Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,421,184 A→C 17.2% T402P (ACC→CCC)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,421,1840AC17.2% 177.7 / 6.0 92T402P (ACC→CCC) ulaAL‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base A (45/23);  new base C (2/17);  total (47/45)
Fisher's exact test for biased strand distribution p-value = 1.55e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGG  >  NC_000913/4420948‑4421412
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
cGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAc                                                                                                                                                                                                                                        <  1:1073261/238‑1 (MQ=255)
cGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCGGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACGTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAc                                                                                                                                                                                                                                        <  2:389388/238‑1 (MQ=255)
 gAACGCCGTGGTCTGGGGCTTTATGTGGGTCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCg                                                                                                                                                                                                                                      <  1:951166/239‑1 (MQ=255)
   aCGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCgg                                                                                                                                                                                                                                     <  2:1442036/238‑1 (MQ=255)
     gCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTAt                                                                                                                                                                                                                                  <  2:510571/239‑1 (MQ=255)
            tCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                                                                                                                                            <  1:1166344/238‑1 (MQ=255)
                     ttATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGCCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGGTTTTTCTGGTGATGGGGATGGTCGAAATCTTTGGTTGCGTCTGGGTGGTGAAACTCCACGGTATGAGTGCCTGGATggtc                                                                                                                                                                                                                 >  2:950120/1‑237 (MQ=255)
                      tATGTGGGGCACCCTCGGTCAGCTGGTTGCGGTTGCCATCATGGTAGCCTGCGGCTCCTCGATCCTGATTACTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGTCTCGTTCGCTAACCACTTCGACTGCTGGCGGGCGGTGCTGAAGCTTTGTGTGTGGATGGGGATGCTACAAATACTTGGATGGGTCTGGGTGGGGACAATCACCGGTATGAGCGccgtgctgggtg                                                                                                                                                                                                                 >  2:432246/1‑229 (MQ=255)
                           ggggCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCg                                                                                                                                                                                                           <  1:606648/239‑1 (MQ=255)
                            gggCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCgg                                                                                                                                                                                                          >  1:127794/1‑239 (MQ=255)
                                  cATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCTAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGCCTgg                                                                                                                                                                                                     >  1:27933/1‑238 (MQ=255)
                                   aTCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTc                                                                                                                                                                                                   <  1:1276995/239‑1 (MQ=255)
                                       gTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGTATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                                                                                                                                            >  1:1201428/1‑211 (MQ=255)
                                       gTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGTATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGc                                                                                                                                                                                                                            <  2:1201428/211‑1 (MQ=255)
                                       gTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAGTCTTTGGTTGCGTCTGGGCGGTGAAATTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTGGAtt                                                                                                                                                                                              >  1:2262/1‑239 (MQ=255)
                                                      ttGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCCGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgatg                                                                                                                                                                               >  1:1362320/1‑239 (MQ=255)
                                                         gCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGGTTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCgatgat                                                                                                                                                                             >  1:1128544/1‑238 (MQ=255)
                                                               tGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGGCGATTCTGGCACCGCCGATGGTGCAAg                                                                                                                                                                        >  2:514913/1‑237 (MQ=255)
                                                                  tCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGGTCGCTAACCACTTCGGCGGATGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGGTGGTCGAAATCTTTGGTTGCGTCTGGGCGGTGACACTCACCGGTATGGGTGCCAGGGTTGGCCTGGCGGACTGGCTGGTTCTGGCACAGCCGCTGATGCAAGGct                                                                                                                                                                     >  2:1194661/1‑237 (MQ=255)
                                                                         cGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTGACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGGAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGGCTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTtctc                                                                                                                                                              >  2:1288639/1‑237 (MQ=255)
                                                                               ctggacctttttttttCGTGTCTTTACCCCTATTTTGCTCCCTAACGCCGCCATGGGCTGGTTCGCTACCCGCTGCGGCGGCGGGGGTGGGGCGCGGAAGTTTTGTCGGGGGAGGGGGATGATGGAAATCTTTGGTGGCGTCTGGGCGGTGAAACGCACCGGTATGAGTGCCTGGATGGGCATGGCGGCCTGGCCGATTCTGGCACCGCCTATGATGCAAGGCTTCTTCTCCATCGGTa                                                                                                                                                     <  2:1335390/225‑1 (MQ=255)
                                                                                tcgtcctttgtttttCCTGGTTTTACCCCGATTTTCTTCTCTACCGCCACCAGCGGCGTGTTCGTAGACCTCTGCGGCGGGGGGCGTGGGGCGTTGAGGTTTTGTCTGGTGGTGGGGATGATCGAAATCTTTGGTTGCGTCGGGGGGGTGAACCTCCCCGGTATGGGTGCGTGGATGGGCATGGCGGCTTGTTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTAt                                                                                                                                                    <  2:408004/226‑1 (MQ=255)
                                                                                tccaTCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATAGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTa                                                                                                                                                     >  1:94118/4‑238 (MQ=255)
                                                                                             ttCCTGGCTTTATCCCGATTTTCTTCTCTAACGCCACCCTCGGCGTGTTCGCTACCCTCTCCGGCGGCGGGCGTGGGGCGCTGAAGTTTTGTGTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAACCTCCCCGGTATGAGTGCCTGGATGGGCATGGCGGTCTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGcc                                                                                                                                       <  1:152190/239‑1 (MQ=255)
                                                                                              tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCg                                                                                                                                      >  1:841934/1‑239 (MQ=255)
                                                                                              tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCg                                                                                                                                      >  1:1103176/1‑239 (MQ=255)
                                                                                                   gCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGCCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCCGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGCATCAAGGCGTCTTATCCATCGGGATCGCCGTTCTGGCCGtcatc                                                                                                                                 >  2:273234/1‑239 (MQ=255)
                                                                                                     tttACCCCGAGTTTCTTCTCTACCCCCACCATCGGCGTGTCCGTTACCCCCTGCGGCGGCGGGCGGGCGGCGTTGAAGATTTGTTGGGTGATGGGGATGATCGAATTTTTTTTTTGCGTCTGGGCGGCGAAACTCGCCGGTAGGAGTGCCTGGAGGGTCATGGCGGTCTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTcatcat                                                                                                                               <  2:858204/239‑1 (MQ=255)
                                                                                                     tgtcgccgtgttgtctgctATAACGCGACCATGGGCGTGTGCGGTAACGACTCGGAGGGCTGGTGGGGGGCGCTTAGTGTGTGTCTGGATGTGGGGACGAGCGAAATCTTTGGTGGCGTAGGGGGGTTGAAACTCGCGGGTAGGGGTGTGTGGATGGGCTTGGCGGCTTGGTCGTTTCGCGCTCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTcatcat                                                                                                                               <  2:299349/226‑1 (MQ=255)
                                                                                                       tATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGgcacacttg                                                                                                                             >  2:1165399/1‑233 (MQ=255)
                                                                                                        aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGt                                                                                                                            >  1:180850/1‑239 (MQ=255)
                                                                                                        aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGt                                                                                                                            >  1:1047527/1‑239 (MQ=255)
                                                                                                             gATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATg                                                                                                                                                                                                              <  1:539453/154‑1 (MQ=255)
                                                                                                             gATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATg                                                                                                                                                                                                              >  2:539453/1‑154 (MQ=255)
                                                                                                             gATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGCGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGCTGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAAtt                                                                                                                        >  1:425737/1‑238 (MQ=255)
                                                                                                              aTGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGc                                                                                                                      >  1:634121/1‑239 (MQ=255)
                                                                                                               ttgtctcccctACCGCCACCATCGGCTGTTCCTCTAACCTGGCGGGCGGTTGGCGTGGGGCGCTAAGGTTTTGTTTGGTGTGGGGGATGATCTAATTTTTTGGTGCGTTGGGGGCGTTGAAACCCCCCGGTATGGGTGCCTGGATGGGCTGGGTGGACTGGTCGATTTGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                     <  2:993258/230‑1 (MQ=255)
                                                                                                               tgtgttcctcaAACCCCACTAGCGGCGTTTTCGCTCACCTCTTCGGCGGTGGGCTGGCGGCGTTGAAGTTTTGTCTGGGGATGGGGAGGATCAAAATTTTTGGTTGCGTCGGGGCGGTGAACCTCCCCGGTATGAGTGCCTGGATGGGCTTGGCGGACTGGTCTATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                     <  2:407825/232‑1 (MQ=255)
                                                                                                               tgtcgtcttctacgccctccaggggCGTGTTCGTTAACCCCTCCGGGGGCTGGGGGGCGGCGCGGAAGTTTTGTCGGGGGGTGGGGATGATGGAAATCTTTGGTTGCGTTGGGGCGGTGAAACCCCCCGGTATGAGTGCCGGGATGGGCTTGGCGGATTGGTCTATTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGc                                                                                                                      <  2:251511/215‑1 (MQ=255)
                                                                                                                tttcttcACTACCTCCACGATGGGGGGGTTCGCTAACCGCTGCGCCGGCGGGCGTGCGGCGCTGAAGGTTTGTCGGGGGATGGGGATGATAGAATTCTTTGGTTGCGTCGGGGGGGTAAAACTCCCCGGTATGAGGCCGTGGAGGGGCATGGCGGCTTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                     <  2:1025157/237‑1 (MQ=255)
                                                                                                                gtttttctccacgcccccactgggCGTTTTCGCTACCCGTTTGGGGGGGTGGCGGGCGGCTCTGAAGTTTTGTTGGGGGATGGGGATGATCAAATTCTTTGGTGGCGTCGGGGGGGTGAAACTCACCGTTATGAGGGCTGGGAGGGGCATGGCGGATTGTTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCAc                                                                                                                    <  2:1131958/217‑1 (MQ=255)
                                                                                                                gTTCTTCTCTAACCCCCCCAGGGGATTTTGCGCAAACCCCTTCGGCGGTTGGGGGGCGGCGTTGAAGATTTGTCGGGTGAGGGGGATGATAAAAACTTTTGGTTGCGTCTGGGGGGTAAACCTCCCCGTTAGGAGTGCTTGGTTGGGCTGGCGGGATTGTCGTTTTCTGTCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                     <  2:380172/238‑1 (MQ=255)
                                                                                                                   cttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTg                                                                                                                  >  1:955380/1‑238 (MQ=255)
                                                                                                                   cttctCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCCGCTGGCCTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATTGAAATCTTTGGTTGCGTCTGGGCGGTGTAACTCCCCGGTATGAGAGCCTGGGTGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTg                                                                                                                  >  1:661189/1‑238 (MQ=255)
                                                                                                                         tAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGGCTGGTCGATTCTGGCACCGCCCATGATGCCAGGCTTCTTCTCCACCGGTCTCGCCTTTATGGCCGTCATCATTGTAATTGCCCCGGCTtata                                                                                                           >  2:495076/1‑239 (MQ=255)
                                                                                                                         tAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGACGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTtata                                                                                                           >  1:674678/1‑239 (MQ=255)
                                                                                                                           aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTTGCGTGCGGCGCTGAAGATTTGTCTTGTGATAGGGATGATCGAAATCTTTGGTTGCGTCTAGGCGGGGGAACTCACCGGTATGAGTGCCTGGTTGGGCATGGAGGACTGGTCGATTCAAGCACCGCCGATAATGCAAGGCTTCTTCTCCCTCGGATTCTCCTTTATGGCCTTCGTTCTTTGAATTGCACTCGCttgaatg                                                                                                         >  2:250479/1‑234 (MQ=255)
                                                                                                                           aCGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATg                                                                                                         >  1:210955/1‑239 (MQ=255)
                                                                                                                                accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGCCTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGGCATCATTGTAATTGCACTGTCTTATTTGttctt                                                                                                    >  2:658104/1‑239 (MQ=255)
                                                                                                                                accaTCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGttct                                                                                                     >  1:530638/1‑238 (MQ=255)
                                                                                                                                            ttCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGTTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGGATGAGTGCCTGGGTGGGCATGGCGGGCTGGTCGATTCTTGCCCCGCCGGTGATGCAAGGCCTCTTCTCCATCGGTATCGCCTTTATGGCCGTCACCCTTGTAATTTCCCTGGCTTATTTTTTCTTCGCTTGCcgcgc                                                                                        >  2:739527/1‑239 (MQ=255)
                                                                                                                                            ttCCCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTTTCTGGTGATGGGGATGATCGGAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTTCCTGGGTGGGCCTGGCGGGCTGGTCGATTCTTGCCCCGCCGCTGATTCAAGGCTTCTTCTCCAACGGGATCCCCTTTTTGGCCGGCCTCATTGTCATTTCCCTGGGCTTTTTGttctttcgttgcccgg                                                                                         >  2:1105453/1‑227 (MQ=255)
                                                                                                                                                   accatcTTGGGGGGGTGGCGTGGGGCGTGAAGGTTTTGTGTGGTGGTGGGGATAATCAAATTTTTTGGTGGGGTCTGGGGGGTAAACCCCCCCGTTAGGAGTGCCTGGTGGGGCGTGGCGGAGTGGTCGATTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcg                                                                                  <  2:27933/234‑1 (MQ=255)
                                                                                                                                                   aaCCACTGCGGCGGCTGGCGTGCGGCGTGGAAGATTTGTCTGGTGATGGGGATGATAGAAATCTTTGGTTGCGTCGGGGCGGTGAAACTCACCGGTATGAGTGCCGGGATGGGCAGGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcg                                                                                  <  1:948820/239‑1 (MQ=255)
                                                                                                                                                    acctcgtcgtgggcgggcgggcgGCGTTGAGGATTTGGCTGGTGAGGGGGATGTCGAGAATCTGGGGTTGCGTCTGGGCGGTAAACCTCGCCGGAGTGAGGTCGTGGAGGGGCATGGCGGAGTGTGCTATCTTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcgc                                                                                 <  2:406506/228‑1 (MQ=255)
                                                                                                                                                       aCTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGAAGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCa                                                                              >  1:435643/1‑239 (MQ=255)
                                                                                                                                                            ggcggcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaaga                                                                         >  1:328754/1‑239 (MQ=255)
                                                                                                                                                                gcTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGAt                                                                     >  1:763887/1‑239 (MQ=255)
                                                                                                                                                                            cgtggAAGTTTTGTTGGGGGAGGGGGATGTTGAAATTTTTTGTTTGGGTTGGGGGGGTAAACCCCACGGTTGTGGGGGCGTGGAGGGGAGGGGCGTAGTGGGCGTTTGGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaaca                                                          <  2:1157736/234‑1 (MQ=255)
                                                                                                                                                                            cggtGAGGATTTGTCGGGTGATGGGCGTTATCGAATTCTTCGGTTGATTAGGGGCGGTGAACCTCACCGGTACGAGTCCTGGCGTGGGCATGGCGGCGTGGTCGTTTCGGGCGCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGCAGAAGATGCAGAAAaaca                                                          <  2:690293/235‑1 (MQ=255)
                                                                                                                                                                             ggtagaggATTTGGCGGGGGAGGGGGAGGATCAAAATTTTGGGTGGCGTCGGGGGGGTGAAACTCCCCGGTATGAGTGCCGGGAGGGGGGGGGCGGATGGGTCGATTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaaca                                                          <  1:1205967/231‑1 (MQ=255)
                                                                                                                                                                                 aaGTTTTGTTGGGGGAGGGGGATGATCAAATTTTTGGGTGGGTTTGGGGCGTTAAACCTCGCCGGTAGGGGTGCCGGGATGGGCAGGGCGGACTGGTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTgg                                                     <  2:137582/238‑1 (MQ=255)
                                                                                                                                                                                        tGTCTGGGGAGGGGGAGGAGCGAAATCTTGGGTTGCGTCTGGGGGGTGAAACTCCCCGGTATGAGTGCGTGGAGGGGCATGGCGGACTGGTCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACa                                              <  1:457578/239‑1 (MQ=255)
                                                                                                                                                                                          ttggggggtGGGGTTGAGCAAAATTTTTGGTTGGGGCGGGGGGGGGAACCTCCCGGGTATGAGGGCGTGGATGGGCATGGGGGTCTGGTCGATTTGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                            <  2:722293/231‑1 (MQ=255)
                                                                                                                                                                                           tgtggggtGGGAAAGATAGAAGTTTGTGGTTGCGTGTGGGGGGGGAAACTCGCGGGTATGAGGGCTGGGGTGGGTAGGGCTGGGTGGTCTATTGTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                            <  2:335211/231‑1 (MQ=255)
                                                                                                                                                                                           ggggggtgggggATGAGCAAAGTTTTTGGTTGCTTCGGGGGGTTAAACCTCCCCGGTATGGGGGCTGGGGGGGGGGGGGGGGACTGGTCTATTCGGGCCCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                            <  2:841934/230‑1 (MQ=255)
                                                                                                                                                                                            tGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAc                                                                                                                                                                                                                                        >  1:713393/1‑52 (MQ=255)
                                                                                                                                                                                            tGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCAc                                                                                                                                                                                                                                        <  2:713393/52‑1 (MQ=255)
                                                                                                                                                                                            tGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaacaac                                                        >  2:169643/1‑225 (MQ=255)
                                                                                                                                                                                            tGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaacaac                                                        <  1:169643/225‑1 (MQ=255)
                                                                                                                                                                                              gTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCt                                          >  2:722135/1‑237 (MQ=255)
                                                                                                                                                                                               tGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGGATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGACGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCt                                       >  2:1416610/1‑239 (MQ=255)
                                                                                                                                                                                                        tgatCGAATTCTTTGGTGGGGTGGGGGTGGTAAAACCCACGGGTAGGGGTGCCTGGGTGGGCAGGGCGGCTTGGTCTATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAgg                               <  2:1100627/238‑1 (MQ=255)
                                                                                                                                                                                                        tgatCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGa                              >  1:1288730/1‑239 (MQ=255)
                                                                                                                                                                                                        agtaccaaattttttGGTTGCGTCTGGGCGTTAAACCCCACCGGTATGAGTGCCGGGATGGGCGTGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAg                                <  1:238117/226‑1 (MQ=255)
                                                                                                                                                                                                         gatCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                             >  1:388572/1‑239 (MQ=255)
                                                                                                                                                                                                           tcaaaaTCTTGGGTTGCGTCTGGGGGGTAAAACCCACGGATATGGGTGCCTGGAGGGGCTTGGCGGACTGGTCGTTTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                           <  1:950120/236‑1 (MQ=255)
                                                                                                                                                                                                           tCGAAATTTTGGGTTGCGTCTGGGGGGTAAAACCCCCCGGTATGAGTGCCTGGAGGGGCATGGGGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                           <  1:514913/239‑1 (MQ=255)
                                                                                                                                                                                                            cGAATTCTGTGGTGTGGTTTGGGCGGTGAAACTCACGTGGATGGGGCCCGGGATGGGCATGGCGGACTGGTCGATTCTGGATCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                           <  1:1082207/238‑1 (MQ=255)
                                                                                                                                                                                                            cGAAATTTTGGTTTGCTTCGGGGGGGTGAAACTCACCGGTATGAGTGCCGGGAGGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                           <  1:1056608/239‑1 (MQ=255)
                                                                                                                                                                                                              gaattgtttGGTTGGGTCGGGGGGGTGAACCTCCCCGGGTTGAGGGCCTGGATGGGTGTGGGGGACTGGTGGTTTCGGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                           <  2:634121/231‑1 (MQ=255)
                                                                                                                                                                                                                aaTCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGa                       >  2:1129694/1‑239 (MQ=255)
                                                                                                                                                                                                                     ttGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATg                  >  1:701954/1‑239 (MQ=255)
                                                                                                                                                                                                                     ttGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATg                  >  1:110383/1‑239 (MQ=255)
                                                                                                                                                                                                                      tGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                             >  1:1334641/1‑227 (MQ=255)
                                                                                                                                                                                                                      tGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATg                  >  1:1230529/1‑238 (MQ=255)
                                                                                                                                                                                                                        tttGCGTCTGGGGGGTAAAACCCCCGGGTATGAGGGCCTGGAGGGGCTTGGCGGATTGTGCGTTTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                <  1:625161/237‑1 (MQ=255)
                                                                                                                                                                                                                        ggtGCGTCGGGGCGGGAAACCGCCCGGGTAGGGGTCGCTGTGTGGGCTGGGCGTCCTGGTCTATTCTGGCCCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                <  2:127794/236‑1 (MQ=255)
                                                                                                                                                                                                                           gCGTCGGGCCGGTAAAACCCACCTGTATGAGTCCCTGTATGGGCTTGGCGGCCTTGTATTTTCGGGATCCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta            <  2:435643/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gTGAAACTCCCCGGTATGAGTGCGTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg  <  1:937599/238‑1 (MQ=255)
                                                                                                                                                                                                                                         tGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg  >  1:1138145/1‑237 (MQ=255)
                                                                                                                                                                                                                                         tGAAACTCACCGGTATGAGTGCCCGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTg   >  1:962922/1‑236 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       
CGAACGCCGTGGTCTGGGGCTTTATGTGGGGCACCATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTTCGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAAGATTTGTCTGGTGATGGGGATGATCGAAATCTTTGGTTGCGTCTGGGCGGTGAAACTCACCGGTATGAGTGCCTGGATGGGCATGGCGGACTGGTCGATTCTGGCACCGCCGATGATGCAAGGCTTCTTCTCCATCGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGG  >  NC_000913/4420948‑4421412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: