Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	921,243	G→T	6.3%	M299I (ATG→ATT)	macB →	macrolide ABC transporter peremase/ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	921,243	0	G	T	6.3%	270.9 / 2.8	78	M299I (ATG→ATT)	macB	macrolide ABC transporter peremase/ATPase
Reads supporting (aligned to +/- strand):  ref base G (32/41);  new base T (3/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

CGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTA  >  NC_000913/921013‑921478                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             cgcAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCCGGCCGCGGAAAAAAATCGGTCCTTT‑CTTACCATGCTGGGGGTTT‑TTTTCGGGTATTGGGT‑GGTGGTTTTCCTTTGTGGGGGTGGGTG‑CGGCGCCCCAAAAAAGGtggtggg                                                                                                                                                                                                                                      >  2:826545/1‑238 (MQ=255)  gcAATCCCGCCGCCATTGAAAAAGGGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCg                                                                                                                                                                                                                                     <  1:6911/239‑1 (MQ=255)        cccccGGCATTGAAAAACGGAATGCGACGTGCGGGATGGAACCTGTGGTCAAGACGGTGTCTGGGTGGCGGAAGGTTGTCAGCGGTTTTATCGAGGCGCTGACGATGGCATGGCAGGC‑GCTGGCAGC‑GAATAAAATGCGTTCTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGA‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATAtt                                                                                                                                                                                                                               <  2:882882/237‑1 (MQ=255)          tccGCCAGTGAAAAAGTGGATGTTACTGGCGGGACGGAACCTTTTGGCAACACGGTGTCTGGCTGGCGGGAGTTTGTCAGCGGTTTTAACGAGGCGCTGGCGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCg                                                                                                                                                                                                                             <  2:860385/238‑1 (MQ=255)          cccGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTTTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCg                                                                                                                                                                                                                             <  1:761740/239‑1 (MQ=255)                 ttGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTAtt                                                                                                                                                                                                                      <  1:947902/239‑1 (MQ=255)                     aaaaGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGt                                                                                                                                                                                                                   <  1:1034382/238‑1 (MQ=255)                     aaaaGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGt                                                                                                                                                                                                                   <  1:826545/238‑1 (MQ=255)                     aaaaGGGAATGTTACTGGCGGGACGGAACCTGTTGGCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGt                                                                                                                                                                                                                   <  2:1251383/238‑1 (MQ=255)                      aaaGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGAGGATATTCGTTCTATTGGTAc                                                                                                                                                                                                                 <  1:202186/239‑1 (MQ=255)                      aaaGTGAATGTGACCGGCGGGGCGGAGCCTGTGTGCAACTCGGTGTGTTGCTGGCGGCAGTTGGTCAGCGGTTTTAACGAGGCGCAGACGATGGCATGGCGGGA‑TCTGGCAGC‑GAATAAAATGCGTATTTTACTGACCATGGTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TGTCCATTGTCGTGGTGGGTGACGCCGCCAAGCAAATGGTGCTGGCGGATATACGTTCTATTGGTAc                                                                                                                                                                                                                 <  2:1011840/239‑1 (MQ=255)                            aaTGTTTCTGGGGGGACGGAAACTGTTTTTAACACGGTTGCTGGCTGGGGGCAATTTTGCAGCGGGTTTAACGAGGCGCTGACGATGGGATGGCGGGC‑GCTGGGAGC‑GAATAAAATGCGTTCTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTTG‑GTTCCATTTGCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATa                                                                                                                                                                                                            <  2:1325707/238‑1 (MQ=255)                            aaTGTTACTGGCGGGACGGACCCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATa                                                                                                                                                                                                            <  2:1220171/238‑1 (MQ=255)                              tGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGTGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGGATTGCGTCGGGGG‑TTTCCATTGTCGTGGTGGGGGACGCCGCCAAACAAATGGTGCTGGCGGAGATTCGTTCTATTGGGACGGATACTa                                                                                                                                                                                                         >  1:251777/1‑239 (MQ=255)                                      gcggggcgggACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGGCAGCGGGTTTTACGAGGCGCTGACGCTGGCATGGCGGGC‑GGTGTGCGG‑GAAAAAAAAGCGTACCTTTCTTGCCATTCTGGGGGTTATTTATC‑GTAATTG‑GTGCGTGGTTTTTCTTTCCGGTGGTGGGGGACGCCGCCGAACAAATGTTGCTGTTGGGTTTTGGTTTTATTGGTCCgcaacttatttgt                                                                                                                                                                                                    >  2:476724/1‑225 (MQ=255)                                                    gttgtCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGGGCTGGCGGGTATTCGTTCTATTGGTACGGATAATATTTATGTCTTTCCCGGGAAAGa                                                                                                                                                                                   >  1:897989/1‑239 (MQ=255)                                                         cAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGAATTGAGTCAGTGG‑TTTCCCTTGTCGTGGTGGGGGACGCCGCCAAACACATGGGGCGGGCGGATATTCGTTTTATTGGTAAGAATACTATTGATGGCGATCCCGGGAAAGATTTTg                                                                                                                                                                              >  1:425554/1‑239 (MQ=255)                                                               ggTGTCTGGCTGGCGGCAGATTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGCGGCGGGCA‑C‑GCATAAAATAAGTTCTTTTCTGACCGTGGTGGGGGTTA‑TTATC‑GGTCATGAGTAGGGGG‑TTTTCATTGACGCGGTGGGTGATGCAGCCAAACAAATGGCAGTTGTGGATATTAGCTCTTTTTGTTCGGATACTATAGATTCGCATCCCGGGAAAGATTTTTGctgcg                                                                                                                                                                        >  2:1134300/1‑235 (MQ=255)                                                                 ggtctgtggggcggcAGTTTGTCAGCGGTTTTAACTAGGCGCTGACGATGGCTTGACGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTGTCTGACCATTCTGGGGATTA‑TTAGC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTAGCCCGGGAAAGATTTTTGCGATGa                                                                                                                                                                       <  2:919863/229‑1 (MQ=255)                                                                  gtCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGGACTTTACTAACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCCAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATTCCGGGAAAGATTTTGGCgcgcacg                                                                                                                                                                     >  1:1039623/1‑233 (MQ=255)                                                                   tCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACg                                                                                                                                                                     <  1:466658/238‑1 (MQ=255)                                                                    ctggctggCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGGACTTTACTGACCATGCCGGGGATTA‑TTATC‑GGTACTGCGCTGGTGG‑GTTCCATTGTCGTGGTGGGGGAGCGCGCCAAACATATTGTGCCTGCGGATTTTTCGTCTATTGGCACGGATACCATTTTTGTTTTTTCCGGGATAGAtttttgggactgggtt                                                                                                                                                                   >  2:549433/1‑227 (MQ=255)                                                                     tggctggcGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATc                                                                                                                                                                  <  1:1111047/239‑1 (MQ=255)                                                                     tggctggcGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATc                                                                                                                                                                  <  1:645935/239‑1 (MQ=255)                                                                           gcggcAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GCATAAAATGCGTACTTTACTGACCATGCTGGGGGTTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGGGGGTGACGCCGCCAAACAAAAGGGGGTGGCGGAGTTTTGTTCTGTTGGTACGAACACTTTTTAATTCTATCCCGGGGAAGATTTTTGCGCTGTAGATCCggcat                                                                                                                                                            >  2:127420/1‑235 (MQ=255)                                                                                       cAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGgcg                                                                                                                                                 <  1:425903/238‑1 (MQ=255)                                                                                                                atggcatggcGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCa                                                                                                                       >  1:541320/1‑238 (MQ=255)                                                                                                                 tggcatggcGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCAt                                                                                                                      <  1:1383240/238‑1 (MQ=255)                                                                                                                       ggcgggc‑gCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATTGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCaaaaa                                                                                                               >  1:585544/1‑239 (MQ=255)                                                                                                                       ggcgggc‑gCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTTGCGATGACGATCCGCAATATCAACCGGGGCTGAAGTACGACGA‑CTTAATCGCCATCCaaaa                                                                                                                >  1:504293/1‑238 (MQ=255)                                                                                                                       ggcgggc‑gCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCaaaaa                                                                                                               <  2:1202492/239‑1 (MQ=255)                                                                                                                       ggcgggc‑gCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGGGGGTGACGCCGCCCAACAAATGGTGCTGGCGGATATTCGTTCTATTTGTACGAAAACTATTTATGTCTATACCGGGAAAAATTTTTGGGGTGACAGTCCCCCATTACACCAGGCGCCGAAAGACGACCACCTTAATCGcctcacaaaa                                                                                                                >  2:1130185/1‑231 (MQ=255)                                                                                                                                  ggCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTggg                                                                                                     <  1:225338/239‑1 (MQ=255)                                                                                                                                         gAATAGAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGc                                                                                                 <  2:1040598/237‑1 (MQ=255)                                                                                                                                         gAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGc                                                                                                 <  1:1130185/237‑1 (MQ=255)                                                                                                                                             aaaaGGCGTTCTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCTATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATAGATGTCTAACCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCAAAAAACAACCGTGGGTCGCCTCAGc                                                                                           <  1:1419131/239‑1 (MQ=255)                                                                                                                                                     tACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCt                                                                                    >  1:107254/1‑238 (MQ=255)                                                                                                                                                        tttaaggACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTGTCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATGCTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCg                                                                                <  2:107254/233‑1 (MQ=255)                                                                                                                                                               aCCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGGTGTTTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATAGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCggtgcgca                                                                         >  2:1352166/1‑232 (MQ=255)                                                                                                                                                               aCCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCa                                                                         >  1:151485/1‑239 (MQ=255)                                                                                                                                                                  aTGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCaaa                                                                       >  1:623678/1‑238 (MQ=255)                                                                                                                                                                  aTGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCaaa                                                                       >  1:439184/1‑238 (MQ=255)                                                                                                                                                                  aTGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCaaa                                                                       >  1:777322/1‑238 (MQ=255)                                                                                                                                                                    gCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCaaaa                                                                      >  2:1056722/1‑237 (MQ=255)                                                                                                                                                                    gCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAc                                                                     >  2:385740/1‑238 (MQ=255)                                                                                                                                                                         ggATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTAtt                                                                                                                                                                                                       <  1:233284/104‑1 (MQ=255)                                                                                                                                                                         ggATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTAtt                                                                                                                                                                                                       >  2:233284/1‑104 (MQ=255)                                                                                                                                                                           atta‑ttATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGGCGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcc                                                              <  2:581422/238‑1 (MQ=255)                                                                                                                                                                           atta‑ttATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcc                                                              <  1:686530/238‑1 (MQ=255)                                                                                                                                                                           atta‑ttATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcc                                                              <  1:476724/238‑1 (MQ=255)                                                                                                                                                                           atta‑ttATC‑GGTATTGCGTCGGTGA‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCAGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcc                                                              <  1:549433/238‑1 (MQ=255)                                                                                                                                                                           atta‑ttATC‑GGTATTGAGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcc                                                              <  1:1126982/238‑1 (MQ=255)                                                                                                                                                                           atta‑ttATC‑GGGATTGGGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcc                                                              <  2:1389957/238‑1 (MQ=255)                                                                                                                                                                                      ggTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGGTATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTTGAGGACGACGA‑CCTAATCGCCCTCCCAAAACAACCGTGGGTCGCCCCCCCCACCCCTGCCGTCCCGCAAAACCTGCCACTGCtttttt                                                     >  2:1059329/1‑235 (MQ=255)                                                                                                                                                                                      ggTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTtata                                                     >  1:242711/1‑238 (MQ=255)                                                                                                                                                                                      ggTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATaa                                                    <  1:1277805/239‑1 (MQ=255)                                                                                                                                                                                           tGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAatg                                               >  2:469474/1‑239 (MQ=255)                                                                                                                                                                                           tGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTAGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAatg                                               >  1:1020982/1‑239 (MQ=255)                                                                                                                                                                                           tGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCCATTGGTACGAATCCTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGGACGACGA‑CTTAATTGCCATCCAAAAACAACCGTGGGTCGCCTCATCCCCCCCTGCCGTCTCGCAAACACTGCGCCCGCGTTATCACCatg                                               >  2:849635/1‑239 (MQ=255)                                                                                                                                                                                               tCGGTGG‑TTTCCATTGTCGTGGCGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAACAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCACAACCTGCGCCTGCGTTATAACAatgttga                                           >  1:1135161/1‑239 (MQ=255)                                                                                                                                                                                                  gtgg‑tTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATTGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGAGGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATcagc                                                                                                                                                      <  2:104571/130‑1 (MQ=255)                                                                                                                                                                                                  gtgg‑tTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATTGTGCTGGCGGATATTCGTTCTATTGGTACGAATAATATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATTCGCAATATcagc                                                                                                                                                      >  1:104571/1‑130 (MQ=255)                                                                                                                                                                                                  gtgg‑tTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcctg                                                            >  1:362083/1‑219 (MQ=255)                                                                                                                                                                                                  gtgg‑tTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAAcctgcgcctg                                                            <  2:362083/219‑1 (MQ=255)                                                                                                                                                                                                  gtgg‑tTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAatgttgatgt                                        >  2:1105840/1‑239 (MQ=255)                                                                                                                                                                                                            aTTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATTTTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAg                               <  1:1084174/239‑1 (MQ=255)                                                                                                                                                                                                            aTTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAg                               <  1:780642/239‑1 (MQ=255)                                                                                                                                                                                                                   tggtggGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAAt                        >  2:1299777/1‑239 (MQ=255)                                                                                                                                                                                                                     gtgggtgACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAAc                                                   <  1:1161289/210‑1 (MQ=255)                                                                                                                                                                                                                     gtgggtgACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAAc                                                   >  2:1161289/1‑210 (MQ=255)                                                                                                                                                                                                                     gtgggtgACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATg                       >  2:1071231/1‑238 (MQ=255)                                                                                                                                                                                                                          tgACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGa                 >  1:1095710/1‑239 (MQ=255)                                                                                                                                                                                                                                 gccAAACAAATTGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGa          <  2:585544/239‑1 (MQ=255)                                                                                                                                                                                                                                      acaaaTGGTGCTGGCGGATATTCGTTCTATTGGTACGaa                                                                                                                                                                                                              <  1:652334/39‑1 (MQ=255)                                                                                                                                                                                                                                        aaaTGGTGCTGGCGGATATTCGTTCTATTAGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTAtttt   <  1:1134300/239‑1 (MQ=255)                                                                                                                                                                                                                                         aaTGGTGCTGGCGGATCTTCGTTCTATTGGCACGAATAATATTGATGCCTATCCCGGGATAGATCTTGGCGATGAAGATCCGCAATAGCAGCAGGCGCTTGAGTACGACGA‑CTTAAACGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGATGTCAGTGCCAATGGCGTGAGCGGCGATTATTTTa  <  2:657803/239‑1 (MQ=255)                                                                                                                                                                                                                                         aaTGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTa  <  2:439184/239‑1 (MQ=255)                                                                                                                                                                                                                                         aaTGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTa  <  1:127420/239‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             CGCAATCCTCCCGCCATTGAAAAAGTGAATGTTACTGGCGGGACGGAACCTGTTGTCAACACGGTGTCTGGCTGGCGGCAGTTTGTCAGCGGTTTTAACGAGGCGCTGACGATGGCATGGCGGGC‑GCTGGCAGC‑GAATAAAATGCGTACTTTACTGACCATGCTGGGGATTA‑TTATC‑GGTATTGCGTCGGTGG‑TTTCCATTGTCGTGGTGGGTGACGCCGCCAAACAAATGGTGCTGGCGGATATTCGTTCTATTGGTACGAATACTATTGATGTCTATCCCGGGAAAGATTTTGGCGATGACGATCCGCAATATCAGCAGGCGCTGAAGTACGACGA‑CTTAATCGCCATCCAAAAACAACCGTGGGTCGCCTCAGCCACACCTGCCGTCTCGCAAAACCTGCGCCTGCGTTATAACAATGTTGATGTTGCTGCCAGTGCCAATGGCGTGAGCGGCGATTATTTTA  >  NC_000913/921013‑921478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑