Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,758 G→C 8.8% E614Q (GAA→CAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,7580GC 8.8% 152.0 / 3.1 66E614Q (GAA→CAA) rsxCSoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (36/22);  new base C (3/3);  total (41/25)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGC  >  NC_000913/1708523‑1708993
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            
cAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaaga                                                                                                                                                                                                                                             <  1:1206975/237‑1 (MQ=255)
cAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTAGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaaga                                                                                                                                                                                                                                             <  1:1350647/237‑1 (MQ=255)
  gagCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAg                                                                                                                                                                                                                                         >  1:490247/1‑239 (MQ=255)
   agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCGGCTATTGCCCGTGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAg                                                                                                                                                                                                                                         >  2:1252393/1‑238 (MQ=255)
   agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                                                                                                                                        >  1:320723/1‑239 (MQ=255)
   agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                                                                                                                                        >  1:1405592/1‑239 (MQ=255)
        aaCAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                                                                                                                                                                   <  1:1400812/239‑1 (MQ=255)
                    tGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgcc                                                                                                                                                                                                                       >  1:829277/1‑239 (MQ=255)
                      cagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCCCGAAAACTGGACCAGCAAAAGGCTGATACCGAACCAGAAAACCAGGTCGTTCCCCCCAAAgccg                                                                                                                                                                                                                      >  2:6193/1‑238 (MQ=18)
                      cagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGACCAGCAACCGGCTAATGCGGAACCAGAAGAACAGGTCGATCCCCGCAAAgccg                                                                                                                                                                                                                      >  1:386034/1‑238 (MQ=255)
                           aaCTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGGGCCAAAGCGCGCAAAATGGAACAGCAACAGGCTACTGCGGAAACAGAAGCACAGGTCGATCCGAACAAAACAGCCGTCg                                                                                                                                                                                                                >  2:73520/1‑239 (MQ=18)
                                      cagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGTAACCAGAAGAACAGGTCGATCCCCGCAAAGCCGCCGACGAAGCCCCTAtt                                                                                                                                                                                                     >  1:819935/1‑239 (MQ=255)
                                              gTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTCCCCGTg                                                                                                                                                                                              >  1:91151/1‑238 (MQ=255)
                                                 gCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGATCCAGAAGAACACGTCGACCCGCGCAACGCCGCCGTCCACGCGGCTATTGCCAGCGccca                                                                                                                                                                                          >  2:1014432/1‑237 (MQ=17)
                                                 gCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaa                                                                                                                                                                                          >  1:182660/1‑239 (MQ=255)
                                                      tCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATTCGAGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCAGCCACAAACGGCGGTCGCAGCGACTACTGCCCGTGCCAAAGCAc                                                                                                                                                                                     >  2:1033369/1‑239 (MQ=17)
                                                          cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGAAAACTGGACCAGCAACAGGCTAATGCGGAACCAGAAGAACCGGTCGATCCGCGCAAAGCCGCAGACGAAGCGGCTATTGCCCggccacaagcgcgaaa                                                                                                                                                                                 >  2:74991/1‑224 (MQ=14)
                                                             aaaaCTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGc                                                                                                                                                                              <  1:1323040/238‑1 (MQ=255)
                                                                    ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaaga                                                                                                                                                                                                                                             <  1:1210052/169‑1 (MQ=255)
                                                                    ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaaga                                                                                                                                                                                                                                             >  2:1210052/1‑169 (MQ=255)
                                                                    ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTCGCAGCGGCTCTTGCCCTCGCCAAAGCACCCCAGCTGGcaca                                                                                                                                                                       >  2:478294/1‑238 (MQ=14)
                                                                     cGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGACCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAACCGCCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCAGGCCAGCGTGAACAGc                                                                                                                                                                     >  2:701124/1‑239 (MQ=14)
                                                                        tGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGACCAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                   <  1:208952/238‑1 (MQ=255)
                                                                        tGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                   <  1:240610/238‑1 (MQ=255)
                                                                              agcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCt                                                                                                                                                             <  1:1078485/238‑1 (MQ=255)
                                                                                 tatgggcgctgacaaaaagcgcAAACGAGAACAGCAACAGGCTCATGCCGAGCCAGAACACCAGCTCGATCCGCGCAACGCCGGCGTGGAAGCCGCGAGTGCGCGAGCCAAAGCGAGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATg                                                                                                                                                         <  2:1254558/221‑1 (MQ=14)
                                                                                  aTCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                        <  1:28777/239‑1 (MQ=255)
                                                                                   tcgacgtcgccgaaagccgcAAGCGGGAAGAGCAACAGGTAAATAACGAGCCAGAACACCAGGACGACCGGCGAAAAGCGGCCGCCGAAGCCGCTATCGCCCGTGCAAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                        <  2:256993/221‑1 (MQ=14)
                                                                                       cagagcaaaaGGGGGCAAGCGGAAACAGCAACAGGAAAATGAGGACCCAGAACAACAGGCAGATGCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGc                                                                                                                                                   <  2:1202239/232‑1 (MQ=14)
                                                                                         tggcgaaaGCGCTCAAGCGGGAAATGAGATAGGTAAACGCGGAACCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCAAAAGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa                                                                                                                                                 <  2:182660/234‑1 (MQ=14)
                                                                                         gcgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCCAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCCCCGTTGACGCGGCTATGGACCGTGCGACGGCCCGCACACTGGAGCAGCAACAGGCTacggcggcgccc                                                                                                                                                 >  2:1139505/1‑228 (MQ=14)
                                                                                          tgcCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAACGGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTAGTAGCCGATATTCCCCGTGCCAAAGCACACAAACTGCAGCAGCAACACGCTAATCACGAGccca                                                                                                                                                >  2:1022766/2‑237 (MQ=11)
                                                                                          cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCGACCCAg                                                                                                                                                >  1:32654/1‑239 (MQ=255)
                                                                                          cgcCAAAGCGCGCAAGCTCGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAACCGCCGTCGACGCCGCTATTCCCCGTGCCCAAACGCGCAAGTTGGACCAGCAACAGGCTAATGCGGAACCCGAACAACAGGTCGATCCACGACAAGCCGCCGTTGAGGCAGCCATTGCCTGTGCCAAATCACTCCAAGCGGACCAGCAACAgcttcctcatgtttcag                                                                                                                                                >  2:691633/1‑223 (MQ=11)
                                                                                              aaaGCCGGCAAGAAGGAACAGCAACAGGAACATGAGAAAGCAGAACACCAGGCCGAACCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                                                                                                                                            <  2:763711/239‑1 (MQ=14)
                                                                                                    cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACACAAGATGGAACAGCAACCGGCTACTGCCGACGCAAACCAACAga                                                                                                                                       >  2:1370547/1‑237 (MQ=14)
                                                                                                                   cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAAGAACAGGTCGATCCGCCCAAAg                                                                                                                        >  1:266038/1‑238 (MQ=255)
                                                                                                                   cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACTGGACCAGCAGCAGGCTAATGCGCAGCCACAAAAACAGGTCGGTCCGCGCAAACCCGCCGTCGAGGCGGCTATTCCCCGTGCCAAGGCACGCAAGCCGAAAAAGCAACGGTCGAATACCGCGCCCGaaaacacggctaccccacccaaac                                                                                                                        >  2:310571/1‑216 (MQ=2)
                                                                                                                        ggCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTCGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGACCAGCAACAGGCTAATGCCGCGCCAGAACAACAGGTCGATCCGCGCAAAGccgccg                                                                                                                  >  1:576212/1‑239 (MQ=255)
                                                                                                                             tagCGGGACCAGAACACCAGGACGCTCGGCGGAAAGCCGCCGGCGAAGCAGCTATCGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGaa                                                                                                             <  2:1380024/237‑1 (MQ=14)
                                                                                                                              tGCGGACCCGGACAAACGGGTGGATCCGCAGAAAGCCGCCAGTGAAGCCGCAAGTGCCCGGGCCAAAGGGCGGAAGCTGGAACAGCAACAGGCGAAGGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                            <  2:1405592/239‑1 (MQ=14)
                                                                                                                                         aacaacAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGc                                                                                                  >  1:1214964/1‑238 (MQ=255)
                                                                                                                                         aacaacAGGTCGATCCGCGCAAAGACGCCGTCGAAGCCGCTATTGCTCGTGCCAAAGCGCGCAAGCAGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGACGACGCCGCTATTGCCCGGGCCAAAGCGGCAAAGAGGGAAGCGCAACAGGCTGATACAGCGCCGCAACAGCAGACCGAGCCGCGGATAGccgccggcacatacacactcgc                                                                                                  >  2:995044/1‑222 (MQ=9)
                                                                                                                                                 gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGACGATCCGCGCAAAGCAGCCGTCGCTGCCGCTATTGCCCgcaccca                                                                                         >  1:1006619/1‑233 (MQ=14)
                                                                                                                                                   ggaccgaccaaatactgccgccGAAGCCGCTATGGCCCGAGCAAGAGCGGGAAAGCTGGAACAGCAACAGGATAATGCGGAACAAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGGCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCaaa                                                                                        <  2:210456/222‑1 (MQ=14)
                                                                                                                                                   cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCaaa                                                                                        >  1:845688/1‑238 (MQ=255)
                                                                                                                                                                       ctgAAGCCGCGATTGCGCGTGCCCAAGCGCTCCGGCTGTACAGACGACGGGCTATTGCGGAACCAGCAGACGTGGCCGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCaa                                                                   <  2:819935/237‑1 (MQ=14)
                                                                                                                                                                         gAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGACGCAGCTATTGCCCGCGCTCAAGCCAGCAACCAGGACCAGCAAAAGGCTAATGCGGACCCCGAAGACCAGGGCGATCCGCGCAAAGCCGCCGTAGCCGCGAATATTGCCCGAGCCAAAAGACGCAAGCggcgccagcacac                                                                 >  2:823621/1‑228 (MQ=2)
                                                                                                                                                                              cGCTATTGCCCGCGCAAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCt                                                             <  1:770740/238‑1 (MQ=255)
                                                                                                                                                                                           gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga                                               >  1:1117526/1‑239 (MQ=255)
                                                                                                                                                                                           gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGACCGAGCCAAAGCGCGACAGCCGGAACAGCACACGGCCACATCGGAGCCaga                                               >  2:624478/1‑239 (MQ=14)
                                                                                                                                                                                              aaaGGGAGCAAGCAGGAACAGCAACAGGCAAATAAGGAGCCAGAACAAGAGGCGGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATGGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                            <  2:1292618/239‑1 (MQ=11)
                                                                                                                                                                                              aaaGCGGGAAAGCTGAAAAAGCAACAGGAAAATACGGACGCAGAACAACAGGTCGATCCGGGAAAAACCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                            <  2:1271610/239‑1 (MQ=14)
                                                                                                                                                                                               aaGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                            >  2:692520/1‑238 (MQ=255)
                                                                                                                                                                                                aGCGCGCAAGCTGGAACAGCAACATGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAc                                          >  1:304023/1‑239 (MQ=255)
                                                                                                                                                                                                    cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCGAGCCCGAACAACAGGTCGATCCGCGCAAAACCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCAGCAAACCGGACCAGCACCCGGCTAACACGGAgaccgcacaaccagt                                      >  2:1230272/1‑225 (MQ=14)
                                                                                                                                                                                                    cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGACCAGCAACCGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAAAGACCGCAAACGGGAACAGCAACCGGCTATTGAGACACCAGACGAACAggc                                      >  2:482862/1‑238 (MQ=14)
                                                                                                                                                                                                     gcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                                                                                                                                     <  2:188937/127‑1 (MQ=38)
                                                                                                                                                                                                      cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                                                                                                                                  <  1:1349681/145‑1 (MQ=255)
                                                                                                                                                                                                      cAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                                                                                                                                  >  2:1349681/1‑145 (MQ=255)
                                                                                                                                                                                                                    cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGc                       >  1:1020000/1‑239 (MQ=255)
                                                                                                                                                                                                                    cAACAGGCTAATGCCCAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGTCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCTCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                            <  1:122259/218‑1 (MQ=11)
                                                                                                                                                                                                                     aaCAGGCTACTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCt                      <  1:1057591/239‑1 (MQ=255)
                                                                                                                                                                                                                         ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTccc                   >  1:1254558/1‑238 (MQ=255)
                                                                                                                                                                                                                             actGCGGAAGCAGAGGAACAGGCCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa              <  1:1317985/237‑1 (MQ=255)
                                                                                                                                                                                                                              aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAAc                                                                                                                                                                        <  1:386348/84‑1 (MQ=38)
                                                                                                                                                                                                                              aTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCAGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATGCTCGCAAAGCTGCCGACGAAGCGGTTCTTGCCAGAGCCAAAGCGCGCAGACGGGCACAGGCACCGGCTATTGAGGAGCCCGAAAAAAAGGTAGAtactgcctcttatccatctct              >  2:122259/1‑218 (MQ=2)
                                                                                                                                                                                                                               tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGaa             >  1:351467/1‑238 (MQ=255)
                                                                                                                                                                                                                               tGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg            <  1:758100/239‑1 (MQ=255)
                                                                                                                                                                                                                                    aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCTGCGCAAAGCCGCCGTCGAAGCCGCt       >  1:718555/1‑239 (MQ=255)
                                                                                                                                                                                                                                         gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGc  >  1:932648/1‑239 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            
CAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGC  >  NC_000913/1708523‑1708993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: