Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,465,779 T→C 11.1% I135V (ATC→GTC)  treB ← trehalose‑specific PTS enzyme: IIB and IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,465,7790TC11.1% 257.7 / 5.2 96I135V (ATC→GTC) treBtrehalose‑specific PTS enzyme: IIB and IIC component
Reads supporting (aligned to +/- strand):  ref base T (70/13);  new base C (2/9);  total (72/24)
Fisher's exact test for biased strand distribution p-value = 1.91e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAG  >  NC_000913/4465542‑4466014
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
ccGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATc                                                                                                                                                                                                                                              >  1:383985/1‑239 (MQ=255)
ccGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCCATCAACCACAGAAAATCGTAGATCGTTTGCATGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCCCATGGGCAAATAGCCGAt                                                                                                                                                                                                                                               >  2:595532/1‑238 (MQ=255)
      gctgctGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACAtt                                                                                                                                                                                                                                         >  1:493444/1‑238 (MQ=255)
          ctgcCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCCATCCACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGaa                                                                                                                                                                                                                                    >  2:571222/1‑239 (MQ=255)
            gcCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGTCCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGaaaa                                                                                                                                                                                                                                  <  1:761163/239‑1 (MQ=255)
              ccGAGCAGATAAGCGTTCATCAGCTGTTGAGAAACCAGTGCCACGCCAAGCACGATACCAAGGAGCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAAcc                                                                                                                                                                                                                                <  1:251478/239‑1 (MQ=255)
               cGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCg                                                                                                                                                                                                                               <  1:104519/239‑1 (MQ=255)
               cGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCg                                                                                                                                                                                                                               <  2:437125/239‑1 (MQ=255)
                  gCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCCGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGAACGTTTGCAGGGAAGGGCACATTTGCGCCAGCGTCTGACCCTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAgg                                                                                                                                                                                                                            >  1:579644/1‑239 (MQ=255)
                             gtcagagctgggggAAAACCCAGGCTCACCCCAACAACAATACCAAGCAGCGTCGTGCCGCCGATTTTTTTCCCCGCCGACCAGAAAATCCCGCCCGGAGGGTAGAAGAAGATCCTTTCCCCGACCAACCAAAGAAATTCGTAGATCGTTTGCAGGGAAGGGTACATGTGCCCCAGCGTCTGCCCGTTGCTAATGGCAAAATCGCCGATCAATTTGCGAAACCCGAGTATAACAccgcc                                                                                                                                                                                                               <  2:647217/227‑1 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTTCAGGGAAGGGAACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAACCCGAGGATCAAACCGCCGCt                                                                                                                                                                                                            >  1:201026/1‑239 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCCGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATAACATTACGAAAACCGAGGATCAAACCGCCGCt                                                                                                                                                                                                            >  1:444632/1‑239 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCt                                                                                                                                                                                                            >  2:183771/1‑239 (MQ=255)
                                atcaGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGAAATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGGTCAAACCGCCCCt                                                                                                                                                                                                            >  2:84964/1‑239 (MQ=255)
                                        tgGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAAc                                                                                                                                                                                                     >  2:1350907/1‑238 (MQ=255)
                                        tgGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAAc                                                                                                                                                                                                     >  1:828971/1‑238 (MQ=255)
                                        tgGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAAc                                                                                                                                                                                                     >  1:41061/1‑238 (MQ=255)
                                          gagaAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAAcgcg                                                                                                                                                                                                  >  1:71103/1‑239 (MQ=255)
                                               aCCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGgca                                                                                                                                                                                              >  1:732966/1‑238 (MQ=255)
                                                  aGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACCCGGgcagca                                                                                                                                                                                           >  1:420171/1‑238 (MQ=255)
                                                    tgtCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCCCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACg                                                                                                                                                                                        >  2:1096134/1‑239 (MQ=255)
                                                         cGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCCGCAACGGgaag                                                                                                                                                                                   >  1:90643/1‑239 (MQ=255)
                                                         cGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGgaag                                                                                                                                                                                   >  2:243448/1‑239 (MQ=255)
                                                         cGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCCGTGATCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACAGCCGCTCAACAACTCGGGCAGCAACGGgaag                                                                                                                                                                                   >  2:1009193/1‑239 (MQ=255)
                                                              aGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCTCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGCACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCCAATCAAAGCGGGCAGCAACGGGAagaaga                                                                                                                                                                               >  2:1268313/1‑238 (MQ=255)
                                                                cACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGGACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATc                                                                                                                                                                             >  1:321432/1‑238 (MQ=255)
                                                                    aTACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACcgc                                                                                                                                                                        >  1:1362381/1‑239 (MQ=255)
                                                                         agggAGCGGGGGGCCGCCTATTTTTTCCACGGCTAACAAAAAAAAACCGGCCGGAAGGTAGAAGAAGATCGCTTCACCGATAAACCACAAAAAATCGAAGTTGGTTGGCAGGGAAGGGTACATTTGGCCAAGGGCCGGACCGTTGCTAATGGGAAAACCGCCGATCAAATTGAGAAAACAGAGGATAAACCCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAAt                                                                                                                                                                   <  2:732966/237‑1 (MQ=255)
                                                                          aGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGCTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATg                                                                                                                                                                  >  1:648986/1‑239 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATgagaga                                                                                                                                                             >  1:828327/1‑239 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAAAGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATgagcaa                                                                                                                                                             >  2:1041853/1‑236 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGACCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATgagaga                                                                                                                                                             >  1:993610/1‑239 (MQ=255)
                                                                               cGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGATCAACCCCAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTTCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGCTCACATTTCGAAAACCGAGGATCAAACCGCCGCCACTCAACGCGGGCAGCAAAGGGAAGAAGATAACAGCGAAATgagagc                                                                                                                                                             >  2:1743/1‑238 (MQ=255)
                                                                                ggCGTGCCGCCCATTTTTTTCACCGGTGACCAGCAAAGACCGACCGGCAGGAAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGGAGATCGTTTGCAGGGAAGGGTACATTTACCCCAGCGTCTGACCGTTGCTCATGGGCTAATCGCATATCACATTGCGAAAACCGCGGATCAAACCGCCGCTAATCAACGCGGGCAGAAACGGGAAGAAAAACACCGCGaacagatagc                                                                                                                                                             >  2:337447/1‑230 (MQ=255)
                                                                                ggCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGAAGAAGAAGATTGCTTCAACGATCAACCACAGAAAAACGGAGATAGGTTGCAGGGGAGGCTACCTTTGCGCCAGCACCTGACCGATGCTCATGGGTCAATAGCCGATAACCATGCGAAAACCGAAGCGCAAATCGCCGCCAATCCACACGGGCGTCACCGTGAACAAACTCACCGCGAAATGAGAGAt                                                                                                                                                            >  2:988026/1‑239 (MQ=255)
                                                                                    tGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATTACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGGGATCa                                                                                                                                                          >  1:663978/1‑237 (MQ=255)
                                                                                       cgccCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATTAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCCCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACt                                                                                                                                                       >  1:949345/1‑237 (MQ=255)
                                                                                        ccccATTTTTTCCCCCGCAGACAAGAAAACACCGACCGGCAGGAAGAAGAAGATCGCTTCGCCGATCACCCACAGAAAATGGTAGATCTTTTGAGGGGAGGGGTACATTTGCGCAAGCTCCGGACCTTTACCCAGGGGAAAATCGCCGATCACATTGCAAAAACCGAGGACCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCt                                                                                                                                                    <  2:90643/238‑1 (MQ=255)
                                                                                        acctaTTTTTTCCCCCCCTCACAAGCAAACACCGCCGGGCAGGTAAAAGAAGATCGCTTCCCCGACCAACCACAGAAAAGCGTAGATCGTTTGCGGGGAAGGGTACATTGGCGCCAGCGTCTGCCGGTTGCTGAGGGGAAAACCGCCGACCACATTGAAAAAACCGAGGAACAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCt                                                                                                                                                    <  2:1422494/235‑1 (MQ=255)
                                                                                         cccATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGGAGAAGAAGATCGCTTCACCGCTCAACCACAGAAAATCGGAGATCGTTTGTAGGGACGGGTACATTTGGGCCAGCGCATGGCCGTTGCTCATAAGCAAATCGCCGATAACATTGCGCAAACCGAGGATCAAACAGCCGCTAATCAACTCAGGCAGCCACGTGAAGAAGCTCTCCGCGAAATGAGAGATCAAcgtct                                                                                                                                                    >  2:1441027/1‑234 (MQ=255)
                                                                                           cATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCAt                                                                                                                                                 >  1:1174820/1‑239 (MQ=255)
                                                                                            tttttttcCCCCGCGCACCACAAAATACCGCCCGGCAGGAAAAAGAAGATCGCTCCACCGACCAACCAAAGAAAATCGTAGATCTTTGGCGGGGAGGGGTACATTTGCCCCAGCGTCTGCCGGTTGCTCATGGGCAAACCGCCGACCACATGGCAAAACCCAAGGATAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATg                                                                                                                                                <  1:1386272/238‑1 (MQ=255)
                                                                                                 ttcccccgcgacccAGCAAACCCCGACCGGAAGTGAGAAAAAGATCGCTCCCCAGAAAACCCACAAAAAATCGTAGTTCGTTTGCAGGGAGGGTTACATTGCCGCCGGCTTCTGCCCGTTGCTCATGGGAAAATCCCCAACCACATTGCAAAACCGGAGGATAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCAtt                                                                                                                                           <  2:313458/227‑1 (MQ=255)
                                                                                                 gttCCCCGCTCACCAGAAAACACCGACCGGGTGGGAAAAGAAGGTCGATCCCCCGACCCCCAAAAGAAAATGGTAGTTTGTTGGAGGGGAATGCTACATTTCGTCCAGCGTCTGACGGTTCTTCAGGGGCAAAGCGCAGACCACATTGGAAAACCGAAGAACAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCAtt                                                                                                                                           <  2:1174820/238‑1 (MQ=255)
                                                                                                           gACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCAtattat                                                                                                                                  >  1:1205456/1‑238 (MQ=255)
                                                                                                                 cAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGggc                                                                                                                           >  1:1002737/1‑239 (MQ=255)
                                                                                                                      accgaccgGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCtt                                                                                                                      >  1:291159/1‑239 (MQ=255)
                                                                                                                      accgaccgGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCtt                                                                                                                      >  2:703736/1‑239 (MQ=255)
                                                                                                                          accgGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCcttttt                                                                                                                  >  1:1321087/1‑239 (MQ=255)
                                                                                                                              gagggaagaagaggaTCGCTTACCCGAAAAACAAAAAAAAGTCGTAGATGGTTGGAAGCGAAGGGTACATTGGGCCACGGGCGTGTCGTTGGCTTTGGGAAAACTCGGCGACCAGTTTGAAAACACCGAGGAAAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTAc                                                                                                               <  2:118831/232‑1 (MQ=255)
                                                                                                                                   tAGAAGAAAACCGCTACACCAATCAACCAAAGAAAGTCTGAGATCTTTTGAGGGGAGGGGTACTTTGGCGCCAGCGTCCGACGGTTCGTCAGGAAAAAAGCCACGAACACATGGAAAAAACAGAGAAACAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGct                                                                                                          <  2:321432/238‑1 (MQ=255)
                                                                                                                                        gaagaTCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTa                                                                                                     >  1:406975/1‑238 (MQ=255)
                                                                                                                                            aTCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGgcagca                                                                                                                                                                                           >  1:1243116/1‑148 (MQ=255)
                                                                                                                                            aTCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCaa                                                                                                 >  1:822142/1‑238 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATcac                                                                                                                                                                                                                                            >  1:743284/1‑97 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATcac                                                                                                                                                                                                                                            <  2:743284/97‑1 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  1:1029606/1‑239 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  2:649859/1‑239 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  1:344366/1‑239 (MQ=255)
                                                                                                                                                cTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTgc                                                                                            >  1:572626/1‑239 (MQ=255)
                                                                                                                                                        aTCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTcc                                                                                    >  1:759543/1‑239 (MQ=255)
                                                                                                                                                           aaCCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGt                                                                                 >  2:1096679/1‑239 (MQ=255)
                                                                                                                                                               aaaaaaatgcGTAGATGTTTTGGGGGAAAGGTTACATTGGGCCCGGCGTCTGCCGGTGGCTAAGGGGAAAGCCGCCGATCAAATTGCAAAACCCGAGAACCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGAc                                                                             <  1:243448/230‑1 (MQ=255)
                                                                                                                                                               aaaGAAAAGCGTAGATCGTTGGCGGGGAAGGGTACATTGGCGCCAGCTGCGGACCGTTGCTCAGGGGAAAACCGCGGACCACATTGCAAAACCGGGGAATAAACCCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGAc                                                                             <  1:571222/237‑1 (MQ=255)
                                                                                                                                                                caGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGAc                                                                             >  2:1204928/1‑238 (MQ=255)
                                                                                                                                                                caGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACATCCTCTTTATCAACCTGCGCCTTTTCGGTTGAc                                                                             >  2:1416298/1‑238 (MQ=255)
                                                                                                                                                                    aaaTCGTAGATGGTGTGCAGGGAAGGTTACATTGGCCCCAGCTCCCGCCCGTTGCCAATGGACAAACCGCCGACCAAATTGCAAAAACCGAGAATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAAt                                                                        <  1:1061426/239‑1 (MQ=255)
                                                                                                                                                                      attgTAGATCGTTTGGAGGGAGGGTTACATTTGCCCCAGCGTCTGACCTTTGCTCATGGGCAAACGCCCGATCAAATTGCAAAACCCGAGAAAAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCa                                                                      <  1:904355/236‑1 (MQ=255)
                                                                                                                                                                      aTCGTGTATCGTTTGCAGGGAAGGTTACATTTGCCCCAGCGTCTGACCGTTGCTAATGGACAAATCGCCGATCACATGGCAAAAACCGAGAAACAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCa                                                                      <  1:121761/239‑1 (MQ=255)
                                                                                                                                                                       tCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAg                                                                     >  2:987283/1‑239 (MQ=255)
                                                                                                                                                                          tAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAgc                                                                    >  1:1203153/1‑236 (MQ=255)
                                                                                                                                                                             aTCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGCCCGGTTGACGCAATCAGTGCTTg                                                               >  2:510348/1‑239 (MQ=255)
                                                                                                                                                                                 tttGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCaa                                                                                                                                                         >  1:314993/1‑145 (MQ=255)
                                                                                                                                                                                 tttGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAg                                                           >  2:679215/1‑239 (MQ=255)
                                                                                                                                                                                 tttGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAg                                                           >  2:480545/1‑239 (MQ=255)
                                                                                                                                                                                 cttGCAGGGAAGGGTACATTTGCGCCAGCGTCTTACCTTTGCTCATGGGCAAATCGCCGATCACAGAGCGAAAAACGAGGATCAAACCGCCGCTAATCAACGCGGACAGCAACGAGAACAATATCACCGCGAAATGAGGGATCAACTGCTCATCCCATTTCATATTATGCCGGGCAGCTTTTTTTAACTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGTTa                                                            >  2:1105502/2‑238 (MQ=255)
                                                                                                                                                                                   tGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGCTAGTa                                                         >  2:380754/1‑239 (MQ=255)
                                                                                                                                                                                            gggTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCAc                                                >  2:674859/1‑239 (MQ=255)
                                                                                                                                                                                               tACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAAc                                                                                                >  1:346439/1‑188 (MQ=255)
                                                                                                                                                                                               gaCATTTGCGCCATCGTCGCCCCGTTGCTCATGGGCAAATCGCCGACCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCGGCGCGGGCAGCAACGGGAAGAAGATCACCGCGCAATGAGTCATCAACTGCTCATGCGATTTCATATTATGCCGGGCGGCTTTGTTTAAATGCCCTTTATCCACCTGCGCCTGTCCTGTTGACGCACTCAGTGCTCGATCGTAATCACCCACGtt                                             >  2:1055824/2‑239 (MQ=255)
                                                                                                                                                                                                  aTTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGt                                          >  1:1349071/1‑239 (MQ=255)
                                                                                                                                                                                                       cgcCAGCGTCTGACCGTTGCTCATGGGCAAATGGCCGATCACATAGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGACGGCAGCAACGGAAAGAAGATCACCGCGAGATGAGAGATCAACTGCTCATGCCATTTCATATTATGCTGGGCGCGTTTTTTTACCTGCTCTTTAACACCCTGAGCCTGTCGGTTTGAAGCTATCAGTGCTTGATATTAGTCACCCAAGttagggacaa                                     >  2:753636/1‑231 (MQ=255)
                                                                                                                                                                                                         ccAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATc                                   >  1:1273771/1‑239 (MQ=255)
                                                                                                                                                                                                          cggCGTTTGACCGTTGCCCGAGGGCAAACCCCCGAACAAATGGAGAAACCCAGGGAACAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATca                                  <  1:1350907/237‑1 (MQ=255)
                                                                                                                                                                                                            gCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATcacc                                >  1:1157459/1‑239 (MQ=255)
                                                                                                                                                                                                               tCTGCCGGTTGCTAAGGGCCAAACCGCCAACCACATGGCAAAAACCGAGAAAAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCaccacc                             <  1:1041853/239‑1 (MQ=255)
                                                                                                                                                                                                                   ccggTTGCTCGTGGGCAAACCGCCGACAGCATTGCAAAAACCGAGAATAAACCCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGaa                         <  2:1362381/236‑1 (MQ=255)
                                                                                                                                                                                                                        tGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTg                     >  1:554441/1‑238 (MQ=255)
                                                                                                                                                                                                                        tGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTg                     >  1:1178923/1‑238 (MQ=255)
                                                                                                                                                                                                                        tGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTg                     >  1:444413/1‑238 (MQ=255)
                                                                                                                                                                                                                         gCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGc                    >  2:81751/1‑238 (MQ=255)
                                                                                                                                                                                                                          cTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGCTAGTAATCACCCACGTTGGTGCAAATCACCACCTGAAATTGccc                  >  2:1222484/1‑239 (MQ=255)
                                                                                                                                                                                                                           tCAGGGGCAAACCGCCGATCCCATTGCAAAACCCGAGGACAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGa                                                              <  2:1141074/194‑1 (MQ=255)
                                                                                                                                                                                                                                  cAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTgg          >  1:335340/1‑239 (MQ=255)
                                                                                                                                                                                                                                     aTCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCAc                                                    >  1:1141074/1‑194 (MQ=255)
                                                                                                                                                                                                                                         ccctaTCACATGGCAAAACCCGAGAATAAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAAc    <  1:595532/235‑1 (MQ=255)
                                                                                                                                                                                                                                             aTCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAg  >  1:1056941/1‑237 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
CCGGCAGCTGCTGCCCGAGCAGATAAGCGTTCATCAGCTGTGGAGAAACCAGTGTCACGCCAAGCACGATACCAAGGATCGGCGTGCCGCCCATTTTTTTCACCGCTGACCAGCAAATACCGACCGGCAGGTAGAAGAAGATCGCTTCACCGATCAACCACAGAAAATCGTAGATCGTTTGCAGGGAAGGGTACATTTGCGCCAGCGTCTGACCGTTGCTCATGGGCAAATCGCCGATCACATTGCGAAAACCGAGGATCAAACCGCCGCTAATCAACGCGGGCAGCAACGGGAAGAAGATCACCGCGAAATGAGAGATCAACTGCTCATGCCATTTCATATTATGCCGGGCGGCTTTTTTTACCTGCTCTTTATCAACCTGCGCCTGTCCGGTTGACGCAATCAGTGCTTGATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAG  >  NC_000913/4465542‑4466014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: