| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 989,945 | A→G | 17.2% | S138P (TCA→CCA) | pncB ← | nicotinate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 989,945 | 0 | A | G | 17.2% | 123.5 / 2.5 | 71 | S138P (TCA→CCA) | pncB | nicotinate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (24/32); new base G (7/6); total (32/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTG > NC_000913/989709‑990182 | tCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagc < 2:66118/239‑1 (MQ=255) ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagcga < 1:956577/239‑1 (MQ=255) ccATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagcga < 1:1240384/239‑1 (MQ=255) cATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagcgat < 2:4742/239‑1 (MQ=255) tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagcgatag < 1:532502/239‑1 (MQ=255) tCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagcgatag < 1:675118/239‑1 (MQ=255) ggCGTGAGGGAAAGCCGACGCGGCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagcgatagcg < 1:800664/239‑1 (MQ=255) cGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGagcgatggcga < 2:1266383/238‑1 (MQ=255) cGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCAc < 1:333845/238‑1 (MQ=255) gATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCAACAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCTATAGCGATGTACCATTTCAc < 1:190770/228‑1 (MQ=255) gCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGGGGGCGGTAGCGATGTACCATTTCACTGATAACC‑GCCAGCaaaagga > 1:1288675/1‑235 (MQ=255) gCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAACGGCGAGAAGTGGACTAATTTGCTTTCCTGCGGGGCGGGGGGGTGGGCC‑ACC‑TCGGCCAGCGGAGGGCGATAGGCATGGAACATTTCTCTGATACAC‑Gccccggcactta > 2:190770/1‑229 (MQ=255) gTGCCCACAAACAAGGATTCGTGTGGAAGACGCTTAACGAGGGTTTTTTGTACTTCGCGAAAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATACCAAGACCGGCGGTTACCCCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCGGCGGTGGGCGATAGCGATGTACCATTCCACGGATAACC‑GCCAGCAAAGGAACtt < 2:194986/238‑1 (MQ=255) cccACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGACGACTAATTTTCTTTCCCGCGTGTCGGGGGGTTGCGCA‑ACC‑TCGGCCTGCGGGGAGCGGTAGCGATGTACCCTTTCACTGATAACC‑GCCCGCAAAGGAACTTcccc > 1:964644/1‑238 (MQ=255) cacaAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCCGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCaga > 1:213677/1‑239 (MQ=255) aaaccgggtctctggTTGCAGATGCTTACGTGTGGTTTGTGGTACACCGCGAGAAAACCGGCGCCGGGTGCCAAAACCCTCCAGATGGAACCGCGACATACCAAGCCCGGCGTTTCGCGCCGGGAGGGCGACTATTTTGCTTTCCCGCGTGTGGAGGGCTTGCAAA‑ACG‑TCGGCCTGCGGTGGGCGACGGAGATGTTCCATTGCCCGGATAGCC‑GCCAGCAAAGGAACTTCCCAGAGGa < 2:708095/225‑1 (MQ=255) gctgccggttgagggcgtttacagATGGTTTCTTGTCCTTCTGAAGAAAAACGGGGACGGGTGCCAAAACACATAAGAGGGAGGCGTGACATATCAAGCCGGGGGGTTAACGCGGAGAAGGCAACAAATTGGCTTTCCGGCGTGTAGAGGGCTTGCGCA‑ACG‑TCGGCCTGGGGTGAGCAATAGCGATGTCCCATTTCACTGAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACtt < 2:689661/215‑1 (MQ=255) ccTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAACCGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGCCCGGCGGTTACCGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGaa < 1:583861/216‑1 (MQ=255) cTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGACGACTAATTTGCTTTCCCACGTGGCGAGGGGGTTCGCA‑ACG‑TCGGCCCGCGGGGAGCGATAGGGATGTACCATTTCCCTGATAACCACCCCGCAAGGAAATTCCCCAAAGGATGA‑TTCAcac > 1:1358054/1‑236 (MQ=255) gTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGCGAAGTCGACTAATTTGCTTTTCAGCGGGGCGGGGGGTTGCGCC‑ACG‑TCGGGCTGCGGTGAGCGGTAGCGATGTACCATTTTACTGATAACC‑GCCCGCAAAGGAACTTTCCAGAGGATGACTTCACGcccc > 1:501511/1‑237 (MQ=255) aCGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGGGGGCTTGCGCA‑ACG‑TCGGCCCGCGGGGAGCGATAGGGGTGTAACCTTTCACTGATAACC‑CCCAGCAAAAGAACTTTCCCGAgggcggctcttatacacatctgt > 2:583861/1‑217 (MQ=255) aCGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAACGCCGAGAAGACGACTAATTTTGTTTCAAGCGTCTCGGGGGGTTGCGCC‑ACG‑TCGGCCTGCGGCGGGCGATAGCGCTGGAACATTTCCCAGGAAACA‑GCCAGCAAAAGGAAGTCCCAGGGGATGACTTTACCCCTCCGGccc > 2:1241610/1‑237 (MQ=255) aCGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGGTAAAGCCGAGAAGTCGACTAATTTTCTTTTCAGCGTGTCGAGGGATTGCGCA‑ACG‑TACGCCTGCAGTGAGTGGTAGCGGTGGACCATTTTCCTGAGAACA‑CCCAGCCAAAGAAGTTTCCAGGGGAGAGCTTCACAcccacggccg > 2:605814/1‑230 (MQ=255) tAACGATGGTTTCTTGTCCTCCGCGAGAAAACCGGGGACGGGTGCAAAAATCCATCAGGGGAAACCGCGACATATCAGGACCGGGGGTTACCGCCGAGAGGTCGACTAATTGGCTTTCCAGCGTGTGGAGGGCTTGCGCA‑ACG‑TCGGCCGGCGGTGAGCGATAGCGATGTACCATTTCACTGAAAGCC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTaaa < 2:1165655/239‑1 (MQ=255) acggtggtttcgtagcgtcaccgAAAAAAACGTGGACGGGTCCCAAAACCCAGCAGTGGGAACCGCGACTAATCAGCGCCGGCGTTTACCCCCGAGATGTCGACTAATTTGCTTCCCGGGTGGTCGAGGGCTTGCCCA‑GCG‑TCGCCCGGGGGGGAGCGATAGGGATGTACCATTTCACTGAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAAc < 2:501511/217‑1 (MQ=255) ggTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAATCGACTAAATTGCTTTCCAGCGTGTCGGGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGCTGGACCATTTCACTGATAAAC‑GCCAGCAAAGGAAATTCCCAGAGGATGACTTCACGCCACGGGCCGCCTAAACGaaaa > 1:1206731/1‑236 (MQ=255) tttCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGAATAATTTTCTTTCCAGCGGGTCGGGGGCTTGCGCA‑ACG‑TCGGCCCGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAAGAACATCCCCGAGGATGACTTCACGCCCACGGCCGCTTaaaagcaaaaa > 2:1274740/1‑230 (MQ=255) tCTTGTACTTCGCGAGAAAACCGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCACGACCGGCGGTTAAAGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGCGGGGTTGCCCA‑AAGTTCGGCC‑ACGGGTAGCGATACGGGTGTACCATTTCTATGACAAAC‑GCCCCCAAAAGAACTTCCCCGACGAGAGCCTCCCGCCCCGGGCCggctacactaaactacc > 2:1326521/1‑222 (MQ=255) cTTGTACTTCGCGAAAAAAACGGCGACGGGTGCAAAAACCCATCAGATGAAAGCGCGACATATCAAGACCGGCGTTTACCGCCGAGAATTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGGAATAGCGATGTCCCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGc < 1:71086/239‑1 (MQ=255) ttGTACTTCGCGAGAAAAAGGGCGACGGGGGCCAAAATCCATCAGAGGGAAGCGCGACATATCAAGCCCGGGGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑t < 1:38408/239‑1 (MQ=255) gggtctgggggagaAAAACGGGGGCGGGTGCAAAAACCCATAAAGGGGAAGCGTGACATAACAAGCCCGGCGGTTACCGCCGAGAAGTCGCCTTATTTGCTTCCCAGGGTGTGGGGGGTTTGCGCA‑CCG‑CCGGCCGGCGGGGGGCGATAGCGATGTCCCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑tt < 2:1162615/229‑1 (MQ=255) ttCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAAAGCCCAGAAATCGACTAATTTGCTTTCCACCGTGTCGGGGGCTTGCGCA‑ACG‑CCGGCCCGCGGTGGGCGGTAGCGATGTTCCATTTCACCGATAAAC‑CCCCGCAAAGGAACTTACCACAGGAATACTTCACGCCACGGGGCCCTTAAACGAATATCCACC‑CTGccct > 2:719420/1‑237 (MQ=255) tccgcgAAAAAAACGGGGGGGGGTCCAAAAATCCATAAGAGGAAAGCGCGACATAACAAGCCGGGCGGTTACCGCGGAGAATCCGACTAATTTGCTTTCCAGCGTGTAGAGGGTTGGCGAA‑CCG‑TCGCCCTGGGGGGAGAGATAGGGATGTCCCATTTCCCTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCAt < 2:1318142/237‑1 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGTTGGAAGCGCGACATATCAAGACCGGCGGCTAAAGCCGAGAAATCTGCTAATTTGCTTTCCAGCGTGTAGAGGGCCTGCGGA‑ACC‑GCGGGCCGCGGTGTGCGAAAGCGATGTGCCCTTTTTCTCACAACA‑GCCCGCAAAGGGACTTGCCCGAGGATGTCATTACGCCACCCGCCCCCTTAAAGAATAACACAC‑TTGCCCTTa > 2:160932/1‑239 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTTAAGCCGAGAAGTCGGCAAATTTGCTTTTCAAAGGGTCGCGGGATTGCGCC‑ACG‑TGGGCGTGCGGGGGGCGAAAGGGAGGTACCATTTCAATGATAACC‑GCCAGCAAAGGAACTTCCCCGAGGATGAGTTCACGCCACGGGCGCCGTAAACGaaactcccacatgcgatta > 2:588592/1‑222 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGGGGCGAGGGCTTGCGCA‑ACG‑GCGGCCTGCGGTGAGCGGTAGCGAGGTACCATTTCACTGATAACC‑GCCCGCCAAAGAAATTCCCAGAGGATGACTTCACGCCCCGGGCCGCTTAAACGAATATACAGC‑TTTCCCTTa > 2:761944/1‑239 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGGCGACTAATTTGCTTTCCAGCGTGTCGCGGGCTTTCGCC‑ACG‑GCGGCCTGCGGTGAGCGGTAGCGGTGTACCCTTTTACTGGTAACC‑GCCAGCAAAGGAACTTCCCCGAGGATGACTTTCCCCCACGGGCCGCCTAAAAGAATATCCCGC‑CTgtccttt > 2:1374695/1‑233 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACCCCCAGAAGTCGACAAATTTGCTTTCAAACGTGCCGTAGGCTCGCGCA‑ACG‑TCCGCCTGCGCGCAGGCATAGCGGAGTTCCATTTCACCGATAACA‑CCCCGCCAAAGAACTTCCCCAAGGAGAACTTCCCCCCCCCGGCCGCGTAAAACAACATCCACCATTGCCAtt > 2:738154/1‑239 (MQ=255) cgcgAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGCTAACGCCGGGCAGACGACTAATTTTATTTTCCGCGTGTCGGGGGCGTGCGCACACG‑‑CCGCCTGCGGTGGGCGCTAGCGATGTAGCATTTTTCTGATAAGC‑GCCCGCAAAGGAACTTCCCTGAGGCTGACTTCACGCCCCGAGCCGCTGTAAAGAAAATCCAAC‑CTGGCATTa > 2:1051256/1‑239 (MQ=255) ggTGCCAAACACAAACAGAAGGAAGCGCGACATAGAAAGCCCGGCGGTTAACGCTGAGAATCCGACTAATTGCCTTTCCAGCGTGGGGAGGGCTGGGGAA‑ACG‑TGGGCCTGGGGAAAGGGATAGCAATGTCCCATTTCGCTAAAACCC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGAc < 2:1218247/237‑1 (MQ=255) cgcgACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGGGTCGAGGGGTTGCGCA‑ACG‑GCGGCCTGCGGTGAGGGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGGACTTCCCAGAGGATGACTTCACGCCACGGGGCGCTTAAACCAATATc > 1:141332/1‑182 (MQ=255) gACATATCAAGACCGGCGGTTTACGCCGGGAAGTCGACTAAATTGCTTTCCAGCGTGTCGAGGGCCTTCGCC‑ACG‑CCGGCCCGCGGTGAGCGAAAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGGACTTCCCCGAGGATGACTTCACCCCACGGGCCGCCTCAACGAATATCCCAC‑TTTCCATTATCGTGTTGCACGGGGGCTTTTTCCGGGGTAAAACGGGACTcgcgga > 2:1049122/1‑236 (MQ=255) aaagtaccAAGACCGGGGGTTAACGCCGAGACGCCCACTATTTTGCTTTCGAGCGTGCCGAGGTCTTGCGAA‑ACG‑GCGCCCTGCGGTGTGGGATAGGGATGTCCCATTTCACTAATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 1:801429/232‑1 (MQ=255) atCAAGACCGGCGGTTAACGCCGAGAAGTCGACTATTTTGCTTCCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTCCCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGAt < 1:1260426/141‑1 (MQ=255) ggggTTAGCGCGGAGAGGGCGACTAATTTGCTTTCCAGCGTGGGGGGGGCTTGCGCA‑CCG‑TCGGCGTGCGGGGGGGAATAGCGATGTACCATTTCACTGAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 2:979529/236‑1 (MQ=255) ggggTTAACGCCAAGAGGCGAACTAATTTTCTTTCATGTTTTTCGGGGTCTTGCTAA‑ACG‑TCGGCCGGCGGTGAGGGATAGCGATGTACCATTCCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 2:1206731/236‑1 (MQ=255) gcgttaaaCGCCGGAAATTGGACTATTTTGCTTCCAGGGGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGGGGTGAGCGATGGCGATGTACCATTTCACTAATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 1:647794/232‑1 (MQ=255) gcgtTTAACGCCGGTAAGTCGACTAATTTGCTTTCCGGCGTGTCGGGGCCTTGCGCA‑ACG‑TCGGCGTGCGTTGAGCGATAGCGATGTACCATTTCCCGGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 1:767947/238‑1 (MQ=255) gcggTTAACGCCGCGAAGTCGACTAATTTGCTTTCCAGCGGGTCGAGGGCTTGCGCC‑ACG‑TCGGCCTGCGGTGGGCGATAGCGATGTACCATTTCACTGATAAAC‑GCCAGCAAAGGAACTTCCCAGAGGGTGACTTCACGCCACCGGCCGCCTAAACGAAAATCCCGC‑TTGCCATTATCGTTGGACACGGGGACTTGTTTCGGGTTAAAAAGGAACTCTCGCAACAAGTGAAGATaatg > 2:530600/1‑236 (MQ=255) cggttaaaggcggaaaggTCGACTAATTTGCTTTCCAGCGGGCGGGGGGCTGGCGAA‑AGG‑TGGGCCTGCGGGGAGCGATAGCGATGTCCCTTTCCACTGAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGt < 2:579767/221‑1 (MQ=255) gggTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTc < 1:1374695/237‑1 (MQ=255) cggTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGGGTCGAGGGCTTTCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTTACTTATAACC‑GCCAGCAAAAGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCCTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGGGACTTGTTTCCGGTTTAAGCGGGACTCCCCTAACCAGCTAATATTGTCg > 2:80632/1‑239 (MQ=255) ggaaaagcggattagtttGCTTTCCGGGGTGTGGGGGCCTGGCGCA‑ACG‑GCGGCGTGCGGGGAGCGATAGCGATGTACCATTTCACTAATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaag < 2:1288675/223‑1 (MQ=255) gaAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTaaagaaag > 2:362211/1‑239 (MQ=255) aaGTCGACTATTTGCCTTTCCGGCGTTTCGAGGGGTTTGGCA‑ACG‑TGGGTCGGTGGTGAGCGATAGCGATGTCCCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg < 1:400397/239‑1 (MQ=255) aaGTCGAATAATTGTGTTTCCGGCGTTTCGGGGGCTTGCGAA‑CCC‑CCGGTCTGCGGTTAGCGATAGCGATGTACCATTTCACTGAAAGCC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAgg < 1:918647/239‑1 (MQ=255) aGTCTACTAATTTGCTTCCCAGCGTGTCGAGGGTTGGCGAA‑ACG‑CCGGCCGGCGTTGAGCGATAGCGATGTCCCATTTCCCGAATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAggc < 1:294285/239‑1 (MQ=255) aCTAATTTGCTTTCCAGCGCGGCGAGGGCGTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGGCCCATTTCACTGATAACC‑GCCAGCTAAGGAACTTCCCAGAGGATGACTTCACGCCACGGTCCGCTTAAACGAATATCCGGC‑CTGCCTTTATCCTCGGACACGGTGAGTTGGTCCGGGTTAAACCGGAACTTGACTCACCAGTTTAGGAAGTGGGCCTTGACAGAAggcagggc > 2:1111617/1‑239 (MQ=255) cTTTCCAGCGGGTCGGGGGCTTGCAAA‑ACG‑TGGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa < 1:605814/239‑1 (MQ=255) tttCCGGCGGGTCGAGGGCTGGCGCA‑ACG‑TCGCCCGGGGGTGAGCGATAGCGATGTACCATTTCACGGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCa < 1:80632/238‑1 (MQ=255) cAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGat < 1:362211/239‑1 (MQ=255) gtgtAGAGCGCTTGGGCA‑GCG‑CGGCCCTGGGGTGAGGGATAGAGATGTACCTTTCCCCGGAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATAtt < 1:1324783/238‑1 (MQ=255) tgtCGGGGTCTTGCGCA‑AGG‑TCGGCCTGCGGTGAGCGAGAGGGATGTGCCATTCCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtc < 2:650234/238‑1 (MQ=255) tgtCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATtca > 2:594722/1‑239 (MQ=255) ttGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCa > 2:291113/1‑239 (MQ=255) gcgcA‑ACG‑TCGGCCTGGGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAgg < 1:325606/239‑1 (MQ=255) gcgcA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAg > 1:634120/1‑238 (MQ=255) gcA‑ACG‑CCGGCCTGCGGTGGGCGATAGCGATGTACCATTCCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGgcg < 1:1049122/239‑1 (MQ=255) cG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGA‑TTGCAATTATCGTTGGACACGGTGACTTGTTCCGGGCTAAAGCTGAACTCGCGTAACCAGATAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCAATGATATTTATCATCCTGCATgctccgg > 2:1278089/1‑234 (MQ=255) g‑TCGGCCTGCGGTGAGCTATAGCAATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGt < 1:849248/239‑1 (MQ=255) cggccggcGGTGAGCGATACCGAGGTACCATTACACGAAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTAAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtg < 1:1036121/238‑1 (MQ=255) cggCGGAGGGCGATGGCGTTATCCCTTTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 2:1170241/237‑1 (MQ=255) cTGGGGTGAGCGATAGCGATGTACCATTTCACTAATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa < 1:1241610/239‑1 (MQ=255) cTGCGGTGAGCGATAGCGATGTCCCATTTCACTGAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGtgctgc < 1:761944/238‑1 (MQ=255) tGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCa > 1:1011007/1‑238 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa < 1:1234413/156‑1 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTaa > 2:1234413/1‑156 (MQ=255) cGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATc > 2:1190366/1‑238 (MQ=255) gAGCGATAGCGATGTACCATTGCACAGAAAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGACTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTg < 1:1051256/239‑1 (MQ=255) | TCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCATCAGATGGAAGCGCGACATATCAAGACCGGCGGTTAACGCCGAGAAGTCGACTAATTTGCTTTCCAGCGTGTCGAGGGCTTGCGCA‑ACG‑TCGGCCTGCGGTGAGCGATAGCGATGTACCATTTCACTGATAACC‑GCCAGCAAAGGAACTTCCCAGAGGATGACTTCACGCCACGGGCCGCTTAAACGAATATCCAGC‑TTGCCATTATCGTTGGACACGGTGACTTGTTCCGGGTTAAAGCGGAACTCGCGTAACCAGTTAAGATAGTCGGCCTTAAAGAAAGGCAGGGCAGAAAGCCACTGATATTCATCATCCTGCAGGCGCAGGTGCTGCATCGCCTG > NC_000913/989709‑990182 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |