Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 373,637 T→G 19.9% G239G (GGT→GGG mhpF → acetaldehyde‑CoA dehydrogenase II, NAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913373,6370TG19.9% 150.4 / 5.2 76G239G (GGT→GGGmhpFacetaldehyde‑CoA dehydrogenase II, NAD‑binding
Reads supporting (aligned to +/- strand):  ref base T (18/39);  new base G (15/2);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 4.58e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAA  >  NC_000913/373401‑373872
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
aaaTCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTa                                                                                                                                                                                                                                               <  2:711222/239‑1 (MQ=255)
 aaacggCCGGACCTGGGACGAGTGACAATAGCGAGGAATTTCCGGAAACCGAATCCCGAGCCATTGAAGTCGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGCTTa                                                                                                                                                                                                                                               <  2:406926/233‑1 (MQ=255)
      gCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCCGGCTTACCTACCGGGtttaccc                                                                                                                                                                                                                                           >  2:472850/1‑232 (MQ=255)
      gCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTTCGGAACCGGGTATCg                                                                                                                                                                                                                                            >  2:394073/1‑236 (MQ=255)
         ggACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGaa                                                                                                                                                                                                                                      <  1:868132/239‑1 (MQ=255)
         ggACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGaa                                                                                                                                                                                                                                      <  2:1379936/239‑1 (MQ=255)
                  aCGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGt                                                                                                                                                                                                                             <  2:1053934/239‑1 (MQ=255)
                  aCGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGAACCGGGTTATCGCCTGAAACAGCGCGt                                                                                                                                                                                                                             <  2:1394854/239‑1 (MQ=255)
                   cgcgAGCCAAGATCGGTGAATGTAGTGAACCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGTTGATTGTGCTTAACACAGCAGGGCCACCGTTGATGATGCGTTACACGTTGTATGTATTGAGCGACGAAGCTTCTCACGATGATATCGAAGCCTCAATCAATGAAATTGCTGAGGCGGTGCAGGCTTAAGTACCGGGTTCTCGCCTGAAACAGCGCGTg                                                                                                                                                                                                                            <  2:761579/239‑1 (MQ=255)
                       tGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGAAGGCTTACGTACCGGGCTATCGCCTGAAAAAACGCGGGCGGt                                                                                                                                                                                                                        >  2:479516/1‑239 (MQ=255)
                              atCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTAAGTACCCGGTTATCGCCTGGAACCACGCGGGCCGTTTGGAGt                                                                                                                                                                                                                >  2:751051/1‑239 (MQ=255)
                                cGATGAATGTGCGAAAACCACTGCCCGAGCCATTGAAGTGGTGGGCGGGGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCGGCAGACCCACCGTTGATGATGCGTGACACGGTGTATGTAGTGAGCGACGAAGGTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTa                                                                                                                                                                                                              <  2:5900/239‑1 (MQ=255)
                                  aTGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTAt                                                                                                                                                                                                             >  1:1177629/1‑238 (MQ=255)
                                    gAATTTACGGAAGCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATccc                                                                                                                                                                                                          <  2:1354895/239‑1 (MQ=255)
                                     aagttACGGAAACCACCGCCCGAGCCATTGAAGTGGGGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATccc                                                                                                                                                                                                          <  2:457236/235‑1 (MQ=255)
                                      aTTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCg                                                                                                                                                                                                         <  1:386240/238‑1 (MQ=255)
                                       tttACGGAAACCACTGCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCa                                                                                                                                                                                                       <  1:296610/239‑1 (MQ=255)
                                         tACGGAACCCACTGCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCa                                                                                                                                                                                                       <  1:690376/237‑1 (MQ=255)
                                           cGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTg                                                                                                                                                                                                                            <  1:101004/215‑1 (MQ=255)
                                                accaccTCCCGAGCCATTGAAGTGGTGGGCGGAGCGGCAAAAGGGAAGGCGATTATTGTGCTTACCCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAATTTATCCCGCAGGATAAAcc                                                                                                                                                                                              <  1:1090166/239‑1 (MQ=255)
                                                aCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGTTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTAACGGGTTATCGCCTGAAACAGCGCGTGCAGGTTGAAGGTATCCCGCAGGATAAAcc                                                                                                                                                                                              >  1:1070978/1‑239 (MQ=255)
                                                aCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAAcc                                                                                                                                                                                              <  1:510695/239‑1 (MQ=255)
                                                aCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAAcc                                                                                                                                                                                              <  1:1113117/239‑1 (MQ=255)
                                                  cACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCgg                                                                                                                                                                                            <  2:422584/239‑1 (MQ=255)
                                                    ttacccGAGCCATTGAAGTGGTGGGCGGCGCGGGAAAAGGGAAGGCGATTAGTGTGCTTAACCCAGCAGAGCCGCCGTTGATGATGCGGGACACGGGGCATGTAGTGAGCGGCGAAGATTAAGAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGGGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTc                                                                                                                                                                                          <  2:225426/236‑1 (MQ=255)
                                                    ctgcccGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTc                                                                                                                                                                                          <  1:1378665/236‑1 (MQ=255)
                                                             ccATTGAAGGGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTAcc                                                                                                                                                                                  <  1:631746/238‑1 (MQ=255)
                                                                           ggcggcGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGGGCAGGTTGAAGGTATCCCGCAGGAGAAACCGGGCAATTTACCGGGCGTGGGGCAAtt                                                                                                                                                                   >  1:156299/1‑239 (MQ=255)
                                                                             cggcgcggcAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGGTTGAAGTTATCCCGGAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATtc                                                                                                                                                                  >  1:43722/1‑238 (MQ=255)
                                                                                   ggcAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGGGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGa                                                                                                                                                            <  1:437727/238‑1 (MQ=255)
                                                                                     cAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACt                                                                                                                                                          <  1:826448/238‑1 (MQ=255)
                                                                                             aaGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAg                                                                                                                                                  <  1:308868/238‑1 (MQ=255)
                                                                                               ggCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCCGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCgg                                                                                                                                              >  1:706816/1‑239 (MQ=255)
                                                                                                     tattGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAAAAGCGCGTGCAGGTTGAAGGTATCCCGCAGGGTAAACCGGTCCATTTACCGGGCGTGGGGCAATTTTCCGGACTGACAACAGCGGTctggc                                                                                                                                       >  2:463607/1‑239 (MQ=255)
                                                                                                          tgCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTGAACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGCGGCTGTGCAGGCCTACGTACCGGGTGATATGCTGATACATCACCTGTAGTTTGGAGGTATCTCGCCAGCTGAAACGGTCAATTTACCTGCTGTTGCGCACGTCTGCGGAGTTAAAACAACgacatagcaggga                                                                                                                                    >  2:594197/1‑227 (MQ=255)
                                                                                                                    agcagAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTTCGTACCGGGGTATTGCCTGAAACAGCGCGGGCAGGTTGGAGGTATCCCGCAGGATAAACCGGTCCATTTACCGGGCGTGGGGCCATTCTCCGGACTGAAAACAGCGGGCAGGCTGgaaggcgaaggc                                                                                                                         >  2:24058/1‑238 (MQ=255)
                                                                                                                       agagCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgc                                                                                                                       <  1:455634/237‑1 (MQ=255)
                                                                                                                       agagCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgc                                                                                                                       <  1:24058/237‑1 (MQ=255)
                                                                                                                       agagCCACCGTGGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgc                                                                                                                       <  2:1381793/237‑1 (MQ=255)
                                                                                                                           ccACCGTTGATGCTGCGTTACACCGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGgcgcagcgca                                                                                                                 <  2:411591/239‑1 (MQ=255)
                                                                                                                           ccACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCCGGCGTACCTACCGGGTTATCTCCTTAAACACCCGGGGCAGGTTGAAGGTATCCCGCCGGATAAACTGGTTAATTTTTCGGGCGTGGGGCAATTTTTCCGGCTGAAAAACCGCGTCTCGGCTGGAGTCGGAGgcgcagacaa                                                                                                                 >  2:684790/1‑235 (MQ=255)
                                                                                                                                   gatgatGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATctg                                                                                                          >  1:1394631/1‑238 (MQ=255)
                                                                                                                                                    tgtatgtaTTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCgg                                                                                            <  1:85206/234‑1 (MQ=255)
                                                                                                                                                       atgtatTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCt                                                                                     <  1:394073/238‑1 (MQ=255)
                                                                                                                                                       atgtagTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTc                                                                                    <  1:479253/239‑1 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGGCTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCCGCGCATTATCCGCCTGCCTATGCGGGCAACCTCGACATTa                                                                             >  1:455332/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTa                                                                             >  1:851611/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTa                                                                             >  1:607901/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGGGGGGCAATTCTACGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTa                                                                             >  1:1391144/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAACGCGTGCAGGTTGAAGTTATCCCGCAGGATAAACCGGGCCATTTTCCGGGCGTGGGGCAATTCTTCGGACTGAAAACAGAGGGCTGGCTGGGAGTCGAAGGGGCAGCGCATTATCTGCATGCCTCTGCGGGCAACCTCGACCTTa                                                                             >  2:387624/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTTTCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGGTAAACCGGTCAATTTTCCCGGGGGGGGGCAATTTTCCGGGCTGCAAACAGCGGGCTGGCTGGGAGGCGGAGGGGCCGCGCCTTATCTGCCCGCCTATGCGGGCAACCTCCAcccg                                                                              >  2:85206/1‑235 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGGAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACAtga                                                                             >  1:857534/1‑237 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGGCTGAAAACAGCGGGCTGGCTGGAAGACGAAGGCGCAGCGCATTATCTGCCTGCCCATGCGGGCAACCCCGCCATTa                                                                             >  2:505722/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGGTATCGCCCGAAACAGCGCGTGCCGTTTTAAGGTATCCCGCAGGATAAAACGGTCAATTTACCGGGCGTGGGGCCATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAAGCGAAGGCGCAGCGCATTATCTGCCTGCCCATGCGGGCAACCTCGCCATTa                                                                             >  2:826567/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTAACGGGGTATCGCCTGAAACAGCGCGTGCAGGTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTa                                                                             >  1:1285769/1‑239 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTAACGGGGTATCGCCTAAAAAAACGCAGGCAGGTTGAAGTTATCCCGCAGGATAAAACGGTCAATTTACCGGGCGTGGGGGAATTCTCCGGGCCGAAAAAAACGGTCTGGCTGGGAGACGAAGGCGCAAGGGATTATTCGCCGGCCATATGCGGGAACCCCGActata                                                                             >  2:1142147/1‑235 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAAGGTTACGTACCGGGTTATCGCCTGAAAAAAAGCGTGCAGTTTGTAGTTATCCCCGAGGATAAACCGGTCAATTTAACGGGGGTGGGGCAATTTCCCGGACCGAAAAAAGGGGACTGGCGGGAAAGCGAAAGGGCAGGGCACTATCTGCCCGCCCATGCGGTGAAcccgcaaaa                                                                               >  2:1070470/1‑230 (MQ=255)
                                                                                                                                                              gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAAGCTTACGTACCGGGTTATCGGCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTAACGGGAGTGGGGCAATTCTCCGGAATGAAAAAAGCGGTCTGGCTGGTAGTCGAAGGCGCCGCGCACTATctgcccgc                                                                                                     >  2:256034/1‑216 (MQ=255)
                                                                                                                                                                    cgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGGGCAGGTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTCCCGGGCGTGGGGGAATTTTCCGGACTGAAAACAGAGGTCTGGCTGGGAGTCGAAGGCGCAGCGCCTTTTCTTCCTGCCTTTGCGGGCAACCTCGACATTATGGCt                                                                        >  2:217871/1‑238 (MQ=255)
                                                                                                                                                                    cgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAAGCTTACGTAACGGGGTATCGCCTGAAACAGCACGTGGCGGTTGAAGGTATTCCGCAGGGTAAACCCGTCCATTTACCGGGGGTGGGGGAATTCTCCCGGCTGAAAACAGCGGGCTGGCGGGAAGTCGGAGGCGCGACGCATTTTCTTGCCGCCCAACCGCGCCACCCCGGCATTATgtcc                                                                        >  2:204456/1‑235 (MQ=255)
                                                                                                                                                                    cgaAGCTTCACAAGATGATATCGAAGCCTCAATAAATGAAATGGCTGAGGCGGTTGATGCTTACGCACCGGGCTCTACACCGTAAAAACGGCTGCAGGTTGAAGATATCCCGCAGGATAAACCGGTCAATTTACAGGGGGTGGGGCAATTCTCCGGCCTGCAAACAGCGACCCTGCTGGAGGGCGAAGGCGCAGCGCATTATCTGGCCGCCGCTGTGGGCAACCTCGGCCTTATTACtt                                                                       >  2:1059908/1‑239 (MQ=255)
                                                                                                                                                                          ttCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGt                                                                  <  2:1177629/238‑1 (MQ=255)
                                                                                                                                                                                 gatgatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGGACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGGAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGGGGGGCAATTCTCCGGGCTGAAAACAGCGGTCTGGCTGGAAGTCGGAGGCGCAGCGCATTATTCGGCTGCCTATGGGGGAAACCTCGACATTATGACTTCCAGTGCGCTGGc                                                          >  2:300775/1‑239 (MQ=255)
                                                                                                                                                                                    gatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGAc                                                       <  2:851611/239‑1 (MQ=255)
                                                                                                                                                                                     agatggatgacgcaatcaatTAAATTGCTGAGGCGGTGCAGGTTTACGTGCCGGTGTAGCGCCTGGAACTGCGCGTGCGGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGAGGGGCAAGTCTCCGGACTGAAAACAGCGGTCTGGCTCGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAATCTCGACATTATGACTTCCCGTGCGCTGGCgcc                                                       <  2:839775/226‑3 (MQ=255)
                                                                                                                                                                                                            aTGGCTGAGGCGGTGCAGGCTTTCGTACCGGGGTATCGCCCGAAACAGCGCGTGCAGTTTGGAGTTATCCCGCAGGGTAAACCGGGCAATTTACCGGGGGTGGGGCAATTCTCCGGACTGAAAACAGCGGGCTGGCTGGGAGTCGAAGGCGCAGCGCCTTATCTGGCTGCCGATGCGGGGAACCTCGACCATATGACTTCCAGGGCGCTGGCGACAGCGGGAAAAATGGCCCAgac                                  >  2:384895/1‑234 (MQ=255)
                                                                                                                                                                                                                  gAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcg                         <  1:1070470/239‑1 (MQ=255)
                                                                                                                                                                                                                    ggCGGTGTAGGCTTACGTACCGGGTTATCGCCTGACACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcgc                        <  1:1059908/238‑1 (MQ=255)
                                                                                                                                                                                                                    ggCGGTGGAGGGCTTAGGACCGGGTTTACGCCTGAAACAGGGCGTGCAGTTTGAAGGTTATCCGGAAGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcgc                        <  1:472850/238‑1 (MQ=255)
                                                                                                                                                                                                                         ggCACGCCTACCTACCGGGTTATCCCCCGAAACAACGGGGGCAGTTTTAAGTTCTTCCGCCAGATAAACAGGTCCATTTACCGGGCGTGGGGCAATTCTCCGGTCTGCAAACAGCGGTCTGGCTGGAAGGCGGAGGAGCACCGCAATATCTGCCTGCCTAACCGGGCAACCTCGACATTCAGGCGTCCAGTGCGCTGGCGACCGCGGAAAAAAGCACCCCGTCAcccggcccgcacgg                   >  2:161886/2‑225 (MQ=255)
                                                                                                                                                                                                                                 ttACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTGTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGaa            <  1:751051/238‑1 (MQ=255)
                                                                                                                                                                                                                                    cGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGGCACTGGCGCGCACGGCAGGAGAGGCg         >  2:1058668/1‑238 (MQ=255)
                                                                                                                                                                                                                                        ccGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATg    <  1:387624/239‑1 (MQ=255)
                                                                                                                                                                                                                                         cGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCTCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGCCAGAGGAAGCGGCATg    >  2:1272722/1‑238 (MQ=255)
                                                                                                                                                                                                                                         cGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATg    >  2:624503/1‑238 (MQ=255)
                                                                                                                                                                                                                                         cGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATg    <  1:624503/238‑1 (MQ=255)
                                                                                                                                                                                                                                          gggTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGaa  <  2:43722/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               
AAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCAGGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAAGTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCGGTCTGGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCCTATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAA  >  NC_000913/373401‑373872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: