Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,421,215	A→C	13.1%	D412A (GAC→GCC)	ulaA →	L‑ascorbate‑specific enzyme IIC permease component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,421,215	0	A	C	13.1%	144.4 / 3.8	91	D412A (GAC→GCC)	ulaA	L‑ascorbate‑specific enzyme IIC permease component of PTS
Reads supporting (aligned to +/- strand):  ref base A (42/26);  new base C (2/11);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 4.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAG  >  NC_000913/4420982‑4421453                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  cATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCTA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGCCTgg                                                                                                                                                                                                                                               >  1:27933/1‑238 (MQ=255)  aTCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTc                                                                                                                                                                                                                                             <  1:1276995/239‑1 (MQ=255)      gTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AGTCTTTGGTTGCG‑TCT‑GGGCGGTGAAATTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTGG‑Att                                                                                                                                                                                                                                        >  1:2262/1‑239 (MQ=255)                     ttGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCCGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCgatg                                                                                                                                                                                                                        >  1:1362320/1‑239 (MQ=255)                        gCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GGTTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCgatgat                                                                                                                                                                                                                      >  1:1128544/1‑238 (MQ=255)                              tGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGGCG‑ATTCTGG‑CACCGCCGATGGTGCAAg                                                                                                                                                                                                                 >  2:514913/1‑237 (MQ=255)                                 tCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGGT‑CGCTAACCACTTCGGCGGATGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGGTGGTCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGACACTCACCGGTATGGGTGCCAGG‑GTTGGCCTGGCGGACTGGCTG‑GTTCTGG‑CACAGCCGCTGATGCAAGGct                                                                                                                                                                                                              >  2:1194661/1‑237 (MQ=255)                                        cGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTGACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGGAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGGCTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTtctc                                                                                                                                                                                                       >  2:1288639/1‑237 (MQ=255)                                              ctggacctttttttttCGTGTCTTTACCCCTATTTTGCTCCCTAACGCCGCCATGGGCTGGTT‑CGCTACCCGCTGCGGCGGCGGGGGTGGGGCGCGGAA‑GTTTTGTCGGGGGAGGGGGATGATGGA‑AATCTTTGGTGGCG‑TCT‑GGGCGGTGAAACGCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGCCTGGCCG‑ATTCTGG‑CACCGCCTATGATGCAAGGCTTCTTCTCCATC‑GGTa                                                                                                                                                                                              <  2:1335390/225‑1 (MQ=255)                                               tcgtcctttgtttttCCTGGTTTTACCCCGATTTTCTTCTCTACCGCCACCAGCGGCGTGTT‑CGTAGACCTCTGCGGCGGGGGGCGTGGGGCGTTGAG‑GTTTTGTCTGGTGGTGGGGATGATCGA‑AATCTTTGGTTGCG‑TCG‑GGGGGGTGAACCTCCCCGGTATGGGTGCGTGG‑ATGGGCATGGCGGCTTGTTCG‑TTTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTAt                                                                                                                                                                                             <  2:408004/226‑1 (MQ=255)                                               tccaTCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATAGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTa                                                                                                                                                                                              >  1:94118/4‑238 (MQ=255)                                                            ttCCTGGCTTTATCCCGATTTTCTTCTCTAACGCCACCCTCGGCGTGTT‑CGCTACCCTCTCCGGCGGCGGGCGTGGGGCGCTGAA‑GTTTTGTGTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAACCTCCCCGGTATGAGTGCCTGG‑ATGGGCATGGCGGTCTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGcc                                                                                                                                                                                <  1:152190/239‑1 (MQ=255)                                                             tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCg                                                                                                                                                                               >  1:841934/1‑239 (MQ=255)                                                             tCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCg                                                                                                                                                                               >  1:1103176/1‑239 (MQ=255)                                                                  gCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGCCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCC‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGCATCAAGGCGTCTTATCCATC‑GGGATCGCCGTTCTGGCCGtcatc                                                                                                                                                                          >  2:273234/1‑239 (MQ=255)                                                                    tttACCCCGAGTTTCTTCTCTACCCCCACCATCGGCGTGTC‑CGTTACCCCCTGCGGCGGCGGGCGGGCGGCGTTGAA‑GATTTGTTGGGTGATGGGGATGATCGA‑ATTTTTTTTTTGCG‑TCT‑GGGCGGCGAAACTCGCCGGTAGGAGTGCCTGG‑AGGGTCATGGCGGTCTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTcatcat                                                                                                                                                                        <  2:858204/239‑1 (MQ=255)                                                                    tgtcgccgtgttgtctgctATAACGCGACCATGGGCGTGTG‑CGGTAACGACTCGGAGGGCTGGTGGGGGGCGCTTAG‑TGTGTGTCTGGATGTGGGGACGAGCGA‑AATCTTTGGTGGCG‑TAG‑GGGGGTTGAAACTCGCGGGTAGGGGTGTGTGG‑ATGGGCTTGGCGGCTTGGTCG‑TTTCGCG‑CTCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTcatcat                                                                                                                                                                        <  2:299349/226‑1 (MQ=255)                                                                      tATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGgcacacttg                                                                                                                                                                      >  2:1165399/1‑233 (MQ=255)                                                                       aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGt                                                                                                                                                                     >  1:180850/1‑239 (MQ=255)                                                                       aTCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGt                                                                                                                                                                     >  1:1047527/1‑239 (MQ=255)                                                                            gATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGCGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGCTGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAAtt                                                                                                                                                                 >  1:425737/1‑238 (MQ=255)                                                                             aTGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGc                                                                                                                                                               >  1:634121/1‑239 (MQ=255)                                                                              ttgtctcccctACCGCCACCATCGGC‑TGTTCCTCTAACCTGGCGGGCGGTTGGCGTGGGGCGCTAAG‑GTTTTGTTTGGTGTGGGGGATGATCTA‑ATTTTTTGG‑TGCGTTGG‑GGGCGTTGAAACCCCCCGGTATGGGTGCCTGG‑ATGGGCTGGGTGGACTGGTCG‑ATTTGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                                                              <  2:993258/230‑1 (MQ=255)                                                                              tgtgttcctcaAACCCCACTAGCGGCGTTTT‑CGCTCACCTCTTCGGCGGTGGGCTGGCGGCGTTGAA‑GTTTTGTCTGGGGATGGGGAGGATCAA‑AATTTTTGGTTGCG‑TCG‑GGGCGGTGAACCTCCCCGGTATGAGTGCCTGG‑ATGGGCTTGGCGGACTGGTCT‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                                                              <  2:407825/232‑1 (MQ=255)                                                                              tgtcgtcttctacgccctccaggggCGTGTT‑CGTTAACCCCTCCGGGGGCTGGGGGGCGGCGCGGAA‑GTTTTGTCGGGGGGTGGGGATGATGGA‑AATCTTTGGTTGCG‑TTG‑GGGCGGTGAAACCCCCCGGTATGAGTGCCGGG‑ATGGGCTTGGCGGATTGGTCT‑ATTCGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGc                                                                                                                                                               <  2:251511/215‑1 (MQ=255)                                                                               tttcttcACTACCTCCACGATGGGGGGGTT‑CGCTAACCGCTGCGCCGGCGGGCGTGCGGCGCTGAA‑GGTTTGTCGGGGGATGGGGATGATAGA‑ATTCTTTGGTTGCG‑TCG‑GGGGGGTAAAACTCCCCGGTATGAGGCCGTGG‑AGGGGCATGGCGGCTTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                                                              <  2:1025157/237‑1 (MQ=255)                                                                               gtttttctccacgcccccactgggCGTTTT‑CGCTACCCGTTTGGGGGGGTGGCGGGCGGCTCTGAA‑GTTTTGTTGGGGGATGGGGATGATCAA‑ATTCTTTGGTGGCG‑TCG‑GGGGGGTGAAACTCACCGTTATGAGGGCTGGG‑AGGGGCATGGCGGATTGTTCG‑TTTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCAc                                                                                                                                                             <  2:1131958/217‑1 (MQ=255)                                                                               gTTCTTCTCTAACCCCCCCAGGGGATTTTG‑CGCAAACCCCTTCGGCGGTTGGGGGGCGGCGTTGAA‑GATTTGTCGGGTGAGGGGGATGATAAA‑AACTTTTGGTTGCG‑TCT‑GGGGGGTAAACCTCCCCGTTAGGAGTGCTTGG‑TTGGGC‑TGGCGGGATTGTCGTTTTCTGT‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCa                                                                                                                                                              <  2:380172/238‑1 (MQ=255)                                                                                  cttctCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTg                                                                                                                                                           >  1:955380/1‑238 (MQ=255)                                                                                  cttctCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCCGCTGGCCTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATTGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGTAACTCCCCGGTATGAGAGCCTGG‑GTGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTg                                                                                                                                                           >  1:661189/1‑238 (MQ=255)                                                                                        tAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGGCTGGTCG‑ATTCTGG‑CACCGCCCATGATGCCAGGCTTCTTCTCCACC‑GGTCTCGCCTTTATGGCCGTCATCATTGTAATTGCCCCGGCTtata                                                                                                                                                    >  2:495076/1‑239 (MQ=255)                                                                                        tAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ACGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTtata                                                                                                                                                    >  1:674678/1‑239 (MQ=255)                                                                                          aCGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTTGCGTGCGGCGCTGAA‑GATTTGTCTTGTGATAGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑AGGCGGGGGAACTCACCGGTATGAGTGCCTGG‑TTGGGCATGGAGGACTGGTCG‑ATTCAAG‑CACCGCCGATAATGCAAGGCTTCTTCTCCCTC‑GGATTCTCCTTTATGGCCTTCGTTCTTTGAATTGCACTCGCttgaatg                                                                                                                                                  >  2:250479/1‑234 (MQ=255)                                                                                          aCGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATg                                                                                                                                                  >  1:210955/1‑239 (MQ=255)                                                                                               accaTCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGCCTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGGCATCATTGTAATTGCACTGTCTTATTTGttctt                                                                                                                                             >  2:658104/1‑239 (MQ=255)                                                                                               accaTCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGttct                                                                                                                                              >  1:530638/1‑238 (MQ=255)                                                                                                           tt‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGTTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGGATGAGTGCCTGG‑GTGGGCATGGCGGGCTGGTCG‑ATTCTTG‑CCCCGCCGGTGATGCAAGGCCTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCACCCTTGTAATTTCCCTGGCTTATTTTTTCTTCGCTTGCcgcgc                                                                                                                                 >  2:739527/1‑239 (MQ=255)                                                                                                           tt‑CCCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTTTCTGGTGATGGGGATGATCGG‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTTCCTGG‑GTGGGCCTGGCGGGCTGGTCG‑ATTCTTG‑CCCCGCCGCTGATTCAAGGCTTCTTCTCCAAC‑GGGATCCCCTTTTTGGCCGGCCTCATTGTCATTTCCCTGGGCTTTTTGttctttcgttgcccgg                                                                                                                                  >  2:1105453/1‑227 (MQ=255)                                                                                                                  accatcTTGGGGGGGTGGCGTGGGGCG‑TGAAGGTTTTGTGTGGTGGTGGGGATAATCAA‑ATTTTTTGGTGGGG‑TCT‑GGGGGGTAAACCCCCCCGTTAGGAGTGCCTGG‑TGGGGCGTGGCGGAGTGGTCG‑ATTCGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcg                                                                                                                           <  2:27933/234‑1 (MQ=255)                                                                                                                  aaCCACTGCGGCGGCTGGCGTGCGGCGTGGAA‑GATTTGTCTGGTGATGGGGATGATAGA‑AATCTTTGGTTGCG‑TCG‑GGGCGGTGAAACTCACCGGTATGAGTGCCGGG‑ATGGGCAGGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcg                                                                                                                           <  1:948820/239‑1 (MQ=255)                                                                                                                   acctcgtcgtgggcgggcgggcgGCGTTGAG‑GATTTGGCTGGTGAGGGGGATG‑TCGAGAATCTGGGGTTGCG‑TCT‑GGGCGGTAAACCTCGCCGGAGTGAGGTCGTGG‑AGGGGCATGGCGGAGTGTGCT‑ATCTTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTgcgc                                                                                                                          <  2:406506/228‑1 (MQ=255)                                                                                                                      aCTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGAAGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCa                                                                                                                       >  1:435643/1‑239 (MQ=255)                                                                                                                           ggcggcTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCagaaga                                                                                                                  >  1:328754/1‑239 (MQ=255)                                                                                                                               gcTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGAt                                                                                                              >  1:763887/1‑239 (MQ=255)                                                                                                                                           cgtggAA‑GTTTTGTTGGGGGAGGGGGATGTTGAA‑ATTTTTTGTTTGGG‑TTG‑GGGGGGTAAACCCCACGGTTGTGGGGGCGTGG‑AGGGGAGGGGCGTAGTGGGCG‑TTTGGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaaca                                                                                                   <  2:1157736/234‑1 (MQ=255)                                                                                                                                           cggtGAG‑GATTTGTCGGGTGATGGGCGTTATCGA‑ATTCTTCGGTTGAT‑TAG‑GGGCGGTGAACCTCACCGGTACGAGT‑CCTGGCGTGGGCATGGCGGCGTGGTCG‑TTTCGGG‑CGCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGCAGAAGATGCAGAAAaaca                                                                                                   <  2:690293/235‑1 (MQ=255)                                                                                                                                            ggtagaggATTTGGCGGGGGAGGGGGAGGATCAA‑AATTTTGGGTGGCG‑TCG‑GGGGGGTGAAACTCCCCGGTATGAGTGCCGGG‑AGGGGGGGGGCGGATGGGTCG‑ATTCGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaaca                                                                                                   <  1:1205967/231‑1 (MQ=255)                                                                                                                                                aa‑GTTTTGTTGGGGGAGGGGGATGATC‑A‑AATTTTTGGGTGGG‑TTTGGGGCGTTAAACCTCGCCGGTAGGGGTGCCGGG‑ATGGGCAGGGCGGACTGGTCG‑TTTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTgg                                                                                              <  2:137582/238‑1 (MQ=255)                                                                                                                                                       tGTCTGGGGAGGGGGAGGAGCGA‑AATCTTGGGTTGCG‑TCT‑GGGGGGTGAAACTCCCCGGTATGAGTGCGTGG‑AGGGGCATGGCGGACTGGTCG‑TTTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACa                                                                                       <  1:457578/239‑1 (MQ=255)                                                                                                                                                         ttggggggtGGGGTTGAGCAA‑AATTTTTGGTTGGG‑GCG‑GGGGGGGGAACCTCCCGGGTATGAGGGCGTGG‑ATGGGCATGGGGGTCTGGTCG‑ATTTGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                                                     <  2:722293/231‑1 (MQ=255)                                                                                                                                                          tgtggggtGGGAAAGATAGA‑AGTTTGTGGTTGCG‑TGT‑GGGGGGGGAAACTCGCGGGTATGAGGGCTGGG‑GTGGGTAGGGCTGGGTGGTCT‑ATTGTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                                                     <  2:335211/231‑1 (MQ=255)                                                                                                                                                          ggggggtgggggATGAGCAA‑AGTTTTTGGTTGCT‑TCG‑GGGGGTTAAACCTCCCCGGTATGGGGGCTGGG‑GGGGGGGGGGGGGACTGGTCT‑ATTCGGG‑CCCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGt                                                                                     <  2:841934/230‑1 (MQ=255)                                                                                                                                                           tGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaacaac                                                                                                 >  2:169643/1‑225 (MQ=255)                                                                                                                                                           tGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAaacaac                                                                                                 <  1:169643/225‑1 (MQ=255)                                                                                                                                                             gTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCt                                                                                   >  2:722135/1‑237 (MQ=255)                                                                                                                                                              tGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGGATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGACGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCt                                                                                >  2:1416610/1‑239 (MQ=255)                                                                                                                                                                       tgatCGA‑ATTCTTTGGTGGGG‑TGG‑GGGTGGTAAAACCCACGGGTAGGGGTGCCTGG‑GTGGGCAGGGCGGCTTGGTCT‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAgg                                                                        <  2:1100627/238‑1 (MQ=255)                                                                                                                                                                       tgatCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGa                                                                       >  1:1288730/1‑239 (MQ=255)                                                                                                                                                                       agtaccaaattttttGGTTGCG‑TCT‑GGGCGTTAAACCCCACCGGTATGAGTGCCGGG‑ATGGGCGTGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAg                                                                         <  1:238117/226‑1 (MQ=255)                                                                                                                                                                        gatCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                                                      >  1:388572/1‑239 (MQ=255)                                                                                                                                                                          tcaa‑aaTCTTGGGTTGCG‑TCT‑GGGGGGTAAAACCCACGGATATGGGTGCCTGG‑AGGGGCTTGGCGGACTGGTCG‑TTTCGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                                                    <  1:950120/236‑1 (MQ=255)                                                                                                                                                                          tCGA‑AATTTTGGGTTGCG‑TCT‑GGGGGGTAAAACCCCCCGGTATGAGTGCCTGG‑AGGGGCATGGGGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                                                    <  1:514913/239‑1 (MQ=255)                                                                                                                                                                           cGA‑ATTCTGTGGTGTGG‑TTT‑GGGCGGTGAAACTCACGTGGATGGGGCCCGGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑ATCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                                                    <  1:1082207/238‑1 (MQ=255)                                                                                                                                                                           cGA‑AATTTTGGTTTGCT‑TCG‑GGGGGGTGAAACTCACCGGTATGAGTGCCGGG‑AGGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATCGGGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                                                    <  1:1056608/239‑1 (MQ=255)                                                                                                                                                                             gaattgtttGGTTGGG‑TCG‑GGGGGGTGAACCTCCCCGGGTTGAGGGCCTGG‑ATGGGTGTGGGGGACTGGTGG‑TTTCGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGtt                                                                    <  2:634121/231‑1 (MQ=255)                                                                                                                                                                               aaTCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGa                                                                >  2:1129694/1‑239 (MQ=255)                                                                                                                                                                                    ttGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATg                                                           >  1:110383/1‑239 (MQ=255)                                                                                                                                                                                    ttGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATg                                                           >  1:701954/1‑239 (MQ=255)                                                                                                                                                                                     tGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAg                                                                      >  1:1334641/1‑227 (MQ=255)                                                                                                                                                                                     tGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATg                                                           >  1:1230529/1‑238 (MQ=255)                                                                                                                                                                                       tttGCG‑TCT‑GGGGGGTAAAACCCCCGGGTATGAGGGCCTGG‑AGGGGCTTGGCGGATTGTGCG‑TTTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                                                         <  1:625161/237‑1 (MQ=255)                                                                                                                                                                                       ggtGCG‑TCG‑GGGCGGGAAACCGCCCGGGTAGGGGTCGCTGT‑GTGGGCTGGGCGTCCTGGTCT‑ATTCTGG‑CCCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGAc                                                         <  2:127794/236‑1 (MQ=255)                                                                                                                                                                                          gCG‑TCG‑GGCCGGTAAAACCCACCTGTATGAGTCCCTGT‑ATGGGCTTGGCGGCCTTGTAT‑TTTCGGG‑ATCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACcgta                                                     <  2:435643/239‑1 (MQ=255)                                                                                                                                                                                                       gtgaaccctcccGGTTTGGGTGCTGGG‑GGGGGGATGGCGGGCTGGTCT‑ATTCTGG‑CGCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg                                           <  2:1290099/228‑1 (MQ=255)                                                                                                                                                                                                       gTGAAACTCCCCGGTATGAGTGCGTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg                                           <  1:937599/238‑1 (MQ=255)                                                                                                                                                                                                        tGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTgg                                           >  1:1138145/1‑237 (MQ=255)                                                                                                                                                                                                        tGAAACTCACCGGTATGAGTGCCCGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTg                                            >  1:962922/1‑236 (MQ=255)                                                                                                                                                                                                             ctcgcgggTGTGGGTGCCGGG‑ATGGGGTGGGCGGCTTGGGTG‑‑TTCTGGCCCCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCtgtgtg                                    <  2:1230529/233‑1 (MQ=255)                                                                                                                                                                                                                  cGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCa                               >  1:1252417/1‑239 (MQ=255)                                                                                                                                                                                                                     aatgggtggttGG‑ATGGGCTTGGCGGATTGGTCG‑TTTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCa                               <  1:250479/226‑1 (MQ=255)                                                                                                                                                                                                                          gTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACaa                       >  2:808323/1‑239 (MQ=255)                                                                                                                                                                                                                           tGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAg                      >  1:790900/1‑239 (MQ=255)                                                                                                                                                                                                                            gCCTGG‑ATGGGCATGGCGGCTGGGCCG‑GTTCTGC‑CGCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGTCAAgg                     <  1:1023435/239‑1 (MQ=255)                                                                                                                                                                                                                             gcTGG‑ATGGGCATGGCGGACTGGTCG‑TTGCGGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAgg                     <  2:790900/237‑1 (MQ=255)                                                                                                                                                                                                                             cgggg‑ATGGGCTTGGCGGACGGTTCT‑ATTCGGC‑CTCCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAgg                     <  2:1047527/235‑1 (MQ=255)                                                                                                                                                                                                                             ccTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGc                    >  2:713555/1‑239 (MQ=255)                                                                                                                                                                                                                              tgtgggtgtggaTGGCGTCCTTGTCG‑TTTGTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAgg                     <  2:226480/227‑1 (MQ=255)                                                                                                                                                                                                                                      ggCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCAt            >  2:1207516/1‑239 (MQ=255)                                                                                                                                                                                                                                      ggCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCAt            >  1:1358789/1‑239 (MQ=255)                                                                                                                                                                                                                                      ggCAGGGCGGACGGGTCT‑ATTCGGC‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCa             <  1:1288639/238‑1 (MQ=255)                                                                                                                                                                                                                                            gCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCAt      >  1:909438/1‑239 (MQ=255)                                                                                                                                                                                                                                                aCTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAg  >  1:760945/1‑239 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                  CATCGGTCAGCTGATTGCGGTTGGCATCCTGGTCGCCTGCGGCTCCTCGATCCTGATTATTCCTGGCTTTATCCCGATGTTCTTCTCTAACGCCACCATCGGCGTGTT‑CGCTAACCACTTCGGCGGCTGGCGTGCGGCGCTGAA‑GATTTGTCTGGTGATGGGGATGATCGA‑AATCTTTGGTTGCG‑TCT‑GGGCGGTGAAACTCACCGGTATGAGTGCCTGG‑ATGGGCATGGCGGACTGGTCG‑ATTCTGG‑CACCGCCGATGATGCAAGGCTTCTTCTCCATC‑GGTATCGCCTTTATGGCCGTCATCATTGTAATTGCACTGGCTTATATGTTCTTCGCTGGCCGCGCGCTGCGCGCAGAAGAAGATGCAGAAAAACAACTGGCAGAACAGTCTGCTTAATAAGGAGTTTTGATTATGACCGTACGTATTCTGGCTGTGTGTGGCAACGGACAAGGCAGTTCCATGATCATGAAG  >  NC_000913/4420982‑4421453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑