Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 373,637 | T→G | 19.9% | G239G (GGT→GGG) | mhpF → | acetaldehyde‑CoA dehydrogenase II, NAD‑binding |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 373,637 | 0 | T | G | 19.9% | 150.4 / 5.2 | 76 | G239G (GGT→GGG) | mhpF | acetaldehyde‑CoA dehydrogenase II, NAD‑binding |
Reads supporting (aligned to +/- strand): ref base T (18/39); new base G (15/2); total (35/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.58e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAA > NC_000913/373401‑373872 | aaaTCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTa < 2:711222/239‑1 (MQ=255) aaacggCCGGACCTGGGACGAGTGACAATAGCGAGGAATTTCCGGAAACCGAATCCCGAGCCATTGAAGTCGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGCTTa < 2:406926/233‑1 (MQ=255) gCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCC‑GGCTTACCTACCGGGtttaccc > 2:472850/1‑232 (MQ=255) gCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTTCGGAACCGGGTATCg > 2:394073/1‑236 (MQ=255) ggACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGaa < 1:868132/239‑1 (MQ=255) ggACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGaa < 2:1379936/239‑1 (MQ=255) aCGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGt < 2:1053934/239‑1 (MQ=255) aCGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGAACCGGGTTATCGCCTGAAACAGCGCGt < 2:1394854/239‑1 (MQ=255) cgcgAGCCAAGATCGGTGAATGTAGTGAACCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGTTGATTGTGCTTAACACAGCAGGGCCACCGTTGATGATGCGTTACACGTTGTATGTATTGAGCGACGAAGCTTCTCACGATGATATCGAAGCCTCAATCAATGAAATTGCTGAGGCGGTGCA‑GGCTTAAGTACCGGGTTCTCGCCTGAAACAGCGCGTg < 2:761579/239‑1 (MQ=255) tGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGAA‑GGCTTACGTACCGGGCTATCGCCTGAAAAAACGCGGGCGGt > 2:479516/1‑239 (MQ=255) atCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTAAGTACCCGGTTATCGCCTGGAACCACGCGGGCCGTTTGGA‑Gt > 2:751051/1‑239 (MQ=255) cGATGAATGTGCGAAAACCACTGCCCGAGCCATTGAAGTGGTGGGCGGGGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCGGCAGACCCACCGTTGATGATGCGTGACACGGTGTATGTAGTGAGCGACGAAGGTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTa < 2:5900/239‑1 (MQ=255) aTGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTAt > 1:1177629/1‑238 (MQ=255) gAATTTACGGAAGCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATccc < 2:1354895/239‑1 (MQ=255) aagttACGGAAACCACCGCCCGAGCCATTGAAGTGGGGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATccc < 2:457236/235‑1 (MQ=255) aTTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCg < 1:386240/238‑1 (MQ=255) tttACGGAAACCACTGCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCa < 1:296610/239‑1 (MQ=255) tACGGAACCCACTGCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCa < 1:690376/237‑1 (MQ=255) cGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTg < 1:101004/215‑1 (MQ=255) accaccTCCCGAGCCATTGAAGTGGTGGGCGGAGCGGCAAAAGGGAAGGCGATTATTGTGCTTACCCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑TTTATCCCGCAGGATAAAcc < 1:1090166/239‑1 (MQ=255) aCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGTTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTAACGGGTTATCGCCTGAAACAGCGCGTGCAGGTTGAA‑GGTATCCCGCAGGATAAAcc > 1:1070978/1‑239 (MQ=255) aCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAAcc < 1:510695/239‑1 (MQ=255) aCCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAAcc < 1:1113117/239‑1 (MQ=255) cACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCgg < 2:422584/239‑1 (MQ=255) ttacccGAGCCATTGAAGTGGTGGGCGGCGCGGGAAAAGGGAAGGCGATTAGTGTGCTTAACCCAGCAGAGCCGCCGTTGATGATGCGGGACACGGGGCATGTAGTGAGCGGCGAAGATTAAGAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGGGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTc < 2:225426/236‑1 (MQ=255) ctgcccGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTc < 1:1378665/236‑1 (MQ=255) ccATTGAAGGGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTAcc < 1:631746/238‑1 (MQ=255) ggcggcGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGGGCAGGTTGAA‑GGTATCCCGCAGGAGAAACCGGGCAATTTACCGGGCGTGGGGCAAtt > 1:156299/1‑239 (MQ=255) cggcgcggcAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGGTTGAA‑GTTATCCCGGAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATtc > 1:43722/1‑238 (MQ=255) ggcAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGGGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGa < 1:437727/238‑1 (MQ=255) cAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACt < 1:826448/238‑1 (MQ=255) aaGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAg < 1:308868/238‑1 (MQ=255) ggCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCCGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCg‑g > 1:706816/1‑239 (MQ=255) tattGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCTGAAAAAGCGCGTGCAGGTTGAA‑GGTATCCCGCAGGGTAAACCGGTCCATTTACCGGGCGTGGGGCAATTTTCCGGACTGACAACAGCG‑GTct‑ggc > 2:463607/1‑239 (MQ=255) tgCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTGAACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGCGGCTGTGCA‑GGCCTACGTACCGGGTGATATGCTGATACATCACCTGTAGTTTGGA‑GGTATCTCGCCAGCTGAAACGGTCAATTTACCTGCTGTTGCGCACGTCTGCGGAGTTAAAACAACgacatagcaggga > 2:594197/1‑227 (MQ=255) agcagAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTTCGTACCGGGGTATTGCCTGAAACAGCGCGGGCAGGTTGGA‑GGTATCCCGCAGGATAAACCGGTCCATTTACCGGGCGTGGGGCCATTCTCCGGACTGAAAACAGCG‑GGCA‑GGCTGgaaggcgaaggc > 2:24058/1‑238 (MQ=255) agagCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGgcgc < 1:455634/237‑1 (MQ=255) agagCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGgcgc < 1:24058/237‑1 (MQ=255) agagCCACCGTGGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGgcgc < 2:1381793/237‑1 (MQ=255) ccACCGTTGATGCTGCGTTACACCGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGgcgcagcgca < 2:411591/239‑1 (MQ=255) ccACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCC‑GGCGTACCTACCGGGTTATCTCCTTAAACACCCGGGGCAGGTTGAA‑GGTATCCCGCCGGATAAACTGGTTAATTTTTCGGGCGTGGGGCAATTTTTCCGGCTGAAAA‑ACCGCGTCTCGGCTGG‑AGTCGGAGgcgcagacaa > 2:684790/1‑235 (MQ=255) gatgatGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATctg > 1:1394631/1‑238 (MQ=255) tgtatgtaTTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCgg < 1:85206/234‑1 (MQ=255) atgtatTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCt < 1:394073/238‑1 (MQ=255) atgtagTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTc < 1:479253/239‑1 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGGCTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCCGCGCATTATCCGCCTGCC‑TATGCGGGCAACCTCGACATTa > 1:455332/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTa > 1:851611/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTa > 1:607901/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGGGGGGCAATTCTACGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTa > 1:1391144/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAACGCGTGCAGGTTGAA‑GTTATCCCGCAGGATAAACCGGGCCATTTTCCGGGCGTGGGGCAATTCTTCGGACTGAAAACAGAG‑GGCT‑GGCTGGGAGTCGAAGGGGCAGCGCATTATCTGCATGCC‑TCTGCGGGCAACCTCGACCTTa > 2:387624/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTTTCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGGTAAACCGGTCAATTTTCCCGGGGGGGGGCAATTTTCCGGGCTGCAAACAGCG‑GGCT‑GGCTGGGAGGCGGAGGGGCCGCGCCTTATCTGCCCGCC‑TATGCGGGCAACCTCCAcccg > 2:85206/1‑235 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGGAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACAtga > 1:857534/1‑237 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGGCTGAAAACAGCG‑GGCT‑GGCTGGAAGACGAAGGCGCAGCGCATTATCTGCCTGCC‑CATGCGGGCAACCCCGCCATTa > 2:505722/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGGTATCGCCCGAAACAGCGCGTGCCGTTTTAA‑GGTATCCCGCAGGATAAAACGGTCAATTTACCGGGCGTGGGGCCATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAAGCGAAGGCGCAGCGCATTATCTGCCTGCC‑CATGCGGGCAACCTCGCCATTa > 2:826567/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTAACGGGGTATCGCCTGAAACAGCGCGTGCAGGTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTa > 1:1285769/1‑239 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTAACGGGGTATCGCCTAAAAAAACGCAGGCAGGTTGAA‑GTTATCCCGCAGGATAAAACGGTCAATTTACCGGGCGTGGGGGAATTCTCCGGGCCGAAAAAAACG‑GTCT‑GGCTGGGAGACGAAGGCGCAAGGGATTATTCGCCGGCCATATGCGGG‑AACCCCGActata > 2:1142147/1‑235 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑AGGTTACGTACCGGGTTATCGCCTGAAAAAAAGCGTGCAGTTTGTA‑GTTATCCCCGAGGATAAACCGGTCAATTTAACGGGGGTGGGGCAATTTCCCGGACCGAAAAAAGGG‑GACT‑GGCGGGAAAGCGAAAGGGCAGGGCACTATCTGCCCGCC‑CATGCGGTGAAcccgcaaaa > 2:1070470/1‑230 (MQ=255) gAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑AGCTTACGTACCGGGTTATCGGCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTAACGGGAGTGGGGCAATTCTCCGGAATGAAAAAAGCG‑GTCT‑GGCTGGTAGTCGAAGGCGCCGCGCACTATctgcccgc > 2:256034/1‑216 (MQ=255) cgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGGGCAGGTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTCCCGGGCGTGGGGGAATTTTCCGGACTGAAAACAGAG‑GTCT‑GGCTGGGAGTCGAAGGCGCAGCGCCTTTTCTTCCTGCC‑TTTGCGGGCAACCTCGACATTATGGCt > 2:217871/1‑238 (MQ=255) cgaAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑AGCTTACGTAACGGGGTATCGCCTGAAACAGCACGTGGCGGTTGAA‑GGTATTCCGCAGGGTAAACCCGTCCATTTACCGGGGGTGGGGGAATTCTCCCGGCTGAAAACAGCG‑GGCT‑GGCGGGAAGTCGGAGGCGCGACGCATTTTCTTGCCGCC‑CAACCGCGCCACCCCGGCATTATgtcc > 2:204456/1‑235 (MQ=255) cgaAGCTTCACAAGATGATATCGAAGCCTCAATAAATGAAATGGCTGAGGCGGTTGA‑TGCTTACGCACCGGGCTCTACACCGTAAAAACGGCTGCAGGTTGAA‑GATATCCCGCAGGATAAACCGGTCAATTTACAGGGGGTGGGGCAATTCTCCGGCCTGCAAACAGCG‑ACCC‑TGCTGGAGGGCGAAGGCGCAGCGCATTATCTGGCCGCC‑GCTGTGGGCAACCTCGGCCTTATTACtt > 2:1059908/1‑239 (MQ=255) ttCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGt < 2:1177629/238‑1 (MQ=255) gatgatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGGACCGGGGTATCGCCTGAAACAGCGCGTGCAGTTTGGA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGGGGGGCAATTCTCCGGGCTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGGAGGCGCAGCGCATTATTCGGCTGCC‑TATGGGGGAAACCTCGACATTATGACTTCCAGTGCGCTGGc > 2:300775/1‑239 (MQ=255) gatATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGAc < 2:851611/239‑1 (MQ=255) agatggatgacgcaatcaatTAAATTGCTGAGGCGGTGCA‑GGTTTACGTGCCGGTGTAGCGCCTGGAACTGCGCGTGCGGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGAGGGGCAAGTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTCGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAATCTCGACATTATGACTTCCCGTGCGCTGGCgcc < 2:839775/226‑3 (MQ=255) aTGGCTGAGGCGGTGCA‑GGCTTTCGTACCGGGGTATCGCCCGAAACAGCGCGTGCAGTTTGGA‑GTTATCCCGCAGGGTAAACCGGGCAATTTACCGGGGGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GGCT‑GGCTGGGAGTCGAAGGCGCAGCGCCTTATCTGGCTGCC‑GATGCGGGGAACCTCGACCATATGACTTCCAGGGCGCTGGCGACAGCGGGAAAAATGGCCCAgac > 2:384895/1‑234 (MQ=255) gAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcg < 1:1070470/239‑1 (MQ=255) ggCGGTGTA‑GGCTTACGTACCGGGTTATCGCCTGACACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcgc < 1:1059908/238‑1 (MQ=255) ggCGGTGGAGGGCTTA‑GGACCGGGTTTACGCCTGAAACAGGGCGTGCAGTTTGAAGGTTAT‑CCGGAAGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGgcgcgc < 1:472850/238‑1 (MQ=255) ggCA‑CGCCTACCTACCGGGTTATCCCCCGAAACAACGGGGGCAGTTTTAA‑GTTCTTCCGCCAGATAAACAGGTCCATTTACCGGGCGTGGGGCAATTCTCCGGTCTGCAAACAGCG‑GTCT‑GGCTGGAAGGCGGAGGAGCACCGCAATATCTGCCTGCC‑TAACCGGGCAACCTCGACATTCAGGCGTCCAGTGCGCTGGCGACCGCGGAAAAAAGCACCCCGTCAcccggcccgcacgg > 2:161886/2‑225 (MQ=255) ttACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTGTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGaa < 1:751051/238‑1 (MQ=255) cGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGGCACTGGCGCGCACGGCAGGAGAGGCg > 2:1058668/1‑238 (MQ=255) ccGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATg < 1:387624/239‑1 (MQ=255) cGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCTCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGCCAGAGGAAGCGGCATg > 2:1272722/1‑238 (MQ=255) cGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATg > 2:624503/1‑238 (MQ=255) cGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATg < 1:624503/238‑1 (MQ=255) gggTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGaa < 2:43722/239‑1 (MQ=255) | AAATCTGCCGGACCTGGCACGCGTGCCAATATCGATGAATTTACGGAAACCACTTCCCGAGCCATTGAAGTGGTGGGCGGCGCGGCAAAAGGGAAGGCGATTATTGTGCTTAACCCAGCAGAGCCACCGTTGATGATGCGTGACACGGTGTATGTATTGAGCGACGAAGCTTCACAAGATGATATCGAAGCCTCAATCAATGAAATGGCTGAGGCGGTGCA‑GGCTTACGTACCGGGTTATCGCCTGAAACAGCGCGTGCAGTTTGAA‑GTTATCCCGCAGGATAAACCGGTCAATTTACCGGGCGTGGGGCAATTCTCCGGACTGAAAACAGCG‑GTCT‑GGCTGGAAGTCGAAGGCGCAGCGCATTATCTGCCTGCC‑TATGCGGGCAACCTCGACATTATGACTTCCAGTGCGCTGGCGACAGCGGAAAAAATGGCCCAGTCACTGGCGCGCAAGGCAGGAGAAGCGGCATGAA > NC_000913/373401‑373872 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |