGCAGAGGAAATTC‑ACAAAACCCCCCCCAGATCGCAGACCAACGACCGAGAGGAACA‑GCACGACCCCCAGCTCCCGCACGGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGaa                                                                                     <  2:1227879/239‑1 (MQ=255)

                                                                                                                                                 ccAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAg                                                                                                                                                                                             <  1:991655/135‑1 (MQ=255)

                                                                                                                                                 ccAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGaa                                                                                     >  1:271060/1‑239 (MQ=255)

                                                                                                                                                 ccAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCAAAAAGACGGGaa                                                                                     >  1:1080547/1‑239 (MQ=255)

                                                                                                                                                 ccAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGATTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGaa                                                                                     >  1:923426/1‑239 (MQ=255)

                                                                                                                                                 ccAGCTGACCCCTCCCCTATTCACGAGGGTCTAC‑ACAAAACCCCCCCCACATTCCACCCCATGCGCCGAGAGGAACC‑GCACCCCCCCCAGCACCCGCAGC‑CGTGGGTGGCGACTTGGCGGAAAAACCCAGTCCACCTTGCCGTTACTGCCGAGCCGCCCGAGGAACACCGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGaa                                                                                     <  2:65394/239‑1 (MQ=255)

                                                                                                                                                     cTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGCCGGGACTAgc                                                                                 >  2:252232/1‑238 (MQ=255)

                                                                                                                                                     cTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGa                                                                                 >  1:877282/1‑239 (MQ=255)

                                                                                                                                                     cTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGCCCGGGAATCCTGGCACAAAGACGGGAATAGa                                                                                 >  1:652951/1‑239 (MQ=255)

                                                                                                                                                          cctcccgacATGGACAAGGGTATGC‑ACAACACCCCCCCAAGTTTAAAACCCAAGGACGGAGAGAAACA‑CCACGACCCCAGGCTCCCGCAGCGCAAGTGTGGCG‑TTTGGCGGAAAAAACCAGTACAGCTTCCCGATCCTGCCGATACGCCAGAGGACCAACGCCATAATCGGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGTGAATCCTGGCACAAAGACGGGAATAGAACCTa                                                                            <  2:144997/231‑1 (MQ=255)

                                                                                                                                                          cccTTCCCCATCGACCGGGGTTGTC‑ACAACACCCCCCCCAATTTCCAGACCACCCACCGAGAGGAACA‑GCACGACCCCCAGCCCCGCCCGCGGGGGGTTGGCG‑CTTGCCGGCACAACCCAGTCAACCTTGCCATTACCGCCGATCCGCCCGGGGATCACCGCCATAATCAGCGGCCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCt                                                                             <  2:1020668/238‑1 (MQ=255)

                                                                                                                                                          acctgctccATTAAACAGTGTATTC‑ACAAAACCCACACCAGATCGTAGCACACCGACGGAGAGAAACG‑ACCCTTCCCCCGTCCCCCGCTGCTGTAGGTTGCCG‑TCTGGCGGCATAACCCATTACACCTTCCCGATACTGCCGATCCGCCGGAGTAACAACGCCATAACCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCt                                                                             <  2:60149/231‑1 (MQ=255)

                                                                                                                                                           cctgccccATTG‑CCAGGGTATACAACAAAAACCCCCCCGGATTGCAAACAACCGACAGAGGGGAACA‑GCCGAACCCCCAGCCCCCGCGGGGCGGGGTTGGCG‑TTTGGCGCCAAAACCCATTACAGCTCGCCAATACGGCCGATACGCCGGAGGACAAACGCCATAACCAGCGGCCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCt                                                                             <  2:618786/231‑1 (MQ=255)

                                                                                                                                                           ccTCCGCCATTGACCAGGGTATTC‑ACAAAACCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGACCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACTGGAATAGAACCt                                                                             <  1:65255/237‑1 (MQ=255)

                                                                                                                                                               cGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTtgccga                                                                                                                                                                                     >  1:1036687/1‑129 (MQ=255)

                                                                                                                                                               cGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTtgccga                                                                                                                                                                                     <  2:1036687/129‑1 (MQ=255)

                                                                                                                                                               cGCCATTGACCAGGGTATCC‑AAAAAACCCCCACCAGATTCAGGCCCAACGACCGA‑AGGGACACGCCGGACCCCCAGCCCCCGCGGCGGGAGGTGGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATCCGCCGGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAAt                                                                       <  2:1176085/239‑1 (MQ=255)

                                                                                                                                                                            ggTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACACTACGCCTACCCCGa                                                          >  2:525898/1‑239 (MQ=255)

                                                                                                                                                                            ggTATTC‑AAAAAACCCCCCCCAGCTTTCAGCCCAACGACCGAGAGGAACG‑GCACGATCCCCAGCCCCCGCGGGGCGAGTTTGGCG‑TTTGGCGGCATAACCCAGTCCAGCTTGCCGATCCTGCCGATCCGCCCGAGGACCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAAt                                                                       <  2:691148/226‑1 (MQ=255)

                                                                                                                                                                             gTAGTC‑ACAACACCCCCCCCAGATTCGAAACCAACGACCGAGAGGAACA‑GCACGACCCCCAGCTCCCGCAGCGCGAGGTGGGCC‑GTTGGCGGCAAAAACCAGTACAGCTTCCCGATACGGCCGATACGCCCGAGCATCAACGCCATAATCAGCGGCCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGa                                                          <  2:155389/238‑1 (MQ=255)

                                                                                                                                                                              gctgaccaaaaaCCCCCGCCAGATTCCAGACAAACGCCGGAGAGGACCA‑GCACGACCCCCAGCCCCCGCAGCGCGGGGTGGGCG‑CTTGGGGGCATAAACAAGTACAGCTGGCCGATCCTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGa                                                          <  2:786975/230‑1 (MQ=255)

                                                                                                                                                                                    acaAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAAt                                                   >  1:440029/1‑239 (MQ=255)

                                                                                                                                                                                     caAAACCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGACCCCCAGCCCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATa                                                  <  1:1152133/239‑1 (MQ=255)

                                                                                                                                                                                       aaaTCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGcc                                                             >  1:691148/1‑226 (MQ=255)

                                                                                                                                                                                       aaaTCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATaa                                                 >  1:199408/1‑238 (MQ=255)

                                                                                                                                                                                       aaaTCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATaa                                                 >  1:786975/1‑238 (MQ=255)

                                                                                                                                                                                       aaaCCCCCACCAGATTGAAGACAACCGACGGAGGGAAGCA‑GAACGACCCCCAGCGCCCGCAGCGGGAGGTTGCCG‑CTGGGCGGCAGAAACCAGACCAGCTTGCCGATACGGCCGAGACGCCCGAGGATAAACCCCAGAATAAGCGGCCCACCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATaaa                                                <  2:212337/239‑1 (MQ=255)

                                                                                                                                                                                           ccccccCAGATTCCAGACAAACGACCGGGAGAACAA‑CCACGACCCCCAGCCCCCGCGGCGCGGGGTGGCCG‑CTGGGGGGCAAAACCAAGACCAGCTTGCCGTTCCTGCCGATCCGCCGGGGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACt                                            <  2:531445/239‑1 (MQ=255)

                                                                                                                                                                                           ccccACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAac                                               >  1:23159/1‑236 (MQ=255)

                                                                                                                                                                                              caccaGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGgc                                         >  2:428846/1‑239 (MQ=255)

                                                                                                                                                                                              caccaGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCAAGCTCCCGCAGCGCGAGGTGGGCG‑CTGGGCGGCATAAACCAGTCCAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGgc                                         <  1:890742/239‑1 (MQ=255)

                                                                                                                                                                                                      ttCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTg                                 >  1:68327/1‑239 (MQ=255)

                                                                                                                                                                                                              aaCGCCCGGGAGGAACA‑GCACGACCCCCAGCTCCCGCAGCGCGAGGTGGGCG‑CTGGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGtt                         <  1:306064/239‑1 (MQ=255)

                                                                                                                                                                                                              aaCGACGGAGAGGAACA‑GCCGGATCCCCAGCTCCCGCAGCGCGAGGTGGGCG‑TTTGGCGCCATAAACCAGTACAGCTTGCCAATCCTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGtt                         <  1:774430/239‑1 (MQ=255)

                                                                                                                                                                                                              aaCGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCAAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGtt                         >  2:609535/1‑239 (MQ=255)

                                                                                                                                                                                                               aCGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGtt                         >  1:711113/1‑238 (MQ=255)

                                                                                                                                                                                                                cgcccggggggAACG‑CCACCACCCCCCGCCCCCGCAGCGCGAGTTGGGCG‑CTTGGCGGCATAACCCAGAGCAGTTTGCCGTTACGGCCGATCCGCCGTAGGACCAACGCCATAACCAGCGGCCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGTTGc                       <  2:631823/230‑1 (MQ=255)

                                                                                                                                                                                                                cGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGa                                                          <  1:1065417/204‑1 (MQ=255)

                                                                                                                                                                                                                cGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGa                                                          >  2:1065417/1‑204 (MQ=255)

                                                                                                                                                                                                                           aaCA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGTTGCAGTTTTtgct             >  2:519849/1‑238 (MQ=255)

                                                                                                                                                                                                                                ccccGACCCCCAGCCCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTCCCGATCCTGCCGATACGCCCGAGGATCACCGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGTTGCAGTTTTTGCTGCGCa        <  1:201510/236‑1 (MQ=255)

                                                                                                                                                                                                                                  aCGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGTTGCAGTTTTTGCTGCGCa        <  1:194959/237‑1 (MQ=255)

                                                                                                                                                                                                                                      tCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATa                                                  >  2:1067912/1‑191 (MQ=255)

                                                                                                                                                                                                                                      tCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATa                                                  <  1:1067912/191‑1 (MQ=255)

                                                                                                                                                                                                                                      tCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAaca                                              >  1:464862/1‑195 (MQ=255)

                                                                                                                                                                                                                                      tCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGTTGCAGTTTTTGCTGCGCATTccc   >  2:543733/1‑238 (MQ=255)

TTAGCAAACGCCAGCGCCGGAGGATCGGTCATGGAACCTGCCAGCATCCCGCACATGGTCAGGTAATTCATTTTGGCTAACATCCGCGCCAGAATGCCAACAGTAATCAGCGG‑AACGGCGGTGATCAGGGCACCATAACCAAT‑CCAGCTTAGCCCTTCGCCATTGACCAGGGTATTC‑ACAAAATCCCCACCAGATTTCAGACCAACGACCGAGAGGAACA‑GCACGATCCCCAGCTCCCGCAGCGCGAGGTTGGCG‑CTTGGCGGCATAAACCAGTACAGCTTGCCGATACTGCCGATACGCCCGAGGATCAACGCCATAATCAGCGGTCCGCCCGCCAGCCCCAGTTTCAACGCGGCCGGGAATCCTGGCACAAAGACGGGAATAGAACCTAACAATACGCCTAGCCCGATGCCAATAAACACTGGCAGCATCTGAACCTGTTGCAGTTTTTGCTGCGCATTCCCC  >  NC_000913/3864732‑3865196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`