New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3263130 =138 (1.070)3 (0.020) 3/406 NT 2.4% coding (529/1209 nt) tdcD propionate kinase/acetate kinase C, anaerobic
?NC_000913 3263184 = 113 (0.920)coding (475/1209 nt) tdcD propionate kinase/acetate kinase C, anaerobic
Rejected: Frequency below/above cutoff threshold.

GTCCGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAATCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3263371‑3263130
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttccacggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCC  >  NC_000913/3263184‑3263419
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
GTCCGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAATCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACG                                                                                                                                                                                                                                                 >  2:371781/1‑239
GTCCGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAATCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACG                                                                                                                                                                                                                                                 >  1:693071/1‑239
GTCCGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAATCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACG                                                                                                                                                                                                                                                 >  1:111148/1‑239
GTCCGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAATCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACG                                                                                                                                                                                                                                                 >  1:972970/1‑239
 TCCGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAATCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACG                                                                                                                                                                                                                                                 >  2:1133721/1‑238
   CGCCGTTATTACCGATGAAGTCATTGATAATATGCGTCGGGTTTCTCCAGTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTGTTGAATCGGCGAAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCCCCAGACGATGGCTCTGGAAGCTTATTTATACGCCCTGCCGTTGAAATATTATGAAGAGTTAGGTGTACTCCGTTATGGTTTCCACGGCA                                                                                                                                                                                                                                              <  2:1355119/239‑1
   CGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGCCTCGGCGCCGCAATTATTTCCGGGCGTCACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTCCGCCGTTCTGGTTTCCCCGGt                                                                                                                                                                                                                                               >  1:1156378/1‑237
                                   TCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAAGCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGT                                                                                                                                                                                                               <  1:914859/239‑1
                                                          CCCCTGCATAATTACGCCAATTTAAGTGGTATTGAAGCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATAC                                                                                                                                                                                       <  1:932245/239‑1
                                                             cttcttatttgCGCCAATTTAAGTGGTATTGAATCGACGCAGCAATTATTTCCGGTCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGC                                                                                                                                                                                    <  2:111148/228‑1
                                                                                                                                                                                                                                         TTCCACTGCAGGCCGTATAAATAAGCTACCGGAGCCGTCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCCCTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAAT         <  2:237257/239‑1
                                                                                                                                                                                                                                         TTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCCCCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCTCTTAAATTGGCGTAA                                                                                                                       <  2:733143/129‑1
                                                                                                                                                                                                                                         TTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAAT         <  2:820882/239‑1
                                                                                                                                                                                                                                         TTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAAT         >  1:559362/1‑239
                                                                                                                                                                                                                                         TTCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAA                                                                                                                       >  1:733143/1‑129
                                                                                                                                                                                                                                         TTCCACGGCAGGCCGTATAAATAAGCTTCCGCAGCCATCGTCCGGCGGAAACTGGTATCAAATACCGCCAACTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATCGGCGTACTTACTCAGGGGTGCCAGTGGTGAAACGCGACGGATATTATCAATGACTTCATCGGCAATCATGGCGGACTCGCTAAAAATACACCGGCCTTCCGCGATGCGTTGGCatag         >  2:1424075/1‑235
                                                                                                                                                                                                                                          TCCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATT        <  1:1445565/239‑1
                                                                                                                                                                                                                                           CCACGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTA       <  1:1151779/239‑1
                                                                                                                                                                                                                                              CGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAG     >  1:237257/1‑238
                                                                                                                                                                                                                                              CGGCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAG     >  1:1156437/1‑238
                                                                                                                                                                                                                                                GCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCC  >  1:191806/1‑239
                                                                                                                                                                                                                                                GCAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGGGGCCAATTAAGGCC  >  1:1286179/1‑239
                                                                                                                                                                                                                                                 CAGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGGGGCCAATTAAGGCC  >  1:258420/1‑238
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
GTCCGCCATTATTACCGATGAAGTCATTGATAATATCCGTCGCGTTTCTCCACTGGCACCCCTGCATAATTACGCCAATTTAAGTGGTATTGAATCGGCGCAGCAATTATTTCCGGGCGTAACTCAGGTGGCGGTATTTGATACCAGTTTCCACCAGACGATGGCTCCGGAAGCTTATTTATACGGCCTGCCGTGGAAATATTATGAAGAGTTAGGTGTACGCCGTTATGGTTTCCACGGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3263371‑3263130
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tttccacggcaGGCCGTATAAATAAGCTTCCGGAGCCATCGTCTGGTGGAAACTGGTATCAAATACCGCCACCTGAGTTACGCCCGGAAATAATTGCTGCGCCGATTCAATACCACTTAAATTGGCGTAATTATGCAGGGGTGCCAGTGGAGAAACGCGACGGATATTATCAATGACTTCATCGGTAATAATGGCGGACTCGGTAAAAATACTGCCGCCGTGAGCGATGCGGTGGCCAATTAAGGCC  >  NC_000913/3263184‑3263419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.