| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 337,463 | A→G | 20.4% | E229G (GAG→GGG) | yahF → | putative NAD(P)‑binding succinyl‑CoA synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 337,463 | 0 | A | G | 20.4% | 120.4 / 4.4 | 61 | E229G (GAG→GGG) | yahF | putative NAD(P)‑binding succinyl‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base A (13/34); new base G (11/2); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.24e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGC > NC_000913/337233‑337691 | aCTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCCTAGGGGGAAAATcg > 1:607885/1‑239 (MQ=255) cTGGCGCTCAAGCAACTGGCGCACGAAAAAGGGCGGCTGATGCTGGGGTCAGACTGTGGCACGGCGATTATAAACGGCGCGGAGCTCTGTGTTGGGAGCGCCGTGCGTCGCGGGAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTGGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCTGGCGCGACCTGAGCGAGAAAATcgg < 2:601882/239‑1 (MQ=255) cTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATcgg < 1:706083/239‑1 (MQ=255) ggCGCTCAAGCAACTGGCCCACGAAAAAGGGTTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGTCACCGGCGGGCGCGACCTGAGCGAGAAAATcggcg < 1:430548/239‑1 (MQ=255) gcgcTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGGACTGAGCGGGAAAATcggcgg > 1:496164/1‑239 (MQ=255) gcgcTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCCGAGCGAGAAAATcggcg > 1:932136/1‑238 (MQ=255) aaGCAACTGGCCCACGAAAAAGGTGTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAGCGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCGCCGGCGGGCGCGACCTGAGCGTGAAAATCGGCGGCCtga < 2:818366/238‑1 (MQ=255) aGGGGTGCTGGTGATGGGGCTCGGCTGTGGCACGGCGAGTATCAACGGCGGGGCGGTCTGTTTTGGTAACGCCGGGGGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGa < 2:359812/239‑1 (MQ=255) ctgctgATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGGAAACCAGCGGCCGGAGGATGCTCgccccgctccggggccc > 2:1251312/1‑223 (MQ=255) tgctgATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGGGCGAGAAAATCGGCGGCCTGATGGTGCTCG‑ACGCCATCGGGG‑TGCTg > 1:1417183/1‑239 (MQ=255) ctgATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGa > 1:1193423/1‑239 (MQ=255) ggCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCGACCTGAGCGGGAAAATCGGCGGCCTGATGATGATCG‑ACGCCATCGGGG‑TGCTGGACAACGATca > 1:861437/1‑237 (MQ=255) ggCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGGGAAAATCGGCGGCCTGGTGATGCCCG‑GCGCCACCGGGG‑TGCTGGGAAACGATcc > 1:790351/1‑238 (MQ=255) ggCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCCTCCGGG‑TGCTGGGAAAAGATcc > 1:346631/1‑238 (MQ=255) gCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGGAAATATGCGGCCCGCTGTATATCC‑CCGCCATCGGGG‑TGCTTGAAAACGaaacg > 1:1061637/1‑234 (MQ=255) gCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCg < 1:1101725/238‑1 (MQ=255) cAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGGGAAAATCGGCGGCCTGAATATGCTCG‑ACGCCATCGGGA‑GGCTGGAAAACGAACCGCaa > 1:1220640/1‑239 (MQ=255) cAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCa < 1:1417132/238‑1 (MQ=255) tgtgGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGa < 1:1386036/239‑1 (MQ=255) tgtgGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGa < 1:777332/239‑1 (MQ=255) tgGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGAAGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGAGCCGCAAACTGaaa > 1:1233178/1‑239 (MQ=255) gCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAAt < 1:1133111/238‑1 (MQ=255) aCGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTAAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCa < 1:741875/238‑1 (MQ=255) cGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGAACTGAGCGAGGAAATCGGCGGCCTGATGATGCTCG‑GCGCCCTCCGGG‑TGCTGGGAAAAGAACCCCAAACTGAAATCAtt > 1:241868/1‑239 (MQ=255) ggCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTg < 1:1380933/239‑1 (MQ=255) ttgtaaaCCGCGGGGCGCTGTGATCTGGTAACGCCGTGAGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCAGGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGAGCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATTCGAAAACGGAAATCATTGCGC‑t < 2:363915/233‑1 (MQ=255) ttgtCATCGGCGGGGTGCTCTGTTTTGGTAACACCGTGCGTCGCGGCAACTTCGGTATTGTTGGCGCATCCGGCGCCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAGCTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑t < 2:401199/235‑1 (MQ=255) gtATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑t < 2:416194/237‑1 (MQ=255) cgcggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCGGAGCGGGAAAATCGGCGGGCCGGTGATGCTCG‑GCGCCACCCGGA‑TGCTGGAAAACGAACCGCAAACTGAAATCCTTGTGC‑TTTT‑TTCCcaa > 2:683822/1‑238 (MQ=255) cggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGG‑AGCTGGAAAAAGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCaaaac > 1:200229/1‑236 (MQ=255) cggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCCACCTGAGCGGGAAAAACGGCGGCCTGGTGGTGCTCG‑ACGCCCTCCGGA‑GGCTGGAAAACGGTCCGCAAACTTAAATCATTGCGC‑TTTT‑TTCCAAAcc > 2:1268711/1‑238 (MQ=255) ggcgcTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGGGAAAATCCGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACAATCCGCAAAATGCAATCATTGCGC‑TTAT‑CTCCCAAccc > 1:6517/1‑237 (MQ=255) ctctctGTTTTGGTAACGCCGTGCGGTGCGGCAACATCGGTATTGTTGGTGAATCCGGCCCCGGCAGTCAGGAGTTGAGCGGCCGCATTCAGGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGAGCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACcgcc < 2:181388/236‑1 (MQ=255) ttGGGAACGCCGTGCGTCGCGGCACCATCGTTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGcctgcgcctgcg < 2:1324106/239‑1 (MQ=255) tgggTACCGCCGGGCGTCGCGGCAACAGCGGTATTGTTGGCGCGTCCGGCGCGGGCAGTCAGGAGTTGAGCGTCCGCATTCAGGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGcctgcgcctgcg < 2:1424300/237‑1 (MQ=255) cGTGCGTCGCGGCAACGTCGGTATTGTTGGCGCAGCCGGCAGCGGAGATCAGGATTTGAGCGGCCGCATTCATGAATTTGGGGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGAGTCCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGG‑TGCGGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCa < 2:320166/239‑1 (MQ=255) cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCa < 2:905106/239‑1 (MQ=255) cGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTAATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCa < 1:996371/239‑1 (MQ=255) cGTGCGCTGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCa < 2:27070/239‑1 (MQ=255) gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCaaa < 2:1193423/238‑1 (MQ=255) gCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGAACCGGGCGAGAAAATCGGCGGCCTGCTGATGGTCG‑ACGCCACCGGGG‑TGCTGGAAAACCGTACGCAAACTGGAATCATTGCGC‑CTAT‑TTCCCAAACGCCCGCGCCTGCGGTGGCCCGCCAAg > 1:1133818/1‑239 (MQ=255) aTTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGGGACCCGAGCGAGGAAAAAGGCGGGCTGATGATGATCT‑GCGCCCATGGGA‑TGCTGGAAAACACATCGCAAAATTAAATCATTGCGG‑TTATATTCCAAACC‑CCGCCTGCTCCGGTGGCCCGCAACGTGCT‑TGacagtccgccca > 2:238888/1‑227 (MQ=255) gttgGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAAACGGCGGCCTGGTGATGCTCG‑ACGCCCTCGGGG‑TGCTGGAAAACGATCCGCAAAATGAAAAAAATGTCG‑TTAT‑CTCCCAAACCCCCGCCCCTGCGGCGGGCCGCAAAAGGCC‑GGAACgggaggcggccc > 2:535715/1‑227 (MQ=255) ggCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGGGCGACCCGGGGGGGGAAATCCGCGGGCTGGTGGTGCTCG‑CCCCCCTCGGGA‑GGGTGGGAAAAAATCCGCAAAACGAAATACATGCGCTTTTT‑CCCCAAACCCCCTG‑GCCTCCGGCGGGCCCCGAAGTGGTGGGAACGCGCGCGCCCCtcc > 2:293191/1‑236 (MQ=255) ggCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCTTTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCg < 1:1335747/239‑1 (MQ=255) tCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTTCGC‑TTAT‑CTCCAAACCCCCTGCGCCTGCGGTGGCCCGCAAAATGCT‑GGAACGTGCGCGCGCCCGCCGCAAGcc > 1:207543/1‑239 (MQ=255) gaggaggTGGGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTg < 1:1101389/235‑1 (MQ=255) aGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAGCTGATTGGCACCGGCGGGCGCGACCTGGGCGAGAAAAGCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCa < 1:443025/186‑1 (MQ=255) aGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCCAACAGCGCGGGGAAATTCGGGGGCTGATTATGCCAT‑GCCCCACCGGGA‑TTCTTGAAAACGCAGCGCCAACTGAAAATCTTTCGC‑GTAT‑CACCCAACCGCCCGCGCCTGCGGCGGCCCCTCAATTGCT‑GGAACGCGCGCGCGCCTCCCCCCAGCCGGGCGACGACTGCGTCCTcctcgt > 2:172497/1‑234 (MQ=255) tCCGCATTCCTGAATTTGGCGGCCGCGTTTCGCAACTGATTGGCACCGGCGGTCGCGACCTTCACGGGGAACGATGCCGCCCGAGGATTATCG‑GCCCCAACGGGACTGCT‑GCCAACCACCCACAAACAGGAAACATCTCGC‑CTAT‑CCTCACACCCCCCGCGCCTTCGGTGCGCCGCAAAGCGCT‑TGGCAGTCCGCCCGCCCTCCGCCAGCCCGGGGGTGTCCGCCTCCTCGATCggtgc > 2:342869/1‑235 (MQ=255) cGCATCCAGTAATTTGCGTGCGGCTTTTCGCAACTATGTGTGACCGGCGGGCTCGCCCTGAGCGAAAAAATCGGCGGCCTAATGATGCTCG‑ACGCCATCGGGA‑TCCTGGAAACCGATCCGAAAACTGAAACCATGGCCC‑TTAT‑CTCCAACCCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGa < 2:607885/239‑1 (MQ=255) gagaaaTTGGGCGGCGGCGTTTCGCAACTGATGGGCCCCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGAGGAGGCTCG‑GCGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CCCCAACCCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGcc < 2:346631/235‑1 (MQ=255) cATGAATTTGGGGGGGGCGTTTCGCACCTGATTGGCCCCGGGGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGACCCTCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGcc < 2:200229/239‑1 (MQ=255) gggcggcgTTGCGAACGGGATGGGCCCGGGCGGGGGCGACCGGAGGAAAAAAAGCGGCGGCCTGATGATCCCCG‑ACGCCACCGGGA‑TGCTGAAAAACGATCCGCAAACTGAAACAATGGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGGGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAg < 2:1163522/237‑1 (MQ=255) ggtgccttttCGCATGTGAATGGCGGCGGAGGGCGCGACCTGAGCGGGAAAATCGGCGGCCTGATTGTGCTCG‑CCGCCCTGGGGA‑TGCTGGAAAACGACCCGCAAACAGAAATCATTGCTC‑TTAT‑CTCCAACCCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc < 2:1315960/231‑1 (MQ=255) ggcggcgTTTCCCATCTGAGTGGGCCCGGCGGGCGCGCCTGGGGCGAGAAAATGGGCGGCCTGAGGA‑GCCCGAACGCCATCGGGA‑TGCGGGAAAACGCGCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGGGGCCCGCAAAGTGCT‑GGAACGTGCGGGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGc < 2:1069496/238‑1 (MQ=255) tGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGcc < 1:1268711/239‑1 (MQ=255) ggCACCGGCGGGCGCGACCTGAGCGAGAAAACCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCgg < 1:683822/239‑1 (MQ=255) cACCGGGGGGCGGGCCGTGAGCGGAAAAATCGGCGGCCTGATGATGCTGG‑ACGCCTCGGGGA‑TGCTGGAAAACGTCCCGCAAACTGAAATCATGGCGC‑TTAT‑CCCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGGGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGc < 2:241868/238‑1 (MQ=255) gcgACCTGAGCGAGAAAAGCGGCGGCCTGATGATGCTCG‑GCGCCATCGGGA‑TGCTGGAAAACGACCCTCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGc < 1:238888/238‑1 (MQ=255) gACCTGAGCGAGAAAACCGGCGGCCTGATGATGCCCG‑ACCCCATCGGGA‑TGCTGGAAAACGATCCGAAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAgc < 1:535715/239‑1 (MQ=255) | ACTGGCGCTCAAGCAACTGGCCCACGAAAAAGGGCTGCTGATGATGGGGCCAGACTGTGGCACGGCGATTATCAACGGCGCGGCGCTCTGTTTTGGTAACGCCGTGCGTCGCGGCAACATCGGTATTGTTGGCGCATCCGGCACCGGCAGTCAGGAGTTGAGCGTCCGCATTCATGAATTTGGCGGCGGCGTTTCGCAACTGATTGGCACCGGCGGGCGCGACCTGAGCGAGAAAATCGGCGGCCTGATGATGCTCG‑ACGCCATCGGGA‑TGCTGGAAAACGATCCGCAAACTGAAATCATTGCGC‑TTAT‑CTCCAAACCGCCTGCGCCTGCGGTGGCCCGCAAAGTGCT‑GGAACGTGCGCGCGCCTGCCGCAAGCCGGTGGTCGTCTGCTTCCTCGATCGTGGCGAAACGCCAGTGGATGAGCAGGGGCTACAGTTTGCCCGCGGCACCAAAGAGGCAGC > NC_000913/337233‑337691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |